pycom16g00540

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0007978
Physical Location & Seq
Forward (+)
335549 .. 336589
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g00540.1

Sequence Viewer

Length: 945 bp
ATGCAAATAGCAGCAGCAGCAGCTGCAGCGGCCATGCCTCACCTCTCTGATTTACAAATCTACATTGGTCAGCAAACATTCTTTGTCAATGAGGAGATTTTATCAAAATATTCATCAAGGTTGAAGAAGATGATCAAGGAAGAAAGGAGAAGAACCCAGATCAAGAATTCCGGGATCAAAATCGATGACTTTCCGGGAGGCCCTGACGGGTTCGAGTTAGTTTCTCGATTCTGTTACGACAACGGCAGAATCACACTCACGGTTTCAAACGTGTGCCTACTCCACTGCTCTGCAATCTTCCTTGGCATGACCGAAAAGCTTTCGCCCCGCAATTTGTTGCAGAAAACAAGTCATTTTCTTGAGGGACTTTTCGAGTGGTCGTTCAAAGACGTACTAGTCTGCCTCAAGAGCTGCCTCTCGTTCTTCGACCACGCAGACTCGTCCGGTCTTTTAGACAAACTCATTACTGCACTGCTTGCAAAGATCGCTCAGAATTCTGACGTGAGCAACCTCATTGACACGTCGCCTTCGTCTTCGTCTTCTCCGGAAACTGCATCTGGGTTCAGACTCTCTTCCACCCCGGAATCCATCAGGCCGAGCTCGTCCAGCAGAGCGTGGTGGTTCGATGACGTGGCCCTTCTGCCACCAAATATCATTGAGAAACTCCTCCAGATTTTAGGTGCTTACGGACCCGAAAACAATAGATCGATCCTCACCAGATTCCTCCTCCACTACCTGAAAATCTCTGCCCAGAAAAAACCCGGGAACTCGAAATGCGAGCTCGGAGGGCTCGCCGACACGGCGGTTCACGGCGTGATCTTGGTGGGCAAAAAGGTCTTTTCGTGCAGAGCTCTGTTCTGGGTTCTGAGGATTGTGTCTGGATTTGGAATTAGCAAAGAGTACAAAGTCGGGTTGGAGAGATTGATTGGTGGGATCGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

315

Amino Acids

34.65

Weight (kDa)

9.0

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 206 - 310 1.2e-07 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
AccIII TCCGGA 1 cut(s) 544
AciI CCGC 3 cut(s) 29, 328, 803
AclWI GGATC 3 cut(s) 182, 703, 941
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 2 cut(s) 166, 493
AdeI CACNNNGTG 1 cut(s) 814
AfaI GTAC 2 cut(s) 393, 902
AflIII ACRYGT 2 cut(s) 270, 519
AgsI TTSAA 3 cut(s) 124, 267, 385
AhlI ACTAGT 1 cut(s) 394
AjiI CACGTC 3 cut(s) 502, 522, 631
AluBI AGCT 6 cut(s) 23, 319, 411, 600, 781, 851
AluI AGCT 6 cut(s) 23, 319, 411, 600, 781, 851
Alw21I GWGCWC 3 cut(s) 602, 783, 853
AlwI GGATC 3 cut(s) 182, 703, 941
AlwNI CAGNNNCTG 1 cut(s) 23
Ama87I CYCGRG 1 cut(s) 761
Aor13HI TCCGGA 1 cut(s) 544
AoxI GGCC 4 cut(s) 30, 199, 593, 633
ApeKI GCWGC 7 cut(s) 11, 14, 17, 20, 23, 26, 411
ApoI RAATTY 2 cut(s) 166, 493
AspS9I GGNCC 3 cut(s) 200, 634, 689
AsuC2I CCSGG 5 cut(s) 172, 195, 581, 762, 763
AsuHPI GGTGA 2 cut(s) 32, 706
AvaI CYCGRG 1 cut(s) 761
AvaII GGWCC 1 cut(s) 689
BanII GRGCYC 4 cut(s) 602, 783, 792, 853
BbsI GAAGAC 2 cut(s) 525, 531
Bbv12I GWGCWC 3 cut(s) 602, 783, 853
BbvI GCAGC 7 cut(s) 10, 23, 26, 29, 32, 38, 398
BccI CCATC 1 cut(s) 596
BceAI ACGGC 3 cut(s) 259, 816, 826
BclI TGATCA 1 cut(s) 132
BcnI CCSGG 5 cut(s) 172, 195, 581, 762, 763
BcuI ACTAGT 1 cut(s) 394
BfaI CTAG 1 cut(s) 395
BfmI CTRYAG 1 cut(s) 24
BglI GCCNNNNNGGC 1 cut(s) 800
BisI GCNGC 8 cut(s) 12, 15, 18, 21, 24, 27, 30, 412
BlsI GCNGC 8 cut(s) 13, 16, 19, 22, 25, 28, 31, 413
Bme1390I CCNGG 5 cut(s) 172, 195, 581, 762, 763
Bme18I GGWCC 1 cut(s) 689
BmeT110I CYCGRG 1 cut(s) 761
BmgBI CACGTC 3 cut(s) 502, 522, 631
BmgT120I GGNCC 3 cut(s) 200, 634, 689
BmiI GGNNCC 1 cut(s) 691
BmrFI CCNGG 5 cut(s) 172, 195, 581, 762, 763
BmsI GCATC 1 cut(s) 563
BpiI GAAGAC 2 cut(s) 525, 531
BpmI CTGGAG 1 cut(s) 653
BpuEI CTTGAG 2 cut(s) 380, 389
BpuMI CCSGG 5 cut(s) 172, 195, 581, 762, 763
Bsa29I ATCGAT 4 cut(s) 183, 707, 936, 940
BsaBI GATNNNNATC 1 cut(s) 179
BsaJI CCNNGG 3 cut(s) 301, 579, 761
BsaWI WCCGGW 2 cut(s) 443, 544
Bse8I GATNNNNATC 1 cut(s) 179
BseAI TCCGGA 1 cut(s) 544
BseCI ATCGAT 4 cut(s) 183, 707, 936, 940
BseDI CCNNGG 3 cut(s) 301, 579, 761
BseJI GATNNNNATC 1 cut(s) 179
BseMII CTCAG 2 cut(s) 503, 857
BseRI GAGGAG 3 cut(s) 107, 656, 716
BseXI GCAGC 7 cut(s) 10, 23, 26, 29, 32, 38, 398
BsgI GTGCAG 2 cut(s) 453, 865
Bsh1285I CGRYCG 1 cut(s) 940
BshFI GGCC 4 cut(s) 32, 201, 595, 635
BshVI ATCGAT 4 cut(s) 183, 707, 936, 940
BsiEI CGRYCG 1 cut(s) 940
BsiHKAI GWGCWC 3 cut(s) 602, 783, 853
BsiHKCI CYCGRG 1 cut(s) 761
BsiSI CCGG 6 cut(s) 171, 194, 444, 545, 581, 762
BslFI GGGAC 1 cut(s) 378
BsmFI GGGAC 1 cut(s) 378
BsnI GGCC 4 cut(s) 32, 201, 595, 635
BsoBI CYCGRG 1 cut(s) 761
Bsp1286I GDGCHC 4 cut(s) 602, 783, 792, 853
Bsp13I TCCGGA 1 cut(s) 544
Bsp143I GATC 9 cut(s) 132, 159, 174, 483, 704, 708, 816, 933, 937
BspACI CCGC 3 cut(s) 29, 328, 803
BspANI GGCC 4 cut(s) 32, 201, 595, 635
BspCNI CTCAG 2 cut(s) 502, 858
BspDI ATCGAT 4 cut(s) 183, 707, 936, 940
BspEI TCCGGA 1 cut(s) 544
BspLI GGNNCC 1 cut(s) 691
BspMAI CTGCAG 1 cut(s) 28
BspPI GGATC 3 cut(s) 182, 703, 941
BssECI CCNNGG 3 cut(s) 301, 579, 761
BssMI GATC 9 cut(s) 132, 159, 174, 483, 704, 708, 816, 933, 937
BssT1I CCWWGG 1 cut(s) 301
Bst4CI ACNGT 1 cut(s) 262
Bst6I CTCTTC 1 cut(s) 577
BstAPI GCANNNNNTGC 2 cut(s) 23, 476
BstC8I GCNNGC 3 cut(s) 477, 779, 792
BstDEI CTNAG 2 cut(s) 489, 866
BstKTI GATC 9 cut(s) 135, 162, 177, 486, 707, 711, 819, 936, 940
BstMBI GATC 9 cut(s) 132, 159, 174, 483, 704, 708, 816, 933, 937
BstMCI CGRYCG 1 cut(s) 940
BstSCI CCNGG 5 cut(s) 170, 193, 579, 760, 761
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 7 cut(s) 10, 23, 26, 29, 32, 38, 398
BstV2I GAAGAC 2 cut(s) 525, 531
Bsu15I ATCGAT 4 cut(s) 183, 707, 936, 940
BsuRI GGCC 4 cut(s) 32, 201, 595, 635
BsuTUI ATCGAT 4 cut(s) 183, 707, 936, 940
BtrI CACGTC 3 cut(s) 502, 522, 631
BtsI GCAGTG 2 cut(s) 283, 470
BtsIMutI CAGTG 2 cut(s) 283, 470
Cac8I GCNNGC 3 cut(s) 477, 779, 792
CaiI CAGNNNCTG 1 cut(s) 23
Cfr13I GGNCC 3 cut(s) 200, 634, 689
Cfr9I CCCGGG 1 cut(s) 761
ClaI ATCGAT 4 cut(s) 183, 707, 936, 940
Csp6I GTAC 2 cut(s) 392, 901
CviAII CATG 2 cut(s) 34, 307
CviQI GTAC 2 cut(s) 392, 901
DdeI CTNAG 2 cut(s) 489, 866
DpnI GATC 9 cut(s) 134, 161, 176, 485, 706, 710, 818, 935, 939
DpnII GATC 9 cut(s) 132, 159, 174, 483, 704, 708, 816, 933, 937
DraIII CACNNNGTG 1 cut(s) 814
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 1 cut(s) 577
EarI CTCTTC 1 cut(s) 577
Ecl136II GAGCTC 3 cut(s) 600, 781, 851
Eco130I CCWWGG 1 cut(s) 301
Eco24I GRGCYC 4 cut(s) 602, 783, 792, 853
Eco47I GGWCC 1 cut(s) 689
Eco53kI GAGCTC 3 cut(s) 600, 781, 851
Eco88I CYCGRG 1 cut(s) 761
EcoICRI GAGCTC 3 cut(s) 600, 781, 851
EcoO109I RGGNCCY 1 cut(s) 200
EcoRI GAATTC 2 cut(s) 166, 493
EcoT14I CCWWGG 1 cut(s) 301
EcoT38I GRGCYC 4 cut(s) 602, 783, 792, 853
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 2 cut(s) 37, 310
FaiI YATR 2 cut(s) 35, 308
FaqI GGGAC 1 cut(s) 378
FatI CATG 2 cut(s) 33, 306
FauI CCCGC 1 cut(s) 335
FbaI TGATCA 1 cut(s) 132
Fnu4HI GCNGC 8 cut(s) 12, 15, 18, 21, 24, 27, 30, 412
FriOI GRGCYC 4 cut(s) 602, 783, 792, 853
Fsp4HI GCNGC 8 cut(s) 12, 15, 18, 21, 24, 27, 30, 412
FspBI CTAG 1 cut(s) 395
GluI GCNGC 8 cut(s) 12, 15, 18, 21, 24, 27, 30, 412
GsuI CTGGAG 1 cut(s) 653
HaeIII GGCC 4 cut(s) 32, 201, 595, 635
HapII CCGG 6 cut(s) 171, 194, 444, 545, 581, 762
Hin1II CATG 2 cut(s) 37, 310
HindIII AAGCTT 1 cut(s) 317
HinfI GANTC 6 cut(s) 228, 249, 437, 567, 584, 720
HpaII CCGG 6 cut(s) 171, 194, 444, 545, 581, 762
HphI GGTGA 2 cut(s) 32, 706
Hpy166II GTNNAC 1 cut(s) 808
Hpy188I TCNGA 6 cut(s) 49, 492, 499, 566, 785, 867
Hpy188III TCNNGA 7 cut(s) 163, 225, 359, 406, 545, 670, 879
Hpy8I GTNNAC 1 cut(s) 808
Hpy99I CGWCG 1 cut(s) 526
HpyAV CCTTC 2 cut(s) 537, 647
HpyCH4III ACNGT 1 cut(s) 262
HpyCH4IV ACGT 5 cut(s) 270, 390, 501, 521, 630
HpyCH4V TGCA 8 cut(s) 4, 26, 293, 340, 470, 479, 554, 846
HpyF3I CTNAG 2 cut(s) 489, 866
HpySE526I ACGT 5 cut(s) 270, 390, 501, 521, 630
Hsp92II CATG 2 cut(s) 37, 310
Kpn2I TCCGGA 1 cut(s) 544
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 9 cut(s) 132, 159, 174, 483, 704, 708, 816, 933, 937
Lsp1109I GCAGC 7 cut(s) 10, 23, 26, 29, 32, 38, 398
LweI GCATC 1 cut(s) 563
MaeI CTAG 1 cut(s) 395
MaeII ACGT 5 cut(s) 270, 390, 501, 521, 630
MaeIII GTNAC 1 cut(s) 233
MalI GATC 9 cut(s) 134, 161, 176, 485, 706, 710, 818, 935, 939
MboI GATC 9 cut(s) 132, 159, 174, 483, 704, 708, 816, 933, 937
MboII GAAGA 9 cut(s) 136, 139, 152, 162, 289, 415, 525, 531, 564
MhlI GDGCHC 4 cut(s) 602, 783, 792, 853
MluCI AATT 4 cut(s) 166, 331, 493, 888
MlyI GAGTC 2 cut(s) 431, 561
MmeI TCCRAC 1 cut(s) 894
MroI TCCGGA 1 cut(s) 544
MspA1I CMGCKG 2 cut(s) 23, 29
MspI CCGG 6 cut(s) 171, 194, 444, 545, 581, 762
MspR9I CCNGG 5 cut(s) 172, 195, 581, 762, 763
NciI CCSGG 5 cut(s) 172, 195, 581, 762, 763
NdeII GATC 9 cut(s) 132, 159, 174, 483, 704, 708, 816, 933, 937
NlaIII CATG 2 cut(s) 37, 310
NlaIV GGNNCC 1 cut(s) 691
NmeAIII GCCGAG 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 527
PfeI GAWTC 4 cut(s) 228, 249, 584, 720
PfoI TCCNGGA 2 cut(s) 170, 193
PkrI GCNGC 8 cut(s) 13, 16, 19, 22, 25, 28, 31, 413
Ple19I CGATCG 1 cut(s) 940
PleI GAGTC 2 cut(s) 431, 561
PpsI GAGTC 2 cut(s) 431, 561
Psp124BI GAGCTC 3 cut(s) 602, 783, 853
PspN4I GGNNCC 1 cut(s) 691
PspPI GGNCC 3 cut(s) 200, 634, 689
PstI CTGCAG 1 cut(s) 28
PstNI CAGNNNCTG 1 cut(s) 23
PvuI CGATCG 1 cut(s) 940
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 2 cut(s) 393, 902
RsaNI GTAC 2 cut(s) 392, 901
SacI GAGCTC 3 cut(s) 602, 783, 853
SatI GCNGC 8 cut(s) 12, 15, 18, 21, 24, 27, 30, 412
Sau3AI GATC 9 cut(s) 132, 159, 174, 483, 704, 708, 816, 933, 937
Sau96I GGNCC 3 cut(s) 200, 634, 689
SchI GAGTC 2 cut(s) 431, 561
ScrFI CCNGG 5 cut(s) 172, 195, 581, 762, 763
SduI GDGCHC 4 cut(s) 602, 783, 792, 853
SfaNI GCATC 1 cut(s) 563
SfcI CTRYAG 1 cut(s) 24
SinI GGWCC 1 cut(s) 689
SmaI CCCGGG 1 cut(s) 763
SmlI CTYRAG 2 cut(s) 359, 404
SmoI CTYRAG 2 cut(s) 359, 404
SpeI ACTAGT 1 cut(s) 394
Sse9I AATT 4 cut(s) 166, 331, 493, 888
SsiI CCGC 3 cut(s) 29, 328, 803
SspI AATATT 1 cut(s) 110
SspMI CTAG 1 cut(s) 395
SstI GAGCTC 3 cut(s) 602, 783, 853
StyD4I CCNGG 5 cut(s) 170, 193, 579, 760, 761
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 1 cut(s) 262
TaiI ACGT 5 cut(s) 273, 393, 504, 524, 633
TaqII GACCGA 1 cut(s) 326
TasI AATT 4 cut(s) 166, 331, 493, 888
TatI WGTACW 1 cut(s) 900
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 4 cut(s) 228, 249, 584, 720
TscAI CASTG 2 cut(s) 290, 477
TseI GCWGC 7 cut(s) 11, 14, 17, 20, 23, 26, 411
TspDTI ATGAA 1 cut(s) 102
TspGWI ACGGA 1 cut(s) 702
TspMI CCCGGG 1 cut(s) 761
TspRI CASTG 2 cut(s) 290, 477
VpaK11BI GGWCC 1 cut(s) 689
XapI RAATTY 2 cut(s) 166, 493
XmaI CCCGGG 1 cut(s) 761
XspI CTAG 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.