Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0005635

Physical Location & Seq

Genomic Coordinates Reverse (-)

65746579 .. 65750994

Gene Structure 4416 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00274010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1152 bp

ATGGCTCTGGCCACCCCCACCAGATTTGGGTTCAGTAATTTACTCGAGACTTTCACAGTCAACGTTCAGAGAGCAGAGAACAGGCCTCTGAATGTGCCTCTGATTGCTCCTTTTACGATTGCGACTTCGAGGCTTGAACGTGTTGAGAATGTTGCGATCAGAGTTGAGTTGAGAAATGGGTGTGTGGGGTGGGGTGAAGCTCCAATCTTGCCTCATGTGACTGCAGAGGATCAGCACACCGCTATGGTCAAGGCCAAGGAGGCTTGTGAGTTCTTGCTCAGGAATAATGGGATGACTTTGAGTTCGGTTTTGGGAGAGATTGGTGTGCTTCTCCCTGGATATGAGTTTGCTTCTGTTAGGGCAGGAGTCGAGATGGCTCTAATTGACGCAGTTGCTACAAGTATTGGTGTCCCTTTGTGGAGACTGTTTGGTGGAGCTTCAAATACTATAACCACTGATATAACGATTCCGATAGTTTCTGCAGCTGAAGCTGCCAGACTGGCTTCAAAGTACTGCAAAGAAGGATTTAGAACTTTAAAGCTTAAGGTGGGAAAGAATCTAAATTCAGACATAGAAGTCCTTCAAGCTATACGGGCAGTCCACCCTGATTGCTCTTTTATCTTGGATGCTAACGAAGGATATAACTCCAATGAAGCTATCCAAGTTCTTGAGAAATTATATGAGGTGGGAGTGAGTCCAGTTCTCTTTGAACAACCAGTTCATAGAGATGATTGGGAAGGCCTTGGATATGTTACTCGTATTGCTAGAGAAAGATACGGAGTATCAGTTGCAGCTGATGAGAGCTGTCGAAGTATAGTAGATGTTAAGAAAATAGCAGAAGAAAATCTTGCTAATGTCATTAACATTAAACTTACTAAAGTTGGGGTTGTTGGGGCCCTTGAAATCATTGGGGTTGCAAGAGCATCGGGATTGGATTTGATGATTGGTGGTATGGTTGAGACTAGACTCGCCATGGGCTTTTCTGGCCACCTTGCTGCTGGCCTTGGGTGTTTTAAGTTTATTGATCTAGATACGCCCCTATTGCTGTCAGAAGATCCAGTTCTGGAGGGTTATGAAGTTTCGGGTGCCGTTTATAAGTTCAACAACGCTAGAGGCCATGGTGGATTTCTCCACTGGGACAACCTTGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000287 GO:0003674 GO:0003824 GO:0005488 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016853 GO:0016854 GO:0016855 GO:0034641 GO:0043167 GO:0043169 GO:0043603 GO:0044237 GO:0046872 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MR_MLE_C MR_MLE_N

Protein Analysis

383

Amino Acids

41.41

Weight (kDa)

5.33

Isoelectric Point (pI)

22.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MR_MLE_N	PF02746	33 - 144	3.7e-07	Mandelate racemase / muconate lactonizing enzyme, N-terminal domain
MR_MLE_C	PF13378	164 - 374	1e-33	Enolase C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005635)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18270
fragaria_vesca	FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670 FvH4_5g16670
malus_domestica	MD06G1220000.v1.1 MD14G1229600.v1.1
prunus_persica	Prupe.5G224400_v2.0.a1 Prupe.5G224400_v2.0.a1
pyrus_communis	pycom14g19100 pycom14g19110
rosa_chinensis	RchiOBHm_Chr7g0179451
rosa_laevigata	RLG00000005355
rosa_multiflora	Rmu_co8470985.1_g000001
rosa_roxburghii	Rroxscaffold_3G00274010
rosa_rugosa	Rorug06G0424300 Rorug06G0424400 Rorug06G0424500 Rorug06G0424600
rosa_samantha	Rh2AG318700 Rh6AG144500 Rh7BG023500
rosa_wichuraiana	Rw4G009460 Rw4G033460 Rw5G048300 Rw7G001940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1095
AasI	GACNNNNNNGTC	1 cut(s)	575
AccB1I	GGYRCC	1 cut(s)	1085
AciI	CCGC	1 cut(s)	240
AclI	AACGTT	1 cut(s)	63
AclWI	GGATC	2 cut(s)	237, 1049
AcoI	YGGCCR	2 cut(s)	9, 985
AcsI	RAATTY	1 cut(s)	562
AcuI	CTGAAG	1 cut(s)	507
AfaI	GTAC	1 cut(s)	512
AfiI	CCNNNNNNNGG	1 cut(s)	27
AflII	CTTAAG	1 cut(s)	542
AflIII	ACRYGT	1 cut(s)	139
AgsI	TTSAA	7 cut(s)	137, 441, 507, 584, 710, 902, 1102
AjnI	CCWGG	1 cut(s)	334
AluBI	AGCT	9 cut(s)	200, 437, 485, 491, 541, 587, 656, 794, 804
AluI	AGCT	9 cut(s)	200, 437, 485, 491, 541, 587, 656, 794, 804
Alw26I	GTCTC	3 cut(s)	41, 415, 953
AlwI	GGATC	2 cut(s)	237, 1049
Ama87I	CYCGRG	1 cut(s)	44
AoxI	GGCC	8 cut(s)	9, 83, 252, 739, 894, 985, 1000, 1114
ApaI	GGGCCC	1 cut(s)	898
ApeKI	GCWGC	4 cut(s)	482, 491, 791, 995
ApoI	RAATTY	1 cut(s)	562
ArsI	GACNNNNNNTTYG	2 cut(s)	286, 318
Asp700I	GAANNNNTTC	1 cut(s)	579
AspS9I	GGNCC	2 cut(s)	894, 895
AsuHPI	GGTGA	1 cut(s)	206
AvaI	CYCGRG	1 cut(s)	44
BaeGI	GKGCMC	1 cut(s)	898
BalI	TGGCCA	2 cut(s)	11, 987
BanI	GGYRCC	1 cut(s)	1085
BanII	GRGCYC	1 cut(s)	898
BbvI	GCAGC	4 cut(s)	478, 494, 803, 982
BccI	CCATC	1 cut(s)	367
BceAI	ACGGC	1 cut(s)	1073
BcgI	CGANNNNNNTGC	2 cut(s)	906, 940
BciT130I	CCWGG	1 cut(s)	336
BcoDI	GTCTC	3 cut(s)	41, 415, 953
BfaI	CTAG	4 cut(s)	765, 963, 1028, 1110
BfmI	CTRYAG	2 cut(s)	222, 480
BfrI	CTTAAG	1 cut(s)	542
BglI	GCCNNNNNGGC	2 cut(s)	260, 500
BisI	GCNGC	4 cut(s)	483, 492, 792, 996
BlsI	GCNGC	4 cut(s)	484, 493, 793, 997
BmcAI	AGTACT	1 cut(s)	512
Bme1390I	CCNGG	1 cut(s)	336
BmeT110I	CYCGRG	1 cut(s)	44
BmgT120I	GGNCC	2 cut(s)	894, 895
BmiI	GGNNCC	3 cut(s)	895, 896, 1087
BmrFI	CCNGG	1 cut(s)	336
BmrI	ACTGGG	1 cut(s)	1144
BmsI	GCATC	2 cut(s)	616, 932
BmuI	ACTGGG	1 cut(s)	1144
BpmI	CTGGAG	1 cut(s)	1085
Bpu10I	CCTNAGC	1 cut(s)	278
BpuEI	CTTGAG	1 cut(s)	689
BsaJI	CCNNGG	6 cut(s)	255, 334, 742, 972, 1003, 1117
BsaXI	ACNNNNNCTCC	2 cut(s)	771, 801
Bsc4I	CCNNNNNNNGG	1 cut(s)	27
Bse1I	ACTGG	5 cut(s)	504, 698, 716, 1058, 1139
BseBI	CCWGG	1 cut(s)	336
BseDI	CCNNGG	6 cut(s)	255, 334, 742, 972, 1003, 1117
BseGI	GGATG	2 cut(s)	297, 631
BseLI	CCNNNNNNNGG	1 cut(s)	27
BseMII	CTCAG	1 cut(s)	292
BseNI	ACTGG	5 cut(s)	504, 698, 716, 1058, 1139
BseSI	GKGCMC	1 cut(s)	898
BseXI	GCAGC	4 cut(s)	478, 494, 803, 982
BshFI	GGCC	8 cut(s)	11, 85, 254, 741, 896, 987, 1002, 1116
BshNI	GGYRCC	1 cut(s)	1085
BsiHKCI	CYCGRG	1 cut(s)	44
BslFI	GGGAC	1 cut(s)	395
BslI	CCNNNNNNNGG	1 cut(s)	27
BsmAI	GTCTC	3 cut(s)	41, 415, 953
BsmFI	GGGAC	1 cut(s)	395
BsnI	GGCC	8 cut(s)	11, 85, 254, 741, 896, 987, 1002, 1116
BsoBI	CYCGRG	1 cut(s)	44
Bsp120I	GGGCCC	1 cut(s)	894
Bsp1286I	GDGCHC	1 cut(s)	898
Bsp143I	GATC	4 cut(s)	156, 229, 1024, 1054
Bsp19I	CCATGG	2 cut(s)	972, 1117
BspACI	CCGC	1 cut(s)	240
BspANI	GGCC	8 cut(s)	11, 85, 254, 741, 896, 987, 1002, 1116
BspCNI	CTCAG	1 cut(s)	291
BspLI	GGNNCC	3 cut(s)	895, 896, 1087
BspMAI	CTGCAG	2 cut(s)	226, 484
BspPI	GGATC	2 cut(s)	237, 1049
BspT107I	GGYRCC	1 cut(s)	1085
BspTI	CTTAAG	1 cut(s)	542
BsrI	ACTGG	5 cut(s)	504, 698, 716, 1058, 1139
BssECI	CCNNGG	6 cut(s)	255, 334, 742, 972, 1003, 1117
BssMI	GATC	4 cut(s)	156, 229, 1024, 1054
BssT1I	CCWWGG	5 cut(s)	255, 742, 972, 1003, 1117
Bst2UI	CCWGG	1 cut(s)	336
Bst4CI	ACNGT	2 cut(s)	58, 426
BstAFI	CTTAAG	1 cut(s)	542
BstC8I	GCNNGC	1 cut(s)	1000
BstDEI	CTNAG	1 cut(s)	278
BstDSI	CCRYGG	2 cut(s)	972, 1117
BstF5I	GGATG	2 cut(s)	297, 631
BstKTI	GATC	4 cut(s)	159, 232, 1027, 1057
BstMAI	GTCTC	3 cut(s)	41, 415, 953
BstMBI	GATC	4 cut(s)	156, 229, 1024, 1054
BstMWI	GCNNNNNNNGC	7 cut(s)	260, 488, 491, 500, 593, 984, 1042
BstNI	CCWGG	1 cut(s)	336
BstSCI	CCNGG	1 cut(s)	334
BstSFI	CTRYAG	2 cut(s)	222, 480
BstSLI	GKGCMC	1 cut(s)	898
BstV1I	GCAGC	4 cut(s)	478, 494, 803, 982
BstX2I	RGATCY	1 cut(s)	1054
BstYI	RGATCY	1 cut(s)	1054
BsuRI	GGCC	8 cut(s)	11, 85, 254, 741, 896, 987, 1002, 1116
BtgI	CCRYGG	2 cut(s)	972, 1117
BtsCI	GGATG	2 cut(s)	297, 631
BtsIMutI	CAGTG	2 cut(s)	453, 1132
Cac8I	GCNNGC	1 cut(s)	1000
Cfr13I	GGNCC	2 cut(s)	894, 895
CseI	GACGC	1 cut(s)	395
Csp6I	GTAC	1 cut(s)	511
CspCI	CAANNNNNGTGG	4 cut(s)	7, 42, 590, 625
CviAII	CATG	3 cut(s)	215, 973, 1118
CviQI	GTAC	1 cut(s)	511
DdeI	CTNAG	1 cut(s)	278
DpnI	GATC	4 cut(s)	158, 231, 1026, 1056
DpnII	GATC	4 cut(s)	156, 229, 1024, 1054
DraI	TTTAAA	1 cut(s)	537
DrdI	GACNNNNNNGTC	1 cut(s)	575
DseDI	GACNNNNNNGTC	1 cut(s)	575
EaeI	YGGCCR	2 cut(s)	9, 985
Eco130I	CCWWGG	5 cut(s)	255, 742, 972, 1003, 1117
Eco147I	AGGCCT	2 cut(s)	85, 741
Eco24I	GRGCYC	1 cut(s)	898
Eco57I	CTGAAG	1 cut(s)	507
Eco88I	CYCGRG	1 cut(s)	44
EcoO109I	RGGNCCY	2 cut(s)	894, 895
EcoRII	CCWGG	1 cut(s)	334
EcoT14I	CCWWGG	5 cut(s)	255, 742, 972, 1003, 1117
EcoT38I	GRGCYC	1 cut(s)	898
ErhI	CCWWGG	5 cut(s)	255, 742, 972, 1003, 1117
FaeI	CATG	3 cut(s)	218, 976, 1121
FalI	AAGNNNNNCTT	2 cut(s)	831, 863
FaqI	GGGAC	1 cut(s)	395
FatI	CATG	3 cut(s)	214, 972, 1117
Fnu4HI	GCNGC	4 cut(s)	483, 492, 792, 996
FokI	GGATG	2 cut(s)	304, 638
FriOI	GRGCYC	1 cut(s)	898
Fsp4HI	GCNGC	4 cut(s)	483, 492, 792, 996
FspBI	CTAG	4 cut(s)	765, 963, 1028, 1110
GluI	GCNGC	4 cut(s)	483, 492, 792, 996
GsuI	CTGGAG	1 cut(s)	1085
HaeIII	GGCC	8 cut(s)	11, 85, 254, 741, 896, 987, 1002, 1116
HgaI	GACGC	1 cut(s)	395
Hin1II	CATG	3 cut(s)	218, 976, 1121
HincII	GTYRAC	1 cut(s)	61
HindII	GTYRAC	1 cut(s)	61
HindIII	AAGCTT	1 cut(s)	539
HinfI	GANTC	5 cut(s)	366, 466, 556, 694, 966
HphI	GGTGA	1 cut(s)	206
Hpy166II	GTNNAC	2 cut(s)	61, 601
Hpy188I	TCNGA	7 cut(s)	69, 90, 102, 161, 471, 568, 1051
Hpy188III	TCNNGA	7 cut(s)	46, 280, 370, 668, 927, 1028, 1064
Hpy8I	GTNNAC	2 cut(s)	61, 601
HpyAV	CCTTC	4 cut(s)	515, 590, 629, 731
HpyCH4III	ACNGT	2 cut(s)	58, 426
HpyCH4IV	ACGT	2 cut(s)	63, 139
HpyCH4V	TGCA	5 cut(s)	224, 482, 516, 791, 917
HpyF10VI	GCNNNNNNNGC	7 cut(s)	260, 488, 491, 500, 593, 984, 1042
HpyF3I	CTNAG	1 cut(s)	278
HpySE526I	ACGT	2 cut(s)	63, 139
Hsp92II	CATG	3 cut(s)	218, 976, 1121
Kzo9I	GATC	4 cut(s)	156, 229, 1024, 1054
LmnI	GCTCC	3 cut(s)	112, 205, 434
Lsp1109I	GCAGC	4 cut(s)	478, 494, 803, 982
LweI	GCATC	2 cut(s)	616, 932
MaeI	CTAG	4 cut(s)	765, 963, 1028, 1110
MaeII	ACGT	2 cut(s)	63, 139
MaeIII	GTNAC	2 cut(s)	217, 751
MalI	GATC	4 cut(s)	158, 231, 1026, 1056
MboI	GATC	4 cut(s)	156, 229, 1024, 1054
MboII	GAAGA	2 cut(s)	851, 1064
MflI	RGATCY	1 cut(s)	1054
MhlI	GDGCHC	1 cut(s)	898
MlsI	TGGCCA	2 cut(s)	11, 987
MluCI	AATT	4 cut(s)	37, 381, 562, 674
MluNI	TGGCCA	2 cut(s)	11, 987
MlyI	GAGTC	3 cut(s)	375, 703, 960
MnlI	CCTC	9 cut(s)	96, 108, 123, 220, 222, 253, 676, 1060, 1106
Mox20I	TGGCCA	2 cut(s)	11, 987
MroXI	GAANNNNTTC	1 cut(s)	579
MscI	TGGCCA	2 cut(s)	11, 987
MseI	TTAA	6 cut(s)	536, 543, 825, 861, 867, 1014
MslI	CAYNNNNRTG	2 cut(s)	242, 726
Msp20I	TGGCCA	2 cut(s)	11, 987
MspA1I	CMGCKG	2 cut(s)	485, 794
MspCI	CTTAAG	1 cut(s)	542
MspR9I	CCNGG	1 cut(s)	336
MvaI	CCWGG	1 cut(s)	336
MwoI	GCNNNNNNNGC	7 cut(s)	260, 488, 491, 500, 593, 984, 1042
NcoI	CCATGG	2 cut(s)	972, 1117
NdeII	GATC	4 cut(s)	156, 229, 1024, 1054
NlaIII	CATG	3 cut(s)	218, 976, 1121
NlaIV	GGNNCC	3 cut(s)	895, 896, 1087
NmuCI	GTSAC	1 cut(s)	217
PaeR7I	CTCGAG	1 cut(s)	44
PceI	AGGCCT	2 cut(s)	85, 741
PdmI	GAANNNNTTC	1 cut(s)	579
PfeI	GAWTC	2 cut(s)	466, 556
PkrI	GCNGC	4 cut(s)	484, 493, 793, 997
PleI	GAGTC	3 cut(s)	374, 702, 960
PpsI	GAGTC	3 cut(s)	374, 702, 960
PsiI	TTATAA	1 cut(s)	1095
Psp1406I	AACGTT	1 cut(s)	63
Psp6I	CCWGG	1 cut(s)	334
PspGI	CCWGG	1 cut(s)	334
PspN4I	GGNNCC	3 cut(s)	895, 896, 1087
PspOMI	GGGCCC	1 cut(s)	894
PspPI	GGNCC	2 cut(s)	894, 895
PstI	CTGCAG	2 cut(s)	226, 484
PsuI	RGATCY	1 cut(s)	1054
PvuII	CAGCTG	2 cut(s)	485, 794
RsaI	GTAC	1 cut(s)	512
RsaNI	GTAC	1 cut(s)	511
RseI	CAYNNNNRTG	2 cut(s)	242, 726
SaqAI	TTAA	6 cut(s)	536, 543, 825, 861, 867, 1014
SatI	GCNGC	4 cut(s)	483, 492, 792, 996
Sau3AI	GATC	4 cut(s)	156, 229, 1024, 1054
Sau96I	GGNCC	2 cut(s)	894, 895
ScaI	AGTACT	1 cut(s)	512
SchI	GAGTC	3 cut(s)	375, 703, 960
ScrFI	CCNGG	1 cut(s)	336
SduI	GDGCHC	1 cut(s)	898
SfaNI	GCATC	2 cut(s)	616, 932
SfcI	CTRYAG	2 cut(s)	222, 480
Sfr274I	CTCGAG	1 cut(s)	44
SlaI	CTCGAG	1 cut(s)	44
SmiMI	CAYNNNNRTG	2 cut(s)	242, 726
SmlI	CTYRAG	3 cut(s)	44, 542, 668
SmoI	CTYRAG	3 cut(s)	44, 542, 668
Sse9I	AATT	4 cut(s)	37, 381, 562, 674
SseBI	AGGCCT	2 cut(s)	85, 741
SsiI	CCGC	1 cut(s)	240
SspMI	CTAG	4 cut(s)	765, 963, 1028, 1110
StuI	AGGCCT	2 cut(s)	85, 741
StyD4I	CCNGG	1 cut(s)	334
StyI	CCWWGG	5 cut(s)	255, 742, 972, 1003, 1117
TaaI	ACNGT	2 cut(s)	58, 426
TaiI	ACGT	2 cut(s)	66, 142
TaqI	TCGA	4 cut(s)	45, 128, 369, 808
TasI	AATT	4 cut(s)	37, 381, 562, 674
TatI	WGTACW	1 cut(s)	510
TfiI	GAWTC	2 cut(s)	466, 556
Tru1I	TTAA	6 cut(s)	536, 543, 825, 861, 867, 1014
Tru9I	TTAA	6 cut(s)	536, 543, 825, 861, 867, 1014
TscAI	CASTG	2 cut(s)	460, 1139
TseFI	GTSAC	1 cut(s)	217
TseI	GCWGC	4 cut(s)	482, 491, 791, 995
Tsp45I	GTSAC	1 cut(s)	217
TspDTI	ATGAA	3 cut(s)	666, 710, 1089
TspGWI	ACGGA	1 cut(s)	792
TspRI	CASTG	2 cut(s)	460, 1139
Vha464I	CTTAAG	1 cut(s)	542
XapI	RAATTY	1 cut(s)	562
XbaI	TCTAGA	1 cut(s)	1027
XcmI	CCANNNNNNNNNTGG	1 cut(s)	995
XhoI	CTCGAG	1 cut(s)	44
XmnI	GAANNNNTTC	1 cut(s)	579
XspI	CTAG	4 cut(s)	765, 963, 1028, 1110
ZrmI	AGTACT	1 cut(s)	512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.