Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0005635

Physical Location & Seq

Genomic Coordinates Forward (+)

1575173 .. 1579152

Gene Structure 3980 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G001940.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1260 bp

ATGACCTCAATTGGGTTCTCTCTCAACTCCCTGATCCCTTGTTCTACTGTCAGTTCAACTCAGAGCTCAAAAAGACTGAGACCCAGTTCAAGAAGCAGTCCGAGAATGGCTCTGGCCACCCCCACCACCACATTTGGGTTCAGTAATTTACTCGAGACTTTCACAGTCAACGTTCAGAGAGCAGAGAACAGGCCTCTGAATGTGCCTCTGATTGCTCCTTTTACGATTGCGACTTCGAGGCTTGAACGTGTTGAGAATGTTGCTATCAGAGTTGAGTTGAGAAATGGGTGTGTGGGGTGGGGTGAAGCTCCAATCTTGCCTCATGTGACTGCAGAGGATCAGCACACCGCTATGGTCAAGGCCAAGGAGGCTTGTGAGTTCTTGCTCAGGAATAATGGGATGACTTTGAGTTCGGTTTTGGGAGAGATTGGTGTGCTTCTCCCTGGTTATGAGTTTGCTTCTGTTAGGGCAGGAGTCGAGATGGCTCTAATTGATGCAGTTGCTACAAGTATTGGTGTCCCTTTATGGAGACTGTTTGGTGGAGCTTCAAATACCATAACCACTGATATAACGATTCCGATAGTTTCTGCAGCTGAAGCTGCCAGACTGGCTTCAAAGTACTGCAAAGAAGGATTTAAAACTTTAAAGCTTAAGGTGGGAAAGAATCTAAATTCAGACATAGAAGTCCTTCAAGCTATACGGGCAGTCCACCCTGATTGCTCTTTTATCTTGGATGCTAACGAAGGATATAACTCCAATGAAGCTATCCAAGTTCTTGAGAAATTATATGAGGTGGGAGTGAGTCCAGTTCTCTTTGAACAACCAGTTCATAGAGATGATTGGGAAGGCCTTGGATATGTTACTCGTATTGCTAGAGAAAGATATGGAGTATCTGTTGCAGCTGATGAGAGCTGTCGAAGTATAGTCGATGTTAAGAAAATAGCAGAAGAAAATCTTGCTAATGTCATTAACATTAAACTTACTAAAGTTGGGGTTATTGGGGCCCTTGAAATCATTGAGGTTGCAAGAGCATCGGGATTGGATTTGATGATTGGTGGTATGGTTGAGACTAGACTCGCCATGGGCTTTTCTGGCCACCTTGCTGCTGGCCTTGGGTGTTTTAAGTTTATTGATCTAGATACGCCCCTATTGCTGTCAGAAGATCCAGTTCTGGAGGGTTATGAAGTTTCGGGTGCCGTTTATAAGTTCAACAACGCTAGAGGCCATGGTGGATTTCTCCACTGGGACAACCTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016853 GO:0016854 GO:0016855 GO:0034641 GO:0043167 GO:0043169 GO:0043603 GO:0044237 GO:0046872 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MR_MLE_C MR_MLE_N

Protein Analysis

419

Amino Acids

45.25

Weight (kDa)

5.64

Isoelectric Point (pI)

29.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MR_MLE_N	PF02746	69 - 180	4.3e-07	Mandelate racemase / muconate lactonizing enzyme, N-terminal domain
MR_MLE_C	PF13378	200 - 410	9.6e-34	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005635)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18270
fragaria_vesca	FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670
malus_domestica	MD06G1220000.v1.1 MD14G1229600.v1.1
prunus_persica	Prupe.5G224400_v2.0.a1 Prupe.5G224400_v2.0.a1
pyrus_communis	pycom14g19100 pycom14g19110
rosa_chinensis	RchiOBHm_Chr7g0179451
rosa_laevigata	RLG00000005355
rosa_multiflora	Rmu_co8470985.1_g000001
rosa_roxburghii	Rroxscaffold_3G00274010
rosa_rugosa	Rorug06G0424300 Rorug06G0424400 Rorug06G0424500 Rorug06G0424600
rosa_samantha	Rh2AG318700 Rh6AG144500 Rh7BG023500
rosa_wichuraiana	Rw4G009460 Rw4G033460 Rw5G048300 Rw7G001940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1203
AasI	GACNNNNNNGTC	1 cut(s)	683
AccB1I	GGYRCC	1 cut(s)	1193
AciI	CCGC	1 cut(s)	348
AclI	AACGTT	1 cut(s)	171
AclWI	GGATC	3 cut(s)	28, 345, 1157
AcoI	YGGCCR	2 cut(s)	114, 1093
AcsI	RAATTY	1 cut(s)	670
AcuI	CTGAAG	1 cut(s)	615
AfaI	GTAC	1 cut(s)	620
AfiI	CCNNNNNNNGG	2 cut(s)	12, 135
AflII	CTTAAG	1 cut(s)	650
AflIII	ACRYGT	1 cut(s)	247
AgsI	TTSAA	9 cut(s)	57, 90, 245, 549, 615, 692, 818, 1010, 1210
AjnI	CCWGG	1 cut(s)	442
Alw21I	GWGCWC	1 cut(s)	68
Alw26I	GTCTC	4 cut(s)	73, 149, 523, 1061
AlwI	GGATC	3 cut(s)	28, 345, 1157
Ama87I	CYCGRG	1 cut(s)	152
AoxI	GGCC	8 cut(s)	114, 191, 360, 847, 1002, 1093, 1108, 1222
ApaI	GGGCCC	1 cut(s)	1006
ApeKI	GCWGC	4 cut(s)	590, 599, 899, 1103
ApoI	RAATTY	1 cut(s)	670
ArsI	GACNNNNNNTTYG	2 cut(s)	394, 426
Asp700I	GAANNNNTTC	1 cut(s)	687
AspS9I	GGNCC	2 cut(s)	1002, 1003
AsuHPI	GGTGA	1 cut(s)	314
AvaI	CYCGRG	1 cut(s)	152
BaeGI	GKGCMC	1 cut(s)	1006
BalI	TGGCCA	2 cut(s)	116, 1095
BanI	GGYRCC	1 cut(s)	1193
BanII	GRGCYC	2 cut(s)	68, 1006
Bbv12I	GWGCWC	1 cut(s)	68
BbvI	GCAGC	4 cut(s)	586, 602, 911, 1090
BccI	CCATC	1 cut(s)	475
BceAI	ACGGC	1 cut(s)	1181
BcgI	CGANNNNNNTGC	2 cut(s)	1014, 1048
BciT130I	CCWGG	1 cut(s)	444
BcoDI	GTCTC	4 cut(s)	73, 149, 523, 1061
BfaI	CTAG	4 cut(s)	873, 1071, 1136, 1218
BfmI	CTRYAG	2 cut(s)	330, 588
BfrI	CTTAAG	1 cut(s)	650
BglI	GCCNNNNNGGC	2 cut(s)	368, 608
BisI	GCNGC	4 cut(s)	591, 600, 900, 1104
BlsI	GCNGC	4 cut(s)	592, 601, 901, 1105
BmcAI	AGTACT	1 cut(s)	620
Bme1390I	CCNGG	1 cut(s)	444
BmeT110I	CYCGRG	1 cut(s)	152
BmgT120I	GGNCC	2 cut(s)	1002, 1003
BmiI	GGNNCC	3 cut(s)	1003, 1004, 1195
BmrFI	CCNGG	1 cut(s)	444
BmrI	ACTGGG	2 cut(s)	78, 1252
BmsI	GCATC	3 cut(s)	484, 724, 1040
BmuI	ACTGGG	2 cut(s)	78, 1252
BplI	GAGNNNNNCTC	2 cut(s)	94, 126
BpmI	CTGGAG	1 cut(s)	1193
Bpu10I	CCTNAGC	1 cut(s)	386
BpuEI	CTTGAG	1 cut(s)	797
BsaI	GGTCTC	1 cut(s)	73
BsaJI	CCNNGG	6 cut(s)	363, 442, 850, 1080, 1111, 1225
BsaXI	ACNNNNNCTCC	2 cut(s)	879, 909
Bsc4I	CCNNNNNNNGG	2 cut(s)	12, 135
Bse1I	ACTGG	6 cut(s)	84, 612, 806, 824, 1166, 1247
BseBI	CCWGG	1 cut(s)	444
BseDI	CCNNGG	6 cut(s)	363, 442, 850, 1080, 1111, 1225
BseGI	GGATG	2 cut(s)	405, 739
BseLI	CCNNNNNNNGG	2 cut(s)	12, 135
BseMII	CTCAG	3 cut(s)	68, 74, 400
BseNI	ACTGG	6 cut(s)	84, 612, 806, 824, 1166, 1247
BseSI	GKGCMC	1 cut(s)	1006
BseXI	GCAGC	4 cut(s)	586, 602, 911, 1090
BshFI	GGCC	8 cut(s)	116, 193, 362, 849, 1004, 1095, 1110, 1224
BshNI	GGYRCC	1 cut(s)	1193
BsiHKAI	GWGCWC	1 cut(s)	68
BsiHKCI	CYCGRG	1 cut(s)	152
BslFI	GGGAC	1 cut(s)	503
BslI	CCNNNNNNNGG	2 cut(s)	12, 135
BsmAI	GTCTC	4 cut(s)	73, 149, 523, 1061
BsmFI	GGGAC	1 cut(s)	503
BsnI	GGCC	8 cut(s)	116, 193, 362, 849, 1004, 1095, 1110, 1224
Bso31I	GGTCTC	1 cut(s)	73
BsoBI	CYCGRG	1 cut(s)	152
Bsp120I	GGGCCC	1 cut(s)	1002
Bsp1286I	GDGCHC	2 cut(s)	68, 1006
Bsp143I	GATC	4 cut(s)	33, 337, 1132, 1162
Bsp19I	CCATGG	2 cut(s)	1080, 1225
BspACI	CCGC	1 cut(s)	348
BspANI	GGCC	8 cut(s)	116, 193, 362, 849, 1004, 1095, 1110, 1224
BspCNI	CTCAG	3 cut(s)	69, 73, 399
BspLI	GGNNCC	3 cut(s)	1003, 1004, 1195
BspMAI	CTGCAG	2 cut(s)	334, 592
BspPI	GGATC	3 cut(s)	28, 345, 1157
BspT107I	GGYRCC	1 cut(s)	1193
BspTI	CTTAAG	1 cut(s)	650
BspTNI	GGTCTC	1 cut(s)	73
BsrI	ACTGG	6 cut(s)	84, 612, 806, 824, 1166, 1247
BssECI	CCNNGG	6 cut(s)	363, 442, 850, 1080, 1111, 1225
BssMI	GATC	4 cut(s)	33, 337, 1132, 1162
BssT1I	CCWWGG	5 cut(s)	363, 850, 1080, 1111, 1225
Bst2UI	CCWGG	1 cut(s)	444
Bst4CI	ACNGT	3 cut(s)	49, 166, 534
BstAFI	CTTAAG	1 cut(s)	650
BstC8I	GCNNGC	1 cut(s)	1108
BstDEI	CTNAG	3 cut(s)	60, 77, 386
BstDSI	CCRYGG	2 cut(s)	1080, 1225
BstF5I	GGATG	2 cut(s)	405, 739
BstKTI	GATC	4 cut(s)	36, 340, 1135, 1165
BstMAI	GTCTC	4 cut(s)	73, 149, 523, 1061
BstMBI	GATC	4 cut(s)	33, 337, 1132, 1162
BstMWI	GCNNNNNNNGC	7 cut(s)	368, 596, 599, 608, 701, 1092, 1150
BstNI	CCWGG	1 cut(s)	444
BstSCI	CCNGG	1 cut(s)	442
BstSFI	CTRYAG	2 cut(s)	330, 588
BstSLI	GKGCMC	1 cut(s)	1006
BstV1I	GCAGC	4 cut(s)	586, 602, 911, 1090
BstX2I	RGATCY	1 cut(s)	1162
BstYI	RGATCY	1 cut(s)	1162
BsuRI	GGCC	8 cut(s)	116, 193, 362, 849, 1004, 1095, 1110, 1224
BtgI	CCRYGG	2 cut(s)	1080, 1225
BtsCI	GGATG	2 cut(s)	405, 739
BtsIMutI	CAGTG	2 cut(s)	561, 1240
Cac8I	GCNNGC	1 cut(s)	1108
Cfr13I	GGNCC	2 cut(s)	1002, 1003
Csp6I	GTAC	1 cut(s)	619
CspCI	CAANNNNNGTGG	4 cut(s)	115, 150, 698, 733
CviAII	CATG	3 cut(s)	323, 1081, 1226
CviQI	GTAC	1 cut(s)	619
DdeI	CTNAG	3 cut(s)	60, 77, 386
DpnI	GATC	4 cut(s)	35, 339, 1134, 1164
DpnII	GATC	4 cut(s)	33, 337, 1132, 1162
DraI	TTTAAA	2 cut(s)	637, 645
DrdI	GACNNNNNNGTC	1 cut(s)	683
DseDI	GACNNNNNNGTC	1 cut(s)	683
EaeI	YGGCCR	2 cut(s)	114, 1093
Ecl136II	GAGCTC	1 cut(s)	66
Eco130I	CCWWGG	5 cut(s)	363, 850, 1080, 1111, 1225
Eco147I	AGGCCT	2 cut(s)	193, 849
Eco24I	GRGCYC	2 cut(s)	68, 1006
Eco31I	GGTCTC	1 cut(s)	73
Eco53kI	GAGCTC	1 cut(s)	66
Eco57I	CTGAAG	1 cut(s)	615
Eco88I	CYCGRG	1 cut(s)	152
EcoICRI	GAGCTC	1 cut(s)	66
EcoO109I	RGGNCCY	2 cut(s)	1002, 1003
EcoRII	CCWGG	1 cut(s)	442
EcoT14I	CCWWGG	5 cut(s)	363, 850, 1080, 1111, 1225
EcoT38I	GRGCYC	2 cut(s)	68, 1006
ErhI	CCWWGG	5 cut(s)	363, 850, 1080, 1111, 1225
FaeI	CATG	3 cut(s)	326, 1084, 1229
FalI	AAGNNNNNCTT	2 cut(s)	939, 971
FaqI	GGGAC	1 cut(s)	503
FatI	CATG	3 cut(s)	322, 1080, 1225
Fnu4HI	GCNGC	4 cut(s)	591, 600, 900, 1104
FokI	GGATG	2 cut(s)	412, 746
FriOI	GRGCYC	2 cut(s)	68, 1006
Fsp4HI	GCNGC	4 cut(s)	591, 600, 900, 1104
FspBI	CTAG	4 cut(s)	873, 1071, 1136, 1218
GluI	GCNGC	4 cut(s)	591, 600, 900, 1104
GsuI	CTGGAG	1 cut(s)	1193
HaeIII	GGCC	8 cut(s)	116, 193, 362, 849, 1004, 1095, 1110, 1224
Hin1II	CATG	3 cut(s)	326, 1084, 1229
HincII	GTYRAC	1 cut(s)	169
HindII	GTYRAC	1 cut(s)	169
HindIII	AAGCTT	1 cut(s)	647
HinfI	GANTC	5 cut(s)	474, 574, 664, 802, 1074
HphI	GGTGA	1 cut(s)	314
Hpy166II	GTNNAC	2 cut(s)	169, 709
Hpy188I	TCNGA	9 cut(s)	63, 102, 177, 198, 210, 269, 579, 676, 1159
Hpy188III	TCNNGA	8 cut(s)	90, 154, 388, 478, 776, 1035, 1136, 1172
Hpy8I	GTNNAC	2 cut(s)	169, 709
HpyAV	CCTTC	4 cut(s)	623, 698, 737, 839
HpyCH4III	ACNGT	3 cut(s)	49, 166, 534
HpyCH4IV	ACGT	2 cut(s)	171, 247
HpyCH4V	TGCA	6 cut(s)	332, 497, 590, 624, 899, 1025
HpyF10VI	GCNNNNNNNGC	7 cut(s)	368, 596, 599, 608, 701, 1092, 1150
HpyF3I	CTNAG	3 cut(s)	60, 77, 386
HpySE526I	ACGT	2 cut(s)	171, 247
Hsp92II	CATG	3 cut(s)	326, 1084, 1229
Kzo9I	GATC	4 cut(s)	33, 337, 1132, 1162
LmnI	GCTCC	3 cut(s)	220, 313, 542
Lsp1109I	GCAGC	4 cut(s)	586, 602, 911, 1090
LweI	GCATC	3 cut(s)	484, 724, 1040
MaeI	CTAG	4 cut(s)	873, 1071, 1136, 1218
MaeII	ACGT	2 cut(s)	171, 247
MaeIII	GTNAC	2 cut(s)	325, 859
MalI	GATC	4 cut(s)	35, 339, 1134, 1164
MboI	GATC	4 cut(s)	33, 337, 1132, 1162
MboII	GAAGA	2 cut(s)	959, 1172
MfeI	CAATTG	1 cut(s)	9
MflI	RGATCY	1 cut(s)	1162
MhlI	GDGCHC	2 cut(s)	68, 1006
MlsI	TGGCCA	2 cut(s)	116, 1095
MluCI	AATT	5 cut(s)	9, 145, 489, 670, 782
MluNI	TGGCCA	2 cut(s)	116, 1095
MlyI	GAGTC	3 cut(s)	483, 811, 1068
Mox20I	TGGCCA	2 cut(s)	116, 1095
MroXI	GAANNNNTTC	1 cut(s)	687
MscI	TGGCCA	2 cut(s)	116, 1095
MseI	TTAA	7 cut(s)	636, 644, 651, 933, 969, 975, 1122
MslI	CAYNNNNRTG	2 cut(s)	350, 834
Msp20I	TGGCCA	2 cut(s)	116, 1095
MspA1I	CMGCKG	2 cut(s)	593, 902
MspCI	CTTAAG	1 cut(s)	650
MspR9I	CCNGG	1 cut(s)	444
MunI	CAATTG	1 cut(s)	9
MvaI	CCWGG	1 cut(s)	444
MwoI	GCNNNNNNNGC	7 cut(s)	368, 596, 599, 608, 701, 1092, 1150
NcoI	CCATGG	2 cut(s)	1080, 1225
NdeII	GATC	4 cut(s)	33, 337, 1132, 1162
NlaIII	CATG	3 cut(s)	326, 1084, 1229
NlaIV	GGNNCC	3 cut(s)	1003, 1004, 1195
NmuCI	GTSAC	1 cut(s)	325
PaeR7I	CTCGAG	1 cut(s)	152
PceI	AGGCCT	2 cut(s)	193, 849
PdmI	GAANNNNTTC	1 cut(s)	687
PfeI	GAWTC	2 cut(s)	574, 664
PkrI	GCNGC	4 cut(s)	592, 601, 901, 1105
PleI	GAGTC	3 cut(s)	482, 810, 1068
PpsI	GAGTC	3 cut(s)	482, 810, 1068
PsiI	TTATAA	1 cut(s)	1203
Psp124BI	GAGCTC	1 cut(s)	68
Psp1406I	AACGTT	1 cut(s)	171
Psp6I	CCWGG	1 cut(s)	442
PspGI	CCWGG	1 cut(s)	442
PspN4I	GGNNCC	3 cut(s)	1003, 1004, 1195
PspOMI	GGGCCC	1 cut(s)	1002
PspPI	GGNCC	2 cut(s)	1002, 1003
PstI	CTGCAG	2 cut(s)	334, 592
PsuI	RGATCY	1 cut(s)	1162
PvuII	CAGCTG	2 cut(s)	593, 902
RsaI	GTAC	1 cut(s)	620
RsaNI	GTAC	1 cut(s)	619
RseI	CAYNNNNRTG	2 cut(s)	350, 834
SacI	GAGCTC	1 cut(s)	68
SaqAI	TTAA	7 cut(s)	636, 644, 651, 933, 969, 975, 1122
SatI	GCNGC	4 cut(s)	591, 600, 900, 1104
Sau3AI	GATC	4 cut(s)	33, 337, 1132, 1162
Sau96I	GGNCC	2 cut(s)	1002, 1003
ScaI	AGTACT	1 cut(s)	620
SchI	GAGTC	3 cut(s)	483, 811, 1068
ScrFI	CCNGG	1 cut(s)	444
SduI	GDGCHC	2 cut(s)	68, 1006
SfaNI	GCATC	3 cut(s)	484, 724, 1040
SfcI	CTRYAG	2 cut(s)	330, 588
Sfr274I	CTCGAG	1 cut(s)	152
SlaI	CTCGAG	1 cut(s)	152
SmiMI	CAYNNNNRTG	2 cut(s)	350, 834
SmlI	CTYRAG	3 cut(s)	152, 650, 776
SmoI	CTYRAG	3 cut(s)	152, 650, 776
Sse9I	AATT	5 cut(s)	9, 145, 489, 670, 782
SseBI	AGGCCT	2 cut(s)	193, 849
SsiI	CCGC	1 cut(s)	348
SspMI	CTAG	4 cut(s)	873, 1071, 1136, 1218
SstI	GAGCTC	1 cut(s)	68
StuI	AGGCCT	2 cut(s)	193, 849
StyD4I	CCNGG	1 cut(s)	442
StyI	CCWWGG	5 cut(s)	363, 850, 1080, 1111, 1225
TaaI	ACNGT	3 cut(s)	49, 166, 534
TaiI	ACGT	2 cut(s)	174, 250
TaqI	TCGA	5 cut(s)	153, 236, 477, 916, 927
TasI	AATT	5 cut(s)	9, 145, 489, 670, 782
TatI	WGTACW	1 cut(s)	618
TfiI	GAWTC	2 cut(s)	574, 664
Tru1I	TTAA	7 cut(s)	636, 644, 651, 933, 969, 975, 1122
Tru9I	TTAA	7 cut(s)	636, 644, 651, 933, 969, 975, 1122
TscAI	CASTG	2 cut(s)	568, 1247
TseFI	GTSAC	1 cut(s)	325
TseI	GCWGC	4 cut(s)	590, 599, 899, 1103
Tsp45I	GTSAC	1 cut(s)	325
TspDTI	ATGAA	3 cut(s)	774, 818, 1197
TspRI	CASTG	2 cut(s)	568, 1247
Vha464I	CTTAAG	1 cut(s)	650
XapI	RAATTY	1 cut(s)	670
XbaI	TCTAGA	1 cut(s)	1135
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1103
XhoI	CTCGAG	1 cut(s)	152
XmnI	GAANNNNTTC	1 cut(s)	687
XspI	CTAG	4 cut(s)	873, 1071, 1136, 1218
ZrmI	AGTACT	1 cut(s)	620

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.