Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0005635

Physical Location & Seq

Genomic Coordinates Reverse (-)

9523542 .. 9527851

Gene Structure 4310 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g16670.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1257 bp

ATGGCCTCAGTTGGGTTCTGTCTCAGCTCTCTGATTCTATGTCCTACTTTCAGTTCCACTCAGACCTCAAAAAGACTGAGACCCTGTTCGAGAATCAGTCCAACAATGGCTCTGGCCACCCCAGCCACATTTGGGTTCAGTAACTTACTCGAGACTTTCACGGTCAACGTTCAGAGAGCAGAGAACAGGCCCCTGAATGTGCCTTTGATCGCTCCTTTCACGATTGCGACTTCGAGGCTTGAACGTGTTGAGAATGTTGCCATCAGAGTTGAGTTGAGTAATGGGTGTGTGGGGTGGGGTGAGGCTCCAATCTTGCCTCATGTGACTGCAGAGGATCAGGACACTGCTATGGTTAAGGCCAAGGAGGCTTGTGAGTTCTTGCTCAGGAATAATGGGATGACTTTGAGTTCGGTTTTGGGTGAGATTGGTGTGCTTCTTCCTGGATATGAGTTTGCTTCTGTTAGGGCTGGAGTCGAGATGGCTGTAATTGATGCGGTTGCTACAAGTATTGGTGTCCCTTTGTGGAGACTGTTTGGTGGAGCTTCAAATACCATAACCACAGATATAACGATTCCGATAGTTTCTGCAGATGAAGCTGCTAAACTGGCTTCAAAGTATTGCAAAGAAGGCTTTAAGACTTTAAAGCTTAAGGTGGGAAAGAATCTAAATGCAGACATAGAGGTCCTTCAAGCTATACGTGCTGTCCACCCTGATTGCTCTTTCATCTTGGATGCTAACGAAGGATATACCTCCAATGAAGCTATCCAAGTACTTGAGAAATTATATGAGGTAGGAGTGAGTCCAGTTCTCTTTGAACAGCCTGTTCATAGAGATGATTGGGAAGGCCTTGGATATGTTACTCGTATTGCTAAAGAAAGATATGGAGTATCAGTTGCAGCTGATGAGAGCTGTCGAAGTATAGTTGATGTTAAGAAAATAGCAGAACAAAGTCTTGCTAATGTCATTAACATAAAACTTACTAAAGTTGGGGTTGTTGGGGCCCTTGAAATCATTGAGGTTGCAAGAGCATCAGGATTGGATTTGATGATTGGTGGTATGGTTGAGACTAGACTCGCCATGGGTTTTTCTGGCCACCTTGCTGCTGGTCTTGGGTGCTTTAAGTTTATTGATCTAGATACGCCCCTATTGTTGTCAGAAGATCCAGTTCTGGAGGGCTATGAAGTTTCGGGTTCTGTTTATAAGTTCAACAATGCTAGAGGCCATGGTGGATTTCTCCACTGGGACAACCTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016853 GO:0016854 GO:0016855 GO:0034641 GO:0043167 GO:0043169 GO:0043603 GO:0044237 GO:0046872 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MR_MLE_C MR_MLE_N

Protein Analysis

419

Amino Acids

44.97

Weight (kDa)

5.26

Isoelectric Point (pI)

28.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MR_MLE_N	PF02746	68 - 179	1e-06	Mandelate racemase / muconate lactonizing enzyme, N-terminal domain
MR_MLE_C	PF13378	199 - 409	1.1e-33	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005635)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18270
fragaria_vesca	FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670
malus_domestica	MD06G1220000.v1.1 MD14G1229600.v1.1
prunus_persica	Prupe.5G224400_v2.0.a1 Prupe.5G224400_v2.0.a1
pyrus_communis	pycom14g19100 pycom14g19110
rosa_chinensis	RchiOBHm_Chr7g0179451
rosa_laevigata	RLG00000005355
rosa_multiflora	Rmu_co8470985.1_g000001
rosa_roxburghii	Rroxscaffold_3G00274010
rosa_rugosa	Rorug06G0424300 Rorug06G0424400 Rorug06G0424500 Rorug06G0424600
rosa_samantha	Rh2AG318700 Rh6AG144500 Rh7BG023500
rosa_wichuraiana	Rw4G009460 Rw4G033460 Rw5G048300 Rw7G001940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1200
AasI	GACNNNNNNGTC	1 cut(s)	680
AciI	CCGC	1 cut(s)	494
AclI	AACGTT	1 cut(s)	168
AclWI	GGATC	2 cut(s)	342, 1154
AcoI	YGGCCR	2 cut(s)	114, 1090
AfaI	GTAC	1 cut(s)	771
AfiI	CCNNNNNNNGG	2 cut(s)	12, 132
AflII	CTTAAG	1 cut(s)	647
AflIII	ACRYGT	1 cut(s)	244
AgsI	TTSAA	7 cut(s)	242, 546, 612, 689, 815, 1007, 1207
AjnI	CCWGG	1 cut(s)	439
AluBI	AGCT	8 cut(s)	27, 542, 596, 646, 692, 761, 899, 909
AluI	AGCT	8 cut(s)	27, 542, 596, 646, 692, 761, 899, 909
Alw26I	GTCTC	5 cut(s)	26, 73, 146, 520, 1058
AlwI	GGATC	2 cut(s)	342, 1154
Ama87I	CYCGRG	1 cut(s)	149
AoxI	GGCC	8 cut(s)	3, 114, 188, 357, 844, 999, 1090, 1219
ApaI	GGGCCC	1 cut(s)	1003
ApeKI	GCWGC	3 cut(s)	596, 896, 1100
ArsI	GACNNNNNNTTYG	2 cut(s)	391, 423
AspS9I	GGNCC	4 cut(s)	189, 682, 999, 1000
AsuHPI	GGTGA	2 cut(s)	311, 431
AvaI	CYCGRG	1 cut(s)	149
AvaII	GGWCC	1 cut(s)	682
BaeGI	GKGCMC	1 cut(s)	1003
BalI	TGGCCA	2 cut(s)	116, 1092
BanII	GRGCYC	1 cut(s)	1003
BbvI	GCAGC	3 cut(s)	583, 908, 1087
BccI	CCATC	2 cut(s)	269, 472
BciT130I	CCWGG	1 cut(s)	441
BcoDI	GTCTC	5 cut(s)	26, 73, 146, 520, 1058
BfaI	CTAG	3 cut(s)	1068, 1133, 1215
BfmI	CTRYAG	2 cut(s)	327, 585
BfrI	CTTAAG	1 cut(s)	647
BglI	GCCNNNNNGGC	1 cut(s)	365
BisI	GCNGC	3 cut(s)	597, 897, 1101
BlsI	GCNGC	3 cut(s)	598, 898, 1102
BmcAI	AGTACT	1 cut(s)	771
Bme1390I	CCNGG	1 cut(s)	441
Bme18I	GGWCC	1 cut(s)	682
BmeT110I	CYCGRG	1 cut(s)	149
BmgT120I	GGNCC	4 cut(s)	189, 682, 999, 1000
BmiI	GGNNCC	4 cut(s)	191, 306, 1000, 1001
BmrFI	CCNGG	1 cut(s)	441
BmrI	ACTGGG	1 cut(s)	1249
BmsI	GCATC	3 cut(s)	481, 721, 1037
BmuI	ACTGGG	1 cut(s)	1249
BpmI	CTGGAG	2 cut(s)	489, 1190
Bpu10I	CCTNAGC	1 cut(s)	383
BpuEI	CTTGAG	1 cut(s)	794
BsaAI	YACGTR	1 cut(s)	698
BsaI	GGTCTC	1 cut(s)	73
BsaJI	CCNNGG	4 cut(s)	360, 847, 1077, 1222
BsaXI	ACNNNNNCTCC	2 cut(s)	876, 906
Bsc4I	CCNNNNNNNGG	2 cut(s)	12, 132
Bse1I	ACTGG	4 cut(s)	609, 803, 1163, 1244
BseBI	CCWGG	1 cut(s)	441
BseDI	CCNNGG	4 cut(s)	360, 847, 1077, 1222
BseGI	GGATG	2 cut(s)	402, 736
BseLI	CCNNNNNNNGG	2 cut(s)	12, 132
BseMII	CTCAG	5 cut(s)	21, 37, 68, 74, 397
BseNI	ACTGG	4 cut(s)	609, 803, 1163, 1244
BseSI	GKGCMC	1 cut(s)	1003
BseXI	GCAGC	3 cut(s)	583, 908, 1087
BseYI	CCCAGC	1 cut(s)	121
BshFI	GGCC	8 cut(s)	5, 116, 190, 359, 846, 1001, 1092, 1221
BsiHKCI	CYCGRG	1 cut(s)	149
BslFI	GGGAC	1 cut(s)	500
BslI	CCNNNNNNNGG	2 cut(s)	12, 132
BsmAI	GTCTC	5 cut(s)	26, 73, 146, 520, 1058
BsmFI	GGGAC	1 cut(s)	500
BsnI	GGCC	8 cut(s)	5, 116, 190, 359, 846, 1001, 1092, 1221
Bso31I	GGTCTC	1 cut(s)	73
BsoBI	CYCGRG	1 cut(s)	149
Bsp120I	GGGCCC	1 cut(s)	999
Bsp1286I	GDGCHC	1 cut(s)	1003
Bsp143I	GATC	4 cut(s)	207, 334, 1129, 1159
Bsp19I	CCATGG	2 cut(s)	1077, 1222
BspACI	CCGC	1 cut(s)	494
BspANI	GGCC	8 cut(s)	5, 116, 190, 359, 846, 1001, 1092, 1221
BspCNI	CTCAG	5 cut(s)	20, 36, 69, 73, 396
BspLI	GGNNCC	4 cut(s)	191, 306, 1000, 1001
BspMAI	CTGCAG	2 cut(s)	331, 589
BspPI	GGATC	2 cut(s)	342, 1154
BspTI	CTTAAG	1 cut(s)	647
BspTNI	GGTCTC	1 cut(s)	73
BsrI	ACTGG	4 cut(s)	609, 803, 1163, 1244
BssECI	CCNNGG	4 cut(s)	360, 847, 1077, 1222
BssMI	GATC	4 cut(s)	207, 334, 1129, 1159
BssT1I	CCWWGG	4 cut(s)	360, 847, 1077, 1222
Bst2UI	CCWGG	1 cut(s)	441
Bst4CI	ACNGT	2 cut(s)	163, 531
BstAFI	CTTAAG	1 cut(s)	647
BstBAI	YACGTR	1 cut(s)	698
BstDEI	CTNAG	5 cut(s)	7, 23, 60, 77, 383
BstDSI	CCRYGG	2 cut(s)	1077, 1222
BstF5I	GGATG	2 cut(s)	402, 736
BstKTI	GATC	4 cut(s)	210, 337, 1132, 1162
BstMAI	GTCTC	5 cut(s)	26, 73, 146, 520, 1058
BstMBI	GATC	4 cut(s)	207, 334, 1129, 1159
BstMWI	GCNNNNNNNGC	6 cut(s)	122, 365, 593, 605, 627, 698
BstNI	CCWGG	1 cut(s)	441
BstSCI	CCNGG	1 cut(s)	439
BstSFI	CTRYAG	2 cut(s)	327, 585
BstSLI	GKGCMC	1 cut(s)	1003
BstV1I	GCAGC	3 cut(s)	583, 908, 1087
BstX2I	RGATCY	1 cut(s)	1159
BstYI	RGATCY	1 cut(s)	1159
BsuRI	GGCC	8 cut(s)	5, 116, 190, 359, 846, 1001, 1092, 1221
BtgI	CCRYGG	2 cut(s)	1077, 1222
BtsCI	GGATG	2 cut(s)	402, 736
BtsI	GCAGTG	1 cut(s)	342
BtsIMutI	CAGTG	2 cut(s)	342, 1237
Cfr13I	GGNCC	4 cut(s)	189, 682, 999, 1000
Csp6I	GTAC	1 cut(s)	770
CspCI	CAANNNNNGTGG	2 cut(s)	695, 730
CviAII	CATG	3 cut(s)	320, 1078, 1223
CviQI	GTAC	1 cut(s)	770
DdeI	CTNAG	5 cut(s)	7, 23, 60, 77, 383
DpnI	GATC	4 cut(s)	209, 336, 1131, 1161
DpnII	GATC	4 cut(s)	207, 334, 1129, 1159
DraI	TTTAAA	1 cut(s)	642
DrdI	GACNNNNNNGTC	1 cut(s)	680
DseDI	GACNNNNNNGTC	1 cut(s)	680
EaeI	YGGCCR	2 cut(s)	114, 1090
Eco130I	CCWWGG	4 cut(s)	360, 847, 1077, 1222
Eco147I	AGGCCT	1 cut(s)	846
Eco24I	GRGCYC	1 cut(s)	1003
Eco31I	GGTCTC	1 cut(s)	73
Eco47I	GGWCC	1 cut(s)	682
Eco88I	CYCGRG	1 cut(s)	149
EcoO109I	RGGNCCY	4 cut(s)	189, 682, 999, 1000
EcoRII	CCWGG	1 cut(s)	439
EcoT14I	CCWWGG	4 cut(s)	360, 847, 1077, 1222
EcoT38I	GRGCYC	1 cut(s)	1003
ErhI	CCWWGG	4 cut(s)	360, 847, 1077, 1222
FaeI	CATG	3 cut(s)	323, 1081, 1226
FaqI	GGGAC	1 cut(s)	500
FatI	CATG	3 cut(s)	319, 1077, 1222
Fnu4HI	GCNGC	3 cut(s)	597, 897, 1101
FokI	GGATG	2 cut(s)	409, 743
FriOI	GRGCYC	1 cut(s)	1003
Fsp4HI	GCNGC	3 cut(s)	597, 897, 1101
FspBI	CTAG	3 cut(s)	1068, 1133, 1215
GluI	GCNGC	3 cut(s)	597, 897, 1101
GsaI	CCCAGC	1 cut(s)	125
GsuI	CTGGAG	2 cut(s)	489, 1190
HaeIII	GGCC	8 cut(s)	5, 116, 190, 359, 846, 1001, 1092, 1221
Hin1II	CATG	3 cut(s)	323, 1081, 1226
HincII	GTYRAC	1 cut(s)	166
HindII	GTYRAC	1 cut(s)	166
HindIII	AAGCTT	1 cut(s)	644
HinfI	GANTC	7 cut(s)	34, 93, 471, 571, 661, 799, 1071
HphI	GGTGA	2 cut(s)	311, 431
Hpy166II	GTNNAC	2 cut(s)	166, 706
Hpy188I	TCNGA	6 cut(s)	33, 63, 174, 266, 576, 1156
Hpy188III	TCNNGA	9 cut(s)	90, 151, 220, 338, 385, 475, 1032, 1133, 1169
Hpy8I	GTNNAC	2 cut(s)	166, 706
HpyAV	CCTTC	4 cut(s)	620, 695, 734, 836
HpyCH4III	ACNGT	2 cut(s)	163, 531
HpyCH4IV	ACGT	3 cut(s)	168, 244, 697
HpyCH4V	TGCA	6 cut(s)	329, 587, 621, 671, 896, 1022
HpyF10VI	GCNNNNNNNGC	6 cut(s)	122, 365, 593, 605, 627, 698
HpyF3I	CTNAG	5 cut(s)	7, 23, 60, 77, 383
HpySE526I	ACGT	3 cut(s)	168, 244, 697
Hsp92II	CATG	3 cut(s)	323, 1081, 1226
Kzo9I	GATC	4 cut(s)	207, 334, 1129, 1159
LmnI	GCTCC	3 cut(s)	217, 310, 539
Lsp1109I	GCAGC	3 cut(s)	583, 908, 1087
LweI	GCATC	3 cut(s)	481, 721, 1037
MaeI	CTAG	3 cut(s)	1068, 1133, 1215
MaeII	ACGT	3 cut(s)	168, 244, 697
MaeIII	GTNAC	3 cut(s)	140, 322, 856
MalI	GATC	4 cut(s)	209, 336, 1131, 1161
MboI	GATC	4 cut(s)	207, 334, 1129, 1159
MboII	GAAGA	2 cut(s)	428, 1169
MflI	RGATCY	1 cut(s)	1159
MhlI	GDGCHC	1 cut(s)	1003
MlsI	TGGCCA	2 cut(s)	116, 1092
MluCI	AATT	2 cut(s)	486, 779
MluNI	TGGCCA	2 cut(s)	116, 1092
MlyI	GAGTC	3 cut(s)	480, 808, 1065
MmeI	TCCRAC	1 cut(s)	125
Mox20I	TGGCCA	2 cut(s)	116, 1092
MscI	TGGCCA	2 cut(s)	116, 1092
MseI	TTAA	7 cut(s)	354, 633, 641, 648, 930, 966, 1119
MslI	CAYNNNNRTG	2 cut(s)	347, 831
Msp20I	TGGCCA	2 cut(s)	116, 1092
MspA1I	CMGCKG	1 cut(s)	899
MspCI	CTTAAG	1 cut(s)	647
MspR9I	CCNGG	1 cut(s)	441
MvaI	CCWGG	1 cut(s)	441
MwoI	GCNNNNNNNGC	6 cut(s)	122, 365, 593, 605, 627, 698
NcoI	CCATGG	2 cut(s)	1077, 1222
NdeII	GATC	4 cut(s)	207, 334, 1129, 1159
NlaIII	CATG	3 cut(s)	323, 1081, 1226
NlaIV	GGNNCC	4 cut(s)	191, 306, 1000, 1001
NmuCI	GTSAC	1 cut(s)	322
PaeR7I	CTCGAG	1 cut(s)	149
PceI	AGGCCT	1 cut(s)	846
PfeI	GAWTC	4 cut(s)	34, 93, 571, 661
PfoI	TCCNGGA	1 cut(s)	439
PkrI	GCNGC	3 cut(s)	598, 898, 1102
PleI	GAGTC	3 cut(s)	479, 807, 1065
PpsI	GAGTC	3 cut(s)	479, 807, 1065
Ppu21I	YACGTR	1 cut(s)	698
PpuMI	RGGWCCY	1 cut(s)	682
PsiI	TTATAA	1 cut(s)	1200
Psp1406I	AACGTT	1 cut(s)	168
Psp5II	RGGWCCY	1 cut(s)	682
Psp6I	CCWGG	1 cut(s)	439
PspFI	CCCAGC	1 cut(s)	121
PspGI	CCWGG	1 cut(s)	439
PspN4I	GGNNCC	4 cut(s)	191, 306, 1000, 1001
PspOMI	GGGCCC	1 cut(s)	999
PspPI	GGNCC	4 cut(s)	189, 682, 999, 1000
PspPPI	RGGWCCY	1 cut(s)	682
PstI	CTGCAG	2 cut(s)	331, 589
PsuI	RGATCY	1 cut(s)	1159
PvuII	CAGCTG	1 cut(s)	899
RsaI	GTAC	1 cut(s)	771
RsaNI	GTAC	1 cut(s)	770
RseI	CAYNNNNRTG	2 cut(s)	347, 831
SaqAI	TTAA	7 cut(s)	354, 633, 641, 648, 930, 966, 1119
SatI	GCNGC	3 cut(s)	597, 897, 1101
Sau3AI	GATC	4 cut(s)	207, 334, 1129, 1159
Sau96I	GGNCC	4 cut(s)	189, 682, 999, 1000
ScaI	AGTACT	1 cut(s)	771
SchI	GAGTC	3 cut(s)	480, 808, 1065
ScrFI	CCNGG	1 cut(s)	441
SduI	GDGCHC	1 cut(s)	1003
SfaNI	GCATC	3 cut(s)	481, 721, 1037
SfcI	CTRYAG	2 cut(s)	327, 585
Sfr274I	CTCGAG	1 cut(s)	149
SinI	GGWCC	1 cut(s)	682
SlaI	CTCGAG	1 cut(s)	149
SmiMI	CAYNNNNRTG	2 cut(s)	347, 831
SmlI	CTYRAG	3 cut(s)	149, 647, 773
SmoI	CTYRAG	3 cut(s)	149, 647, 773
Sse9I	AATT	2 cut(s)	486, 779
SseBI	AGGCCT	1 cut(s)	846
SsiI	CCGC	1 cut(s)	494
SspMI	CTAG	3 cut(s)	1068, 1133, 1215
StuI	AGGCCT	1 cut(s)	846
StyD4I	CCNGG	1 cut(s)	439
StyI	CCWWGG	4 cut(s)	360, 847, 1077, 1222
TaaI	ACNGT	2 cut(s)	163, 531
TaiI	ACGT	3 cut(s)	171, 247, 700
TaqI	TCGA	5 cut(s)	89, 150, 233, 474, 913
TasI	AATT	2 cut(s)	486, 779
TatI	WGTACW	1 cut(s)	769
TfiI	GAWTC	4 cut(s)	34, 93, 571, 661
Tru1I	TTAA	7 cut(s)	354, 633, 641, 648, 930, 966, 1119
Tru9I	TTAA	7 cut(s)	354, 633, 641, 648, 930, 966, 1119
TscAI	CASTG	2 cut(s)	349, 1244
TseFI	GTSAC	1 cut(s)	322
TseI	GCWGC	3 cut(s)	596, 896, 1100
Tsp45I	GTSAC	1 cut(s)	322
TspDTI	ATGAA	5 cut(s)	606, 712, 771, 815, 1194
TspRI	CASTG	2 cut(s)	349, 1244
Vha464I	CTTAAG	1 cut(s)	647
VpaK11BI	GGWCC	1 cut(s)	682
XbaI	TCTAGA	1 cut(s)	1132
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1100
XhoI	CTCGAG	1 cut(s)	149
XspI	CTAG	3 cut(s)	1068, 1133, 1215
ZrmI	AGTACT	1 cut(s)	771

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.