Rroxscaffold_5G00345980

Lysyl-tRNA synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005056
Physical Location & Seq
Reverse (-)
16569880 .. 16574289
4410 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00345980.1

Sequence Viewer

Length: 1791 bp
ATGGAAAAAGCCGTTGACGAAGCAACGAAGGCCGTCTCGGATTTGGCAATGGAGGGGGAAGGCACTAAGAGCAAAAATGCGCTGAAGAAGGAGCTGAAGAACAAGCAGAAGGAAGAGGAGAGGCGCCGGAAGGAGGAGGAGAAGGCTAAACAGGCTGCTGCGATGGCCTCGCAGCCAAAGAAAAATGCAGCTACTGAAGATGAAGAAATGGACCCAACGCAATATTTTGAAAACAGGCTCAAGTATCTTGCTTCAATAGAAAACGCATATCCTCACAAATTCAATGTTACAATGTCTGATCGAATATTTCTGGAGAAGTTTGAAGGCCTAGAAAATGGGGAGCATAAGGAGGATATCACTGTTTCATTGGCAGGCCGAATCATGAGCAAAAGATCGTCCAGCTCAAAGCTTTTCTTCTATGATTTGCATGCCACTCCTGGTGGTTGGAAGGTCCAAGTTATGGCGGATGCCAGGAAATCGGAGTTGGATGAATCTGAATTTTCTAGGTTCCACTCCAGTGTTAAGCGTGGTGATCTTGTTGGTGTTAGTGGGTTTCCAGTGCTATCACATTGCTTGCATATGATGCCAAGGCAGAAAGCTGGGCCTGTCTCTGATAATGCCAATTTGAAGAAAACTGATGGATGGGTCCCAGGAAGTCCCAGAAATCCTGAAACATACATTCTGAAAGATCAGGAAACTCGTTATCGTCAGCGTTACCTGGATTTAATATTGGCTAGCGAGGTTCGACAAATATTCAAGACCAGATCCAAGATCGTCAAGTACATCAGGAGGTATCTTGACGAACTCGATTTCTTGGAGGTTGAGACTCCAATGATGAACATGATTGCTGGTGGTGCAGCTGCCCGTCCGTTTGTCACTTATCATAATGATCTTAACATGAAGCTTTTCATGCGGATTGCACCCGAACTCTATCTCAAGCAGCTAGTTGTTGGTGGACTGGATCGTGTATATGAGATTGGAAAGCAATTCAGAAATGAGGGTATTGATTTGACACATAATCCTGAGTTCACCACATGTGAATTTTACATGGCCTATGCTGATTACAATGACTTGATGACGATGACCGAGGAAATGTTGAGTGGGATGGTCAAGGAAATTACCGGAGGGTACAAAGTTAAGTACCATTCAAATGGGCTAGATAAGGATCCTATAGAGATTGACTTCACTCCTCCTTTTCGAAGGATCGACATGGTTGAAGAACTAAACAAGATTGCAGGTCTCAACATTAATCCCAAGGATCTTTCCAGTGAGGAAGTTAACAGTTATCTTAAAGAGGCATGCAAGAAGTTTGATATCAAATGTTCTCCTCCTGAAACAACAGCTCGTTTGTTGGACAAGCTTGTGGGCCACTTTTTAGAGGAGACGTGTGTAAATCCAGCTTTCATAATCAACCATCCTGAGATAATGAGTCCGTTAGCCAAGTGGCATAGATCAAAGCCTGGACTGACTGAACGGTTCGAGTTGTTTATCAATAAACATGAAGTTTGCAATGCATACACTGAATTGAATGATCCTGTGGTACAACGCCAACGGTTTGCTGGTCAACTCAAGGATCGACAGTCGGGTGATGATGAAGCAATGGCTTTGGATGAAACATTTTGTACTGCTCTCGAGTATGGGTTACCTCCTACTGGTGGCTGGGGTTTGGGTGTTGACCGCCTATGTATGTTGTTAACTGACTCACAGAATATTAAGGAAGTGCTTCTCTTCCCTGCCATGAAACCACAAGATGATCAACCTCCTGCAAAACCAACAACTGCAGGTGCTTAG

Protein Analysis

596

Amino Acids

67.89

Weight (kDa)

5.86

Isoelectric Point (pI)

51.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_2 PF00152 230 - 580 3.8e-95 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1772
Acc36I ACCTGC 2 cut(s) 1226, 1772
AccB1I GGYRCC 1 cut(s) 123
AccB7I CCANNNNNTGG 1 cut(s) 460
AciI CCGC 3 cut(s) 464, 913, 1678
AclWI GGATC 8 cut(s) 759, 969, 1160, 1173, 1211, 1266, 1526, 1581
AcsI RAATTY 3 cut(s) 278, 497, 1040
AcuI CTGAAG 3 cut(s) 104, 116, 216
AcyI GRCGYC 1 cut(s) 124
AfaI GTAC 5 cut(s) 782, 1130, 1142, 1542, 1624
AfiI CCNNNNNNNGG 4 cut(s) 133, 460, 1652, 1655
AflIII ACRYGT 2 cut(s) 1034, 1385
AgsI TTSAA 9 cut(s) 230, 255, 283, 323, 628, 757, 1149, 1217, 1528
AjiI CACGTC 1 cut(s) 1386
AjnI CCWGG 5 cut(s) 436, 470, 649, 717, 1459
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
AleI CACNNNNGTG 1 cut(s) 516
Alw26I GTCTC 5 cut(s) 40, 613, 818, 1244, 1376
AlwI GGATC 8 cut(s) 759, 969, 1160, 1173, 1211, 1266, 1526, 1581
AlwNI CAGNNNCTG 1 cut(s) 194
Ama87I CYCGRG 1 cut(s) 1631
AoxI GGCC 7 cut(s) 30, 165, 325, 373, 602, 1050, 1366
ApeKI GCWGC 7 cut(s) 155, 158, 172, 188, 857, 860, 940
ApoI RAATTY 3 cut(s) 278, 497, 1040
AseI ATTAAT 1 cut(s) 1248
Asp700I GAANNNNTTC 2 cut(s) 905, 1722
AspLEI GCGC 2 cut(s) 82, 126
AspS9I GGNCC 5 cut(s) 211, 451, 602, 646, 1366
AsuHPI GGTGA 3 cut(s) 542, 1021, 1598
AsuII TTCGAA 1 cut(s) 1198
AsuNHI GCTAGC 1 cut(s) 734
AvaI CYCGRG 1 cut(s) 1631
AvaII GGWCC 3 cut(s) 211, 451, 646
BamHI GGATCC 1 cut(s) 1165
BanI GGYRCC 1 cut(s) 123
BbvI GCAGC 7 cut(s) 142, 145, 184, 200, 847, 869, 952
BccI CCATC 5 cut(s) 157, 632, 636, 1099, 1422
BceAI ACGGC 1 cut(s) 17
BciT130I CCWGG 5 cut(s) 438, 472, 651, 719, 1461
BclI TGATCA 1 cut(s) 1753
BcoDI GTCTC 5 cut(s) 40, 613, 818, 1244, 1376
BfaI CTAG 5 cut(s) 329, 504, 735, 944, 1157
BfmI CTRYAG 2 cut(s) 1170, 1779
BfoI RGCGCY 1 cut(s) 127
BfuAI ACCTGC 2 cut(s) 1226, 1772
BisI GCNGC 7 cut(s) 156, 159, 173, 189, 858, 861, 941
BlsI GCNGC 7 cut(s) 157, 160, 174, 190, 859, 862, 942
Bme1390I CCNGG 5 cut(s) 438, 472, 651, 719, 1461
Bme18I GGWCC 3 cut(s) 211, 451, 646
BmeT110I CYCGRG 1 cut(s) 1631
BmgBI CACGTC 1 cut(s) 1386
BmgT120I GGNCC 5 cut(s) 211, 451, 602, 646, 1366
BmiI GGNNCC 6 cut(s) 125, 213, 509, 647, 648, 1167
BmrFI CCNGG 5 cut(s) 438, 472, 651, 719, 1461
BmsI GCATC 2 cut(s) 457, 573
BmtI GCTAGC 1 cut(s) 738
BpmI CTGGAG 2 cut(s) 332, 499
Bpu14I TTCGAA 1 cut(s) 1198
BpuEI CTTGAG 3 cut(s) 224, 920, 1553
BsaBI GATNNNNATC 1 cut(s) 1164
BsaHI GRCGYC 1 cut(s) 124
BsaI GGTCTC 1 cut(s) 1244
BsaJI CCNNGG 4 cut(s) 587, 649, 1086, 1254
BsaWI WCCGGW 1 cut(s) 1121
Bsc4I CCNNNNNNNGG 4 cut(s) 133, 460, 1652, 1655
Bse1I ACTGG 5 cut(s) 516, 557, 963, 1266, 1657
Bse3DI GCAATG 4 cut(s) 54, 568, 1516, 1605
Bse8I GATNNNNATC 1 cut(s) 1164
BseBI CCWGG 5 cut(s) 438, 472, 651, 719, 1461
BseDI CCNNGG 4 cut(s) 587, 649, 1086, 1254
BseGI GGATG 6 cut(s) 472, 493, 647, 1110, 1414, 1615
BseJI GATNNNNATC 1 cut(s) 1164
BseLI CCNNNNNNNGG 4 cut(s) 133, 460, 1652, 1655
BseMI GCAATG 4 cut(s) 54, 568, 1516, 1605
BseMII CTCAG 2 cut(s) 1014, 1410
BseNI ACTGG 5 cut(s) 516, 557, 963, 1266, 1657
BseRI GAGGAG 6 cut(s) 131, 149, 152, 1179, 1317, 1394
BseXI GCAGC 7 cut(s) 142, 145, 184, 200, 847, 869, 952
BseYI CCCAGC 2 cut(s) 599, 1659
BsgI GTGCAG 1 cut(s) 876
BshFI GGCC 7 cut(s) 32, 167, 327, 375, 604, 1052, 1368
BshNI GGYRCC 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 1631
BsiSI CCGG 2 cut(s) 127, 1122
BslFI GGGAC 2 cut(s) 632, 642
BslI CCNNNNNNNGG 4 cut(s) 133, 460, 1652, 1655
BsmAI GTCTC 5 cut(s) 40, 613, 818, 1244, 1376
BsmBI CGTCTC 2 cut(s) 40, 1376
BsmFI GGGAC 2 cut(s) 632, 642
BsnI GGCC 7 cut(s) 32, 167, 327, 375, 604, 1052, 1368
Bso31I GGTCTC 1 cut(s) 1244
BsoBI CYCGRG 1 cut(s) 1631
Bsp119I TTCGAA 1 cut(s) 1198
BspACI CCGC 3 cut(s) 464, 913, 1678
BspANI GGCC 7 cut(s) 32, 167, 327, 375, 604, 1052, 1368
BspCNI CTCAG 2 cut(s) 1015, 1411
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 6 cut(s) 125, 213, 509, 647, 648, 1167
BspMAI CTGCAG 1 cut(s) 1783
BspMI ACCTGC 2 cut(s) 1226, 1772
BspOI GCTAGC 1 cut(s) 738
BspPI GGATC 8 cut(s) 759, 969, 1160, 1173, 1211, 1266, 1526, 1581
BspT104I TTCGAA 1 cut(s) 1198
BspT107I GGYRCC 1 cut(s) 123
BspTNI GGTCTC 1 cut(s) 1244
BsrDI GCAATG 4 cut(s) 54, 568, 1516, 1605
BsrI ACTGG 5 cut(s) 516, 557, 963, 1266, 1657
BssECI CCNNGG 4 cut(s) 587, 649, 1086, 1254
BssNI GRCGYC 1 cut(s) 124
BssT1I CCWWGG 2 cut(s) 587, 1254
Bst2UI CCWGG 5 cut(s) 438, 472, 651, 719, 1461
Bst4CI ACNGT 5 cut(s) 361, 1283, 1476, 1554, 1581
Bst6I CTCTTC 2 cut(s) 108, 1733
BstACI GRCGYC 1 cut(s) 124
BstAPI GCANNNNNTGC 1 cut(s) 583
BstBI TTCGAA 1 cut(s) 1198
BstC8I GCNNGC 5 cut(s) 373, 429, 575, 736, 1300
BstDEI CTNAG 4 cut(s) 66, 1023, 1419, 1788
BstEII GGTNACC 1 cut(s) 1641
BstF5I GGATG 6 cut(s) 472, 493, 647, 1110, 1414, 1615
BstH2I RGCGCY 1 cut(s) 127
BstHHI GCGC 2 cut(s) 82, 126
BstMAI GTCTC 5 cut(s) 40, 613, 818, 1244, 1376
BstMWI GCNNNNNNNGC 7 cut(s) 29, 69, 152, 164, 583, 854, 910
BstNI CCWGG 5 cut(s) 438, 472, 651, 719, 1461
BstNSI RCATGY 3 cut(s) 431, 1038, 1302
BstPI GGTNACC 1 cut(s) 1641
BstSCI CCNGG 5 cut(s) 436, 470, 649, 717, 1459
BstSFI CTRYAG 2 cut(s) 1170, 1779
BstV1I GCAGC 7 cut(s) 142, 145, 184, 200, 847, 869, 952
BstX2I RGATCY 3 cut(s) 764, 1165, 1258
BstXI CCANNNNNNTGG 1 cut(s) 1151
BstYI RGATCY 3 cut(s) 764, 1165, 1258
BsuRI GGCC 7 cut(s) 32, 167, 327, 375, 604, 1052, 1368
BtgZI GCGATG 1 cut(s) 176
BtrI CACGTC 1 cut(s) 1386
BtsCI GGATG 6 cut(s) 472, 493, 647, 1110, 1414, 1615
BtsIMutI CAGTG 5 cut(s) 357, 523, 564, 1273, 1518
BveI ACCTGC 2 cut(s) 1226, 1772
Cac8I GCNNGC 5 cut(s) 373, 429, 575, 736, 1300
CaiI CAGNNNCTG 1 cut(s) 194
CciI TCATGA 1 cut(s) 381
CfoI GCGC 2 cut(s) 82, 126
Cfr13I GGNCC 5 cut(s) 211, 451, 602, 646, 1366
Csp6I GTAC 5 cut(s) 781, 1129, 1141, 1541, 1623
CviQI GTAC 5 cut(s) 781, 1129, 1141, 1541, 1623
DdeI CTNAG 4 cut(s) 66, 1023, 1419, 1788
DinI GGCGCC 1 cut(s) 125
Eam1104I CTCTTC 2 cut(s) 108, 1733
EarI CTCTTC 2 cut(s) 108, 1733
EciI GGCGGA 1 cut(s) 479
Eco130I CCWWGG 2 cut(s) 587, 1254
Eco147I AGGCCT 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 1244
Eco32I GATATC 2 cut(s) 355, 1315
Eco47I GGWCC 3 cut(s) 211, 451, 646
Eco57I CTGAAG 3 cut(s) 104, 116, 216
Eco88I CYCGRG 1 cut(s) 1631
Eco91I GGTNACC 1 cut(s) 1641
EcoO109I RGGNCCY 1 cut(s) 646
EcoO65I GGTNACC 1 cut(s) 1641
EcoRII CCWGG 5 cut(s) 436, 470, 649, 717, 1459
EcoRV GATATC 2 cut(s) 355, 1315
EcoT14I CCWWGG 2 cut(s) 587, 1254
EcoT22I ATGCAT 1 cut(s) 1516
EgeI GGCGCC 1 cut(s) 125
EheI GGCGCC 1 cut(s) 125
ErhI CCWWGG 2 cut(s) 587, 1254
Esp3I CGTCTC 2 cut(s) 40, 1376
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FaqI GGGAC 2 cut(s) 632, 642
FauNDI CATATG 1 cut(s) 579
FbaI TGATCA 1 cut(s) 1753
Fnu4HI GCNGC 7 cut(s) 156, 159, 173, 189, 858, 861, 941
FokI GGATG 6 cut(s) 479, 500, 654, 1117, 1401, 1622
Fsp4HI GCNGC 7 cut(s) 156, 159, 173, 189, 858, 861, 941
FspBI CTAG 5 cut(s) 329, 504, 735, 944, 1157
GlaI GCGC 2 cut(s) 81, 125
GluI GCNGC 7 cut(s) 156, 159, 173, 189, 858, 861, 941
GsaI CCCAGC 2 cut(s) 603, 1663
GsuI CTGGAG 2 cut(s) 332, 499
HaeII RGCGCY 1 cut(s) 127
HaeIII GGCC 7 cut(s) 32, 167, 327, 375, 604, 1052, 1368
HapII CCGG 2 cut(s) 127, 1122
HhaI GCGC 2 cut(s) 82, 126
Hin1I GRCGYC 1 cut(s) 124
Hin6I GCGC 2 cut(s) 80, 124
HinP1I GCGC 2 cut(s) 80, 124
HincII GTYRAC 5 cut(s) 16, 1279, 1565, 1675, 1695
HindII GTYRAC 5 cut(s) 16, 1279, 1565, 1675, 1695
HindIII AAGCTT 3 cut(s) 407, 902, 1358
HinfI GANTC 5 cut(s) 378, 491, 826, 1429, 1700
HpaI GTTAAC 2 cut(s) 1279, 1695
HpaII CCGG 2 cut(s) 127, 1122
HphI GGTGA 3 cut(s) 542, 1021, 1598
Hpy166II GTNNAC 7 cut(s) 16, 956, 1029, 1279, 1565, 1675, 1695
Hpy188I TCNGA 7 cut(s) 40, 298, 481, 496, 613, 684, 992
Hpy8I GTNNAC 7 cut(s) 16, 956, 1029, 1279, 1565, 1675, 1695
HpyAV CCTTC 9 cut(s) 22, 53, 82, 103, 124, 136, 317, 442, 1194
HpyCH4III ACNGT 5 cut(s) 361, 1283, 1476, 1554, 1581
HpyCH4IV ACGT 1 cut(s) 1385
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 69, 152, 164, 583, 854, 910
HpyF3I CTNAG 4 cut(s) 66, 1023, 1419, 1788
HpySE526I ACGT 1 cut(s) 1385
Hsp92I GRCGYC 1 cut(s) 124
HspAI GCGC 2 cut(s) 80, 124
KasI GGCGCC 1 cut(s) 123
KflI GGGWCCC 1 cut(s) 646
Ksp22I TGATCA 1 cut(s) 1753
KspAI GTTAAC 2 cut(s) 1279, 1695
LmnI GCTCC 2 cut(s) 91, 340
Lsp1109I GCAGC 7 cut(s) 142, 145, 184, 200, 847, 869, 952
LweI GCATC 2 cut(s) 457, 573
MaeI CTAG 5 cut(s) 329, 504, 735, 944, 1157
MaeII ACGT 1 cut(s) 1385
MaeIII GTNAC 4 cut(s) 286, 713, 874, 1641
MboII GAAGA 9 cut(s) 97, 109, 125, 209, 215, 406, 640, 1229, 1720
MflI RGATCY 3 cut(s) 764, 1165, 1258
MluCI AATT 7 cut(s) 278, 497, 622, 986, 1040, 1116, 1523
Mly113I GGCGCC 1 cut(s) 124
MlyI GAGTC 3 cut(s) 820, 1438, 1694
MmeI TCCRAC 3 cut(s) 425, 465, 1332
Mph1103I ATGCAT 1 cut(s) 1516
MroXI GAANNNNTTC 2 cut(s) 905, 1722
MseI TTAA 9 cut(s) 522, 725, 894, 1137, 1248, 1278, 1290, 1694, 1713
MslI CAYNNNNRTG 2 cut(s) 516, 1149
MspA1I CMGCKG 1 cut(s) 860
MspI CCGG 2 cut(s) 127, 1122
MspR9I CCNGG 5 cut(s) 438, 472, 651, 719, 1461
MvaI CCWGG 5 cut(s) 438, 472, 651, 719, 1461
MwoI GCNNNNNNNGC 7 cut(s) 29, 69, 152, 164, 583, 854, 910
NarI GGCGCC 1 cut(s) 124
NdeI CATATG 1 cut(s) 579
NheI GCTAGC 1 cut(s) 734
NlaIV GGNNCC 6 cut(s) 125, 213, 509, 647, 648, 1167
NmuCI GTSAC 1 cut(s) 874
NsiI ATGCAT 1 cut(s) 1516
NspI RCATGY 3 cut(s) 431, 1038, 1302
NspV TTCGAA 1 cut(s) 1198
OliI CACNNNNGTG 1 cut(s) 516
PaeI GCATGC 2 cut(s) 431, 1302
PaeR7I CTCGAG 1 cut(s) 1631
PagI TCATGA 1 cut(s) 381
PaqCI CACCTGC 1 cut(s) 1772
PceI AGGCCT 1 cut(s) 327
PciI ACATGT 1 cut(s) 1034
PdmI GAANNNNTTC 2 cut(s) 905, 1722
PfeI GAWTC 2 cut(s) 378, 491
PflMI CCANNNNNTGG 1 cut(s) 460
PkrI GCNGC 7 cut(s) 157, 160, 174, 190, 859, 862, 942
PleI GAGTC 3 cut(s) 820, 1437, 1694
PluTI GGCGCC 1 cut(s) 127
PpsI GAGTC 3 cut(s) 820, 1437, 1694
PpuMI RGGWCCY 1 cut(s) 646
PscI ACATGT 1 cut(s) 1034
PshBI ATTAAT 1 cut(s) 1248
Psp5II RGGWCCY 1 cut(s) 646
Psp6I CCWGG 5 cut(s) 436, 470, 649, 717, 1459
PspEI GGTNACC 1 cut(s) 1641
PspFI CCCAGC 2 cut(s) 599, 1659
PspGI CCWGG 5 cut(s) 436, 470, 649, 717, 1459
PspN4I GGNNCC 6 cut(s) 125, 213, 509, 647, 648, 1167
PspPI GGNCC 5 cut(s) 211, 451, 602, 646, 1366
PspPPI RGGWCCY 1 cut(s) 646
PstI CTGCAG 1 cut(s) 1783
PstNI CAGNNNCTG 1 cut(s) 194
PsuI RGATCY 3 cut(s) 764, 1165, 1258
PvuII CAGCTG 1 cut(s) 860
RsaI GTAC 5 cut(s) 782, 1130, 1142, 1542, 1624
RsaNI GTAC 5 cut(s) 781, 1129, 1141, 1541, 1623
RseI CAYNNNNRTG 2 cut(s) 516, 1149
SaqAI TTAA 9 cut(s) 522, 725, 894, 1137, 1248, 1278, 1290, 1694, 1713
SatI GCNGC 7 cut(s) 156, 159, 173, 189, 858, 861, 941
Sau96I GGNCC 5 cut(s) 211, 451, 602, 646, 1366
SchI GAGTC 3 cut(s) 820, 1438, 1694
ScrFI CCNGG 5 cut(s) 438, 472, 651, 719, 1461
SfaNI GCATC 2 cut(s) 457, 573
SfcI CTRYAG 2 cut(s) 1170, 1779
SfoI GGCGCC 1 cut(s) 125
Sfr274I CTCGAG 1 cut(s) 1631
SfuI TTCGAA 1 cut(s) 1198
SinI GGWCC 3 cut(s) 211, 451, 646
SlaI CTCGAG 1 cut(s) 1631
SmiMI CAYNNNNRTG 2 cut(s) 516, 1149
SmlI CTYRAG 4 cut(s) 239, 935, 1568, 1631
SmoI CTYRAG 4 cut(s) 239, 935, 1568, 1631
SphI GCATGC 2 cut(s) 431, 1302
Sse9I AATT 7 cut(s) 278, 497, 622, 986, 1040, 1116, 1523
SseBI AGGCCT 1 cut(s) 327
SsiI CCGC 3 cut(s) 464, 913, 1678
SspDI GGCGCC 1 cut(s) 123
SspI AATATT 5 cut(s) 224, 306, 729, 753, 1711
SspMI CTAG 5 cut(s) 329, 504, 735, 944, 1157
StuI AGGCCT 1 cut(s) 327
StyD4I CCNGG 5 cut(s) 436, 470, 649, 717, 1459
StyI CCWWGG 2 cut(s) 587, 1254
TaaI ACNGT 5 cut(s) 361, 1283, 1476, 1554, 1581
TaiI ACGT 1 cut(s) 1388
TaqI TCGA 8 cut(s) 301, 745, 807, 1198, 1206, 1479, 1576, 1632
TaqII GACCGA 1 cut(s) 1100
TasI AATT 7 cut(s) 278, 497, 622, 986, 1040, 1116, 1523
TatI WGTACW 2 cut(s) 780, 1622
TfiI GAWTC 2 cut(s) 378, 491
Tru1I TTAA 9 cut(s) 522, 725, 894, 1137, 1248, 1278, 1290, 1694, 1713
Tru9I TTAA 9 cut(s) 522, 725, 894, 1137, 1248, 1278, 1290, 1694, 1713
TscAI CASTG 5 cut(s) 364, 523, 564, 1273, 1525
TseFI GTSAC 1 cut(s) 874
TseI GCWGC 7 cut(s) 155, 158, 172, 188, 857, 860, 940
Tsp45I GTSAC 1 cut(s) 874
TspGWI ACGGA 2 cut(s) 858, 1422
TspRI CASTG 5 cut(s) 364, 523, 564, 1273, 1525
Van91I CCANNNNNTGG 1 cut(s) 460
VpaK11BI GGWCC 3 cut(s) 211, 451, 646
VspI ATTAAT 1 cut(s) 1248
XapI RAATTY 3 cut(s) 278, 497, 1040
XceI RCATGY 3 cut(s) 431, 1038, 1302
XcmI CCANNNNNNNNNTGG 1 cut(s) 1556
XhoI CTCGAG 1 cut(s) 1631
XmnI GAANNNNTTC 2 cut(s) 905, 1722
XspI CTAG 5 cut(s) 329, 504, 735, 944, 1157
Zsp2I ATGCAT 1 cut(s) 1516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.