pycom13g00670

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007978
Physical Location & Seq
Forward (+)
426819 .. 428249
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00670.1

Sequence Viewer

Length: 1326 bp
ATGACCGAGAACCTTGCACCCCGCAATTTGTTGCAGAAAACAAATACTTTTCTCGACGGATTTTTTGAGTGGTCGTTGAAAGACGTACTAGCCTGCCTGAAGAGCTGCCACTCGTTCTTCGACTACGCAGATTCGTCCGGTCTTTTAGACAAACTCATTACTGCACTGCTAACAAAGATCGCTCAGAATTCTGACGTAAGCAACCTCATTGCCTCGTCGCCTTCGTCTTCTCCGGAAACTGCATCTGGGTTCAGACTCTCTTCCAACACGCCAAAATCGATCAGGCCGAGCTCGTCCAGCAGAGCGTGGTGGTTCGACGACGTGGCGGTTCTGCCACCAAATATCATTGCAAAACTCCTCCAGATTTTAGGTGCTTACGGACCCAAAAACAACAGCCTGATCCTCACCAGATTCCTCCTCCACTACTTGAAAATCTCAGCCCAGAGAAAACCCGCGAACTCGAAATGCGAACTCGGGAGGCTCGCAGACACGGCGGTTCACGGCGTGATCTTGGTGGGCAAAAAGGTGTTTTCATGCAGAGCTCTGTTCTGGGTTCTGTGGATTGTGTCTGGATTTGGAATTAGCAAAGAGTACAAAGTTGGGTTGGAGAGGTTGATTGGTGGGATGATCGATGAAGCCACGCTGGATGATTTGCTGGTGTCGGGGCACGACAGGGGAATTTATGATGTTAATCTCGTGATTAGGATGTTAAGAGTGTTTCTTAATGGCGAAGGAGTTTCAGTGCAGAGGTTGAAGAAAGCTGGGAAATTGGTTGATAAGTACTTGGGTGAAATATCACCTGACCAGAATCTCAAGATTTCTAAGTTTCTGGGAGTTGCAGAGAGCTTGCCTGATTGTGCTAGGGACTGCTTTGATGCGGCCTACAGAGCCATTGATGTCTATCTTGAGGCTCATCCAAATCTTTCGCTCGAACAGCGGGCGAGATTATGCAGATGCCTAAATTACGAGAAGCTGAGCTTAGAAGCATGTAAAGAGCTTGCCAAGAATCCTAAAATCCCACCAAGGTTTGCCATTCGAGCACTTATTTCTCAAAACTCCAAAATAACACCAACACAGCCAAAACTGGTTGTATATCAGAGTCCGCGTTCTCATTTCCATTCATGTTCCGATATGGTTTGGTACAAGACCGGCGGTGCGGAAGAAGACGAATCGACGTCGTTAAACTTGCAGAGGATGCAATGGAGGGTGGTGGAGTTGGAGAAACTGTGCAGGCAAATGAAGGGGCAAGTGTCAAGGATGGTAAAACATAATGTCTTGACGACTATCGCTCCAACTCACGGTAGAGCTTTTCCCAGACTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

442

Amino Acids

49.29

Weight (kDa)

9.3

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 103 - 330 3.8e-54 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1178
AccII CGCG 2 cut(s) 455, 1105
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 9 cut(s) 22, 326, 453, 494, 878, 937, 1103, 1152, 1157
AclWI GGATC 1 cut(s) 394
AcsI RAATTY 2 cut(s) 187, 678
AcuI CTGAAG 1 cut(s) 119
AcyI GRCGYC 1 cut(s) 1175
AdeI CACNNNGTG 1 cut(s) 505
AfaI GTAC 4 cut(s) 87, 593, 782, 1142
AfiI CCNNNNNNNGG 1 cut(s) 1298
AgsI TTSAA 3 cut(s) 79, 430, 754
AjiI CACGTC 1 cut(s) 322
AluBI AGCT 9 cut(s) 105, 291, 542, 761, 846, 973, 978, 997, 1307
AluI AGCT 9 cut(s) 105, 291, 542, 761, 846, 973, 978, 997, 1307
Alw21I GWGCWC 3 cut(s) 293, 544, 1042
AlwI GGATC 1 cut(s) 394
Ama87I CYCGRG 1 cut(s) 473
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 2 cut(s) 284, 879
ApeKI GCWGC 1 cut(s) 105
ApoI RAATTY 2 cut(s) 187, 678
ArsI GACNNNNNNTTYG 2 cut(s) 47, 79
AspS9I GGNCC 1 cut(s) 380
AsuHPI GGTGA 3 cut(s) 397, 789, 800
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 1 cut(s) 380
BaeGI GKGCMC 1 cut(s) 669
BanII GRGCYC 2 cut(s) 293, 544
BauI CACGAG 1 cut(s) 695
BbsI GAAGAC 2 cut(s) 219, 1170
Bbv12I GWGCWC 3 cut(s) 293, 544, 1042
BbvI GCAGC 1 cut(s) 92
BccI CCATC 1 cut(s) 1252
BceAI ACGGC 2 cut(s) 507, 517
BcgI CGANNNNNNTGC 1 cut(s) 30
BfaI CTAG 2 cut(s) 89, 861
BfmI CTRYAG 1 cut(s) 883
BisI GCNGC 2 cut(s) 106, 879
BlpI GCTNAGC 1 cut(s) 974
BlsI GCNGC 2 cut(s) 107, 880
BmcAI AGTACT 1 cut(s) 782
Bme18I GGWCC 1 cut(s) 380
BmeT110I CYCGRG 1 cut(s) 473
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 380
BmiI GGNNCC 1 cut(s) 382
BmsI GCATC 4 cut(s) 251, 865, 944, 1185
BpiI GAAGAC 2 cut(s) 219, 1170
BpmI CTGGAG 1 cut(s) 344
Bpu1102I GCTNAGC 1 cut(s) 974
BpuEI CTTGAG 2 cut(s) 797, 926
Bsa29I ATCGAT 2 cut(s) 278, 630
BsaBI GATNNNNATC 2 cut(s) 690, 900
BsaHI GRCGYC 1 cut(s) 1175
BsaJI CCNNGG 1 cut(s) 1022
BsaWI WCCGGW 2 cut(s) 137, 232
BsaXI ACNNNNNCTCC 6 cut(s) 726, 756, 1211, 1241, 1273, 1303
Bsc4I CCNNNNNNNGG 1 cut(s) 1298
Bse118I RCCGGY 1 cut(s) 1148
Bse1I ACTGG 1 cut(s) 1089
Bse3DI GCAATG 3 cut(s) 207, 345, 1205
Bse8I GATNNNNATC 2 cut(s) 690, 900
BseAI TCCGGA 1 cut(s) 232
BseCI ATCGAT 2 cut(s) 278, 630
BseDI CCNNGG 1 cut(s) 1022
BseGI GGATG 6 cut(s) 630, 652, 711, 913, 1200, 1263
BseJI GATNNNNATC 2 cut(s) 690, 900
BseLI CCNNNNNNNGG 1 cut(s) 1298
BseMI GCAATG 3 cut(s) 207, 345, 1205
BseMII CTCAG 3 cut(s) 197, 450, 965
BseNI ACTGG 1 cut(s) 1089
BseRI GAGGAG 2 cut(s) 347, 407
BseSI GKGCMC 1 cut(s) 669
BseXI GCAGC 1 cut(s) 92
BseYI CCCAGC 1 cut(s) 761
BsgI GTGCAG 3 cut(s) 147, 764, 1249
Bsh1236I CGCG 2 cut(s) 455, 1105
BshFI GGCC 2 cut(s) 286, 881
BshVI ATCGAT 2 cut(s) 278, 630
BsiHKAI GWGCWC 3 cut(s) 293, 544, 1042
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 3 cut(s) 138, 233, 1149
BslFI GGGAC 1 cut(s) 878
BslI CCNNNNNNNGG 1 cut(s) 1298
BsmFI GGGAC 1 cut(s) 878
BsnI GGCC 2 cut(s) 286, 881
BsoBI CYCGRG 1 cut(s) 473
Bsp1286I GDGCHC 4 cut(s) 293, 544, 669, 1042
Bsp13I TCCGGA 1 cut(s) 232
Bsp143I GATC 5 cut(s) 177, 279, 399, 507, 627
Bsp1720I GCTNAGC 1 cut(s) 974
BspACI CCGC 9 cut(s) 22, 326, 453, 494, 878, 937, 1103, 1152, 1157
BspANI GGCC 2 cut(s) 286, 881
BspCNI CTCAG 3 cut(s) 196, 449, 966
BspDI ATCGAT 2 cut(s) 278, 630
BspEI TCCGGA 1 cut(s) 232
BspFNI CGCG 2 cut(s) 455, 1105
BspLI GGNNCC 1 cut(s) 382
BspPI GGATC 1 cut(s) 394
BspQI GCTCTTC 1 cut(s) 95
BsrDI GCAATG 3 cut(s) 207, 345, 1205
BsrFI RCCGGY 1 cut(s) 1148
BsrI ACTGG 1 cut(s) 1089
BssAI RCCGGY 1 cut(s) 1148
BssECI CCNNGG 1 cut(s) 1022
BssMI GATC 5 cut(s) 177, 279, 399, 507, 627
BssNI GRCGYC 1 cut(s) 1175
BssSI CACGAG 1 cut(s) 695
BssT1I CCWWGG 1 cut(s) 1022
Bst2BI CACGAG 1 cut(s) 695
Bst4CI ACNGT 2 cut(s) 1227, 1301
Bst6I CTCTTC 2 cut(s) 95, 265
BstACI GRCGYC 1 cut(s) 1175
BstAPI GCANNNNNTGC 1 cut(s) 1195
BstC8I GCNNGC 6 cut(s) 94, 483, 848, 939, 999, 1232
BstDEI CTNAG 5 cut(s) 183, 436, 822, 974, 979
BstF5I GGATG 6 cut(s) 630, 652, 711, 913, 1200, 1263
BstFNI CGCG 2 cut(s) 455, 1105
BstKTI GATC 5 cut(s) 180, 282, 402, 510, 630
BstMBI GATC 5 cut(s) 177, 279, 399, 507, 627
BstMWI GCNNNNNNNGC 7 cut(s) 102, 297, 491, 887, 934, 1037, 1195
BstNSI RCATGY 1 cut(s) 990
BstSFI CTRYAG 1 cut(s) 883
BstSLI GKGCMC 1 cut(s) 669
BstUI CGCG 2 cut(s) 455, 1105
BstV1I GCAGC 1 cut(s) 92
BstV2I GAAGAC 2 cut(s) 219, 1170
Bsu15I ATCGAT 2 cut(s) 278, 630
BsuRI GGCC 2 cut(s) 286, 881
BsuTUI ATCGAT 2 cut(s) 278, 630
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 6 cut(s) 630, 652, 711, 913, 1200, 1263
BtsI GCAGTG 1 cut(s) 164
BtsIMutI CAGTG 2 cut(s) 164, 747
Cac8I GCNNGC 6 cut(s) 94, 483, 848, 939, 999, 1232
Cfr10I RCCGGY 1 cut(s) 1148
Cfr13I GGNCC 1 cut(s) 380
ClaI ATCGAT 2 cut(s) 278, 630
Csp6I GTAC 4 cut(s) 86, 592, 781, 1141
CviAII CATG 3 cut(s) 534, 987, 1122
CviQI GTAC 4 cut(s) 86, 592, 781, 1141
DdeI CTNAG 5 cut(s) 183, 436, 822, 974, 979
DpnI GATC 5 cut(s) 179, 281, 401, 509, 629
DpnII GATC 5 cut(s) 177, 279, 399, 507, 627
DraIII CACNNNGTG 1 cut(s) 505
Eam1104I CTCTTC 2 cut(s) 95, 265
EarI CTCTTC 2 cut(s) 95, 265
Ecl136II GAGCTC 2 cut(s) 291, 542
Eco130I CCWWGG 1 cut(s) 1022
Eco24I GRGCYC 2 cut(s) 293, 544
Eco47I GGWCC 1 cut(s) 380
Eco53kI GAGCTC 2 cut(s) 291, 542
Eco57I CTGAAG 1 cut(s) 119
Eco88I CYCGRG 1 cut(s) 473
EcoICRI GAGCTC 2 cut(s) 291, 542
EcoRI GAATTC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 1022
EcoT38I GRGCYC 2 cut(s) 293, 544
ErhI CCWWGG 1 cut(s) 1022
FaeI CATG 3 cut(s) 537, 990, 1125
FaiI YATR 8 cut(s) 535, 684, 949, 988, 1093, 1123, 1133, 1269
FalI AAGNNNNNCTT 2 cut(s) 962, 994
FaqI GGGAC 1 cut(s) 878
FatI CATG 3 cut(s) 533, 986, 1121
FauI CCCGC 3 cut(s) 29, 460, 930
Fnu4HI GCNGC 2 cut(s) 106, 879
FokI GGATG 6 cut(s) 637, 659, 718, 900, 1207, 1270
FriOI GRGCYC 2 cut(s) 293, 544
Fsp4HI GCNGC 2 cut(s) 106, 879
FspBI CTAG 2 cut(s) 89, 861
GluI GCNGC 2 cut(s) 106, 879
GsaI CCCAGC 1 cut(s) 765
GsuI CTGGAG 1 cut(s) 344
HaeIII GGCC 2 cut(s) 286, 881
HapII CCGG 3 cut(s) 138, 233, 1149
Hin1I GRCGYC 1 cut(s) 1175
Hin1II CATG 3 cut(s) 537, 990, 1125
HinfI GANTC 7 cut(s) 131, 255, 411, 808, 1006, 1099, 1169
HpaII CCGG 3 cut(s) 138, 233, 1149
HphI GGTGA 3 cut(s) 397, 789, 800
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 5 cut(s) 186, 193, 254, 1098, 1129
Hpy188III TCNNGA 9 cut(s) 53, 233, 361, 475, 570, 697, 814, 905, 1276
Hpy8I GTNNAC 1 cut(s) 499
Hpy99I CGWCG 6 cut(s) 59, 220, 320, 323, 1177, 1180
HpyAV CCTTC 3 cut(s) 231, 725, 1234
HpyCH4III ACNGT 2 cut(s) 1227, 1301
HpyCH4IV ACGT 4 cut(s) 84, 195, 321, 1175
HpyF10VI GCNNNNNNNGC 7 cut(s) 102, 297, 491, 887, 934, 1037, 1195
HpyF3I CTNAG 5 cut(s) 183, 436, 822, 974, 979
HpySE526I ACGT 4 cut(s) 84, 195, 321, 1175
Hsp92I GRCGYC 1 cut(s) 1175
Hsp92II CATG 3 cut(s) 537, 990, 1125
Kpn2I TCCGGA 1 cut(s) 232
Kzo9I GATC 5 cut(s) 177, 279, 399, 507, 627
LguI GCTCTTC 1 cut(s) 95
LmnI GCTCC 1 cut(s) 1294
Lsp1109I GCAGC 1 cut(s) 92
LweI GCATC 4 cut(s) 251, 865, 944, 1185
MaeI CTAG 2 cut(s) 89, 861
MaeII ACGT 4 cut(s) 84, 195, 321, 1175
MalI GATC 5 cut(s) 179, 281, 401, 509, 629
MboI GATC 5 cut(s) 177, 279, 399, 507, 627
MboII GAAGA 7 cut(s) 109, 112, 219, 252, 766, 1172, 1175
MhlI GDGCHC 4 cut(s) 293, 544, 669, 1042
MluCI AATT 6 cut(s) 25, 187, 579, 678, 767, 961
MlyI GAGTC 2 cut(s) 249, 1108
MmeI TCCRAC 4 cut(s) 288, 585, 1197, 1316
MroI TCCGGA 1 cut(s) 232
MseI TTAA 4 cut(s) 690, 710, 723, 1181
MspA1I CMGCKG 1 cut(s) 937
MspI CCGG 3 cut(s) 138, 233, 1149
MvnI CGCG 2 cut(s) 455, 1105
MwoI GCNNNNNNNGC 7 cut(s) 102, 297, 491, 887, 934, 1037, 1195
NdeII GATC 5 cut(s) 177, 279, 399, 507, 627
NlaIII CATG 3 cut(s) 537, 990, 1125
NlaIV GGNNCC 1 cut(s) 382
NmeAIII GCCGAG 1 cut(s) 312
NspI RCATGY 1 cut(s) 990
PciSI GCTCTTC 1 cut(s) 95
PcsI WCGNNNNNNNCGW 3 cut(s) 221, 275, 284
PfeI GAWTC 5 cut(s) 131, 411, 808, 1006, 1169
PkrI GCNGC 2 cut(s) 107, 880
PleI GAGTC 2 cut(s) 249, 1107
PpsI GAGTC 2 cut(s) 249, 1107
Psp124BI GAGCTC 2 cut(s) 293, 544
PspFI CCCAGC 1 cut(s) 761
PspN4I GGNNCC 1 cut(s) 382
PspPI GGNCC 1 cut(s) 380
RsaI GTAC 4 cut(s) 87, 593, 782, 1142
RsaNI GTAC 4 cut(s) 86, 592, 781, 1141
SacI GAGCTC 2 cut(s) 293, 544
SapI GCTCTTC 1 cut(s) 95
SaqAI TTAA 4 cut(s) 690, 710, 723, 1181
SatI GCNGC 2 cut(s) 106, 879
Sau3AI GATC 5 cut(s) 177, 279, 399, 507, 627
Sau96I GGNCC 1 cut(s) 380
ScaI AGTACT 1 cut(s) 782
SchI GAGTC 2 cut(s) 249, 1108
SduI GDGCHC 4 cut(s) 293, 544, 669, 1042
SfaNI GCATC 4 cut(s) 251, 865, 944, 1185
SfcI CTRYAG 1 cut(s) 883
SinI GGWCC 1 cut(s) 380
SmlI CTYRAG 2 cut(s) 812, 905
SmoI CTYRAG 2 cut(s) 812, 905
Sse9I AATT 6 cut(s) 25, 187, 579, 678, 767, 961
SsiI CCGC 9 cut(s) 22, 326, 453, 494, 878, 937, 1103, 1152, 1157
SspMI CTAG 2 cut(s) 89, 861
SstI GAGCTC 2 cut(s) 293, 544
StyI CCWWGG 1 cut(s) 1022
TaaI ACNGT 2 cut(s) 1227, 1301
TaiI ACGT 4 cut(s) 87, 198, 324, 1178
TaqI TCGA 9 cut(s) 54, 120, 278, 315, 461, 630, 930, 1036, 1172
TaqII GACCGA 1 cut(s) 20
TasI AATT 6 cut(s) 25, 187, 579, 678, 767, 961
TatI WGTACW 2 cut(s) 591, 780
TauI GCSGC 1 cut(s) 881
TfiI GAWTC 5 cut(s) 131, 411, 808, 1006, 1169
Tru1I TTAA 4 cut(s) 690, 710, 723, 1181
Tru9I TTAA 4 cut(s) 690, 710, 723, 1181
TscAI CASTG 2 cut(s) 171, 747
TseI GCWGC 1 cut(s) 105
TspDTI ATGAA 4 cut(s) 522, 648, 1110, 1253
TspGWI ACGGA 2 cut(s) 72, 393
TspRI CASTG 2 cut(s) 171, 747
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 2 cut(s) 187, 678
XceI RCATGY 1 cut(s) 990
XspI CTAG 2 cut(s) 89, 861
ZraI GACGTC 1 cut(s) 1176
ZrmI AGTACT 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.