AT3G29090

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004453
Physical Location & Seq
Forward (+)
11073646 .. 11075478
1833 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G29090.1

Sequence Viewer

Length: 954 bp
ATGGCAACGACTCGAATGGTTAGGGTTTCGCAAGATGGTTCGGGAGATTACTGCTCTGTTCAAGACGCAATTGATTCGGTGCCTTTAGGTAACACTTGCAGAACTGTGATTCGTCTCTCACCTGGGATTTATCGGCAACCTGTGTATGTACCCAAGAGGAAGAATTTCATTACCTTCGCCGGAATCTCACCGGAGATCACGGTTTTGACTTGGAACAATACAGCTTCCAAGATTGAGCATCACCAGGCGTCTAGAGTCATTGGGACTGGTACGTTTGGTTGTGGTAGTGTTATTGTTGAAGGTGAAGATTTTATTGCAGAGAACATTACTTTTGAGAACTCTGCTCCTGAGGGATCAGGGCAAGCTGTGGCGATACGAGTCACTGCAGACCGTTGCGCCTTTTATAATTGTCGATTTCTCGGTTGGCAGGATACATTGTATTTACATCATGGGAAACAATATTTGAAAGACTGTTACATCGAGGGAAGTGTGGATTTCATATTTGGAAACAGTACCGCTCTCTTGGAACACTGCCATATCCACTGCAAATCTCAGGGGTTTATAACAGCACAAAGCCGAAAATCGTCTCAGGAATCTACTGGTTATGTATTCTTAAGATGTGTGATCACGGGTAATGGTCAGAGCGGGTATATGTATCTCGGAAGGCCATGGGGACCATTTGGGAGGGTAGTCCTTGCTTACACTTACATGGACGCGTGTATCAGAAATGTGGGTTGGCATAACTGGGGAAACGCAGAGAACGAGAGAAGTGCTTGCTTTTATGAGTATAGGTGCTTTGGTCCGGGTAGCTGTTCGTCTGAACGTGTTCCGTGGTCGAGAGAACTGATGGATGACGAAGCTGGACACTTTGTGCATCATAGTTTTGTAGATCCTGAACAGGACCGGCCTTGGTTGTGTCTAAGAATGGGTGTGAAAACACCATATTCGGCTTAG

Protein Analysis

317

Amino Acids

35.52

Weight (kDa)

6.59

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 7 - 297 5.3e-71 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 405, 563
AccB1I GGYRCC 1 cut(s) 79
AccBSI CCGCTC 2 cut(s) 518, 645
AccII CGCG 1 cut(s) 716
AciI CCGC 2 cut(s) 516, 645
AclWI GGATC 2 cut(s) 361, 884
AcsI RAATTY 1 cut(s) 163
AcyI GRCGYC 1 cut(s) 248
AdeI CACNNNGTG 1 cut(s) 871
AfaI GTAC 3 cut(s) 150, 271, 514
AflII CTTAAG 1 cut(s) 613
AflIII ACRYGT 2 cut(s) 714, 823
AgsI TTSAA 3 cut(s) 62, 299, 466
AjnI CCWGG 2 cut(s) 121, 243
AjuI GAANNNNNNNTTGG 2 cut(s) 718, 750
AluBI AGCT 4 cut(s) 224, 365, 810, 860
AluI AGCT 4 cut(s) 224, 365, 810, 860
Alw26I GTCTC 2 cut(s) 119, 591
AlwI GGATC 2 cut(s) 361, 884
AoxI GGCC 2 cut(s) 665, 905
ApoI RAATTY 1 cut(s) 163
Asp700I GAANNNNTTC 2 cut(s) 164, 825
AspLEI GCGC 1 cut(s) 398
AspS9I GGNCC 3 cut(s) 674, 800, 901
AsuC2I CCSGG 1 cut(s) 804
AsuHPI GGTGA 4 cut(s) 111, 180, 233, 314
AvaII GGWCC 3 cut(s) 674, 800, 901
AxyI CCTNAGG 1 cut(s) 348
BaeI ACNNNNGTAYC 2 cut(s) 703, 736
BanI GGYRCC 1 cut(s) 79
BccI CCATC 2 cut(s) 29, 841
BcgI CGANNNNNNTGC 4 cut(s) 57, 91, 752, 786
BciT130I CCWGG 2 cut(s) 123, 245
BciVI GTATCC 1 cut(s) 424
BclI TGATCA 1 cut(s) 624
BcnI CCSGG 1 cut(s) 804
BcoDI GTCTC 2 cut(s) 119, 591
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 1 cut(s) 384
BfrI CTTAAG 1 cut(s) 613
BfuI GTATCC 1 cut(s) 424
Bme1390I CCNGG 3 cut(s) 123, 245, 804
Bme18I GGWCC 3 cut(s) 674, 800, 901
BmgT120I GGNCC 3 cut(s) 674, 800, 901
BmiI GGNNCC 2 cut(s) 81, 675
BmrFI CCNGG 3 cut(s) 123, 245, 804
BmrI ACTGGG 1 cut(s) 754
BmsI GCATC 2 cut(s) 247, 883
BmuI ACTGGG 1 cut(s) 754
BpuMI CCSGG 1 cut(s) 804
BsaHI GRCGYC 1 cut(s) 248
BsaJI CCNNGG 4 cut(s) 122, 668, 830, 908
BsaWI WCCGGW 1 cut(s) 190
Bse118I RCCGGY 1 cut(s) 903
Bse1I ACTGG 3 cut(s) 271, 604, 749
Bse21I CCTNAGG 1 cut(s) 348
BseBI CCWGG 2 cut(s) 123, 245
BseDI CCNNGG 4 cut(s) 122, 668, 830, 908
BseGI GGATG 1 cut(s) 856
BseMII CTCAG 3 cut(s) 339, 566, 602
BseNI ACTGG 3 cut(s) 271, 604, 749
Bsh1236I CGCG 1 cut(s) 716
BshFI GGCC 2 cut(s) 667, 907
BshNI GGYRCC 1 cut(s) 79
BsiSI CCGG 4 cut(s) 180, 191, 803, 904
BslFI GGGAC 2 cut(s) 277, 687
BsmAI GTCTC 2 cut(s) 119, 591
BsmBI CGTCTC 2 cut(s) 119, 591
BsmFI GGGAC 2 cut(s) 277, 687
BsnI GGCC 2 cut(s) 667, 907
Bsp143I GATC 4 cut(s) 195, 353, 624, 889
Bsp19I CCATGG 1 cut(s) 668
BspACI CCGC 2 cut(s) 516, 645
BspANI GGCC 2 cut(s) 667, 907
BspCNI CTCAG 3 cut(s) 340, 565, 601
BspFNI CGCG 1 cut(s) 716
BspLI GGNNCC 2 cut(s) 81, 675
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 2 cut(s) 361, 884
BspT107I GGYRCC 1 cut(s) 79
BspTI CTTAAG 1 cut(s) 613
BsrBI CCGCTC 2 cut(s) 518, 645
BsrFI RCCGGY 1 cut(s) 903
BsrI ACTGG 3 cut(s) 271, 604, 749
BssAI RCCGGY 1 cut(s) 903
BssECI CCNNGG 4 cut(s) 122, 668, 830, 908
BssMI GATC 4 cut(s) 195, 353, 624, 889
BssNI GRCGYC 1 cut(s) 248
BssT1I CCWWGG 2 cut(s) 668, 908
Bst2UI CCWGG 2 cut(s) 123, 245
Bst4CI ACNGT 5 cut(s) 106, 202, 392, 473, 512
BstACI GRCGYC 1 cut(s) 248
BstAFI CTTAAG 1 cut(s) 613
BstC8I GCNNGC 2 cut(s) 363, 775
BstDEI CTNAG 5 cut(s) 348, 552, 588, 920, 951
BstDSI CCRYGG 2 cut(s) 668, 830
BstF5I GGATG 1 cut(s) 856
BstFNI CGCG 1 cut(s) 716
BstHHI GCGC 1 cut(s) 398
BstKTI GATC 4 cut(s) 198, 356, 627, 892
BstMAI GTCTC 2 cut(s) 119, 591
BstMBI GATC 4 cut(s) 195, 353, 624, 889
BstNI CCWGG 2 cut(s) 123, 245
BstSCI CCNGG 3 cut(s) 121, 243, 802
BstSFI CTRYAG 1 cut(s) 384
BstUI CGCG 1 cut(s) 716
BstX2I RGATCY 1 cut(s) 889
BstYI RGATCY 1 cut(s) 889
Bsu36I CCTNAGG 1 cut(s) 348
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 2 cut(s) 667, 907
BtgI CCRYGG 2 cut(s) 668, 830
BtsCI GGATG 1 cut(s) 856
BtsI GCAGTG 3 cut(s) 381, 529, 541
BtsIMutI CAGTG 3 cut(s) 381, 529, 541
Cac8I GCNNGC 2 cut(s) 363, 775
CfoI GCGC 1 cut(s) 398
Cfr10I RCCGGY 1 cut(s) 903
Cfr13I GGNCC 3 cut(s) 674, 800, 901
CseI GACGC 3 cut(s) 74, 237, 722
Csp6I GTAC 3 cut(s) 149, 270, 513
CviAII CATG 3 cut(s) 449, 669, 709
CviJI RGCY 8 cut(s) 224, 365, 576, 667, 810, 860, 907, 950
CviKI_1 RGCY 8 cut(s) 224, 365, 576, 667, 810, 860, 907, 950
CviQI GTAC 3 cut(s) 149, 270, 513
DdeI CTNAG 5 cut(s) 348, 552, 588, 920, 951
DpnI GATC 4 cut(s) 197, 355, 626, 891
DpnII GATC 4 cut(s) 195, 353, 624, 889
DraIII CACNNNGTG 1 cut(s) 871
Eco130I CCWWGG 2 cut(s) 668, 908
Eco47I GGWCC 3 cut(s) 674, 800, 901
Eco81I CCTNAGG 1 cut(s) 348
EcoRII CCWGG 2 cut(s) 121, 243
EcoT14I CCWWGG 2 cut(s) 668, 908
ErhI CCWWGG 2 cut(s) 668, 908
Esp3I CGTCTC 2 cut(s) 119, 591
FaeI CATG 3 cut(s) 452, 672, 712
FaqI GGGAC 2 cut(s) 277, 687
FatI CATG 3 cut(s) 448, 668, 708
FauI CCCGC 1 cut(s) 638
FbaI TGATCA 1 cut(s) 624
FokI GGATG 1 cut(s) 863
FspBI CTAG 1 cut(s) 252
GlaI GCGC 1 cut(s) 397
HaeIII GGCC 2 cut(s) 667, 907
HapII CCGG 4 cut(s) 180, 191, 803, 904
HgaI GACGC 3 cut(s) 74, 237, 722
HhaI GCGC 1 cut(s) 398
Hin1I GRCGYC 1 cut(s) 248
Hin1II CATG 3 cut(s) 452, 672, 712
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HinfI GANTC 7 cut(s) 10, 74, 109, 183, 255, 378, 593
HpaII CCGG 4 cut(s) 180, 191, 803, 904
HphI GGTGA 4 cut(s) 111, 180, 233, 314
Hpy188I TCNGA 4 cut(s) 642, 662, 725, 820
Hpy188III TCNNGA 7 cut(s) 42, 62, 252, 347, 590, 837, 893
HpyAV CCTTC 3 cut(s) 184, 293, 657
HpyCH4III ACNGT 5 cut(s) 106, 202, 392, 473, 512
HpyCH4IV ACGT 2 cut(s) 272, 823
HpyCH4V TGCA 5 cut(s) 99, 317, 386, 546, 874
HpyF3I CTNAG 5 cut(s) 348, 552, 588, 920, 951
HpySE526I ACGT 2 cut(s) 272, 823
Hsp92I GRCGYC 1 cut(s) 248
Hsp92II CATG 3 cut(s) 452, 672, 712
HspAI GCGC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 624
Kzo9I GATC 4 cut(s) 195, 353, 624, 889
LmnI GCTCC 1 cut(s) 349
LweI GCATC 2 cut(s) 247, 883
MaeI CTAG 1 cut(s) 252
MaeII ACGT 2 cut(s) 272, 823
MaeIII GTNAC 3 cut(s) 89, 379, 473
MalI GATC 4 cut(s) 197, 355, 626, 891
MbiI CCGCTC 2 cut(s) 518, 645
MboI GATC 4 cut(s) 195, 353, 624, 889
MboII GAAGA 2 cut(s) 172, 317
MfeI CAATTG 1 cut(s) 69
MflI RGATCY 1 cut(s) 889
MluCI AATT 3 cut(s) 69, 163, 406
MluI ACGCGT 1 cut(s) 714
MlyI GAGTC 3 cut(s) 4, 264, 387
MnlI CCTC 4 cut(s) 150, 343, 475, 678
MroXI GAANNNNTTC 2 cut(s) 164, 825
MseI TTAA 1 cut(s) 614
MslI CAYNNNNRTG 1 cut(s) 707
MspCI CTTAAG 1 cut(s) 613
MspI CCGG 4 cut(s) 180, 191, 803, 904
MspR9I CCNGG 3 cut(s) 123, 245, 804
MunI CAATTG 1 cut(s) 69
MvaI CCWGG 2 cut(s) 123, 245
MvnI CGCG 1 cut(s) 716
NciI CCSGG 1 cut(s) 804
NcoI CCATGG 1 cut(s) 668
NdeII GATC 4 cut(s) 195, 353, 624, 889
NlaIII CATG 3 cut(s) 452, 672, 712
NlaIV GGNNCC 2 cut(s) 81, 675
NmuCI GTSAC 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PdmI GAANNNNTTC 2 cut(s) 164, 825
PfeI GAWTC 4 cut(s) 74, 109, 183, 593
PleI GAGTC 3 cut(s) 4, 263, 386
PpsI GAGTC 3 cut(s) 4, 263, 386
PsiI TTATAA 2 cut(s) 405, 563
Psp6I CCWGG 2 cut(s) 121, 243
PspGI CCWGG 2 cut(s) 121, 243
PspN4I GGNNCC 2 cut(s) 81, 675
PspPI GGNCC 3 cut(s) 674, 800, 901
PstI CTGCAG 1 cut(s) 388
PsuI RGATCY 1 cut(s) 889
RsaI GTAC 3 cut(s) 150, 271, 514
RsaNI GTAC 3 cut(s) 149, 270, 513
RseI CAYNNNNRTG 1 cut(s) 707
SaqAI TTAA 1 cut(s) 614
Sau3AI GATC 4 cut(s) 195, 353, 624, 889
Sau96I GGNCC 3 cut(s) 674, 800, 901
SchI GAGTC 3 cut(s) 4, 264, 387
ScrFI CCNGG 3 cut(s) 123, 245, 804
SfaNI GCATC 2 cut(s) 247, 883
SfcI CTRYAG 1 cut(s) 384
SinI GGWCC 3 cut(s) 674, 800, 901
SmiMI CAYNNNNRTG 1 cut(s) 707
SmlI CTYRAG 1 cut(s) 613
SmoI CTYRAG 1 cut(s) 613
Sse9I AATT 3 cut(s) 69, 163, 406
SsiI CCGC 2 cut(s) 516, 645
SspI AATATT 1 cut(s) 461
SspMI CTAG 1 cut(s) 252
StyD4I CCNGG 3 cut(s) 121, 243, 802
StyI CCWWGG 2 cut(s) 668, 908
TaaI ACNGT 5 cut(s) 106, 202, 392, 473, 512
TaiI ACGT 2 cut(s) 275, 826
TaqI TCGA 4 cut(s) 13, 412, 480, 836
TasI AATT 3 cut(s) 69, 163, 406
TfiI GAWTC 4 cut(s) 74, 109, 183, 593
Tru1I TTAA 1 cut(s) 614
Tru9I TTAA 1 cut(s) 614
TscAI CASTG 3 cut(s) 388, 536, 548
TseFI GTSAC 1 cut(s) 379
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 2 cut(s) 157, 487
TspGWI ACGGA 1 cut(s) 819
TspRI CASTG 3 cut(s) 388, 536, 548
Vha464I CTTAAG 1 cut(s) 613
VpaK11BI GGWCC 3 cut(s) 674, 800, 901
XapI RAATTY 1 cut(s) 163
XbaI TCTAGA 1 cut(s) 251
XmnI GAANNNNTTC 2 cut(s) 164, 825
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.