Rorug04G0371100

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007978
Physical Location & Seq
Forward (+)
54244923 .. 54245588
666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0371100.1

Sequence Viewer

Length: 666 bp
ATGGCCGATGCCAGCGAGTTACCAGAGATTGCAATCTGCCAAATCTTCCAATCGCTTTCCACTAAGTTTGCAGTCCGGGCCAGCATTCTTTCAAAGCAATGGGAACGTGTCTGGCCATTAGTCCCGGTATTAGATTTTGACGAGGACGAGAAGGTACACAATGCTCACAATGCCAGCCCTTGTCGACACAGAAAGTTCCTGGAGTTTGTAACACTGTGTTTGAGGCGTCGCGAAGAAGATAAGTTGTTACATAAATTCCGGCTTTGCATGGAGTACTGTTATTACTGTAAAGACTGTGGAGAGCTCGTGGATAAGTGTTTGAGTTTTGCTCTTGACAGAAATGTTAAAGAGTTGCATGTTGCTAAATCAGGCTACTTCTTATCACATAAGGTTCTGAATGCTGAATATTTAGCTGTTTTAAGTTTGGATTCTATTGAAATAAGGGATGATGATATTGTAACTCTTCCGTCTTTGAAATCTTTGTCTCTAATTTGTATAAGTTTTGGTGGCTTGGGATTCCTCCACCTTATGGCAGGCTGTCCTTCCCTCGAGAATTTGTCAGTGGTTAAATGTAGAGGCTTCGATGAAAGAATTGAAGTTTCAAGTTCGAGCTTCAAATCCTTAAAAGTTAAAAGTTTTCAACTCGGGTGTTGCACTCCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

221

Amino Acids

25.11

Weight (kDa)

6.65

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 106 - 211 1.4e-08 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 529
AccI GTMKAC 1 cut(s) 184
AccII CGCG 1 cut(s) 231
AcoI YGGCCR 2 cut(s) 3, 113
AcsI RAATTY 2 cut(s) 254, 553
AcyI GRCGYC 1 cut(s) 226
AfaI GTAC 2 cut(s) 156, 275
AfiI CCNNNNNNNGG 1 cut(s) 529
AflIII ACRYGT 1 cut(s) 106
AgsI TTSAA 7 cut(s) 93, 437, 475, 596, 603, 616, 641
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 3 cut(s) 304, 413, 612
AluI AGCT 3 cut(s) 304, 413, 612
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 489
Ama87I CYCGRG 2 cut(s) 548, 644
AoxI GGCC 3 cut(s) 3, 78, 113
ApoI RAATTY 2 cut(s) 254, 553
AspS9I GGNCC 1 cut(s) 78
AsuC2I CCSGG 2 cut(s) 77, 125
AvaI CYCGRG 2 cut(s) 548, 644
BalI TGGCCA 1 cut(s) 115
BanII GRGCYC 1 cut(s) 306
BauI CACGAG 1 cut(s) 305
Bbv12I GWGCWC 1 cut(s) 306
BciT130I CCWGG 1 cut(s) 200
BcnI CCSGG 2 cut(s) 77, 125
BcoDI GTCTC 1 cut(s) 489
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 3 cut(s) 77, 125, 200
BmeT110I CYCGRG 2 cut(s) 548, 644
BmgT120I GGNCC 1 cut(s) 78
BmrFI CCNGG 3 cut(s) 77, 125, 200
BplI GAGNNNNNCTC 2 cut(s) 313, 345
BpmI CTGGAG 1 cut(s) 221
BpuMI CCSGG 2 cut(s) 77, 125
BsaBI GATNNNNATC 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 226
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse3DI GCAATG 1 cut(s) 104
Bse8I GATNNNNATC 1 cut(s) 32
BseBI CCWGG 1 cut(s) 200
BseGI GGATG 1 cut(s) 451
BseJI GATNNNNATC 1 cut(s) 32
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMI GCAATG 1 cut(s) 104
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 3 cut(s) 5, 80, 115
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 2 cut(s) 548, 644
BsiSI CCGG 3 cut(s) 76, 125, 259
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 1 cut(s) 529
BsmAI GTCTC 1 cut(s) 489
BsmFI GGGAC 1 cut(s) 107
BsmI GAATGC 2 cut(s) 84, 403
BsnI GGCC 3 cut(s) 5, 80, 115
BsoBI CYCGRG 2 cut(s) 548, 644
Bsp1286I GDGCHC 1 cut(s) 306
Bsp68I TCGCGA 1 cut(s) 231
BspANI GGCC 3 cut(s) 5, 80, 115
BspFNI CGCG 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 104
BssNI GRCGYC 1 cut(s) 226
BssSI CACGAG 1 cut(s) 305
Bst2BI CACGAG 1 cut(s) 305
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 4 cut(s) 216, 278, 287, 296
Bst6I CTCTTC 1 cut(s) 468
BstACI GRCGYC 1 cut(s) 226
BstC8I GCNNGC 4 cut(s) 13, 82, 175, 535
BstDEI CTNAG 1 cut(s) 63
BstF5I GGATG 1 cut(s) 451
BstFNI CGCG 1 cut(s) 231
BstMAI GTCTC 1 cut(s) 489
BstMWI GCNNNNNNNGC 2 cut(s) 77, 170
BstNI CCWGG 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 359
BstSCI CCNGG 3 cut(s) 75, 123, 198
BstUI CGCG 1 cut(s) 231
BsuRI GGCC 3 cut(s) 5, 80, 115
BtsCI GGATG 1 cut(s) 451
BtsIMutI CAGTG 2 cut(s) 212, 567
BtuMI TCGCGA 1 cut(s) 231
Cac8I GCNNGC 4 cut(s) 13, 82, 175, 535
Cfr13I GGNCC 1 cut(s) 78
CseI GACGC 1 cut(s) 215
Csp6I GTAC 2 cut(s) 155, 274
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 2 cut(s) 268, 356
CviQI GTAC 2 cut(s) 155, 274
DdeI CTNAG 1 cut(s) 63
EaeI YGGCCR 2 cut(s) 3, 113
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
Ecl136II GAGCTC 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 306
Eco53kI GAGCTC 1 cut(s) 304
Eco88I CYCGRG 2 cut(s) 548, 644
EcoICRI GAGCTC 1 cut(s) 304
EcoRII CCWGG 1 cut(s) 198
EcoT38I GRGCYC 1 cut(s) 306
FaeI CATG 2 cut(s) 271, 359
FaiI YATR 6 cut(s) 252, 269, 357, 387, 497, 530
FaqI GGGAC 1 cut(s) 107
FatI CATG 2 cut(s) 267, 355
FblI GTMKAC 1 cut(s) 184
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 1 cut(s) 306
GsuI CTGGAG 1 cut(s) 221
HaeIII GGCC 3 cut(s) 5, 80, 115
HapII CCGG 3 cut(s) 76, 125, 259
HgaI GACGC 1 cut(s) 215
Hin1I GRCGYC 1 cut(s) 226
Hin1II CATG 2 cut(s) 271, 359
HincII GTYRAC 1 cut(s) 185
HindII GTYRAC 1 cut(s) 185
HinfI GANTC 2 cut(s) 428, 516
HpaII CCGG 3 cut(s) 76, 125, 259
Hpy166II GTNNAC 2 cut(s) 157, 185
Hpy188I TCNGA 1 cut(s) 396
Hpy188III TCNNGA 3 cut(s) 230, 332, 550
Hpy8I GTNNAC 2 cut(s) 157, 185
Hpy99I CGWCG 1 cut(s) 231
HpyAV CCTTC 2 cut(s) 145, 552
HpyCH4III ACNGT 4 cut(s) 216, 278, 287, 296
HpyCH4IV ACGT 1 cut(s) 106
HpyCH4V TGCA 5 cut(s) 32, 71, 267, 355, 654
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 170
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 1 cut(s) 106
Hsp92I GRCGYC 1 cut(s) 226
Hsp92II CATG 2 cut(s) 271, 359
MaeII ACGT 1 cut(s) 106
MaeIII GTNAC 4 cut(s) 18, 208, 246, 457
MboII GAAGA 4 cut(s) 37, 245, 248, 455
MhlI GDGCHC 1 cut(s) 306
MlsI TGGCCA 1 cut(s) 115
MluCI AATT 4 cut(s) 254, 489, 553, 591
MluNI TGGCCA 1 cut(s) 115
MnlI CCTC 5 cut(s) 136, 216, 530, 557, 569
Mox20I TGGCCA 1 cut(s) 115
MscI TGGCCA 1 cut(s) 115
MseI TTAA 5 cut(s) 345, 419, 567, 623, 630
Msp20I TGGCCA 1 cut(s) 115
MspI CCGG 3 cut(s) 76, 125, 259
MspR9I CCNGG 3 cut(s) 77, 125, 200
Mva1269I GAATGC 2 cut(s) 84, 403
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 2 cut(s) 77, 170
NciI CCSGG 2 cut(s) 77, 125
NlaIII CATG 2 cut(s) 271, 359
NruI TCGCGA 1 cut(s) 231
NspI RCATGY 1 cut(s) 359
PaeR7I CTCGAG 1 cut(s) 548
PctI GAATGC 2 cut(s) 84, 403
PfeI GAWTC 2 cut(s) 428, 516
PflMI CCANNNNNTGG 1 cut(s) 529
PfoI TCCNGGA 1 cut(s) 198
Psp124BI GAGCTC 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PspPI GGNCC 1 cut(s) 78
RruI TCGCGA 1 cut(s) 231
RsaI GTAC 2 cut(s) 156, 275
RsaNI GTAC 2 cut(s) 155, 274
SacI GAGCTC 1 cut(s) 306
SalI GTCGAC 1 cut(s) 183
SaqAI TTAA 5 cut(s) 345, 419, 567, 623, 630
Sau96I GGNCC 1 cut(s) 78
ScaI AGTACT 1 cut(s) 275
ScrFI CCNGG 3 cut(s) 77, 125, 200
SduI GDGCHC 1 cut(s) 306
SetI ASST 7 cut(s) 109, 156, 306, 393, 415, 528, 614
Sfr274I CTCGAG 1 cut(s) 548
SlaI CTCGAG 1 cut(s) 548
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 4 cut(s) 254, 489, 553, 591
SspI AATATT 1 cut(s) 407
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 3 cut(s) 75, 123, 198
TaaI ACNGT 4 cut(s) 216, 278, 287, 296
TaiI ACGT 1 cut(s) 109
TaqI TCGA 4 cut(s) 184, 549, 582, 608
TasI AATT 4 cut(s) 254, 489, 553, 591
TatI WGTACW 1 cut(s) 273
TfiI GAWTC 2 cut(s) 428, 516
Tru1I TTAA 5 cut(s) 345, 419, 567, 623, 630
Tru9I TTAA 5 cut(s) 345, 419, 567, 623, 630
TscAI CASTG 2 cut(s) 219, 567
TspDTI ATGAA 1 cut(s) 600
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 2 cut(s) 219, 567
Van91I CCANNNNNTGG 1 cut(s) 529
XapI RAATTY 2 cut(s) 254, 553
XceI RCATGY 1 cut(s) 359
XhoI CTCGAG 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 184
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.