AT3G50380

SHR-binding domain of vacuolar-sorting associated protein 13

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004207
Physical Location & Seq
Reverse (-)
18686368 .. 18700710
14343 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50380.2

Sequence Viewer

Length: 9219 bp
ATGACGTTCCTTCGCAATTGGGTTCGACGGAGACTTCGTACTCTGTTACTCCCTTTTTCTCGCGATGAACCTGATCTTCAAGTCGAATTAGGGTTTACTGATACTCTCATCACTTTACGGAGTTTTCGATTCGATGTTTCTCAGCTTAATCAGCTTTTCGATGAATCGAATTTTCAATTTGAGAAATTTACTGTCGACCAATTAGTTGTTAGCTTTTCCGTTTGGTCAGCTCCGGCGATTAAATTCGAAATTCGCGGTGTCAATGTCAAATTGTCAGCTAGAGGGACGGACGAAGGAAGCTCGAGAAGGAAGCGAGCATCAAGTGATACTGTTGCTAATGAGATAAAGAAAGTTCTATCTTCTATTGATCCCAAGGGATGCGTCTTACATGACATTTTGGAAAAGATGCTTGGTCGTAGCACTTCCCAGATAAGTAAGCTCAAGACTTCATTCTCGAATCTTATTCTCAGGCATTTCCGTATTCAGATACATGGTATTAATGTACAAGTTTGTTTACCGGGTTCAAGTGACTTGAGTTGTCTTATGGAAATCAATGAGCTGAGGAGTGATTCTGAGAATTTTGGAAATCTTAGTTTAGTTAGGAGTTCGGCTGCTGCAGTTTTATTCCCTTTGAGGCGTAGTTCGTTTACGCTGAGTTGCTTTGGTTTTAACATTGGATATAAGAGGGATAATGAAATAGTTGACCTTTGTGGATTTGACAGCCTAGTCATGTTGATTACGCTGCATAATCTTCAACTTGTTGATCTGGTCGTCCGCGTTCCAGAGTTGAGCTTCTCGTTTAGACCCACTGATCTTCCGGTTTTAATGGGATTGGCGAATTTATCATCCAAAGATAGCAATTATGTCAGGAATGGACGTTATCTTTGGAAAGTTGCTGCTCGCAGAACTGGCTTGATGATTTCACCTCACAGTGTTTCATTCCAGAATTTAGTTAGTGTTGTTATCCTCTGGCTGCGATACGTAAATGCGTACGAGTATTTGCTATCTTTAGCTGGGTACTCCAGGAAAATGCCAGAGAAATCATTACTTTGGAAATTTTCAGAAAACAAAAGGCATTTTGTAACTGCAAGACGTAAATGGGAGATGATATGTAATATTGAGAAAGAGCTCCCTGCCGAAGCTATTGCACGGGCACGGAGAGTAGCCAGATACAGAGCTTGTCTGAATTCTCAGGATGCTGATGATGATTATGACGAATCTTCCCTTTACGGCCACTTCAAGTATTTGTCTAAGACCACCTGGGTTTTAGCTTATATCTGGAGACTTATAAGTAGAACATTCTGGTCTATAGCTTGCTTTCTGTGGTTAAACAAACTGTTGACCCAAGAACTACAAACCGATAGGAATAATGAAGATGATTCCGAGTGTGTATCTCTTGAGTTTCATGCAGTTGTAAATCTTGGGAAACTTTCTGTCACATGTTATCCAGAGAAAATAATTTCAAGTTTTATGACATCGAAAGACAGTACTGGACACGTGGACTCAAACATTGTCATGCTTTGCCTTTCGGTTGATGAGTTTTTGGTGTTGTATACTGTTGGCTGTCTTACTCAGTATCTGTCTGCTTCCTGTGGGAAACTGAAGGTTGAGTCTTCTAGTTTTAAGAATACCAGTCGTTTCATGAAATCTACAAAAGATCCCAGTAGTTCTTCTGAAGGAAATAAGAAGCACATGCGTGAAGATGTGAAAACTATTCTAGACATGGACCCAGCACAGCAAATTTCCAAAACAGTTAACAATCATGGCAGTGATCAACATGAAGGTATGCTGCATCTACAAAATCTTTTGAGAGAAATGTGGTTGAACTGGAACAGCAATTGCATGAAGTTAGATAAAAGTACTTTTACAATTTCTGATAAGCCTTGTTTACTGGTTGATATTAAAAGCTGTATGGCATATGAGGTTGTTGGTAACCAAGATTCCGAGTTCTGGAAATGCAGCATGGTGTTGGGAAAGTTGGATATTGTTTTTGAATATTCATCGTTGTTTTCATTGGCTTTGCTAATTTGGCAGATAGAATGGGCTCAGAAGTTACTTGTAGACGATTATACTGGAGAGGTGCATTCAAGTTCTTTGGTAACAGGCGGGGTTGATCCTGAAATGGCTAGTTATGACGAATATGGGATCTACAGGCGCAGTATTGAACTGTCTTTGCATAGAGTTCATCCAGAGAGACAGATTCAGGTTGGCATACTTCTTGGTGGCCCCCAGATCAAATTGTTAGTTGAGAAGGCTGAAGAAGTGAATACTTTAATTGGGAAGAAAGATATTCTATTATTTGATTTCCATGATTTTGAGTTTGTTATCTGGCCGACTTATAAATCTGACGTGGTGTCATCAAGGATGTTTCAAGGGCCTGATAACATAAGAACAGATAGGCCTTTGCTTCAGGAGCTGGGGCTAAGTGATACGGTGATTCCAAGTTATGAAAAATATGTTTCTCAAGGATGGAATTCCCTGTCCTCACACCTAAGGTTTTCTGGTTTTGATTGTTCTTTCTGCAAAATGGCAGTAAAGAACTGGAGTCAAGTTTTTGTAGTGAGACCCGTAACTATCTGCTTTTCCTCCTTAAGCGAAGCTATAATGGATTTCTCAATTGGTTTGGATGTGTTTGTGCTGGGTCTGACTATTGTAACAAAACCGGATGACCTAAATGCTTACTTTCAGATGCTTCTAAGTTTAGTTTCTGGGCTTTCCCGTGGTTTGAGTGGCTTAAGCTCTGGGGGTCATTCATCGGGACAAGAATTTCTCAGGTCTGATGCAGTGAATGTGGAACATGAGATCGAAAGGAATCCTTGCAAAACTCTTTTTGTAGTTAAAGCAAGCATTAAGCTGAAGGATATAGATGTGATATTTGATGTTCCTGCAGTAGACGACAAATTTGAGAGGCTTGTGGAGCTAGATGATACAAAAATCTGGTCTTCTGTCCAGGAAGCATGCATTGAACTATCTGTTGAAGAACATAAGTGTTTAATCAATGTTGATTTGTGTAAACTCCAATCAGTTCTCTTCAAATTTGAGGGTAATATATGGAAGAGTTCTGGCAACTTTATCACAGAATCTTTGCTGTTCAGGTCACATGATATCTTGTTTGAAGCATGCCTTTCTAGCTGCCTATTGAGTGTGTCTATGGATTGCTCAAGCCCATCAGCTCTAGGGGACGCATCAACAGCAAATGAACCTTCTACAAACAATGTTCAGGTTCAGAGAGAAGTAAAGACGTTGGACTCTGCTTCAGACCTTTTACCATCCAACTCAATCCGTTGGATGCACATCAATTTAGCATTGACTGACTTACTTGTAGCTAAAGGCTCAACAAAAAATGTCTTGGTGGACGTTCGTCGGTCAAGTAAGTTTGTAACATCGGTTTCTATTGGGAGAAGATTTCAGTCAATCTCCTGTAGCGTCGAGGGTGTTCTTTTTGTCCTTGAACCAAAGGCCTTGATAGGGTTAATTCATGGTTACTCCACGTACCTTTATTTAATTTCAAGTAAGGTGTCAGTGATTCAGAATTCTGCACCAGTTCTTGAGAAATTCGAAGCTGATTCAAGCGTCACTGAAATTAGTATTCCTTCTCAACAAGAAAACGGGTACCCTGTAGAAGCATTTTCTATAGATGTCGCCCAGTTTGCACTGGGTTTTGTGTGTGATGATGAATATGGAGCTATAAGGGAAATTGTCCTGGAGATCACTCTCCACAGTTCCCTTGACTCGGCAGGCGGAGAACAGAAGTTTCTTTGTGAGGTTTCTCGCCTGTCAGTTCTTTCAAAGATTCTTGAGAGCGTGGAAAGGGACATAAATATTACTCAATTTTCTTCTCCAGCATTTAGTAGCGAATCTTCTTCTTTTCTGCCTGGTACTCCTCTTGAAACATCATTTCAGCAAAGCGATGTAATCAGTTCAGGTGATAGCACAAGTGTTTCAGGAGATTTTAATTCTCTAAGAGAATTTTCTGCAAACAGCAATTTACAGGAAGAATTTCACTCTCGTTATAACAACTATATTTTGGAAGACCTACGTGTCTCTGCATCTGTTAAGAAGCGAGAAAATACCGGTCACCAGTTTAGCCAGGCATGGGCTGGCGCTTGTTCTGTTCTGGGATTTGATATAACTATTTCTCTGTCAGAATTGCAGATGGTACTTTCTATGCTTTCATTATTTGCTGCGATACCTGGAGGAGATAGCGCACATGCTTCTTTAGAAAGGCCTTCTTCGTTCAACAGTGAATCTGAAAGAAGTTTTGAGTCTGTAGTTCCTGATGGAGCGATTGTTGCTATTCAAGATATAAACCAACATATGTTCGTCACAGTGGAAGATGGGGGGAACAAATGTGTTGTGACTGGTACCCTTCATTATTCTCTTGTCGGAGAAAGAGCTCTTTTCAGGGTCAGCTACCACCGCCATCAAGGATGGAATTCATCCACCTTATGGTTTTCCTTGACATCTTTGTATGCCAAAAACAATAAAGGAGAGCCCCTAAGGTTAAACTATCATTCAAGTTCAGATATTGTAAACGTCTCTGGACTTTACGACAATGCACCAACACTCTTTCGGGCATCTTCTGGCGAATCCGAAAATTATAAGGGTGATATTGACTGGGAGACCTACCGTAAACTGGTCAAAGATACATTTTACCTCGTCAACAAGAAGAGTGATTCAGCTGTTGCATTTATTGATGGTTTTCCAGAATTTGTCCGGAAGCCAGGAAATCCATTCAAGTTTAAAGTGTTTCATGAAAGTTTGGCGACTCGTAGTCTTACACCTGTGGTACCCTCTGAGATCCATGAGTCTGAAACGCATTCTGTGATGGTGGACTCTTCTCCCCCTTCTATTACTGTAACAATTGATGGCGTATCTCTGACCATCGTCCATGAACTTTCAGAAACAAGGGACAGATTTCCCCTTTTTCGTGGTTCAGTCAATATCACACAGCTAACTGTACAAATGCTATCTTCTAAAGTCAGGATTATGAGCACATCAAACATATTAGTGCTGTACTTTGATGCTCAGACAAACCAATGGCGGGAATTTATTCATCCAGTTGAAGTTAGTGCTTTCTACCGTTCAACTTTTCAGACTCGGGATCTTAACAATACTATGCACAAAGTACCTACCCATATTTACTGCAGAATTGGAAAGTTGGAGGTGTTTCTAACCGAGCTGTCGTTGGATATGCTCCTTTTTTTGCTTGGAAAACTGGAGTTTGCTGGTCCATTTTCTGTGAAAACCTCCGCTATTCTTTCCAATTGTTGCAAGATAGAAAATCTCTCTGGCCTTGACCTTATTTGCCGTTTCAATGAGAAACAGACTGCCACAGTTGGCAGGAAGCAAACTGCTGCGATTTTTCTCCGGCATTCAATGAATCATCAACAAGAAGCTTCTCCAGTGGCTGCAGTCCAGCTATCTTCTGGAAAGTTCATAACTTCTTCTATTAACGTTTCTTTGTTAGAAGCTAGAACACTAGCTTGGAGAACAAGGATAATATCACTCCTAGATTCAAGGAGTCATCCTGGACCGTTTGTTGTTGTGGATATTAAGAAGGGACTTGAGGATGGTTTATCGATCTCAGTTTCTCCTTTGACAAGGATTCATAACGAGACCAGTCTTCCCATAGAGATACGTTTTCAACGATCTAAACAGAAGAGAGACGAATTTGCTTCTGTGCCTCTGAAGCCTGGTGGTTCAATTGATGATTCAGTCGCAGCATTTAATGCCATAAGCTCATCAGGAGATATGAAGAAGGCATTGACATCGTTAGCTGTTGGTAATTTTTCACTCTCTTTCAGACCTGAATCTTTCGAAACTTTGTTTGAGGGTGAGAAGTCTCTGGGAAGTGAATGGTCAGAAGAGCTTGAAGGAGGCAAGGCGGTACGCCTAACAGGAATTTTTGATAAATTAAGTTATGGAGTTAAAAAGGCTTTATCTATCGAATCAGTGAAGGTCTCCTTGACTACTACATATTGCTCTGTTACATCTGAAAGTCAGTGTGTTGGGAAGGTGCATTTTCTGATCCATAGCATTCGAAGGGAAGTATCGATCATACGGCCTGATGCATCCTCTGATGTACTTGAGAAACAGAAAGCATGTATTGCATTGCGAGAGCAAAAGGAAATTTTTCTTTTGCCCACTGTGCAGGTGTCGAATTTCCTTTCCTCCGAAGCAGCCATTTTATTGACAGAAACTGATCAGAACACCTCGATGGAGAGACACAGCATTGGCAAACATGCAACTATACAGAGCGGAAAAACAATTGACTTCTATGTTAATCCAGACATGATATACTTTAGAGTTACTCTCACTACTTCCCAAGCGAGCTGCAAACCAGTCAATAGTGGTCAGTGGGTCAAGAAGTTACAGAAACAGAAGAATGATGCACAATGTTTGGATGTTGATCTTGATTTTTCCGGCGGAAAATACTGTGCTTCCTTGAGATTGTCTTTAGGGAAAAGAGGCATACTGGAGGCAGCTGTTTTCACATCGTACATTCTTAAAAACGACTCAGACTGCACCTTGTTTTTCTTTCCCCCTGATCAGAAGCCTCTATCCAGAGAGGATATGGAAAAACTTGATCACATTGTCCCACCTGAGTTTGGATTGTATTTACCCCCAAAGACAGAGGGGTCGTGGTTTTTAAGATCTCGCAAGGTTGGTGTCATATTGGCTGATGGTCATGGGGCTACTGAAGCAGTGTTGGATTTGGATGCTTTATCAGGACTTACCGAAATTAGTTTGGGAACAAAAGATGAGTCTGGGTTTCGACATCTTGTAATCAATGAATCAGAAGAGACTATCAACATCCGTCAGCACTATTTTCAGGATGACTCTGTGGGCATAATCACCATCAAAAGCAAGCAGAGAGCAGCCTTGCGGTTGCAGGAAGAAACCATTCAGAAGAAAGAACTCCATCTGTTCGAAAACTTTATCAAAAAGCACGGAAGTGACAGTGCAAATTCATTGATATTCATTCAGTTCCGAAAGCAGTCAGGTGAAGCAGGCAGAGGCGCAATTGAATTTGCATCTGTTAATGTTACTGAAGAAGGTTCAACTCTTGCTGTGCACTTCCAAAAACCACCAAATACCCCACCACCATATCGCATTGAGAATTTCTTGCATTCTGCATCTCTCACGTACTACCAAAAGGATTCATCAGAGATTGAAGTACTTGGACCTGGAAGTGGGGCTGATTATGCATGGGATGATATGACACTTCCTCACAAGCTTGTAGTTATAGTAGATGGCATGATACCTCTGCGTGAAGTCAGCTTAGATAAGGTCCGTCCATGGAAACCCCTTTTCAAGGCAACTCAGCACAGAAGCATAGCCTCACACTTAATGATGAAAAAGAAGGCCAAAGATCATAAAACGGCTGACAAGGAATTGAGTAGAATCCCTATGGTAAAGGTCGGATATGAAGTCTATGCAGATGGCCTGACTCGAGTCATTCGCATTTGTGAAGTTTCAGAAAGCCTTAAAGGAGACTCAGCATTCCAATCTCGTTCGAAAATTCAATTCCGAGTAACCCATTTAGGAGTTCACTTACTTGAAAAAGTGAAGCAAAATGCAGAGGAGAAAACTGTCGTGTCTTATTCTCCGATCCTGGTGGCGAGACTTGAGAATGTTGGTCTTCATTCCATGTTCACTGACCAGCAAAAATTTAACCAATTATGTATAGAGGCTCTAAATGTGGATCACAAATGGGCAGGGGCACCCTTTGCAGCCATGCTTCGTCAGCATCAATCAAGTTCAAGTGACGCAAATGGTTGTCTATTCAAATGTGTATTCATTCTAGTATCAAGTGGTTCCAGCGTCACACAAGTCAAGCACTCCTCGATAGTTTTGCAGCCAGTCAATTTGAACCTAGACGAAGAGACTTTAATGAGAGTTGTGGCATTTTGGAGATCATCTCTCAGTACAAACACGCAAAGTAGTCAATATTATTTTGATCATTTTGAAATCCACCCAATAAAGATTACTGCTAATTTTGTTCCTGGGAGTTCATATTCGAGCTATAACTCAGCTCAAGAAACTCTGAGGTCATTACTGCATAGTGTGGTTAAGGTTCCACATATAAAAAATATGGTTGTAGAGCTAAATGGTGTATTGGTCACTCATGCTTTAATAACTGTTCGCGAACTACTTCTCCGATGTGTAAAACATTACTCATGGTATGCAATGAGAGCAATCTACATAGCAAAGGGCAGTCCGTTGCTTCCTCCAGCTTTCGCATCCATGTTTGATGATTTTTCATCGTCCTCTCTTGATGCCTTTTTTGATCCTTCTCGTGGCTTGGTGAACGTCCCTGGTTTGACAGTGGGCACCTTCAAACTCCTCAGCAAACTTATTGACAACAAAGGATTGTCAGGGACAAGACGTTACTTTGGTGATCTTGGGAAAACTCTAAGAACAGCAGGATCCAATGTCGTCTTTGTTGCTCTCACTGAAATCTCCGATTCTGTTCTGAGGGGTGCAGAAATGAAAGGTGTTGATGGACTGGTTAGCGGTTTCCACCATGGAATCCTTAAACTGGCAATGGAACCGTCTGTGATAGGAACAGCTTTGATGGAAGGTGGCCCTGATAGAACAATTAAGCTTGATCGTAACCCCGGTATTGATGAGTTATACATCGAAGGATACCTTCAAGCTATGTTGGATACAATGTATAGGCAAGAGTATCTCAGAGTCAAAGTCATTGACGATCAGGTTTTCCTGAAAAACCTCCCACCAAGCAATTCTCTCATAGATGAGATGATAGACCGTGTTAAAGATTTTCTCGAAAGCAGGGGTTTACTCAAAGGAGATCCCTCAAGCTCTCGTCCTCGTCGCCGTCTCCATGGAGACAAGGAATGGAAGATCGGACCAACGGTGTTGACATTGTGTGAACATCTATTCGTCAGTTTTGCAATTCGCATATTGAAACAGCACGCCACTAAGGCCATTACAAGTCTAAGGCCAAAGAAAGAAGAAGCAGAGGCCGAGACAAGTGACAGTGGTTCCAATACTGCAATGGTACCAGTAGTTAGTGACAATAAGAAGAAGAAAATGAAGTTCATGTGGAAAGCTGGTATTGGGAACTTCGTTGCTTCAGGTATCGTAGCTTACATCGACGGACGTTTGTGCCGACAAATTCCTAATCCAATTGCTCGCCGGATTGTGAGTGGGTTTTTGTTAAGTTTTCTCGATAAAAGCAGTGAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHR-BD VPS13_C

Protein Analysis

3072

Amino Acids

343.75

Weight (kDa)

6.6

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_VAB PF25036 2050 - 2467 1.6e-26 Vacuolar-sorting associated protein 13, adaptor binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1289, 2340, 4014, 4602
AarI CACCTGC 1 cut(s) 6145
AasI GACNNNNNNGTC 3 cut(s) 725, 4040, 6640
Acc36I ACCTGC 1 cut(s) 6145
Acc65I GGTACC 4 cut(s) 3612, 4364, 4789, 9028
AccB1I GGYRCC 6 cut(s) 3612, 4364, 4789, 7726, 8336, 9028
AccB7I CCANNNNNTGG 1 cut(s) 4449
AccBSI CCGCTC 1 cut(s) 6261
AccI GTMKAC 4 cut(s) 195, 1553, 2061, 2892
AccII CGCG 4 cut(s) 63, 255, 777, 8151
AccIII TCCGGA 1 cut(s) 4716
AclI AACGTT 1 cut(s) 5457
AcoI YGGCCR 2 cut(s) 1231, 2330
AcvI CACGTG 1 cut(s) 1498
AflII CTTAAG 2 cut(s) 2590, 2734
AflIII ACRYGT 3 cut(s) 1439, 1495, 4039
AgeI ACCGGT 1 cut(s) 4073
AhdI GACNNNNNGTC 2 cut(s) 2353, 4773
AjiI CACGTC 1 cut(s) 2350
AjuI GAANNNNNNNTTGG 4 cut(s) 393, 425, 5470, 5502
AleI CACNNNNGTG 2 cut(s) 1695, 6957
AloI GAACNNNNNNTCC 2 cut(s) 8145, 8177
Alw21I GWGCWC 4 cut(s) 1131, 4399, 4997, 7080
Alw44I GTGCAC 1 cut(s) 7076
AlwNI CAGNNNCTG 4 cut(s) 1579, 2416, 5411, 6203
Ama87I CYCGRG 3 cut(s) 301, 5100, 7455
Aor13HI TCCGGA 1 cut(s) 4716
ApaLI GTGCAC 1 cut(s) 7076
AseI ATTAAT 1 cut(s) 498
AsiGI ACCGGT 1 cut(s) 4073
Asp700I GAANNNNTTC 5 cut(s) 3999, 5052, 6135, 6906, 8157
Asp718I GGTACC 4 cut(s) 3612, 4364, 4789, 9028
AspLEI GCGC 4 cut(s) 2157, 4106, 4208, 7025
AspS9I GGNCC 9 cut(s) 1726, 2225, 2375, 5231, 5534, 7187, 7294, 8591, 8876
AsuC2I CCSGG 2 cut(s) 519, 8625
AsuII TTCGAA 6 cut(s) 246, 3558, 5820, 6043, 6933, 7520
AvaI CYCGRG 3 cut(s) 301, 5100, 7455
AvaII GGWCC 6 cut(s) 1726, 5231, 5534, 7187, 7294, 8876
AxyI CCTNAGG 2 cut(s) 2492, 4499
BaeGI GKGCMC 4 cut(s) 1156, 7080, 7729, 8339
BamHI GGATCC 1 cut(s) 8432
BanI GGYRCC 6 cut(s) 3612, 4364, 4789, 7726, 8336, 9028
BanII GRGCYC 4 cut(s) 1131, 2047, 4399, 4497
BarI GAAGNNNNNNTAC 2 cut(s) 3844, 3876
BauI CACGAG 1 cut(s) 8301
BbrPI CACGTG 1 cut(s) 1498
BbsI GAAGAC 5 cut(s) 1605, 2934, 4038, 5618, 7637
Bbv12I GWGCWC 4 cut(s) 1131, 4399, 4997, 7080
BbvCI CCTCAGC 2 cut(s) 560, 8351
BceAI ACGGC 5 cut(s) 1246, 5295, 6080, 7401, 8829
BcgI CGANNNNNNTGC 6 cut(s) 1127, 1161, 5754, 5788, 7840, 7874
BciVI GTATCC 2 cut(s) 8645, 8665
BclI TGATCA 5 cut(s) 1771, 6205, 6550, 6589, 7963
BcnI CCSGG 2 cut(s) 519, 8625
BfoI RGCGCY 1 cut(s) 4107
BfrI CTTAAG 2 cut(s) 2590, 2734
BfuAI ACCTGC 1 cut(s) 6145
BfuI GTATCC 2 cut(s) 8645, 8665
BglI GCCNNNNNGGC 1 cut(s) 8951
BglII AGATCT 1 cut(s) 6656
BmcAI AGTACT 3 cut(s) 1489, 1861, 7182
Bme18I GGWCC 6 cut(s) 1726, 5231, 5534, 7187, 7294, 8876
BmeRI GACNNNNNGTC 2 cut(s) 2353, 4773
BmeT110I CYCGRG 3 cut(s) 301, 5100, 7455
BmgBI CACGTC 1 cut(s) 2350
BmgT120I GGNCC 9 cut(s) 1726, 2225, 2375, 5231, 5534, 7187, 7294, 8591, 8876
BmrI ACTGGG 4 cut(s) 1656, 3640, 3665, 4627
BmuI ACTGGG 4 cut(s) 1656, 3640, 3665, 4627
BoxI GACNNNNGTC 3 cut(s) 3360, 4886, 7457
BpiI GAAGAC 5 cut(s) 1605, 2934, 4038, 5618, 7637
BplI GAGNNNNNCTC 4 cut(s) 6300, 6332, 7909, 7941
Bpu10I CCTNAGC 2 cut(s) 560, 8351
Bpu14I TTCGAA 6 cut(s) 246, 3558, 5820, 6043, 6933, 7520
BpuMI CCSGG 2 cut(s) 519, 8625
Bsa29I ATCGAT 2 cut(s) 5582, 6056
BsaAI YACGTR 5 cut(s) 982, 1498, 3492, 4040, 7149
BsaBI GATNNNNATC 2 cut(s) 3293, 6411
BsaI GGTCTC 4 cut(s) 2557, 4616, 5612, 5968
BsaJI CCNNGG 9 cut(s) 372, 1260, 2719, 7301, 8009, 8320, 8530, 8623, 8851
BsaWI WCCGGW 4 cut(s) 817, 2662, 4073, 4716
BsaXI ACNNNNNCTCC 6 cut(s) 22, 52, 4275, 4305, 8145, 8175
Bse118I RCCGGY 1 cut(s) 4073
Bse21I CCTNAGG 2 cut(s) 2492, 4499
Bse3DI GCAATG 4 cut(s) 6113, 8199, 8556, 9030
Bse8I GATNNNNATC 2 cut(s) 3293, 6411
BseAI TCCGGA 1 cut(s) 4716
BseCI ATCGAT 2 cut(s) 5582, 6056
BseDI CCNNGG 9 cut(s) 372, 1260, 2719, 7301, 8009, 8320, 8530, 8623, 8851
BseJI GATNNNNATC 2 cut(s) 3293, 6411
BseMI GCAATG 4 cut(s) 6113, 8199, 8556, 9030
BseRI GAGGAG 6 cut(s) 577, 3873, 4212, 7601, 7837, 8339
BseSI GKGCMC 4 cut(s) 1156, 7080, 7729, 8339
BseYI CCCAGC 4 cut(s) 1013, 1729, 2416, 2638
BsgI GTGCAG 4 cut(s) 3522, 6173, 6511, 8508
Bsh1236I CGCG 4 cut(s) 63, 255, 777, 8151
BshNI GGYRCC 6 cut(s) 3612, 4364, 4789, 7726, 8336, 9028
BshTI ACCGGT 1 cut(s) 4073
BshVI ATCGAT 2 cut(s) 5582, 6056
BsiHKAI GWGCWC 4 cut(s) 1131, 4399, 4997, 7080
BsiHKCI CYCGRG 3 cut(s) 301, 5100, 7455
BsiWI CGTACG 1 cut(s) 990
BslFI GGGAC 9 cut(s) 298, 2772, 3194, 3827, 4925, 5577, 6584, 8303, 8398
BsmBI CGTCTC 3 cut(s) 4543, 5661, 8852
BsmFI GGGAC 9 cut(s) 298, 2772, 3194, 3827, 4925, 5577, 6584, 8303, 8398
BsmI GAATGC 6 cut(s) 2083, 4819, 5374, 6039, 7132, 7505
Bso31I GGTCTC 4 cut(s) 2557, 4616, 5612, 5968
BsoBI CYCGRG 3 cut(s) 301, 5100, 7455
Bsp119I TTCGAA 6 cut(s) 246, 3558, 5820, 6043, 6933, 7520
Bsp1286I GDGCHC 9 cut(s) 1131, 1156, 2047, 4399, 4497, 4997, 7080, 7729, 8339
Bsp13I TCCGGA 1 cut(s) 4716
Bsp1407I TGTACA 2 cut(s) 502, 4960
Bsp19I CCATGG 3 cut(s) 7301, 8530, 8851
Bsp68I TCGCGA 2 cut(s) 63, 8151
BspDI ATCGAT 2 cut(s) 5582, 6056
BspEI TCCGGA 1 cut(s) 4716
BspFNI CGCG 4 cut(s) 63, 255, 777, 8151
BspHI TCATGA 2 cut(s) 1641, 4753
BspMAI CTGCAG 4 cut(s) 619, 2890, 5150, 5416
BspMI ACCTGC 1 cut(s) 6145
BspQI GCTCTTC 1 cut(s) 5862
BspT104I TTCGAA 6 cut(s) 246, 3558, 5820, 6043, 6933, 7520
BspT107I GGYRCC 6 cut(s) 3612, 4364, 4789, 7726, 8336, 9028
BspTI CTTAAG 2 cut(s) 2590, 2734
BspTNI GGTCTC 4 cut(s) 2557, 4616, 5612, 5968
BsrBI CCGCTC 1 cut(s) 6261
BsrDI GCAATG 4 cut(s) 6113, 8199, 8556, 9030
BsrFI RCCGGY 1 cut(s) 4073
BsrGI TGTACA 2 cut(s) 502, 4960
BssAI RCCGGY 1 cut(s) 4073
BssECI CCNNGG 9 cut(s) 372, 1260, 2719, 7301, 8009, 8320, 8530, 8623, 8851
BssNAI GTATAC 1 cut(s) 1554
BssSI CACGAG 1 cut(s) 8301
BssT1I CCWWGG 4 cut(s) 372, 7301, 8530, 8851
Bst1107I GTATAC 1 cut(s) 1554
Bst2BI CACGAG 1 cut(s) 8301
Bst6I CTCTTC 8 cut(s) 3035, 3050, 4664, 4843, 5657, 5862, 6798, 7881
BstAFI CTTAAG 2 cut(s) 2590, 2734
BstAPI GCANNNNNTGC 3 cut(s) 5732, 6110, 7733
BstAUI TGTACA 2 cut(s) 502, 4960
BstBAI YACGTR 5 cut(s) 982, 1498, 3492, 4040, 7149
BstBI TTCGAA 6 cut(s) 246, 3558, 5820, 6043, 6933, 7520
BstDSI CCRYGG 4 cut(s) 2719, 7301, 8530, 8851
BstEII GGTNACC 2 cut(s) 1931, 4076
BstENI CCTNNNNNAGG 1 cut(s) 7316
BstFNI CGCG 4 cut(s) 63, 255, 777, 8151
BstH2I RGCGCY 1 cut(s) 4107
BstHHI GCGC 4 cut(s) 2157, 4106, 4208, 7025
BstNSI RCATGY 7 cut(s) 1443, 1696, 2961, 3123, 4214, 6108, 6248
BstPAI GACNNNNGTC 3 cut(s) 3360, 4886, 7457
BstPI GGTNACC 2 cut(s) 1931, 4076
BstSLI GKGCMC 4 cut(s) 1156, 7080, 7729, 8339
BstSNI TACGTA 1 cut(s) 982
BstUI CGCG 4 cut(s) 63, 255, 777, 8151
BstV2I GAAGAC 5 cut(s) 1605, 2934, 4038, 5618, 7637
BstX2I RGATCY 7 cut(s) 1657, 2145, 4800, 5104, 6656, 8432, 8818
BstYI RGATCY 7 cut(s) 1657, 2145, 4800, 5104, 6656, 8432, 8818
BstZ17I GTATAC 1 cut(s) 1554
Bsu15I ATCGAT 2 cut(s) 5582, 6056
Bsu36I CCTNAGG 2 cut(s) 2492, 4499
BsuI GTATCC 2 cut(s) 8645, 8665
BsuTUI ATCGAT 2 cut(s) 5582, 6056
BtgI CCRYGG 4 cut(s) 2719, 7301, 8530, 8851
BtgZI GCGATG 2 cut(s) 78, 3924
BtrI CACGTC 1 cut(s) 2350
BtsI GCAGTG 4 cut(s) 1774, 2790, 6714, 9214
BtuMI TCGCGA 2 cut(s) 63, 8151
BveI ACCTGC 1 cut(s) 6145
CaiI CAGNNNCTG 4 cut(s) 1579, 2416, 5411, 6203
CciI TCATGA 2 cut(s) 1641, 4753
CfoI GCGC 4 cut(s) 2157, 4106, 4208, 7025
Cfr10I RCCGGY 1 cut(s) 4073
Cfr13I GGNCC 9 cut(s) 1726, 2225, 2375, 5231, 5534, 7187, 7294, 8591, 8876
ClaI ATCGAT 2 cut(s) 5582, 6056
CseI GACGC 6 cut(s) 370, 3191, 3415, 3562, 7781, 7816
CspAI ACCGGT 1 cut(s) 4073
DraI TTTAAA 1 cut(s) 4744
DrdI GACNNNNNNGTC 3 cut(s) 725, 4040, 6640
DriI GACNNNNNGTC 2 cut(s) 2353, 4773
DseDI GACNNNNNNGTC 3 cut(s) 725, 4040, 6640
EaeI YGGCCR 2 cut(s) 1231, 2330
Eam1104I CTCTTC 8 cut(s) 3035, 3050, 4664, 4843, 5657, 5862, 6798, 7881
Eam1105I GACNNNNNGTC 2 cut(s) 2353, 4773
EarI CTCTTC 8 cut(s) 3035, 3050, 4664, 4843, 5657, 5862, 6798, 7881
EciI GGCGGA 2 cut(s) 3756, 6444
Ecl136II GAGCTC 2 cut(s) 1129, 4397
Eco105I TACGTA 1 cut(s) 982
Eco130I CCWWGG 4 cut(s) 372, 7301, 8530, 8851
Eco147I AGGCCT 3 cut(s) 2401, 3461, 4228
Eco24I GRGCYC 4 cut(s) 1131, 2047, 4399, 4497
Eco31I GGTCTC 4 cut(s) 2557, 4616, 5612, 5968
Eco32I GATATC 1 cut(s) 3106
Eco47I GGWCC 6 cut(s) 1726, 5231, 5534, 7187, 7294, 8876
Eco53kI GAGCTC 2 cut(s) 1129, 4397
Eco72I CACGTG 1 cut(s) 1498
Eco81I CCTNAGG 2 cut(s) 2492, 4499
Eco88I CYCGRG 3 cut(s) 301, 5100, 7455
Eco91I GGTNACC 2 cut(s) 1931, 4076
EcoICRI GAGCTC 2 cut(s) 1129, 4397
EcoNI CCTNNNNNAGG 1 cut(s) 7316
EcoO109I RGGNCCY 1 cut(s) 2375
EcoO65I GGTNACC 2 cut(s) 1931, 4076
EcoRI GAATTC 4 cut(s) 1186, 2473, 3532, 4435
EcoRV GATATC 1 cut(s) 3106
EcoT14I CCWWGG 4 cut(s) 372, 7301, 8530, 8851
EcoT22I ATGCAT 3 cut(s) 2963, 6076, 7213
EcoT38I GRGCYC 4 cut(s) 1131, 2047, 4399, 4497
ErhI CCWWGG 4 cut(s) 372, 7301, 8530, 8851
Esp3I CGTCTC 3 cut(s) 4543, 5661, 8852
FaqI GGGAC 9 cut(s) 298, 2772, 3194, 3827, 4925, 5577, 6584, 8303, 8398
FauI CCCGC 2 cut(s) 2099, 5037
FauNDI CATATG 2 cut(s) 1918, 4317
FbaI TGATCA 5 cut(s) 1771, 6205, 6550, 6589, 7963
FblI GTMKAC 4 cut(s) 195, 1553, 2061, 2892
FriOI GRGCYC 4 cut(s) 1131, 2047, 4399, 4497
GlaI GCGC 4 cut(s) 2156, 4105, 4207, 7024
GsaI CCCAGC 4 cut(s) 1017, 1733, 2420, 2642
HaeII RGCGCY 1 cut(s) 4107
HgaI GACGC 6 cut(s) 370, 3191, 3415, 3562, 7781, 7816
HhaI GCGC 4 cut(s) 2157, 4106, 4208, 7025
Hin6I GCGC 4 cut(s) 2155, 4104, 4206, 7023
HinP1I GCGC 4 cut(s) 2155, 4104, 4206, 7023
HincII GTYRAC 6 cut(s) 196, 703, 1341, 1756, 4663, 8889
HindII GTYRAC 6 cut(s) 196, 703, 1341, 1756, 4663, 8889
HindIII AAGCTT 3 cut(s) 5397, 7238, 8609
HpaI GTTAAC 1 cut(s) 1756
Hpy99I CGWCG 5 cut(s) 30, 3366, 3431, 8844, 9128
HspAI GCGC 4 cut(s) 2155, 4104, 4206, 7023
Kpn2I TCCGGA 1 cut(s) 4716
KpnI GGTACC 4 cut(s) 3616, 4368, 4793, 9032
Ksp22I TGATCA 5 cut(s) 1771, 6205, 6550, 6589, 7963
KspAI GTTAAC 1 cut(s) 1756
LguI GCTCTTC 1 cut(s) 5862
LmnI GCTCC 7 cut(s) 235, 1134, 2413, 2917, 3683, 4283, 5202
MbiI CCGCTC 1 cut(s) 6261
MfeI CAATTG 9 cut(s) 16, 1837, 2616, 4863, 5266, 5706, 6270, 7026, 9156
MflI RGATCY 7 cut(s) 1657, 2145, 4800, 5104, 6656, 8432, 8818
MhlI GDGCHC 9 cut(s) 1131, 1156, 2047, 4399, 4497, 4997, 7080, 7729, 8339
Mph1103I ATGCAT 3 cut(s) 2963, 6076, 7213
MroI TCCGGA 1 cut(s) 4716
MroXI GAANNNNTTC 5 cut(s) 3999, 5052, 6135, 6906, 8157
MslI CAYNNNNRTG 6 cut(s) 1514, 1695, 1767, 5009, 6218, 6957
MspA1I CMGCKG 2 cut(s) 4682, 6488
MspCI CTTAAG 2 cut(s) 2590, 2734
MunI CAATTG 9 cut(s) 16, 1837, 2616, 4863, 5266, 5706, 6270, 7026, 9156
Mva1269I GAATGC 6 cut(s) 2083, 4819, 5374, 6039, 7132, 7505
MvnI CGCG 4 cut(s) 63, 255, 777, 8151
NciI CCSGG 2 cut(s) 519, 8625
NcoI CCATGG 3 cut(s) 7301, 8530, 8851
NdeI CATATG 2 cut(s) 1918, 4317
NmeAIII GCCGAG 2 cut(s) 3713, 9019
NruI TCGCGA 2 cut(s) 63, 8151
NsiI ATGCAT 3 cut(s) 2963, 6076, 7213
NspI RCATGY 7 cut(s) 1443, 1696, 2961, 3123, 4214, 6108, 6248
NspV TTCGAA 6 cut(s) 246, 3558, 5820, 6043, 6933, 7520
OliI CACNNNNGTG 2 cut(s) 1695, 6957
PaeI GCATGC 2 cut(s) 2961, 3123
PaeR7I CTCGAG 2 cut(s) 301, 7455
PagI TCATGA 2 cut(s) 1641, 4753
PaqCI CACCTGC 1 cut(s) 6145
PceI AGGCCT 3 cut(s) 2401, 3461, 4228
PciI ACATGT 1 cut(s) 1439
PciSI GCTCTTC 1 cut(s) 5862
PcsI WCGNNNNNNNCGW 4 cut(s) 34, 124, 5647, 9136
PctI GAATGC 6 cut(s) 2083, 4819, 5374, 6039, 7132, 7505
PdmI GAANNNNTTC 5 cut(s) 3999, 5052, 6135, 6906, 8157
Pfl23II CGTACG 1 cut(s) 990
PflMI CCANNNNNTGG 1 cut(s) 4449
PfoI TCCNGGA 4 cut(s) 1022, 2949, 3702, 5530
PinAI ACCGGT 1 cut(s) 4073
PmaCI CACGTG 1 cut(s) 1498
PmlI CACGTG 1 cut(s) 1498
Ppu21I YACGTR 5 cut(s) 982, 1498, 3492, 4040, 7149
PscI ACATGT 1 cut(s) 1439
PshAI GACNNNNGTC 3 cut(s) 3360, 4886, 7457
PshBI ATTAAT 1 cut(s) 498
PsiI TTATAA 4 cut(s) 1289, 2340, 4014, 4602
Psp124BI GAGCTC 2 cut(s) 1131, 4399
Psp1406I AACGTT 1 cut(s) 5457
PspCI CACGTG 1 cut(s) 1498
PspEI GGTNACC 2 cut(s) 1931, 4076
PspFI CCCAGC 4 cut(s) 1013, 1729, 2416, 2638
PspLI CGTACG 1 cut(s) 990
PspPI GGNCC 9 cut(s) 1726, 2225, 2375, 5231, 5534, 7187, 7294, 8591, 8876
PspXI VCTCGAGB 1 cut(s) 7455
PstI CTGCAG 4 cut(s) 619, 2890, 5150, 5416
PstNI CAGNNNCTG 4 cut(s) 1579, 2416, 5411, 6203
PsuI RGATCY 7 cut(s) 1657, 2145, 4800, 5104, 6656, 8432, 8818
PvuII CAGCTG 2 cut(s) 4682, 6488
RruI TCGCGA 2 cut(s) 63, 8151
RseI CAYNNNNRTG 6 cut(s) 1514, 1695, 1767, 5009, 6218, 6957
SacI GAGCTC 2 cut(s) 1131, 4399
SalI GTCGAC 1 cut(s) 194
SapI GCTCTTC 1 cut(s) 5862
Sau96I GGNCC 9 cut(s) 1726, 2225, 2375, 5231, 5534, 7187, 7294, 8591, 8876
ScaI AGTACT 3 cut(s) 1489, 1861, 7182
SduI GDGCHC 9 cut(s) 1131, 1156, 2047, 4399, 4497, 4997, 7080, 7729, 8339
Sfr274I CTCGAG 2 cut(s) 301, 7455
SfuI TTCGAA 6 cut(s) 246, 3558, 5820, 6043, 6933, 7520
SinI GGWCC 6 cut(s) 1726, 5231, 5534, 7187, 7294, 8876
SlaI CTCGAG 2 cut(s) 301, 7455
SmiMI CAYNNNNRTG 6 cut(s) 1514, 1695, 1767, 5009, 6218, 6957
SnaBI TACGTA 1 cut(s) 982
SphI GCATGC 2 cut(s) 2961, 3123
SseBI AGGCCT 3 cut(s) 2401, 3461, 4228
SspI AATATT 4 cut(s) 1117, 1997, 3823, 7955
SstI GAGCTC 2 cut(s) 1131, 4399
StuI AGGCCT 3 cut(s) 2401, 3461, 4228
StyI CCWWGG 4 cut(s) 372, 7301, 8530, 8851
TaqII GACCGA 1 cut(s) 3354
TatI WGTACW 8 cut(s) 502, 1487, 1859, 4960, 5016, 6085, 7180, 7931
Van91I CCANNNNNTGG 1 cut(s) 4449
Vha464I CTTAAG 2 cut(s) 2590, 2734
VneI GTGCAC 1 cut(s) 7076
VpaK11BI GGWCC 6 cut(s) 1726, 5231, 5534, 7187, 7294, 8876
VspI ATTAAT 1 cut(s) 498
XagI CCTNNNNNAGG 1 cut(s) 7316
XbaI TCTAGA 1 cut(s) 1717
XceI RCATGY 7 cut(s) 1443, 1696, 2961, 3123, 4214, 6108, 6248
XcmI CCANNNNNNNNNTGG 4 cut(s) 4097, 5426, 6574, 9022
XhoI CTCGAG 2 cut(s) 301, 7455
XmiI GTMKAC 4 cut(s) 195, 1553, 2061, 2892
XmnI GAANNNNTTC 5 cut(s) 3999, 5052, 6135, 6906, 8157
ZrmI AGTACT 3 cut(s) 1489, 1861, 7182
Zsp2I ATGCAT 3 cut(s) 2963, 6076, 7213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.