MD03G1108000.v1.1

RING/Ubox like zinc-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001311
Physical Location & Seq
Reverse (-)
9364246 .. 9373596
9351 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1108000.v1.1.491

Sequence Viewer

Length: 3084 bp
ATGGATTTGACAGATCAGCAATTGAAGCCATGCAAATGTGGTTATGAGATATGTGTTTGGTGTTGGCACCACATAATGGACATGGCTGAGAAGGATGAGACAGAGGGGCGGTGCCCTGCATGCCGCACTCCTTATGACAAGGAAAAGATTGTCGGAACGGCTGGAAAGTGTGTGAGGCTGGTGGCAGAAATCAACACTGAGAAAAAGATGAAGTCTCAGAAGGCAAAGGTCAAATCTACTGAAGGGCGGAAGCAGCTGACTAGCGTGCGAGTAATTCAACGGAACCTCGTTTACATAGTTGGGTTGCCTCTTAATCTGGCAGATGAAGATCTTCTGCAGCGCAGAGAATATTTTGGTCAGTATGGGAAGGTTCAAAAAGTGTCTATGTCTCGAACAGCAGCTGGCGTCATTCAGCAATTTCCAAACAATACATGTAGTGTATATATTACATACTTGAAAGAGGAGGAAGCGATCCGTTGTATTCAAAATGTACATGGGTTCCTCTTGGATGGTAGATCTTTGAGGGCTTGCTTTGGGACCACAAAATATTGTCATGCATGGCTGAGGAATGTGCCTTGCACCAATCCTGATTGTTTGTATCTGCACGAGGTTGGGTCTCAGGAGGATAGTTTCACTAAAGATGAAATAATTTCTGCATACACTAGGGTACAACAAATTACTGGTACAGAAAACAGTATGCAACGGCGTTCAGGGAGTGTTTTGCCACCACCACTAGATGATTATTGCAACACCAGTTCTGCTTCTGCTGCAGGACCAATTATTAGAAATGGTTCAAGTAATACTGAAAGTCTTATTAGAGGTTCTCCTCCAAATGGAAGCTCTGGTAGATCTATTGCTCTTCCAGCTGCAGCCTCATGGGGAACACGTGGTTCAAACTGCCAACCACCAGCTACACATATAATAAGTTCAAATGGACATCCAAAACAGAAACCTGATACTGTCAGTTGCATGCTGCCATTTTCTTCAGCAGCTGTTGCTTCTGTTCAGTCATCTACAGTGCATAATGATGCTGGAAAGAGGTCAGCATTAAATGAAGAAAGTCAGGCTGTGCATGCTAAATCTAAACCAGAATCATTGAAGATTGTGAAACAGCGTAGTGGTGTGGATTGTGAAAATGATCTGTCTGACAAACCTGCTGCACCCAATGAAGGCTCTGCTTCTGTGAATGTAGACAGCCAGTTATCTGCCCCATCAGTATCCAAGGACAATGATAGAGGCAGTAGCATGCAGGCAAACATTAGCAATCCCACCAACTATAATCATCTATCTTACAGTTCTCGTCATGAAAAAGAAAACATTTTTTCTGCTGAAGAAGTGGTCCAAAACTTGTGCTCTGATATCCCTCTAATGAGCATTGATAGAAATGCCAAGGTTGAACATTCCAGTGTAGTCAGACCTAATAGTTCCCTTTCAGATAACTCCTTTATTAAATCGCCTCGGAACCAACAATATTGTGCTGAGCAATCTAGAGAGCCTCCAACAACTGGTGAGAAAGCTGTCACACCTGTTAATGGGGTGTGTGTTACAAGGGAACAATCCAACTGGACCCTAGATTCGCAAGCCCATTTAGTGCCAAGTACATCTTCAGAAGTGGAAGAGGATGTACTATCTTTTGACAATCAAAGACTCAAGGATCCAGAAGTGAGTCGGTCAACATATTTGCCTAGTTTGCCTAATACAGTACATGCTCCTAACCACTCCAGGTCTCCTTTGTTGCATAATGAGGCTTATGGTGCAGTTTATTCCAATGCTGATCGTCTATTTGTAGATAACAAAGTTAGGGACAGTTCACTTCTATCTTCGTCCAGTGTTTCAATAACATCTAATGGATACCCTGAAAACATGGTAACCAGATCTTCTGGTTCAGAGAGGCCATTGGAGCATTCCTATCCTCTTCTAAATGACATCCCAGGGAAGCACACTGGAAGATTCCTGGATGATGCAGCAAATCCTGATTTCAGTACTGCTGTTGACAAGGGAGAGAGTAGCATAATCTCAAATATATTATCGATGGACTCACTAACATGGGATGACTCACTAACATCACCACAGCATTTCTCCAAATTCTTGGGTGAAACTGATAGACAGTCTGGAGCTCTCAAAATGTCTAGTCCTTGGAAAGTACAAAATAACAATCAGTCCAGATTCTCTTTTGCAAGACAGGAGGATGCTAAAAATCAAGCATTTGATGTACAGTCATCTCTGAATGTTGGCGGGCAGTTTTCTAATAACCAGTCATTCCACCAAGGCTTTTCTGATAATAGAGATTTATTTTTGGATAATCTAGGAATTGGCAATGGTTTCCCATCTAGTACTTTTGAAGAATCTGAAAATCATGCCAGCAATCATTTAGCTTTTTCTTCTAACAAGCTTTCTGCGGTTTCAAGGGCTCAAATTTCTGCTCCTCCTGGATTCTCTGTGCCAAGCAGGGCACCACCTCCGGGCTTTACTTCTCATGAGAGAGTGGATCAGGACTTTGACACTCTGTCTGGGAATCATCTGTATGATAATTCATCTTTGTTGAGGAACACATATCAACCTTGGGCAACTGGAAATATTGGGAGCTCCGCAGATATTGAGTTTATGGATCCTGCTATATTGGCAGTTGGAAAGGGGAGACTGCAAGGTGGGCTTAACAACCAAGGCCTAGAAATGAGATCAAACTTCCCTTCGCAGTTAAGTGGGTATGAAAATGATGCAAGGCTTCAACTATTGATGCAAAGATCACTGGCCCCACAACAAAATCTTAGATTTCCTGATTTTGGGGATGGTTTTTCTCACGTCAATGATTCTTATGGTTTCTCCTCAAGGCGTTTGGAGCAGTCCCAGGCTAGCAATCTTTCTCCTTTTTCGCAAATGTCCCTTCAACAGTCCAGAAACCGGGGCATGTCAAATGGCCACTGGGATGGGTGGAATGAGGTTCAGGGTGGAAGCAATGTAGGTATGTCAGAGCTCCTGAGAAATGAGAGACTAGGATTCAACAAGTTTTACTCTGGTTATGAAGAATCAAAGTTTCGGATGCCCAGTTCCGGCGATCTATATAACAGAACATTTGGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0000288 GO:0000289 GO:0000956 GO:0002682 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006977 GO:0007049 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010468 GO:0010564 GO:0010605 GO:0010629 GO:0010948 GO:0016070 GO:0016071 GO:0016567 GO:0016740 GO:0019222 GO:0019439 GO:0019538 GO:0019787 GO:0019941 GO:0022402 GO:0023052 GO:0030163 GO:0030330 GO:0031570 GO:0031571 GO:0032446 GO:0033554 GO:0034641 GO:0034655 GO:0035556 GO:0036211 GO:0042770 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045595 GO:0045637 GO:0045652 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0046700 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051603 GO:0051716 GO:0051726 GO:0051865 GO:0060255 GO:0065007 GO:0070647 GO:0071156 GO:0071158 GO:0071704 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0090068 GO:0090304 GO:0140096 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1903706 GO:2000026 GO:2000045 GO:2000134
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
RRM_1 zf-RING_4

Protein Analysis

1028

Amino Acids

113.05

Weight (kDa)

6.14

Isoelectric Point (pI)

55.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_4 PF14570 1 - 46 2.7e-16 RING/Ubox like zinc-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1162
AccB1I GGYRCC 3 cut(s) 66, 111, 2452
AccB7I CCANNNNNTGG 2 cut(s) 76, 1505
AccI GTMKAC 1 cut(s) 1191
AciI CCGC 6 cut(s) 109, 124, 247, 2235, 2399, 2589
AclWI GGATC 6 cut(s) 466, 1649, 1662, 2496, 2603, 2616
AcoI YGGCCR 1 cut(s) 2917
AcsI RAATTY 2 cut(s) 2084, 2415
AcuI CTGAAG 4 cut(s) 261, 969, 1350, 1590
AcvI CACGTG 1 cut(s) 887
AcyI GRCGYC 1 cut(s) 405
AfiI CCNNNNNNNGG 9 cut(s) 76, 833, 1169, 1505, 1532, 2462, 2783, 2901, 3050
AflIII ACRYGT 2 cut(s) 431, 884
AhdI GACNNNNNGTC 1 cut(s) 2506
AjiI CACGTC 1 cut(s) 2803
AjnI CCWGG 5 cut(s) 1721, 1930, 1953, 2428, 2847
AjuI GAANNNNNNNTTGG 2 cut(s) 1588, 1620
AloI GAACNNNNNNTCC 2 cut(s) 3031, 3063
Alw21I GWGCWC 4 cut(s) 1355, 2119, 2588, 2976
Alw26I GTCTC 7 cut(s) 92, 219, 393, 621, 1731, 2632, 2983
AlwI GGATC 6 cut(s) 466, 1649, 1662, 2496, 2603, 2616
AlwNI CAGNNNCTG 2 cut(s) 401, 992
AoxI GGCC 4 cut(s) 1892, 2665, 2751, 2917
ApoI RAATTY 2 cut(s) 2084, 2415
ArsI GACNNNNNNTTYG 2 cut(s) 1558, 1590
Asp700I GAANNNNTTC 2 cut(s) 330, 790
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 5 cut(s) 537, 773, 1339, 1566, 2752
AsuC2I CCSGG 2 cut(s) 2463, 2903
AsuHPI GGTGA 3 cut(s) 1520, 2058, 2105
AsuNHI GCTAGC 1 cut(s) 2852
AvaII GGWCC 4 cut(s) 537, 773, 1339, 1566
BaeGI GKGCMC 2 cut(s) 116, 2455
BalI TGGCCA 1 cut(s) 2919
BamHI GGATCC 2 cut(s) 1654, 2608
BanI GGYRCC 3 cut(s) 66, 111, 2452
BanII GRGCYC 4 cut(s) 2119, 2413, 2588, 2976
BarI GAAGNNNNNNTAC 2 cut(s) 1608, 1640
BauI CACGAG 1 cut(s) 605
BbrPI CACGTG 1 cut(s) 887
Bbv12I GWGCWC 4 cut(s) 1355, 2119, 2588, 2976
BbvCI CCTCAGC 1 cut(s) 563
BccI CCATC 6 cut(s) 503, 1219, 2026, 2335, 2783, 2921
BceAI ACGGC 2 cut(s) 174, 719
BciT130I CCWGG 5 cut(s) 1723, 1932, 1955, 2430, 2849
BciVI GTATCC 2 cut(s) 1228, 1844
BcnI CCSGG 2 cut(s) 2463, 2903
BcoDI GTCTC 7 cut(s) 92, 219, 393, 621, 1731, 2632, 2983
BfmI CTRYAG 4 cut(s) 335, 768, 867, 1014
BfuAI ACCTGC 1 cut(s) 1162
BfuI GTATCC 2 cut(s) 1228, 1844
BglII AGATCT 4 cut(s) 328, 515, 848, 1874
BlpI GCTNAGC 1 cut(s) 1479
BmcAI AGTACT 2 cut(s) 1984, 2335
Bme1390I CCNGG 7 cut(s) 1723, 1932, 1955, 2430, 2463, 2849, 2903
Bme18I GGWCC 4 cut(s) 537, 773, 1339, 1566
BmeRI GACNNNNNGTC 1 cut(s) 2506
BmgBI CACGTC 1 cut(s) 2803
BmgT120I GGNCC 5 cut(s) 537, 773, 1339, 1566, 2752
BmrFI CCNGG 7 cut(s) 1723, 1932, 1955, 2430, 2463, 2849, 2903
BmrI ACTGGG 2 cut(s) 2932, 3039
BmsI GCATC 6 cut(s) 1018, 1951, 2179, 2707, 2727, 3030
BmtI GCTAGC 1 cut(s) 2856
BmuI ACTGGG 2 cut(s) 2932, 3039
BpmI CTGGAG 2 cut(s) 1705, 2133
Bpu10I CCTNAGC 1 cut(s) 563
Bpu1102I GCTNAGC 1 cut(s) 1479
BpuEI CTTGAG 2 cut(s) 1634, 2812
BpuMI CCSGG 2 cut(s) 2463, 2903
Bsa29I ATCGAT 1 cut(s) 2030
BsaAI YACGTR 1 cut(s) 887
BsaBI GATNNNNATC 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 405
BsaI GGTCTC 2 cut(s) 621, 1731
Bsc4I CCNNNNNNNGG 9 cut(s) 76, 833, 1169, 1505, 1532, 2462, 2783, 2901, 3050
Bse3DI GCAATG 2 cut(s) 2323, 2962
Bse8I GATNNNNATC 1 cut(s) 327
BseBI CCWGG 5 cut(s) 1723, 1932, 1955, 2430, 2849
BseCI ATCGAT 1 cut(s) 2030
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 9 cut(s) 76, 833, 1169, 1505, 1532, 2462, 2783, 2901, 3050
BseMI GCAATG 2 cut(s) 2323, 2962
BseMII CTCAG 7 cut(s) 78, 189, 230, 554, 632, 1470, 2969
BseRI GAGGAG 4 cut(s) 476, 816, 2415, 2815
BseSI GKGCMC 2 cut(s) 116, 2455
BsgI GTGCAG 3 cut(s) 587, 1143, 1776
BshFI GGCC 4 cut(s) 1894, 2667, 2753, 2919
BshNI GGYRCC 3 cut(s) 66, 111, 2452
BshVI ATCGAT 1 cut(s) 2030
BsiHKAI GWGCWC 4 cut(s) 1355, 2119, 2588, 2976
BsiSI CCGG 3 cut(s) 2462, 2902, 3051
BslFI GGGAC 4 cut(s) 550, 1817, 2830, 2866
BslI CCNNNNNNNGG 9 cut(s) 76, 833, 1169, 1505, 1532, 2462, 2783, 2901, 3050
BsmAI GTCTC 7 cut(s) 92, 219, 393, 621, 1731, 2632, 2983
BsmFI GGGAC 4 cut(s) 550, 1817, 2830, 2866
BsmI GAATGC 1 cut(s) 1903
BsnI GGCC 4 cut(s) 1894, 2667, 2753, 2919
Bso31I GGTCTC 2 cut(s) 621, 1731
Bsp1286I GDGCHC 7 cut(s) 116, 1355, 2119, 2413, 2455, 2588, 2976
Bsp1407I TGTACA 2 cut(s) 490, 2212
Bsp1720I GCTNAGC 1 cut(s) 1479
BspACI CCGC 6 cut(s) 109, 124, 247, 2235, 2399, 2589
BspANI GGCC 4 cut(s) 1894, 2667, 2753, 2919
BspCNI CTCAG 7 cut(s) 79, 190, 229, 555, 631, 1471, 2970
BspDI ATCGAT 1 cut(s) 2030
BspHI TCATGA 2 cut(s) 1303, 2476
BspMAI CTGCAG 3 cut(s) 339, 772, 871
BspMI ACCTGC 1 cut(s) 1162
BspOI GCTAGC 1 cut(s) 2856
BspPI GGATC 6 cut(s) 466, 1649, 1662, 2496, 2603, 2616
BspQI GCTCTTC 1 cut(s) 864
BspT107I GGYRCC 3 cut(s) 66, 111, 2452
BspTNI GGTCTC 2 cut(s) 621, 1731
BsrDI GCAATG 2 cut(s) 2323, 2962
BsrGI TGTACA 2 cut(s) 490, 2212
BssNI GRCGYC 1 cut(s) 405
BssSI CACGAG 1 cut(s) 605
BssT1I CCWWGG 6 cut(s) 1221, 1389, 2135, 2266, 2561, 2662
Bst2BI CACGAG 1 cut(s) 605
Bst2UI CCWGG 5 cut(s) 1723, 1932, 1955, 2430, 2849
Bst4CI ACNGT 9 cut(s) 695, 961, 1018, 1295, 1702, 1808, 2109, 2217, 2892
Bst6I CTCTTC 3 cut(s) 864, 1611, 1920
BstACI GRCGYC 1 cut(s) 405
BstAPI GCANNNNNTGC 1 cut(s) 995
BstAUI TGTACA 2 cut(s) 490, 2212
BstBAI YACGTR 1 cut(s) 887
BstDEI CTNAG 8 cut(s) 87, 198, 216, 563, 618, 1479, 2768, 2978
BstEII GGTNACC 1 cut(s) 1867
BstHHI GCGC 1 cut(s) 342
BstMAI GTCTC 7 cut(s) 92, 219, 393, 621, 1731, 2632, 2983
BstNI CCWGG 5 cut(s) 1723, 1932, 1955, 2430, 2849
BstNSI RCATGY 7 cut(s) 123, 435, 973, 1076, 1249, 1709, 2911
BstPI GGTNACC 1 cut(s) 1867
BstSCI CCNGG 7 cut(s) 1721, 1930, 1953, 2428, 2461, 2847, 2901
BstSFI CTRYAG 4 cut(s) 335, 768, 867, 1014
BstSLI GKGCMC 2 cut(s) 116, 2455
BstX2I RGATCY 6 cut(s) 328, 515, 848, 1654, 1874, 2608
BstXI CCANNNNNNTGG 2 cut(s) 2089, 2927
BstYI RGATCY 6 cut(s) 328, 515, 848, 1654, 1874, 2608
Bsu15I ATCGAT 1 cut(s) 2030
BsuI GTATCC 2 cut(s) 1228, 1844
BsuRI GGCC 4 cut(s) 1894, 2667, 2753, 2919
BsuTUI ATCGAT 1 cut(s) 2030
BtrI CACGTC 1 cut(s) 2803
BtsIMutI CAGTG 7 cut(s) 195, 1023, 1411, 1834, 1941, 2747, 2920
BveI ACCTGC 1 cut(s) 1162
CaiI CAGNNNCTG 2 cut(s) 401, 992
CciI TCATGA 2 cut(s) 1303, 2476
CfoI GCGC 1 cut(s) 342
Cfr13I GGNCC 5 cut(s) 537, 773, 1339, 1566, 2752
ClaI ATCGAT 1 cut(s) 2030
CseI GACGC 1 cut(s) 394
DdeI CTNAG 8 cut(s) 87, 198, 216, 563, 618, 1479, 2768, 2978
DriI GACNNNNNGTC 1 cut(s) 2506
EaeI YGGCCR 1 cut(s) 2917
Eam1104I CTCTTC 3 cut(s) 864, 1611, 1920
Eam1105I GACNNNNNGTC 1 cut(s) 2506
EarI CTCTTC 3 cut(s) 864, 1611, 1920
EciI GGCGGA 1 cut(s) 262
Ecl136II GAGCTC 3 cut(s) 2117, 2586, 2974
Eco130I CCWWGG 6 cut(s) 1221, 1389, 2135, 2266, 2561, 2662
Eco147I AGGCCT 1 cut(s) 2667
Eco24I GRGCYC 4 cut(s) 2119, 2413, 2588, 2976
Eco31I GGTCTC 2 cut(s) 621, 1731
Eco32I GATATC 1 cut(s) 1360
Eco47I GGWCC 4 cut(s) 537, 773, 1339, 1566
Eco53kI GAGCTC 3 cut(s) 2117, 2586, 2974
Eco57I CTGAAG 4 cut(s) 261, 969, 1350, 1590
Eco72I CACGTG 1 cut(s) 887
Eco91I GGTNACC 1 cut(s) 1867
EcoICRI GAGCTC 3 cut(s) 2117, 2586, 2974
EcoO65I GGTNACC 1 cut(s) 1867
EcoRII CCWGG 5 cut(s) 1721, 1930, 1953, 2428, 2847
EcoRV GATATC 1 cut(s) 1360
EcoT14I CCWWGG 6 cut(s) 1221, 1389, 2135, 2266, 2561, 2662
EcoT22I ATGCAT 1 cut(s) 559
EcoT38I GRGCYC 4 cut(s) 2119, 2413, 2588, 2976
ErhI CCWWGG 6 cut(s) 1221, 1389, 2135, 2266, 2561, 2662
FalI AAGNNNNNCTT 4 cut(s) 1588, 1620, 2637, 2669
FaqI GGGAC 4 cut(s) 550, 1817, 2830, 2866
FauI CCCGC 1 cut(s) 2228
FblI GTMKAC 1 cut(s) 1191
FriOI GRGCYC 4 cut(s) 2119, 2413, 2588, 2976
GlaI GCGC 1 cut(s) 341
GsuI CTGGAG 2 cut(s) 1705, 2133
HaeIII GGCC 4 cut(s) 1894, 2667, 2753, 2919
HapII CCGG 3 cut(s) 2462, 2902, 3051
HgaI GACGC 1 cut(s) 394
HhaI GCGC 1 cut(s) 342
Hin1I GRCGYC 1 cut(s) 405
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HincII GTYRAC 2 cut(s) 1674, 1993
HindII GTYRAC 2 cut(s) 1674, 1993
HindIII AAGCTT 1 cut(s) 2390
HpaII CCGG 3 cut(s) 2462, 2902, 3051
HphI GGTGA 3 cut(s) 1520, 2058, 2105
Hpy166II GTNNAC 5 cut(s) 292, 1192, 1674, 1811, 1993
Hpy8I GTNNAC 5 cut(s) 292, 1192, 1674, 1811, 1993
HpyAV CCTTC 7 cut(s) 85, 214, 236, 361, 1163, 2700, 2894
HpyCH4III ACNGT 9 cut(s) 695, 961, 1018, 1295, 1702, 1808, 2109, 2217, 2892
HpyCH4IV ACGT 2 cut(s) 886, 2802
HpyF3I CTNAG 8 cut(s) 87, 198, 216, 563, 618, 1479, 2768, 2978
HpySE526I ACGT 2 cut(s) 886, 2802
Hsp92I GRCGYC 1 cut(s) 405
HspAI GCGC 1 cut(s) 340
LguI GCTCTTC 1 cut(s) 864
LmnI GCTCC 8 cut(s) 1714, 1900, 2114, 2428, 2583, 2591, 2839, 2979
LweI GCATC 6 cut(s) 1018, 1951, 2179, 2707, 2727, 3030
MaeII ACGT 2 cut(s) 886, 2802
MaeIII GTNAC 3 cut(s) 1519, 1543, 1867
MfeI CAATTG 1 cut(s) 20
MflI RGATCY 6 cut(s) 328, 515, 848, 1654, 1874, 2608
MhlI GDGCHC 7 cut(s) 116, 1355, 2119, 2413, 2455, 2588, 2976
MlsI TGGCCA 1 cut(s) 2919
MluNI TGGCCA 1 cut(s) 2919
MlyI GAGTC 4 cut(s) 1641, 1675, 2030, 2048
MmeI TCCRAC 4 cut(s) 133, 1523, 1584, 2608
Mox20I TGGCCA 1 cut(s) 2919
Mph1103I ATGCAT 1 cut(s) 559
MroXI GAANNNNTTC 2 cut(s) 330, 790
MscI TGGCCA 1 cut(s) 2919
MseI TTAA 6 cut(s) 312, 1049, 1449, 1530, 2655, 2699
MslI CAYNNNNRTG 7 cut(s) 34, 1026, 1404, 2044, 2523, 2805, 2925
Msp20I TGGCCA 1 cut(s) 2919
MspA1I CMGCKG 4 cut(s) 256, 401, 866, 992
MspI CCGG 3 cut(s) 2462, 2902, 3051
MspR9I CCNGG 7 cut(s) 1723, 1932, 1955, 2430, 2463, 2849, 2903
MunI CAATTG 1 cut(s) 20
Mva1269I GAATGC 1 cut(s) 1903
MvaI CCWGG 5 cut(s) 1723, 1932, 1955, 2430, 2849
NciI CCSGG 2 cut(s) 2463, 2903
NheI GCTAGC 1 cut(s) 2852
NmuCI GTSAC 1 cut(s) 1519
NsiI ATGCAT 1 cut(s) 559
NspI RCATGY 7 cut(s) 123, 435, 973, 1076, 1249, 1709, 2911
PaeI GCATGC 4 cut(s) 123, 973, 1076, 1249
PagI TCATGA 2 cut(s) 1303, 2476
PasI CCCWGGG 1 cut(s) 1931
PceI AGGCCT 1 cut(s) 2667
PciI ACATGT 1 cut(s) 431
PciSI GCTCTTC 1 cut(s) 864
PctI GAATGC 1 cut(s) 1903
PdmI GAANNNNTTC 2 cut(s) 330, 790
PflMI CCANNNNNTGG 2 cut(s) 76, 1505
PfoI TCCNGGA 2 cut(s) 1953, 2428
PleI GAGTC 4 cut(s) 1641, 1674, 2030, 2048
PmaCI CACGTG 1 cut(s) 887
PmlI CACGTG 1 cut(s) 887
PpsI GAGTC 4 cut(s) 1641, 1674, 2030, 2048
Ppu21I YACGTR 1 cut(s) 887
PscI ACATGT 1 cut(s) 431
Psp124BI GAGCTC 3 cut(s) 2119, 2588, 2976
Psp6I CCWGG 5 cut(s) 1721, 1930, 1953, 2428, 2847
PspCI CACGTG 1 cut(s) 887
PspEI GGTNACC 1 cut(s) 1867
PspGI CCWGG 5 cut(s) 1721, 1930, 1953, 2428, 2847
PspPI GGNCC 5 cut(s) 537, 773, 1339, 1566, 2752
PsrI GAACNNNNNNTAC 2 cut(s) 275, 307
PstI CTGCAG 3 cut(s) 339, 772, 871
PstNI CAGNNNCTG 2 cut(s) 401, 992
PsuI RGATCY 6 cut(s) 328, 515, 848, 1654, 1874, 2608
PvuII CAGCTG 4 cut(s) 256, 401, 866, 992
RseI CAYNNNNRTG 7 cut(s) 34, 1026, 1404, 2044, 2523, 2805, 2925
SacI GAGCTC 3 cut(s) 2119, 2588, 2976
SapI GCTCTTC 1 cut(s) 864
SaqAI TTAA 6 cut(s) 312, 1049, 1449, 1530, 2655, 2699
Sau96I GGNCC 5 cut(s) 537, 773, 1339, 1566, 2752
ScaI AGTACT 2 cut(s) 1984, 2335
SchI GAGTC 4 cut(s) 1641, 1675, 2030, 2048
ScrFI CCNGG 7 cut(s) 1723, 1932, 1955, 2430, 2463, 2849, 2903
SduI GDGCHC 7 cut(s) 116, 1355, 2119, 2413, 2455, 2588, 2976
SfaNI GCATC 6 cut(s) 1018, 1951, 2179, 2707, 2727, 3030
SfcI CTRYAG 4 cut(s) 335, 768, 867, 1014
SinI GGWCC 4 cut(s) 537, 773, 1339, 1566
SmiMI CAYNNNNRTG 7 cut(s) 34, 1026, 1404, 2044, 2523, 2805, 2925
SmlI CTYRAG 2 cut(s) 1649, 2827
SmoI CTYRAG 2 cut(s) 1649, 2827
SphI GCATGC 4 cut(s) 123, 973, 1076, 1249
SseBI AGGCCT 1 cut(s) 2667
SsiI CCGC 6 cut(s) 109, 124, 247, 2235, 2399, 2589
SspI AATATT 4 cut(s) 350, 548, 1472, 2578
SstI GAGCTC 3 cut(s) 2119, 2588, 2976
StuI AGGCCT 1 cut(s) 2667
StyD4I CCNGG 7 cut(s) 1721, 1930, 1953, 2428, 2461, 2847, 2901
StyI CCWWGG 6 cut(s) 1221, 1389, 2135, 2266, 2561, 2662
TaaI ACNGT 9 cut(s) 695, 961, 1018, 1295, 1702, 1808, 2109, 2217, 2892
TaiI ACGT 2 cut(s) 889, 2805
TaqI TCGA 2 cut(s) 391, 2030
TaqII GACCGA 1 cut(s) 1659
TatI WGTACW 8 cut(s) 490, 1598, 1624, 1702, 1982, 2143, 2212, 2333
TauI GCSGC 1 cut(s) 126
Tru1I TTAA 6 cut(s) 312, 1049, 1449, 1530, 2655, 2699
Tru9I TTAA 6 cut(s) 312, 1049, 1449, 1530, 2655, 2699
TscAI CASTG 7 cut(s) 202, 1023, 1411, 1834, 1948, 2754, 2927
TseFI GTSAC 1 cut(s) 1519
Tsp45I GTSAC 1 cut(s) 1519
TspDTI ATGAA 9 cut(s) 224, 339, 657, 1068, 1182, 1320, 2523, 2724, 3036
TspGWI ACGGA 2 cut(s) 295, 464
TspRI CASTG 7 cut(s) 202, 1023, 1411, 1834, 1948, 2754, 2927
Van91I CCANNNNNTGG 2 cut(s) 76, 1505
VpaK11BI GGWCC 4 cut(s) 537, 773, 1339, 1566
XapI RAATTY 2 cut(s) 2084, 2415
XbaI TCTAGA 1 cut(s) 1487
XceI RCATGY 7 cut(s) 123, 435, 973, 1076, 1249, 1709, 2911
XmiI GTMKAC 1 cut(s) 1191
XmnI GAANNNNTTC 2 cut(s) 330, 790
ZrmI AGTACT 2 cut(s) 1984, 2335
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.