Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0005635

Physical Location & Seq

Genomic Coordinates Reverse (-)

83955631 .. 83959837

Gene Structure 4207 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G048300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 840 bp

ATGACCACATTCATAAGCTGCATATTCAGTTTTTCGGAAACTACCAAACTGATAAGATTTCTTTATTCTTGGGAGTGGGTTCAAATGTTGGAGCGTCCCCCAACATGGTCTCTTCTAGGACATAACCATAATCCGGATTTATCTCTTTCTGCAGCTGAAGCTGCCAAACTGGCTTCAAAGTATTGCAAAGAAGGATTTAAAACTTTAAAGCTTAAGGTAGGAAAGAATCTAAATTCAGACATAGAAGTCCTTCAAGCTATAAGAGCAGTCCACCCTGATTGCTCTTTTATCTTGGACGCTAACGAAGGATATAGCTCCAATGAAGCTATCCAAGTTCTTGAGAAATTATATGAGGTGGGAGTGAGTCCAGTTCTCTTTGAACAACCAGTTCATAGAGATGATTGGGAAGGCCTTCGATATGTTACTCGTATTGCTAGAGAAAGATATGGAGTATCAGTTGCAGCTGCTGAGAGCTGTCGAAGTATAGTGGATGTTAAGAAAATAGCAGAAGAAAATCTTGCTAATGTCATTGACATTAAACTTACTAAAGTTGGGGTTGTTGGGGCCCTTGAAATCATTGAGGTTGCAAGAGCATCGGGATTGGATTTGATGATTGGTGGTATGGTTGAGACTAGACTCGCCATGGGCTTTTCTGGCCACCTTGCTGCTGGCCTTGGGTGTTTTAAGTTTATTGATCTAGATACGCCCCTATTGCTGTCAGAAGATCCAGTTCTGGAGGGTTATGAAGTTTCGGGTGCCGTTTATAAGTTCAACAACGCTAGAGGCCATGGTGGATTTCTCCACTGGGACAACCTTGCTTGGCAAGTCATAAAAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016853 GO:0016854 GO:0016855 GO:0034641 GO:0043167 GO:0043169 GO:0043603 GO:0044237 GO:0046872 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MR_MLE_C MR_MLE_N

Protein Analysis

279

Amino Acids

30.98

Weight (kDa)

5.7

Isoelectric Point (pI)

19.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MR_MLE_C	PF13378	54 - 264	1.1e-33	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005635)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18270
fragaria_vesca	FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670
malus_domestica	MD06G1220000.v1.1 MD14G1229600.v1.1
prunus_persica	Prupe.5G224400_v2.0.a1 Prupe.5G224400_v2.0.a1
pyrus_communis	pycom14g19100 pycom14g19110
rosa_chinensis	RchiOBHm_Chr7g0179451
rosa_laevigata	RLG00000005355
rosa_multiflora	Rmu_co8470985.1_g000001
rosa_roxburghii	Rroxscaffold_3G00274010
rosa_rugosa	Rorug06G0424300 Rorug06G0424400 Rorug06G0424500 Rorug06G0424600
rosa_samantha	Rh2AG318700 Rh6AG144500 Rh7BG023500
rosa_wichuraiana	Rw4G009460 Rw4G033460 Rw5G048300 Rw7G001940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	765
AasI	GACNNNNNNGTC	1 cut(s)	245
AccB1I	GGYRCC	1 cut(s)	755
AccIII	TCCGGA	1 cut(s)	133
AclWI	GGATC	1 cut(s)	719
AcoI	YGGCCR	1 cut(s)	655
AcsI	RAATTY	1 cut(s)	232
AcuI	CTGAAG	1 cut(s)	177
AfiI	CCNNNNNNNGG	2 cut(s)	105, 133
AflII	CTTAAG	1 cut(s)	212
AgsI	TTSAA	6 cut(s)	83, 177, 254, 380, 572, 772
AluBI	AGCT	9 cut(s)	18, 155, 161, 211, 257, 315, 326, 464, 474
AluI	AGCT	9 cut(s)	18, 155, 161, 211, 257, 315, 326, 464, 474
Alw26I	GTCTC	2 cut(s)	114, 623
AlwI	GGATC	1 cut(s)	719
AlwNI	CAGNNNCTG	1 cut(s)	467
Aor13HI	TCCGGA	1 cut(s)	133
AoxI	GGCC	5 cut(s)	409, 564, 655, 670, 784
ApaI	GGGCCC	1 cut(s)	568
ApeKI	GCWGC	6 cut(s)	18, 152, 161, 461, 464, 665
ApoI	RAATTY	1 cut(s)	232
Asp700I	GAANNNNTTC	2 cut(s)	249, 411
AspS9I	GGNCC	2 cut(s)	564, 565
BaeGI	GKGCMC	1 cut(s)	568
BalI	TGGCCA	1 cut(s)	657
BanI	GGYRCC	1 cut(s)	755
BanII	GRGCYC	1 cut(s)	568
BbvI	GCAGC	6 cut(s)	5, 148, 164, 451, 473, 652
BceAI	ACGGC	1 cut(s)	743
BcgI	CGANNNNNNTGC	2 cut(s)	576, 610
BcoDI	GTCTC	2 cut(s)	114, 623
BfaI	CTAG	5 cut(s)	116, 435, 633, 698, 780
BfmI	CTRYAG	1 cut(s)	150
BfrI	CTTAAG	1 cut(s)	212
BglI	GCCNNNNNGGC	1 cut(s)	170
BisI	GCNGC	6 cut(s)	19, 153, 162, 462, 465, 666
BlsI	GCNGC	6 cut(s)	20, 154, 163, 463, 466, 667
BmgT120I	GGNCC	2 cut(s)	564, 565
BmiI	GGNNCC	3 cut(s)	565, 566, 757
BmrI	ACTGGG	1 cut(s)	814
BmsI	GCATC	1 cut(s)	602
BmuI	ACTGGG	1 cut(s)	814
BpmI	CTGGAG	1 cut(s)	755
BpuEI	CTTGAG	1 cut(s)	359
BsaI	GGTCTC	1 cut(s)	114
BsaJI	CCNNGG	3 cut(s)	642, 673, 787
BsaWI	WCCGGW	1 cut(s)	133
BsaXI	ACNNNNNCTCC	2 cut(s)	441, 471
Bsc4I	CCNNNNNNNGG	2 cut(s)	105, 133
Bse1I	ACTGG	5 cut(s)	174, 368, 386, 728, 809
BseAI	TCCGGA	1 cut(s)	133
BseDI	CCNNGG	3 cut(s)	642, 673, 787
BseGI	GGATG	1 cut(s)	496
BseLI	CCNNNNNNNGG	2 cut(s)	105, 133
BseMII	CTCAG	1 cut(s)	459
BseNI	ACTGG	5 cut(s)	174, 368, 386, 728, 809
BseSI	GKGCMC	1 cut(s)	568
BseXI	GCAGC	6 cut(s)	5, 148, 164, 451, 473, 652
BshFI	GGCC	5 cut(s)	411, 566, 657, 672, 786
BshNI	GGYRCC	1 cut(s)	755
BsiSI	CCGG	1 cut(s)	134
BslFI	GGGAC	2 cut(s)	81, 821
BslI	CCNNNNNNNGG	2 cut(s)	105, 133
BsmAI	GTCTC	2 cut(s)	114, 623
BsmFI	GGGAC	2 cut(s)	81, 821
BsnI	GGCC	5 cut(s)	411, 566, 657, 672, 786
Bso31I	GGTCTC	1 cut(s)	114
Bsp120I	GGGCCC	1 cut(s)	564
Bsp1286I	GDGCHC	1 cut(s)	568
Bsp13I	TCCGGA	1 cut(s)	133
Bsp143I	GATC	2 cut(s)	694, 724
Bsp19I	CCATGG	2 cut(s)	642, 787
BspANI	GGCC	5 cut(s)	411, 566, 657, 672, 786
BspCNI	CTCAG	1 cut(s)	460
BspEI	TCCGGA	1 cut(s)	133
BspLI	GGNNCC	3 cut(s)	565, 566, 757
BspMAI	CTGCAG	1 cut(s)	154
BspPI	GGATC	1 cut(s)	719
BspT107I	GGYRCC	1 cut(s)	755
BspTI	CTTAAG	1 cut(s)	212
BspTNI	GGTCTC	1 cut(s)	114
BsrI	ACTGG	5 cut(s)	174, 368, 386, 728, 809
BssECI	CCNNGG	3 cut(s)	642, 673, 787
BssMI	GATC	2 cut(s)	694, 724
BssT1I	CCWWGG	3 cut(s)	642, 673, 787
Bst6I	CTCTTC	1 cut(s)	117
BstAFI	CTTAAG	1 cut(s)	212
BstC8I	GCNNGC	1 cut(s)	670
BstDEI	CTNAG	1 cut(s)	468
BstDSI	CCRYGG	2 cut(s)	642, 787
BstF5I	GGATG	1 cut(s)	496
BstKTI	GATC	2 cut(s)	697, 727
BstMAI	GTCTC	2 cut(s)	114, 623
BstMBI	GATC	2 cut(s)	694, 724
BstMWI	GCNNNNNNNGC	6 cut(s)	158, 161, 170, 263, 654, 712
BstSFI	CTRYAG	1 cut(s)	150
BstSLI	GKGCMC	1 cut(s)	568
BstV1I	GCAGC	6 cut(s)	5, 148, 164, 451, 473, 652
BstX2I	RGATCY	1 cut(s)	724
BstYI	RGATCY	1 cut(s)	724
BsuRI	GGCC	5 cut(s)	411, 566, 657, 672, 786
BtgI	CCRYGG	2 cut(s)	642, 787
BtsCI	GGATG	1 cut(s)	496
BtsIMutI	CAGTG	1 cut(s)	802
Cac8I	GCNNGC	1 cut(s)	670
CaiI	CAGNNNCTG	1 cut(s)	467
Cfr13I	GGNCC	2 cut(s)	564, 565
CseI	GACGC	2 cut(s)	83, 305
CspCI	CAANNNNNGTGG	2 cut(s)	260, 295
CviAII	CATG	3 cut(s)	105, 643, 788
DdeI	CTNAG	1 cut(s)	468
DpnI	GATC	2 cut(s)	696, 726
DpnII	GATC	2 cut(s)	694, 724
DraI	TTTAAA	2 cut(s)	199, 207
DrdI	GACNNNNNNGTC	1 cut(s)	245
DseDI	GACNNNNNNGTC	1 cut(s)	245
EaeI	YGGCCR	1 cut(s)	655
Eam1104I	CTCTTC	1 cut(s)	117
EarI	CTCTTC	1 cut(s)	117
Eco130I	CCWWGG	3 cut(s)	642, 673, 787
Eco147I	AGGCCT	1 cut(s)	411
Eco24I	GRGCYC	1 cut(s)	568
Eco31I	GGTCTC	1 cut(s)	114
Eco57I	CTGAAG	1 cut(s)	177
EcoO109I	RGGNCCY	2 cut(s)	564, 565
EcoT14I	CCWWGG	3 cut(s)	642, 673, 787
EcoT38I	GRGCYC	1 cut(s)	568
ErhI	CCWWGG	3 cut(s)	642, 673, 787
FaeI	CATG	3 cut(s)	108, 646, 791
FalI	AAGNNNNNCTT	2 cut(s)	501, 533
FaqI	GGGAC	2 cut(s)	81, 821
FatI	CATG	3 cut(s)	104, 642, 787
Fnu4HI	GCNGC	6 cut(s)	19, 153, 162, 462, 465, 666
FokI	GGATG	1 cut(s)	503
FriOI	GRGCYC	1 cut(s)	568
Fsp4HI	GCNGC	6 cut(s)	19, 153, 162, 462, 465, 666
FspBI	CTAG	5 cut(s)	116, 435, 633, 698, 780
GluI	GCNGC	6 cut(s)	19, 153, 162, 462, 465, 666
GsuI	CTGGAG	1 cut(s)	755
HaeIII	GGCC	5 cut(s)	411, 566, 657, 672, 786
HapII	CCGG	1 cut(s)	134
HgaI	GACGC	2 cut(s)	83, 305
Hin1II	CATG	3 cut(s)	108, 646, 791
HindIII	AAGCTT	1 cut(s)	209
HinfI	GANTC	3 cut(s)	226, 364, 636
HpaII	CCGG	1 cut(s)	134
Hpy166II	GTNNAC	1 cut(s)	271
Hpy188I	TCNGA	3 cut(s)	37, 238, 721
Hpy188III	TCNNGA	5 cut(s)	134, 338, 597, 698, 734
Hpy8I	GTNNAC	1 cut(s)	271
HpyAV	CCTTC	5 cut(s)	185, 260, 299, 401, 422
HpyCH4V	TGCA	5 cut(s)	21, 152, 186, 461, 587
HpyF10VI	GCNNNNNNNGC	6 cut(s)	158, 161, 170, 263, 654, 712
HpyF3I	CTNAG	1 cut(s)	468
Hsp92II	CATG	3 cut(s)	108, 646, 791
Kpn2I	TCCGGA	1 cut(s)	133
Kzo9I	GATC	2 cut(s)	694, 724
LmnI	GCTCC	2 cut(s)	91, 320
Lsp1109I	GCAGC	6 cut(s)	5, 148, 164, 451, 473, 652
LweI	GCATC	1 cut(s)	602
MaeI	CTAG	5 cut(s)	116, 435, 633, 698, 780
MaeIII	GTNAC	1 cut(s)	421
MalI	GATC	2 cut(s)	696, 726
MboI	GATC	2 cut(s)	694, 724
MboII	GAAGA	3 cut(s)	104, 521, 734
MflI	RGATCY	1 cut(s)	724
MhlI	GDGCHC	1 cut(s)	568
MlsI	TGGCCA	1 cut(s)	657
MluCI	AATT	2 cut(s)	232, 344
MluNI	TGGCCA	1 cut(s)	657
MlyI	GAGTC	2 cut(s)	373, 630
MmeI	TCCRAC	1 cut(s)	69
MnlI	CCTC	4 cut(s)	346, 574, 730, 776
Mox20I	TGGCCA	1 cut(s)	657
MroI	TCCGGA	1 cut(s)	133
MroXI	GAANNNNTTC	2 cut(s)	249, 411
MscI	TGGCCA	1 cut(s)	657
MseI	TTAA	6 cut(s)	198, 206, 213, 495, 537, 684
MslI	CAYNNNNRTG	1 cut(s)	396
Msp20I	TGGCCA	1 cut(s)	657
MspA1I	CMGCKG	2 cut(s)	155, 464
MspCI	CTTAAG	1 cut(s)	212
MspI	CCGG	1 cut(s)	134
MwoI	GCNNNNNNNGC	6 cut(s)	158, 161, 170, 263, 654, 712
NcoI	CCATGG	2 cut(s)	642, 787
NdeII	GATC	2 cut(s)	694, 724
NlaIII	CATG	3 cut(s)	108, 646, 791
NlaIV	GGNNCC	3 cut(s)	565, 566, 757
PceI	AGGCCT	1 cut(s)	411
PdmI	GAANNNNTTC	2 cut(s)	249, 411
PfeI	GAWTC	1 cut(s)	226
PkrI	GCNGC	6 cut(s)	20, 154, 163, 463, 466, 667
PleI	GAGTC	2 cut(s)	372, 630
PpsI	GAGTC	2 cut(s)	372, 630
PsiI	TTATAA	1 cut(s)	765
PspN4I	GGNNCC	3 cut(s)	565, 566, 757
PspOMI	GGGCCC	1 cut(s)	564
PspPI	GGNCC	2 cut(s)	564, 565
PstI	CTGCAG	1 cut(s)	154
PstNI	CAGNNNCTG	1 cut(s)	467
PsuI	RGATCY	1 cut(s)	724
PvuII	CAGCTG	2 cut(s)	155, 464
RseI	CAYNNNNRTG	1 cut(s)	396
SaqAI	TTAA	6 cut(s)	198, 206, 213, 495, 537, 684
SatI	GCNGC	6 cut(s)	19, 153, 162, 462, 465, 666
Sau3AI	GATC	2 cut(s)	694, 724
Sau96I	GGNCC	2 cut(s)	564, 565
SchI	GAGTC	2 cut(s)	373, 630
SduI	GDGCHC	1 cut(s)	568
SfaNI	GCATC	1 cut(s)	602
SfcI	CTRYAG	1 cut(s)	150
SmiMI	CAYNNNNRTG	1 cut(s)	396
SmlI	CTYRAG	2 cut(s)	212, 338
SmoI	CTYRAG	2 cut(s)	212, 338
Sse9I	AATT	2 cut(s)	232, 344
SseBI	AGGCCT	1 cut(s)	411
SspMI	CTAG	5 cut(s)	116, 435, 633, 698, 780
StuI	AGGCCT	1 cut(s)	411
StyI	CCWWGG	3 cut(s)	642, 673, 787
TaqI	TCGA	2 cut(s)	415, 478
TasI	AATT	2 cut(s)	232, 344
TfiI	GAWTC	1 cut(s)	226
Tru1I	TTAA	6 cut(s)	198, 206, 213, 495, 537, 684
Tru9I	TTAA	6 cut(s)	198, 206, 213, 495, 537, 684
TscAI	CASTG	1 cut(s)	809
TseI	GCWGC	6 cut(s)	18, 152, 161, 461, 464, 665
TspDTI	ATGAA	3 cut(s)	336, 380, 759
TspRI	CASTG	1 cut(s)	809
Vha464I	CTTAAG	1 cut(s)	212
XapI	RAATTY	1 cut(s)	232
XbaI	TCTAGA	1 cut(s)	697
XcmI	CCANNNNNNNNNTGG	1 cut(s)	665
XmnI	GAANNNNTTC	2 cut(s)	249, 411
XspI	CTAG	5 cut(s)	116, 435, 633, 698, 780

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.