Rh4CG459100

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0007978
Physical Location & Seq
Reverse (-)
69045330 .. 69048005
2676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG459100.1

Sequence Viewer

Length: 1665 bp
ATGTCACACCTCTGTGATTTTCAAATCCACATTAATGGTCTGCAAACATTCTTTGTGAATGAGGAGGTTTTATCAACATATGCAGGGAGGTTGAAGAAGATTATCAAGCAAGAAAGGAGAAGATCCCAGATCAAGTACTCGGGGATTGAAATCGAAGACTTCCCTGGAGGCCCTGATGGGTTTGAGTTGATCTCAAGGTTCTGTTACAGCAATGGAAGAATCACAACAACAGTTTCAAATGTGTCCCTCCTCCATTGCTGTGCAGTCTTTCTTGGTATGACTGAGAAATTCTCAACCAACAATCTCTTGAAACAAACAGAAACCTTTCTTGAGGGACTCTTTGACTGGTCATGGAAAGAAATACTAGTCTGCCTGAAAAGCTGTGAGTCATTCTTGAACTATGCAGATTCTTCTGGTCTTGTAGACAAGCTCATTTGTGCAGTTTTAGCAAAGATTGCTCAGAATTCAGACATAGGCAGCCTCATTGCTACTTCGTCTTCGTCGTCTTCATCTCCAGAAACTACATCTGGGTTCAGAGTGTCTTCCTCATTTAAGAACACCCCTGAGTCACTCAAGCCAAGTTCATCAAGTAGAGCTTGGTGGTTTGATGATGTGTCCATTTTACCCCCAAAGATCATTGAGAAACTCATCCAAAGTTTAGGGGCGTATGGTTCTGAAAACAACAACTTGATCCTCACAAGGTTTCTCCTCCATTACCTGAAAATTTCAGCTCAAAGAAAGAAAGGCAATACTAGTCACAGATCATCTGAGTTTAGTGGTCTTGCAGACACAGCTATTCATGGGGTTATTTTGGTGGGTAAAAATGTCTTTTCCTGCAGAGCCTTGTTTTGGGTTTTGAGGATTGTGTCTGGTTTTGGAATCAGTAGAGAGTACAGACTTGGTTTGGAGAGATTGATTGGTGGGATGCTTGATGAAGCAACATTGGATGACTTGTTAGTATCTGGGCATGATAGGGGTGTTTATGATGTCAATCTTGTTATAAGGTTGATAAGAGTATTTGCTAAAAGTGAGGGGGTTTCTGTGCAAAAGTTGAGGAAAGCTGGTAGGTTGATCGATAAGTACCTAGGTGAGATATCACCTGATCAGAATCTCAAGATTTCTAAATTTCTTGGAGTTGCAGAGAGTTTACCAGATTCTGCTAGGGAGTGCTTTGATGGGGCCTACAGAGCCATTGATATTTATCTTGAGTCTCACCCGACTCTTTCATTTGAGGAGAGGTCGAGATTATGCAGATGCCTAAATTATGAAAAGCTGAGCCTCGAAGCGTGTAAAGAGCTTGCAAAGAATCCGAAAATCCCACCAAGAGTTGCAATTCAAGCACTCATTTCTCAGCAATCCAAAGTAACAACAACAGAAGACTTTGTACATGCCAGTCCAAGGAGTACTGCAACTGCAAGTGATCATTTTCATTCCCATTCCCAAATTGTTCTGTACAACAAGGGTGGTGTCGGTGTTGATAAAGATAGTTTTCCGGAAGAAAGCAGCTTAGATACGAAGCTAAATTTGCAGAGGATGCAATGGAGGGTGGTGGAGTTGGAGAAACTGTGCAGGGAAATGAAGGGTCAGATGTCAAGGATGGTTAAGAATAATGTCTTCAGTAGTAGTACCCCAACTCATGGTAGAGCTCTTCCTAGACTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

554

Amino Acids

61.92

Weight (kDa)

8.78

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 200 - 430 8.9e-60 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1001
AccB7I CCANNNNNTGG 1 cut(s) 1637
AccI GTMKAC 1 cut(s) 423
AccIII TCCGGA 1 cut(s) 1492
AclWI GGATC 2 cut(s) 117, 685
AcsI RAATTY 5 cut(s) 287, 463, 723, 1124, 1522
AcuI CTGAAG 1 cut(s) 1600
AfaI GTAC 7 cut(s) 137, 893, 1082, 1386, 1405, 1454, 1627
AfiI CCNNNNNNNGG 3 cut(s) 849, 1398, 1637
AgsI TTSAA 7 cut(s) 23, 94, 149, 237, 310, 397, 1337
AhlI ACTAGT 2 cut(s) 364, 752
AjnI CCWGG 1 cut(s) 163
AleI CACNNNNGTG 1 cut(s) 12
Alw21I GWGCWC 1 cut(s) 1648
Alw26I GTCTC 1 cut(s) 1215
AlwI GGATC 2 cut(s) 117, 685
AlwNI CAGNNNCTG 1 cut(s) 1157
Ama87I CYCGRG 1 cut(s) 139
Aor13HI TCCGGA 1 cut(s) 1492
AoxI GGCC 2 cut(s) 169, 1179
ApeKI GCWGC 2 cut(s) 477, 1503
ApoI RAATTY 5 cut(s) 287, 463, 723, 1124, 1522
AseI ATTAAT 1 cut(s) 33
Asp700I GAANNNNTTC 1 cut(s) 324
AspA2I CCTAGG 1 cut(s) 1084
AspS9I GGNCC 2 cut(s) 170, 1179
AsuHPI GGTGA 3 cut(s) 1089, 1100, 1205
AvaI CYCGRG 1 cut(s) 139
AvrII CCTAGG 1 cut(s) 1084
BanII GRGCYC 1 cut(s) 1648
BarI GAAGNNNNNNTAC 2 cut(s) 1368, 1400
BbsI GAAGAC 6 cut(s) 162, 489, 498, 534, 1383, 1606
Bbv12I GWGCWC 1 cut(s) 1648
BbvI GCAGC 2 cut(s) 489, 1515
BccI CCATC 3 cut(s) 170, 1169, 1591
BciT130I CCWGG 1 cut(s) 165
BclI TGATCA 2 cut(s) 1102, 1420
BcoDI GTCTC 1 cut(s) 1215
BcuI ACTAGT 2 cut(s) 364, 752
BfaI CTAG 5 cut(s) 365, 753, 1085, 1161, 1653
BfmI CTRYAG 2 cut(s) 835, 1183
BisI GCNGC 2 cut(s) 478, 1504
BlnI CCTAGG 1 cut(s) 1084
BlpI GCTNAGC 1 cut(s) 1274
BlsI GCNGC 2 cut(s) 479, 1505
BmcAI AGTACT 2 cut(s) 137, 1405
Bme1390I CCNGG 1 cut(s) 165
BmeT110I CYCGRG 1 cut(s) 139
BmgT120I GGNCC 2 cut(s) 170, 1179
BmiI GGNNCC 1 cut(s) 1180
BmrFI CCNGG 1 cut(s) 165
BmsI GCATC 3 cut(s) 915, 1244, 1524
BpiI GAAGAC 6 cut(s) 162, 489, 498, 534, 1383, 1606
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 275, 307
BpmI CTGGAG 2 cut(s) 186, 498
Bpu1102I GCTNAGC 1 cut(s) 1274
BpuEI CTTGAG 5 cut(s) 178, 350, 557, 1097, 1226
Bsa29I ATCGAT 1 cut(s) 1074
BsaBI GATNNNNATC 4 cut(s) 149, 990, 1107, 1200
BsaJI CCNNGG 3 cut(s) 163, 1084, 1397
BsaWI WCCGGW 1 cut(s) 1492
BsaXI ACNNNNNCTCC 2 cut(s) 1550, 1580
Bsc4I CCNNNNNNNGG 3 cut(s) 849, 1398, 1637
Bse1I ACTGG 2 cut(s) 350, 1392
Bse3DI GCAATG 4 cut(s) 217, 253, 483, 1544
Bse8I GATNNNNATC 4 cut(s) 149, 990, 1107, 1200
BseAI TCCGGA 1 cut(s) 1492
BseBI CCWGG 1 cut(s) 165
BseCI ATCGAT 1 cut(s) 1074
BseDI CCNNGG 3 cut(s) 163, 1084, 1397
BseGI GGATG 5 cut(s) 648, 930, 952, 1539, 1602
BseJI GATNNNNATC 4 cut(s) 149, 990, 1107, 1200
BseLI CCNNNNNNNGG 3 cut(s) 849, 1398, 1637
BseMI GCAATG 4 cut(s) 217, 253, 483, 1544
BseMII CTCAG 6 cut(s) 273, 473, 555, 759, 1265, 1364
BseNI ACTGG 2 cut(s) 350, 1392
BseRI GAGGAG 4 cut(s) 77, 239, 698, 1247
BseXI GCAGC 2 cut(s) 489, 1515
BsgI GTGCAG 3 cut(s) 282, 459, 1588
BshFI GGCC 2 cut(s) 171, 1181
BshVI ATCGAT 1 cut(s) 1074
BsiHKAI GWGCWC 1 cut(s) 1648
BsiHKCI CYCGRG 1 cut(s) 139
BsiSI CCGG 1 cut(s) 1493
BslFI GGGAC 2 cut(s) 229, 348
BslI CCNNNNNNNGG 3 cut(s) 849, 1398, 1637
BsmAI GTCTC 1 cut(s) 1215
BsmFI GGGAC 2 cut(s) 229, 348
BsnI GGCC 2 cut(s) 171, 1181
BsoBI CYCGRG 1 cut(s) 139
Bsp1286I GDGCHC 1 cut(s) 1648
Bsp13I TCCGGA 1 cut(s) 1492
Bsp1407I TGTACA 2 cut(s) 1384, 1452
Bsp143I GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
Bsp1720I GCTNAGC 1 cut(s) 1274
BspANI GGCC 2 cut(s) 171, 1181
BspCNI CTCAG 6 cut(s) 274, 472, 556, 760, 1266, 1363
BspDI ATCGAT 1 cut(s) 1074
BspEI TCCGGA 1 cut(s) 1492
BspLI GGNNCC 1 cut(s) 1180
BspMAI CTGCAG 1 cut(s) 839
BspPI GGATC 2 cut(s) 117, 685
BspQI GCTCTTC 1 cut(s) 1653
BsrDI GCAATG 4 cut(s) 217, 253, 483, 1544
BsrGI TGTACA 2 cut(s) 1384, 1452
BsrI ACTGG 2 cut(s) 350, 1392
BssECI CCNNGG 3 cut(s) 163, 1084, 1397
BssMI GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
BssT1I CCWWGG 2 cut(s) 1084, 1397
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 2 cut(s) 232, 1566
Bst6I CTCTTC 1 cut(s) 1653
BstAPI GCANNNNNTGC 2 cut(s) 455, 1534
BstAUI TGTACA 2 cut(s) 1384, 1452
BstC8I GCNNGC 1 cut(s) 1299
BstDEI CTNAG 7 cut(s) 282, 459, 564, 768, 1274, 1350, 1507
BstF5I GGATG 5 cut(s) 648, 930, 952, 1539, 1602
BstKTI GATC 9 cut(s) 125, 132, 192, 636, 693, 764, 1074, 1105, 1423
BstMAI GTCTC 1 cut(s) 1215
BstMBI GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
BstMWI GCNNNNNNNGC 8 cut(s) 378, 446, 455, 791, 1187, 1337, 1525, 1534
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 1 cut(s) 1391
BstSCI CCNGG 1 cut(s) 163
BstSFI CTRYAG 2 cut(s) 835, 1183
BstV1I GCAGC 2 cut(s) 489, 1515
BstV2I GAAGAC 6 cut(s) 162, 489, 498, 534, 1383, 1606
BstX2I RGATCY 1 cut(s) 122
BstXI CCANNNNNNTGG 1 cut(s) 35
BstYI RGATCY 1 cut(s) 122
Bsu15I ATCGAT 1 cut(s) 1074
BsuRI GGCC 2 cut(s) 171, 1181
BsuTUI ATCGAT 1 cut(s) 1074
BtsCI GGATG 5 cut(s) 648, 930, 952, 1539, 1602
Cac8I GCNNGC 1 cut(s) 1299
CaiI CAGNNNCTG 1 cut(s) 1157
Cfr13I GGNCC 2 cut(s) 170, 1179
ClaI ATCGAT 1 cut(s) 1074
Csp6I GTAC 7 cut(s) 136, 892, 1081, 1385, 1404, 1453, 1626
CspCI CAANNNNNGTGG 2 cut(s) 1444, 1479
CviAII CATG 5 cut(s) 351, 800, 968, 1388, 1637
CviQI GTAC 7 cut(s) 136, 892, 1081, 1385, 1404, 1453, 1626
DdeI CTNAG 7 cut(s) 282, 459, 564, 768, 1274, 1350, 1507
DpnI GATC 9 cut(s) 124, 131, 191, 635, 692, 763, 1073, 1104, 1422
DpnII GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
Eam1104I CTCTTC 1 cut(s) 1653
EarI CTCTTC 1 cut(s) 1653
Ecl136II GAGCTC 1 cut(s) 1646
Eco130I CCWWGG 2 cut(s) 1084, 1397
Eco24I GRGCYC 1 cut(s) 1648
Eco32I GATATC 1 cut(s) 1095
Eco53kI GAGCTC 1 cut(s) 1646
Eco57I CTGAAG 1 cut(s) 1600
Eco88I CYCGRG 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 1646
EcoO109I RGGNCCY 2 cut(s) 170, 1179
EcoRI GAATTC 1 cut(s) 463
EcoRII CCWGG 1 cut(s) 163
EcoRV GATATC 1 cut(s) 1095
EcoT14I CCWWGG 2 cut(s) 1084, 1397
EcoT38I GRGCYC 1 cut(s) 1648
ErhI CCWWGG 2 cut(s) 1084, 1397
FaeI CATG 5 cut(s) 354, 803, 971, 1391, 1640
FalI AAGNNNNNCTT 2 cut(s) 580, 612
FaqI GGGAC 2 cut(s) 229, 348
FatI CATG 5 cut(s) 350, 799, 967, 1387, 1636
FauNDI CATATG 1 cut(s) 79
FbaI TGATCA 2 cut(s) 1102, 1420
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 2 cut(s) 478, 1504
FokI GGATG 5 cut(s) 635, 937, 959, 1546, 1609
FriOI GRGCYC 1 cut(s) 1648
Fsp4HI GCNGC 2 cut(s) 478, 1504
FspBI CTAG 5 cut(s) 365, 753, 1085, 1161, 1653
GluI GCNGC 2 cut(s) 478, 1504
GsuI CTGGAG 2 cut(s) 186, 498
HaeIII GGCC 2 cut(s) 171, 1181
HapII CCGG 1 cut(s) 1493
Hin1II CATG 5 cut(s) 354, 803, 971, 1391, 1640
HpaII CCGG 1 cut(s) 1493
HphI GGTGA 3 cut(s) 1089, 1100, 1205
Hpy166II GTNNAC 2 cut(s) 424, 1148
Hpy188I TCNGA 8 cut(s) 462, 469, 536, 676, 769, 1107, 1311, 1587
Hpy188III TCNNGA 8 cut(s) 307, 329, 394, 515, 1114, 1205, 1242, 1493
Hpy8I GTNNAC 2 cut(s) 424, 1148
Hpy99I CGWCG 1 cut(s) 505
HpyAV CCTTC 1 cut(s) 1573
HpyCH4III ACNGT 2 cut(s) 232, 1566
HpyF10VI GCNNNNNNNGC 8 cut(s) 378, 446, 455, 791, 1187, 1337, 1525, 1534
HpyF3I CTNAG 7 cut(s) 282, 459, 564, 768, 1274, 1350, 1507
Hsp92II CATG 5 cut(s) 354, 803, 971, 1391, 1640
Kpn2I TCCGGA 1 cut(s) 1492
Ksp22I TGATCA 2 cut(s) 1102, 1420
Kzo9I GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
LguI GCTCTTC 1 cut(s) 1653
Lsp1109I GCAGC 2 cut(s) 489, 1515
LweI GCATC 3 cut(s) 915, 1244, 1524
MaeI CTAG 5 cut(s) 365, 753, 1085, 1161, 1653
MaeIII GTNAC 5 cut(s) 3, 203, 567, 755, 1363
MalI GATC 9 cut(s) 124, 131, 191, 635, 692, 763, 1073, 1104, 1422
MboI GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
MflI RGATCY 1 cut(s) 122
MhlI GDGCHC 1 cut(s) 1648
MluCI AATT 8 cut(s) 287, 463, 723, 1124, 1261, 1332, 1443, 1522
MlyI GAGTC 5 cut(s) 330, 395, 575, 1213, 1217
MmeI TCCRAC 1 cut(s) 1536
MroI TCCGGA 1 cut(s) 1492
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 3 cut(s) 33, 552, 1602
MslI CAYNNNNRTG 3 cut(s) 12, 33, 258
MspI CCGG 1 cut(s) 1493
MspR9I CCNGG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 165
MwoI GCNNNNNNNGC 8 cut(s) 378, 446, 455, 791, 1187, 1337, 1525, 1534
NdeI CATATG 1 cut(s) 79
NdeII GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
NlaIII CATG 5 cut(s) 354, 803, 971, 1391, 1640
NlaIV GGNNCC 1 cut(s) 1180
NmuCI GTSAC 3 cut(s) 3, 567, 755
NspI RCATGY 1 cut(s) 1391
OliI CACNNNNGTG 1 cut(s) 12
PciSI GCTCTTC 1 cut(s) 1653
PcsI WCGNNNNNNNCGW 1 cut(s) 500
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 6 cut(s) 219, 407, 879, 1108, 1154, 1306
PflMI CCANNNNNTGG 1 cut(s) 1637
PkrI GCNGC 2 cut(s) 479, 1505
PleI GAGTC 5 cut(s) 330, 394, 574, 1213, 1216
PpsI GAGTC 5 cut(s) 330, 394, 574, 1213, 1216
PshBI ATTAAT 1 cut(s) 33
PsiI TTATAA 1 cut(s) 1001
Psp124BI GAGCTC 1 cut(s) 1648
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 1180
PspPI GGNCC 2 cut(s) 170, 1179
PstI CTGCAG 1 cut(s) 839
PstNI CAGNNNCTG 1 cut(s) 1157
PsuI RGATCY 1 cut(s) 122
RsaI GTAC 7 cut(s) 137, 893, 1082, 1386, 1405, 1454, 1627
RsaNI GTAC 7 cut(s) 136, 892, 1081, 1385, 1404, 1453, 1626
RseI CAYNNNNRTG 3 cut(s) 12, 33, 258
SacI GAGCTC 1 cut(s) 1648
SapI GCTCTTC 1 cut(s) 1653
SaqAI TTAA 3 cut(s) 33, 552, 1602
SatI GCNGC 2 cut(s) 478, 1504
Sau3AI GATC 9 cut(s) 122, 129, 189, 633, 690, 761, 1071, 1102, 1420
Sau96I GGNCC 2 cut(s) 170, 1179
ScaI AGTACT 2 cut(s) 137, 1405
SchI GAGTC 5 cut(s) 330, 395, 575, 1213, 1217
ScrFI CCNGG 1 cut(s) 165
SduI GDGCHC 1 cut(s) 1648
SfaNI GCATC 3 cut(s) 915, 1244, 1524
SfcI CTRYAG 2 cut(s) 835, 1183
SmiMI CAYNNNNRTG 3 cut(s) 12, 33, 258
SmlI CTYRAG 5 cut(s) 193, 329, 572, 1112, 1205
SmoI CTYRAG 5 cut(s) 193, 329, 572, 1112, 1205
SpeI ACTAGT 2 cut(s) 364, 752
Sse9I AATT 8 cut(s) 287, 463, 723, 1124, 1261, 1332, 1443, 1522
SspMI CTAG 5 cut(s) 365, 753, 1085, 1161, 1653
SstI GAGCTC 1 cut(s) 1648
StyD4I CCNGG 1 cut(s) 163
StyI CCWWGG 2 cut(s) 1084, 1397
TaaI ACNGT 2 cut(s) 232, 1566
TaqI TCGA 4 cut(s) 153, 1074, 1241, 1281
TasI AATT 8 cut(s) 287, 463, 723, 1124, 1261, 1332, 1443, 1522
TatI WGTACW 5 cut(s) 135, 891, 1384, 1403, 1452
TfiI GAWTC 6 cut(s) 219, 407, 879, 1108, 1154, 1306
Tru1I TTAA 3 cut(s) 33, 552, 1602
Tru9I TTAA 3 cut(s) 33, 552, 1602
TseFI GTSAC 3 cut(s) 3, 567, 755
TseI GCWGC 2 cut(s) 477, 1503
Tsp45I GTSAC 3 cut(s) 3, 567, 755
TspDTI ATGAA 8 cut(s) 498, 573, 788, 948, 1215, 1281, 1418, 1592
Van91I CCANNNNNTGG 1 cut(s) 1637
VspI ATTAAT 1 cut(s) 33
XapI RAATTY 5 cut(s) 287, 463, 723, 1124, 1522
XceI RCATGY 1 cut(s) 1391
XmaJI CCTAGG 1 cut(s) 1084
XmiI GTMKAC 1 cut(s) 423
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 5 cut(s) 365, 753, 1085, 1161, 1653
ZrmI AGTACT 2 cut(s) 137, 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.