Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0005635

Physical Location & Seq

Genomic Coordinates Reverse (-)

35212309 .. 35214840

Gene Structure 2532 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1220000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1161 bp

ATGGCTCTGGTCACCCCCACCACTTTTGGGTTCAGCAATCTGCTGGAAACCTTCACGGTGAACGTGCAGAGAGCAGAAAACCGGCCGCTGAACGTGCCGCTGACTGCACCGTTCACGATTGCGACTTCGCGTCTGGAGAGGGTGGAGAATGTGGCCGTGCGGGTTGAGTTGAGTAACGGGTGCGTGGGGTGGGGTGAAACTCCGATCTTGCCCTTTGTGACTGCAGAGGATCAGCAGACAGCCATGGTTAAGGCTAAGGAGGCCTGCGAGTTCTTGCTTCGCAGTCCGGCGATGACGTTGGGTTCGGTTTTGGGGGAGATTGGTGGGATTCTTCCTGGATATGAATTTGCTTCTGTTAGGGCAGGAGTTGAGATGGCAATTATCGATGCAGTTTCTATGAGTATCGGTGTCCCTTTGTGGAGACTATTTGGTGGAGCTTCAAACACAGTAATTACTGATATAACAATTCCGATTGTTTCTGCAGCTGAAGCTGCTAAATTAGCTTCAAAGTACCGTGAACAAGGATTTAAGACCTTAAAGCTTAAGGTGGGAAAGAATTTGAATTCAGACATAGAAGTCCTTCAAGCTATACGTGATGTTCACCCTGATTGCTATTTTATCTTGGATGCTAACGAAGGATATAACTCTGATGAAGCTATCCAAGTTCTTGAAAAATTACATGAGGTGGGAGTGAGTCCTCTTCTCTTCGAACAACCGGTTCATAGAGATGATTGGAAAGGCCTTGGAAATGTTTACCGCGTTGCGAAAGACAAATATGGAGTATCAGTTGCAGCTGATGAGAGCTGTCGAAGTTTGGCTGATGTTAAGAAAATAGTGGAAGAAAATCTTGCTGATGTAATTAATATTAAACTTGCTAAAGTTGGGGTTGTTCGAGCCCTTGAAATCATCGAGGTAGCGAAGGCAGCGGGATTGGATCTGATGATTGGTGGTATGGTCGAGACTAGACTGGCCATGGGTTTTGCTGGCCATCTTGCTGCTGGCATGGGGTGCTTTAATTTCCTGAAATTCTTTCGCTTTCTATTTCCACATCCATCAGTAAGTGTTTGCATTCTGCACAGGTTTGTTGACCTAGACACGCCCCTGCTGCTATCAGAAGATCCGGTTCTGGAGGGTTACGAAGGTATTGCGATTTCTCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016853 GO:0016854 GO:0016855 GO:0034641 GO:0043167 GO:0043169 GO:0043603 GO:0044237 GO:0046872 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MR_MLE_C MR_MLE_N

Protein Analysis

387

Amino Acids

41.88

Weight (kDa)

5.11

Isoelectric Point (pI)

27.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MR_MLE_N	PF02746	32 - 144	2.8e-06	Mandelate racemase / muconate lactonizing enzyme, N-terminal domain
MR_MLE_C	PF13378	164 - 347	4.1e-34	Enolase C-terminal domain-like
MAAL_C	PF07476	199 - 330	1.7e-08	Methylaspartate ammonia-lyase C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005635)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18270
fragaria_vesca	FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670
malus_domestica	MD06G1220000.v1.1 MD14G1229600.v1.1
prunus_persica	Prupe.5G224400_v2.0.a1 Prupe.5G224400_v2.0.a1
pyrus_communis	pycom14g19100 pycom14g19110
rosa_chinensis	RchiOBHm_Chr7g0179451
rosa_laevigata	RLG00000005355
rosa_multiflora	Rmu_co8470985.1_g000001
rosa_roxburghii	Rroxscaffold_3G00274010
rosa_rugosa	Rorug06G0424300 Rorug06G0424400 Rorug06G0424500 Rorug06G0424600
rosa_samantha	Rh2AG318700 Rh6AG144500 Rh7BG023500
rosa_wichuraiana	Rw4G009460 Rw4G033460 Rw5G048300 Rw7G001940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	575
AccII	CGCG	2 cut(s)	130, 759
AciI	CCGC	5 cut(s)	86, 98, 160, 757, 926
AclWI	GGATC	3 cut(s)	237, 942, 1112
AcoI	YGGCCR	4 cut(s)	83, 153, 969, 985
AcsI	RAATTY	4 cut(s)	344, 556, 562, 1025
AcuI	CTGAAG	1 cut(s)	507
AfaI	GTAC	1 cut(s)	512
AfiI	CCNNNNNNNGG	1 cut(s)	27
AflII	CTTAAG	1 cut(s)	542
AgeI	ACCGGT	1 cut(s)	715
AgsI	TTSAA	6 cut(s)	441, 507, 562, 584, 671, 902
AhdI	GACNNNNNGTC	1 cut(s)	129
AjnI	CCWGG	1 cut(s)	334
AluBI	AGCT	9 cut(s)	437, 485, 491, 503, 541, 587, 656, 794, 804
AluI	AGCT	9 cut(s)	437, 485, 491, 503, 541, 587, 656, 794, 804
Alw26I	GTCTC	2 cut(s)	415, 953
AlwI	GGATC	3 cut(s)	237, 942, 1112
AoxI	GGCC	6 cut(s)	83, 153, 261, 739, 969, 985
ApeKI	GCWGC	6 cut(s)	482, 491, 791, 923, 995, 1105
ApoI	RAATTY	4 cut(s)	344, 556, 562, 1025
ArsI	GACNNNNNNTTYG	2 cut(s)	286, 318
AseI	ATTAAT	1 cut(s)	861
AsiGI	ACCGGT	1 cut(s)	715
Asp700I	GAANNNNTTC	1 cut(s)	579
AsuHPI	GGTGA	4 cut(s)	4, 70, 206, 593
AsuII	TTCGAA	1 cut(s)	708
BalI	TGGCCA	2 cut(s)	971, 987
BanII	GRGCYC	1 cut(s)	898
BbvI	GCAGC	6 cut(s)	478, 494, 803, 935, 982, 1092
BccI	CCATC	3 cut(s)	367, 996, 1060
BceAI	ACGGC	1 cut(s)	140
BcgI	CGANNNNNNTGC	2 cut(s)	1127, 1161
BciT130I	CCWGG	1 cut(s)	336
BcoDI	GTCTC	2 cut(s)	415, 953
BfaI	CTAG	2 cut(s)	963, 1091
BfmI	CTRYAG	2 cut(s)	222, 480
BfrI	CTTAAG	1 cut(s)	542
BisI	GCNGC	8 cut(s)	86, 98, 483, 492, 792, 924, 996, 1106
BlsI	GCNGC	8 cut(s)	87, 99, 484, 493, 793, 925, 997, 1107
Bme1390I	CCNGG	1 cut(s)	336
BmeRI	GACNNNNNGTC	1 cut(s)	129
BmrFI	CCNGG	1 cut(s)	336
BmsI	GCATC	2 cut(s)	376, 616
BpmI	CTGGAG	2 cut(s)	155, 1148
Bpu10I	CCTNAGC	1 cut(s)	255
Bpu14I	TTCGAA	1 cut(s)	708
Bsa29I	ATCGAT	1 cut(s)	384
BsaAI	YACGTR	1 cut(s)	593
BsaJI	CCNNGG	3 cut(s)	243, 742, 972
BsaWI	WCCGGW	2 cut(s)	715, 1120
BsaXI	ACNNNNNCTCC	2 cut(s)	771, 801
Bsc4I	CCNNNNNNNGG	1 cut(s)	27
Bse118I	RCCGGY	2 cut(s)	81, 715
Bse1I	ACTGG	1 cut(s)	972
BseBI	CCWGG	1 cut(s)	336
BseCI	ATCGAT	1 cut(s)	384
BseDI	CCNNGG	3 cut(s)	243, 742, 972
BseGI	GGATG	2 cut(s)	631, 1048
BseLI	CCNNNNNNNGG	1 cut(s)	27
BseNI	ACTGG	1 cut(s)	972
BseX3I	CGGCCG	1 cut(s)	83
BseXI	GCAGC	6 cut(s)	478, 494, 803, 935, 982, 1092
BsgI	GTGCAG	3 cut(s)	86, 90, 1058
Bsh1236I	CGCG	2 cut(s)	130, 759
Bsh1285I	CGRYCG	1 cut(s)	86
BshFI	GGCC	6 cut(s)	85, 155, 263, 741, 971, 987
BshTI	ACCGGT	1 cut(s)	715
BshVI	ATCGAT	1 cut(s)	384
BsiEI	CGRYCG	1 cut(s)	86
BsiSI	CCGG	4 cut(s)	82, 287, 716, 1121
BslFI	GGGAC	1 cut(s)	395
BslI	CCNNNNNNNGG	1 cut(s)	27
BsmAI	GTCTC	2 cut(s)	415, 953
BsmFI	GGGAC	1 cut(s)	395
BsmI	GAATGC	1 cut(s)	1068
BsnI	GGCC	6 cut(s)	85, 155, 263, 741, 971, 987
Bsp119I	TTCGAA	1 cut(s)	708
Bsp1286I	GDGCHC	1 cut(s)	898
Bsp143I	GATC	4 cut(s)	204, 229, 934, 1117
Bsp19I	CCATGG	2 cut(s)	243, 972
BspACI	CCGC	5 cut(s)	86, 98, 160, 757, 926
BspANI	GGCC	6 cut(s)	85, 155, 263, 741, 971, 987
BspDI	ATCGAT	1 cut(s)	384
BspFNI	CGCG	2 cut(s)	130, 759
BspMAI	CTGCAG	2 cut(s)	226, 484
BspPI	GGATC	3 cut(s)	237, 942, 1112
BspT104I	TTCGAA	1 cut(s)	708
BspTI	CTTAAG	1 cut(s)	542
BsrFI	RCCGGY	2 cut(s)	81, 715
BsrI	ACTGG	1 cut(s)	972
BssAI	RCCGGY	2 cut(s)	81, 715
BssECI	CCNNGG	3 cut(s)	243, 742, 972
BssMI	GATC	4 cut(s)	204, 229, 934, 1117
BssT1I	CCWWGG	3 cut(s)	243, 742, 972
Bst2UI	CCWGG	1 cut(s)	336
Bst4CI	ACNGT	4 cut(s)	58, 111, 448, 515
Bst6I	CTCTTC	2 cut(s)	705, 710
BstAFI	CTTAAG	1 cut(s)	542
BstAPI	GCANNNNNTGC	1 cut(s)	1008
BstBAI	YACGTR	1 cut(s)	593
BstBI	TTCGAA	1 cut(s)	708
BstC8I	GCNNGC	3 cut(s)	265, 985, 1000
BstDEI	CTNAG	1 cut(s)	255
BstDSI	CCRYGG	2 cut(s)	243, 972
BstEII	GGTNACC	1 cut(s)	10
BstF5I	GGATG	2 cut(s)	631, 1048
BstFNI	CGCG	2 cut(s)	130, 759
BstKTI	GATC	4 cut(s)	207, 232, 937, 1120
BstMAI	GTCTC	2 cut(s)	415, 953
BstMBI	GATC	4 cut(s)	204, 229, 934, 1117
BstMCI	CGRYCG	1 cut(s)	86
BstMWI	GCNNNNNNNGC	8 cut(s)	94, 260, 488, 491, 500, 923, 1008, 1105
BstNI	CCWGG	1 cut(s)	336
BstPI	GGTNACC	1 cut(s)	10
BstSCI	CCNGG	1 cut(s)	334
BstSFI	CTRYAG	2 cut(s)	222, 480
BstUI	CGCG	2 cut(s)	130, 759
BstV1I	GCAGC	6 cut(s)	478, 494, 803, 935, 982, 1092
BstX2I	RGATCY	2 cut(s)	934, 1117
BstYI	RGATCY	2 cut(s)	934, 1117
BstZI	CGGCCG	1 cut(s)	83
Bsu15I	ATCGAT	1 cut(s)	384
BsuRI	GGCC	6 cut(s)	85, 155, 263, 741, 971, 987
BsuTUI	ATCGAT	1 cut(s)	384
BtgI	CCRYGG	2 cut(s)	243, 972
BtgZI	GCGATG	1 cut(s)	305
BtsCI	GGATG	2 cut(s)	631, 1048
Cac8I	GCNNGC	3 cut(s)	265, 985, 1000
Cfr10I	RCCGGY	2 cut(s)	81, 715
ClaI	ATCGAT	1 cut(s)	384
CseI	GACGC	1 cut(s)	119
Csp6I	GTAC	1 cut(s)	511
CspAI	ACCGGT	1 cut(s)	715
CspCI	CAANNNNNGTGG	2 cut(s)	7, 42
CviAII	CATG	4 cut(s)	244, 680, 973, 1003
CviQI	GTAC	1 cut(s)	511
DdeI	CTNAG	1 cut(s)	255
DpnI	GATC	4 cut(s)	206, 231, 936, 1119
DpnII	GATC	4 cut(s)	204, 229, 934, 1117
DrdI	GACNNNNNNGTC	1 cut(s)	575
DriI	GACNNNNNGTC	1 cut(s)	129
DseDI	GACNNNNNNGTC	1 cut(s)	575
EaeI	YGGCCR	4 cut(s)	83, 153, 969, 985
EagI	CGGCCG	1 cut(s)	83
Eam1104I	CTCTTC	2 cut(s)	705, 710
Eam1105I	GACNNNNNGTC	1 cut(s)	129
EarI	CTCTTC	2 cut(s)	705, 710
EclXI	CGGCCG	1 cut(s)	83
Eco130I	CCWWGG	3 cut(s)	243, 742, 972
Eco147I	AGGCCT	2 cut(s)	263, 741
Eco24I	GRGCYC	1 cut(s)	898
Eco52I	CGGCCG	1 cut(s)	83
Eco57I	CTGAAG	1 cut(s)	507
Eco91I	GGTNACC	1 cut(s)	10
EcoO65I	GGTNACC	1 cut(s)	10
EcoRI	GAATTC	1 cut(s)	562
EcoRII	CCWGG	1 cut(s)	334
EcoT14I	CCWWGG	3 cut(s)	243, 742, 972
EcoT38I	GRGCYC	1 cut(s)	898
ErhI	CCWWGG	3 cut(s)	243, 742, 972
FaeI	CATG	4 cut(s)	247, 683, 976, 1006
FalI	AAGNNNNNCTT	2 cut(s)	831, 863
FaqI	GGGAC	1 cut(s)	395
FatI	CATG	4 cut(s)	243, 679, 972, 1002
FauI	CCCGC	2 cut(s)	153, 919
Fnu4HI	GCNGC	8 cut(s)	86, 98, 483, 492, 792, 924, 996, 1106
FokI	GGATG	2 cut(s)	638, 1035
FriOI	GRGCYC	1 cut(s)	898
Fsp4HI	GCNGC	8 cut(s)	86, 98, 483, 492, 792, 924, 996, 1106
FspBI	CTAG	2 cut(s)	963, 1091
GluI	GCNGC	8 cut(s)	86, 98, 483, 492, 792, 924, 996, 1106
GsuI	CTGGAG	2 cut(s)	155, 1148
HaeIII	GGCC	6 cut(s)	85, 155, 263, 741, 971, 987
HapII	CCGG	4 cut(s)	82, 287, 716, 1121
HgaI	GACGC	1 cut(s)	119
Hin1II	CATG	4 cut(s)	247, 683, 976, 1006
HincII	GTYRAC	1 cut(s)	1087
HindII	GTYRAC	1 cut(s)	1087
HindIII	AAGCTT	1 cut(s)	539
HinfI	GANTC	2 cut(s)	328, 694
HpaII	CCGG	4 cut(s)	82, 287, 716, 1121
HphI	GGTGA	4 cut(s)	4, 70, 206, 593
Hpy166II	GTNNAC	6 cut(s)	61, 114, 518, 601, 754, 1087
Hpy188I	TCNGA	6 cut(s)	204, 471, 568, 649, 939, 1114
Hpy188III	TCNNGA	6 cut(s)	115, 134, 668, 958, 1021, 1127
Hpy8I	GTNNAC	6 cut(s)	61, 114, 518, 601, 754, 1087
HpyAV	CCTTC	5 cut(s)	61, 590, 629, 913, 1133
HpyCH4III	ACNGT	4 cut(s)	58, 111, 448, 515
HpyCH4IV	ACGT	4 cut(s)	63, 93, 296, 592
HpyCH4V	TGCA	8 cut(s)	67, 107, 224, 389, 482, 791, 1068, 1075
HpyF10VI	GCNNNNNNNGC	8 cut(s)	94, 260, 488, 491, 500, 923, 1008, 1105
HpyF3I	CTNAG	1 cut(s)	255
HpySE526I	ACGT	4 cut(s)	63, 93, 296, 592
Hsp92II	CATG	4 cut(s)	247, 683, 976, 1006
Kzo9I	GATC	4 cut(s)	204, 229, 934, 1117
LmnI	GCTCC	1 cut(s)	434
Lsp1109I	GCAGC	6 cut(s)	478, 494, 803, 935, 982, 1092
LweI	GCATC	2 cut(s)	376, 616
MaeI	CTAG	2 cut(s)	963, 1091
MaeII	ACGT	4 cut(s)	63, 93, 296, 592
MaeIII	GTNAC	4 cut(s)	10, 173, 217, 1133
MalI	GATC	4 cut(s)	206, 231, 936, 1119
MboI	GATC	4 cut(s)	204, 229, 934, 1117
MboII	GAAGA	5 cut(s)	323, 692, 697, 851, 1127
MflI	RGATCY	2 cut(s)	934, 1117
MhlI	GDGCHC	1 cut(s)	898
MlsI	TGGCCA	2 cut(s)	971, 987
MluNI	TGGCCA	2 cut(s)	971, 987
MlyI	GAGTC	1 cut(s)	703
MnlI	CCTC	7 cut(s)	132, 220, 253, 676, 708, 904, 1123
Mox20I	TGGCCA	2 cut(s)	971, 987
MroXI	GAANNNNTTC	1 cut(s)	579
MscI	TGGCCA	2 cut(s)	971, 987
MseI	TTAA	8 cut(s)	249, 528, 536, 543, 825, 861, 867, 1014
MslI	CAYNNNNRTG	1 cut(s)	726
Msp20I	TGGCCA	2 cut(s)	971, 987
MspA1I	CMGCKG	5 cut(s)	88, 100, 485, 794, 926
MspCI	CTTAAG	1 cut(s)	542
MspI	CCGG	4 cut(s)	82, 287, 716, 1121
MspR9I	CCNGG	1 cut(s)	336
Mva1269I	GAATGC	1 cut(s)	1068
MvaI	CCWGG	1 cut(s)	336
MvnI	CGCG	2 cut(s)	130, 759
MwoI	GCNNNNNNNGC	8 cut(s)	94, 260, 488, 491, 500, 923, 1008, 1105
NcoI	CCATGG	2 cut(s)	243, 972
NdeII	GATC	4 cut(s)	204, 229, 934, 1117
NlaIII	CATG	4 cut(s)	247, 683, 976, 1006
NmuCI	GTSAC	2 cut(s)	10, 217
NspV	TTCGAA	1 cut(s)	708
PceI	AGGCCT	2 cut(s)	263, 741
PctI	GAATGC	1 cut(s)	1068
PdmI	GAANNNNTTC	1 cut(s)	579
PfeI	GAWTC	1 cut(s)	328
PfoI	TCCNGGA	1 cut(s)	334
PinAI	ACCGGT	1 cut(s)	715
PkrI	GCNGC	8 cut(s)	87, 99, 484, 493, 793, 925, 997, 1107
PleI	GAGTC	1 cut(s)	702
PpsI	GAGTC	1 cut(s)	702
Ppu21I	YACGTR	1 cut(s)	593
PshBI	ATTAAT	1 cut(s)	861
Psp6I	CCWGG	1 cut(s)	334
PspEI	GGTNACC	1 cut(s)	10
PspGI	CCWGG	1 cut(s)	334
PstI	CTGCAG	2 cut(s)	226, 484
PsuI	RGATCY	2 cut(s)	934, 1117
PvuII	CAGCTG	2 cut(s)	485, 794
RsaI	GTAC	1 cut(s)	512
RsaNI	GTAC	1 cut(s)	511
RseI	CAYNNNNRTG	1 cut(s)	726
SaqAI	TTAA	8 cut(s)	249, 528, 536, 543, 825, 861, 867, 1014
SatI	GCNGC	8 cut(s)	86, 98, 483, 492, 792, 924, 996, 1106
Sau3AI	GATC	4 cut(s)	204, 229, 934, 1117
SchI	GAGTC	1 cut(s)	703
ScrFI	CCNGG	1 cut(s)	336
SduI	GDGCHC	1 cut(s)	898
SfaNI	GCATC	2 cut(s)	376, 616
SfcI	CTRYAG	2 cut(s)	222, 480
SfuI	TTCGAA	1 cut(s)	708
SmiMI	CAYNNNNRTG	1 cut(s)	726
SmlI	CTYRAG	1 cut(s)	542
SmoI	CTYRAG	1 cut(s)	542
SseBI	AGGCCT	2 cut(s)	263, 741
SsiI	CCGC	5 cut(s)	86, 98, 160, 757, 926
SspI	AATATT	1 cut(s)	865
SspMI	CTAG	2 cut(s)	963, 1091
StuI	AGGCCT	2 cut(s)	263, 741
StyD4I	CCNGG	1 cut(s)	334
StyI	CCWWGG	3 cut(s)	243, 742, 972
TaaI	ACNGT	4 cut(s)	58, 111, 448, 515
TaiI	ACGT	4 cut(s)	66, 96, 299, 595
TaqI	TCGA	6 cut(s)	384, 708, 808, 892, 909, 957
TauI	GCSGC	2 cut(s)	88, 100
TfiI	GAWTC	1 cut(s)	328
Tru1I	TTAA	8 cut(s)	249, 528, 536, 543, 825, 861, 867, 1014
Tru9I	TTAA	8 cut(s)	249, 528, 536, 543, 825, 861, 867, 1014
TseFI	GTSAC	2 cut(s)	10, 217
TseI	GCWGC	6 cut(s)	482, 491, 791, 923, 995, 1105
Tsp45I	GTSAC	2 cut(s)	10, 217
TspDTI	ATGAA	3 cut(s)	357, 666, 710
Vha464I	CTTAAG	1 cut(s)	542
VspI	ATTAAT	1 cut(s)	861
XapI	RAATTY	4 cut(s)	344, 556, 562, 1025
XcmI	CCANNNNNNNNNTGG	1 cut(s)	995
XmnI	GAANNNNTTC	1 cut(s)	579
XspI	CTAG	2 cut(s)	963, 1091

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.