FvH4_6g48010

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004453
Physical Location & Seq
Reverse (-)
36247833 .. 36251060
3228 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g48010.t1

Sequence Viewer

Length: 954 bp
ATGTCGGCCTATGTGTCAATAACTGTGTCACAGGACGGCTCCGGTGACTATCTCACCGTACAGGAGGCCATCGACGCCGTGCCTCTATGCAACACGTGTCGCACTGTGATTCGTATCAGTCCCGGCACCTACCGGCAGCCGGTGTACGTCCCCAAGACCAAGAACCTCATCACACTCGCGGGTCTGCGCCCGGAGGAGACTGTGCTGACGTGGAACAACACCGCCACCAAAATCGAGCACCACCAGGCTTCTAGGGTGATTGGGACTGGGACTTTTGGATGTGGGAGTACAATTGTGGAAGGAGAGGACTTCATTGCTGAGAATATCACATTTGAGAATTCTTCTCCTGAGGGTTCAGGCCAAGCTGTGGCAATTAGAGTAACAGCGGATCGGTGTGCCTTTTATAACTGCAGATTCCTTGGATGGCAGGATACACTGTACTTGCATTATGGAAAGCAGTATTTGAAAGATTGTTATATTGAAGGAAGTGTAGACTTTATTTTTGGTAATAGCACAGCTCTTTTGGAGCATTGTCATATCCACTGCAAGTCAGCAGGTTTCATAACTGCGCAAAGCAGGAAATCATCCCAGGAGACAACAGGATATGTGTTCCTAAGATGTGTAATAACTGGAAATGGAGGTGCTCCATACACATTACTGGGACGACCATGGGGACCTTTTGGAAGAGTGGTCTTTGCATTTACATATATGGATGCATGCATCAAACATGTAGGCTGGCATAATTGGGGCAAAACAGAGAATGAAAGAAGTGCTTGCTTTTATGAATACAGGTGTTTTGGACCGGGTAGTTGCCCATCAAATCGAGTGACATGGGCTAGAGAACTGGTAGATGAGGAAGCAGACCAGTTCCTCCAGCATTCTTACATCGACCCAGACCCCCAAAGGCCTTGGCTTGCTCAGAGAATGGCCCTAAGAATACCATATTCTGCATAG

Protein Analysis

318

Amino Acids

35.46

Weight (kDa)

6.31

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 7 - 297 1.9e-70 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 405
Acc16I TGCGCA 1 cut(s) 570
Acc36I ACCTGC 1 cut(s) 545
AccB1I GGYRCC 1 cut(s) 125
AccB7I CCANNNNNTGG 1 cut(s) 367
AccI GTMKAC 1 cut(s) 492
AccII CGCG 1 cut(s) 179
AciI CCGC 3 cut(s) 179, 222, 386
AclWI GGATC 1 cut(s) 396
AcsI RAATTY 1 cut(s) 337
AcvI CACGTG 1 cut(s) 96
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 4 cut(s) 60, 146, 289, 440
AfiI CCNNNNNNNGG 2 cut(s) 139, 367
AflIII ACRYGT 3 cut(s) 93, 95, 727
AgsI TTSAA 2 cut(s) 466, 482
AjiI CACGTC 1 cut(s) 210
AjnI CCWGG 2 cut(s) 243, 588
AluBI AGCT 2 cut(s) 365, 518
AluI AGCT 2 cut(s) 365, 518
Alw21I GWGCWC 2 cut(s) 240, 646
Alw26I GTCTC 2 cut(s) 191, 587
AlwI GGATC 1 cut(s) 396
AoxI GGCC 5 cut(s) 6, 66, 358, 905, 927
ApeKI GCWGC 1 cut(s) 136
ApoI RAATTY 1 cut(s) 337
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AspLEI GCGC 2 cut(s) 189, 571
AspS9I GGNCC 3 cut(s) 674, 800, 928
AsuC2I CCSGG 3 cut(s) 123, 191, 804
AsuHPI GGTGA 3 cut(s) 46, 56, 268
AvaII GGWCC 2 cut(s) 674, 800
AxyI CCTNAGG 1 cut(s) 348
BanI GGYRCC 1 cut(s) 125
BbrPI CACGTG 1 cut(s) 96
Bbv12I GWGCWC 2 cut(s) 240, 646
BbvI GCAGC 1 cut(s) 148
BccI CCATC 3 cut(s) 77, 417, 823
BceAI ACGGC 2 cut(s) 52, 62
BciT130I CCWGG 2 cut(s) 245, 590
BciVI GTATCC 1 cut(s) 424
BcnI CCSGG 3 cut(s) 123, 191, 804
BcoDI GTCTC 2 cut(s) 191, 587
BfaI CTAG 2 cut(s) 252, 837
BfmI CTRYAG 1 cut(s) 409
BfuAI ACCTGC 1 cut(s) 545
BfuI GTATCC 1 cut(s) 424
BisI GCNGC 1 cut(s) 137
BlsI GCNGC 1 cut(s) 138
Bme1390I CCNGG 5 cut(s) 123, 191, 245, 590, 804
Bme18I GGWCC 2 cut(s) 674, 800
BmgBI CACGTC 1 cut(s) 210
BmgT120I GGNCC 3 cut(s) 674, 800, 928
BmiI GGNNCC 3 cut(s) 40, 127, 675
BmrFI CCNGG 5 cut(s) 123, 191, 245, 590, 804
BmrI ACTGGG 2 cut(s) 276, 668
BmsI GCATC 2 cut(s) 703, 729
BmuI ACTGGG 2 cut(s) 276, 668
BpmI CTGGAG 1 cut(s) 857
BpuMI CCSGG 3 cut(s) 123, 191, 804
BsaAI YACGTR 1 cut(s) 96
BsaBI GATNNNNATC 1 cut(s) 113
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 4 cut(s) 418, 588, 668, 908
BsaWI WCCGGW 1 cut(s) 41
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 367
Bse118I RCCGGY 2 cut(s) 132, 139
Bse1I ACTGG 5 cut(s) 271, 634, 663, 849, 865
Bse21I CCTNAGG 1 cut(s) 348
Bse3DI GCAATG 1 cut(s) 312
Bse8I GATNNNNATC 1 cut(s) 113
BseBI CCWGG 2 cut(s) 245, 590
BseDI CCNNGG 4 cut(s) 418, 588, 668, 908
BseGI GGATG 4 cut(s) 284, 428, 584, 718
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 2 cut(s) 139, 367
BseMI GCAATG 1 cut(s) 312
BseMII CTCAG 3 cut(s) 309, 339, 932
BseNI ACTGG 5 cut(s) 271, 634, 663, 849, 865
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 1 cut(s) 148
Bsh1236I CGCG 1 cut(s) 179
BshFI GGCC 5 cut(s) 8, 68, 360, 907, 929
BshNI GGYRCC 1 cut(s) 125
BsiHKAI GWGCWC 2 cut(s) 240, 646
BsiSI CCGG 6 cut(s) 42, 123, 133, 140, 191, 803
BslFI GGGAC 6 cut(s) 105, 134, 277, 283, 675, 687
BslI CCNNNNNNNGG 2 cut(s) 139, 367
BsmAI GTCTC 2 cut(s) 191, 587
BsmFI GGGAC 6 cut(s) 105, 134, 277, 283, 675, 687
BsmI GAATGC 1 cut(s) 877
BsnI GGCC 5 cut(s) 8, 68, 360, 907, 929
Bsp1286I GDGCHC 2 cut(s) 240, 646
Bsp143I GATC 1 cut(s) 388
Bsp19I CCATGG 1 cut(s) 668
BspACI CCGC 3 cut(s) 179, 222, 386
BspANI GGCC 5 cut(s) 8, 68, 360, 907, 929
BspCNI CTCAG 3 cut(s) 310, 340, 931
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 3 cut(s) 40, 127, 675
BspMAI CTGCAG 1 cut(s) 413
BspMI ACCTGC 1 cut(s) 545
BspPI GGATC 1 cut(s) 396
BspT107I GGYRCC 1 cut(s) 125
BsrDI GCAATG 1 cut(s) 312
BsrFI RCCGGY 2 cut(s) 132, 139
BsrI ACTGG 5 cut(s) 271, 634, 663, 849, 865
BssAI RCCGGY 2 cut(s) 132, 139
BssECI CCNNGG 4 cut(s) 418, 588, 668, 908
BssMI GATC 1 cut(s) 388
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 3 cut(s) 418, 668, 908
Bst2UI CCWGG 2 cut(s) 245, 590
Bst4CI ACNGT 5 cut(s) 25, 58, 106, 202, 438
Bst6I CTCTTC 1 cut(s) 679
BstACI GRCGYC 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 96
BstC8I GCNNGC 4 cut(s) 718, 737, 775, 915
BstDEI CTNAG 5 cut(s) 318, 348, 614, 918, 932
BstDSI CCRYGG 1 cut(s) 668
BstF5I GGATG 4 cut(s) 284, 428, 584, 718
BstFNI CGCG 1 cut(s) 179
BstHHI GCGC 2 cut(s) 189, 571
BstKTI GATC 1 cut(s) 391
BstMAI GTCTC 2 cut(s) 191, 587
BstMBI GATC 1 cut(s) 388
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 2 cut(s) 245, 590
BstNSI RCATGY 2 cut(s) 720, 731
BstSCI CCNGG 5 cut(s) 121, 189, 243, 588, 802
BstSFI CTRYAG 1 cut(s) 409
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 1 cut(s) 148
Bsu36I CCTNAGG 1 cut(s) 348
BsuI GTATCC 1 cut(s) 424
BsuRI GGCC 5 cut(s) 8, 68, 360, 907, 929
BtgI CCRYGG 1 cut(s) 668
BtrI CACGTC 1 cut(s) 210
BtsCI GGATG 4 cut(s) 284, 428, 584, 718
BtsI GCAGTG 1 cut(s) 541
BtsIMutI CAGTG 3 cut(s) 102, 434, 541
BveI ACCTGC 1 cut(s) 545
Cac8I GCNNGC 4 cut(s) 718, 737, 775, 915
CfoI GCGC 2 cut(s) 189, 571
Cfr10I RCCGGY 2 cut(s) 132, 139
Cfr13I GGNCC 3 cut(s) 674, 800, 928
CseI GACGC 1 cut(s) 83
Csp6I GTAC 4 cut(s) 59, 145, 288, 439
CviAII CATG 4 cut(s) 669, 717, 728, 831
CviQI GTAC 4 cut(s) 59, 145, 288, 439
DdeI CTNAG 5 cut(s) 318, 348, 614, 918, 932
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
Eam1104I CTCTTC 1 cut(s) 679
EarI CTCTTC 1 cut(s) 679
Eco130I CCWWGG 3 cut(s) 418, 668, 908
Eco147I AGGCCT 1 cut(s) 907
Eco47I GGWCC 2 cut(s) 674, 800
Eco72I CACGTG 1 cut(s) 96
Eco81I CCTNAGG 1 cut(s) 348
EcoO109I RGGNCCY 1 cut(s) 674
EcoRI GAATTC 1 cut(s) 337
EcoRII CCWGG 2 cut(s) 243, 588
EcoT14I CCWWGG 3 cut(s) 418, 668, 908
EcoT22I ATGCAT 2 cut(s) 718, 722
ErhI CCWWGG 3 cut(s) 418, 668, 908
FaeI CATG 4 cut(s) 672, 720, 731, 834
FalI AAGNNNNNCTT 2 cut(s) 757, 789
FaqI GGGAC 6 cut(s) 105, 134, 277, 283, 675, 687
FatI CATG 4 cut(s) 668, 716, 727, 830
FauI CCCGC 1 cut(s) 172
FblI GTMKAC 1 cut(s) 492
Fnu4HI GCNGC 1 cut(s) 137
FokI GGATG 4 cut(s) 291, 435, 571, 725
Fsp4HI GCNGC 1 cut(s) 137
FspBI CTAG 2 cut(s) 252, 837
FspI TGCGCA 1 cut(s) 570
GlaI GCGC 2 cut(s) 188, 570
GluI GCNGC 1 cut(s) 137
GsuI CTGGAG 1 cut(s) 857
HaeIII GGCC 5 cut(s) 8, 68, 360, 907, 929
HapII CCGG 6 cut(s) 42, 123, 133, 140, 191, 803
HgaI GACGC 1 cut(s) 83
HhaI GCGC 2 cut(s) 189, 571
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 4 cut(s) 672, 720, 731, 834
Hin6I GCGC 2 cut(s) 187, 569
HinP1I GCGC 2 cut(s) 187, 569
HinfI GANTC 2 cut(s) 109, 414
HpaII CCGG 6 cut(s) 42, 123, 133, 140, 191, 803
HphI GGTGA 3 cut(s) 46, 56, 268
Hpy166II GTNNAC 2 cut(s) 145, 493
Hpy188I TCNGA 1 cut(s) 921
Hpy188III TCNNGA 1 cut(s) 347
Hpy8I GTNNAC 2 cut(s) 145, 493
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 2 cut(s) 293, 476
HpyCH4III ACNGT 5 cut(s) 25, 58, 106, 202, 438
HpyCH4IV ACGT 3 cut(s) 95, 147, 209
HpyCH4V TGCA 8 cut(s) 90, 411, 445, 546, 698, 716, 720, 950
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 5 cut(s) 318, 348, 614, 918, 932
HpySE526I ACGT 3 cut(s) 95, 147, 209
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 4 cut(s) 672, 720, 731, 834
HspAI GCGC 2 cut(s) 187, 569
Kzo9I GATC 1 cut(s) 388
LmnI GCTCC 3 cut(s) 44, 526, 649
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 2 cut(s) 703, 729
MaeI CTAG 2 cut(s) 252, 837
MaeII ACGT 3 cut(s) 95, 147, 209
MaeIII GTNAC 4 cut(s) 27, 44, 379, 826
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MboII GAAGA 2 cut(s) 333, 696
MfeI CAATTG 1 cut(s) 291
MhlI GDGCHC 2 cut(s) 240, 646
MluCI AATT 4 cut(s) 291, 337, 372, 742
MnlI CCTC 9 cut(s) 58, 93, 176, 187, 298, 343, 632, 847, 881
Mph1103I ATGCAT 2 cut(s) 718, 722
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 6 cut(s) 42, 123, 133, 140, 191, 803
MspR9I CCNGG 5 cut(s) 123, 191, 245, 590, 804
MunI CAATTG 1 cut(s) 291
Mva1269I GAATGC 1 cut(s) 877
MvaI CCWGG 2 cut(s) 245, 590
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 1 cut(s) 74
NciI CCSGG 3 cut(s) 123, 191, 804
NcoI CCATGG 1 cut(s) 668
NdeII GATC 1 cut(s) 388
NlaIII CATG 4 cut(s) 672, 720, 731, 834
NlaIV GGNNCC 3 cut(s) 40, 127, 675
NmuCI GTSAC 3 cut(s) 27, 44, 826
NsbI TGCGCA 1 cut(s) 570
NsiI ATGCAT 2 cut(s) 718, 722
NspI RCATGY 2 cut(s) 720, 731
PaeI GCATGC 1 cut(s) 720
PceI AGGCCT 1 cut(s) 907
PciI ACATGT 1 cut(s) 727
PctI GAATGC 1 cut(s) 877
PfeI GAWTC 2 cut(s) 109, 414
PflMI CCANNNNNTGG 1 cut(s) 367
PkrI GCNGC 1 cut(s) 138
PmaCI CACGTG 1 cut(s) 96
PmlI CACGTG 1 cut(s) 96
Ppu21I YACGTR 1 cut(s) 96
PpuMI RGGWCCY 1 cut(s) 674
PscI ACATGT 1 cut(s) 727
PsiI TTATAA 1 cut(s) 405
Psp5II RGGWCCY 1 cut(s) 674
Psp6I CCWGG 2 cut(s) 243, 588
PspCI CACGTG 1 cut(s) 96
PspGI CCWGG 2 cut(s) 243, 588
PspN4I GGNNCC 3 cut(s) 40, 127, 675
PspPI GGNCC 3 cut(s) 674, 800, 928
PspPPI RGGWCCY 1 cut(s) 674
PstI CTGCAG 1 cut(s) 413
RsaI GTAC 4 cut(s) 60, 146, 289, 440
RsaNI GTAC 4 cut(s) 59, 145, 288, 439
SatI GCNGC 1 cut(s) 137
Sau3AI GATC 1 cut(s) 388
Sau96I GGNCC 3 cut(s) 674, 800, 928
ScrFI CCNGG 5 cut(s) 123, 191, 245, 590, 804
SduI GDGCHC 2 cut(s) 240, 646
SfaNI GCATC 2 cut(s) 703, 729
SfcI CTRYAG 1 cut(s) 409
SinI GGWCC 2 cut(s) 674, 800
SphI GCATGC 1 cut(s) 720
Sse9I AATT 4 cut(s) 291, 337, 372, 742
SseBI AGGCCT 1 cut(s) 907
SsiI CCGC 3 cut(s) 179, 222, 386
SspMI CTAG 2 cut(s) 252, 837
StuI AGGCCT 1 cut(s) 907
StyD4I CCNGG 5 cut(s) 121, 189, 243, 588, 802
StyI CCWWGG 3 cut(s) 418, 668, 908
TaaI ACNGT 5 cut(s) 25, 58, 106, 202, 438
TaiI ACGT 3 cut(s) 98, 150, 212
TaqI TCGA 4 cut(s) 72, 234, 823, 888
TasI AATT 4 cut(s) 291, 337, 372, 742
TatI WGTACW 2 cut(s) 287, 438
TfiI GAWTC 2 cut(s) 109, 414
TscAI CASTG 3 cut(s) 109, 441, 548
TseFI GTSAC 3 cut(s) 27, 44, 826
TseI GCWGC 1 cut(s) 136
Tsp45I GTSAC 3 cut(s) 27, 44, 826
TspDTI ATGAA 4 cut(s) 301, 550, 777, 798
TspRI CASTG 3 cut(s) 109, 441, 548
Van91I CCANNNNNTGG 1 cut(s) 367
VpaK11BI GGWCC 2 cut(s) 674, 800
XapI RAATTY 1 cut(s) 337
XceI RCATGY 2 cut(s) 720, 731
XmiI GTMKAC 1 cut(s) 492
XspI CTAG 2 cut(s) 252, 837
Zsp2I ATGCAT 2 cut(s) 718, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.