Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005056

Physical Location & Seq

Genomic Coordinates Reverse (-)

23189414 .. 23190520

Gene Structure 1107 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1170800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 633 bp

ATGTTAAGAAACTTATTTGGTGGTTTTAACTGTAACTACTGCAGAAGGATCGACATGGTTGAGGAATTAAATAAGATTGGAGGTCTCAACATAAATCCAGAGGATCTCTCTAGTGCGGAAGCTAACCAATATCTTAAGGATGTGTGCAAGAAGTTTGATATCAAATGTTCCCCTCCTGAAACAACAACTCGTTTGTTAGACAAGCTTGTGGGGCACTTGTTGGAAGAGACGTGTGTAAATCCTGCGTTCATCATTAACCATCCTGAGATAATGAGTCCTTTAGCAAAGTGGCACCGATCAAAACGTGGCCTGACTGAACGTTTTGAATTGTTTATCAACAAGCATGAACTTGCCAATGCATACACAAAATTGAATGATCCTGTGGTACAACGCCAACGATTTGCTGATCAACTCAAGGTGATGATGAAGCAATGGTTTTGGACGAAGCATAGAGCTGTTGAGTACGGGTTACCTCCAACTGGTGGTTGGGGTTTGGTCATTGATCGACTCTGTATGTGGCTAACTGATTCACAAAATAATAAGGAAGTTCTACTCTTCCCGGCTATGAAACCACAGGATGAGCCTTCTGCTAAAGGTACTAACTTCTTTTGGAATAACTTTCCTCGAACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0009506 GO:0016874 GO:0016875 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0140098 GO:0140101

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2 tRNA_anti-codon

Protein Analysis

211

Amino Acids

24.43

Weight (kDa)

8.71

Isoelectric Point (pI)

47.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2	PF00152	57 - 189	1.1e-28	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005056)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11710 AT3G30805
fragaria_vesca	FvH4_2g08732 FvH4_4g07860 FvH4_4g07860 FvH4_4g07860 FvH4_6g23270
malus_domestica	MD03G1170800.v1.1 MD13G1247500.v1.1 MD13G1247600.v1.1 MD13G1248200.v1.1 MD16G1255000.v1.1
prunus_persica	Prupe.1G082400_v2.0.a1 Prupe.1G082400_v2.0.a1 Prupe.1G083700_v2.0.a1
pyrus_communis	pycom03g12770 pycom13g21870 pycom13g21930 pycom16g21650
rosa_chinensis	RchiOBHm_Chr4g0401131
rosa_laevigata	RLG00000009161
rosa_multiflora	Rmu_sc0001909.1_g000026 Rmu_sc0037183.1_g000001
rosa_roxburghii	Rroxscaffold_5G00345980
rosa_rugosa	Rorug02G0525300 Rorug04G0028100
rosa_samantha	Rh2DG614600 Rh4BG100600 Rh4DG097900
rosa_wichuraiana	Rw4G008540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	291
AccB7I	CCANNNNNTGG	1 cut(s)	482
AciI	CCGC	1 cut(s)	116
AclI	AACGTT	1 cut(s)	319
AclWI	GGATC	3 cut(s)	56, 111, 371
AfaI	GTAC	3 cut(s)	387, 464, 598
AfiI	CCNNNNNNNGG	2 cut(s)	479, 482
AflII	CTTAAG	1 cut(s)	134
AflIII	ACRYGT	1 cut(s)	230
AgsI	TTSAA	2 cut(s)	326, 373
AjiI	CACGTC	1 cut(s)	231
AluBI	AGCT	3 cut(s)	122, 205, 455
AluI	AGCT	3 cut(s)	122, 205, 455
Alw26I	GTCTC	2 cut(s)	89, 221
AlwI	GGATC	3 cut(s)	56, 111, 371
AoxI	GGCC	1 cut(s)	307
AsuC2I	CCSGG	1 cut(s)	560
AsuHPI	GGTGA	1 cut(s)	430
BaeGI	GKGCMC	1 cut(s)	216
BanI	GGYRCC	1 cut(s)	291
BccI	CCATC	1 cut(s)	267
BcgI	CGANNNNNNTGC	2 cut(s)	31, 65
BclI	TGATCA	1 cut(s)	406
BcnI	CCSGG	1 cut(s)	560
BcoDI	GTCTC	2 cut(s)	89, 221
BfaI	CTAG	1 cut(s)	111
BfmI	CTRYAG	1 cut(s)	40
BfrI	CTTAAG	1 cut(s)	134
Bme1390I	CCNGG	1 cut(s)	560
BmgBI	CACGTC	1 cut(s)	231
BmiI	GGNNCC	1 cut(s)	293
BmrFI	CCNGG	1 cut(s)	560
BplI	GAGNNNNNCTC	2 cut(s)	92, 124
BpuEI	CTTGAG	1 cut(s)	398
BpuMI	CCSGG	1 cut(s)	560
BsaI	GGTCTC	1 cut(s)	89
Bsc4I	CCNNNNNNNGG	2 cut(s)	479, 482
Bse1I	ACTGG	1 cut(s)	484
Bse3DI	GCAATG	1 cut(s)	437
BseGI	GGATG	3 cut(s)	145, 259, 583
BseLI	CCNNNNNNNGG	2 cut(s)	479, 482
BseMI	GCAATG	1 cut(s)	437
BseMII	CTCAG	1 cut(s)	255
BseNI	ACTGG	1 cut(s)	484
BseSI	GKGCMC	1 cut(s)	216
BshFI	GGCC	1 cut(s)	309
BshNI	GGYRCC	1 cut(s)	291
BsiSI	CCGG	1 cut(s)	560
BslI	CCNNNNNNNGG	2 cut(s)	479, 482
BsmAI	GTCTC	2 cut(s)	89, 221
BsmBI	CGTCTC	1 cut(s)	221
BsnI	GGCC	1 cut(s)	309
Bso31I	GGTCTC	1 cut(s)	89
Bsp1286I	GDGCHC	1 cut(s)	216
Bsp143I	GATC	6 cut(s)	48, 103, 296, 376, 406, 502
BspACI	CCGC	1 cut(s)	116
BspANI	GGCC	1 cut(s)	309
BspCNI	CTCAG	1 cut(s)	256
BspLI	GGNNCC	1 cut(s)	293
BspMAI	CTGCAG	1 cut(s)	44
BspPI	GGATC	3 cut(s)	56, 111, 371
BspT107I	GGYRCC	1 cut(s)	291
BspTI	CTTAAG	1 cut(s)	134
BspTNI	GGTCTC	1 cut(s)	89
BsrDI	GCAATG	1 cut(s)	437
BsrI	ACTGG	1 cut(s)	484
BssMI	GATC	6 cut(s)	48, 103, 296, 376, 406, 502
Bst4CI	ACNGT	1 cut(s)	32
Bst6I	CTCTTC	2 cut(s)	219, 560
BstAFI	CTTAAG	1 cut(s)	134
BstDEI	CTNAG	1 cut(s)	264
BstEII	GGTNACC	1 cut(s)	468
BstF5I	GGATG	3 cut(s)	145, 259, 583
BstKTI	GATC	6 cut(s)	51, 106, 299, 379, 409, 505
BstMAI	GTCTC	2 cut(s)	89, 221
BstMBI	GATC	6 cut(s)	48, 103, 296, 376, 406, 502
BstMWI	GCNNNNNNNGC	1 cut(s)	211
BstPI	GGTNACC	1 cut(s)	468
BstSCI	CCNGG	1 cut(s)	558
BstSFI	CTRYAG	1 cut(s)	40
BstSLI	GKGCMC	1 cut(s)	216
BstX2I	RGATCY	1 cut(s)	103
BstYI	RGATCY	1 cut(s)	103
BsuRI	GGCC	1 cut(s)	309
BtrI	CACGTC	1 cut(s)	231
BtsCI	GGATG	3 cut(s)	145, 259, 583
Csp6I	GTAC	3 cut(s)	386, 463, 597
CviAII	CATG	2 cut(s)	55, 344
CviJI	RGCY	7 cut(s)	122, 205, 309, 455, 520, 563, 583
CviKI_1	RGCY	7 cut(s)	122, 205, 309, 455, 520, 563, 583
CviQI	GTAC	3 cut(s)	386, 463, 597
DdeI	CTNAG	1 cut(s)	264
DpnI	GATC	6 cut(s)	50, 105, 298, 378, 408, 504
DpnII	GATC	6 cut(s)	48, 103, 296, 376, 406, 502
Eam1104I	CTCTTC	2 cut(s)	219, 560
EarI	CTCTTC	2 cut(s)	219, 560
Eco31I	GGTCTC	1 cut(s)	89
Eco32I	GATATC	1 cut(s)	160
Eco91I	GGTNACC	1 cut(s)	468
EcoO65I	GGTNACC	1 cut(s)	468
EcoRV	GATATC	1 cut(s)	160
EcoT22I	ATGCAT	1 cut(s)	361
Esp3I	CGTCTC	1 cut(s)	221
FaeI	CATG	2 cut(s)	58, 347
FaiI	YATR	7 cut(s)	56, 92, 345, 361, 450, 515, 566
FatI	CATG	2 cut(s)	54, 343
FbaI	TGATCA	1 cut(s)	406
FokI	GGATG	3 cut(s)	152, 246, 590
FspBI	CTAG	1 cut(s)	111
HaeIII	GGCC	1 cut(s)	309
HapII	CCGG	1 cut(s)	560
Hin1II	CATG	2 cut(s)	58, 347
HindIII	AAGCTT	1 cut(s)	203
HinfI	GANTC	3 cut(s)	274, 507, 527
HpaII	CCGG	1 cut(s)	560
HphI	GGTGA	1 cut(s)	430
Hpy188III	TCNNGA	3 cut(s)	98, 176, 263
HpyAV	CCTTC	2 cut(s)	39, 594
HpyCH4III	ACNGT	1 cut(s)	32
HpyCH4IV	ACGT	4 cut(s)	230, 304, 319, 629
HpyCH4V	TGCA	3 cut(s)	42, 147, 359
HpyF10VI	GCNNNNNNNGC	1 cut(s)	211
HpyF3I	CTNAG	1 cut(s)	264
HpySE526I	ACGT	4 cut(s)	230, 304, 319, 629
Hsp92II	CATG	2 cut(s)	58, 347
Ksp22I	TGATCA	1 cut(s)	406
Kzo9I	GATC	6 cut(s)	48, 103, 296, 376, 406, 502
LpnPI	CCDG	9 cut(s)	111, 189, 255, 276, 323, 393, 465, 560, 573
MaeI	CTAG	1 cut(s)	111
MaeII	ACGT	4 cut(s)	230, 304, 319, 629
MaeIII	GTNAC	2 cut(s)	32, 468
MalI	GATC	6 cut(s)	50, 105, 298, 378, 408, 504
MboI	GATC	6 cut(s)	48, 103, 296, 376, 406, 502
MboII	GAAGA	2 cut(s)	236, 547
MflI	RGATCY	1 cut(s)	103
MhlI	GDGCHC	1 cut(s)	216
MluCI	AATT	3 cut(s)	65, 326, 368
MlyI	GAGTC	2 cut(s)	283, 501
MmeI	TCCRAC	2 cut(s)	201, 500
MnlI	CCTC	6 cut(s)	55, 74, 94, 183, 483, 633
Mph1103I	ATGCAT	1 cut(s)	361
MseI	TTAA	5 cut(s)	5, 27, 68, 135, 255
MspCI	CTTAAG	1 cut(s)	134
MspI	CCGG	1 cut(s)	560
MspR9I	CCNGG	1 cut(s)	560
MwoI	GCNNNNNNNGC	1 cut(s)	211
NciI	CCSGG	1 cut(s)	560
NdeII	GATC	6 cut(s)	48, 103, 296, 376, 406, 502
NlaIII	CATG	2 cut(s)	58, 347
NlaIV	GGNNCC	1 cut(s)	293
NsiI	ATGCAT	1 cut(s)	361
PfeI	GAWTC	1 cut(s)	527
PflMI	CCANNNNNTGG	1 cut(s)	482
PleI	GAGTC	2 cut(s)	282, 501
PpsI	GAGTC	2 cut(s)	282, 501
Psp1406I	AACGTT	1 cut(s)	319
PspEI	GGTNACC	1 cut(s)	468
PspN4I	GGNNCC	1 cut(s)	293
PstI	CTGCAG	1 cut(s)	44
PsuI	RGATCY	1 cut(s)	103
RsaI	GTAC	3 cut(s)	387, 464, 598
RsaNI	GTAC	3 cut(s)	386, 463, 597
SaqAI	TTAA	5 cut(s)	5, 27, 68, 135, 255
Sau3AI	GATC	6 cut(s)	48, 103, 296, 376, 406, 502
SchI	GAGTC	2 cut(s)	283, 501
ScrFI	CCNGG	1 cut(s)	560
SduI	GDGCHC	1 cut(s)	216
SfcI	CTRYAG	1 cut(s)	40
SmlI	CTYRAG	2 cut(s)	134, 413
SmoI	CTYRAG	2 cut(s)	134, 413
Sse9I	AATT	3 cut(s)	65, 326, 368
SsiI	CCGC	1 cut(s)	116
SspMI	CTAG	1 cut(s)	111
StyD4I	CCNGG	1 cut(s)	558
TaaI	ACNGT	1 cut(s)	32
TaiI	ACGT	4 cut(s)	233, 307, 322, 632
TaqI	TCGA	3 cut(s)	51, 505, 625
TasI	AATT	3 cut(s)	65, 326, 368
TfiI	GAWTC	1 cut(s)	527
Tru1I	TTAA	5 cut(s)	5, 27, 68, 135, 255
Tru9I	TTAA	5 cut(s)	5, 27, 68, 135, 255
TspDTI	ATGAA	4 cut(s)	238, 360, 440, 581
Van91I	CCANNNNNTGG	1 cut(s)	482
Vha464I	CTTAAG	1 cut(s)	134
XcmI	CCANNNNNNNNNTGG	1 cut(s)	483
XspI	CTAG	1 cut(s)	111
Zsp2I	ATGCAT	1 cut(s)	361

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.