Rw4G033460

L-Ala-D L-amino acid

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005635
Physical Location & Seq
Reverse (-)
60635775 .. 60638005
2231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033460.1

Sequence Viewer

Length: 801 bp
ATGGCCTCCTCCCCCGGCCATTATCGGACTCGGAGCCTCCAACTCCACTCCCCGGGTCGGGTTCTTCCGCCACTCCAACCCCAAACTCGGCGAGCAGCTCCTCTACTTCATCCTCTCTTCCCTCTGTGGCCCAATTCAATCCGCCAAGGTTGTGCAAGGGTGGGAAAGAATCTAAATTCAGACATAGAAGTCCTTCAAGCTATAAGAGCAGTCCACTCTGATTGCTCTTTTATCTTGGACGCTAACGAACGATATAGCTCCAATGAAGCTATCCAAGTTCTTGAGAAATTATATGAGGTGGGAGTGAGTCCAGTTCTCTTTGAACAACCAGTTCATAGAGATGATTGGGAAGGCCTTGGATATGTTACTTGTATTGCTAGAGAAAGATATGGAGTATCAGTTGCAGCTGATGAGAGCTCTCGAAGTATAGTGGATGTTAAGAAAATAGCAGAAGAAAATCTTGCTAATGTCATTAACATTAAACTTACTAAAGTTGGGGTTGTTGGGGCCCTTGAAATCATTGAGGTTGCAAGAGCATCGGGATTGGATTTGATGATTGGTGGTATGGTTGAGACTAGACTCGCCATGGGCTTTTCTGGCCACCTTGCTGCTGGCCTTGGGTGTTTTAAGTTTATTGATCTAGATACGCCCCTATTGCTGTCAGAAGATCCAGTTCTGGAGGGTTATGAAGTTTCGGGTGCCATTTATAAGTTCAACAACGCTAGGGGCCATGGTGGATTTCTCCACTGGGACAACCTTGCTTGTACCAGTAATACCATAAAAGTGCTAATACCTGTATGA

Protein Analysis

266

Amino Acids

29.07

Weight (kDa)

6.07

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MR_MLE_C PF13378 54 - 245 3e-27 Enolase C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 708
AasI GACNNNNNNGTC 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 698
AciI CCGC 2 cut(s) 68, 142
AclWI GGATC 1 cut(s) 662
AcoI YGGCCR 2 cut(s) 16, 598
AcsI RAATTY 1 cut(s) 175
AfaI GTAC 1 cut(s) 766
AfiI CCNNNNNNNGG 4 cut(s) 52, 57, 58, 87
AgsI TTSAA 5 cut(s) 138, 197, 323, 515, 715
AluBI AGCT 6 cut(s) 98, 200, 258, 269, 407, 417
AluI AGCT 6 cut(s) 98, 200, 258, 269, 407, 417
Alw21I GWGCWC 1 cut(s) 419
Alw26I GTCTC 1 cut(s) 566
AlwI GGATC 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 52
AoxI GGCC 8 cut(s) 3, 16, 128, 352, 507, 598, 613, 727
ApaI GGGCCC 1 cut(s) 511
ApeKI GCWGC 3 cut(s) 95, 404, 608
ApoI RAATTY 1 cut(s) 175
Asp700I GAANNNNTTC 1 cut(s) 192
AspS9I GGNCC 4 cut(s) 129, 507, 508, 727
AsuC2I CCSGG 3 cut(s) 15, 53, 54
AvaI CYCGRG 1 cut(s) 52
BaeGI GKGCMC 1 cut(s) 511
BalI TGGCCA 1 cut(s) 600
BanI GGYRCC 1 cut(s) 698
BanII GRGCYC 2 cut(s) 419, 511
Bbv12I GWGCWC 1 cut(s) 419
BbvI GCAGC 3 cut(s) 107, 416, 595
BcgI CGANNNNNNTGC 2 cut(s) 519, 553
BcnI CCSGG 3 cut(s) 15, 53, 54
BcoDI GTCTC 1 cut(s) 566
BfaI CTAG 4 cut(s) 378, 576, 641, 723
BisI GCNGC 3 cut(s) 96, 405, 609
BlsI GCNGC 3 cut(s) 97, 406, 610
Bme1390I CCNGG 3 cut(s) 15, 53, 54
BmeT110I CYCGRG 1 cut(s) 52
BmgT120I GGNCC 4 cut(s) 129, 507, 508, 727
BmiI GGNNCC 5 cut(s) 35, 508, 509, 700, 728
BmrFI CCNGG 3 cut(s) 15, 53, 54
BmrI ACTGGG 1 cut(s) 757
BmsI GCATC 1 cut(s) 545
BmuI ACTGGG 1 cut(s) 757
BpmI CTGGAG 1 cut(s) 698
BpuEI CTTGAG 1 cut(s) 302
BpuMI CCSGG 3 cut(s) 15, 53, 54
BsaJI CCNNGG 8 cut(s) 13, 51, 52, 145, 355, 585, 616, 730
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 57, 58, 87
Bse1I ACTGG 5 cut(s) 311, 329, 671, 752, 768
BseDI CCNNGG 8 cut(s) 13, 51, 52, 145, 355, 585, 616, 730
BseGI GGATG 2 cut(s) 109, 439
BseLI CCNNNNNNNGG 4 cut(s) 52, 57, 58, 87
BseNI ACTGG 5 cut(s) 311, 329, 671, 752, 768
BseRI GAGGAG 1 cut(s) 90
BseSI GKGCMC 1 cut(s) 511
BseXI GCAGC 3 cut(s) 107, 416, 595
BshFI GGCC 8 cut(s) 5, 18, 130, 354, 509, 600, 615, 729
BshNI GGYRCC 1 cut(s) 698
BsiHKAI GWGCWC 1 cut(s) 419
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 2 cut(s) 15, 53
BslFI GGGAC 1 cut(s) 764
BslI CCNNNNNNNGG 4 cut(s) 52, 57, 58, 87
BsmAI GTCTC 1 cut(s) 566
BsmFI GGGAC 1 cut(s) 764
BsnI GGCC 8 cut(s) 5, 18, 130, 354, 509, 600, 615, 729
BsoBI CYCGRG 1 cut(s) 52
Bsp120I GGGCCC 1 cut(s) 507
Bsp1286I GDGCHC 2 cut(s) 419, 511
Bsp143I GATC 2 cut(s) 637, 667
Bsp19I CCATGG 2 cut(s) 585, 730
BspACI CCGC 2 cut(s) 68, 142
BspANI GGCC 8 cut(s) 5, 18, 130, 354, 509, 600, 615, 729
BspLI GGNNCC 5 cut(s) 35, 508, 509, 700, 728
BspPI GGATC 1 cut(s) 662
BspT107I GGYRCC 1 cut(s) 698
BsrI ACTGG 5 cut(s) 311, 329, 671, 752, 768
BssECI CCNNGG 8 cut(s) 13, 51, 52, 145, 355, 585, 616, 730
BssMI GATC 2 cut(s) 637, 667
BssT1I CCWWGG 5 cut(s) 145, 355, 585, 616, 730
Bst6I CTCTTC 1 cut(s) 122
BstC8I GCNNGC 2 cut(s) 93, 613
BstDSI CCRYGG 2 cut(s) 585, 730
BstF5I GGATG 2 cut(s) 109, 439
BstKTI GATC 2 cut(s) 640, 670
BstMAI GTCTC 1 cut(s) 566
BstMBI GATC 2 cut(s) 637, 667
BstMWI GCNNNNNNNGC 3 cut(s) 206, 597, 655
BstSCI CCNGG 3 cut(s) 13, 51, 52
BstSLI GKGCMC 1 cut(s) 511
BstV1I GCAGC 3 cut(s) 107, 416, 595
BstX2I RGATCY 1 cut(s) 667
BstYI RGATCY 1 cut(s) 667
BsuRI GGCC 8 cut(s) 5, 18, 130, 354, 509, 600, 615, 729
BtgI CCRYGG 2 cut(s) 585, 730
BtsCI GGATG 2 cut(s) 109, 439
BtsIMutI CAGTG 1 cut(s) 745
Cac8I GCNNGC 2 cut(s) 93, 613
Cfr13I GGNCC 4 cut(s) 129, 507, 508, 727
Cfr9I CCCGGG 1 cut(s) 52
CseI GACGC 1 cut(s) 248
Csp6I GTAC 1 cut(s) 765
CspCI CAANNNNNGTGG 2 cut(s) 203, 238
CviAII CATG 2 cut(s) 586, 731
CviQI GTAC 1 cut(s) 765
DpnI GATC 2 cut(s) 639, 669
DpnII GATC 2 cut(s) 637, 667
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 2 cut(s) 16, 598
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
EciI GGCGGA 2 cut(s) 57, 131
Ecl136II GAGCTC 1 cut(s) 417
Eco130I CCWWGG 5 cut(s) 145, 355, 585, 616, 730
Eco147I AGGCCT 1 cut(s) 354
Eco24I GRGCYC 2 cut(s) 419, 511
Eco53kI GAGCTC 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 52
EcoICRI GAGCTC 1 cut(s) 417
EcoO109I RGGNCCY 2 cut(s) 507, 508
EcoT14I CCWWGG 5 cut(s) 145, 355, 585, 616, 730
EcoT38I GRGCYC 2 cut(s) 419, 511
ErhI CCWWGG 5 cut(s) 145, 355, 585, 616, 730
FaeI CATG 2 cut(s) 589, 734
FalI AAGNNNNNCTT 2 cut(s) 444, 476
FaqI GGGAC 1 cut(s) 764
FatI CATG 2 cut(s) 585, 730
Fnu4HI GCNGC 3 cut(s) 96, 405, 609
FokI GGATG 2 cut(s) 96, 446
FriOI GRGCYC 2 cut(s) 419, 511
Fsp4HI GCNGC 3 cut(s) 96, 405, 609
FspBI CTAG 4 cut(s) 378, 576, 641, 723
GluI GCNGC 3 cut(s) 96, 405, 609
GsuI CTGGAG 1 cut(s) 698
HaeIII GGCC 8 cut(s) 5, 18, 130, 354, 509, 600, 615, 729
HapII CCGG 2 cut(s) 15, 53
HgaI GACGC 1 cut(s) 248
Hin1II CATG 2 cut(s) 589, 734
HinfI GANTC 4 cut(s) 28, 169, 307, 579
HpaII CCGG 2 cut(s) 15, 53
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 5 cut(s) 27, 33, 181, 220, 664
Hpy188III TCNNGA 5 cut(s) 281, 420, 540, 641, 677
Hpy8I GTNNAC 1 cut(s) 214
HpyAV CCTTC 2 cut(s) 203, 344
HpyCH4V TGCA 3 cut(s) 155, 404, 530
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 597, 655
Hsp92II CATG 2 cut(s) 589, 734
Kzo9I GATC 2 cut(s) 637, 667
LmnI GCTCC 3 cut(s) 33, 103, 263
Lsp1109I GCAGC 3 cut(s) 107, 416, 595
LweI GCATC 1 cut(s) 545
MaeI CTAG 4 cut(s) 378, 576, 641, 723
MaeIII GTNAC 1 cut(s) 364
MalI GATC 2 cut(s) 639, 669
MboI GATC 2 cut(s) 637, 667
MboII GAAGA 4 cut(s) 56, 109, 464, 677
MflI RGATCY 1 cut(s) 667
MhlI GDGCHC 2 cut(s) 419, 511
MlsI TGGCCA 1 cut(s) 600
MluCI AATT 3 cut(s) 133, 175, 287
MluNI TGGCCA 1 cut(s) 600
MlyI GAGTC 3 cut(s) 22, 316, 573
MmeI TCCRAC 2 cut(s) 64, 100
MnlI CCTC 9 cut(s) 16, 19, 47, 111, 123, 132, 289, 517, 673
Mox20I TGGCCA 1 cut(s) 600
MroXI GAANNNNTTC 1 cut(s) 192
MscI TGGCCA 1 cut(s) 600
MseI TTAA 4 cut(s) 438, 474, 480, 627
MslI CAYNNNNRTG 2 cut(s) 339, 782
Msp20I TGGCCA 1 cut(s) 600
MspA1I CMGCKG 1 cut(s) 407
MspI CCGG 2 cut(s) 15, 53
MspR9I CCNGG 3 cut(s) 15, 53, 54
MwoI GCNNNNNNNGC 3 cut(s) 206, 597, 655
NciI CCSGG 3 cut(s) 15, 53, 54
NcoI CCATGG 2 cut(s) 585, 730
NdeII GATC 2 cut(s) 637, 667
NlaIII CATG 2 cut(s) 589, 734
NlaIV GGNNCC 5 cut(s) 35, 508, 509, 700, 728
NmeAIII GCCGAG 1 cut(s) 67
PceI AGGCCT 1 cut(s) 354
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 1 cut(s) 169
PkrI GCNGC 3 cut(s) 97, 406, 610
PleI GAGTC 3 cut(s) 22, 315, 573
PpsI GAGTC 3 cut(s) 22, 315, 573
PsiI TTATAA 1 cut(s) 708
Psp124BI GAGCTC 1 cut(s) 419
PspN4I GGNNCC 5 cut(s) 35, 508, 509, 700, 728
PspOMI GGGCCC 1 cut(s) 507
PspPI GGNCC 4 cut(s) 129, 507, 508, 727
PsuI RGATCY 1 cut(s) 667
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 1 cut(s) 766
RsaNI GTAC 1 cut(s) 765
RseI CAYNNNNRTG 2 cut(s) 339, 782
SacI GAGCTC 1 cut(s) 419
SaqAI TTAA 4 cut(s) 438, 474, 480, 627
SatI GCNGC 3 cut(s) 96, 405, 609
Sau3AI GATC 2 cut(s) 637, 667
Sau96I GGNCC 4 cut(s) 129, 507, 508, 727
SchI GAGTC 3 cut(s) 22, 316, 573
ScrFI CCNGG 3 cut(s) 15, 53, 54
SduI GDGCHC 2 cut(s) 419, 511
SfaNI GCATC 1 cut(s) 545
SmaI CCCGGG 1 cut(s) 54
SmiMI CAYNNNNRTG 2 cut(s) 339, 782
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 3 cut(s) 133, 175, 287
SseBI AGGCCT 1 cut(s) 354
SsiI CCGC 2 cut(s) 68, 142
SspMI CTAG 4 cut(s) 378, 576, 641, 723
SstI GAGCTC 1 cut(s) 419
StuI AGGCCT 1 cut(s) 354
StyD4I CCNGG 3 cut(s) 13, 51, 52
StyI CCWWGG 5 cut(s) 145, 355, 585, 616, 730
TaqI TCGA 1 cut(s) 421
TasI AATT 3 cut(s) 133, 175, 287
TfiI GAWTC 1 cut(s) 169
Tru1I TTAA 4 cut(s) 438, 474, 480, 627
Tru9I TTAA 4 cut(s) 438, 474, 480, 627
TscAI CASTG 1 cut(s) 752
TseI GCWGC 3 cut(s) 95, 404, 608
TspDTI ATGAA 4 cut(s) 98, 279, 323, 702
TspMI CCCGGG 1 cut(s) 52
TspRI CASTG 1 cut(s) 752
XapI RAATTY 1 cut(s) 175
XbaI TCTAGA 1 cut(s) 640
XcmI CCANNNNNNNNNTGG 1 cut(s) 608
XmaI CCCGGG 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 192
XspI CTAG 4 cut(s) 378, 576, 641, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.