Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005056

Physical Location & Seq

Genomic Coordinates Reverse (-)

29343851 .. 29350697

Gene Structure 6847 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1255000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGGCAGGCACACCCGAAAAAACGGCGAAAGCGGTCGCAGATTTGACATTGGAATCGGAAGGAGCTATGAGCAAAAATGCACGGAAGAAGGAGTTGAAGAACAAGCAAAGGGAAGAAGAAAGGCGCCGGAAAGAGGAGGAGAAGGCCAAGCAGGCTGCTGCCGTGGCAAGCTCGCAAGTTAAGAAAGATGTAGCAACTGAAGATGACGAAATGGATCCAACGCAATACTATGAAAACAGACTGAAGTATCTTGCTACCCAGAAGGTAGATAAAAATCCATATCCTCACAAATTCTCTTGTTCAATCACCGTGTGCGAATGTGTAGAGAAGTATGCAGGCTTAAGCGATGGGGAGCATCTTGAGGATATCAGTGTTTCATTGGCTGGGCGAATCATGAGTAAAAGATCGTCTAGCTCAAAACTTTTCTTTTATGACTTACATGGTGGAGGACCTGATGGTGAGAAGGTCCAAGTGATGGCTGATTTAAGAAAATCAGAGCTGGATGAATCTGAATTTTCTAAGTTCCATTCTAGTGTTAAGCGTGGTGATATTGTTGGTATTACTGGGTTTCCAGGAAAGACTAAGAGGGGGGAGTTGAGCATTTTCCCAAGATCATTTATAGTGTTATCACATTGTCTTCATATGATGCCAAGGCAGAAACCTGGTCCTGCTTCTGATAGTGCTAATGTGAAGAAAACTGATCGATGGGTCCCAGGAAGTCCCAGGAATCCTGAAGCATACATTTTAAAAGACCAGGAAACTCGCTATCGTCAACGTTATTTGGATCTAATATTGACTAATGAGGTTCGACAAATATTCAAGACCAGATTTAAGGTCATTCAATATATTAGAAGGTTCCTGGACAATCTGGACTTCTTAGAGGTCGAAACTCCTATGATGAATATGATTGCTGGTGGAGCGGCTGCCCGTCCTTTTGTGACTTATCACAATGAACTTGACATGAAGCTTTTCATGCGCATTGCACCTGAACTCTATCTTAAACAGCTAGTTGTTGGTGGCCTGGATCGTGTTTATGAGATTGGAAAACAATTTAGAAATGAGGGCATTGATTTGACTCATAATCCTGAGTTCACTACCTGTGAATTCTATATGGCTTATGCAGACTACAATGACTTGATGACACTGACTGAGGAAATGTTGAGTGGGATGGTCAAGGAACTAACTGGTGGATATAAAATCAAGTATCATTCGAATGGGCTCGACAAAGATCCAATTGAGATTGACTTTACTCCTCCTTTCAGGATCGACATGGTTGAGGAATTAAATAAGATTGCAGGTCTCAACATAAATCCGGAGAATCTCTCCAGTGCAGAAGCTAACCAATATCTTAAGGATGCGTGCAAGAAATTTGATATCAAATGTTCCCCTCCTGAAACAACAACTCGTTTGTTGGACAAGCTTGTGGGGCACTTTTTGGAAGAGACGTGTGTAAATCCCGCGTTCATCATTAACCATCCTGAGATAATGAGTCCTTTAGCAAAGTGGCATCGATCAAAACCTGGGCTGACTGAACGTTTTGAATTGTTTGTCAACAAGCATGAACTTGCCAATGCATACACAGAATTGAATGATCCTGTGGTACAACGCCAACGATTTGCTGATCAACTCAAGGACCGACAATCAGGTGATGATGAAGCAATGGCACTGGACGAAACATTTTGCAGAGCTCTTGAGTATGGGTTACCTCCAACTGGTGGTTGGGGTTTGGGCGTTGACCGGCTCTGTATGTGGTTAACTGATTCACAGAATATTAAGGAAGTTCTACTCTTCCCGGCTATGAAACCACAGGATGAGCCTTCTGCTAAAGCCATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0009506 GO:0016874 GO:0016875 GO:0030054 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0140098 GO:0140101

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2 tRNA_anti-codon

Protein Analysis

612

Amino Acids

69.6

Weight (kDa)

6.01

Isoelectric Point (pI)

48.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_anti-codon	PF01336	125 - 209	1.1e-10	OB-fold nucleic acid binding domain
tRNA-synt_2	PF00152	251 - 600	1.8e-92	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005056)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11710 AT3G30805
fragaria_vesca	FvH4_2g08732 FvH4_4g07860 FvH4_4g07860 FvH4_4g07860 FvH4_6g23270
malus_domestica	MD03G1170800.v1.1 MD13G1247500.v1.1 MD13G1247600.v1.1 MD13G1248200.v1.1 MD16G1255000.v1.1
prunus_persica	Prupe.1G082400_v2.0.a1 Prupe.1G082400_v2.0.a1 Prupe.1G083700_v2.0.a1
pyrus_communis	pycom03g12770 pycom13g21870 pycom13g21930 pycom16g21650
rosa_chinensis	RchiOBHm_Chr4g0401131
rosa_laevigata	RLG00000009161
rosa_multiflora	Rmu_sc0001909.1_g000026 Rmu_sc0037183.1_g000001
rosa_roxburghii	Rroxscaffold_5G00345980
rosa_rugosa	Rorug02G0525300 Rorug04G0028100
rosa_samantha	Rh2DG614600 Rh4BG100600 Rh4DG097900
rosa_wichuraiana	Rw4G008540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	977
Acc36I	ACCTGC	1 cut(s)	1286
AccB1I	GGYRCC	1 cut(s)	123
AccB7I	CCANNNNNTGG	2 cut(s)	475, 1715
AccBSI	CCGCTC	1 cut(s)	920
AccII	CGCG	1 cut(s)	1460
AccIII	TCCGGA	1 cut(s)	1312
AciI	CCGC	3 cut(s)	32, 920, 1458
AclI	AACGTT	2 cut(s)	775, 1534
AclWI	GGATC	7 cut(s)	209, 222, 792, 1032, 1223, 1271, 1586
AcsI	RAATTY	4 cut(s)	290, 512, 1103, 1367
AcuI	CTGAAG	3 cut(s)	219, 263, 753
AcyI	GRCGYC	1 cut(s)	124
AdeI	CACNNNGTG	1 cut(s)	312
AfaI	GTAC	1 cut(s)	1602
AfiI	CCNNNNNNNGG	4 cut(s)	133, 475, 1712, 1715
AflII	CTTAAG	2 cut(s)	340, 1349
AflIII	ACRYGT	1 cut(s)	1445
AgsI	TTSAA	6 cut(s)	97, 303, 820, 842, 1541, 1588
AjiI	CACGTC	1 cut(s)	1446
AjnI	CCWGG	8 cut(s)	571, 661, 712, 722, 753, 858, 1020, 1519
AluBI	AGCT	9 cut(s)	65, 171, 414, 499, 967, 1006, 1337, 1420, 1688
AluI	AGCT	9 cut(s)	65, 171, 414, 499, 967, 1006, 1337, 1420, 1688
Alw21I	GWGCWC	1 cut(s)	1690
Alw26I	GTCTC	2 cut(s)	1304, 1436
AlwI	GGATC	7 cut(s)	209, 222, 792, 1032, 1223, 1271, 1586
Aor13HI	TCCGGA	1 cut(s)	1312
AoxI	GGCC	2 cut(s)	144, 1018
ApeKI	GCWGC	3 cut(s)	155, 158, 923
ApoI	RAATTY	4 cut(s)	290, 512, 1103, 1367
Asp700I	GAANNNNTTC	1 cut(s)	968
AspLEI	GCGC	2 cut(s)	126, 978
AspS9I	GGNCC	5 cut(s)	449, 466, 665, 709, 1633
AsuC2I	CCSGG	1 cut(s)	1793
AsuHPI	GGTGA	4 cut(s)	298, 470, 557, 1658
AsuII	TTCGAA	1 cut(s)	1211
AvaII	GGWCC	5 cut(s)	449, 466, 665, 709, 1633
BaeGI	GKGCMC	1 cut(s)	1431
BaeI	ACNNNNGTAYC	2 cut(s)	230, 263
BamHI	GGATCC	1 cut(s)	214
BanI	GGYRCC	1 cut(s)	123
BanII	GRGCYC	2 cut(s)	1221, 1690
BbsI	GAAGAC	1 cut(s)	629
Bbv12I	GWGCWC	1 cut(s)	1690
BbvI	GCAGC	3 cut(s)	142, 145, 910
BccI	CCATC	6 cut(s)	341, 449, 469, 699, 1162, 1482
BceAI	ACGGC	2 cut(s)	39, 146
BciT130I	CCWGG	8 cut(s)	573, 663, 714, 724, 755, 860, 1022, 1521
BclI	TGATCA	1 cut(s)	1621
BcnI	CCSGG	1 cut(s)	1793
BcoDI	GTCTC	2 cut(s)	1304, 1436
BfaI	CTAG	3 cut(s)	411, 531, 1007
BfoI	RGCGCY	1 cut(s)	127
BfrI	CTTAAG	2 cut(s)	340, 1349
BfuAI	ACCTGC	1 cut(s)	1286
BglI	GCCNNNNNGGC	1 cut(s)	152
BisI	GCNGC	4 cut(s)	156, 159, 921, 924
BlsI	GCNGC	4 cut(s)	157, 160, 922, 925
Bme1390I	CCNGG	9 cut(s)	573, 663, 714, 724, 755, 860, 1022, 1521, 1793
Bme18I	GGWCC	5 cut(s)	449, 466, 665, 709, 1633
BmgBI	CACGTC	1 cut(s)	1446
BmgT120I	GGNCC	5 cut(s)	449, 466, 665, 709, 1633
BmiI	GGNNCC	5 cut(s)	125, 216, 710, 711, 857
BmrFI	CCNGG	9 cut(s)	573, 663, 714, 724, 755, 860, 1022, 1521, 1793
BmrI	ACTGGG	1 cut(s)	573
BmsI	GCATC	4 cut(s)	364, 636, 1345, 1516
BmuI	ACTGGG	1 cut(s)	573
BpiI	GAAGAC	1 cut(s)	629
BplI	GAGNNNNNCTC	2 cut(s)	1307, 1339
BpmI	CTGGAG	1 cut(s)	1309
Bpu14I	TTCGAA	1 cut(s)	1211
BpuEI	CTTGAG	3 cut(s)	380, 1613, 1712
BpuMI	CCSGG	1 cut(s)	1793
Bsa29I	ATCGAT	2 cut(s)	703, 1510
BsaBI	GATNNNNATC	1 cut(s)	273
BsaHI	GRCGYC	1 cut(s)	124
BsaI	GGTCTC	1 cut(s)	1304
BsaJI	CCNNGG	5 cut(s)	162, 650, 712, 722, 1520
BsaWI	WCCGGW	1 cut(s)	1312
Bsc4I	CCNNNNNNNGG	4 cut(s)	133, 475, 1712, 1715
Bse118I	RCCGGY	1 cut(s)	1737
Bse1I	ACTGG	5 cut(s)	568, 1189, 1326, 1671, 1717
Bse3DI	GCAATG	2 cut(s)	978, 1665
Bse8I	GATNNNNATC	1 cut(s)	273
BseAI	TCCGGA	1 cut(s)	1312
BseBI	CCWGG	8 cut(s)	573, 663, 714, 724, 755, 860, 1022, 1521
BseCI	ATCGAT	2 cut(s)	703, 1510
BseDI	CCNNGG	5 cut(s)	162, 650, 712, 722, 1520
BseGI	GGATG	5 cut(s)	508, 1173, 1360, 1474, 1816
BseJI	GATNNNNATC	1 cut(s)	273
BseLI	CCNNNNNNNGG	4 cut(s)	133, 475, 1712, 1715
BseMI	GCAATG	2 cut(s)	978, 1665
BseMII	CTCAG	3 cut(s)	1077, 1140, 1470
BseNI	ACTGG	5 cut(s)	568, 1189, 1326, 1671, 1717
BseRI	GAGGAG	3 cut(s)	149, 152, 1242
BseSI	GKGCMC	1 cut(s)	1431
BseXI	GCAGC	3 cut(s)	142, 145, 910
BseYI	CCCAGC	1 cut(s)	383
BsgI	GTGCAG	1 cut(s)	1350
Bsh1236I	CGCG	1 cut(s)	1460
Bsh1285I	CGRYCG	1 cut(s)	36
BshFI	GGCC	2 cut(s)	146, 1020
BshNI	GGYRCC	1 cut(s)	123
BshVI	ATCGAT	2 cut(s)	703, 1510
BsiEI	CGRYCG	1 cut(s)	36
BsiHKAI	GWGCWC	1 cut(s)	1690
BsiSI	CCGG	4 cut(s)	127, 1313, 1738, 1793
BslFI	GGGAC	2 cut(s)	695, 705
BslI	CCNNNNNNNGG	4 cut(s)	133, 475, 1712, 1715
BsmAI	GTCTC	2 cut(s)	1304, 1436
BsmBI	CGTCTC	1 cut(s)	1436
BsmFI	GGGAC	2 cut(s)	695, 705
BsnI	GGCC	2 cut(s)	146, 1020
Bso31I	GGTCTC	1 cut(s)	1304
Bsp119I	TTCGAA	1 cut(s)	1211
Bsp1286I	GDGCHC	3 cut(s)	1221, 1431, 1690
Bsp13I	TCCGGA	1 cut(s)	1312
BspACI	CCGC	3 cut(s)	32, 920, 1458
BspANI	GGCC	2 cut(s)	146, 1020
BspCNI	CTCAG	3 cut(s)	1078, 1141, 1471
BspDI	ATCGAT	2 cut(s)	703, 1510
BspEI	TCCGGA	1 cut(s)	1312
BspFNI	CGCG	1 cut(s)	1460
BspHI	TCATGA	1 cut(s)	393
BspLI	GGNNCC	5 cut(s)	125, 216, 710, 711, 857
BspMI	ACCTGC	1 cut(s)	1286
BspPI	GGATC	7 cut(s)	209, 222, 792, 1032, 1223, 1271, 1586
BspT104I	TTCGAA	1 cut(s)	1211
BspT107I	GGYRCC	1 cut(s)	123
BspTI	CTTAAG	2 cut(s)	340, 1349
BspTNI	GGTCTC	1 cut(s)	1304
BsrBI	CCGCTC	1 cut(s)	920
BsrDI	GCAATG	2 cut(s)	978, 1665
BsrFI	RCCGGY	1 cut(s)	1737
BsrI	ACTGG	5 cut(s)	568, 1189, 1326, 1671, 1717
BssAI	RCCGGY	1 cut(s)	1737
BssECI	CCNNGG	5 cut(s)	162, 650, 712, 722, 1520
BssNI	GRCGYC	1 cut(s)	124
BssT1I	CCWWGG	1 cut(s)	650
Bst2UI	CCWGG	8 cut(s)	573, 663, 714, 724, 755, 860, 1022, 1521
Bst4CI	ACNGT	1 cut(s)	310
Bst6I	CTCTTC	2 cut(s)	1434, 1793
BstACI	GRCGYC	1 cut(s)	124
BstAFI	CTTAAG	2 cut(s)	340, 1349
BstBI	TTCGAA	1 cut(s)	1211
BstC8I	GCNNGC	6 cut(s)	7, 153, 169, 173, 337, 1360
BstDEI	CTNAG	6 cut(s)	519, 582, 877, 1086, 1149, 1479
BstDSI	CCRYGG	1 cut(s)	162
BstEII	GGTNACC	1 cut(s)	1701
BstF5I	GGATG	5 cut(s)	508, 1173, 1360, 1474, 1816
BstFNI	CGCG	1 cut(s)	1460
BstH2I	RGCGCY	1 cut(s)	127
BstHHI	GCGC	2 cut(s)	126, 978
BstMAI	GTCTC	2 cut(s)	1304, 1436
BstMCI	CGRYCG	1 cut(s)	36
BstMWI	GCNNNNNNNGC	5 cut(s)	152, 164, 917, 973, 1426
BstNI	CCWGG	8 cut(s)	573, 663, 714, 724, 755, 860, 1022, 1521
BstPI	GGTNACC	1 cut(s)	1701
BstSCI	CCNGG	9 cut(s)	571, 661, 712, 722, 753, 858, 1020, 1519, 1791
BstSLI	GKGCMC	1 cut(s)	1431
BstUI	CGCG	1 cut(s)	1460
BstV1I	GCAGC	3 cut(s)	142, 145, 910
BstV2I	GAAGAC	1 cut(s)	629
BstX2I	RGATCY	3 cut(s)	214, 784, 1228
BstYI	RGATCY	3 cut(s)	214, 784, 1228
Bsu15I	ATCGAT	2 cut(s)	703, 1510
BsuRI	GGCC	2 cut(s)	146, 1020
BsuTUI	ATCGAT	2 cut(s)	703, 1510
BtgI	CCRYGG	1 cut(s)	162
BtgZI	GCGATG	1 cut(s)	360
BtrI	CACGTC	1 cut(s)	1446
BtsCI	GGATG	5 cut(s)	508, 1173, 1360, 1474, 1816
BtsIMutI	CAGTG	4 cut(s)	376, 1142, 1333, 1664
BveI	ACCTGC	1 cut(s)	1286
Cac8I	GCNNGC	6 cut(s)	7, 153, 169, 173, 337, 1360
CciI	TCATGA	1 cut(s)	393
CfoI	GCGC	2 cut(s)	126, 978
Cfr10I	RCCGGY	1 cut(s)	1737
Cfr13I	GGNCC	5 cut(s)	449, 466, 665, 709, 1633
ClaI	ATCGAT	2 cut(s)	703, 1510
CsiI	ACCWGGT	1 cut(s)	661
Csp6I	GTAC	1 cut(s)	1601
CviAII	CATG	6 cut(s)	394, 440, 961, 973, 1270, 1559
CviQI	GTAC	1 cut(s)	1601
DdeI	CTNAG	6 cut(s)	519, 582, 877, 1086, 1149, 1479
DinI	GGCGCC	1 cut(s)	125
DraI	TTTAAA	1 cut(s)	747
DraIII	CACNNNGTG	1 cut(s)	312
Eam1104I	CTCTTC	2 cut(s)	1434, 1793
EarI	CTCTTC	2 cut(s)	1434, 1793
Ecl136II	GAGCTC	1 cut(s)	1688
Eco130I	CCWWGG	1 cut(s)	650
Eco24I	GRGCYC	2 cut(s)	1221, 1690
Eco31I	GGTCTC	1 cut(s)	1304
Eco32I	GATATC	2 cut(s)	367, 1375
Eco47I	GGWCC	5 cut(s)	449, 466, 665, 709, 1633
Eco53kI	GAGCTC	1 cut(s)	1688
Eco57I	CTGAAG	3 cut(s)	219, 263, 753
Eco91I	GGTNACC	1 cut(s)	1701
EcoICRI	GAGCTC	1 cut(s)	1688
EcoO109I	RGGNCCY	2 cut(s)	449, 709
EcoO65I	GGTNACC	1 cut(s)	1701
EcoRI	GAATTC	1 cut(s)	1103
EcoRII	CCWGG	8 cut(s)	571, 661, 712, 722, 753, 858, 1020, 1519
EcoRV	GATATC	2 cut(s)	367, 1375
EcoT14I	CCWWGG	1 cut(s)	650
EcoT22I	ATGCAT	1 cut(s)	1576
EcoT38I	GRGCYC	2 cut(s)	1221, 1690
EgeI	GGCGCC	1 cut(s)	125
EheI	GGCGCC	1 cut(s)	125
ErhI	CCWWGG	1 cut(s)	650
Esp3I	CGTCTC	1 cut(s)	1436
FaeI	CATG	6 cut(s)	397, 443, 964, 976, 1273, 1562
FaqI	GGGAC	2 cut(s)	695, 705
FatI	CATG	6 cut(s)	393, 439, 960, 972, 1269, 1558
FauI	CCCGC	1 cut(s)	1465
FauNDI	CATATG	1 cut(s)	642
FbaI	TGATCA	1 cut(s)	1621
Fnu4HI	GCNGC	4 cut(s)	156, 159, 921, 924
FokI	GGATG	5 cut(s)	515, 1180, 1367, 1461, 1823
FriOI	GRGCYC	2 cut(s)	1221, 1690
Fsp4HI	GCNGC	4 cut(s)	156, 159, 921, 924
FspAI	RTGCGCAY	1 cut(s)	977
FspBI	CTAG	3 cut(s)	411, 531, 1007
FspI	TGCGCA	1 cut(s)	977
GlaI	GCGC	2 cut(s)	125, 977
GluI	GCNGC	4 cut(s)	156, 159, 921, 924
GsaI	CCCAGC	1 cut(s)	387
GsuI	CTGGAG	1 cut(s)	1309
HaeII	RGCGCY	1 cut(s)	127
HaeIII	GGCC	2 cut(s)	146, 1020
HapII	CCGG	4 cut(s)	127, 1313, 1738, 1793
HhaI	GCGC	2 cut(s)	126, 978
Hin1I	GRCGYC	1 cut(s)	124
Hin1II	CATG	6 cut(s)	397, 443, 964, 976, 1273, 1562
Hin6I	GCGC	2 cut(s)	124, 976
HinP1I	GCGC	2 cut(s)	124, 976
HincII	GTYRAC	4 cut(s)	773, 1552, 1735, 1755
HindII	GTYRAC	4 cut(s)	773, 1552, 1735, 1755
HindIII	AAGCTT	2 cut(s)	965, 1418
HinfI	GANTC	8 cut(s)	53, 390, 506, 727, 1075, 1318, 1489, 1760
HpaI	GTTAAC	1 cut(s)	1755
HpaII	CCGG	4 cut(s)	127, 1313, 1738, 1793
HphI	GGTGA	4 cut(s)	298, 470, 557, 1658
Hpy166II	GTNNAC	5 cut(s)	773, 1092, 1552, 1735, 1755
Hpy188I	TCNGA	4 cut(s)	58, 496, 511, 676
Hpy8I	GTNNAC	5 cut(s)	773, 1092, 1552, 1735, 1755
HpyAV	CCTTC	7 cut(s)	53, 82, 136, 256, 457, 846, 1827
HpyCH4III	ACNGT	1 cut(s)	310
HpyCH4IV	ACGT	3 cut(s)	775, 1445, 1534
HpyCH4V	TGCA	9 cut(s)	80, 335, 983, 1121, 1295, 1331, 1362, 1574, 1683
HpyF10VI	GCNNNNNNNGC	5 cut(s)	152, 164, 917, 973, 1426
HpyF3I	CTNAG	6 cut(s)	519, 582, 877, 1086, 1149, 1479
HpySE526I	ACGT	3 cut(s)	775, 1445, 1534
Hsp92I	GRCGYC	1 cut(s)	124
Hsp92II	CATG	6 cut(s)	397, 443, 964, 976, 1273, 1562
HspAI	GCGC	2 cut(s)	124, 976
KasI	GGCGCC	1 cut(s)	123
KflI	GGGWCCC	1 cut(s)	709
Kpn2I	TCCGGA	1 cut(s)	1312
Ksp22I	TGATCA	1 cut(s)	1621
KspAI	GTTAAC	1 cut(s)	1755
LmnI	GCTCC	3 cut(s)	62, 352, 917
Lsp1109I	GCAGC	3 cut(s)	142, 145, 910
LweI	GCATC	4 cut(s)	364, 636, 1345, 1516
MabI	ACCWGGT	1 cut(s)	661
MaeI	CTAG	3 cut(s)	411, 531, 1007
MaeII	ACGT	3 cut(s)	775, 1445, 1534
MaeIII	GTNAC	2 cut(s)	937, 1701
MbiI	CCGCTC	1 cut(s)	920
MboII	GAAGA	9 cut(s)	97, 109, 125, 128, 212, 629, 703, 1451, 1780
MfeI	CAATTG	1 cut(s)	1233
MflI	RGATCY	3 cut(s)	214, 784, 1228
MhlI	GDGCHC	3 cut(s)	1221, 1431, 1690
MluCI	AATT	9 cut(s)	290, 512, 1049, 1103, 1233, 1280, 1367, 1541, 1583
Mly113I	GGCGCC	1 cut(s)	124
MlyI	GAGTC	2 cut(s)	1069, 1498
MmeI	TCCRAC	3 cut(s)	242, 1392, 1733
Mph1103I	ATGCAT	1 cut(s)	1576
MroI	TCCGGA	1 cut(s)	1312
MroXI	GAANNNNTTC	1 cut(s)	968
MslI	CAYNNNNRTG	2 cut(s)	531, 1212
MspCI	CTTAAG	2 cut(s)	340, 1349
MspI	CCGG	4 cut(s)	127, 1313, 1738, 1793
MspR9I	CCNGG	9 cut(s)	573, 663, 714, 724, 755, 860, 1022, 1521, 1793
MunI	CAATTG	1 cut(s)	1233
MvaI	CCWGG	8 cut(s)	573, 663, 714, 724, 755, 860, 1022, 1521
MvnI	CGCG	1 cut(s)	1460
MwoI	GCNNNNNNNGC	5 cut(s)	152, 164, 917, 973, 1426
NarI	GGCGCC	1 cut(s)	124
NciI	CCSGG	1 cut(s)	1793
NdeI	CATATG	1 cut(s)	642
NlaIII	CATG	6 cut(s)	397, 443, 964, 976, 1273, 1562
NlaIV	GGNNCC	5 cut(s)	125, 216, 710, 711, 857
NmuCI	GTSAC	1 cut(s)	937
NsbI	TGCGCA	1 cut(s)	977
NsiI	ATGCAT	1 cut(s)	1576
NspV	TTCGAA	1 cut(s)	1211
PagI	TCATGA	1 cut(s)	393
PdmI	GAANNNNTTC	1 cut(s)	968
PfeI	GAWTC	6 cut(s)	53, 390, 506, 727, 1318, 1760
PflMI	CCANNNNNTGG	2 cut(s)	475, 1715
PfoI	TCCNGGA	2 cut(s)	571, 858
PkrI	GCNGC	4 cut(s)	157, 160, 922, 925
PleI	GAGTC	2 cut(s)	1069, 1497
PluTI	GGCGCC	1 cut(s)	127
PpsI	GAGTC	2 cut(s)	1069, 1497
PpuMI	RGGWCCY	2 cut(s)	449, 709
Psp124BI	GAGCTC	1 cut(s)	1690
Psp1406I	AACGTT	2 cut(s)	775, 1534
Psp5II	RGGWCCY	2 cut(s)	449, 709
Psp6I	CCWGG	8 cut(s)	571, 661, 712, 722, 753, 858, 1020, 1519
PspEI	GGTNACC	1 cut(s)	1701
PspFI	CCCAGC	1 cut(s)	383
PspGI	CCWGG	8 cut(s)	571, 661, 712, 722, 753, 858, 1020, 1519
PspN4I	GGNNCC	5 cut(s)	125, 216, 710, 711, 857
PspPI	GGNCC	5 cut(s)	449, 466, 665, 709, 1633
PspPPI	RGGWCCY	2 cut(s)	449, 709
PsuI	RGATCY	3 cut(s)	214, 784, 1228
RsaI	GTAC	1 cut(s)	1602
RsaNI	GTAC	1 cut(s)	1601
RseI	CAYNNNNRTG	2 cut(s)	531, 1212
SacI	GAGCTC	1 cut(s)	1690
SatI	GCNGC	4 cut(s)	156, 159, 921, 924
Sau96I	GGNCC	5 cut(s)	449, 466, 665, 709, 1633
SchI	GAGTC	2 cut(s)	1069, 1498
ScrFI	CCNGG	9 cut(s)	573, 663, 714, 724, 755, 860, 1022, 1521, 1793
SduI	GDGCHC	3 cut(s)	1221, 1431, 1690
SexAI	ACCWGGT	1 cut(s)	661
SfaNI	GCATC	4 cut(s)	364, 636, 1345, 1516
SfoI	GGCGCC	1 cut(s)	125
SfuI	TTCGAA	1 cut(s)	1211
SinI	GGWCC	5 cut(s)	449, 466, 665, 709, 1633
SmiMI	CAYNNNNRTG	2 cut(s)	531, 1212
SmlI	CTYRAG	5 cut(s)	340, 359, 1349, 1628, 1691
SmoI	CTYRAG	5 cut(s)	340, 359, 1349, 1628, 1691
Sse9I	AATT	9 cut(s)	290, 512, 1049, 1103, 1233, 1280, 1367, 1541, 1583
SsiI	CCGC	3 cut(s)	32, 920, 1458
SspDI	GGCGCC	1 cut(s)	123
SspI	AATATT	3 cut(s)	792, 816, 1771
SspMI	CTAG	3 cut(s)	411, 531, 1007
SstI	GAGCTC	1 cut(s)	1690
StyD4I	CCNGG	9 cut(s)	571, 661, 712, 722, 753, 858, 1020, 1519, 1791
StyI	CCWWGG	1 cut(s)	650
TaaI	ACNGT	1 cut(s)	310
TaiI	ACGT	3 cut(s)	778, 1448, 1537
TaqI	TCGA	7 cut(s)	703, 808, 885, 1211, 1221, 1266, 1510
TaqII	GACCGA	1 cut(s)	1650
TasI	AATT	9 cut(s)	290, 512, 1049, 1103, 1233, 1280, 1367, 1541, 1583
TauI	GCSGC	1 cut(s)	923
TfiI	GAWTC	6 cut(s)	53, 390, 506, 727, 1318, 1760
TscAI	CASTG	4 cut(s)	376, 1149, 1333, 1671
TseFI	GTSAC	1 cut(s)	937
TseI	GCWGC	3 cut(s)	155, 158, 923
Tsp45I	GTSAC	1 cut(s)	937
TspGWI	ACGGA	1 cut(s)	97
TspRI	CASTG	4 cut(s)	376, 1149, 1333, 1671
Van91I	CCANNNNNTGG	2 cut(s)	475, 1715
Vha464I	CTTAAG	2 cut(s)	340, 1349
VpaK11BI	GGWCC	5 cut(s)	449, 466, 665, 709, 1633
XapI	RAATTY	4 cut(s)	290, 512, 1103, 1367
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1716
XmnI	GAANNNNTTC	1 cut(s)	968
XspI	CTAG	3 cut(s)	411, 531, 1007
Zsp2I	ATGCAT	1 cut(s)	1576

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.