AT3G45630

RING/Ubox like zinc-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001311
Physical Location & Seq
Reverse (-)
16748092 .. 16753843
5752 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G45630.1

Sequence Viewer

Length: 2970 bp
ATGAGTGATTACGGTGAAAAGACTTGCCCTTTGTGTGCTGAGGAGATGGATTTGACTGACCAGCAATTGAAGCCTTGCAAATGTGGCTATCAGATTTGTGTTTGGTGCTGGCACCATATAATGGACATGGCAGAGAAAGATCAATCAGAAGGGCGCTGTCCAGCTTGCCGCACTCCATATGACAAAGAAAAGATTGTTGGGATGACTGTTGATCAGGAGAGACTGGCCTCTGAAGGCAACATGGATCGCAAAAAAATACAAAAGTCGAAACCGAAATCTTCTGATGGGAGAAAGCCACTAACAAGTGTCCGAGTGGTTCAAAGAAATCTTGTTTACATTGTTGGATTGCCCCTTAATCTAGCAGATGAAGATCTTCTCCAGCGTAAAGAATATTTTGGTCAGTATGGAAAGGTTCTAAAGGTTTCGATGTCCCGAACAGCAACAGGTCTCATCCAACAGTTTCCTAACAACACATGCAGTGTATACATCACATATGGCAAAGAGGAAGAGGCCATTCGATGTATTCAGTCTGTTCATGGGTTTATCTTGGATGGTAAAGCACTGAAGGCATGTTTTGGGACAACCAAGTATTGCCATGCATGGTTGAGAAACGTGGCATGCAACAATCAGGATTGCCTCTATTTGCATGAGGTTGGCTCTCAAGAGGATAGTTTTACAAAAGATGAGATCATATCAGCCCATACAAGAGTTCAGCAAATCACTGGAGCAACAAATACTATGCAATACCGTTCAGGGAGCATGCTACCTCCACCATTGGATGCTTATACCAGTGACAGTTCTACTGGAAATCCAATTGCAAAAGTTCCTTCTAGTACTTCAGTTAGTGCTCCTAAGAGTTCCCCACCTAGTGGGAGCTCGGGTAAATCAACTGCTCTTCCTGCTGCAGCATCATGGGGGGCACGTTTAACAAATCAACACTCTTTAGCAACTTCAGCTTTGTCAAATGGATCTTTGGATAATCAAAGATCAACATCAGAGAATGGAACTTTGGCTACGTCTACAGTTGTTACAAAGGCAGCTAATGGGCCTGTATCCTCTAGTAACAGTCTTCAGAAAGCACCTCTCAAGGAAGAAATTCAATCATTGGCTGAAAAAAGCAAACCTGGTGTGTTGAAGCCTTTGCAGCAGAAGATTGTGTTGGATCCCGAGTCCAAGAGAACTACATCACCTAATAGAGACCCTAGTAGCAATCAGATATCCTGTTTAGTAGAGTCTTCCTACAATAGCAGAGTCATTGACAAGCCTTCTGCTGTTGAGAATTCTCTTGAACACACAAGTGAAATTGCTGAAGATGTCTTCGATGTTGGTAAATTGTCTGCTGATGTTGCATGGATGGGGATAACTACAAATTCTAGGGATGAAACCCCTGGTGTTCCAGTGGTTATCGGTACTCATTGTGATCTTGGTTCTATTACACAATCTGATAACGATGTACAAAATCTCGAGCAATGTAGAAAACAGTCTCCTACAAACACTTATGCAGAAGCTGACATATCACTGAATGGAATTCATGGTTCAAGGCCTGAGTGGGATTGGAGATCAGGTTTGCAATCCCAGATAGATGTTAAGGAACCGTTGGAAGTCAATGATTTCTCATCATTCAATAACAACAGACGTGGTATTGCAGAGGCTGTTAGCCACTCGACTTCTAAGTTTAGTTCATCAATCTCAATTCTGGATTCAAATCATCTAGCTTCTCGTTCGTTCCAGAACCGGGAAACTTCTTGTGGGATGGATTCAAAAACTGGATCCTCCTTTGAAATAGGTAGTGATAGATTGCATCTTCCAAATGGATTTTCTGAGAAAGCTATGTCCAATATGGAGCATTCATTATTTGCTAATGAAGGCAGAAGCAACATTCAGAATACAGAGGATGATATCATTTCAAACATTTTAGACTTTGATCCCTGGGATGAATCGTTGACTTCGCAACACAATTTTGCAAAATTACTTGGTCAGAGTGATCATAGGGCCAGTACTCTGGAATCTTCTAACCTCCTAAAGCAACATAATGACCAGTCTAGGTTCTCTTTTGCTCGGCATGAAGAATCAAATAGTCAAGCGTATGACAATAGAAGTTACAGCATATATGGGCAGTTGTCAAGAGATCAGCCTCTTCAAGAGTTTGGAGCGAACCGTGATATGTATCAGGATAAGCTTGGCAGTCAAAATGGTTTTGCCTCAAACTATTCCGGTGGATATGAACAATTTGCTACTAGTCCTGGATTATCTTCTTACAAGAGTCCTGTGGCGAGGACTCAAGTTTCTGCTCCTCCCGGATTTTCTGCTCCCAACAGGTTACCTCCTCCCGGTTTCTCTTCACATCAAAGAGGCGACCTGTCTTCTGATATTGCATCAGGAACTCGTTTACTTGACTCAGCCAATTTGTTGAGGAACGCATATCATGTACCACCTCCAAGTGGAAATTTGAATGCTGCAGGAGATATTGAGTTTATTGATCCTGCAATTTTAGCTGTTGGAAGGGGAAGACTTCATAATGGGATGGAAACAGCAGACTTTGATTTGAGATCAGGGTTCTCTTCTCAACTGAATTCATTTGATAATGATGCAAGACTCCAATTGTTGGCGCAGAGGTCTCTGGCTGCACAACAAGTCAATGGATTTCACGATCCCAGGAACGTAAACAACTTCTCTTCTTCGTTCTCTGACCCATATGGGATTAGCTCGAGGCCAACGGATCAAACACAAGGAACTGGCTTGTCTCCTTTCACACAGCTGCCTAGACAAGCTTCAGCAAATCCATTGTTGTCAAATGGACACTGGGATAATAAGTGGAATGAACCACAAAGTGGTAACAATCTTGGGATTACCCAGCTTCTAAGGAATGAACGGATGGGTTTTAATGACAACGTTTACAGCGGTTTTGAAGAGCCTAAATTCCGGAGGCCCGGTCCTGGAGATCCGTATAACAGAACATACGGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000956 GO:0002682 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010468 GO:0010564 GO:0010605 GO:0010629 GO:0010948 GO:0016070 GO:0016071 GO:0016567 GO:0016740 GO:0019222 GO:0019439 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0023052 GO:0030163 GO:0030330 GO:0031570 GO:0031571 GO:0032446 GO:0033554 GO:0034641 GO:0034655 GO:0035556 GO:0036211 GO:0042770 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045595 GO:0045637 GO:0045652 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051726 GO:0051865 GO:0060255 GO:0065007 GO:0070647 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0090068 GO:0090304 GO:0140096 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1903706 GO:2000026 GO:2000045 GO:2000134
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
RRM_1 zf-RING_4

Protein Analysis

989

Amino Acids

108.44

Weight (kDa)

5.85

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_4 PF14570 9 - 61 6.5e-20 RING/Ubox like zinc-binding domain
RRM_1 PF00076 111 - 189 8.3e-06 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 111
AccB7I CCANNNNNTGG 4 cut(s) 121, 869, 2605, 2831
AccI GTMKAC 2 cut(s) 483, 1019
AccIII TCCGGA 1 cut(s) 2922
AciI CCGC 2 cut(s) 169, 2901
AclI AACGTT 1 cut(s) 2892
AcsI RAATTY 7 cut(s) 1095, 1279, 1369, 1527, 2446, 2572, 2918
AcuI CTGAAG 7 cut(s) 252, 584, 822, 936, 1055, 1329, 2757
AdeI CACNNNGTG 2 cut(s) 869, 2831
AfaI GTAC 5 cut(s) 835, 1411, 1455, 1999, 2430
AfiI CCNNNNNNNGG 8 cut(s) 121, 869, 1735, 2330, 2441, 2605, 2831, 2936
AhlI ACTAGT 1 cut(s) 2237
AjiI CACGTC 1 cut(s) 1637
AjnI CCWGG 6 cut(s) 1123, 1387, 1928, 2242, 2654, 2935
AjuI GAANNNNNNNTTGG 8 cut(s) 180, 212, 992, 1024, 1142, 1174, 1801, 1833
AleI CACNNNNGTG 1 cut(s) 1296
Alw21I GWGCWC 2 cut(s) 850, 878
Alw26I GTCTC 6 cut(s) 214, 452, 1191, 1488, 2622, 2748
AlwNI CAGNNNCTG 2 cut(s) 1508, 1652
Ama87I CYCGRG 4 cut(s) 877, 1166, 1463, 2707
Aor13HI TCCGGA 1 cut(s) 2922
AoxI GGCC 7 cut(s) 225, 510, 1046, 1541, 1992, 2711, 2927
ApeKI GCWGC 7 cut(s) 902, 905, 1037, 1144, 2456, 2624, 2758
ApoI RAATTY 7 cut(s) 1095, 1279, 1369, 1527, 2446, 2572, 2918
ArsI GACNNNNNNTTYG 2 cut(s) 13, 45
Asp700I GAANNNNTTC 2 cut(s) 372, 1095
AspLEI GCGC 2 cut(s) 156, 2611
AspS9I GGNCC 4 cut(s) 1046, 1992, 2928, 2933
AsuC2I CCSGG 4 cut(s) 1736, 2298, 2331, 2931
AsuHPI GGTGA 2 cut(s) 26, 1179
AvaI CYCGRG 4 cut(s) 877, 1166, 1463, 2707
AvaII GGWCC 1 cut(s) 2933
BaeGI GKGCMC 1 cut(s) 922
BamHI GGATCC 2 cut(s) 1162, 1769
BanI GGYRCC 1 cut(s) 111
BanII GRGCYC 1 cut(s) 878
BbsI GAAGAC 5 cut(s) 1061, 1227, 1309, 2355, 2515
Bbv12I GWGCWC 2 cut(s) 850, 878
BbvCI CCTCAGC 1 cut(s) 39
BbvI GCAGC 7 cut(s) 889, 917, 1049, 1156, 2443, 2611, 2745
BccI CCATC 7 cut(s) 40, 278, 545, 1348, 1747, 2518, 2869
BciT130I CCWGG 6 cut(s) 1125, 1389, 1930, 2244, 2656, 2937
BciVI GTATCC 1 cut(s) 1063
BclI TGATCA 2 cut(s) 211, 1984
BcnI CCSGG 4 cut(s) 1736, 2298, 2331, 2931
BcoDI GTCTC 6 cut(s) 214, 452, 1191, 1488, 2622, 2748
BcuI ACTAGT 1 cut(s) 2237
BfmI CTRYAG 3 cut(s) 903, 1020, 2457
BfoI RGCGCY 1 cut(s) 157
BfuI GTATCC 1 cut(s) 1063
BglII AGATCT 1 cut(s) 370
BisI GCNGC 8 cut(s) 169, 903, 906, 1038, 1145, 2457, 2625, 2759
BlsI GCNGC 8 cut(s) 170, 904, 907, 1039, 1146, 2458, 2626, 2760
BmcAI AGTACT 2 cut(s) 835, 1999
Bme18I GGWCC 1 cut(s) 2933
BmeT110I CYCGRG 4 cut(s) 877, 1166, 1463, 2707
BmgBI CACGTC 1 cut(s) 1637
BmgT120I GGNCC 4 cut(s) 1046, 1992, 2928, 2933
BmiI GGNNCC 4 cut(s) 113, 1164, 1593, 1771
BmrI ACTGGG 1 cut(s) 2812
BmsI GCATC 5 cut(s) 769, 917, 1810, 2384, 2578
BmuI ACTGGG 1 cut(s) 2812
BpiI GAAGAC 5 cut(s) 1061, 1227, 1309, 2355, 2515
BplI GAGNNNNNCTC 2 cut(s) 641, 673
BpmI CTGGAG 3 cut(s) 362, 744, 2958
Bpu10I CCTNAGC 1 cut(s) 39
BpuEI CTTGAG 3 cut(s) 645, 1070, 2265
BpuMI CCSGG 4 cut(s) 1736, 2298, 2331, 2931
BsaBI GATNNNNATC 4 cut(s) 369, 991, 1704, 2166
BsaI GGTCTC 3 cut(s) 452, 1191, 2622
BsaJI CCNNGG 4 cut(s) 1387, 1928, 1929, 2654
BsaWI WCCGGW 2 cut(s) 2213, 2922
BsaXI ACNNNNNCTCC 6 cut(s) 865, 895, 1549, 1579, 2917, 2947
Bsc4I CCNNNNNNNGG 8 cut(s) 121, 869, 1735, 2330, 2441, 2605, 2831, 2936
Bse3DI GCAATG 1 cut(s) 1475
Bse8I GATNNNNATC 4 cut(s) 369, 991, 1704, 2166
BseAI TCCGGA 1 cut(s) 2922
BseBI CCWGG 6 cut(s) 1125, 1389, 1930, 2244, 2656, 2937
BseDI CCNNGG 4 cut(s) 1387, 1928, 1929, 2654
BseJI GATNNNNATC 4 cut(s) 369, 991, 1704, 2166
BseLI CCNNNNNNNGG 8 cut(s) 121, 869, 1735, 2330, 2441, 2605, 2831, 2936
BseMI GCAATG 1 cut(s) 1475
BseMII CTCAG 4 cut(s) 30, 1536, 1812, 2412
BseRI GAGGAG 3 cut(s) 56, 2283, 2316
BseSI GKGCMC 1 cut(s) 922
BseXI GCAGC 7 cut(s) 889, 917, 1049, 1156, 2443, 2611, 2745
BseYI CCCAGC 1 cut(s) 2853
BsgI GTGCAG 1 cut(s) 2610
BshFI GGCC 7 cut(s) 227, 512, 1048, 1543, 1994, 2713, 2929
BshNI GGYRCC 1 cut(s) 111
BsiHKAI GWGCWC 2 cut(s) 850, 878
BsiHKCI CYCGRG 4 cut(s) 877, 1166, 1463, 2707
BsiSI CCGG 6 cut(s) 1735, 2214, 2298, 2331, 2923, 2931
BslFI GGGAC 2 cut(s) 415, 592
BslI CCNNNNNNNGG 8 cut(s) 121, 869, 1735, 2330, 2441, 2605, 2831, 2936
BsmAI GTCTC 6 cut(s) 214, 452, 1191, 1488, 2622, 2748
BsmFI GGGAC 2 cut(s) 415, 592
BsmI GAATGC 2 cut(s) 1846, 2458
BsnI GGCC 7 cut(s) 227, 512, 1048, 1543, 1994, 2713, 2929
Bso31I GGTCTC 3 cut(s) 452, 1191, 2622
BsoBI CYCGRG 4 cut(s) 877, 1166, 1463, 2707
Bsp1286I GDGCHC 3 cut(s) 850, 878, 922
Bsp13I TCCGGA 1 cut(s) 2922
Bsp1407I TGTACA 1 cut(s) 1453
BspACI CCGC 2 cut(s) 169, 2901
BspANI GGCC 7 cut(s) 227, 512, 1048, 1543, 1994, 2713, 2929
BspCNI CTCAG 4 cut(s) 31, 1537, 1813, 2411
BspEI TCCGGA 1 cut(s) 2922
BspLI GGNNCC 4 cut(s) 113, 1164, 1593, 1771
BspMAI CTGCAG 2 cut(s) 907, 2461
BspQI GCTCTTC 2 cut(s) 900, 2904
BspT107I GGYRCC 1 cut(s) 111
BspTNI GGTCTC 3 cut(s) 452, 1191, 2622
BsrDI GCAATG 1 cut(s) 1475
BsrGI TGTACA 1 cut(s) 1453
BssECI CCNNGG 4 cut(s) 1387, 1928, 1929, 2654
BssNAI GTATAC 1 cut(s) 484
Bst1107I GTATAC 1 cut(s) 484
Bst2UI CCWGG 6 cut(s) 1125, 1389, 1930, 2244, 2656, 2937
Bst6I CTCTTC 7 cut(s) 501, 900, 2142, 2344, 2566, 2680, 2904
BstAUI TGTACA 1 cut(s) 1453
BstC8I GCNNGC 4 cut(s) 110, 166, 619, 761
BstDEI CTNAG 7 cut(s) 39, 852, 1545, 1671, 1821, 2398, 2861
BstEII GGTNACC 1 cut(s) 2319
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 2 cut(s) 156, 2611
BstMAI GTCTC 6 cut(s) 214, 452, 1191, 1488, 2622, 2748
BstMWI GCNNNNNNNGC 8 cut(s) 70, 84, 566, 899, 953, 1144, 1346, 2492
BstNI CCWGG 6 cut(s) 1125, 1389, 1930, 2244, 2656, 2937
BstNSI RCATGY 4 cut(s) 477, 573, 621, 763
BstPI GGTNACC 1 cut(s) 2319
BstSFI CTRYAG 3 cut(s) 903, 1020, 2457
BstSLI GKGCMC 1 cut(s) 922
BstV1I GCAGC 7 cut(s) 889, 917, 1049, 1156, 2443, 2611, 2745
BstV2I GAAGAC 5 cut(s) 1061, 1227, 1309, 2355, 2515
BstX2I RGATCY 5 cut(s) 370, 968, 1162, 1769, 2941
BstXI CCANNNNNNTGG 1 cut(s) 2002
BstYI RGATCY 5 cut(s) 370, 968, 1162, 1769, 2941
BstZ17I GTATAC 1 cut(s) 484
BsuI GTATCC 1 cut(s) 1063
BsuRI GGCC 7 cut(s) 227, 512, 1048, 1543, 1994, 2713, 2929
BtrI CACGTC 1 cut(s) 1637
BtsI GCAGTG 1 cut(s) 484
BtsIMutI CAGTG 7 cut(s) 484, 560, 720, 796, 1404, 1517, 2800
Cac8I GCNNGC 4 cut(s) 110, 166, 619, 761
CaiI CAGNNNCTG 2 cut(s) 1508, 1652
CfoI GCGC 2 cut(s) 156, 2611
Cfr13I GGNCC 4 cut(s) 1046, 1992, 2928, 2933
CsiI ACCWGGT 1 cut(s) 1123
Csp6I GTAC 5 cut(s) 834, 1410, 1454, 1998, 2429
CspCI CAANNNNNGTGG 2 cut(s) 1618, 1653
CviQI GTAC 5 cut(s) 834, 1410, 1454, 1998, 2429
DdeI CTNAG 7 cut(s) 39, 852, 1545, 1671, 1821, 2398, 2861
DraIII CACNNNGTG 2 cut(s) 869, 2831
Eam1104I CTCTTC 7 cut(s) 501, 900, 2142, 2344, 2566, 2680, 2904
EarI CTCTTC 7 cut(s) 501, 900, 2142, 2344, 2566, 2680, 2904
Ecl136II GAGCTC 1 cut(s) 876
Eco147I AGGCCT 1 cut(s) 1543
Eco24I GRGCYC 1 cut(s) 878
Eco31I GGTCTC 3 cut(s) 452, 1191, 2622
Eco32I GATATC 2 cut(s) 1218, 1900
Eco47I GGWCC 1 cut(s) 2933
Eco53kI GAGCTC 1 cut(s) 876
Eco57I CTGAAG 7 cut(s) 252, 584, 822, 936, 1055, 1329, 2757
Eco88I CYCGRG 4 cut(s) 877, 1166, 1463, 2707
Eco91I GGTNACC 1 cut(s) 2319
EcoICRI GAGCTC 1 cut(s) 876
EcoO65I GGTNACC 1 cut(s) 2319
EcoRI GAATTC 3 cut(s) 1279, 1527, 2572
EcoRII CCWGG 6 cut(s) 1123, 1387, 1928, 2242, 2654, 2935
EcoRV GATATC 2 cut(s) 1218, 1900
EcoT22I ATGCAT 1 cut(s) 601
EcoT38I GRGCYC 1 cut(s) 878
FaqI GGGAC 2 cut(s) 415, 592
FauNDI CATATG 3 cut(s) 178, 493, 2695
FbaI TGATCA 2 cut(s) 211, 1984
FblI GTMKAC 2 cut(s) 483, 1019
Fnu4HI GCNGC 8 cut(s) 169, 903, 906, 1038, 1145, 2457, 2625, 2759
FriOI GRGCYC 1 cut(s) 878
Fsp4HI GCNGC 8 cut(s) 169, 903, 906, 1038, 1145, 2457, 2625, 2759
GlaI GCGC 2 cut(s) 155, 2610
GluI GCNGC 8 cut(s) 169, 903, 906, 1038, 1145, 2457, 2625, 2759
GsaI CCCAGC 1 cut(s) 2857
GsuI CTGGAG 3 cut(s) 362, 744, 2958
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 7 cut(s) 227, 512, 1048, 1543, 1994, 2713, 2929
HapII CCGG 6 cut(s) 1735, 2214, 2298, 2331, 2923, 2931
HhaI GCGC 2 cut(s) 156, 2611
Hin6I GCGC 2 cut(s) 154, 2609
HinP1I GCGC 2 cut(s) 154, 2609
HincII GTYRAC 1 cut(s) 1944
HindII GTYRAC 1 cut(s) 1944
HindIII AAGCTT 2 cut(s) 2177, 2769
HpaII CCGG 6 cut(s) 1735, 2214, 2298, 2331, 2923, 2931
HphI GGTGA 2 cut(s) 26, 1179
Hpy166II GTNNAC 7 cut(s) 334, 484, 1020, 1944, 2390, 2665, 2896
Hpy8I GTNNAC 7 cut(s) 334, 484, 1020, 1944, 2390, 2665, 2896
HpyAV CCTTC 7 cut(s) 143, 227, 559, 837, 1275, 1859, 2496
HpyCH4IV ACGT 6 cut(s) 612, 922, 1016, 1636, 2661, 2892
HpyF10VI GCNNNNNNNGC 8 cut(s) 70, 84, 566, 899, 953, 1144, 1346, 2492
HpyF3I CTNAG 7 cut(s) 39, 852, 1545, 1671, 1821, 2398, 2861
HpySE526I ACGT 6 cut(s) 612, 922, 1016, 1636, 2661, 2892
HspAI GCGC 2 cut(s) 154, 2609
Kpn2I TCCGGA 1 cut(s) 2922
Ksp22I TGATCA 2 cut(s) 211, 1984
LguI GCTCTTC 2 cut(s) 900, 2904
LmnI GCTCC 8 cut(s) 725, 756, 853, 873, 1843, 2150, 2296, 2314
Lsp1109I GCAGC 7 cut(s) 889, 917, 1049, 1156, 2443, 2611, 2745
LweI GCATC 5 cut(s) 769, 917, 1810, 2384, 2578
MabI ACCWGGT 1 cut(s) 1123
MaeII ACGT 6 cut(s) 612, 922, 1016, 1636, 2661, 2892
MaeIII GTNAC 6 cut(s) 791, 1027, 1061, 2099, 2319, 2834
MfeI CAATTG 3 cut(s) 65, 813, 2600
MflI RGATCY 5 cut(s) 370, 968, 1162, 1769, 2941
MhlI GDGCHC 3 cut(s) 850, 878, 922
MlyI GAGTC 7 cut(s) 1178, 1241, 1260, 2272, 2272, 2390, 2589
MmeI TCCRAC 5 cut(s) 322, 478, 1140, 1578, 2479
Mph1103I ATGCAT 1 cut(s) 601
MroI TCCGGA 1 cut(s) 2922
MroXI GAANNNNTTC 2 cut(s) 372, 1095
MseI TTAA 4 cut(s) 354, 926, 1587, 2883
MslI CAYNNNNRTG 1 cut(s) 1296
MspA1I CMGCKG 2 cut(s) 2758, 2901
MspI CCGG 6 cut(s) 1735, 2214, 2298, 2331, 2923, 2931
MunI CAATTG 3 cut(s) 65, 813, 2600
Mva1269I GAATGC 2 cut(s) 1846, 2458
MvaI CCWGG 6 cut(s) 1125, 1389, 1930, 2244, 2656, 2937
MwoI GCNNNNNNNGC 8 cut(s) 70, 84, 566, 899, 953, 1144, 1346, 2492
NciI CCSGG 4 cut(s) 1736, 2298, 2331, 2931
NdeI CATATG 3 cut(s) 178, 493, 2695
NlaIV GGNNCC 4 cut(s) 113, 1164, 1593, 1771
NmeAIII GCCGAG 1 cut(s) 2038
NmuCI GTSAC 1 cut(s) 791
NsiI ATGCAT 1 cut(s) 601
NspI RCATGY 4 cut(s) 477, 573, 621, 763
OliI CACNNNNGTG 1 cut(s) 1296
PaeI GCATGC 2 cut(s) 621, 763
PaeR7I CTCGAG 2 cut(s) 1463, 2707
PasI CCCWGGG 1 cut(s) 1929
PceI AGGCCT 1 cut(s) 1543
PciSI GCTCTTC 2 cut(s) 900, 2904
PctI GAATGC 2 cut(s) 1846, 2458
PdmI GAANNNNTTC 2 cut(s) 372, 1095
PfeI GAWTC 5 cut(s) 1700, 1757, 1937, 2006, 2069
PflMI CCANNNNNTGG 4 cut(s) 121, 869, 2605, 2831
PfoI TCCNGGA 3 cut(s) 2242, 2296, 2935
PkrI GCNGC 8 cut(s) 170, 904, 907, 1039, 1146, 2458, 2626, 2760
PleI GAGTC 7 cut(s) 1177, 1240, 1259, 2271, 2272, 2390, 2589
PpsI GAGTC 7 cut(s) 1177, 1240, 1259, 2271, 2272, 2390, 2589
Psp124BI GAGCTC 1 cut(s) 878
Psp1406I AACGTT 1 cut(s) 2892
Psp6I CCWGG 6 cut(s) 1123, 1387, 1928, 2242, 2654, 2935
PspEI GGTNACC 1 cut(s) 2319
PspFI CCCAGC 1 cut(s) 2853
PspGI CCWGG 6 cut(s) 1123, 1387, 1928, 2242, 2654, 2935
PspN4I GGNNCC 4 cut(s) 113, 1164, 1593, 1771
PspPI GGNCC 4 cut(s) 1046, 1992, 2928, 2933
PspXI VCTCGAGB 1 cut(s) 2707
PsrI GAACNNNNNNTAC 2 cut(s) 997, 1029
PstI CTGCAG 2 cut(s) 907, 2461
PstNI CAGNNNCTG 2 cut(s) 1508, 1652
PsuI RGATCY 5 cut(s) 370, 968, 1162, 1769, 2941
PvuII CAGCTG 1 cut(s) 2758
RsaI GTAC 5 cut(s) 835, 1411, 1455, 1999, 2430
RsaNI GTAC 5 cut(s) 834, 1410, 1454, 1998, 2429
RseI CAYNNNNRTG 1 cut(s) 1296
SacI GAGCTC 1 cut(s) 878
SapI GCTCTTC 2 cut(s) 900, 2904
SaqAI TTAA 4 cut(s) 354, 926, 1587, 2883
SatI GCNGC 8 cut(s) 169, 903, 906, 1038, 1145, 2457, 2625, 2759
Sau96I GGNCC 4 cut(s) 1046, 1992, 2928, 2933
ScaI AGTACT 2 cut(s) 835, 1999
SchI GAGTC 7 cut(s) 1178, 1241, 1260, 2272, 2272, 2390, 2589
SduI GDGCHC 3 cut(s) 850, 878, 922
SexAI ACCWGGT 1 cut(s) 1123
SfaNI GCATC 5 cut(s) 769, 917, 1810, 2384, 2578
SfcI CTRYAG 3 cut(s) 903, 1020, 2457
Sfr274I CTCGAG 2 cut(s) 1463, 2707
SinI GGWCC 1 cut(s) 2933
SlaI CTCGAG 2 cut(s) 1463, 2707
SmiMI CAYNNNNRTG 1 cut(s) 1296
SmlI CTYRAG 5 cut(s) 660, 1085, 1463, 2280, 2707
SmoI CTYRAG 5 cut(s) 660, 1085, 1463, 2280, 2707
SpeI ACTAGT 1 cut(s) 2237
SphI GCATGC 2 cut(s) 621, 763
SseBI AGGCCT 1 cut(s) 1543
SsiI CCGC 2 cut(s) 169, 2901
SspI AATATT 1 cut(s) 392
SstI GAGCTC 1 cut(s) 878
StuI AGGCCT 1 cut(s) 1543
TaiI ACGT 6 cut(s) 615, 925, 1019, 1639, 2664, 2895
TaqI TCGA 7 cut(s) 266, 425, 517, 1320, 1464, 1664, 2708
TatI WGTACW 3 cut(s) 833, 1453, 1997
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 5 cut(s) 1700, 1757, 1937, 2006, 2069
Tru1I TTAA 4 cut(s) 354, 926, 1587, 2883
Tru9I TTAA 4 cut(s) 354, 926, 1587, 2883
TscAI CASTG 7 cut(s) 484, 567, 727, 796, 1404, 1524, 2807
TseFI GTSAC 1 cut(s) 791
TseI GCWGC 7 cut(s) 902, 905, 1037, 1144, 2456, 2624, 2758
Tsp45I GTSAC 1 cut(s) 791
TspGWI ACGGA 3 cut(s) 2732, 2887, 2934
TspRI CASTG 7 cut(s) 484, 567, 727, 796, 1404, 1524, 2807
Van91I CCANNNNNTGG 4 cut(s) 121, 869, 2605, 2831
VpaK11BI GGWCC 1 cut(s) 2933
XapI RAATTY 7 cut(s) 1095, 1279, 1369, 1527, 2446, 2572, 2918
XceI RCATGY 4 cut(s) 477, 573, 621, 763
XhoI CTCGAG 2 cut(s) 1463, 2707
XmiI GTMKAC 2 cut(s) 483, 1019
XmnI GAANNNNTTC 2 cut(s) 372, 1095
ZrmI AGTACT 2 cut(s) 835, 1999
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.