pycom13g21870

Lysyl-tRNA synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005056
Physical Location & Seq
Reverse (-)
18918069 .. 18918642
574 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g21870.1

Sequence Viewer

Length: 426 bp
ATGGTTATGGAGAATGACTCGTGCATCAGTTTATACCTTTTAGTTCATTTTCTATTTATGCAGCTTGTGGGGCAGTTTTTGGAACAGACATGTGTAAATCTCGCATTCATCATTAACCATCCTGAGATAATGAGTCCTTTAGCTAAGTGGCATCGATCGAAACCTGGCTTTACTGAACGTTATGACTTGTTCGTCAACAAGCATGAAGTATCTTGCCAATGCATACACAGAGTTGAAGATCCTGTGGTACAACGCCAACGATTTGCTGATCAACTCAAGGAATCACTTAATTGTTTAGTTATGGACCGACAACCAGGTGATGATGAAGCAATGGCTTTGGACGAATCATTCTGTAGAGCTCTTGAGTACGGGTTACCTCCAACTGGTGGTTCGGGTTTGGGCGTTGATCGCTCTGTATGTGGTTAA

Protein Analysis

142

Amino Acids

16.02

Weight (kDa)

5.1

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_2 PF00152 29 - 139 5e-20 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AccB7I CCANNNNNTGG 1 cut(s) 386
AclI AACGTT 1 cut(s) 178
AclWI GGATC 1 cut(s) 233
AfaI GTAC 2 cut(s) 249, 368
AfiI CCNNNNNNNGG 2 cut(s) 383, 386
AflIII ACRYGT 1 cut(s) 89
AgsI TTSAA 1 cut(s) 236
AjnI CCWGG 2 cut(s) 163, 313
AjuI GAANNNNNNNTTGG 2 cut(s) 373, 405
AluBI AGCT 3 cut(s) 64, 143, 359
AluI AGCT 3 cut(s) 64, 143, 359
Alw21I GWGCWC 1 cut(s) 361
AlwI GGATC 1 cut(s) 233
ApeKI GCWGC 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 304
AsuHPI GGTGA 1 cut(s) 329
AvaII GGWCC 1 cut(s) 304
BanII GRGCYC 1 cut(s) 361
BauI CACGAG 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 1 cut(s) 73
BccI CCATC 1 cut(s) 126
BciT130I CCWGG 2 cut(s) 165, 315
BclI TGATCA 1 cut(s) 268
BfmI CTRYAG 1 cut(s) 352
BisI GCNGC 1 cut(s) 62
BlsI GCNGC 1 cut(s) 63
Bme1390I CCNGG 2 cut(s) 165, 315
Bme18I GGWCC 1 cut(s) 304
BmgT120I GGNCC 1 cut(s) 304
BmrFI CCNGG 2 cut(s) 165, 315
BmsI GCATC 2 cut(s) 33, 160
BplI GAGNNNNNCTC 1 cut(s) 34
BpuEI CTTGAG 2 cut(s) 260, 383
Bsa29I ATCGAT 1 cut(s) 154
Bsc4I CCNNNNNNNGG 2 cut(s) 383, 386
Bse1I ACTGG 1 cut(s) 388
Bse3DI GCAATG 1 cut(s) 336
BseBI CCWGG 2 cut(s) 165, 315
BseCI ATCGAT 1 cut(s) 154
BseGI GGATG 1 cut(s) 118
BseLI CCNNNNNNNGG 2 cut(s) 383, 386
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 1 cut(s) 388
BseXI GCAGC 1 cut(s) 73
Bsh1285I CGRYCG 1 cut(s) 158
BshVI ATCGAT 1 cut(s) 154
BsiEI CGRYCG 1 cut(s) 158
BsiHKAI GWGCWC 1 cut(s) 361
BslI CCNNNNNNNGG 2 cut(s) 383, 386
BsmI GAATGC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 4 cut(s) 155, 238, 268, 406
BspCNI CTCAG 1 cut(s) 115
BspDI ATCGAT 1 cut(s) 154
BspPI GGATC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 336
BsrI ACTGG 1 cut(s) 388
BssMI GATC 4 cut(s) 155, 238, 268, 406
BssSI CACGAG 1 cut(s) 19
Bst2BI CACGAG 1 cut(s) 19
Bst2UI CCWGG 2 cut(s) 165, 315
BstDEI CTNAG 2 cut(s) 123, 144
BstEII GGTNACC 1 cut(s) 372
BstF5I GGATG 1 cut(s) 118
BstKTI GATC 4 cut(s) 158, 241, 271, 409
BstMBI GATC 4 cut(s) 155, 238, 268, 406
BstMCI CGRYCG 1 cut(s) 158
BstMWI GCNNNNNNNGC 2 cut(s) 70, 408
BstNI CCWGG 2 cut(s) 165, 315
BstNSI RCATGY 1 cut(s) 93
BstPI GGTNACC 1 cut(s) 372
BstSCI CCNGG 2 cut(s) 163, 313
BstSFI CTRYAG 1 cut(s) 352
BstV1I GCAGC 1 cut(s) 73
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
Bsu15I ATCGAT 1 cut(s) 154
BsuTUI ATCGAT 1 cut(s) 154
BtsCI GGATG 1 cut(s) 118
Cfr13I GGNCC 1 cut(s) 304
ClaI ATCGAT 1 cut(s) 154
CsiI ACCWGGT 1 cut(s) 313
Csp6I GTAC 2 cut(s) 248, 367
CviAII CATG 2 cut(s) 90, 203
CviJI RGCY 5 cut(s) 64, 143, 168, 335, 359
CviKI_1 RGCY 5 cut(s) 64, 143, 168, 335, 359
CviQI GTAC 2 cut(s) 248, 367
DdeI CTNAG 2 cut(s) 123, 144
DpnI GATC 4 cut(s) 157, 240, 270, 408
DpnII GATC 4 cut(s) 155, 238, 268, 406
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
Ecl136II GAGCTC 1 cut(s) 359
Eco24I GRGCYC 1 cut(s) 361
Eco47I GGWCC 1 cut(s) 304
Eco53kI GAGCTC 1 cut(s) 359
Eco91I GGTNACC 1 cut(s) 372
EcoICRI GAGCTC 1 cut(s) 359
EcoO65I GGTNACC 1 cut(s) 372
EcoRII CCWGG 2 cut(s) 163, 313
EcoT22I ATGCAT 1 cut(s) 224
EcoT38I GRGCYC 1 cut(s) 361
FaeI CATG 2 cut(s) 93, 206
FaiI YATR 9 cut(s) 8, 34, 59, 91, 183, 204, 224, 302, 418
FatI CATG 2 cut(s) 89, 202
FbaI TGATCA 1 cut(s) 268
Fnu4HI GCNGC 1 cut(s) 62
FokI GGATG 1 cut(s) 105
FriOI GRGCYC 1 cut(s) 361
Fsp4HI GCNGC 1 cut(s) 62
GluI GCNGC 1 cut(s) 62
Hin1II CATG 2 cut(s) 93, 206
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 4 cut(s) 17, 133, 281, 344
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 1 cut(s) 196
Hpy188III TCNNGA 2 cut(s) 122, 362
Hpy8I GTNNAC 1 cut(s) 196
HpyCH4IV ACGT 1 cut(s) 178
HpyCH4V TGCA 3 cut(s) 24, 61, 222
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 408
HpyF3I CTNAG 2 cut(s) 123, 144
HpySE526I ACGT 1 cut(s) 178
Hsp92II CATG 2 cut(s) 93, 206
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 4 cut(s) 155, 238, 268, 406
LpnPI CCDG 7 cut(s) 135, 150, 177, 255, 300, 327, 369
Lsp1109I GCAGC 1 cut(s) 73
LweI GCATC 2 cut(s) 33, 160
MabI ACCWGGT 1 cut(s) 313
MaeII ACGT 1 cut(s) 178
MaeIII GTNAC 1 cut(s) 372
MalI GATC 4 cut(s) 157, 240, 270, 408
MboI GATC 4 cut(s) 155, 238, 268, 406
MboII GAAGA 1 cut(s) 248
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 361
MluCI AATT 1 cut(s) 289
MlyI GAGTC 2 cut(s) 11, 142
MmeI TCCRAC 1 cut(s) 404
MnlI CCTC 1 cut(s) 387
Mph1103I ATGCAT 1 cut(s) 224
MseI TTAA 3 cut(s) 114, 288, 424
MspR9I CCNGG 2 cut(s) 165, 315
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 2 cut(s) 165, 315
MwoI GCNNNNNNNGC 2 cut(s) 70, 408
NdeII GATC 4 cut(s) 155, 238, 268, 406
NlaIII CATG 2 cut(s) 93, 206
NsiI ATGCAT 1 cut(s) 224
NspI RCATGY 1 cut(s) 93
PciI ACATGT 1 cut(s) 89
PctI GAATGC 1 cut(s) 104
PfeI GAWTC 2 cut(s) 281, 344
PflMI CCANNNNNTGG 1 cut(s) 386
PkrI GCNGC 1 cut(s) 63
Ple19I CGATCG 1 cut(s) 158
PleI GAGTC 2 cut(s) 11, 141
PpsI GAGTC 2 cut(s) 11, 141
PscI ACATGT 1 cut(s) 89
Psp124BI GAGCTC 1 cut(s) 361
Psp1406I AACGTT 1 cut(s) 178
Psp6I CCWGG 2 cut(s) 163, 313
PspEI GGTNACC 1 cut(s) 372
PspGI CCWGG 2 cut(s) 163, 313
PspPI GGNCC 1 cut(s) 304
PsuI RGATCY 1 cut(s) 238
PvuI CGATCG 1 cut(s) 158
RsaI GTAC 2 cut(s) 249, 368
RsaNI GTAC 2 cut(s) 248, 367
SacI GAGCTC 1 cut(s) 361
SaqAI TTAA 3 cut(s) 114, 288, 424
SatI GCNGC 1 cut(s) 62
Sau3AI GATC 4 cut(s) 155, 238, 268, 406
Sau96I GGNCC 1 cut(s) 304
SchI GAGTC 2 cut(s) 11, 142
ScrFI CCNGG 2 cut(s) 165, 315
SduI GDGCHC 1 cut(s) 361
SetI ASST 8 cut(s) 39, 66, 145, 166, 181, 319, 361, 379
SexAI ACCWGGT 1 cut(s) 313
SfaNI GCATC 2 cut(s) 33, 160
SfcI CTRYAG 1 cut(s) 352
SinI GGWCC 1 cut(s) 304
SmlI CTYRAG 2 cut(s) 275, 362
SmoI CTYRAG 2 cut(s) 275, 362
Sse9I AATT 1 cut(s) 289
SstI GAGCTC 1 cut(s) 361
StyD4I CCNGG 2 cut(s) 163, 313
TaiI ACGT 1 cut(s) 181
TaqI TCGA 2 cut(s) 154, 158
TaqII GACCGA 1 cut(s) 321
TasI AATT 1 cut(s) 289
TfiI GAWTC 2 cut(s) 281, 344
Tru1I TTAA 3 cut(s) 114, 288, 424
Tru9I TTAA 3 cut(s) 114, 288, 424
TseI GCWGC 1 cut(s) 61
TspDTI ATGAA 4 cut(s) 35, 97, 219, 339
Van91I CCANNNNNTGG 1 cut(s) 386
VpaK11BI GGWCC 1 cut(s) 304
XceI RCATGY 1 cut(s) 93
Zsp2I ATGCAT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.