MD17G1053000.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004453
Physical Location & Seq
Forward (+)
4142403 .. 4145587
3185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1053000.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGCGGCCCTCGTCCTCAGGGTGGCGCAGGACGGCTCCGGCGACTTCCGGACGGTGCAGGAGGCCATAGACGCCGTCCCACTCTGCAATACCCGTCGCACCGTGATTCGCGTCGCTCGCGGCATCTACAGGCAGCCGGTGTACGTGCCCAAGACGAAGAACCTCATCACGCTCGCGGGTCTGGCTCCGGAGCTCACTGTGCTCACCTGGAACAACACCGCCACCAAAATCGAGCACCACCAGGCTTCTCGGGTGATTGGGACTGGGACTTTCGGGTGTGGGAGTGCTATTGTGGAAGGAGAGGATTTTATTGCAGAGAACATTACTTTTGAGAACTCCGCTCCTGAGGGTTCAGGTCAAGCTGTGGCAATTAGAGTAACAGCTGATCGATGTGCCTTCTATAACTGCAGGTTCCTTGGATGGCAGGATACCCTGTACTTGCATTATGGAAAACAGTATCTGAAAGATTGTTATGTTGAAGGAAGTGTGGACTTCATTTTTGGCAATAGCACGGCGCTTCTGGAGCATTGTCATATCCACTGTAAGTCAGCAGGTTTCATAACTGCCCAAAGCAGGAAATCTTCCCAGGAAACAACGGGATATGTATTCTTAAGATGTGTAATCACTGGAAATGGAGGGACTTCATATGCATATCTGGGACGACCGTGGGGACCTTTTGGAAGAGTTGTTTTTGCGTATACATTTATGGATGGATGCATCAAACATGTAGGATGGAATAATTGGGGTAAAACAGAGAATGAACGAACTGCTTGCTTTTATGAATACAGGTGTTTTGGACCGGGTAGTTGCCCATCCAAACGGGTGACATGGGCAAGAGAGCTGGTAGATGAGGAAGCAGACCAGTTCCTCCAGCATCGTTTCATTGATCCTGATCCAGGAAGGCCTTGGCTTGCTCAGAGAATGGCCCTAAGAATACCGTATTCTGCCTAG

Protein Analysis

317

Amino Acids

35.32

Weight (kDa)

8.22

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 8 - 296 2.4e-74 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 399, 542
AccBSI CCGCTC 1 cut(s) 341
AccI GTMKAC 1 cut(s) 698
AccII CGCG 3 cut(s) 111, 120, 176
AccIII TCCGGA 2 cut(s) 48, 187
AciI CCGC 5 cut(s) 5, 120, 176, 219, 339
AclWI GGATC 2 cut(s) 881, 887
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 2 cut(s) 143, 437
AfiI CCNNNNNNNGG 3 cut(s) 22, 573, 896
AflII CTTAAG 1 cut(s) 610
AflIII ACRYGT 1 cut(s) 724
AgsI TTSAA 1 cut(s) 479
AjnI CCWGG 4 cut(s) 206, 240, 585, 895
AluBI AGCT 4 cut(s) 193, 362, 383, 841
AluI AGCT 4 cut(s) 193, 362, 383, 841
Alw21I GWGCWC 3 cut(s) 195, 204, 237
AlwI GGATC 2 cut(s) 881, 887
AlwNI CAGNNNCTG 1 cut(s) 460
Ama87I CYCGRG 1 cut(s) 249
Aor13HI TCCGGA 2 cut(s) 48, 187
AoxI GGCC 4 cut(s) 6, 63, 902, 924
ApeKI GCWGC 1 cut(s) 133
ArsI GACNNNNNNTTYG 2 cut(s) 482, 514
AspLEI GCGC 2 cut(s) 28, 517
AspS9I GGNCC 4 cut(s) 7, 671, 797, 925
AsuC2I CCSGG 1 cut(s) 801
AsuHPI GGTGA 3 cut(s) 196, 265, 835
AvaI CYCGRG 1 cut(s) 249
AvaII GGWCC 2 cut(s) 671, 797
AxyI CCTNAGG 2 cut(s) 17, 345
BaeGI GKGCMC 1 cut(s) 150
BanII GRGCYC 1 cut(s) 195
Bbv12I GWGCWC 3 cut(s) 195, 204, 237
BbvI GCAGC 1 cut(s) 145
BccI CCATC 4 cut(s) 414, 704, 726, 820
BceAI ACGGC 3 cut(s) 49, 59, 528
BcgI CGANNNNNNTGC 2 cut(s) 753, 787
BciT130I CCWGG 4 cut(s) 208, 242, 587, 897
BciVI GTATCC 1 cut(s) 421
BcnI CCSGG 1 cut(s) 801
BfaI CTAG 1 cut(s) 949
BfmI CTRYAG 2 cut(s) 127, 406
BfoI RGCGCY 1 cut(s) 518
BfrI CTTAAG 1 cut(s) 610
BfuAI ACCTGC 2 cut(s) 399, 542
BfuI GTATCC 1 cut(s) 421
BisI GCNGC 3 cut(s) 6, 121, 134
BlsI GCNGC 3 cut(s) 7, 122, 135
Bme1390I CCNGG 5 cut(s) 208, 242, 587, 801, 897
Bme18I GGWCC 2 cut(s) 671, 797
BmeT110I CYCGRG 1 cut(s) 249
BmgT120I GGNCC 4 cut(s) 7, 671, 797, 925
BmiI GGNNCC 4 cut(s) 37, 186, 413, 672
BmrFI CCNGG 5 cut(s) 208, 242, 587, 801, 897
BmrI ACTGGG 1 cut(s) 273
BmsI GCATC 4 cut(s) 132, 704, 726, 883
BmuI ACTGGG 1 cut(s) 273
BpmI CTGGAG 2 cut(s) 542, 854
BpuMI CCSGG 1 cut(s) 801
Bsa29I ATCGAT 1 cut(s) 388
BsaAI YACGTR 1 cut(s) 145
BsaBI GATNNNNATC 1 cut(s) 891
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 4 cut(s) 415, 585, 665, 905
BsaWI WCCGGW 2 cut(s) 48, 187
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 573, 896
Bse118I RCCGGY 1 cut(s) 136
Bse1I ACTGG 3 cut(s) 268, 631, 862
Bse21I CCTNAGG 2 cut(s) 17, 345
Bse8I GATNNNNATC 1 cut(s) 891
BseAI TCCGGA 2 cut(s) 48, 187
BseBI CCWGG 4 cut(s) 208, 242, 587, 897
BseCI ATCGAT 1 cut(s) 388
BseDI CCNNGG 4 cut(s) 415, 585, 665, 905
BseGI GGATG 5 cut(s) 425, 715, 719, 737, 812
BseJI GATNNNNATC 1 cut(s) 891
BseLI CCNNNNNNNGG 3 cut(s) 22, 573, 896
BseMII CTCAG 3 cut(s) 31, 336, 929
BseNI ACTGG 3 cut(s) 268, 631, 862
BseSI GKGCMC 1 cut(s) 150
BseXI GCAGC 1 cut(s) 145
BsgI GTGCAG 1 cut(s) 77
Bsh1236I CGCG 3 cut(s) 111, 120, 176
Bsh1285I CGRYCG 1 cut(s) 665
BshFI GGCC 4 cut(s) 8, 65, 904, 926
BshVI ATCGAT 1 cut(s) 388
BsiEI CGRYCG 1 cut(s) 665
BsiHKAI GWGCWC 3 cut(s) 195, 204, 237
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 5 cut(s) 39, 49, 137, 188, 800
BslFI GGGAC 6 cut(s) 62, 274, 280, 652, 672, 684
BslI CCNNNNNNNGG 3 cut(s) 22, 573, 896
BsmFI GGGAC 6 cut(s) 62, 274, 280, 652, 672, 684
BsnI GGCC 4 cut(s) 8, 65, 904, 926
BsoBI CYCGRG 1 cut(s) 249
Bsp1286I GDGCHC 4 cut(s) 150, 195, 204, 237
Bsp13I TCCGGA 2 cut(s) 48, 187
Bsp143I GATC 3 cut(s) 385, 886, 892
BspACI CCGC 5 cut(s) 5, 120, 176, 219, 339
BspANI GGCC 4 cut(s) 8, 65, 904, 926
BspCNI CTCAG 3 cut(s) 30, 337, 928
BspDI ATCGAT 1 cut(s) 388
BspEI TCCGGA 2 cut(s) 48, 187
BspFNI CGCG 3 cut(s) 111, 120, 176
BspLI GGNNCC 4 cut(s) 37, 186, 413, 672
BspMAI CTGCAG 1 cut(s) 410
BspMI ACCTGC 2 cut(s) 399, 542
BspPI GGATC 2 cut(s) 881, 887
BspTI CTTAAG 1 cut(s) 610
BsrBI CCGCTC 1 cut(s) 341
BsrFI RCCGGY 1 cut(s) 136
BsrI ACTGG 3 cut(s) 268, 631, 862
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 4 cut(s) 415, 585, 665, 905
BssMI GATC 3 cut(s) 385, 886, 892
BssNAI GTATAC 1 cut(s) 699
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 2 cut(s) 415, 905
Bst1107I GTATAC 1 cut(s) 699
Bst2UI CCWGG 4 cut(s) 208, 242, 587, 897
Bst4CI ACNGT 7 cut(s) 55, 103, 199, 456, 542, 666, 939
Bst6I CTCTTC 1 cut(s) 676
BstACI GRCGYC 1 cut(s) 72
BstAFI CTTAAG 1 cut(s) 610
BstBAI YACGTR 1 cut(s) 145
BstC8I GCNNGC 4 cut(s) 118, 174, 772, 912
BstDEI CTNAG 4 cut(s) 17, 345, 915, 929
BstDSI CCRYGG 1 cut(s) 665
BstENI CCTNNNNNAGG 1 cut(s) 894
BstF5I GGATG 5 cut(s) 425, 715, 719, 737, 812
BstFNI CGCG 3 cut(s) 111, 120, 176
BstH2I RGCGCY 1 cut(s) 518
BstHHI GCGC 2 cut(s) 28, 517
BstKTI GATC 3 cut(s) 388, 889, 895
BstMBI GATC 3 cut(s) 385, 886, 892
BstMCI CGRYCG 1 cut(s) 665
BstMWI GCNNNNNNNGC 5 cut(s) 71, 117, 182, 199, 523
BstNI CCWGG 4 cut(s) 208, 242, 587, 897
BstNSI RCATGY 1 cut(s) 728
BstSCI CCNGG 5 cut(s) 206, 240, 585, 799, 895
BstSFI CTRYAG 2 cut(s) 127, 406
BstSLI GKGCMC 1 cut(s) 150
BstUI CGCG 3 cut(s) 111, 120, 176
BstV1I GCAGC 1 cut(s) 145
BstZ17I GTATAC 1 cut(s) 699
Bsu15I ATCGAT 1 cut(s) 388
Bsu36I CCTNAGG 2 cut(s) 17, 345
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 4 cut(s) 8, 65, 904, 926
BsuTUI ATCGAT 1 cut(s) 388
BtgI CCRYGG 1 cut(s) 665
BtsCI GGATG 5 cut(s) 425, 715, 719, 737, 812
BtsIMutI CAGTG 3 cut(s) 195, 538, 624
BveI ACCTGC 2 cut(s) 399, 542
Cac8I GCNNGC 4 cut(s) 118, 174, 772, 912
CaiI CAGNNNCTG 1 cut(s) 460
CfoI GCGC 2 cut(s) 28, 517
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 4 cut(s) 7, 671, 797, 925
ClaI ATCGAT 1 cut(s) 388
CseI GACGC 2 cut(s) 80, 100
Csp6I GTAC 2 cut(s) 142, 436
CviAII CATG 2 cut(s) 725, 828
CviQI GTAC 2 cut(s) 142, 436
DdeI CTNAG 4 cut(s) 17, 345, 915, 929
DpnI GATC 3 cut(s) 387, 888, 894
DpnII GATC 3 cut(s) 385, 886, 892
Eam1104I CTCTTC 1 cut(s) 676
EarI CTCTTC 1 cut(s) 676
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 2 cut(s) 415, 905
Eco147I AGGCCT 1 cut(s) 904
Eco24I GRGCYC 1 cut(s) 195
Eco47I GGWCC 2 cut(s) 671, 797
Eco53kI GAGCTC 1 cut(s) 193
Eco81I CCTNAGG 2 cut(s) 17, 345
Eco88I CYCGRG 1 cut(s) 249
EcoICRI GAGCTC 1 cut(s) 193
EcoNI CCTNNNNNAGG 1 cut(s) 894
EcoO109I RGGNCCY 1 cut(s) 671
EcoRII CCWGG 4 cut(s) 206, 240, 585, 895
EcoT14I CCWWGG 2 cut(s) 415, 905
EcoT22I ATGCAT 2 cut(s) 652, 719
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 2 cut(s) 415, 905
FaeI CATG 2 cut(s) 728, 831
FaqI GGGAC 6 cut(s) 62, 274, 280, 652, 672, 684
FatI CATG 2 cut(s) 724, 827
FauI CCCGC 1 cut(s) 169
FauNDI CATATG 1 cut(s) 646
FblI GTMKAC 1 cut(s) 698
Fnu4HI GCNGC 3 cut(s) 6, 121, 134
FokI GGATG 5 cut(s) 432, 722, 726, 744, 799
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 3 cut(s) 6, 121, 134
FspBI CTAG 1 cut(s) 949
GlaI GCGC 2 cut(s) 27, 516
GluI GCNGC 3 cut(s) 6, 121, 134
GsuI CTGGAG 2 cut(s) 542, 854
HaeII RGCGCY 1 cut(s) 518
HaeIII GGCC 4 cut(s) 8, 65, 904, 926
HapII CCGG 5 cut(s) 39, 49, 137, 188, 800
HgaI GACGC 2 cut(s) 80, 100
HhaI GCGC 2 cut(s) 28, 517
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 2 cut(s) 728, 831
Hin6I GCGC 2 cut(s) 26, 515
HinP1I GCGC 2 cut(s) 26, 515
HinfI GANTC 1 cut(s) 106
HpaII CCGG 5 cut(s) 39, 49, 137, 188, 800
HphI GGTGA 3 cut(s) 196, 265, 835
Hpy166II GTNNAC 3 cut(s) 142, 490, 699
Hpy188I TCNGA 2 cut(s) 462, 918
Hpy188III TCNNGA 5 cut(s) 49, 188, 344, 521, 890
Hpy8I GTNNAC 3 cut(s) 142, 490, 699
Hpy99I CGWCG 2 cut(s) 99, 116
HpyAV CCTTC 4 cut(s) 290, 406, 473, 894
HpyCH4III ACNGT 7 cut(s) 55, 103, 199, 456, 542, 666, 939
HpyCH4IV ACGT 1 cut(s) 144
HpyCH4V TGCA 7 cut(s) 58, 87, 314, 408, 442, 650, 717
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 117, 182, 199, 523
HpyF3I CTNAG 4 cut(s) 17, 345, 915, 929
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 2 cut(s) 728, 831
HspAI GCGC 2 cut(s) 26, 515
Kpn2I TCCGGA 2 cut(s) 48, 187
Kzo9I GATC 3 cut(s) 385, 886, 892
LmnI GCTCC 5 cut(s) 41, 190, 190, 346, 523
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 4 cut(s) 132, 704, 726, 883
MaeI CTAG 1 cut(s) 949
MaeII ACGT 1 cut(s) 144
MaeIII GTNAC 2 cut(s) 376, 823
MalI GATC 3 cut(s) 387, 888, 894
MbiI CCGCTC 1 cut(s) 341
MboI GATC 3 cut(s) 385, 886, 892
MboII GAAGA 3 cut(s) 169, 573, 693
MhlI GDGCHC 4 cut(s) 150, 195, 204, 237
MluCI AATT 2 cut(s) 369, 739
MnlI CCTC 9 cut(s) 20, 26, 55, 173, 295, 340, 629, 844, 878
Mph1103I ATGCAT 2 cut(s) 652, 719
MroI TCCGGA 2 cut(s) 48, 187
MseI TTAA 1 cut(s) 611
MspA1I CMGCKG 1 cut(s) 383
MspCI CTTAAG 1 cut(s) 610
MspI CCGG 5 cut(s) 39, 49, 137, 188, 800
MspR9I CCNGG 5 cut(s) 208, 242, 587, 801, 897
MvaI CCWGG 4 cut(s) 208, 242, 587, 897
MvnI CGCG 3 cut(s) 111, 120, 176
MwoI GCNNNNNNNGC 5 cut(s) 71, 117, 182, 199, 523
NciI CCSGG 1 cut(s) 801
NdeI CATATG 1 cut(s) 646
NdeII GATC 3 cut(s) 385, 886, 892
NlaIII CATG 2 cut(s) 728, 831
NlaIV GGNNCC 4 cut(s) 37, 186, 413, 672
NmuCI GTSAC 1 cut(s) 823
NsiI ATGCAT 2 cut(s) 652, 719
NspI RCATGY 1 cut(s) 728
PceI AGGCCT 1 cut(s) 904
PciI ACATGT 1 cut(s) 724
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PfeI GAWTC 1 cut(s) 106
PflFI GACNNNGTC 1 cut(s) 74
PfoI TCCNGGA 1 cut(s) 895
PkrI GCNGC 3 cut(s) 7, 122, 135
Ppu21I YACGTR 1 cut(s) 145
PpuMI RGGWCCY 1 cut(s) 671
PscI ACATGT 1 cut(s) 724
Psp124BI GAGCTC 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 671
Psp6I CCWGG 4 cut(s) 206, 240, 585, 895
PspGI CCWGG 4 cut(s) 206, 240, 585, 895
PspN4I GGNNCC 4 cut(s) 37, 186, 413, 672
PspPI GGNCC 4 cut(s) 7, 671, 797, 925
PspPPI RGGWCCY 1 cut(s) 671
PstI CTGCAG 1 cut(s) 410
PstNI CAGNNNCTG 1 cut(s) 460
PsyI GACNNNGTC 1 cut(s) 74
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 2 cut(s) 143, 437
RsaNI GTAC 2 cut(s) 142, 436
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 1 cut(s) 611
SatI GCNGC 3 cut(s) 6, 121, 134
Sau3AI GATC 3 cut(s) 385, 886, 892
Sau96I GGNCC 4 cut(s) 7, 671, 797, 925
ScrFI CCNGG 5 cut(s) 208, 242, 587, 801, 897
SduI GDGCHC 4 cut(s) 150, 195, 204, 237
SfaNI GCATC 4 cut(s) 132, 704, 726, 883
SfcI CTRYAG 2 cut(s) 127, 406
SinI GGWCC 2 cut(s) 671, 797
SmlI CTYRAG 1 cut(s) 610
SmoI CTYRAG 1 cut(s) 610
Sse9I AATT 2 cut(s) 369, 739
SseBI AGGCCT 1 cut(s) 904
SsiI CCGC 5 cut(s) 5, 120, 176, 219, 339
SspMI CTAG 1 cut(s) 949
SstI GAGCTC 1 cut(s) 195
StuI AGGCCT 1 cut(s) 904
StyD4I CCNGG 5 cut(s) 206, 240, 585, 799, 895
StyI CCWWGG 2 cut(s) 415, 905
TaaI ACNGT 7 cut(s) 55, 103, 199, 456, 542, 666, 939
TaiI ACGT 1 cut(s) 147
TaqI TCGA 2 cut(s) 231, 388
TasI AATT 2 cut(s) 369, 739
TatI WGTACW 1 cut(s) 435
TauI GCSGC 2 cut(s) 8, 123
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 1 cut(s) 611
Tru9I TTAA 1 cut(s) 611
TscAI CASTG 3 cut(s) 202, 545, 631
TseFI GTSAC 1 cut(s) 823
TseI GCWGC 1 cut(s) 133
Tsp45I GTSAC 1 cut(s) 823
TspDTI ATGAA 6 cut(s) 484, 547, 633, 774, 795, 871
TspRI CASTG 3 cut(s) 202, 545, 631
Tth111I GACNNNGTC 1 cut(s) 74
Vha464I CTTAAG 1 cut(s) 610
VpaK11BI GGWCC 2 cut(s) 671, 797
XagI CCTNNNNNAGG 1 cut(s) 894
XceI RCATGY 1 cut(s) 728
XcmI CCANNNNNNNNNTGG 1 cut(s) 903
XmiI GTMKAC 1 cut(s) 698
XspI CTAG 1 cut(s) 949
Zsp2I ATGCAT 2 cut(s) 652, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.