Rorug04G0028100

Lysyl-tRNA synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005056
Physical Location & Seq
Forward (+)
3935200 .. 3935793
594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0028100.1

Sequence Viewer

Length: 594 bp
ATGCAACTGGGTAGTGTTACTTTGTTTTTCTTATTCATCTTCAGTTTGCTTCCATCACAGTATGGCGCTGAAGTACATAACATAACTTCTTCACACCCATTATCTGATGGAGAAACTCTAGTCTCTCCTGGCTTAATATACGAATTGGGCTTCTTCAGTCCTAAGAGTTCTGCTAACAAGTATTTGGGGTTGTGGCACAAAAGTATATTTCCCCGCAAGTATGTATGGGTGGCTAACAGAGATAATCCTCTTGCAGATACAGACACCTTGGCTAGTTTGAGAATTGGAAGCAGCGGGAGTCTGGAGCTCGTAGATGGAAAACAGAGTTCTTTCTGGTCAACCAATATATCTAATTGTTCATCTGCAGTTCTCTTAGATAATGGAAACTTCGTTGTCAAAGATGTTATGGGAGCTGTTATGTGGGAGAGCTTTAATAATCCTAGTGACTCACTTCTGCCAAGCATGTTGATGGGATATGATAGTGGTTCTGGAAAACAGAATTTGTTGACATCTTGGAAAAGTGAAAATGATCCATCACCGGGGATATTCTCGGCCGGACTGTCAGCAGAGTTGCCAGCACAAGCGTTCATTTAG

Protein Analysis

197

Amino Acids

21.34

Weight (kDa)

4.88

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 173 5.7e-27 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 214, 294
AclWI GGATC 1 cut(s) 524
AcoI YGGCCR 1 cut(s) 552
AcsI RAATTY 1 cut(s) 499
AcuI CTGAAG 3 cut(s) 25, 90, 139
AfaI GTAC 1 cut(s) 75
AfiI CCNNNNNNNGG 1 cut(s) 539
AjnI CCWGG 1 cut(s) 127
AluBI AGCT 3 cut(s) 307, 413, 429
AluI AGCT 3 cut(s) 307, 413, 429
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 1 cut(s) 127
AlwI GGATC 1 cut(s) 524
AoxI GGCC 1 cut(s) 552
ApeKI GCWGC 1 cut(s) 291
ApoI RAATTY 1 cut(s) 499
AspLEI GCGC 1 cut(s) 68
AsuC2I CCSGG 1 cut(s) 540
AsuHPI GGTGA 1 cut(s) 528
BanII GRGCYC 1 cut(s) 309
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 1 cut(s) 303
BccI CCATC 5 cut(s) 61, 101, 308, 463, 541
BciT130I CCWGG 1 cut(s) 129
BcnI CCSGG 1 cut(s) 540
BcoDI GTCTC 1 cut(s) 127
BfaI CTAG 3 cut(s) 119, 273, 441
BfmI CTRYAG 1 cut(s) 363
BfoI RGCGCY 1 cut(s) 69
BisI GCNGC 1 cut(s) 292
BlsI GCNGC 1 cut(s) 293
Bme1390I CCNGG 2 cut(s) 129, 540
BmrFI CCNGG 2 cut(s) 129, 540
BmrI ACTGGG 1 cut(s) 17
BmuI ACTGGG 1 cut(s) 17
BpmI CTGGAG 1 cut(s) 323
BpuMI CCSGG 1 cut(s) 540
BsaJI CCNNGG 2 cut(s) 267, 539
Bsc4I CCNNNNNNNGG 1 cut(s) 539
Bse1I ACTGG 1 cut(s) 12
BseBI CCWGG 1 cut(s) 129
BseDI CCNNGG 2 cut(s) 267, 539
BseLI CCNNNNNNNGG 1 cut(s) 539
BseNI ACTGG 1 cut(s) 12
BseX3I CGGCCG 1 cut(s) 552
BseXI GCAGC 1 cut(s) 303
Bsh1285I CGRYCG 1 cut(s) 555
BshFI GGCC 1 cut(s) 554
BsiEI CGRYCG 1 cut(s) 555
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 2 cut(s) 539, 555
BslI CCNNNNNNNGG 1 cut(s) 539
BsmAI GTCTC 1 cut(s) 127
BsnI GGCC 1 cut(s) 554
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 1 cut(s) 529
BspACI CCGC 2 cut(s) 214, 294
BspANI GGCC 1 cut(s) 554
BspMAI CTGCAG 1 cut(s) 367
BspPI GGATC 1 cut(s) 524
BsrI ACTGG 1 cut(s) 12
BssECI CCNNGG 2 cut(s) 267, 539
BssMI GATC 1 cut(s) 529
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 2 cut(s) 60, 561
BstC8I GCNNGC 1 cut(s) 576
BstDEI CTNAG 2 cut(s) 162, 373
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 1 cut(s) 532
BstMAI GTCTC 1 cut(s) 127
BstMBI GATC 1 cut(s) 529
BstMCI CGRYCG 1 cut(s) 555
BstNI CCWGG 1 cut(s) 129
BstNSI RCATGY 1 cut(s) 466
BstSCI CCNGG 2 cut(s) 127, 538
BstSFI CTRYAG 1 cut(s) 363
BstV1I GCAGC 1 cut(s) 303
BstZI CGGCCG 1 cut(s) 552
BsuRI GGCC 1 cut(s) 554
Cac8I GCNNGC 1 cut(s) 576
CfoI GCGC 1 cut(s) 68
Csp6I GTAC 1 cut(s) 74
CviAII CATG 1 cut(s) 463
CviJI RGCY 8 cut(s) 132, 150, 233, 272, 307, 413, 429, 554
CviKI_1 RGCY 8 cut(s) 132, 150, 233, 272, 307, 413, 429, 554
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 2 cut(s) 162, 373
DpnI GATC 1 cut(s) 531
DpnII GATC 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 552
EagI CGGCCG 1 cut(s) 552
Ecl136II GAGCTC 1 cut(s) 307
EclXI CGGCCG 1 cut(s) 552
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 309
Eco52I CGGCCG 1 cut(s) 552
Eco53kI GAGCTC 1 cut(s) 307
Eco57I CTGAAG 3 cut(s) 25, 90, 139
EcoICRI GAGCTC 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 1 cut(s) 466
FatI CATG 1 cut(s) 462
FauI CCCGC 2 cut(s) 221, 287
Fnu4HI GCNGC 1 cut(s) 292
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 1 cut(s) 292
FspBI CTAG 3 cut(s) 119, 273, 441
GlaI GCGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 292
GsuI CTGGAG 1 cut(s) 323
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 1 cut(s) 554
HapII CCGG 2 cut(s) 539, 555
HhaI GCGC 1 cut(s) 68
Hin1II CATG 1 cut(s) 466
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 2 cut(s) 339, 507
HindII GTYRAC 2 cut(s) 339, 507
HinfI GANTC 2 cut(s) 298, 446
HpaII CCGG 2 cut(s) 539, 555
HphI GGTGA 1 cut(s) 528
Hpy166II GTNNAC 2 cut(s) 339, 507
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 2 cut(s) 302, 489
Hpy8I GTNNAC 2 cut(s) 339, 507
HpyCH4III ACNGT 2 cut(s) 60, 561
HpyCH4V TGCA 3 cut(s) 4, 254, 365
HpyF3I CTNAG 2 cut(s) 162, 373
Hsp92II CATG 1 cut(s) 466
HspAI GCGC 1 cut(s) 66
Kzo9I GATC 1 cut(s) 529
LmnI GCTCC 2 cut(s) 304, 410
LpnPI CCDG 8 cut(s) 114, 141, 287, 319, 474, 552, 568, 588
Lsp1109I GCAGC 1 cut(s) 303
MaeI CTAG 3 cut(s) 119, 273, 441
MaeIII GTNAC 2 cut(s) 16, 443
MalI GATC 1 cut(s) 531
MboI GATC 1 cut(s) 529
MboII GAAGA 3 cut(s) 31, 81, 145
MhlI GDGCHC 1 cut(s) 309
MluCI AATT 4 cut(s) 143, 282, 352, 499
MlyI GAGTC 2 cut(s) 307, 440
MnlI CCTC 1 cut(s) 258
MseI TTAA 2 cut(s) 134, 432
MslI CAYNNNNRTG 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 294
MspI CCGG 2 cut(s) 539, 555
MspR9I CCNGG 2 cut(s) 129, 540
MvaI CCWGG 1 cut(s) 129
NciI CCSGG 1 cut(s) 540
NdeII GATC 1 cut(s) 529
NlaIII CATG 1 cut(s) 466
NmeAIII GCCGAG 1 cut(s) 530
NmuCI GTSAC 1 cut(s) 443
NspI RCATGY 1 cut(s) 466
PkrI GCNGC 1 cut(s) 293
PleI GAGTC 2 cut(s) 306, 440
PpsI GAGTC 2 cut(s) 306, 440
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
PstI CTGCAG 1 cut(s) 367
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 1 cut(s) 309
SaqAI TTAA 2 cut(s) 134, 432
SatI GCNGC 1 cut(s) 292
Sau3AI GATC 1 cut(s) 529
SchI GAGTC 2 cut(s) 307, 440
ScrFI CCNGG 2 cut(s) 129, 540
SduI GDGCHC 1 cut(s) 309
SetI ASST 4 cut(s) 269, 309, 415, 431
SfcI CTRYAG 1 cut(s) 363
SmiMI CAYNNNNRTG 1 cut(s) 467
Sse9I AATT 4 cut(s) 143, 282, 352, 499
SsiI CCGC 2 cut(s) 214, 294
SspMI CTAG 3 cut(s) 119, 273, 441
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 2 cut(s) 127, 538
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 2 cut(s) 60, 561
TasI AATT 4 cut(s) 143, 282, 352, 499
TatI WGTACW 1 cut(s) 73
Tru1I TTAA 2 cut(s) 134, 432
Tru9I TTAA 2 cut(s) 134, 432
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 1 cut(s) 291
Tsp45I GTSAC 1 cut(s) 443
TspDTI ATGAA 3 cut(s) 25, 348, 577
XapI RAATTY 1 cut(s) 499
XceI RCATGY 1 cut(s) 466
XspI CTAG 3 cut(s) 119, 273, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.