Rw2G049630

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004453
Physical Location & Seq
Reverse (-)
77237698 .. 77241100
3403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G049630.1

Sequence Viewer

Length: 951 bp
ATGTCGGCCTATGTGATAACCGTGTCACAGGACGGCTCCGGTGACCACCGCACCGTTCAGGAGGCCATCGACGCCGTGCCTCTCTGCAACACGTGTCGCACTGTGATTCGTATAGCTCCCGGCATCTACCGGCAGCCGGTGTATGTCCCCAAGACCAAGAACCTCATCACACTCGCGGGTCTGCGACCGGAGGGTACTGTGCTGACGTGGAACAACACCGCCACCAAAATCGAGCACCACCAGGCTTCTAGGGTGATTGGGACTGGGACTTTTGGATGTGGGAGTACAATTGTGGAAGGAGAGGACTTCATTGCTGAGAATATCACTTTTGAGAACTCTTCTCCTGAGGGTTCGGGCCAAGCTGTGGCAATTAGAGTAACGGCTGATCGGTGCGCCTTCTATAACTGCAGATTCCTTGGATGGCAGGATACCCTGTACTTGCATTATGGAAAGCAGTATTTGAAAGATTGTTATATTGAAGGAAGTGTAGACTTCATTTTCGGTAATAGCACAGCTCTTTTGGAGCATTGTCATATCCACTGCAAGTCAGCAGGTTTCATAACTGCGCAAAGCAGGAAATCATCCCAGGAGACTACAGGATATGTGTTCCTAAGGTGTGTAATAACTGGAAATGGAGGTGCTCCATACGCATTTCTGGGACGACCATGGGGACCTTTTGGAAGAGTGGTCTTTGCATTTACATATATGGATGCATGCATCAAACATGTAGGCTGGAATAATTGGGGTAAAACAGAGAATGAAAGAAGCGCTTGCTTTTACGAATACAGGTGTTTTGGACCGGGTAGTTGCCCATCAAAACGAGTGACATGGGCTAGAGAACTGGTAGATGAGGAAGCAGACCAGTTCCTCAAGCATGGTTACATCGACCCAGACCCCCAAAGACCTTGGCTTGCTCAGAGAATGGCCCTAAGAATACCATATTCTGCATAG

Protein Analysis

316

Amino Acids

35.28

Weight (kDa)

7.52

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 6 - 296 3.3e-70 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 567
Acc36I ACCTGC 1 cut(s) 542
AccB7I CCANNNNNTGG 1 cut(s) 364
AccI GTMKAC 1 cut(s) 489
AccII CGCG 1 cut(s) 176
AciI CCGC 3 cut(s) 49, 176, 219
AcvI CACGTG 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 3 cut(s) 196, 286, 437
AfeI AGCGCT 1 cut(s) 769
AfiI CCNNNNNNNGG 2 cut(s) 136, 364
AflIII ACRYGT 3 cut(s) 90, 92, 724
AgsI TTSAA 2 cut(s) 463, 479
AjiI CACGTC 1 cut(s) 207
AjnI CCWGG 2 cut(s) 240, 585
AluBI AGCT 3 cut(s) 116, 362, 515
AluI AGCT 3 cut(s) 116, 362, 515
Alw21I GWGCWC 2 cut(s) 237, 643
Alw26I GTCTC 1 cut(s) 584
Aor51HI AGCGCT 1 cut(s) 769
AoxI GGCC 4 cut(s) 6, 63, 355, 924
ApeKI GCWGC 1 cut(s) 133
ArsI GACNNNNNNTTYG 2 cut(s) 482, 514
AspLEI GCGC 3 cut(s) 395, 568, 770
AspS9I GGNCC 4 cut(s) 355, 671, 797, 925
AsuC2I CCSGG 2 cut(s) 120, 801
AsuHPI GGTGA 2 cut(s) 53, 265
AvaII GGWCC 2 cut(s) 671, 797
AxyI CCTNAGG 2 cut(s) 345, 611
BbrPI CACGTG 1 cut(s) 93
Bbv12I GWGCWC 2 cut(s) 237, 643
BbvI GCAGC 1 cut(s) 145
BccI CCATC 3 cut(s) 74, 414, 820
BceAI ACGGC 3 cut(s) 49, 59, 396
BciT130I CCWGG 2 cut(s) 242, 587
BciVI GTATCC 1 cut(s) 421
BcnI CCSGG 2 cut(s) 120, 801
BcoDI GTCTC 1 cut(s) 584
BfaI CTAG 2 cut(s) 249, 834
BfmI CTRYAG 2 cut(s) 406, 594
BfoI RGCGCY 1 cut(s) 771
BfuAI ACCTGC 1 cut(s) 542
BfuI GTATCC 1 cut(s) 421
BisI GCNGC 1 cut(s) 134
BlsI GCNGC 1 cut(s) 135
Bme1390I CCNGG 4 cut(s) 120, 242, 587, 801
Bme18I GGWCC 2 cut(s) 671, 797
BmgBI CACGTC 1 cut(s) 207
BmgT120I GGNCC 4 cut(s) 355, 671, 797, 925
BmiI GGNNCC 2 cut(s) 37, 672
BmrFI CCNGG 4 cut(s) 120, 242, 587, 801
BmrI ACTGGG 1 cut(s) 273
BmsI GCATC 3 cut(s) 132, 700, 726
BmuI ACTGGG 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 854
BpuMI CCSGG 2 cut(s) 120, 801
BsaAI YACGTR 1 cut(s) 93
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 4 cut(s) 415, 585, 665, 905
BsaWI WCCGGW 2 cut(s) 38, 187
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 364
Bse118I RCCGGY 2 cut(s) 129, 136
Bse1I ACTGG 4 cut(s) 268, 631, 846, 862
Bse21I CCTNAGG 2 cut(s) 345, 611
Bse3DI GCAATG 1 cut(s) 309
BseBI CCWGG 2 cut(s) 242, 587
BseDI CCNNGG 4 cut(s) 415, 585, 665, 905
BseGI GGATG 4 cut(s) 281, 425, 581, 715
BseLI CCNNNNNNNGG 2 cut(s) 136, 364
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 3 cut(s) 306, 336, 929
BseNI ACTGG 4 cut(s) 268, 631, 846, 862
BseXI GCAGC 1 cut(s) 145
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 188
BshFI GGCC 4 cut(s) 8, 65, 357, 926
BsiEI CGRYCG 1 cut(s) 188
BsiHKAI GWGCWC 2 cut(s) 237, 643
BsiSI CCGG 6 cut(s) 39, 120, 130, 137, 188, 800
BslFI GGGAC 5 cut(s) 131, 274, 280, 672, 684
BslI CCNNNNNNNGG 2 cut(s) 136, 364
BsmAI GTCTC 1 cut(s) 584
BsmFI GGGAC 5 cut(s) 131, 274, 280, 672, 684
BsnI GGCC 4 cut(s) 8, 65, 357, 926
Bsp1286I GDGCHC 2 cut(s) 237, 643
Bsp143I GATC 1 cut(s) 385
Bsp19I CCATGG 1 cut(s) 665
BspACI CCGC 3 cut(s) 49, 176, 219
BspANI GGCC 4 cut(s) 8, 65, 357, 926
BspCNI CTCAG 3 cut(s) 307, 337, 928
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 2 cut(s) 37, 672
BspMAI CTGCAG 1 cut(s) 410
BspMI ACCTGC 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 309
BsrFI RCCGGY 2 cut(s) 129, 136
BsrI ACTGG 4 cut(s) 268, 631, 846, 862
BssAI RCCGGY 2 cut(s) 129, 136
BssECI CCNNGG 4 cut(s) 415, 585, 665, 905
BssMI GATC 1 cut(s) 385
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 3 cut(s) 415, 665, 905
Bst2UI CCWGG 2 cut(s) 242, 587
Bst4CI ACNGT 4 cut(s) 22, 55, 103, 199
Bst6I CTCTTC 2 cut(s) 343, 676
BstACI GRCGYC 1 cut(s) 72
BstBAI YACGTR 1 cut(s) 93
BstC8I GCNNGC 3 cut(s) 715, 772, 912
BstDEI CTNAG 5 cut(s) 315, 345, 611, 915, 929
BstDSI CCRYGG 1 cut(s) 665
BstEII GGTNACC 1 cut(s) 41
BstF5I GGATG 4 cut(s) 281, 425, 581, 715
BstFNI CGCG 1 cut(s) 176
BstH2I RGCGCY 1 cut(s) 771
BstHHI GCGC 3 cut(s) 395, 568, 770
BstKTI GATC 1 cut(s) 388
BstMAI GTCTC 1 cut(s) 584
BstMBI GATC 1 cut(s) 385
BstMCI CGRYCG 1 cut(s) 188
BstMWI GCNNNNNNNGC 2 cut(s) 71, 647
BstNI CCWGG 2 cut(s) 242, 587
BstNSI RCATGY 2 cut(s) 717, 728
BstPI GGTNACC 1 cut(s) 41
BstSCI CCNGG 4 cut(s) 118, 240, 585, 799
BstSFI CTRYAG 2 cut(s) 406, 594
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 145
Bsu36I CCTNAGG 2 cut(s) 345, 611
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 4 cut(s) 8, 65, 357, 926
BtgI CCRYGG 1 cut(s) 665
BtrI CACGTC 1 cut(s) 207
BtsCI GGATG 4 cut(s) 281, 425, 581, 715
BtsI GCAGTG 1 cut(s) 538
BtsIMutI CAGTG 2 cut(s) 99, 538
BveI ACCTGC 1 cut(s) 542
Cac8I GCNNGC 3 cut(s) 715, 772, 912
CfoI GCGC 3 cut(s) 395, 568, 770
Cfr10I RCCGGY 2 cut(s) 129, 136
Cfr13I GGNCC 4 cut(s) 355, 671, 797, 925
CseI GACGC 1 cut(s) 80
Csp6I GTAC 3 cut(s) 195, 285, 436
CviAII CATG 5 cut(s) 666, 714, 725, 828, 875
CviQI GTAC 3 cut(s) 195, 285, 436
DdeI CTNAG 5 cut(s) 315, 345, 611, 915, 929
DpnI GATC 1 cut(s) 387
DpnII GATC 1 cut(s) 385
Eam1104I CTCTTC 2 cut(s) 343, 676
EarI CTCTTC 2 cut(s) 343, 676
Eco130I CCWWGG 3 cut(s) 415, 665, 905
Eco47I GGWCC 2 cut(s) 671, 797
Eco47III AGCGCT 1 cut(s) 769
Eco72I CACGTG 1 cut(s) 93
Eco81I CCTNAGG 2 cut(s) 345, 611
Eco91I GGTNACC 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 671
EcoO65I GGTNACC 1 cut(s) 41
EcoRII CCWGG 2 cut(s) 240, 585
EcoT14I CCWWGG 3 cut(s) 415, 665, 905
EcoT22I ATGCAT 2 cut(s) 715, 719
ErhI CCWWGG 3 cut(s) 415, 665, 905
FaeI CATG 5 cut(s) 669, 717, 728, 831, 878
FalI AAGNNNNNCTT 2 cut(s) 754, 786
FaqI GGGAC 5 cut(s) 131, 274, 280, 672, 684
FatI CATG 5 cut(s) 665, 713, 724, 827, 874
FauI CCCGC 1 cut(s) 169
FblI GTMKAC 1 cut(s) 489
Fnu4HI GCNGC 1 cut(s) 134
FokI GGATG 4 cut(s) 288, 432, 568, 722
Fsp4HI GCNGC 1 cut(s) 134
FspBI CTAG 2 cut(s) 249, 834
FspI TGCGCA 1 cut(s) 567
GlaI GCGC 3 cut(s) 394, 567, 769
GluI GCNGC 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 771
HaeIII GGCC 4 cut(s) 8, 65, 357, 926
HapII CCGG 6 cut(s) 39, 120, 130, 137, 188, 800
HgaI GACGC 1 cut(s) 80
HhaI GCGC 3 cut(s) 395, 568, 770
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 5 cut(s) 669, 717, 728, 831, 878
Hin6I GCGC 3 cut(s) 393, 566, 768
HinP1I GCGC 3 cut(s) 393, 566, 768
HinfI GANTC 2 cut(s) 106, 411
HpaII CCGG 6 cut(s) 39, 120, 130, 137, 188, 800
HphI GGTGA 2 cut(s) 53, 265
Hpy166II GTNNAC 1 cut(s) 490
Hpy188I TCNGA 1 cut(s) 918
Hpy188III TCNNGA 2 cut(s) 59, 344
Hpy8I GTNNAC 1 cut(s) 490
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 3 cut(s) 290, 406, 473
HpyCH4III ACNGT 4 cut(s) 22, 55, 103, 199
HpyCH4IV ACGT 2 cut(s) 92, 206
HpyCH4V TGCA 8 cut(s) 87, 408, 442, 543, 695, 713, 717, 947
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 647
HpyF3I CTNAG 5 cut(s) 315, 345, 611, 915, 929
HpySE526I ACGT 2 cut(s) 92, 206
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 5 cut(s) 669, 717, 728, 831, 878
HspAI GCGC 3 cut(s) 393, 566, 768
Kzo9I GATC 1 cut(s) 385
LmnI GCTCC 4 cut(s) 41, 121, 523, 646
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 3 cut(s) 132, 700, 726
MaeI CTAG 2 cut(s) 249, 834
MaeII ACGT 2 cut(s) 92, 206
MaeIII GTNAC 5 cut(s) 24, 41, 376, 823, 878
MalI GATC 1 cut(s) 387
MboI GATC 1 cut(s) 385
MboII GAAGA 2 cut(s) 330, 693
MfeI CAATTG 1 cut(s) 288
MhlI GDGCHC 2 cut(s) 237, 643
MluCI AATT 3 cut(s) 288, 369, 739
MnlI CCTC 9 cut(s) 55, 90, 173, 184, 295, 340, 629, 844, 878
Mph1103I ATGCAT 2 cut(s) 715, 719
MspI CCGG 6 cut(s) 39, 120, 130, 137, 188, 800
MspR9I CCNGG 4 cut(s) 120, 242, 587, 801
MunI CAATTG 1 cut(s) 288
MvaI CCWGG 2 cut(s) 242, 587
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 71, 647
NciI CCSGG 2 cut(s) 120, 801
NcoI CCATGG 1 cut(s) 665
NdeII GATC 1 cut(s) 385
NlaIII CATG 5 cut(s) 669, 717, 728, 831, 878
NlaIV GGNNCC 2 cut(s) 37, 672
NmuCI GTSAC 3 cut(s) 24, 41, 823
NsbI TGCGCA 1 cut(s) 567
NsiI ATGCAT 2 cut(s) 715, 719
NspI RCATGY 2 cut(s) 717, 728
PaeI GCATGC 1 cut(s) 717
PciI ACATGT 1 cut(s) 724
PfeI GAWTC 2 cut(s) 106, 411
PflMI CCANNNNNTGG 1 cut(s) 364
PkrI GCNGC 1 cut(s) 135
PmaCI CACGTG 1 cut(s) 93
PmlI CACGTG 1 cut(s) 93
Ppu21I YACGTR 1 cut(s) 93
PpuMI RGGWCCY 1 cut(s) 671
PscI ACATGT 1 cut(s) 724
Psp5II RGGWCCY 1 cut(s) 671
Psp6I CCWGG 2 cut(s) 240, 585
PspCI CACGTG 1 cut(s) 93
PspEI GGTNACC 1 cut(s) 41
PspGI CCWGG 2 cut(s) 240, 585
PspN4I GGNNCC 2 cut(s) 37, 672
PspPI GGNCC 4 cut(s) 355, 671, 797, 925
PspPPI RGGWCCY 1 cut(s) 671
PstI CTGCAG 1 cut(s) 410
RsaI GTAC 3 cut(s) 196, 286, 437
RsaNI GTAC 3 cut(s) 195, 285, 436
SatI GCNGC 1 cut(s) 134
Sau3AI GATC 1 cut(s) 385
Sau96I GGNCC 4 cut(s) 355, 671, 797, 925
ScrFI CCNGG 4 cut(s) 120, 242, 587, 801
SduI GDGCHC 2 cut(s) 237, 643
SfaNI GCATC 3 cut(s) 132, 700, 726
SfcI CTRYAG 2 cut(s) 406, 594
SinI GGWCC 2 cut(s) 671, 797
SmlI CTYRAG 1 cut(s) 869
SmoI CTYRAG 1 cut(s) 869
SphI GCATGC 1 cut(s) 717
Sse9I AATT 3 cut(s) 288, 369, 739
SsiI CCGC 3 cut(s) 49, 176, 219
SspMI CTAG 2 cut(s) 249, 834
StyD4I CCNGG 4 cut(s) 118, 240, 585, 799
StyI CCWWGG 3 cut(s) 415, 665, 905
TaaI ACNGT 4 cut(s) 22, 55, 103, 199
TaiI ACGT 2 cut(s) 95, 209
TaqI TCGA 3 cut(s) 69, 231, 885
TasI AATT 3 cut(s) 288, 369, 739
TatI WGTACW 2 cut(s) 284, 435
TfiI GAWTC 2 cut(s) 106, 411
TscAI CASTG 2 cut(s) 106, 545
TseFI GTSAC 3 cut(s) 24, 41, 823
TseI GCWGC 1 cut(s) 133
Tsp45I GTSAC 3 cut(s) 24, 41, 823
TspDTI ATGAA 4 cut(s) 298, 484, 547, 774
TspRI CASTG 2 cut(s) 106, 545
Van91I CCANNNNNTGG 1 cut(s) 364
VpaK11BI GGWCC 2 cut(s) 671, 797
XceI RCATGY 2 cut(s) 717, 728
XmiI GTMKAC 1 cut(s) 489
XspI CTAG 2 cut(s) 249, 834
Zsp2I ATGCAT 2 cut(s) 715, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.