Rorug06G0424300

L-Ala-D L-amino acid

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005635
Physical Location & Seq
Forward (+)
56213261 .. 56216779
3519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0424300.1

Sequence Viewer

Length: 1065 bp
ATGAACAAACAGAGACGGCCACCGCCGGACCCAGTGGCTGTGCTTAGAGGTCACCGTGCCTCTGTTTCCATCCATCTCAACCTCTCCTATACACTGGACGGTGAGTTGCGGATTTGGGACACTCTGCAGAATCGGACGATAGTATCAGCAGGGGTGCATAGTGCAGCACATGGGGTTGCTTCTGTTGCTTGTAGCTCGTCGATTGGAGCTGAAAAAGTTATCAGCCAGGGTAGGGATGGAACTGTTAAGTGCTGGGATATTGGAGATGGAGGTCTATCCAGGACTCCCTCTCTGACGATCGCGACGAATGCTCACCACTTCTGTAAGCTTTCGTTGGTGAAGAGACCTCATTCTTGTTCTACACAAGTTGACGGGCCAAAGCATCATGACGGAGAAAATACTCAAGTTGGAGGATCTAAATACATTGCTATAGCAGGAGAGCAGTCTTCTGAGGTTGAGATTTGGGATCTTAATACTGCAGAAAGGTGTATGCGACTACCTCAAAGCTGTTCTGCTGGCTCCTCAGCTATTTCTACCAAGGAAAGAGGAATGTGCATGGCAGTTCAAGTATTTTTACCATTGGAATCACGGGGATTCCTAAATGTTGTGGCTGGTTATGAGGATGGCTCCATGCTTTGGTGGGATATTAGAAATCCAGGTGTTCCAGTTACATCTGTCAAGTTTCATTCAGAGCCAGTTTTAAGCCTATGCATTGATGGTTCATGCAATGGTGGTATCTCAGGAGCTGCAGATGAAAAAATTATGCTCTACAGTTTGGATAATTCCATGGGTACATGTGTGATAAAAAAAGAAATTGATTTGGAGCGACCTGGCATATCAAGCACCTCTATTCGACCTGATGGTAAAATATTTGCAACAGCTGGCTGGGATCACAGAGTGAGCGTATATAACTATCGCAAAGGAAATGCTTTGGCAATATTGAAGTACCACCATGCAACGTGTAATGCAGTCTCTTATTCAACAGATTGTAAGCTAATGGCCTCTGCATCAGAGGACACAGTGGTGGCACTTTGGAAACTCTATACTCCTCAAACCTGGGAGTGA

Protein Analysis

354

Amino Acids

38.32

Weight (kDa)

7.18

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 149 - 269 9.4e-06 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 190 - 346 1.5e-09 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 202 - 347 5e-16 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 202 - 351 3e-10 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 202 - 349 7.6e-07 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 203 - 348 6.2e-15 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 203 - 349 3.3e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 203 - 349 9.8e-08 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 222 - 349 2.6e-07 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 236 - 347 8.6e-09 MABP1/WDR62 second WD40 domain
Beta-prop_WDR5 PF25175 277 - 351 1.1e-13 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 279 - 349 3.7e-08 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 636
AccII CGCG 1 cut(s) 302
AciI CCGC 2 cut(s) 23, 109
AclWI GGATC 3 cut(s) 421, 474, 897
AcoI YGGCCR 1 cut(s) 17
AdeI CACNNNGTG 1 cut(s) 898
AfaI GTAC 2 cut(s) 793, 947
AfiI CCNNNNNNNGG 2 cut(s) 232, 636
AflIII ACRYGT 2 cut(s) 794, 959
AgsI TTSAA 3 cut(s) 566, 943, 981
AjnI CCWGG 5 cut(s) 225, 278, 655, 829, 1055
AleI CACNNNNGTG 1 cut(s) 1022
AluBI AGCT 8 cut(s) 195, 209, 328, 507, 527, 746, 881, 994
AluI AGCT 8 cut(s) 195, 209, 328, 507, 527, 746, 881, 994
Alw26I GTCTC 3 cut(s) 7, 337, 976
AlwI GGATC 3 cut(s) 421, 474, 897
AlwNI CAGNNNCTG 2 cut(s) 38, 746
AoxI GGCC 3 cut(s) 17, 374, 999
ApeKI GCWGC 2 cut(s) 164, 746
AspS9I GGNCC 2 cut(s) 28, 374
AsuHPI GGTGA 4 cut(s) 44, 113, 305, 349
AvaII GGWCC 1 cut(s) 28
BaeI ACNNNNGTAYC 4 cut(s) 126, 159, 783, 816
BbsI GAAGAC 1 cut(s) 438
BbvCI CCTCAGC 1 cut(s) 523
BbvI GCAGC 2 cut(s) 176, 733
BccI CCATC 7 cut(s) 77, 81, 230, 260, 617, 710, 854
BceAI ACGGC 1 cut(s) 32
BciT130I CCWGG 5 cut(s) 227, 280, 657, 831, 1057
BcoDI GTCTC 3 cut(s) 7, 337, 976
BfmI CTRYAG 5 cut(s) 125, 429, 477, 747, 769
BisI GCNGC 2 cut(s) 165, 747
BlsI GCNGC 2 cut(s) 166, 748
Bme1390I CCNGG 5 cut(s) 227, 280, 657, 831, 1057
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 2 cut(s) 28, 374
BmiI GGNNCC 3 cut(s) 30, 520, 628
BmrFI CCNGG 5 cut(s) 227, 280, 657, 831, 1057
BmrI ACTGGG 1 cut(s) 26
BmsI GCATC 2 cut(s) 391, 1016
BmuI ACTGGG 1 cut(s) 26
BpiI GAAGAC 1 cut(s) 438
BplI GAGNNNNNCTC 2 cut(s) 611, 643
Bpu10I CCTNAGC 1 cut(s) 523
BpuEI CTTGAG 1 cut(s) 387
BsaI GGTCTC 1 cut(s) 337
BsaJI CCNNGG 4 cut(s) 226, 537, 786, 1056
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 636
Bse1I ACTGG 4 cut(s) 32, 99, 665, 695
Bse3DI GCAATG 2 cut(s) 423, 733
BseBI CCWGG 5 cut(s) 227, 280, 657, 831, 1057
BseDI CCNNGG 4 cut(s) 226, 537, 786, 1056
BseGI GGATG 3 cut(s) 69, 241, 628
BseLI CCNNNNNNNGG 2 cut(s) 232, 636
BseMI GCAATG 2 cut(s) 423, 733
BseMII CTCAG 3 cut(s) 441, 537, 753
BseNI ACTGG 4 cut(s) 32, 99, 665, 695
BseRI GAGGAG 2 cut(s) 511, 1038
BseXI GCAGC 2 cut(s) 176, 733
BseYI CCCAGC 2 cut(s) 252, 885
BsgI GTGCAG 1 cut(s) 183
Bsh1236I CGCG 1 cut(s) 302
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 3 cut(s) 19, 376, 1001
BsiEI CGRYCG 1 cut(s) 300
BsiSI CCGG 1 cut(s) 26
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 232, 636
BsmAI GTCTC 3 cut(s) 7, 337, 976
BsmBI CGTCTC 1 cut(s) 7
BsmFI GGGAC 1 cut(s) 131
BsmI GAATGC 1 cut(s) 313
BsnI GGCC 3 cut(s) 19, 376, 1001
Bso31I GGTCTC 1 cut(s) 337
Bsp143I GATC 4 cut(s) 297, 413, 466, 889
Bsp19I CCATGG 1 cut(s) 786
Bsp68I TCGCGA 1 cut(s) 302
BspACI CCGC 2 cut(s) 23, 109
BspANI GGCC 3 cut(s) 19, 376, 1001
BspCNI CTCAG 3 cut(s) 442, 536, 752
BspFNI CGCG 1 cut(s) 302
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 3 cut(s) 30, 520, 628
BspMAI CTGCAG 3 cut(s) 129, 481, 751
BspPI GGATC 3 cut(s) 421, 474, 897
BspTNI GGTCTC 1 cut(s) 337
BsrDI GCAATG 2 cut(s) 423, 733
BsrI ACTGG 4 cut(s) 32, 99, 665, 695
BssECI CCNNGG 4 cut(s) 226, 537, 786, 1056
BssMI GATC 4 cut(s) 297, 413, 466, 889
BssT1I CCWWGG 2 cut(s) 537, 786
Bst2UI CCWGG 5 cut(s) 227, 280, 657, 831, 1057
Bst4CI ACNGT 5 cut(s) 56, 101, 244, 773, 1021
Bst6I CTCTTC 1 cut(s) 335
BstC8I GCNNGC 2 cut(s) 517, 883
BstDEI CTNAG 4 cut(s) 44, 450, 523, 739
BstDSI CCRYGG 1 cut(s) 786
BstEII GGTNACC 1 cut(s) 50
BstF5I GGATG 3 cut(s) 69, 241, 628
BstFNI CGCG 1 cut(s) 302
BstKTI GATC 4 cut(s) 300, 416, 469, 892
BstMAI GTCTC 3 cut(s) 7, 337, 976
BstMBI GATC 4 cut(s) 297, 413, 466, 889
BstMCI CGRYCG 1 cut(s) 300
BstMWI GCNNNNNNNGC 3 cut(s) 185, 308, 840
BstNI CCWGG 5 cut(s) 227, 280, 657, 831, 1057
BstNSI RCATGY 1 cut(s) 798
BstPI GGTNACC 1 cut(s) 50
BstSCI CCNGG 5 cut(s) 225, 278, 655, 829, 1055
BstSFI CTRYAG 5 cut(s) 125, 429, 477, 747, 769
BstUI CGCG 1 cut(s) 302
BstV1I GCAGC 2 cut(s) 176, 733
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 2 cut(s) 413, 466
BstYI RGATCY 2 cut(s) 413, 466
BsuRI GGCC 3 cut(s) 19, 376, 1001
BtgI CCRYGG 1 cut(s) 786
BtsCI GGATG 3 cut(s) 69, 241, 628
BtsIMutI CAGTG 3 cut(s) 39, 92, 1026
BtuMI TCGCGA 1 cut(s) 302
Cac8I GCNNGC 2 cut(s) 517, 883
CaiI CAGNNNCTG 2 cut(s) 38, 746
CciI TCATGA 1 cut(s) 385
Cfr13I GGNCC 2 cut(s) 28, 374
Csp6I GTAC 2 cut(s) 792, 946
CviAII CATG 8 cut(s) 170, 386, 556, 631, 723, 787, 795, 953
CviQI GTAC 2 cut(s) 792, 946
DdeI CTNAG 4 cut(s) 44, 450, 523, 739
DpnI GATC 4 cut(s) 299, 415, 468, 891
DpnII GATC 4 cut(s) 297, 413, 466, 889
DraIII CACNNNGTG 1 cut(s) 898
EaeI YGGCCR 1 cut(s) 17
Eam1104I CTCTTC 1 cut(s) 335
EarI CTCTTC 1 cut(s) 335
Eco130I CCWWGG 2 cut(s) 537, 786
Eco31I GGTCTC 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 28
Eco91I GGTNACC 1 cut(s) 50
EcoO65I GGTNACC 1 cut(s) 50
EcoRII CCWGG 5 cut(s) 225, 278, 655, 829, 1055
EcoT14I CCWWGG 2 cut(s) 537, 786
EcoT22I ATGCAT 1 cut(s) 713
ErhI CCWWGG 2 cut(s) 537, 786
Esp3I CGTCTC 1 cut(s) 7
FaeI CATG 8 cut(s) 173, 389, 559, 634, 726, 790, 798, 956
FaqI GGGAC 1 cut(s) 131
FatI CATG 8 cut(s) 169, 385, 555, 630, 722, 786, 794, 952
Fnu4HI GCNGC 2 cut(s) 165, 747
FokI GGATG 3 cut(s) 56, 248, 635
Fsp4HI GCNGC 2 cut(s) 165, 747
GluI GCNGC 2 cut(s) 165, 747
GsaI CCCAGC 2 cut(s) 256, 889
HaeIII GGCC 3 cut(s) 19, 376, 1001
HapII CCGG 1 cut(s) 26
Hin1II CATG 8 cut(s) 173, 389, 559, 634, 726, 790, 798, 956
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 4 cut(s) 130, 283, 584, 594
HpaII CCGG 1 cut(s) 26
HphI GGTGA 4 cut(s) 44, 113, 305, 349
Hpy166II GTNNAC 1 cut(s) 370
Hpy188I TCNGA 5 cut(s) 135, 294, 451, 691, 1012
Hpy188III TCNNGA 3 cut(s) 301, 386, 741
Hpy8I GTNNAC 1 cut(s) 370
Hpy99I CGWCG 2 cut(s) 202, 307
HpyCH4III ACNGT 5 cut(s) 56, 101, 244, 773, 1021
HpyCH4IV ACGT 1 cut(s) 959
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 308, 840
HpyF3I CTNAG 4 cut(s) 44, 450, 523, 739
HpySE526I ACGT 1 cut(s) 959
Hsp92II CATG 8 cut(s) 173, 389, 559, 634, 726, 790, 798, 956
Kzo9I GATC 4 cut(s) 297, 413, 466, 889
LmnI GCTCC 5 cut(s) 206, 524, 632, 743, 823
Lsp1109I GCAGC 2 cut(s) 176, 733
LweI GCATC 2 cut(s) 391, 1016
MaeII ACGT 1 cut(s) 959
MaeIII GTNAC 2 cut(s) 50, 667
MalI GATC 4 cut(s) 299, 415, 468, 891
MboI GATC 4 cut(s) 297, 413, 466, 889
MboII GAAGA 2 cut(s) 352, 438
MflI RGATCY 2 cut(s) 413, 466
MluCI AATT 3 cut(s) 759, 781, 813
MlyI GAGTC 1 cut(s) 277
MmeI TCCRAC 1 cut(s) 388
Mph1103I ATGCAT 1 cut(s) 713
MseI TTAA 3 cut(s) 246, 471, 701
MslI CAYNNNNRTG 1 cut(s) 1022
MspA1I CMGCKG 1 cut(s) 881
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 5 cut(s) 227, 280, 657, 831, 1057
Mva1269I GAATGC 1 cut(s) 313
MvaI CCWGG 5 cut(s) 227, 280, 657, 831, 1057
MvnI CGCG 1 cut(s) 302
MwoI GCNNNNNNNGC 3 cut(s) 185, 308, 840
NcoI CCATGG 1 cut(s) 786
NdeII GATC 4 cut(s) 297, 413, 466, 889
NlaIII CATG 8 cut(s) 173, 389, 559, 634, 726, 790, 798, 956
NlaIV GGNNCC 3 cut(s) 30, 520, 628
NmuCI GTSAC 1 cut(s) 50
NruI TCGCGA 1 cut(s) 302
NsiI ATGCAT 1 cut(s) 713
NspI RCATGY 1 cut(s) 798
OliI CACNNNNGTG 1 cut(s) 1022
PagI TCATGA 1 cut(s) 385
PciI ACATGT 1 cut(s) 794
PcsI WCGNNNNNNNCGW 1 cut(s) 302
PctI GAATGC 1 cut(s) 313
PfeI GAWTC 3 cut(s) 130, 584, 594
PflMI CCANNNNNTGG 1 cut(s) 636
PfoI TCCNGGA 1 cut(s) 278
PkrI GCNGC 2 cut(s) 166, 748
Ple19I CGATCG 1 cut(s) 300
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PscI ACATGT 1 cut(s) 794
Psp6I CCWGG 5 cut(s) 225, 278, 655, 829, 1055
PspEI GGTNACC 1 cut(s) 50
PspFI CCCAGC 2 cut(s) 252, 885
PspGI CCWGG 5 cut(s) 225, 278, 655, 829, 1055
PspN4I GGNNCC 3 cut(s) 30, 520, 628
PspPI GGNCC 2 cut(s) 28, 374
PstI CTGCAG 3 cut(s) 129, 481, 751
PstNI CAGNNNCTG 2 cut(s) 38, 746
PsuI RGATCY 2 cut(s) 413, 466
PvuI CGATCG 1 cut(s) 300
PvuII CAGCTG 1 cut(s) 881
RruI TCGCGA 1 cut(s) 302
RsaI GTAC 2 cut(s) 793, 947
RsaNI GTAC 2 cut(s) 792, 946
RseI CAYNNNNRTG 1 cut(s) 1022
SaqAI TTAA 3 cut(s) 246, 471, 701
SatI GCNGC 2 cut(s) 165, 747
Sau3AI GATC 4 cut(s) 297, 413, 466, 889
Sau96I GGNCC 2 cut(s) 28, 374
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 5 cut(s) 227, 280, 657, 831, 1057
SfaNI GCATC 2 cut(s) 391, 1016
SfcI CTRYAG 5 cut(s) 125, 429, 477, 747, 769
SinI GGWCC 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 1022
SmlI CTYRAG 1 cut(s) 402
SmoI CTYRAG 1 cut(s) 402
Sse9I AATT 3 cut(s) 759, 781, 813
SsiI CCGC 2 cut(s) 23, 109
SspI AATATT 2 cut(s) 870, 939
StyD4I CCNGG 5 cut(s) 225, 278, 655, 829, 1055
StyI CCWWGG 2 cut(s) 537, 786
TaaI ACNGT 5 cut(s) 56, 101, 244, 773, 1021
TaiI ACGT 1 cut(s) 962
TaqI TCGA 2 cut(s) 200, 853
TasI AATT 3 cut(s) 759, 781, 813
TfiI GAWTC 3 cut(s) 130, 584, 594
Tru1I TTAA 3 cut(s) 246, 471, 701
Tru9I TTAA 3 cut(s) 246, 471, 701
TscAI CASTG 3 cut(s) 39, 99, 1026
TseFI GTSAC 1 cut(s) 50
TseI GCWGC 2 cut(s) 164, 746
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 4 cut(s) 17, 674, 711, 768
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 3 cut(s) 39, 99, 1026
Van91I CCANNNNNTGG 1 cut(s) 636
VpaK11BI GGWCC 1 cut(s) 28
XceI RCATGY 1 cut(s) 798
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
Zsp2I ATGCAT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.