MD13G1247500.v1.1

lysine--tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005056
Physical Location & Seq
Reverse (-)
26172548 .. 26173487
940 bp
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UTR
Exon/CDS
Intron
MD13G1247500.v1.1.491

Sequence Viewer

Length: 351 bp
ATGAATCAGGATACTACCTCTGGTTTTGTGTGCAGGAGGAGTGCCATTGCAATCAATGCAATGTTATGTTTTTCTCCTTGCCAATGCATACACAGAGTTGAAGATCCTGTGGTACAACGCCAACGATTTGCTGATCAACTCAAGGTCCAACAACCAAGTGATGATGAAGCAATAGCTTTGGACGAATCATTCTGTAGAGCTCTTGAGTACGGGTTACCTCCAAATGGTGGTTCGGGTTTGGGTGTTGATCGATGTGTGTGTGGTCAACTGATTCACAAAATATCAAGGTTTGTGTGTGTGTGTGTGTATCTCTCTCTCTCTCCAATCTTGAGTACGTTATGTGTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00970 map00970
Pfam Domains
Protein Families
tRNA-synt_2 tRNA_anti-codon

Protein Analysis

117

Amino Acids

12.74

Weight (kDa)

6.04

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_2 PF00152 31 - 87 6.8e-10 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 227
AclWI GGATC 1 cut(s) 98
AfaI GTAC 3 cut(s) 114, 209, 334
AfiI CCNNNNNNNGG 2 cut(s) 224, 227
AgsI TTSAA 1 cut(s) 101
AjuI GAANNNNNNNTTGG 2 cut(s) 214, 246
AluBI AGCT 2 cut(s) 176, 200
AluI AGCT 2 cut(s) 176, 200
Alw21I GWGCWC 1 cut(s) 202
AlwI GGATC 1 cut(s) 98
AspS9I GGNCC 1 cut(s) 145
AvaII GGWCC 1 cut(s) 145
BanII GRGCYC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 202
BciVI GTATCC 1 cut(s) 4
BclI TGATCA 1 cut(s) 133
BfaI CTAG 1 cut(s) 349
BfmI CTRYAG 1 cut(s) 193
BfuI GTATCC 1 cut(s) 4
Bme18I GGWCC 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 145
BpuEI CTTGAG 3 cut(s) 125, 224, 349
Bsa29I ATCGAT 1 cut(s) 250
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 227
Bse3DI GCAATG 2 cut(s) 45, 66
BseCI ATCGAT 1 cut(s) 250
BseLI CCNNNNNNNGG 2 cut(s) 224, 227
BseMI GCAATG 2 cut(s) 45, 66
BseRI GAGGAG 1 cut(s) 52
BsgI GTGCAG 1 cut(s) 52
BshVI ATCGAT 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 202
BslI CCNNNNNNNGG 2 cut(s) 224, 227
Bsp1286I GDGCHC 1 cut(s) 202
Bsp143I GATC 3 cut(s) 103, 133, 247
BspDI ATCGAT 1 cut(s) 250
BspPI GGATC 1 cut(s) 98
BsrDI GCAATG 2 cut(s) 45, 66
BssMI GATC 3 cut(s) 103, 133, 247
BstAPI GCANNNNNTGC 1 cut(s) 56
BstEII GGTNACC 1 cut(s) 213
BstKTI GATC 3 cut(s) 106, 136, 250
BstMBI GATC 3 cut(s) 103, 133, 247
BstMWI GCNNNNNNNGC 1 cut(s) 56
BstPI GGTNACC 1 cut(s) 213
BstSFI CTRYAG 1 cut(s) 193
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
Bsu15I ATCGAT 1 cut(s) 250
BsuI GTATCC 1 cut(s) 4
BsuTUI ATCGAT 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 145
ClaI ATCGAT 1 cut(s) 250
Csp6I GTAC 3 cut(s) 113, 208, 333
CviJI RGCY 2 cut(s) 176, 200
CviKI_1 RGCY 2 cut(s) 176, 200
CviQI GTAC 3 cut(s) 113, 208, 333
DpnI GATC 3 cut(s) 105, 135, 249
DpnII GATC 3 cut(s) 103, 133, 247
Ecl136II GAGCTC 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 202
Eco47I GGWCC 1 cut(s) 145
Eco53kI GAGCTC 1 cut(s) 200
Eco91I GGTNACC 1 cut(s) 213
EcoICRI GAGCTC 1 cut(s) 200
EcoO65I GGTNACC 1 cut(s) 213
EcoT22I ATGCAT 1 cut(s) 89
EcoT38I GRGCYC 1 cut(s) 202
FaiI YATR 3 cut(s) 67, 89, 340
FbaI TGATCA 1 cut(s) 133
FriOI GRGCYC 1 cut(s) 202
FspBI CTAG 1 cut(s) 349
HincII GTYRAC 1 cut(s) 266
HindII GTYRAC 1 cut(s) 266
HinfI GANTC 3 cut(s) 4, 185, 271
Hpy166II GTNNAC 2 cut(s) 266, 346
Hpy188III TCNNGA 3 cut(s) 8, 203, 328
Hpy8I GTNNAC 2 cut(s) 266, 346
HpyCH4IV ACGT 1 cut(s) 335
HpyCH4V TGCA 4 cut(s) 33, 50, 59, 87
HpyF10VI GCNNNNNNNGC 1 cut(s) 56
HpySE526I ACGT 1 cut(s) 335
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 3 cut(s) 103, 133, 247
LpnPI CCDG 3 cut(s) 6, 19, 120
MaeI CTAG 1 cut(s) 349
MaeII ACGT 1 cut(s) 335
MaeIII GTNAC 1 cut(s) 213
MalI GATC 3 cut(s) 105, 135, 249
MboI GATC 3 cut(s) 103, 133, 247
MboII GAAGA 1 cut(s) 113
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 1 cut(s) 202
MmeI TCCRAC 1 cut(s) 172
MnlI CCTC 3 cut(s) 28, 30, 228
Mph1103I ATGCAT 1 cut(s) 89
MwoI GCNNNNNNNGC 1 cut(s) 56
NdeII GATC 3 cut(s) 103, 133, 247
NsiI ATGCAT 1 cut(s) 89
PfeI GAWTC 3 cut(s) 4, 185, 271
PflMI CCANNNNNTGG 1 cut(s) 227
Psp124BI GAGCTC 1 cut(s) 202
PspEI GGTNACC 1 cut(s) 213
PspPI GGNCC 1 cut(s) 145
PsuI RGATCY 1 cut(s) 103
RsaI GTAC 3 cut(s) 114, 209, 334
RsaNI GTAC 3 cut(s) 113, 208, 333
SacI GAGCTC 1 cut(s) 202
Sau3AI GATC 3 cut(s) 103, 133, 247
Sau96I GGNCC 1 cut(s) 145
SduI GDGCHC 1 cut(s) 202
SetI ASST 7 cut(s) 20, 147, 178, 202, 220, 290, 338
SfcI CTRYAG 1 cut(s) 193
SinI GGWCC 1 cut(s) 145
SmlI CTYRAG 3 cut(s) 140, 203, 328
SmoI CTYRAG 3 cut(s) 140, 203, 328
SspMI CTAG 1 cut(s) 349
SstI GAGCTC 1 cut(s) 202
TaiI ACGT 1 cut(s) 338
TaqI TCGA 1 cut(s) 250
TfiI GAWTC 3 cut(s) 4, 185, 271
TspDTI ATGAA 2 cut(s) 17, 180
Van91I CCANNNNNTGG 1 cut(s) 227
VpaK11BI GGWCC 1 cut(s) 145
XspI CTAG 1 cut(s) 349
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.