Rh4DG440500

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007978
Physical Location & Seq
Reverse (-)
64224359 .. 64227245
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG440500.1

Sequence Viewer

Length: 1683 bp
ATGTCACACCTCTGTGATTTTCAAATCCACATTAATGGTCTGCAAACATTCTTTGTGAATGAGGAGGTTTTATCAACATATGCAGGGAGGTTGAAGAAGATTATCAAGCAAGAAAGGAGAAGATCCCAGATCAAGTACTCGGGGATTGAAATCGAAGACTTCCCTGGAGGCCCTGATGGGTTTGAGTTGATCTCAAGGTTCTGTTACAGCAATGGAAGAATCACAACAACAGTTTCAAATGTGTCCCTCCTCCATTGCTGTGCGGTCTTTCTTGGTATGACTGAGAAAGTCTCAACCGACAATCTCTTGAAACAAACAGAAACCTTTCTTGAGGGACTCTTTGACTGGTCATGGAAAGAAATACTAGTCTGCCTGAAAAGCTGCGAGTCATTCTTGAACTATGCAGATTCTTCTGGTCTTGTAGACAAGCTCATTTGTGCAGTTTTAGCAAAGATTGCTCAGAATTCAGACATAGGCAGCCTCATTGCTACTTCGTCTTCGTCGTCTTCATCTCCAGAAACTACATCTGGGTTCAGAGTGTCTTCCTCATTTAAGAACACCCCTGAGTCACTCAAGCCAAGTTCATCAAGTAGAGCTTGGTGGTTTGATGATGTGTCCATTTTACCCCCAAAGATCATTGAGAAACTCATCCAAAGCTTAGGGGCGTATGGTTCTGAAAACAACAGCTTGATCCTCACAAGGTTTCTCCTCCATTACCTGAAAATTTCAGCTCAAAGAAAGAAAGGCAATACTAGTCACAGATCATCCGAGTTTAGTGCTCTTGCAGACACGGCTATTCATGGGATTATTTTGGTGGGTAAAAATGTCTTTTCCTGCAGAGCCTTGTTTTGGGTTTTGAGGATTGTGTCTGGTTTTGGAATCAGTAGAGAGTACAGACTTGGTTTGGAGAGATTGATTGGTGGGATGCTTGATGAAGCAACATTGGATGACTTGTTAGTATCTGGGCATGATAGGGGTGTTTATGATGTCAATCTTGTTATAAGGTTGATAAGAGTATTTGCTAAAAGTGAGGGGCTTTCTGTGCAAAAGTTGAGGAAAGCTGGTAGGTTGATCGATAAGTACCTAGGTGAAATATCACCTGATCAGAATCTCAAGATATCTAAATTTCTTGGAGTTGCAGAGAGTTTACCAGATTCTGCTAGGGAGTGCTTTGATGGGGCCTACAGAGCCATTGATATTTATCTTGAGTCTCACCCGACTCTTTCATTTGAGGAGAGGTCAAGATTATGCAGATGCCTAAATTATGAAAAGCTGAGCCTCGAAGCGTGTAAAGAGCTTGCAAAGAATCCGAAAATCCCACCAAGAGTTGCAATTCAAGCACTCATTTCTCAGCAATCCAAAGTAACAACAACAGAAGACTTTGTACATGCCAGTCCAAGGAGTACTGCAACTGCAAGTGATCATTTTCATTCCCATTCACATTCCAATTCCCATTCCCAAATTGTTCTGTACAACAAGGGTGGTGTCGGTGTTGATAAAGATAGTTTTCCGGAAGAAAGCAGCTTAGATACGAAGCTAAATTTGCAGAGGATGCAATGGAGGGTGGTGGAGTTGGAGAAACTGTGCAGGGAAATGAAGGGTCAGATGTCAAGGATGGTTAAGAATAATGTCTTGAGTAGTAGTACCCCAACTCATGGTAGAGCTCTTCCTAGACTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

560

Amino Acids

62.5

Weight (kDa)

8.71

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 200 - 430 4.2e-60 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1001
AccB7I CCANNNNNTGG 1 cut(s) 1655
AccI GTMKAC 1 cut(s) 423
AccIII TCCGGA 1 cut(s) 1510
AciI CCGC 1 cut(s) 263
AclWI GGATC 2 cut(s) 117, 685
AcsI RAATTY 4 cut(s) 463, 723, 1124, 1540
AfaI GTAC 7 cut(s) 137, 893, 1082, 1386, 1405, 1472, 1645
AfiI CCNNNNNNNGG 3 cut(s) 849, 1398, 1655
AgsI TTSAA 7 cut(s) 23, 94, 149, 237, 310, 397, 1337
AhlI ACTAGT 2 cut(s) 364, 752
AjnI CCWGG 1 cut(s) 163
AleI CACNNNNGTG 1 cut(s) 12
Alw21I GWGCWC 2 cut(s) 781, 1666
Alw26I GTCTC 2 cut(s) 295, 1215
AlwI GGATC 2 cut(s) 117, 685
AlwNI CAGNNNCTG 1 cut(s) 1157
Ama87I CYCGRG 1 cut(s) 139
Aor13HI TCCGGA 1 cut(s) 1510
AoxI GGCC 2 cut(s) 169, 1179
ApeKI GCWGC 3 cut(s) 381, 477, 1521
ApoI RAATTY 4 cut(s) 463, 723, 1124, 1540
AseI ATTAAT 1 cut(s) 33
Asp700I GAANNNNTTC 1 cut(s) 324
AspA2I CCTAGG 1 cut(s) 1084
AspS9I GGNCC 2 cut(s) 170, 1179
AsuHPI GGTGA 3 cut(s) 1089, 1100, 1205
AvaI CYCGRG 1 cut(s) 139
AvrII CCTAGG 1 cut(s) 1084
BanII GRGCYC 1 cut(s) 1666
BarI GAAGNNNNNNTAC 2 cut(s) 1368, 1400
BbsI GAAGAC 5 cut(s) 162, 489, 498, 534, 1383
Bbv12I GWGCWC 2 cut(s) 781, 1666
BbvI GCAGC 3 cut(s) 368, 489, 1533
BccI CCATC 3 cut(s) 170, 1169, 1609
BceAI ACGGC 1 cut(s) 807
BcgI CGANNNNNNTGC 2 cut(s) 758, 792
BciT130I CCWGG 1 cut(s) 165
BclI TGATCA 2 cut(s) 1102, 1420
BcoDI GTCTC 2 cut(s) 295, 1215
BcuI ACTAGT 2 cut(s) 364, 752
BfaI CTAG 5 cut(s) 365, 753, 1085, 1161, 1671
BfmI CTRYAG 2 cut(s) 835, 1183
BisI GCNGC 3 cut(s) 382, 478, 1522
BlnI CCTAGG 1 cut(s) 1084
BlpI GCTNAGC 1 cut(s) 1274
BlsI GCNGC 3 cut(s) 383, 479, 1523
BmcAI AGTACT 2 cut(s) 137, 1405
Bme1390I CCNGG 1 cut(s) 165
BmeT110I CYCGRG 1 cut(s) 139
BmgT120I GGNCC 2 cut(s) 170, 1179
BmiI GGNNCC 1 cut(s) 1180
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 3 cut(s) 915, 1244, 1542
BpiI GAAGAC 5 cut(s) 162, 489, 498, 534, 1383
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 275, 307
BpmI CTGGAG 2 cut(s) 186, 498
Bpu10I CCTNAGC 1 cut(s) 658
Bpu1102I GCTNAGC 1 cut(s) 1274
BpuEI CTTGAG 6 cut(s) 178, 350, 557, 1097, 1226, 1654
Bsa29I ATCGAT 1 cut(s) 1074
BsaBI GATNNNNATC 4 cut(s) 149, 990, 1107, 1200
BsaJI CCNNGG 3 cut(s) 163, 1084, 1397
BsaWI WCCGGW 1 cut(s) 1510
BsaXI ACNNNNNCTCC 2 cut(s) 1568, 1598
Bsc4I CCNNNNNNNGG 3 cut(s) 849, 1398, 1655
Bse1I ACTGG 2 cut(s) 350, 1392
Bse3DI GCAATG 4 cut(s) 217, 253, 483, 1562
Bse8I GATNNNNATC 4 cut(s) 149, 990, 1107, 1200
BseAI TCCGGA 1 cut(s) 1510
BseBI CCWGG 1 cut(s) 165
BseCI ATCGAT 1 cut(s) 1074
BseDI CCNNGG 3 cut(s) 163, 1084, 1397
BseGI GGATG 6 cut(s) 648, 764, 930, 952, 1557, 1620
BseJI GATNNNNATC 4 cut(s) 149, 990, 1107, 1200
BseLI CCNNNNNNNGG 3 cut(s) 849, 1398, 1655
BseMI GCAATG 4 cut(s) 217, 253, 483, 1562
BseMII CTCAG 5 cut(s) 273, 473, 555, 1265, 1364
BseNI ACTGG 2 cut(s) 350, 1392
BseRI GAGGAG 4 cut(s) 77, 239, 698, 1247
BseXI GCAGC 3 cut(s) 368, 489, 1533
BsgI GTGCAG 2 cut(s) 459, 1606
BshFI GGCC 2 cut(s) 171, 1181
BshVI ATCGAT 1 cut(s) 1074
BsiHKAI GWGCWC 2 cut(s) 781, 1666
BsiHKCI CYCGRG 1 cut(s) 139
BsiSI CCGG 1 cut(s) 1511
BslFI GGGAC 2 cut(s) 229, 348
BslI CCNNNNNNNGG 3 cut(s) 849, 1398, 1655
BsmAI GTCTC 2 cut(s) 295, 1215
BsmFI GGGAC 2 cut(s) 229, 348
BsnI GGCC 2 cut(s) 171, 1181
BsoBI CYCGRG 1 cut(s) 139
Bsp1286I GDGCHC 2 cut(s) 781, 1666
Bsp13I TCCGGA 1 cut(s) 1510
Bsp1407I TGTACA 2 cut(s) 1384, 1470
Bsp143I GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
Bsp1720I GCTNAGC 1 cut(s) 1274
BspACI CCGC 1 cut(s) 263
BspANI GGCC 2 cut(s) 171, 1181
BspCNI CTCAG 5 cut(s) 274, 472, 556, 1266, 1363
BspDI ATCGAT 1 cut(s) 1074
BspEI TCCGGA 1 cut(s) 1510
BspLI GGNNCC 1 cut(s) 1180
BspMAI CTGCAG 1 cut(s) 839
BspPI GGATC 2 cut(s) 117, 685
BspQI GCTCTTC 1 cut(s) 1671
BsrDI GCAATG 4 cut(s) 217, 253, 483, 1562
BsrGI TGTACA 2 cut(s) 1384, 1470
BsrI ACTGG 2 cut(s) 350, 1392
BssECI CCNNGG 3 cut(s) 163, 1084, 1397
BssMI GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
BssT1I CCWWGG 2 cut(s) 1084, 1397
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 232, 1584
Bst6I CTCTTC 1 cut(s) 1671
BstAPI GCANNNNNTGC 2 cut(s) 455, 1552
BstAUI TGTACA 2 cut(s) 1384, 1470
BstC8I GCNNGC 1 cut(s) 1299
BstDEI CTNAG 7 cut(s) 282, 459, 564, 658, 1274, 1350, 1525
BstF5I GGATG 6 cut(s) 648, 764, 930, 952, 1557, 1620
BstKTI GATC 9 cut(s) 125, 132, 192, 636, 693, 764, 1074, 1105, 1423
BstMAI GTCTC 2 cut(s) 295, 1215
BstMBI GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
BstMWI GCNNNNNNNGC 9 cut(s) 378, 446, 455, 791, 1042, 1187, 1337, 1543, 1552
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 1 cut(s) 1391
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 2 cut(s) 835, 1183
BstV1I GCAGC 3 cut(s) 368, 489, 1533
BstV2I GAAGAC 5 cut(s) 162, 489, 498, 534, 1383
BstX2I RGATCY 1 cut(s) 122
BstXI CCANNNNNNTGG 1 cut(s) 35
BstYI RGATCY 1 cut(s) 122
Bsu15I ATCGAT 1 cut(s) 1074
BsuRI GGCC 2 cut(s) 171, 1181
BsuTUI ATCGAT 1 cut(s) 1074
BtsCI GGATG 6 cut(s) 648, 764, 930, 952, 1557, 1620
Cac8I GCNNGC 1 cut(s) 1299
CaiI CAGNNNCTG 1 cut(s) 1157
Cfr13I GGNCC 2 cut(s) 170, 1179
ClaI ATCGAT 1 cut(s) 1074
Csp6I GTAC 7 cut(s) 136, 892, 1081, 1385, 1404, 1471, 1644
CspCI CAANNNNNGTGG 2 cut(s) 1462, 1497
CviAII CATG 5 cut(s) 351, 800, 968, 1388, 1655
CviQI GTAC 7 cut(s) 136, 892, 1081, 1385, 1404, 1471, 1644
DdeI CTNAG 7 cut(s) 282, 459, 564, 658, 1274, 1350, 1525
DpnI GATC 9 cut(s) 124, 131, 191, 635, 692, 763, 1073, 1104, 1422
DpnII GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
Eam1104I CTCTTC 1 cut(s) 1671
EarI CTCTTC 1 cut(s) 1671
Ecl136II GAGCTC 1 cut(s) 1664
Eco130I CCWWGG 2 cut(s) 1084, 1397
Eco24I GRGCYC 1 cut(s) 1666
Eco32I GATATC 1 cut(s) 1119
Eco53kI GAGCTC 1 cut(s) 1664
Eco88I CYCGRG 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 1664
EcoO109I RGGNCCY 2 cut(s) 170, 1179
EcoRI GAATTC 1 cut(s) 463
EcoRII CCWGG 1 cut(s) 163
EcoRV GATATC 1 cut(s) 1119
EcoT14I CCWWGG 2 cut(s) 1084, 1397
EcoT38I GRGCYC 1 cut(s) 1666
ErhI CCWWGG 2 cut(s) 1084, 1397
FaeI CATG 5 cut(s) 354, 803, 971, 1391, 1658
FalI AAGNNNNNCTT 2 cut(s) 580, 612
FaqI GGGAC 2 cut(s) 229, 348
FatI CATG 5 cut(s) 350, 799, 967, 1387, 1654
FauNDI CATATG 1 cut(s) 79
FbaI TGATCA 2 cut(s) 1102, 1420
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 3 cut(s) 382, 478, 1522
FokI GGATG 6 cut(s) 635, 751, 937, 959, 1564, 1627
FriOI GRGCYC 1 cut(s) 1666
Fsp4HI GCNGC 3 cut(s) 382, 478, 1522
FspBI CTAG 5 cut(s) 365, 753, 1085, 1161, 1671
GluI GCNGC 3 cut(s) 382, 478, 1522
GsuI CTGGAG 2 cut(s) 186, 498
HaeIII GGCC 2 cut(s) 171, 1181
HapII CCGG 1 cut(s) 1511
Hin1II CATG 5 cut(s) 354, 803, 971, 1391, 1658
HindIII AAGCTT 1 cut(s) 655
HpaII CCGG 1 cut(s) 1511
HphI GGTGA 3 cut(s) 1089, 1100, 1205
Hpy166II GTNNAC 2 cut(s) 424, 1148
Hpy188I TCNGA 8 cut(s) 462, 469, 536, 676, 769, 1107, 1311, 1605
Hpy188III TCNNGA 9 cut(s) 307, 329, 394, 515, 1114, 1205, 1242, 1511, 1633
Hpy8I GTNNAC 2 cut(s) 424, 1148
Hpy99I CGWCG 1 cut(s) 505
HpyAV CCTTC 1 cut(s) 1591
HpyCH4III ACNGT 2 cut(s) 232, 1584
HpyF10VI GCNNNNNNNGC 9 cut(s) 378, 446, 455, 791, 1042, 1187, 1337, 1543, 1552
HpyF3I CTNAG 7 cut(s) 282, 459, 564, 658, 1274, 1350, 1525
Hsp92II CATG 5 cut(s) 354, 803, 971, 1391, 1658
Kpn2I TCCGGA 1 cut(s) 1510
Ksp22I TGATCA 2 cut(s) 1102, 1420
Kzo9I GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
LguI GCTCTTC 1 cut(s) 1671
Lsp1109I GCAGC 3 cut(s) 368, 489, 1533
LweI GCATC 3 cut(s) 915, 1244, 1542
MaeI CTAG 5 cut(s) 365, 753, 1085, 1161, 1671
MaeIII GTNAC 5 cut(s) 3, 203, 567, 755, 1363
MalI GATC 9 cut(s) 124, 131, 191, 635, 692, 763, 1073, 1104, 1422
MboI GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
MflI RGATCY 1 cut(s) 122
MhlI GDGCHC 2 cut(s) 781, 1666
MluCI AATT 8 cut(s) 463, 723, 1124, 1261, 1332, 1447, 1461, 1540
MlyI GAGTC 5 cut(s) 330, 395, 575, 1213, 1217
MmeI TCCRAC 1 cut(s) 1554
MroI TCCGGA 1 cut(s) 1510
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 3 cut(s) 33, 552, 1620
MslI CAYNNNNRTG 3 cut(s) 12, 33, 258
MspI CCGG 1 cut(s) 1511
MspR9I CCNGG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 165
MwoI GCNNNNNNNGC 9 cut(s) 378, 446, 455, 791, 1042, 1187, 1337, 1543, 1552
NdeI CATATG 1 cut(s) 79
NdeII GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
NlaIII CATG 5 cut(s) 354, 803, 971, 1391, 1658
NlaIV GGNNCC 1 cut(s) 1180
NmuCI GTSAC 3 cut(s) 3, 567, 755
NspI RCATGY 1 cut(s) 1391
OliI CACNNNNGTG 1 cut(s) 12
PciSI GCTCTTC 1 cut(s) 1671
PcsI WCGNNNNNNNCGW 1 cut(s) 500
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 6 cut(s) 219, 407, 879, 1108, 1154, 1306
PflMI CCANNNNNTGG 1 cut(s) 1655
PkrI GCNGC 3 cut(s) 383, 479, 1523
PleI GAGTC 5 cut(s) 330, 394, 574, 1213, 1216
PpsI GAGTC 5 cut(s) 330, 394, 574, 1213, 1216
PshBI ATTAAT 1 cut(s) 33
PsiI TTATAA 1 cut(s) 1001
Psp124BI GAGCTC 1 cut(s) 1666
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 1180
PspPI GGNCC 2 cut(s) 170, 1179
PstI CTGCAG 1 cut(s) 839
PstNI CAGNNNCTG 1 cut(s) 1157
PsuI RGATCY 1 cut(s) 122
RsaI GTAC 7 cut(s) 137, 893, 1082, 1386, 1405, 1472, 1645
RsaNI GTAC 7 cut(s) 136, 892, 1081, 1385, 1404, 1471, 1644
RseI CAYNNNNRTG 3 cut(s) 12, 33, 258
SacI GAGCTC 1 cut(s) 1666
SapI GCTCTTC 1 cut(s) 1671
SaqAI TTAA 3 cut(s) 33, 552, 1620
SatI GCNGC 3 cut(s) 382, 478, 1522
Sau3AI GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
Sau96I GGNCC 2 cut(s) 170, 1179
ScaI AGTACT 2 cut(s) 137, 1405
SchI GAGTC 5 cut(s) 330, 395, 575, 1213, 1217
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 2 cut(s) 781, 1666
SfaNI GCATC 3 cut(s) 915, 1244, 1542
SfcI CTRYAG 2 cut(s) 835, 1183
SmiMI CAYNNNNRTG 3 cut(s) 12, 33, 258
SmlI CTYRAG 6 cut(s) 193, 329, 572, 1112, 1205, 1633
SmoI CTYRAG 6 cut(s) 193, 329, 572, 1112, 1205, 1633
SpeI ACTAGT 2 cut(s) 364, 752
Sse9I AATT 8 cut(s) 463, 723, 1124, 1261, 1332, 1447, 1461, 1540
SsiI CCGC 1 cut(s) 263
SspMI CTAG 5 cut(s) 365, 753, 1085, 1161, 1671
SstI GAGCTC 1 cut(s) 1666
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 2 cut(s) 1084, 1397
TaaI ACNGT 2 cut(s) 232, 1584
TaqI TCGA 3 cut(s) 153, 1074, 1281
TasI AATT 8 cut(s) 463, 723, 1124, 1261, 1332, 1447, 1461, 1540
TatI WGTACW 5 cut(s) 135, 891, 1384, 1403, 1470
TfiI GAWTC 6 cut(s) 219, 407, 879, 1108, 1154, 1306
Tru1I TTAA 3 cut(s) 33, 552, 1620
Tru9I TTAA 3 cut(s) 33, 552, 1620
TseFI GTSAC 3 cut(s) 3, 567, 755
TseI GCWGC 3 cut(s) 381, 477, 1521
Tsp45I GTSAC 3 cut(s) 3, 567, 755
TspDTI ATGAA 8 cut(s) 498, 573, 788, 948, 1215, 1281, 1418, 1610
Van91I CCANNNNNTGG 1 cut(s) 1655
VspI ATTAAT 1 cut(s) 33
XapI RAATTY 4 cut(s) 463, 723, 1124, 1540
XceI RCATGY 1 cut(s) 1391
XmaJI CCTAGG 1 cut(s) 1084
XmiI GTMKAC 1 cut(s) 423
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 5 cut(s) 365, 753, 1085, 1161, 1671
ZrmI AGTACT 2 cut(s) 137, 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.