RchiOBHm_Chr2g0092931

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004453
Physical Location & Seq
Reverse (-)
6353238 .. 6354726
1489 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46800

Sequence Viewer

Length: 1071 bp
ATGAGTAGGGTACGTACCACGATCCTCATCGGGTTTCCCATAAGAAGATCAGATCCACAACGAGACTCCATTTCCTGCTGTCTAATTCTCAACAAAATAATTCTAGGCAAACGCCTTGAAATGTCCGACTGTGTGAAAACGGTGTCACAGGACGGCTCCGGTGACTACCGCACCGTACAGGAGGCCATCGACGCCGTGCCTCTCAAAAACACGTGTCGCATTGTGATTCGTATATCTCCCGGCATCTACCAGCAGCCGGTGTATGTCCCCAAGACCAAGAACCTCATCACACTGACTGGTTTGCTTCCGGAGGAGACTGTGCTGACGTGGAACAACACCGCCACCAAAATCGAGCACCACCAGAATTCTGAGGTGATTGGGACTGGGACTTTTGCATGTGGGAGTACAATACTGGAAGGAGAGGACTTCATTGCTGAGAATATCACTTTTGAGAACTCTTCTCCTGAGGGTTCAGGCCAAGCTGTGGCAATGAGAGTAACAGCGGATAGGTGTGCCTTCTATAACTGCAGATTCCTTGGATGGCAGGATACCCTATACTTACATCATGGAAAGCAGTATTTGAAAGATTGTTATATTGAAGGAAGTGTAGACTTCATTTTCGGCAATAGCACAGCTCTTTTGGAGCATTGTCATATCCACTGCAAGTCGGAAGGTTTCATAACCGCGCACAAAAGAAATTCATGCGAGGAGGCAACAGGATATGTGTTCCTAAGATGTGTAATCACTGGAAATGGAGGTACTCCATACGCATTTCTGGGACGACCATGGGGACCTTGTGGAAGAGTGGTCTTTGCATTTACATATATGGATGCATGCATCAAACATGTAGGCTGGAATAATTGGGATAAAACAGAGAATGAAGGAAGCGCTTGCTTTAACGAATACAGGTGTTTCGGACCGGGTAGTTGCCCAGAAAAACGAGTGACATGGGCTAGACAACTGGGATGTGAGGAAGCAGACCATTTCCTCAATCATTATTACATCGACCCAGACCCGCAAAGGCCTTGGCTTGCTCAGAGAATGGCCCTAAGAATACCACATTCTGCATAG

Protein Analysis

356

Amino Acids

40.06

Weight (kDa)

6.63

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 47 - 336 7.9e-69 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 484
AccI GTMKAC 1 cut(s) 609
AccII CGCG 1 cut(s) 686
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 5 cut(s) 169, 339, 503, 684, 1016
AclWI GGATC 2 cut(s) 16, 47
AcsI RAATTY 2 cut(s) 364, 697
AcvI CACGTG 1 cut(s) 213
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 5 cut(s) 12, 16, 177, 406, 760
AfeI AGCGCT 1 cut(s) 889
AfiI CCNNNNNNNGG 2 cut(s) 256, 484
AflIII ACRYGT 3 cut(s) 210, 212, 844
AgsI TTSAA 3 cut(s) 119, 583, 599
AjiI CACGTC 1 cut(s) 327
AluBI AGCT 2 cut(s) 482, 635
AluI AGCT 2 cut(s) 482, 635
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 2 cut(s) 57, 308
AlwI GGATC 2 cut(s) 16, 47
Aor13HI TCCGGA 1 cut(s) 307
Aor51HI AGCGCT 1 cut(s) 889
AoxI GGCC 4 cut(s) 183, 475, 1022, 1044
ApeKI GCWGC 1 cut(s) 253
ApoI RAATTY 2 cut(s) 364, 697
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
AspLEI GCGC 2 cut(s) 688, 890
AspS9I GGNCC 3 cut(s) 791, 917, 1045
AsuC2I CCSGG 2 cut(s) 240, 921
AsuHPI GGTGA 2 cut(s) 173, 385
AvaII GGWCC 2 cut(s) 791, 917
AxyI CCTNAGG 1 cut(s) 465
BbrPI CACGTG 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 357
BbvI GCAGC 1 cut(s) 265
BccI CCATC 2 cut(s) 194, 534
BceAI ACGGC 2 cut(s) 169, 179
BciVI GTATCC 1 cut(s) 541
BcnI CCSGG 2 cut(s) 240, 921
BcoDI GTCTC 2 cut(s) 57, 308
BfaI CTAG 2 cut(s) 104, 954
BfmI CTRYAG 1 cut(s) 526
BfoI RGCGCY 1 cut(s) 891
BfuI GTATCC 1 cut(s) 541
BisI GCNGC 1 cut(s) 254
BlsI GCNGC 1 cut(s) 255
Bme1390I CCNGG 2 cut(s) 240, 921
Bme18I GGWCC 2 cut(s) 791, 917
BmgBI CACGTC 1 cut(s) 327
BmgT120I GGNCC 3 cut(s) 791, 917, 1045
BmiI GGNNCC 2 cut(s) 157, 792
BmrFI CCNGG 2 cut(s) 240, 921
BmrI ACTGGG 2 cut(s) 393, 971
BmsI GCATC 3 cut(s) 252, 820, 846
BmuI ACTGGG 2 cut(s) 393, 971
BpuMI CCSGG 2 cut(s) 240, 921
BsaAI YACGTR 2 cut(s) 14, 213
BsaBI GATNNNNATC 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 3 cut(s) 535, 785, 1025
BsaWI WCCGGW 2 cut(s) 158, 307
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 484
Bse118I RCCGGY 1 cut(s) 256
Bse1I ACTGG 5 cut(s) 301, 388, 417, 751, 966
Bse21I CCTNAGG 1 cut(s) 465
Bse3DI GCAATG 2 cut(s) 429, 495
Bse8I GATNNNNATC 1 cut(s) 26
BseAI TCCGGA 1 cut(s) 307
BseDI CCNNGG 3 cut(s) 535, 785, 1025
BseGI GGATG 3 cut(s) 545, 835, 971
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 2 cut(s) 256, 484
BseMI GCAATG 2 cut(s) 429, 495
BseMII CTCAG 4 cut(s) 360, 426, 456, 1049
BseNI ACTGG 5 cut(s) 301, 388, 417, 751, 966
BseRI GAGGAG 2 cut(s) 326, 722
BseXI GCAGC 1 cut(s) 265
Bsh1236I CGCG 1 cut(s) 686
BshFI GGCC 4 cut(s) 185, 477, 1024, 1046
BsiHKAI GWGCWC 1 cut(s) 357
BsiSI CCGG 5 cut(s) 159, 240, 257, 308, 920
BslFI GGGAC 5 cut(s) 251, 394, 400, 792, 804
BslI CCNNNNNNNGG 2 cut(s) 256, 484
BsmAI GTCTC 2 cut(s) 57, 308
BsmFI GGGAC 5 cut(s) 251, 394, 400, 792, 804
BsnI GGCC 4 cut(s) 185, 477, 1024, 1046
Bsp1286I GDGCHC 1 cut(s) 357
Bsp13I TCCGGA 1 cut(s) 307
Bsp143I GATC 3 cut(s) 21, 47, 52
Bsp19I CCATGG 1 cut(s) 785
BspACI CCGC 5 cut(s) 169, 339, 503, 684, 1016
BspANI GGCC 4 cut(s) 185, 477, 1024, 1046
BspCNI CTCAG 4 cut(s) 361, 427, 457, 1048
BspEI TCCGGA 1 cut(s) 307
BspFNI CGCG 1 cut(s) 686
BspLI GGNNCC 2 cut(s) 157, 792
BspMAI CTGCAG 1 cut(s) 530
BspPI GGATC 2 cut(s) 16, 47
BsrDI GCAATG 2 cut(s) 429, 495
BsrFI RCCGGY 1 cut(s) 256
BsrI ACTGG 5 cut(s) 301, 388, 417, 751, 966
BssAI RCCGGY 1 cut(s) 256
BssECI CCNNGG 3 cut(s) 535, 785, 1025
BssMI GATC 3 cut(s) 21, 47, 52
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 3 cut(s) 535, 785, 1025
Bst4CI ACNGT 4 cut(s) 131, 142, 175, 319
Bst6I CTCTTC 2 cut(s) 463, 796
BstACI GRCGYC 1 cut(s) 192
BstBAI YACGTR 2 cut(s) 14, 213
BstC8I GCNNGC 3 cut(s) 835, 892, 1032
BstDEI CTNAG 6 cut(s) 369, 435, 465, 731, 1035, 1049
BstDSI CCRYGG 1 cut(s) 785
BstF5I GGATG 3 cut(s) 545, 835, 971
BstFNI CGCG 1 cut(s) 686
BstH2I RGCGCY 1 cut(s) 891
BstHHI GCGC 2 cut(s) 688, 890
BstKTI GATC 3 cut(s) 24, 50, 55
BstMAI GTCTC 2 cut(s) 57, 308
BstMBI GATC 3 cut(s) 21, 47, 52
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstNSI RCATGY 3 cut(s) 399, 837, 848
BstSCI CCNGG 2 cut(s) 238, 919
BstSFI CTRYAG 1 cut(s) 526
BstSNI TACGTA 1 cut(s) 14
BstUI CGCG 1 cut(s) 686
BstV1I GCAGC 1 cut(s) 265
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
Bsu36I CCTNAGG 1 cut(s) 465
BsuI GTATCC 1 cut(s) 541
BsuRI GGCC 4 cut(s) 185, 477, 1024, 1046
BtgI CCRYGG 1 cut(s) 785
BtrI CACGTC 1 cut(s) 327
BtsCI GGATG 3 cut(s) 545, 835, 971
BtsI GCAGTG 1 cut(s) 658
BtsIMutI CAGTG 3 cut(s) 290, 658, 744
Cac8I GCNNGC 3 cut(s) 835, 892, 1032
CfoI GCGC 2 cut(s) 688, 890
Cfr10I RCCGGY 1 cut(s) 256
Cfr13I GGNCC 3 cut(s) 791, 917, 1045
CpoI CGGWCCG 1 cut(s) 917
CseI GACGC 1 cut(s) 200
Csp6I GTAC 5 cut(s) 11, 15, 176, 405, 759
CspI CGGWCCG 1 cut(s) 917
CviAII CATG 7 cut(s) 396, 566, 702, 786, 834, 845, 948
CviQI GTAC 5 cut(s) 11, 15, 176, 405, 759
DdeI CTNAG 6 cut(s) 369, 435, 465, 731, 1035, 1049
DpnI GATC 3 cut(s) 23, 49, 54
DpnII GATC 3 cut(s) 21, 47, 52
Eam1104I CTCTTC 2 cut(s) 463, 796
EarI CTCTTC 2 cut(s) 463, 796
Eco105I TACGTA 1 cut(s) 14
Eco130I CCWWGG 3 cut(s) 535, 785, 1025
Eco147I AGGCCT 1 cut(s) 1024
Eco47I GGWCC 2 cut(s) 791, 917
Eco47III AGCGCT 1 cut(s) 889
Eco72I CACGTG 1 cut(s) 213
Eco81I CCTNAGG 1 cut(s) 465
EcoO109I RGGNCCY 1 cut(s) 791
EcoRI GAATTC 1 cut(s) 364
EcoT14I CCWWGG 3 cut(s) 535, 785, 1025
EcoT22I ATGCAT 2 cut(s) 835, 839
ErhI CCWWGG 3 cut(s) 535, 785, 1025
FaeI CATG 7 cut(s) 399, 569, 705, 789, 837, 848, 951
FaqI GGGAC 5 cut(s) 251, 394, 400, 792, 804
FatI CATG 7 cut(s) 395, 565, 701, 785, 833, 844, 947
FauI CCCGC 1 cut(s) 1023
FblI GTMKAC 1 cut(s) 609
Fnu4HI GCNGC 1 cut(s) 254
FokI GGATG 3 cut(s) 552, 842, 978
Fsp4HI GCNGC 1 cut(s) 254
FspBI CTAG 2 cut(s) 104, 954
GlaI GCGC 2 cut(s) 687, 889
GluI GCNGC 1 cut(s) 254
HaeII RGCGCY 1 cut(s) 891
HaeIII GGCC 4 cut(s) 185, 477, 1024, 1046
HapII CCGG 5 cut(s) 159, 240, 257, 308, 920
HgaI GACGC 1 cut(s) 200
HhaI GCGC 2 cut(s) 688, 890
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 7 cut(s) 399, 569, 705, 789, 837, 848, 951
Hin6I GCGC 2 cut(s) 686, 888
HinP1I GCGC 2 cut(s) 686, 888
HinfI GANTC 3 cut(s) 65, 226, 531
HpaII CCGG 5 cut(s) 159, 240, 257, 308, 920
HphI GGTGA 2 cut(s) 173, 385
Hpy166II GTNNAC 1 cut(s) 610
Hpy188I TCNGA 6 cut(s) 52, 127, 370, 670, 917, 1038
Hpy188III TCNNGA 2 cut(s) 308, 464
Hpy8I GTNNAC 1 cut(s) 610
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 5 cut(s) 410, 526, 593, 665, 875
HpyCH4III ACNGT 4 cut(s) 131, 142, 175, 319
HpyCH4IV ACGT 3 cut(s) 13, 212, 326
HpyCH4V TGCA 7 cut(s) 395, 528, 663, 815, 833, 837, 1067
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpyF3I CTNAG 6 cut(s) 369, 435, 465, 731, 1035, 1049
HpySE526I ACGT 3 cut(s) 13, 212, 326
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 7 cut(s) 399, 569, 705, 789, 837, 848, 951
HspAI GCGC 2 cut(s) 686, 888
Kpn2I TCCGGA 1 cut(s) 307
Kzo9I GATC 3 cut(s) 21, 47, 52
LmnI GCTCC 2 cut(s) 161, 643
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 3 cut(s) 252, 820, 846
MaeI CTAG 2 cut(s) 104, 954
MaeII ACGT 3 cut(s) 13, 212, 326
MaeIII GTNAC 4 cut(s) 144, 161, 496, 943
MalI GATC 3 cut(s) 23, 49, 54
MboI GATC 3 cut(s) 21, 47, 52
MboII GAAGA 3 cut(s) 57, 450, 813
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 357
MluCI AATT 5 cut(s) 84, 99, 364, 697, 859
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 2 cut(s) 150, 648
Mph1103I ATGCAT 2 cut(s) 835, 839
MroI TCCGGA 1 cut(s) 307
MseI TTAA 1 cut(s) 897
MspA1I CMGCKG 1 cut(s) 503
MspI CCGG 5 cut(s) 159, 240, 257, 308, 920
MspR9I CCNGG 2 cut(s) 240, 921
MvnI CGCG 1 cut(s) 686
MwoI GCNNNNNNNGC 1 cut(s) 191
NciI CCSGG 2 cut(s) 240, 921
NcoI CCATGG 1 cut(s) 785
NdeII GATC 3 cut(s) 21, 47, 52
NlaIII CATG 7 cut(s) 399, 569, 705, 789, 837, 848, 951
NlaIV GGNNCC 2 cut(s) 157, 792
NmuCI GTSAC 3 cut(s) 144, 161, 943
NsiI ATGCAT 2 cut(s) 835, 839
NspI RCATGY 3 cut(s) 399, 837, 848
PaeI GCATGC 1 cut(s) 837
PceI AGGCCT 1 cut(s) 1024
PciI ACATGT 1 cut(s) 844
PfeI GAWTC 2 cut(s) 226, 531
PflMI CCANNNNNTGG 1 cut(s) 484
PkrI GCNGC 1 cut(s) 255
PleI GAGTC 1 cut(s) 59
PmaCI CACGTG 1 cut(s) 213
PmlI CACGTG 1 cut(s) 213
PpsI GAGTC 1 cut(s) 59
Ppu21I YACGTR 2 cut(s) 14, 213
PpuMI RGGWCCY 1 cut(s) 791
PscI ACATGT 1 cut(s) 844
Psp5II RGGWCCY 1 cut(s) 791
PspCI CACGTG 1 cut(s) 213
PspN4I GGNNCC 2 cut(s) 157, 792
PspPI GGNCC 3 cut(s) 791, 917, 1045
PspPPI RGGWCCY 1 cut(s) 791
PstI CTGCAG 1 cut(s) 530
PsuI RGATCY 1 cut(s) 52
RsaI GTAC 5 cut(s) 12, 16, 177, 406, 760
RsaNI GTAC 5 cut(s) 11, 15, 176, 405, 759
Rsr2I CGGWCCG 1 cut(s) 917
RsrII CGGWCCG 1 cut(s) 917
SaqAI TTAA 1 cut(s) 897
SatI GCNGC 1 cut(s) 254
Sau3AI GATC 3 cut(s) 21, 47, 52
Sau96I GGNCC 3 cut(s) 791, 917, 1045
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 2 cut(s) 240, 921
SduI GDGCHC 1 cut(s) 357
SfaNI GCATC 3 cut(s) 252, 820, 846
SfcI CTRYAG 1 cut(s) 526
SinI GGWCC 2 cut(s) 791, 917
SnaBI TACGTA 1 cut(s) 14
SphI GCATGC 1 cut(s) 837
Sse9I AATT 5 cut(s) 84, 99, 364, 697, 859
SseBI AGGCCT 1 cut(s) 1024
SsiI CCGC 5 cut(s) 169, 339, 503, 684, 1016
SspMI CTAG 2 cut(s) 104, 954
StuI AGGCCT 1 cut(s) 1024
StyD4I CCNGG 2 cut(s) 238, 919
StyI CCWWGG 3 cut(s) 535, 785, 1025
TaaI ACNGT 4 cut(s) 131, 142, 175, 319
TaiI ACGT 3 cut(s) 16, 215, 329
TaqI TCGA 3 cut(s) 189, 351, 1005
TasI AATT 5 cut(s) 84, 99, 364, 697, 859
TatI WGTACW 1 cut(s) 404
TfiI GAWTC 2 cut(s) 226, 531
Tru1I TTAA 1 cut(s) 897
Tru9I TTAA 1 cut(s) 897
TscAI CASTG 3 cut(s) 297, 665, 751
TseFI GTSAC 3 cut(s) 144, 161, 943
TseI GCWGC 1 cut(s) 253
Tsp45I GTSAC 3 cut(s) 144, 161, 943
TspDTI ATGAA 5 cut(s) 418, 604, 667, 690, 894
TspRI CASTG 3 cut(s) 297, 665, 751
Van91I CCANNNNNTGG 1 cut(s) 484
VpaK11BI GGWCC 2 cut(s) 791, 917
XapI RAATTY 2 cut(s) 364, 697
XceI RCATGY 3 cut(s) 399, 837, 848
XmiI GTMKAC 1 cut(s) 609
XspI CTAG 2 cut(s) 104, 954
Zsp2I ATGCAT 2 cut(s) 835, 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.