RLG00000021733

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004453
Physical Location & Seq
Forward (+)
77635123 .. 77637934
2812 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021733

Sequence Viewer

Length: 936 bp
ATGAACCTTTTTAATGTCGGCCTATGTGATAACCGTGTCACAGGACGGCTCCACCGCACCGTACAGGAGGCCATCGATGCCGTGCCTCTCTGCAACACGTGTCGCACTGTGATTCGCATAGCTCCCGGCATCTACCGGCAGCCGGTGTATGTCCCCAAGACCAAGAACCTCATCACACTCGCGGGTCTGCGACCGGAGGAGACTGTGCTGACGTGGAACAACACCGCCACCAAAATCGAGCACCACCAGGGTGATTGGGACTGGGACTGGAGTACAATTGTGGAAGGAGAGGACTTCATTGCTGAGAATATCACTTTTGAGAACTCTTCTCCTGAGGTAAAGCTCACTGTGGCAATTAGAGTAACAGCTGATCGGTGTGCCTTCTATAACTGCAGATTCCTTGGATGGCAGGATACCCTGTACTTACATTATGGAAAGCAGTATCTGAAAGATTGTTATATTGAAGGAAGTGTAGACTTCATTTTCGGTAATAGCACAGCTCTTTTGGAACATTGTCATATCCACTGCAAGTCAGCAGGTTTCATAACTGCGCAAAGCAGGAAATCATCCCAGGAGACTACAGGATATGTGTTCCTAAGATGTGTAATAACTGGAAATGGAGGTACTCCATACGCATTTCTGGGACGACCATGGGGACCTTTTGGAAGAGTGGTCTTTGCATTTACATATATGGATGCATGCATCAAACATGTAGGCTGGAATAATTGGGGTAAAACAGAGAATGAAAGAAGCACTTGCTTTTACGAATACAGGTGTTTTGGACCGGCTAGTTGCCCATCAAAACGGGTGACATGGGCTAGAGAACTGGTAGATGAGGAAGCAGACCAGTTCCTCAAGCATGGCTACATCGGCCCAGACCACCAAAGACCTTGGCTTGCTCAGAGAATGGCCCTAAGAATACCATATTCTGCATAG

Protein Analysis

312

Amino Acids

35.59

Weight (kDa)

7.53

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 18 - 290 8.2e-63 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 552
Acc36I ACCTGC 1 cut(s) 527
AccI GTMKAC 1 cut(s) 474
AccII CGCG 1 cut(s) 182
AciI CCGC 3 cut(s) 55, 182, 225
AcvI CACGTG 1 cut(s) 99
AfaI GTAC 4 cut(s) 63, 274, 422, 625
AfiI CCNNNNNNNGG 1 cut(s) 142
AflIII ACRYGT 3 cut(s) 96, 98, 709
AgsI TTSAA 1 cut(s) 464
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 2 cut(s) 246, 570
AleI CACNNNNGTG 1 cut(s) 249
AluBI AGCT 4 cut(s) 122, 343, 368, 500
AluI AGCT 4 cut(s) 122, 343, 368, 500
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 2 cut(s) 194, 569
AlwNI CAGNNNCTG 1 cut(s) 445
AoxI GGCC 4 cut(s) 19, 69, 871, 909
ApeKI GCWGC 1 cut(s) 139
ArsI GACNNNNNNTTYG 2 cut(s) 467, 499
AspLEI GCGC 1 cut(s) 553
AspS9I GGNCC 4 cut(s) 656, 782, 872, 910
AsuC2I CCSGG 1 cut(s) 126
AsuHPI GGTGA 2 cut(s) 263, 820
AvaII GGWCC 2 cut(s) 656, 782
AxyI CCTNAGG 1 cut(s) 333
BbrPI CACGTG 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 151
BccI CCATC 3 cut(s) 80, 399, 805
BceAI ACGGC 2 cut(s) 62, 65
BciT130I CCWGG 2 cut(s) 248, 572
BciVI GTATCC 1 cut(s) 406
BcnI CCSGG 1 cut(s) 126
BcoDI GTCTC 2 cut(s) 194, 569
BfaI CTAG 2 cut(s) 789, 819
BfmI CTRYAG 2 cut(s) 391, 579
BfuAI ACCTGC 1 cut(s) 527
BfuI GTATCC 1 cut(s) 406
BisI GCNGC 1 cut(s) 140
BlsI GCNGC 1 cut(s) 141
Bme1390I CCNGG 3 cut(s) 126, 248, 572
Bme18I GGWCC 2 cut(s) 656, 782
BmgBI CACGTC 1 cut(s) 213
BmgT120I GGNCC 4 cut(s) 656, 782, 872, 910
BmiI GGNNCC 2 cut(s) 50, 657
BmrFI CCNGG 3 cut(s) 126, 248, 572
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 4 cut(s) 67, 138, 685, 711
BmuI ACTGGG 1 cut(s) 271
BpmI CTGGAG 1 cut(s) 289
BpuEI CTTGAG 1 cut(s) 839
BpuMI CCSGG 1 cut(s) 126
Bsa29I ATCGAT 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 99
BsaJI CCNNGG 5 cut(s) 247, 400, 570, 650, 890
BsaWI WCCGGW 1 cut(s) 193
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse118I RCCGGY 3 cut(s) 135, 142, 784
Bse1I ACTGG 5 cut(s) 266, 272, 616, 831, 847
Bse21I CCTNAGG 1 cut(s) 333
Bse3DI GCAATG 1 cut(s) 297
BseBI CCWGG 2 cut(s) 248, 572
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 5 cut(s) 247, 400, 570, 650, 890
BseGI GGATG 3 cut(s) 410, 566, 700
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMI GCAATG 1 cut(s) 297
BseMII CTCAG 3 cut(s) 294, 324, 914
BseNI ACTGG 5 cut(s) 266, 272, 616, 831, 847
BseRI GAGGAG 1 cut(s) 212
BseXI GCAGC 1 cut(s) 151
Bsh1236I CGCG 1 cut(s) 182
Bsh1285I CGRYCG 1 cut(s) 194
BshFI GGCC 4 cut(s) 21, 71, 873, 911
BshVI ATCGAT 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 194
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 5 cut(s) 126, 136, 143, 194, 785
BslFI GGGAC 5 cut(s) 137, 272, 278, 657, 669
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 2 cut(s) 194, 569
BsmFI GGGAC 5 cut(s) 137, 272, 278, 657, 669
BsnI GGCC 4 cut(s) 21, 71, 873, 911
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 1 cut(s) 370
Bsp19I CCATGG 1 cut(s) 650
BspACI CCGC 3 cut(s) 55, 182, 225
BspANI GGCC 4 cut(s) 21, 71, 873, 911
BspCNI CTCAG 3 cut(s) 295, 325, 913
BspDI ATCGAT 1 cut(s) 75
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 2 cut(s) 50, 657
BspMAI CTGCAG 1 cut(s) 395
BspMI ACCTGC 1 cut(s) 527
BsrDI GCAATG 1 cut(s) 297
BsrFI RCCGGY 3 cut(s) 135, 142, 784
BsrI ACTGG 5 cut(s) 266, 272, 616, 831, 847
BssAI RCCGGY 3 cut(s) 135, 142, 784
BssECI CCNNGG 5 cut(s) 247, 400, 570, 650, 890
BssMI GATC 1 cut(s) 370
BssT1I CCWWGG 3 cut(s) 400, 650, 890
Bst2UI CCWGG 2 cut(s) 248, 572
Bst4CI ACNGT 5 cut(s) 35, 61, 109, 205, 349
Bst6I CTCTTC 2 cut(s) 331, 661
BstBAI YACGTR 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 700, 897
BstDEI CTNAG 5 cut(s) 303, 333, 596, 900, 914
BstDSI CCRYGG 1 cut(s) 650
BstF5I GGATG 3 cut(s) 410, 566, 700
BstFNI CGCG 1 cut(s) 182
BstHHI GCGC 1 cut(s) 553
BstKTI GATC 1 cut(s) 373
BstMAI GTCTC 2 cut(s) 194, 569
BstMBI GATC 1 cut(s) 370
BstMCI CGRYCG 1 cut(s) 194
BstMWI GCNNNNNNNGC 2 cut(s) 77, 870
BstNI CCWGG 2 cut(s) 248, 572
BstNSI RCATGY 2 cut(s) 702, 713
BstSCI CCNGG 3 cut(s) 124, 246, 570
BstSFI CTRYAG 2 cut(s) 391, 579
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 151
Bsu15I ATCGAT 1 cut(s) 75
Bsu36I CCTNAGG 1 cut(s) 333
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 4 cut(s) 21, 71, 873, 911
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 1 cut(s) 650
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 3 cut(s) 410, 566, 700
BtsI GCAGTG 1 cut(s) 523
BtsIMutI CAGTG 3 cut(s) 105, 345, 523
BveI ACCTGC 1 cut(s) 527
Cac8I GCNNGC 2 cut(s) 700, 897
CaiI CAGNNNCTG 1 cut(s) 445
CfoI GCGC 1 cut(s) 553
Cfr10I RCCGGY 3 cut(s) 135, 142, 784
Cfr13I GGNCC 4 cut(s) 656, 782, 872, 910
ClaI ATCGAT 1 cut(s) 75
Csp6I GTAC 4 cut(s) 62, 273, 421, 624
CviAII CATG 5 cut(s) 651, 699, 710, 813, 860
CviQI GTAC 4 cut(s) 62, 273, 421, 624
DdeI CTNAG 5 cut(s) 303, 333, 596, 900, 914
DpnI GATC 1 cut(s) 372
DpnII GATC 1 cut(s) 370
Eam1104I CTCTTC 2 cut(s) 331, 661
EarI CTCTTC 2 cut(s) 331, 661
Eco130I CCWWGG 3 cut(s) 400, 650, 890
Eco47I GGWCC 2 cut(s) 656, 782
Eco72I CACGTG 1 cut(s) 99
Eco81I CCTNAGG 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 656
EcoRII CCWGG 2 cut(s) 246, 570
EcoT14I CCWWGG 3 cut(s) 400, 650, 890
EcoT22I ATGCAT 2 cut(s) 700, 704
ErhI CCWWGG 3 cut(s) 400, 650, 890
FaeI CATG 5 cut(s) 654, 702, 713, 816, 863
FalI AAGNNNNNCTT 2 cut(s) 739, 771
FaqI GGGAC 5 cut(s) 137, 272, 278, 657, 669
FatI CATG 5 cut(s) 650, 698, 709, 812, 859
FauI CCCGC 1 cut(s) 175
FblI GTMKAC 1 cut(s) 474
Fnu4HI GCNGC 1 cut(s) 140
FokI GGATG 3 cut(s) 417, 553, 707
Fsp4HI GCNGC 1 cut(s) 140
FspBI CTAG 2 cut(s) 789, 819
FspI TGCGCA 1 cut(s) 552
GlaI GCGC 1 cut(s) 552
GluI GCNGC 1 cut(s) 140
GsuI CTGGAG 1 cut(s) 289
HaeIII GGCC 4 cut(s) 21, 71, 873, 911
HapII CCGG 5 cut(s) 126, 136, 143, 194, 785
HhaI GCGC 1 cut(s) 553
Hin1II CATG 5 cut(s) 654, 702, 713, 816, 863
Hin6I GCGC 1 cut(s) 551
HinP1I GCGC 1 cut(s) 551
HinfI GANTC 2 cut(s) 112, 396
HpaII CCGG 5 cut(s) 126, 136, 143, 194, 785
HphI GGTGA 2 cut(s) 263, 820
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 2 cut(s) 447, 903
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 1 cut(s) 475
HpyAV CCTTC 3 cut(s) 278, 391, 458
HpyCH4III ACNGT 5 cut(s) 35, 61, 109, 205, 349
HpyCH4IV ACGT 2 cut(s) 98, 212
HpyCH4V TGCA 7 cut(s) 93, 393, 528, 680, 698, 702, 932
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 870
HpyF3I CTNAG 5 cut(s) 303, 333, 596, 900, 914
HpySE526I ACGT 2 cut(s) 98, 212
Hsp92II CATG 5 cut(s) 654, 702, 713, 816, 863
HspAI GCGC 1 cut(s) 551
Kzo9I GATC 1 cut(s) 370
LmnI GCTCC 2 cut(s) 54, 127
Lsp1109I GCAGC 1 cut(s) 151
LweI GCATC 4 cut(s) 67, 138, 685, 711
MaeI CTAG 2 cut(s) 789, 819
MaeII ACGT 2 cut(s) 98, 212
MaeIII GTNAC 3 cut(s) 37, 361, 808
MalI GATC 1 cut(s) 372
MboI GATC 1 cut(s) 370
MboII GAAGA 2 cut(s) 318, 678
MfeI CAATTG 1 cut(s) 276
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 3 cut(s) 276, 354, 724
MnlI CCTC 9 cut(s) 61, 96, 179, 190, 283, 328, 614, 829, 863
Mph1103I ATGCAT 2 cut(s) 700, 704
MseI TTAA 1 cut(s) 12
MslI CAYNNNNRTG 1 cut(s) 249
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 5 cut(s) 126, 136, 143, 194, 785
MspR9I CCNGG 3 cut(s) 126, 248, 572
MunI CAATTG 1 cut(s) 276
MvaI CCWGG 2 cut(s) 248, 572
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 2 cut(s) 77, 870
NciI CCSGG 1 cut(s) 126
NcoI CCATGG 1 cut(s) 650
NdeII GATC 1 cut(s) 370
NlaIII CATG 5 cut(s) 654, 702, 713, 816, 863
NlaIV GGNNCC 2 cut(s) 50, 657
NmuCI GTSAC 2 cut(s) 37, 808
NsbI TGCGCA 1 cut(s) 552
NsiI ATGCAT 2 cut(s) 700, 704
NspI RCATGY 2 cut(s) 702, 713
OliI CACNNNNGTG 1 cut(s) 249
PaeI GCATGC 1 cut(s) 702
PciI ACATGT 1 cut(s) 709
PfeI GAWTC 2 cut(s) 112, 396
PkrI GCNGC 1 cut(s) 141
PmaCI CACGTG 1 cut(s) 99
PmlI CACGTG 1 cut(s) 99
Ppu21I YACGTR 1 cut(s) 99
PpuMI RGGWCCY 1 cut(s) 656
PscI ACATGT 1 cut(s) 709
Psp5II RGGWCCY 1 cut(s) 656
Psp6I CCWGG 2 cut(s) 246, 570
PspCI CACGTG 1 cut(s) 99
PspGI CCWGG 2 cut(s) 246, 570
PspN4I GGNNCC 2 cut(s) 50, 657
PspPI GGNCC 4 cut(s) 656, 782, 872, 910
PspPPI RGGWCCY 1 cut(s) 656
PstI CTGCAG 1 cut(s) 395
PstNI CAGNNNCTG 1 cut(s) 445
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 4 cut(s) 63, 274, 422, 625
RsaNI GTAC 4 cut(s) 62, 273, 421, 624
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 1 cut(s) 140
Sau3AI GATC 1 cut(s) 370
Sau96I GGNCC 4 cut(s) 656, 782, 872, 910
ScrFI CCNGG 3 cut(s) 126, 248, 572
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 4 cut(s) 67, 138, 685, 711
SfcI CTRYAG 2 cut(s) 391, 579
SinI GGWCC 2 cut(s) 656, 782
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
SphI GCATGC 1 cut(s) 702
Sse9I AATT 3 cut(s) 276, 354, 724
SsiI CCGC 3 cut(s) 55, 182, 225
SspMI CTAG 2 cut(s) 789, 819
StyD4I CCNGG 3 cut(s) 124, 246, 570
StyI CCWWGG 3 cut(s) 400, 650, 890
TaaI ACNGT 5 cut(s) 35, 61, 109, 205, 349
TaiI ACGT 2 cut(s) 101, 215
TaqI TCGA 2 cut(s) 75, 237
TasI AATT 3 cut(s) 276, 354, 724
TatI WGTACW 2 cut(s) 272, 420
TfiI GAWTC 2 cut(s) 112, 396
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TscAI CASTG 3 cut(s) 112, 352, 530
TseFI GTSAC 2 cut(s) 37, 808
TseI GCWGC 1 cut(s) 139
Tsp45I GTSAC 2 cut(s) 37, 808
TspDTI ATGAA 5 cut(s) 17, 286, 469, 532, 759
TspRI CASTG 3 cut(s) 112, 352, 530
VpaK11BI GGWCC 2 cut(s) 656, 782
XceI RCATGY 2 cut(s) 702, 713
XmiI GTMKAC 1 cut(s) 474
XspI CTAG 2 cut(s) 789, 819
Zsp2I ATGCAT 2 cut(s) 700, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.