Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005635

Physical Location & Seq

Genomic Coordinates Reverse (-)

31071762 .. 31075204

Gene Structure 3443 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1229600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1443 bp

ATGAGCTCAATTGGGTTTTCTCTTCGCTCACCGTTCAGCTCTCTGCTCCTCTGCTCTGCTCCTCATTCCACTCGAAACCCCAAAAAACCAAAACCCTTTTCAAAATTCACCCCAAAAATGGCTCTGGCCACCCCTACCAGTTTTGGGTTCAGCAGTCTGCTCGAGACGTTCACGGTGAACGTGCAGAGAGCAGAGAATCGGCCTCTGAACGTGCCGCTGACTGCGCCGTTCACGATTGCGACTTCGCGGCTGGAGAGGGTGGAGAATGTGGCGGTCCGGGTTGAGTTGAGTAACGGGTGCGTGGGGTGGGGTGAAACTCCGATTTTGCCCTTTGTGACTGCAGAGGATCAGCATACTGCCATGGTTAAGGCTAAGGAAGCTTGCGAGTTCTTGCTTCGAAGTCCGGCGATGACGTTGGGTTCGGTTTTGGGGGAGATTGGTGGGATTCTTCCTGGACATGAGTTTGCTTCTGTTAGGGCAGGAGTTGAGATGGCGTTAATTGATGCAGTTTGTATGAGTATCGGTGTCCCTTTGTGGAGACTGTTTGGTGGAGCTTCAAACACCATAACTACTGATATAACAATTCCGATTGTTTCTGCAGCAGAAGCTGCTACATTGGCTTCAAAGTACCGTGAAGAAGGGTTTAAAACTTTAAAGCTTAAGGTGGGAAAGAATTTGAATTCAGACATAGAAGTCCTTCGAGCCATACGTGCTGTTCACCCCGATTGCTATTTTATCTTGGATGCTAACGAAGGATATAACTCCAACGAAGCTATCCAAGTTCTTGATAAATTACATGAGGTGGGAGTGAGTCCTCTTCTCTTCGAACAACCGGTCCATAGAGATGATTGGAAAGGCCTCGGAAATGTTTACCACGTTGCGAAAGACAAATATGGAGTATCAGTTGCAGCTGATGAGAGCTGTCGAAGTTTAGCTGATGTTAAGAAAATAGTGGAAGAAAATCTTGCCGATGTAATTAACATTAAACTTGCTAAAGTTGGGGTTGTTCGAGCCCTTGAAATCATCGAGGTAGCAAAGGCAGCGGGATTGAATTTGATGATCGGTGGTATGGTTGAGACTAGACTGGCCATGGGTTTTGCTGGCCATCTTGCTGCTGGCATGGGGTGCTTTAAGTTTGTTGACCTAGACACGCCCTTGCTGCTATCAGAAGATCCGGTTCTGGAGGGTTACGAAGTTTCGGGTGCGGTTTATAAGTTCAGAAATGCTAGAGGCCATGGTGGATTTCTGCACTGGGACAATATTGCTTGCTTGCAATTCAACATTTTAAACTCCGAGCAGCATCTGATGGCGGAAAAAAAGCTCCCCAGGATGGAGCTTTTTCAAATGCTGTCGTATTTTTTTTCAGATGAAACATTTGAATTCATAGACCACAACAGCATTCGGCATTGCAAACGCATCGTAAATAATTGCTTAGCTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016853 GO:0016854 GO:0016855 GO:0034641 GO:0043167 GO:0043169 GO:0043603 GO:0044237 GO:0046872 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MR_MLE_C MR_MLE_N

Protein Analysis

481

Amino Acids

52.56

Weight (kDa)

6.14

Isoelectric Point (pI)

37.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MR_MLE_N	PF02746	71 - 183	1.6e-06	Mandelate racemase / muconate lactonizing enzyme, N-terminal domain
MR_MLE_C	PF13378	204 - 413	9.1e-35	Enolase C-terminal domain-like
MAAL_C	PF07476	238 - 362	7e-08	Methylaspartate ammonia-lyase C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005635)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18270
fragaria_vesca	FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670
malus_domestica	MD06G1220000.v1.1 MD14G1229600.v1.1
prunus_persica	Prupe.5G224400_v2.0.a1 Prupe.5G224400_v2.0.a1
pyrus_communis	pycom14g19100 pycom14g19110
rosa_chinensis	RchiOBHm_Chr7g0179451
rosa_laevigata	RLG00000005355
rosa_multiflora	Rmu_co8470985.1_g000001
rosa_roxburghii	Rroxscaffold_3G00274010
rosa_rugosa	Rorug06G0424300 Rorug06G0424400 Rorug06G0424500 Rorug06G0424600
rosa_samantha	Rh2AG318700 Rh6AG144500 Rh7BG023500
rosa_wichuraiana	Rw4G009460 Rw4G033460 Rw5G048300 Rw7G001940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1212
AasI	GACNNNNNNGTC	1 cut(s)	692
AccII	CGCG	1 cut(s)	247
AciI	CCGC	6 cut(s)	215, 247, 272, 1043, 1205, 1310
AclWI	GGATC	2 cut(s)	354, 1166
AcoI	YGGCCR	3 cut(s)	126, 1086, 1102
AcsI	RAATTY	5 cut(s)	104, 673, 679, 1051, 1379
AfaI	GTAC	1 cut(s)	629
AfiI	CCNNNNNNNGG	3 cut(s)	118, 144, 1330
AflII	CTTAAG	1 cut(s)	659
AgeI	ACCGGT	1 cut(s)	832
AgsI	TTSAA	9 cut(s)	102, 558, 624, 679, 1019, 1051, 1279, 1343, 1379
AjnI	CCWGG	2 cut(s)	451, 1325
AjuI	GAANNNNNNNTTGG	2 cut(s)	82, 114
AloI	GAACNNNNNNTCC	2 cut(s)	819, 851
Alw21I	GWGCWC	1 cut(s)	8
Alw26I	GTCTC	3 cut(s)	158, 532, 1070
AlwI	GGATC	2 cut(s)	354, 1166
AlwNI	CAGNNNCTG	2 cut(s)	608, 1303
Ama87I	CYCGRG	1 cut(s)	161
AoxI	GGCC	6 cut(s)	126, 200, 856, 1086, 1102, 1231
ApeKI	GCWGC	7 cut(s)	599, 608, 908, 1040, 1112, 1159, 1297
ApoI	RAATTY	5 cut(s)	104, 673, 679, 1051, 1379
ArsI	GACNNNNNNTTYG	4 cut(s)	403, 435, 819, 851
AsiGI	ACCGGT	1 cut(s)	832
Asp700I	GAANNNNTTC	1 cut(s)	696
AspLEI	GCGC	1 cut(s)	226
AspS9I	GGNCC	2 cut(s)	274, 835
AsuC2I	CCSGG	1 cut(s)	278
AsuHPI	GGTGA	5 cut(s)	21, 100, 187, 323, 710
AsuII	TTCGAA	2 cut(s)	397, 825
AvaI	CYCGRG	1 cut(s)	161
AvaII	GGWCC	2 cut(s)	274, 835
BalI	TGGCCA	3 cut(s)	128, 1088, 1104
BanII	GRGCYC	2 cut(s)	8, 1015
Bbv12I	GWGCWC	1 cut(s)	8
BbvI	GCAGC	7 cut(s)	595, 611, 920, 1052, 1099, 1146, 1309
BccI	CCATC	4 cut(s)	484, 1113, 1300, 1324
BceAI	ACGGC	1 cut(s)	211
BcgI	CGANNNNNNTGC	4 cut(s)	142, 176, 1399, 1433
BciT130I	CCWGG	2 cut(s)	453, 1327
BcnI	CCSGG	1 cut(s)	278
BcoDI	GTCTC	3 cut(s)	158, 532, 1070
BfaI	CTAG	3 cut(s)	1080, 1145, 1227
BfmI	CTRYAG	2 cut(s)	339, 597
BfrI	CTTAAG	1 cut(s)	659
BisI	GCNGC	9 cut(s)	215, 248, 600, 609, 909, 1041, 1113, 1160, 1298
BlpI	GCTNAGC	1 cut(s)	1432
BlsI	GCNGC	9 cut(s)	216, 249, 601, 610, 910, 1042, 1114, 1161, 1299
Bme1390I	CCNGG	3 cut(s)	278, 453, 1327
Bme18I	GGWCC	2 cut(s)	274, 835
BmeT110I	CYCGRG	1 cut(s)	161
BmgT120I	GGNCC	2 cut(s)	274, 835
BmrFI	CCNGG	3 cut(s)	278, 453, 1327
BmrI	ACTGGG	1 cut(s)	1261
BmsI	GCATC	4 cut(s)	493, 733, 1309, 1425
BmuI	ACTGGG	1 cut(s)	1261
BpmI	CTGGAG	2 cut(s)	272, 1202
Bpu10I	CCTNAGC	1 cut(s)	372
Bpu1102I	GCTNAGC	1 cut(s)	1432
Bpu14I	TTCGAA	2 cut(s)	397, 825
BpuMI	CCSGG	1 cut(s)	278
BsaAI	YACGTR	1 cut(s)	710
BsaJI	CCNNGG	5 cut(s)	360, 859, 1089, 1234, 1325
BsaWI	WCCGGW	2 cut(s)	832, 1174
BsaXI	ACNNNNNCTCC	2 cut(s)	888, 918
Bsc4I	CCNNNNNNNGG	3 cut(s)	118, 144, 1330
Bse118I	RCCGGY	1 cut(s)	832
Bse1I	ACTGG	3 cut(s)	138, 1089, 1256
Bse3DI	GCAATG	1 cut(s)	1405
BseBI	CCWGG	2 cut(s)	453, 1327
BseDI	CCNNGG	5 cut(s)	360, 859, 1089, 1234, 1325
BseGI	GGATG	2 cut(s)	748, 1335
BseLI	CCNNNNNNNGG	3 cut(s)	118, 144, 1330
BseMI	GCAATG	1 cut(s)	1405
BseNI	ACTGG	3 cut(s)	138, 1089, 1256
BseRI	GAGGAG	2 cut(s)	38, 51
BseXI	GCAGC	7 cut(s)	595, 611, 920, 1052, 1099, 1146, 1309
BsgI	GTGCAG	2 cut(s)	203, 1232
Bsh1236I	CGCG	1 cut(s)	247
BshFI	GGCC	6 cut(s)	128, 202, 858, 1088, 1104, 1233
BshTI	ACCGGT	1 cut(s)	832
BsiHKAI	GWGCWC	1 cut(s)	8
BsiHKCI	CYCGRG	1 cut(s)	161
BsiSI	CCGG	4 cut(s)	277, 404, 833, 1175
BslFI	GGGAC	2 cut(s)	512, 1268
BslI	CCNNNNNNNGG	3 cut(s)	118, 144, 1330
BsmAI	GTCTC	3 cut(s)	158, 532, 1070
BsmBI	CGTCTC	1 cut(s)	158
BsmFI	GGGAC	2 cut(s)	512, 1268
BsmI	GAATGC	1 cut(s)	1398
BsnI	GGCC	6 cut(s)	128, 202, 858, 1088, 1104, 1233
BsoBI	CYCGRG	1 cut(s)	161
Bsp119I	TTCGAA	2 cut(s)	397, 825
Bsp1286I	GDGCHC	2 cut(s)	8, 1015
Bsp143I	GATC	3 cut(s)	346, 1059, 1171
Bsp1720I	GCTNAGC	1 cut(s)	1432
Bsp19I	CCATGG	3 cut(s)	360, 1089, 1234
BspACI	CCGC	6 cut(s)	215, 247, 272, 1043, 1205, 1310
BspANI	GGCC	6 cut(s)	128, 202, 858, 1088, 1104, 1233
BspFNI	CGCG	1 cut(s)	247
BspMAI	CTGCAG	2 cut(s)	343, 601
BspPI	GGATC	2 cut(s)	354, 1166
BspT104I	TTCGAA	2 cut(s)	397, 825
BspTI	CTTAAG	1 cut(s)	659
BsrDI	GCAATG	1 cut(s)	1405
BsrFI	RCCGGY	1 cut(s)	832
BsrI	ACTGG	3 cut(s)	138, 1089, 1256
BssAI	RCCGGY	1 cut(s)	832
BssECI	CCNNGG	5 cut(s)	360, 859, 1089, 1234, 1325
BssMI	GATC	3 cut(s)	346, 1059, 1171
BssT1I	CCWWGG	3 cut(s)	360, 1089, 1234
Bst2UI	CCWGG	2 cut(s)	453, 1327
Bst4CI	ACNGT	4 cut(s)	33, 175, 543, 632
Bst6I	CTCTTC	3 cut(s)	27, 822, 827
BstAFI	CTTAAG	1 cut(s)	659
BstAPI	GCANNNNNTGC	2 cut(s)	608, 1125
BstBAI	YACGTR	1 cut(s)	710
BstBI	TTCGAA	2 cut(s)	397, 825
BstC8I	GCNNGC	5 cut(s)	382, 1102, 1117, 1267, 1271
BstDEI	CTNAG	2 cut(s)	372, 1432
BstDSI	CCRYGG	3 cut(s)	360, 1089, 1234
BstF5I	GGATG	2 cut(s)	748, 1335
BstFNI	CGCG	1 cut(s)	247
BstHHI	GCGC	1 cut(s)	226
BstKTI	GATC	3 cut(s)	349, 1062, 1174
BstMAI	GTCTC	3 cut(s)	158, 532, 1070
BstMBI	GATC	3 cut(s)	346, 1059, 1171
BstMWI	GCNNNNNNNGC	9 cut(s)	223, 377, 605, 608, 617, 710, 1040, 1125, 1159
BstNI	CCWGG	2 cut(s)	453, 1327
BstSCI	CCNGG	3 cut(s)	276, 451, 1325
BstSFI	CTRYAG	2 cut(s)	339, 597
BstUI	CGCG	1 cut(s)	247
BstV1I	GCAGC	7 cut(s)	595, 611, 920, 1052, 1099, 1146, 1309
BstX2I	RGATCY	1 cut(s)	1171
BstYI	RGATCY	1 cut(s)	1171
BsuRI	GGCC	6 cut(s)	128, 202, 858, 1088, 1104, 1233
BtgI	CCRYGG	3 cut(s)	360, 1089, 1234
BtgZI	GCGATG	1 cut(s)	422
BtsCI	GGATG	2 cut(s)	748, 1335
BtsIMutI	CAGTG	1 cut(s)	1249
Cac8I	GCNNGC	5 cut(s)	382, 1102, 1117, 1267, 1271
CaiI	CAGNNNCTG	2 cut(s)	608, 1303
CfoI	GCGC	1 cut(s)	226
Cfr10I	RCCGGY	1 cut(s)	832
Cfr13I	GGNCC	2 cut(s)	274, 835
CpoI	CGGWCCG	1 cut(s)	274
Csp6I	GTAC	1 cut(s)	628
CspAI	ACCGGT	1 cut(s)	832
CspI	CGGWCCG	1 cut(s)	274
CviAII	CATG	6 cut(s)	361, 458, 797, 1090, 1120, 1235
CviQI	GTAC	1 cut(s)	628
DdeI	CTNAG	2 cut(s)	372, 1432
DpnI	GATC	3 cut(s)	348, 1061, 1173
DpnII	GATC	3 cut(s)	346, 1059, 1171
DraI	TTTAAA	3 cut(s)	646, 654, 1287
DrdI	GACNNNNNNGTC	1 cut(s)	692
DseDI	GACNNNNNNGTC	1 cut(s)	692
EaeI	YGGCCR	3 cut(s)	126, 1086, 1102
Eam1104I	CTCTTC	3 cut(s)	27, 822, 827
EarI	CTCTTC	3 cut(s)	27, 822, 827
EciI	GGCGGA	1 cut(s)	1325
Ecl136II	GAGCTC	1 cut(s)	6
Eco130I	CCWWGG	3 cut(s)	360, 1089, 1234
Eco147I	AGGCCT	1 cut(s)	858
Eco24I	GRGCYC	2 cut(s)	8, 1015
Eco47I	GGWCC	2 cut(s)	274, 835
Eco53kI	GAGCTC	1 cut(s)	6
Eco88I	CYCGRG	1 cut(s)	161
EcoICRI	GAGCTC	1 cut(s)	6
EcoRI	GAATTC	2 cut(s)	679, 1379
EcoRII	CCWGG	2 cut(s)	451, 1325
EcoT14I	CCWWGG	3 cut(s)	360, 1089, 1234
EcoT38I	GRGCYC	2 cut(s)	8, 1015
ErhI	CCWWGG	3 cut(s)	360, 1089, 1234
Esp3I	CGTCTC	1 cut(s)	158
FaeI	CATG	6 cut(s)	364, 461, 800, 1093, 1123, 1238
FalI	AAGNNNNNCTT	2 cut(s)	948, 980
FaqI	GGGAC	2 cut(s)	512, 1268
FatI	CATG	6 cut(s)	360, 457, 796, 1089, 1119, 1234
FauI	CCCGC	1 cut(s)	1036
Fnu4HI	GCNGC	9 cut(s)	215, 248, 600, 609, 909, 1041, 1113, 1160, 1298
FokI	GGATG	2 cut(s)	755, 1342
FriOI	GRGCYC	2 cut(s)	8, 1015
Fsp4HI	GCNGC	9 cut(s)	215, 248, 600, 609, 909, 1041, 1113, 1160, 1298
FspBI	CTAG	3 cut(s)	1080, 1145, 1227
GlaI	GCGC	1 cut(s)	225
GluI	GCNGC	9 cut(s)	215, 248, 600, 609, 909, 1041, 1113, 1160, 1298
GsuI	CTGGAG	2 cut(s)	272, 1202
HaeIII	GGCC	6 cut(s)	128, 202, 858, 1088, 1104, 1233
HapII	CCGG	4 cut(s)	277, 404, 833, 1175
HhaI	GCGC	1 cut(s)	226
Hin1II	CATG	6 cut(s)	364, 461, 800, 1093, 1123, 1238
Hin6I	GCGC	1 cut(s)	224
HinP1I	GCGC	1 cut(s)	224
HincII	GTYRAC	1 cut(s)	1141
HindII	GTYRAC	1 cut(s)	1141
HindIII	AAGCTT	2 cut(s)	378, 656
HinfI	GANTC	3 cut(s)	196, 445, 811
HpaII	CCGG	4 cut(s)	277, 404, 833, 1175
HphI	GGTGA	5 cut(s)	21, 100, 187, 323, 710
Hpy166II	GTNNAC	6 cut(s)	171, 178, 231, 718, 871, 1141
Hpy188III	TCNNGA	4 cut(s)	163, 232, 785, 1181
Hpy8I	GTNNAC	6 cut(s)	171, 178, 231, 718, 871, 1141
HpyAV	CCTTC	3 cut(s)	632, 707, 746
HpyCH4III	ACNGT	4 cut(s)	33, 175, 543, 632
HpyCH4IV	ACGT	6 cut(s)	167, 180, 210, 413, 709, 876
HpyCH4V	TGCA	8 cut(s)	184, 341, 506, 599, 908, 1249, 1273, 1410
HpyF10VI	GCNNNNNNNGC	9 cut(s)	223, 377, 605, 608, 617, 710, 1040, 1125, 1159
HpyF3I	CTNAG	2 cut(s)	372, 1432
HpySE526I	ACGT	6 cut(s)	167, 180, 210, 413, 709, 876
Hsp92II	CATG	6 cut(s)	364, 461, 800, 1093, 1123, 1238
HspAI	GCGC	1 cut(s)	224
Kzo9I	GATC	3 cut(s)	346, 1059, 1171
LmnI	GCTCC	5 cut(s)	51, 64, 551, 1326, 1333
Lsp1109I	GCAGC	7 cut(s)	595, 611, 920, 1052, 1099, 1146, 1309
LweI	GCATC	4 cut(s)	493, 733, 1309, 1425
MaeI	CTAG	3 cut(s)	1080, 1145, 1227
MaeII	ACGT	6 cut(s)	167, 180, 210, 413, 709, 876
MaeIII	GTNAC	3 cut(s)	290, 334, 1187
MalI	GATC	3 cut(s)	348, 1061, 1173
MboI	GATC	3 cut(s)	346, 1059, 1171
MboII	GAAGA	7 cut(s)	14, 440, 647, 809, 814, 968, 1181
MfeI	CAATTG	1 cut(s)	9
MflI	RGATCY	1 cut(s)	1171
MhlI	GDGCHC	2 cut(s)	8, 1015
MlsI	TGGCCA	3 cut(s)	128, 1088, 1104
MluNI	TGGCCA	3 cut(s)	128, 1088, 1104
MlyI	GAGTC	1 cut(s)	820
MmeI	TCCRAC	1 cut(s)	789
Mox20I	TGGCCA	3 cut(s)	128, 1088, 1104
MroXI	GAANNNNTTC	1 cut(s)	696
MscI	TGGCCA	3 cut(s)	128, 1088, 1104
MslI	CAYNNNNRTG	1 cut(s)	843
Msp20I	TGGCCA	3 cut(s)	128, 1088, 1104
MspA1I	CMGCKG	3 cut(s)	217, 911, 1043
MspCI	CTTAAG	1 cut(s)	659
MspI	CCGG	4 cut(s)	277, 404, 833, 1175
MspR9I	CCNGG	3 cut(s)	278, 453, 1327
MunI	CAATTG	1 cut(s)	9
Mva1269I	GAATGC	1 cut(s)	1398
MvaI	CCWGG	2 cut(s)	453, 1327
MvnI	CGCG	1 cut(s)	247
MwoI	GCNNNNNNNGC	9 cut(s)	223, 377, 605, 608, 617, 710, 1040, 1125, 1159
NciI	CCSGG	1 cut(s)	278
NcoI	CCATGG	3 cut(s)	360, 1089, 1234
NdeII	GATC	3 cut(s)	346, 1059, 1171
NlaIII	CATG	6 cut(s)	364, 461, 800, 1093, 1123, 1238
NmuCI	GTSAC	1 cut(s)	334
NspV	TTCGAA	2 cut(s)	397, 825
PaeR7I	CTCGAG	1 cut(s)	161
PceI	AGGCCT	1 cut(s)	858
PcsI	WCGNNNNNNNCGW	1 cut(s)	706
PctI	GAATGC	1 cut(s)	1398
PdmI	GAANNNNTTC	1 cut(s)	696
PfeI	GAWTC	2 cut(s)	196, 445
PfoI	TCCNGGA	1 cut(s)	451
PinAI	ACCGGT	1 cut(s)	832
PkrI	GCNGC	9 cut(s)	216, 249, 601, 610, 910, 1042, 1114, 1161, 1299
PleI	GAGTC	1 cut(s)	819
PpsI	GAGTC	1 cut(s)	819
Ppu21I	YACGTR	1 cut(s)	710
PsiI	TTATAA	1 cut(s)	1212
Psp124BI	GAGCTC	1 cut(s)	8
Psp6I	CCWGG	2 cut(s)	451, 1325
PspGI	CCWGG	2 cut(s)	451, 1325
PspPI	GGNCC	2 cut(s)	274, 835
PstI	CTGCAG	2 cut(s)	343, 601
PstNI	CAGNNNCTG	2 cut(s)	608, 1303
PsuI	RGATCY	1 cut(s)	1171
PvuII	CAGCTG	1 cut(s)	911
RsaI	GTAC	1 cut(s)	629
RsaNI	GTAC	1 cut(s)	628
RseI	CAYNNNNRTG	1 cut(s)	843
Rsr2I	CGGWCCG	1 cut(s)	274
RsrII	CGGWCCG	1 cut(s)	274
SacI	GAGCTC	1 cut(s)	8
SatI	GCNGC	9 cut(s)	215, 248, 600, 609, 909, 1041, 1113, 1160, 1298
Sau3AI	GATC	3 cut(s)	346, 1059, 1171
Sau96I	GGNCC	2 cut(s)	274, 835
SchI	GAGTC	1 cut(s)	820
ScrFI	CCNGG	3 cut(s)	278, 453, 1327
SduI	GDGCHC	2 cut(s)	8, 1015
SfaNI	GCATC	4 cut(s)	493, 733, 1309, 1425
SfcI	CTRYAG	2 cut(s)	339, 597
Sfr274I	CTCGAG	1 cut(s)	161
SfuI	TTCGAA	2 cut(s)	397, 825
SinI	GGWCC	2 cut(s)	274, 835
SlaI	CTCGAG	1 cut(s)	161
SmiMI	CAYNNNNRTG	1 cut(s)	843
SmlI	CTYRAG	2 cut(s)	161, 659
SmoI	CTYRAG	2 cut(s)	161, 659
SseBI	AGGCCT	1 cut(s)	858
SsiI	CCGC	6 cut(s)	215, 247, 272, 1043, 1205, 1310
SspI	AATATT	1 cut(s)	1261
SspMI	CTAG	3 cut(s)	1080, 1145, 1227
SstI	GAGCTC	1 cut(s)	8
StuI	AGGCCT	1 cut(s)	858
StyD4I	CCNGG	3 cut(s)	276, 451, 1325
StyI	CCWWGG	3 cut(s)	360, 1089, 1234
TaaI	ACNGT	4 cut(s)	33, 175, 543, 632
TaiI	ACGT	6 cut(s)	170, 183, 213, 416, 712, 879
TaqI	TCGA	8 cut(s)	73, 162, 397, 700, 825, 925, 1009, 1026
TauI	GCSGC	2 cut(s)	217, 250
TfiI	GAWTC	2 cut(s)	196, 445
TscAI	CASTG	1 cut(s)	1256
TseFI	GTSAC	1 cut(s)	334
TseI	GCWGC	7 cut(s)	599, 608, 908, 1040, 1112, 1159, 1297
Tsp45I	GTSAC	1 cut(s)	334
TspDTI	ATGAA	2 cut(s)	1372, 1383
TspRI	CASTG	1 cut(s)	1256
Vha464I	CTTAAG	1 cut(s)	659
VpaK11BI	GGWCC	2 cut(s)	274, 835
XapI	RAATTY	5 cut(s)	104, 673, 679, 1051, 1379
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1112
XhoI	CTCGAG	1 cut(s)	161
XmnI	GAANNNNTTC	1 cut(s)	696
XspI	CTAG	3 cut(s)	1080, 1145, 1227

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.