FvH4_1g06980

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004453
Physical Location & Seq
Reverse (-)
3688362 .. 3689766
1405 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06980.t1

Sequence Viewer

Length: 948 bp
ATGTCGAAAACGGTGTCACATGACGGCTCCGGTGACTACCGCACCGTACAGGAGGCCATCGACGCCGTTCCTCTCCGAAACAACTCACGCATTGTGATCCGTATACTTCCCGGCATCTACAACCAGCCCGTGTATGTCCCCAAGACCAAGAACCTCATCACACTCACGGCTTGGCTTCCGGAGGACACTGTGCTGACATGGAACAACACCGCCACCAAAATCGATCACCACCAGAATCCTGACATAATCGGAACCGGAACTTTTGCATGCGGGAGCACAATTGTGGAAGGAGAGGACTTCATTGCTGAGAATATCACTTTCGAGAACTCCTCTCCTGAGGGCTCAGGCCAAGCTGTGGCAATGAGAGTGACAGCGGACCGGTGTGCCTTCTATAACTGCAGATTCCTTGGATGGCAAGACACCCTATACTTACATTATGGAAAACACTACTTGAAAGATTGTTACATTGAAGGAAGTGTGGACTTCATTTTTGGTAACAGCACAGCTCTTTTGGAGCATTGCCATATCCACTGCAAATCGGAAGGTTTCATAACTGCACACAAAAGAAACACATGCGAGGAGCCAACAGGGTATGTGTTCTTAAGATGTGTGATCACCGGAAACGGAGGTAGTACTCCGTACGTGTTTCTGGGGCGACCTTGGGGACCTTGTGGAAGAGTGGTGTTTGCGTTTACGTATATGGATGCTTGTGTGAAACATGTAGGGTGGAATAACTGGGATAAAGTAGAGAATGAAGGGAGCGCTTGCTTTAATGAATACAGGTGTTTTGGACCTGGGAGTTGCCCGGAGAAACGAGTGAGATGGGCTAGACAACTGGTGTGTGAGGAAGCAGATCAGTATCTCAGGCATTGTTACATCGATCCAGACCCCCAGAAGTCTTGGCTTGCTGAGAGAACGGCGGCCCTTAGAATACCACATTCTGCATAG

Protein Analysis

316

Amino Acids

35.51

Weight (kDa)

6.17

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 4 - 289 1.8e-65 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 355
AccI GTMKAC 1 cut(s) 103
AccIII TCCGGA 1 cut(s) 178
AciI CCGC 5 cut(s) 40, 210, 270, 374, 920
AclWI GGATC 2 cut(s) 91, 875
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 3 cut(s) 48, 634, 641
AfeI AGCGCT 1 cut(s) 763
AfiI CCNNNNNNNGG 1 cut(s) 355
AflII CTTAAG 1 cut(s) 601
AflIII ACRYGT 2 cut(s) 642, 718
AgeI ACCGGT 1 cut(s) 378
AgsI TTSAA 2 cut(s) 454, 470
AjnI CCWGG 1 cut(s) 793
AleI CACNNNNGTG 1 cut(s) 281
AluBI AGCT 2 cut(s) 353, 506
AluI AGCT 2 cut(s) 353, 506
Alw21I GWGCWC 1 cut(s) 278
AlwI GGATC 2 cut(s) 91, 875
Aor13HI TCCGGA 1 cut(s) 178
Aor51HI AGCGCT 1 cut(s) 763
AoxI GGCC 3 cut(s) 54, 346, 921
ArsI GACNNNNNNTTYG 3 cut(s) 31, 473, 505
AsiGI ACCGGT 1 cut(s) 378
AspLEI GCGC 1 cut(s) 764
AspS9I GGNCC 4 cut(s) 376, 665, 791, 922
AsuC2I CCSGG 2 cut(s) 111, 806
AsuHPI GGTGA 3 cut(s) 44, 218, 607
AvaII GGWCC 3 cut(s) 376, 665, 791
AxyI CCTNAGG 1 cut(s) 336
BanII GRGCYC 1 cut(s) 344
Bbv12I GWGCWC 1 cut(s) 278
BccI CCATC 3 cut(s) 65, 405, 816
BceAI ACGGC 4 cut(s) 40, 50, 183, 933
BciT130I CCWGG 1 cut(s) 795
BclI TGATCA 1 cut(s) 612
BcnI CCSGG 2 cut(s) 111, 806
BfaI CTAG 1 cut(s) 828
BfmI CTRYAG 1 cut(s) 397
BfoI RGCGCY 1 cut(s) 765
BfrI CTTAAG 1 cut(s) 601
BisI GCNGC 1 cut(s) 921
BlsI GCNGC 1 cut(s) 922
BmcAI AGTACT 1 cut(s) 634
Bme1390I CCNGG 3 cut(s) 111, 795, 806
Bme18I GGWCC 3 cut(s) 376, 665, 791
BmgT120I GGNCC 4 cut(s) 376, 665, 791, 922
BmiI GGNNCC 4 cut(s) 28, 253, 582, 666
BmrFI CCNGG 3 cut(s) 111, 795, 806
BmrI ACTGGG 1 cut(s) 745
BmsI GCATC 2 cut(s) 123, 694
BmuI ACTGGG 1 cut(s) 745
BplI GAGNNNNNCTC 2 cut(s) 314, 346
Bpu10I CCTNAGC 1 cut(s) 343
BpuMI CCSGG 2 cut(s) 111, 806
Bsa29I ATCGAT 2 cut(s) 222, 879
BsaAI YACGTR 2 cut(s) 643, 696
BsaBI GATNNNNATC 1 cut(s) 858
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 3 cut(s) 406, 659, 794
BsaWI WCCGGW 5 cut(s) 29, 178, 254, 378, 617
Bsc4I CCNNNNNNNGG 1 cut(s) 355
Bse118I RCCGGY 1 cut(s) 378
Bse1I ACTGG 2 cut(s) 740, 840
Bse21I CCTNAGG 1 cut(s) 336
Bse3DI GCAATG 3 cut(s) 300, 366, 517
Bse8I GATNNNNATC 1 cut(s) 858
BseAI TCCGGA 1 cut(s) 178
BseBI CCWGG 1 cut(s) 795
BseCI ATCGAT 2 cut(s) 222, 879
BseDI CCNNGG 3 cut(s) 406, 659, 794
BseGI GGATG 2 cut(s) 416, 709
BseJI GATNNNNATC 1 cut(s) 858
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMI GCAATG 3 cut(s) 300, 366, 517
BseMII CTCAG 5 cut(s) 297, 327, 357, 877, 900
BseNI ACTGG 2 cut(s) 740, 840
BseRI GAGGAG 2 cut(s) 319, 593
BsgI GTGCAG 1 cut(s) 540
BshFI GGCC 3 cut(s) 56, 348, 923
BshTI ACCGGT 1 cut(s) 378
BshVI ATCGAT 2 cut(s) 222, 879
BsiHKAI GWGCWC 1 cut(s) 278
BsiSI CCGG 7 cut(s) 30, 111, 179, 255, 379, 618, 806
BsiWI CGTACG 1 cut(s) 639
BslFI GGGAC 2 cut(s) 122, 678
BslI CCNNNNNNNGG 1 cut(s) 355
BsmFI GGGAC 2 cut(s) 122, 678
BsnI GGCC 3 cut(s) 56, 348, 923
Bsp1286I GDGCHC 2 cut(s) 278, 344
Bsp13I TCCGGA 1 cut(s) 178
Bsp143I GATC 5 cut(s) 96, 223, 612, 853, 880
BspACI CCGC 5 cut(s) 40, 210, 270, 374, 920
BspANI GGCC 3 cut(s) 56, 348, 923
BspCNI CTCAG 5 cut(s) 298, 328, 356, 876, 901
BspDI ATCGAT 2 cut(s) 222, 879
BspEI TCCGGA 1 cut(s) 178
BspLI GGNNCC 4 cut(s) 28, 253, 582, 666
BspMAI CTGCAG 1 cut(s) 401
BspPI GGATC 2 cut(s) 91, 875
BspTI CTTAAG 1 cut(s) 601
BsrDI GCAATG 3 cut(s) 300, 366, 517
BsrFI RCCGGY 1 cut(s) 378
BsrI ACTGG 2 cut(s) 740, 840
BssAI RCCGGY 1 cut(s) 378
BssECI CCNNGG 3 cut(s) 406, 659, 794
BssMI GATC 5 cut(s) 96, 223, 612, 853, 880
BssNAI GTATAC 1 cut(s) 104
BssNI GRCGYC 1 cut(s) 63
BssT1I CCWWGG 2 cut(s) 406, 659
Bst1107I GTATAC 1 cut(s) 104
Bst2UI CCWGG 1 cut(s) 795
Bst4CI ACNGT 3 cut(s) 13, 46, 190
Bst6I CTCTTC 1 cut(s) 670
BstACI GRCGYC 1 cut(s) 63
BstAFI CTTAAG 1 cut(s) 601
BstBAI YACGTR 2 cut(s) 643, 696
BstC8I GCNNGC 3 cut(s) 268, 766, 906
BstDEI CTNAG 6 cut(s) 306, 336, 343, 863, 909, 926
BstF5I GGATG 2 cut(s) 416, 709
BstH2I RGCGCY 1 cut(s) 765
BstHHI GCGC 1 cut(s) 764
BstKTI GATC 5 cut(s) 99, 226, 615, 856, 883
BstMBI GATC 5 cut(s) 96, 223, 612, 853, 880
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 795
BstNSI RCATGY 3 cut(s) 270, 576, 722
BstSCI CCNGG 3 cut(s) 109, 793, 804
BstSFI CTRYAG 1 cut(s) 397
BstSNI TACGTA 1 cut(s) 696
BstZ17I GTATAC 1 cut(s) 104
Bsu15I ATCGAT 2 cut(s) 222, 879
Bsu36I CCTNAGG 1 cut(s) 336
BsuRI GGCC 3 cut(s) 56, 348, 923
BsuTUI ATCGAT 2 cut(s) 222, 879
BtsCI GGATG 2 cut(s) 416, 709
BtsI GCAGTG 1 cut(s) 529
BtsIMutI CAGTG 2 cut(s) 186, 529
Cac8I GCNNGC 3 cut(s) 268, 766, 906
CfoI GCGC 1 cut(s) 764
Cfr10I RCCGGY 1 cut(s) 378
Cfr13I GGNCC 4 cut(s) 376, 665, 791, 922
ClaI ATCGAT 2 cut(s) 222, 879
CpoI CGGWCCG 1 cut(s) 376
CseI GACGC 1 cut(s) 71
Csp6I GTAC 3 cut(s) 47, 633, 640
CspAI ACCGGT 1 cut(s) 378
CspI CGGWCCG 1 cut(s) 376
CviAII CATG 5 cut(s) 20, 198, 267, 573, 719
CviQI GTAC 3 cut(s) 47, 633, 640
DdeI CTNAG 6 cut(s) 306, 336, 343, 863, 909, 926
DpnI GATC 5 cut(s) 98, 225, 614, 855, 882
DpnII GATC 5 cut(s) 96, 223, 612, 853, 880
Eam1104I CTCTTC 1 cut(s) 670
EarI CTCTTC 1 cut(s) 670
Eco105I TACGTA 1 cut(s) 696
Eco130I CCWWGG 2 cut(s) 406, 659
Eco24I GRGCYC 1 cut(s) 344
Eco47I GGWCC 3 cut(s) 376, 665, 791
Eco47III AGCGCT 1 cut(s) 763
Eco81I CCTNAGG 1 cut(s) 336
EcoO109I RGGNCCY 1 cut(s) 665
EcoRII CCWGG 1 cut(s) 793
EcoT14I CCWWGG 2 cut(s) 406, 659
EcoT38I GRGCYC 1 cut(s) 344
ErhI CCWWGG 2 cut(s) 406, 659
FaeI CATG 5 cut(s) 23, 201, 270, 576, 722
FaqI GGGAC 2 cut(s) 122, 678
FatI CATG 5 cut(s) 19, 197, 266, 572, 718
FauI CCCGC 1 cut(s) 263
FbaI TGATCA 1 cut(s) 612
FblI GTMKAC 1 cut(s) 103
Fnu4HI GCNGC 1 cut(s) 921
FokI GGATG 2 cut(s) 423, 716
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 1 cut(s) 921
FspBI CTAG 1 cut(s) 828
GlaI GCGC 1 cut(s) 763
GluI GCNGC 1 cut(s) 921
HaeII RGCGCY 1 cut(s) 765
HaeIII GGCC 3 cut(s) 56, 348, 923
HapII CCGG 7 cut(s) 30, 111, 179, 255, 379, 618, 806
HgaI GACGC 1 cut(s) 71
HhaI GCGC 1 cut(s) 764
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 5 cut(s) 23, 201, 270, 576, 722
Hin6I GCGC 1 cut(s) 762
HinP1I GCGC 1 cut(s) 762
HinfI GANTC 2 cut(s) 235, 402
HpaII CCGG 7 cut(s) 30, 111, 179, 255, 379, 618, 806
HphI GGTGA 3 cut(s) 44, 218, 607
Hpy166II GTNNAC 3 cut(s) 104, 481, 693
Hpy188I TCNGA 3 cut(s) 77, 251, 541
Hpy188III TCNNGA 5 cut(s) 179, 239, 322, 335, 884
Hpy8I GTNNAC 3 cut(s) 104, 481, 693
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 5 cut(s) 281, 397, 464, 536, 749
HpyCH4III ACNGT 3 cut(s) 13, 46, 190
HpyCH4IV ACGT 2 cut(s) 642, 695
HpyCH4V TGCA 5 cut(s) 266, 399, 534, 557, 944
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 6 cut(s) 306, 336, 343, 863, 909, 926
HpySE526I ACGT 2 cut(s) 642, 695
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 5 cut(s) 23, 201, 270, 576, 722
HspAI GCGC 1 cut(s) 762
Kpn2I TCCGGA 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 612
Kzo9I GATC 5 cut(s) 96, 223, 612, 853, 880
LmnI GCTCC 5 cut(s) 32, 273, 514, 580, 759
LweI GCATC 2 cut(s) 123, 694
MaeI CTAG 1 cut(s) 828
MaeII ACGT 2 cut(s) 642, 695
MaeIII GTNAC 6 cut(s) 15, 32, 367, 461, 494, 872
MalI GATC 5 cut(s) 98, 225, 614, 855, 882
MboI GATC 5 cut(s) 96, 223, 612, 853, 880
MboII GAAGA 1 cut(s) 687
MfeI CAATTG 1 cut(s) 279
MhlI GDGCHC 2 cut(s) 278, 344
MluCI AATT 1 cut(s) 279
MroI TCCGGA 1 cut(s) 178
MseI TTAA 2 cut(s) 602, 771
MslI CAYNNNNRTG 1 cut(s) 281
MspA1I CMGCKG 1 cut(s) 374
MspCI CTTAAG 1 cut(s) 601
MspI CCGG 7 cut(s) 30, 111, 179, 255, 379, 618, 806
MspR9I CCNGG 3 cut(s) 111, 795, 806
MunI CAATTG 1 cut(s) 279
MvaI CCWGG 1 cut(s) 795
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 2 cut(s) 111, 806
NdeII GATC 5 cut(s) 96, 223, 612, 853, 880
NlaIII CATG 5 cut(s) 23, 201, 270, 576, 722
NlaIV GGNNCC 4 cut(s) 28, 253, 582, 666
NmuCI GTSAC 3 cut(s) 15, 32, 367
NspI RCATGY 3 cut(s) 270, 576, 722
OliI CACNNNNGTG 1 cut(s) 281
PaeI GCATGC 1 cut(s) 270
PciI ACATGT 1 cut(s) 718
PfeI GAWTC 2 cut(s) 235, 402
Pfl23II CGTACG 1 cut(s) 639
PflMI CCANNNNNTGG 1 cut(s) 355
PinAI ACCGGT 1 cut(s) 378
PkrI GCNGC 1 cut(s) 922
Ppu21I YACGTR 2 cut(s) 643, 696
PpuMI RGGWCCY 1 cut(s) 665
PscI ACATGT 1 cut(s) 718
Psp5II RGGWCCY 1 cut(s) 665
Psp6I CCWGG 1 cut(s) 793
PspGI CCWGG 1 cut(s) 793
PspLI CGTACG 1 cut(s) 639
PspN4I GGNNCC 4 cut(s) 28, 253, 582, 666
PspPI GGNCC 4 cut(s) 376, 665, 791, 922
PspPPI RGGWCCY 1 cut(s) 665
PstI CTGCAG 1 cut(s) 401
RsaI GTAC 3 cut(s) 48, 634, 641
RsaNI GTAC 3 cut(s) 47, 633, 640
RseI CAYNNNNRTG 1 cut(s) 281
Rsr2I CGGWCCG 1 cut(s) 376
RsrII CGGWCCG 1 cut(s) 376
SaqAI TTAA 2 cut(s) 602, 771
SatI GCNGC 1 cut(s) 921
Sau3AI GATC 5 cut(s) 96, 223, 612, 853, 880
Sau96I GGNCC 4 cut(s) 376, 665, 791, 922
ScaI AGTACT 1 cut(s) 634
ScrFI CCNGG 3 cut(s) 111, 795, 806
SduI GDGCHC 2 cut(s) 278, 344
SfaNI GCATC 2 cut(s) 123, 694
SfcI CTRYAG 1 cut(s) 397
SinI GGWCC 3 cut(s) 376, 665, 791
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 601
SmoI CTYRAG 1 cut(s) 601
SnaBI TACGTA 1 cut(s) 696
SphI GCATGC 1 cut(s) 270
Sse9I AATT 1 cut(s) 279
SsiI CCGC 5 cut(s) 40, 210, 270, 374, 920
SspMI CTAG 1 cut(s) 828
StyD4I CCNGG 3 cut(s) 109, 793, 804
StyI CCWWGG 2 cut(s) 406, 659
TaaI ACNGT 3 cut(s) 13, 46, 190
TaiI ACGT 2 cut(s) 645, 698
TaqI TCGA 5 cut(s) 5, 60, 222, 321, 879
TasI AATT 1 cut(s) 279
TatI WGTACW 1 cut(s) 632
TauI GCSGC 1 cut(s) 923
TfiI GAWTC 2 cut(s) 235, 402
Tru1I TTAA 2 cut(s) 602, 771
Tru9I TTAA 2 cut(s) 602, 771
TscAI CASTG 2 cut(s) 193, 536
TseFI GTSAC 3 cut(s) 15, 32, 367
Tsp45I GTSAC 3 cut(s) 15, 32, 367
TspDTI ATGAA 5 cut(s) 289, 475, 538, 768, 789
TspGWI ACGGA 3 cut(s) 89, 627, 639
TspRI CASTG 2 cut(s) 193, 536
Van91I CCANNNNNTGG 1 cut(s) 355
Vha464I CTTAAG 1 cut(s) 601
VpaK11BI GGWCC 3 cut(s) 376, 665, 791
XceI RCATGY 3 cut(s) 270, 576, 722
XmiI GTMKAC 1 cut(s) 103
XspI CTAG 1 cut(s) 828
ZrmI AGTACT 1 cut(s) 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.