AT3G19850

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007978
Physical Location & Seq
Reverse (-)
6898163 .. 6901334
3172 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G19850.1

Sequence Viewer

Length: 1665 bp
ATGTCTCTTTGCTGTGATCTTCAAATCAATCTCAATAACCAATTTACTTTCTTCGTCAATCAGGATTTGATCTCAGAGTACTCAGGCTTCTTGAGGAAGATGATAAAACAGAGCAATAAGAAGAAAAAGAATCACAAGAACAGTAGAATCATCATCGAGGTTGAAGATTTTCCAGGTGGGTCAGATGGGTTCGACTTGGTTTTAAGATTCTGTTATGGTGGAGGAATCTCGATAGATGTCTCAAATGTGTCCATTTTGCATTGCTCTTCTGTCTTCCTTGAGATGACAGAGAAACTCTGTTCCTCGAATCTCTTGCTTCGAACAGAGAAGTTTCTAGAAGGAATGTTCTACTGGTCCTGGAACGACATCGTATTGTGTCTCAAGAGCTGCGAGCAAGTGTTCTTACACGCTGATTCTTACGGTCTTGTTGATAAGCTTGTTTTCGGGGTTTTAGCCAAAATCCCTCAGAATTCAGACGTGAGTCATGTCTTTTCATCATCCTCTCCGTCTTCCTCTGCCTCTGCCTCAGCCTCCTCTCAGTCGCCGGAGACGGCAATGATTAGGTCGTATTCAGACAAAAGGTCTACTTCGAGGTCTTTTTCTTGCAGGACAAGTAACGAGTGGTGGTTCGACGATATGTCAATTCTCGGGCCAAAAATCATTGAAAAGCTGATAAATACACTTGGTGCGCATGATAAGAACAATGACAGCTTGGTCCTCACAAAATTTCTTCTCCATTACCTCAAGACAAAGGTCCCAAACAAGTCAACCAACAAGCTCGAGTATTCAGGTTTAGCTGATACAGCGGTTCAAGGAGTGGTTTTCGCAGCGAAAACCGCGTTTTCATGCAGAAAAATGTTCTGGGTTCTGCGAGTTTTATCGGGATTTAGCATAAGTAAAGAATCAAGAATTGGTTTAGAGAGGGTTATAGGAGAAATGCTGGATCAAGCAACACTTGATGATCTTCTGATACCAGCTGGAGGAAAAGGAGAAAAAGGGGTTTACGATGTGGATTTGGTGATAAGATTACTCAAAGTGTTTGTAAGAATTGGAAACACAGAAGAAGGAGATCAGAATTTGAGAATGAGAAGAATTGGGAAGTTGATTGATAAGTATCTCAGAGAGATATCTCCAGACCAGAATCTTAAAGTGTCAAAGTTTCTTGAAGTTGCAGAGAGTTTGCCAGATTCAGCTAGAGATTGGTTTGATGGATTATACAGAGCCATTAACATCTATCTTGAGTCTCATCCGAAACTATCATCCGAGGATAGAACAAAACTATGTCGATGTCTAAACTACAAGAAATTGACATTGGACACATGCAAACAACTTGCAAAAAATCCCAAGATCCCTCCAAATATTGCAGTTCAAGCACTCAAGTCACAACAATTATCAAACGAGACTCGACCACACTCAAGAGAGGACAAGAACAAAGTAAACAAGATCTGGAATTCACGTAAGTACTTAGAAGAGAAACCAATACTGGTGTGTTTGAAAGGTTTTGATATGTCGGAGAAGTTTGAAGATGATCTAATGATGAATTTGGAGAGGAAGCAATGGAATAATTCTGAAAAAGTTAGTAAGGAGAAGAAGAGTGAAGTAATGTCAAGATCTGTGAGACATGGACATACACATTCAAGTTCTAGTTTTCCAAGGCTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

554

Amino Acids

62.99

Weight (kDa)

9.01

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 207 - 441 9.7e-65 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 713
Acc16I TGCGCA 1 cut(s) 690
AccI GTMKAC 1 cut(s) 584
AccII CGCG 1 cut(s) 839
AciI CCGC 2 cut(s) 806, 837
AclWI GGATC 2 cut(s) 951, 1342
AcsI RAATTY 5 cut(s) 469, 725, 1075, 1450, 1540
AdeI CACNNNGTG 1 cut(s) 686
AfaI GTAC 2 cut(s) 80, 1463
AfiI CCNNNNNNNGG 1 cut(s) 980
AgsI TTSAA 9 cut(s) 23, 164, 665, 812, 1166, 1370, 1495, 1523, 1638
AhdI GACNNNNNGTC 2 cut(s) 580, 637
AjiI CACGTC 1 cut(s) 478
AjnI CCWGG 2 cut(s) 172, 356
AjuI GAANNNNNNNTTGG 4 cut(s) 894, 926, 1295, 1327
AluBI AGCT 8 cut(s) 387, 436, 670, 711, 778, 797, 977, 1193
AluI AGCT 8 cut(s) 387, 436, 670, 711, 778, 797, 977, 1193
Alw26I GTCTC 7 cut(s) 9, 244, 383, 542, 1248, 1394, 1612
AlwI GGATC 2 cut(s) 951, 1342
Ama87I CYCGRG 2 cut(s) 647, 779
AoxI GGCC 1 cut(s) 650
ApeKI GCWGC 2 cut(s) 387, 827
ApoI RAATTY 5 cut(s) 469, 725, 1075, 1450, 1540
Asp700I GAANNNNTTC 1 cut(s) 168
AspLEI GCGC 1 cut(s) 691
AspS9I GGNCC 4 cut(s) 354, 650, 715, 754
AsuHPI GGTGA 1 cut(s) 1030
AsuII TTCGAA 1 cut(s) 319
AvaI CYCGRG 2 cut(s) 647, 779
AvaII GGWCC 3 cut(s) 354, 715, 754
BaeI ACNNNNGTAYC 2 cut(s) 792, 825
BarI GAAGNNNNNNTAC 2 cut(s) 71, 103
BbsI GAAGAC 2 cut(s) 265, 501
BbvCI CCTCAGC 1 cut(s) 526
BbvI GCAGC 2 cut(s) 374, 839
BccI CCATC 2 cut(s) 179, 1202
BceAI ACGGC 1 cut(s) 567
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 2 cut(s) 174, 358
BcoDI GTCTC 7 cut(s) 9, 244, 383, 542, 1248, 1394, 1612
BfaI CTAG 3 cut(s) 335, 1194, 1644
BglII AGATCT 2 cut(s) 1443, 1610
BisI GCNGC 2 cut(s) 388, 828
BlsI GCNGC 2 cut(s) 389, 829
BmcAI AGTACT 2 cut(s) 80, 1463
Bme1390I CCNGG 2 cut(s) 174, 358
Bme18I GGWCC 3 cut(s) 354, 715, 754
BmeRI GACNNNNNGTC 2 cut(s) 580, 637
BmeT110I CYCGRG 2 cut(s) 647, 779
BmgBI CACGTC 1 cut(s) 478
BmgT120I GGNCC 4 cut(s) 354, 650, 715, 754
BmiI GGNNCC 1 cut(s) 756
BmrFI CCNGG 2 cut(s) 174, 358
BoxI GACNNNNGTC 2 cut(s) 480, 752
BpiI GAAGAC 2 cut(s) 265, 501
BpmI CTGGAG 2 cut(s) 999, 1116
Bpu10I CCTNAGC 1 cut(s) 526
Bpu14I TTCGAA 1 cut(s) 319
BpuEI CTTGAG 7 cut(s) 112, 299, 365, 728, 1259, 1361, 1399
BsaAI YACGTR 1 cut(s) 1457
BsaBI GATNNNNATC 1 cut(s) 1113
BsaJI CCNNGG 2 cut(s) 1263, 1652
Bsc4I CCNNNNNNNGG 1 cut(s) 980
Bse1I ACTGG 2 cut(s) 356, 1488
Bse3DI GCAATG 3 cut(s) 259, 561, 1562
Bse8I GATNNNNATC 1 cut(s) 1113
BseBI CCWGG 2 cut(s) 174, 358
BseDI CCNNGG 2 cut(s) 1263, 1652
BseGI GGATG 3 cut(s) 497, 1246, 1259
BseJI GATNNNNATC 1 cut(s) 1113
BseLI CCNNNNNNNGG 1 cut(s) 980
BseMI GCAATG 3 cut(s) 259, 561, 1562
BseMII CTCAG 6 cut(s) 87, 96, 479, 540, 551, 1132
BseNI ACTGG 2 cut(s) 356, 1488
BseRI GAGGAG 1 cut(s) 523
BseXI GCAGC 2 cut(s) 374, 839
Bsh1236I CGCG 1 cut(s) 839
BshFI GGCC 1 cut(s) 652
BsiHKCI CYCGRG 2 cut(s) 647, 779
BsiSI CCGG 1 cut(s) 545
BslFI GGGAC 1 cut(s) 740
BslI CCNNNNNNNGG 1 cut(s) 980
BsmAI GTCTC 7 cut(s) 9, 244, 383, 542, 1248, 1394, 1612
BsmBI CGTCTC 1 cut(s) 542
BsmFI GGGAC 1 cut(s) 740
BsnI GGCC 1 cut(s) 652
BsoBI CYCGRG 2 cut(s) 647, 779
Bsp119I TTCGAA 1 cut(s) 319
Bsp143I GATC 9 cut(s) 16, 69, 943, 961, 1069, 1347, 1443, 1528, 1610
BspACI CCGC 2 cut(s) 806, 837
BspANI GGCC 1 cut(s) 652
BspCNI CTCAG 6 cut(s) 86, 95, 478, 539, 550, 1131
BspFNI CGCG 1 cut(s) 839
BspLI GGNNCC 1 cut(s) 756
BspPI GGATC 2 cut(s) 951, 1342
BspQI GCTCTTC 1 cut(s) 271
BspT104I TTCGAA 1 cut(s) 319
BsrDI GCAATG 3 cut(s) 259, 561, 1562
BsrI ACTGG 2 cut(s) 356, 1488
BssECI CCNNGG 2 cut(s) 1263, 1652
BssMI GATC 9 cut(s) 16, 69, 943, 961, 1069, 1347, 1443, 1528, 1610
BssT1I CCWWGG 1 cut(s) 1652
Bst2UI CCWGG 2 cut(s) 174, 358
Bst4CI ACNGT 2 cut(s) 143, 422
Bst6I CTCTTC 3 cut(s) 271, 1464, 1586
BstBAI YACGTR 1 cut(s) 1457
BstBI TTCGAA 1 cut(s) 319
BstC8I GCNNGC 1 cut(s) 392
BstDEI CTNAG 7 cut(s) 73, 82, 465, 526, 537, 1118, 1465
BstF5I GGATG 3 cut(s) 497, 1246, 1259
BstFNI CGCG 1 cut(s) 839
BstHHI GCGC 1 cut(s) 691
BstKTI GATC 9 cut(s) 19, 72, 946, 964, 1072, 1350, 1446, 1531, 1613
BstMAI GTCTC 7 cut(s) 9, 244, 383, 542, 1248, 1394, 1612
BstMBI GATC 9 cut(s) 16, 69, 943, 961, 1069, 1347, 1443, 1528, 1610
BstMWI GCNNNNNNNGC 3 cut(s) 803, 836, 1370
BstNI CCWGG 2 cut(s) 174, 358
BstNSI RCATGY 1 cut(s) 1323
BstPAI GACNNNNGTC 2 cut(s) 480, 752
BstSCI CCNGG 2 cut(s) 172, 356
BstUI CGCG 1 cut(s) 839
BstV1I GCAGC 2 cut(s) 374, 839
BstV2I GAAGAC 2 cut(s) 265, 501
BstX2I RGATCY 3 cut(s) 1347, 1443, 1610
BstYI RGATCY 3 cut(s) 1347, 1443, 1610
BsuRI GGCC 1 cut(s) 652
BtrI CACGTC 1 cut(s) 478
BtsCI GGATG 3 cut(s) 497, 1246, 1259
Cac8I GCNNGC 1 cut(s) 392
CfoI GCGC 1 cut(s) 691
Cfr13I GGNCC 4 cut(s) 354, 650, 715, 754
Csp6I GTAC 2 cut(s) 79, 1462
CviAII CATG 5 cut(s) 485, 692, 846, 1320, 1622
CviQI GTAC 2 cut(s) 79, 1462
DdeI CTNAG 7 cut(s) 73, 82, 465, 526, 537, 1118, 1465
DpnI GATC 9 cut(s) 18, 71, 945, 963, 1071, 1349, 1445, 1530, 1612
DpnII GATC 9 cut(s) 16, 69, 943, 961, 1069, 1347, 1443, 1528, 1610
DraIII CACNNNGTG 1 cut(s) 686
DrdI GACNNNNNNGTC 1 cut(s) 713
DriI GACNNNNNGTC 2 cut(s) 580, 637
DseDI GACNNNNNNGTC 1 cut(s) 713
Eam1104I CTCTTC 3 cut(s) 271, 1464, 1586
Eam1105I GACNNNNNGTC 2 cut(s) 580, 637
EarI CTCTTC 3 cut(s) 271, 1464, 1586
Eco130I CCWWGG 1 cut(s) 1652
Eco32I GATATC 1 cut(s) 1128
Eco47I GGWCC 3 cut(s) 354, 715, 754
Eco88I CYCGRG 2 cut(s) 647, 779
EcoO109I RGGNCCY 1 cut(s) 754
EcoRI GAATTC 2 cut(s) 469, 1450
EcoRII CCWGG 2 cut(s) 172, 356
EcoRV GATATC 1 cut(s) 1128
EcoT14I CCWWGG 1 cut(s) 1652
ErhI CCWWGG 1 cut(s) 1652
Esp3I CGTCTC 1 cut(s) 542
FaeI CATG 5 cut(s) 488, 695, 849, 1323, 1625
FalI AAGNNNNNCTT 4 cut(s) 571, 603, 939, 971
FaqI GGGAC 1 cut(s) 740
FatI CATG 5 cut(s) 484, 691, 845, 1319, 1621
FblI GTMKAC 1 cut(s) 584
Fnu4HI GCNGC 2 cut(s) 388, 828
FokI GGATG 3 cut(s) 484, 1233, 1246
Fsp4HI GCNGC 2 cut(s) 388, 828
FspAI RTGCGCAY 1 cut(s) 690
FspBI CTAG 3 cut(s) 335, 1194, 1644
FspI TGCGCA 1 cut(s) 690
GlaI GCGC 1 cut(s) 690
GluI GCNGC 2 cut(s) 388, 828
GsuI CTGGAG 2 cut(s) 999, 1116
HaeIII GGCC 1 cut(s) 652
HapII CCGG 1 cut(s) 545
HhaI GCGC 1 cut(s) 691
Hin1II CATG 5 cut(s) 488, 695, 849, 1323, 1625
Hin6I GCGC 1 cut(s) 689
HinP1I GCGC 1 cut(s) 689
HincII GTYRAC 1 cut(s) 768
HindII GTYRAC 1 cut(s) 768
HindIII AAGCTT 1 cut(s) 434
HpaII CCGG 1 cut(s) 545
HphI GGTGA 1 cut(s) 1030
Hpy166II GTNNAC 4 cut(s) 585, 768, 1003, 1438
Hpy8I GTNNAC 4 cut(s) 585, 768, 1003, 1438
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 2 cut(s) 332, 1058
HpyCH4III ACNGT 2 cut(s) 143, 422
HpyCH4IV ACGT 2 cut(s) 477, 1456
HpyCH4V TGCA 7 cut(s) 259, 606, 849, 1172, 1323, 1334, 1364
HpyF10VI GCNNNNNNNGC 3 cut(s) 803, 836, 1370
HpyF3I CTNAG 7 cut(s) 73, 82, 465, 526, 537, 1118, 1465
HpySE526I ACGT 2 cut(s) 477, 1456
Hsp92II CATG 5 cut(s) 488, 695, 849, 1323, 1625
HspAI GCGC 1 cut(s) 689
Kzo9I GATC 9 cut(s) 16, 69, 943, 961, 1069, 1347, 1443, 1528, 1610
LguI GCTCTTC 1 cut(s) 271
Lsp1109I GCAGC 2 cut(s) 374, 839
MaeI CTAG 3 cut(s) 335, 1194, 1644
MaeII ACGT 2 cut(s) 477, 1456
MaeIII GTNAC 2 cut(s) 614, 1380
MalI GATC 9 cut(s) 18, 71, 945, 963, 1071, 1349, 1445, 1530, 1612
MboI GATC 9 cut(s) 16, 69, 943, 961, 1069, 1347, 1443, 1528, 1610
MflI RGATCY 3 cut(s) 1347, 1443, 1610
MlyI GAGTC 3 cut(s) 490, 1250, 1396
MmeI TCCRAC 1 cut(s) 1491
MroXI GAANNNNTTC 1 cut(s) 168
MseI TTAA 4 cut(s) 203, 1146, 1227, 1663
MspA1I CMGCKG 2 cut(s) 806, 977
MspI CCGG 1 cut(s) 545
MspR9I CCNGG 2 cut(s) 174, 358
MvaI CCWGG 2 cut(s) 174, 358
MvnI CGCG 1 cut(s) 839
MwoI GCNNNNNNNGC 3 cut(s) 803, 836, 1370
NdeII GATC 9 cut(s) 16, 69, 943, 961, 1069, 1347, 1443, 1528, 1610
NlaIII CATG 5 cut(s) 488, 695, 849, 1323, 1625
NlaIV GGNNCC 1 cut(s) 756
NmuCI GTSAC 1 cut(s) 1380
NsbI TGCGCA 1 cut(s) 690
NspI RCATGY 1 cut(s) 1323
NspV TTCGAA 1 cut(s) 319
PaeR7I CTCGAG 1 cut(s) 779
PciSI GCTCTTC 1 cut(s) 271
PdmI GAANNNNTTC 1 cut(s) 168
PfeI GAWTC 9 cut(s) 130, 147, 207, 225, 307, 413, 902, 1141, 1187
PfoI TCCNGGA 1 cut(s) 356
PkrI GCNGC 2 cut(s) 389, 829
PleI GAGTC 3 cut(s) 489, 1249, 1396
PpsI GAGTC 3 cut(s) 489, 1249, 1396
Ppu21I YACGTR 1 cut(s) 1457
PpuMI RGGWCCY 1 cut(s) 754
PshAI GACNNNNGTC 2 cut(s) 480, 752
Psp5II RGGWCCY 1 cut(s) 754
Psp6I CCWGG 2 cut(s) 172, 356
PspGI CCWGG 2 cut(s) 172, 356
PspN4I GGNNCC 1 cut(s) 756
PspPI GGNCC 4 cut(s) 354, 650, 715, 754
PspPPI RGGWCCY 1 cut(s) 754
PspXI VCTCGAGB 1 cut(s) 779
PsuI RGATCY 3 cut(s) 1347, 1443, 1610
PvuII CAGCTG 1 cut(s) 977
RsaI GTAC 2 cut(s) 80, 1463
RsaNI GTAC 2 cut(s) 79, 1462
SapI GCTCTTC 1 cut(s) 271
SaqAI TTAA 4 cut(s) 203, 1146, 1227, 1663
SatI GCNGC 2 cut(s) 388, 828
Sau3AI GATC 9 cut(s) 16, 69, 943, 961, 1069, 1347, 1443, 1528, 1610
Sau96I GGNCC 4 cut(s) 354, 650, 715, 754
ScaI AGTACT 2 cut(s) 80, 1463
SchI GAGTC 3 cut(s) 490, 1250, 1396
ScrFI CCNGG 2 cut(s) 174, 358
Sfr274I CTCGAG 1 cut(s) 779
SfuI TTCGAA 1 cut(s) 319
SinI GGWCC 3 cut(s) 354, 715, 754
SlaI CTCGAG 1 cut(s) 779
SmlI CTYRAG 8 cut(s) 91, 278, 380, 743, 779, 1238, 1376, 1414
SmoI CTYRAG 8 cut(s) 91, 278, 380, 743, 779, 1238, 1376, 1414
SsiI CCGC 2 cut(s) 806, 837
SspI AATATT 1 cut(s) 1360
SspMI CTAG 3 cut(s) 335, 1194, 1644
StyD4I CCNGG 2 cut(s) 172, 356
StyI CCWWGG 1 cut(s) 1652
TaaI ACNGT 2 cut(s) 143, 422
TaiI ACGT 2 cut(s) 480, 1459
TatI WGTACW 2 cut(s) 78, 1461
TfiI GAWTC 9 cut(s) 130, 147, 207, 225, 307, 413, 902, 1141, 1187
Tru1I TTAA 4 cut(s) 203, 1146, 1227, 1663
Tru9I TTAA 4 cut(s) 203, 1146, 1227, 1663
TseFI GTSAC 1 cut(s) 1380
TseI GCWGC 2 cut(s) 387, 827
Tsp45I GTSAC 1 cut(s) 1380
TspDTI ATGAA 3 cut(s) 483, 834, 1553
TspGWI ACGGA 1 cut(s) 495
VpaK11BI GGWCC 3 cut(s) 354, 715, 754
XapI RAATTY 5 cut(s) 469, 725, 1075, 1450, 1540
XbaI TCTAGA 1 cut(s) 334
XceI RCATGY 1 cut(s) 1323
XhoI CTCGAG 1 cut(s) 779
XmiI GTMKAC 1 cut(s) 584
XmnI GAANNNNTTC 1 cut(s) 168
XspI CTAG 3 cut(s) 335, 1194, 1644
ZrmI AGTACT 2 cut(s) 80, 1463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.