AT3G30805

lysyl-tRNA aminoacylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005056
Physical Location & Seq
Reverse (-)
12488978 .. 12489925
948 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G30805.1

Sequence Viewer

Length: 501 bp
ATGAAGTTGGAAAGTTTTGGCTTGACGTTGGAATGTCTTCAAAAGTGCAGAACGGGTTGCAAGATTTTGGGTTTGTTCATCTCAAAGTGGTTCTCATCGTCAAGGAACAAGACTGCCAGGGAAGGACCAGCTGAAGAGAACAGTTGGAAGCAGTTTGGGGTCACTCCTAAGGGTGGTAATGAGAGCTGCTCTAACCAATATGGTTGTACAGATCGACAATCTGGAGATGATGAAGCTATGGCTATGGATGAGACGTTTTGCATGGCGCTAGAATATGGATTGCCTCCTACTGGTGGTTGGGGAATGGGAATAGACAGGCCTCTTATGTTACTTACCGACTCACAGAACATTAAGGTCCCACTTTTTTTTTTCCCTGAAGCCAGTCTAGAGAAATCCAATGAAGTAGTATGTATGTTGTCTTACTCATGTTCTGAATTTTGTTGCATGAGGTTTTCTTTTTCCCAACAACGAAGCCTCATGACTAGCCAGCTGCTGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000049 GO:0000165 GO:0000166 GO:0000187 GO:0001775 GO:0001817 GO:0001819 GO:0001932 GO:0001934 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002276 GO:0002366 GO:0002376 GO:0002682 GO:0002684 GO:0002685 GO:0002687 GO:0002688 GO:0002690 GO:0002694 GO:0002696 GO:0002697 GO:0002699 GO:0002700 GO:0002702 GO:0002718 GO:0002720 GO:0002739 GO:0002741 GO:0002791 GO:0002793 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0003877 GO:0004812 GO:0004824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0005911 GO:0006082 GO:0006139 GO:0006355 GO:0006396 GO:0006399 GO:0006412 GO:0006418 GO:0006430 GO:0006464 GO:0006468 GO:0006518 GO:0006520 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007275 GO:0007568 GO:0008033 GO:0008144 GO:0008150 GO:0008152 GO:0008285 GO:0008340 GO:0009058 GO:0009059 GO:0009117 GO:0009165 GO:0009314 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009791 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009908 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010165 GO:0010212 GO:0010259 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010646 GO:0010647 GO:0010758 GO:0010759 GO:0010952 GO:0015959 GO:0015960 GO:0015965 GO:0015966 GO:0016070 GO:0016310 GO:0016597 GO:0016740 GO:0016772 GO:0016779 GO:0016874 GO:0016875 GO:0017076 GO:0017101 GO:0018130 GO:0019219 GO:0019220 GO:0019221 GO:0019222 GO:0019438 GO:0019538 GO:0019637 GO:0019752 GO:0022414 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030054 GO:0030162 GO:0030334 GO:0030335 GO:0030554 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0031399 GO:0031401 GO:0031406 GO:0031974 GO:0032101 GO:0032103 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032504 GO:0032553 GO:0032555 GO:0032559 GO:0032872 GO:0032874 GO:0032879 GO:0032880 GO:0032991 GO:0033209 GO:0033674 GO:0034097 GO:0034470 GO:0034612 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0035639 GO:0035670 GO:0036094 GO:0036211 GO:0036230 GO:0040012 GO:0040017 GO:0042127 GO:0042221 GO:0042325 GO:0042327 GO:0042802 GO:0042803 GO:0043030 GO:0043032 GO:0043038 GO:0043039 GO:0043043 GO:0043085 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043436 GO:0043549 GO:0043603 GO:0043604 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044421 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0045321 GO:0045575 GO:0045859 GO:0045860 GO:0045862 GO:0045893 GO:0045935 GO:0045937 GO:0046483 GO:0046983 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048582 GO:0048583 GO:0048584 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048856 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050727 GO:0050729 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050865 GO:0050867 GO:0050896 GO:0050920 GO:0050921 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051094 GO:0051171 GO:0051173 GO:0051174 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051270 GO:0051272 GO:0051336 GO:0051338 GO:0051345 GO:0051347 GO:0051704 GO:0051716 GO:0052547 GO:0055044 GO:0055086 GO:0060255 GO:0060378 GO:0061062 GO:0061063 GO:0061458 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070201 GO:0070302 GO:0070304 GO:0070371 GO:0070372 GO:0070374 GO:0070566 GO:0070887 GO:0071310 GO:0071345 GO:0071356 GO:0071622 GO:0071675 GO:0071704 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0090087 GO:0090304 GO:0090407 GO:0090567 GO:0097110 GO:0097159 GO:0097367 GO:0099402 GO:0140098 GO:0140101 GO:1900015 GO:1900017 GO:1900744 GO:1900745 GO:1901265 GO:1901293 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1902531 GO:1902533 GO:1902680 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1905048 GO:1905050 GO:1905521 GO:1905523 GO:2000026 GO:2000112 GO:2000145 GO:2000147 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
tRNA-synt_2 tRNA_anti-codon

Protein Analysis

166

Amino Acids

18.59

Weight (kDa)

5.08

Isoelectric Point (pI)

60.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_2 PF00152 73 - 123 5.9e-13 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 434
AcuI CTGAAG 2 cut(s) 153, 396
AfaI GTAC 1 cut(s) 208
AfiI CCNNNNNNNGG 3 cut(s) 173, 290, 293
AgsI TTSAA 1 cut(s) 41
AjnI CCWGG 1 cut(s) 116
AluBI AGCT 4 cut(s) 131, 186, 236, 490
AluI AGCT 4 cut(s) 131, 186, 236, 490
Alw26I GTCTC 1 cut(s) 245
AlwNI CAGNNNCTG 1 cut(s) 493
AoxI GGCC 1 cut(s) 317
ApeKI GCWGC 3 cut(s) 186, 490, 493
ApoI RAATTY 1 cut(s) 434
Asp700I GAANNNNTTC 1 cut(s) 36
AspLEI GCGC 1 cut(s) 268
AspS9I GGNCC 2 cut(s) 125, 355
AvaII GGWCC 2 cut(s) 125, 355
AxyI CCTNAGG 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 29
BbvI GCAGC 3 cut(s) 173, 477, 480
BciT130I CCWGG 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 245
BfaI CTAG 3 cut(s) 269, 386, 483
BfoI RGCGCY 1 cut(s) 269
BisI GCNGC 3 cut(s) 187, 491, 494
BlsI GCNGC 3 cut(s) 188, 492, 495
Bme1390I CCNGG 1 cut(s) 118
Bme18I GGWCC 2 cut(s) 125, 355
BmgT120I GGNCC 2 cut(s) 125, 355
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 1 cut(s) 118
BpiI GAAGAC 1 cut(s) 29
BplI GAGNNNNNCTC 2 cut(s) 173, 205
BpmI CTGGAG 1 cut(s) 243
BsaJI CCNNGG 1 cut(s) 117
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 290, 293
Bse1I ACTGG 2 cut(s) 295, 381
Bse21I CCTNAGG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 118
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 1 cut(s) 253
BseLI CCNNNNNNNGG 3 cut(s) 173, 290, 293
BseNI ACTGG 2 cut(s) 295, 381
BseXI GCAGC 3 cut(s) 173, 477, 480
BsgI GTGCAG 1 cut(s) 67
BshFI GGCC 1 cut(s) 319
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 3 cut(s) 173, 290, 293
BsmAI GTCTC 1 cut(s) 245
BsmBI CGTCTC 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 341
BsnI GGCC 1 cut(s) 319
Bsp1407I TGTACA 1 cut(s) 206
Bsp143I GATC 1 cut(s) 211
BspANI GGCC 1 cut(s) 319
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 1 cut(s) 357
BsrGI TGTACA 1 cut(s) 206
BsrI ACTGG 2 cut(s) 295, 381
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 1 cut(s) 211
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 129
BstAUI TGTACA 1 cut(s) 206
BstC8I GCNNGC 1 cut(s) 488
BstDEI CTNAG 1 cut(s) 168
BstF5I GGATG 1 cut(s) 253
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 1 cut(s) 214
BstMAI GTCTC 1 cut(s) 245
BstMBI GATC 1 cut(s) 211
BstNI CCWGG 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 116
BstV1I GCAGC 3 cut(s) 173, 477, 480
BstV2I GAAGAC 1 cut(s) 29
Bsu36I CCTNAGG 1 cut(s) 168
BsuRI GGCC 1 cut(s) 319
BtsCI GGATG 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 488
CaiI CAGNNNCTG 1 cut(s) 493
CciI TCATGA 1 cut(s) 477
CfoI GCGC 1 cut(s) 268
Cfr13I GGNCC 2 cut(s) 125, 355
Csp6I GTAC 1 cut(s) 207
CviAII CATG 4 cut(s) 262, 426, 445, 478
CviQI GTAC 1 cut(s) 207
DdeI CTNAG 1 cut(s) 168
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Eco147I AGGCCT 1 cut(s) 319
Eco47I GGWCC 2 cut(s) 125, 355
Eco57I CTGAAG 2 cut(s) 153, 396
Eco81I CCTNAGG 1 cut(s) 168
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 1 cut(s) 116
Esp3I CGTCTC 1 cut(s) 245
FaeI CATG 4 cut(s) 265, 429, 448, 481
FaqI GGGAC 1 cut(s) 341
FatI CATG 4 cut(s) 261, 425, 444, 477
Fnu4HI GCNGC 3 cut(s) 187, 491, 494
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 3 cut(s) 187, 491, 494
FspBI CTAG 3 cut(s) 269, 386, 483
GlaI GCGC 1 cut(s) 267
GluI GCNGC 3 cut(s) 187, 491, 494
GsuI CTGGAG 1 cut(s) 243
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 1 cut(s) 319
HhaI GCGC 1 cut(s) 268
Hin1II CATG 4 cut(s) 265, 429, 448, 481
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HinfI GANTC 1 cut(s) 338
Hpy188I TCNGA 1 cut(s) 433
Hpy188III TCNNGA 3 cut(s) 222, 386, 478
HpyAV CCTTC 1 cut(s) 116
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4IV ACGT 2 cut(s) 26, 254
HpyCH4V TGCA 5 cut(s) 48, 60, 261, 444, 496
HpyF3I CTNAG 1 cut(s) 168
HpySE526I ACGT 2 cut(s) 26, 254
Hsp92II CATG 4 cut(s) 265, 429, 448, 481
HspAI GCGC 1 cut(s) 266
Kzo9I GATC 1 cut(s) 211
LpnPI CCDG 8 cut(s) 103, 130, 141, 207, 276, 301, 387, 394
Lsp1109I GCAGC 3 cut(s) 173, 477, 480
MaeI CTAG 3 cut(s) 269, 386, 483
MaeII ACGT 2 cut(s) 26, 254
MaeIII GTNAC 2 cut(s) 160, 327
MalI GATC 1 cut(s) 213
MboI GATC 1 cut(s) 211
MboII GAAGA 2 cut(s) 29, 146
MluCI AATT 1 cut(s) 434
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 2 cut(s) 9, 125
MnlI CCTC 4 cut(s) 294, 330, 441, 485
MroXI GAANNNNTTC 1 cut(s) 36
MseI TTAA 1 cut(s) 351
MspA1I CMGCKG 2 cut(s) 131, 490
MspR9I CCNGG 1 cut(s) 118
MvaI CCWGG 1 cut(s) 118
NdeII GATC 1 cut(s) 211
NlaIII CATG 4 cut(s) 265, 429, 448, 481
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 1 cut(s) 160
PagI TCATGA 1 cut(s) 477
PceI AGGCCT 1 cut(s) 319
PdmI GAANNNNTTC 1 cut(s) 36
PkrI GCNGC 3 cut(s) 188, 492, 495
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
PpuMI RGGWCCY 1 cut(s) 355
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 116
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 1 cut(s) 357
PspPI GGNCC 2 cut(s) 125, 355
PspPPI RGGWCCY 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 493
PvuII CAGCTG 2 cut(s) 131, 490
RsaI GTAC 1 cut(s) 208
RsaNI GTAC 1 cut(s) 207
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 3 cut(s) 187, 491, 494
Sau3AI GATC 1 cut(s) 211
Sau96I GGNCC 2 cut(s) 125, 355
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 118
SetI ASST 8 cut(s) 29, 133, 188, 238, 257, 357, 452, 492
SinI GGWCC 2 cut(s) 125, 355
Sse9I AATT 1 cut(s) 434
SseBI AGGCCT 1 cut(s) 319
SspMI CTAG 3 cut(s) 269, 386, 483
StuI AGGCCT 1 cut(s) 319
StyD4I CCNGG 1 cut(s) 116
TaaI ACNGT 1 cut(s) 143
TaiI ACGT 2 cut(s) 29, 257
TaqI TCGA 1 cut(s) 214
TasI AATT 1 cut(s) 434
TatI WGTACW 1 cut(s) 206
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 3 cut(s) 186, 490, 493
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 4 cut(s) 17, 67, 246, 414
VpaK11BI GGWCC 2 cut(s) 125, 355
XapI RAATTY 1 cut(s) 434
XbaI TCTAGA 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 36
XspI CTAG 3 cut(s) 269, 386, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.