Rroxscaffold_2G00139720

regulation of parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004207
Physical Location & Seq
Forward (+)
78067461 .. 78087917
20457 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139720.1

Sequence Viewer

Length: 9714 bp
ATGTTTCCAAGCGGCACAATCCGGCGGAGGCTGGCCGCTCTGCTGCGGCCCTGGCTTCGGGAAGAGCCGGAACTCGAGCTCAGTTTGGGGCTGATCAGTTCGCACGCGGTGGCTAAGAACCTCGGGTTCGATACCGCGGCGCTCAATCGGCTCGGCGACGATTCGGACGGGTTTTTGTATAAGGAGGTCAGTGTCCAACAGTTGAGCGTTCGGTTCTCGAACTGGCTCGCTCCTGCATTCTCAATTGAGTTTCATGGCGTCAAAGTGGTGCTCTCGACTCGGGAGTTGAAGAAGAAGAAGAAGAAGAAGAAGCTTTCTGAGGATACATTTGCAGAGGATATGAAGAAGAAGAAGCTTTCTGAGATTGATCCTCAGGGGAGTGCTGTGCATGCTTTTCTGGAAAAGCTTTTGGCTACTTCTACGACTCCGAAAAATAAGTTCAAAACATCTCTTTTGAATCTGTTACTCACACACTGTCAGCTTCAAGTGCATGATATTAGTGTCCAAGTCCAGGTTCCGATCTTAAATGATTCCTTTGTCTGTTCACTGGATATAGAAGATATAAATGTAGATCCTCAGTATATTGATCGTGGGTGTCTTCTTCGAGGCCTTTTTGGTACATTCTTTGTACCTATGAAGGAGAGCTCTTTCGCTATAGTAGGCAGTGGTTGTGAGGTTGGTTTTAAGAGGGCAGGTCAACTCAAACGTGTCTTGTTATTGACTGAGTTGTGCAGTTGCATTAAGTTGAATGATTTTCAGCTTGTAGACATCAATCTTCGAGTCCCTGAAATACATTTTTCGTTTTCTCCGGATGATGTTTCTTTCTATTTGGCATTCACTACAGCATCATCCCAAGATTCTCATCATGCTAGAGATGGCAGACAACTGTGGAAGCTAGCTGCGAGTAGAATTGACAATATGACTTCAGCTCATAGGTGGTCGTTGCAAAAGTCAGCTGTCGTTGTTTGTTTCTGGTTACGTTATGTCAATGCTTACGAGCATTTACTGTTGCTAATTGGATATTATGACGACCACTTATTAAAAAGATCAGCTATTAGAATCTATGAGGACAAGATGCTTTTGAGTTCTGTTGAAAACCAATGGAAGGTGATTTCTGATATTGAGAAAGAGCTGCCAGCTGAAGCTATTGCACAGGCATGGAGAGTAGCCCGTAACAGAGCAGCATCAAATGTTCAATGTCCCGAATATAGTTCCAAAAAACCATTTGTGACTACAATTTACAACTTCCTTCTCATAATCCTTTCATTACTAGCTTGTGCCTGGAGATTTTTTTGCAAGATAGTCCTTTTTATTATGCACCCCTTTGTTTTTAGGAAAATATTAGTCAATGAGCCAAAGAGTGCAGATCTGGATATTGTTTCTGAAGGTCCGTGTACGCAGTTTTGCTTCAGTTTGATGCTTGGAAAAGTTCAAATCACTATCTCTCATAGAAATGAAATTCAACTCTTAGTAAATGAAAAGTTGAAATCACATCTTGGGATCACTTACTCAGATTCACTTTCATTTCGTCTTTCAGTTGACGCACTGTTATTAAAATATGTAGAGGACATGTGTGAAGAGTCTTTACTGATATCCTGTGGGCAATTAAAGGTCAGATCCTCATCTCTTATGGAAGCTCCTGTTAAAGAAAGCAGTTCAAAATTATCCTTTAGTTCCATGGAAGCACATTGGAAGGAGAGCAATGATAAGTGGAACAATATTCTGTGGGGTGAGCCAGCAGAAATATTTCCACTTTTAGAAACTTCTGAAACTGGTTGTGCTGATCATGTGGAAGGTTCTTGTGTCTCATTTCTGAAAGACATGTGGTTGGATTGGAGAAGTGAGTGCGATAAATTTGGAAAAAGCGAAATTCAGTACTCTGAAACTCCATTCCTTCTTTGTGAATTTAAAAACTTCTTGATATATCCAGACCTCAAAACATTGGATTCTGGGTTTTGGAAGTTCTATTTTGTTCTGGGAAAGTTGAATCTTGTGTTGGGATATTCATCAATAGTATCATTGTCTCTGCTATTTAGGCAGATACACCATACTCAATGCTGGGGAGAGGCCAATGGTCAGTCACCTGGTCTTTCACATTCTCCAAGAACCAGTGAGAGTAAACCAGAAATCAGTCTGGCTAGCAAATACAAGTGCTATGCTAATAGATTAGAAATGGCATTGCTTAAAATGCTTCCAAAAAAACATGTTCAACTAGGGGTATTTATTGCTGGTCCTCACATCCTTATGTCATTAGGAAAGAATTTTGATGGTGGCAACAAAGACAAAAATCATGTATATATTCAGGACGATTTTCACCTTGCATTTGATGTGCACAATATTGAAGCTGCTGTTTGGCCAACCTCAAAGTTTGATCTTGCATCATTCGTGGGGCCCTCAGCATCTGATGATGTGGAACCAGAGTGCCTCAGGATGGACCAGCCTCTGGTGATTGATATCTCTAACTCTAATAATGGGAAGTATCAAGCTCAGGGTGGGATCTCTCTTGGTTCCTACATACGAGTTGATGGTTTAGATGTCTGCCTGGTCGCCGCAGCAGGAAAACGACAAAGCCAGATTTTGGTTTCTAAGCCAATGACTTTGCAGTTATTATCCTCCAGGGAGTATGTTCATTCATTTAGCACAAATGTTATTGCTTTCTCTGCAGCTTTGTTTGGGAAAACTGAAGGATTCACTATTTTATCGTACATGGATGAGTTTGATGTTCTCTTTCAGGTGCTTGCAAATTTATCTTCTGCAGTATCATATTCGTTTAGACATTTTGCATCTGTCAGTGACTTGCCTTTACAATTTGGAAAGCAACAATATTCAAATGCTGAATCTGAGAACGAGCAGACCACAGTGCATGAACCTCCCTTGAATTATAGCAGTGTCCTATTTTCAACTAATGGGACCTTCAAAATCAAGTCAATGAATATAATTGTGCACAAGTCTAGGATGCGTGATAATGTGGGGAGCTACCTAGTAACCTCTGATGCTTCAAGTAGCAAACAGTTGTCTGAGCATGACTTGCCTGATTATGGAATTTGGATTTCAGTTCATCAAACAAGTCTTGATATGTCTTACAAAGAAGGAAAAGTGATACTTCTTTCTAATTTATCAGAAATTCAGTCTTCAAGTTTTAGATATAAGAATCGGAGAGGCAAAAGTACTGATCAGTCTGTACTTAGTGATCTGCTACTGCAATCTCTTGATTGCATATATCAACTATCACTTTCTAGTTGTGTACTTAGTTTGTCACTGTTCCTATCTCAGAACTGCTCATCTATTGGCACTGTTAGCAACACACTCAATTCTAATTCTGTGAGTGAAACAGAGCACACGGAAAATTTCCCATTCACTAATTCTGAAAGCTCAGGTCATCAGAACTATAGCTTTATTCAAGGGTCAGAATTGGCTTCAAATATTCGACCACCGGGCTCAAGCCATTGGTTACTTGTAAATCTTTCACTGGGTAAGATTTATGTGGGAAGGTGCTCAGCAAAGAATGTTATGAATGAGGCGCATCAATTAAATAAGTTTTTGTCATCAGTTTCTGTTGGTGGTGAATTTCAGAGAATCAGTTGTGAAATTCAGGGTGGTTTCCTTTTCCTTGAAACAACAGCTTCGGCTACCTTATTGCGATGTTTTTCTTCATATCTTGATTGCTTCACAAATATTTTATCTGGCTCACAATCATCCGATAAGCACGGTGAAGAAGATAAGCCCATTGAAGAAGCTGATACTGCTGTAAATATGACTAGACCAGATAGTCATTCTGATAAGGACTGTATACAAGGGACTCCTTGTACATCTCCACAGGTCAAAGGGGGACAGATGGAAGCTTTTAGCTTAAATATATCTCGTTTTTCTTGCGCTCTTGTGATTCAGGAAGAGAATGGTGCTGGACAGGAGCTCGTGCTTGAGATTGATGCCCATTTGAACGTTGAGTTGGAAAACATGCGAAGAAAATTTATATTTAAGCTTTCTCGCATATCAATTCTTTCTCAGGTCCTTCAAGAAATTTTGGAAAATCAAACCCAGAGTTCTCAAGTTTCTTCAGTCACATCAAATATTTTTCAATCTCATGCTGCATCAGGAGCGTCTACAGGTTCTCAGCACATGGATGAGATTCATCCTGTTAATAATGCAAGTAGTTCAAGAGGTCCTGGATCACAGGAAGAGCGGTCTGAACATAGTTCTCTGCATGAGGTATTTCGTCTAAGTCATCACAAATATATCCTAAAGGGTCAAGAATTACCCTCCAGTGAGTGCGAGTCAAGACAGGAAGGAGGAACTGCCTTCATATCAGTTGAGAAGCCTTTAAATGAAGTTTGGGTTGGAAGTGGAACTATTTCATGTTTCAATATTACAATATCTCTATCTCAAATACAGATGCTTTTGTCTATGATTTCATCCTTTTCTGGGGTATTTGGGGAGGAGGTGATCAGTGAACCGGATAGAAGGCACTGGTCGAGTGACGAGGAATTAAAAAACAGTTTGGAGACAATGGTTCCTAATGGTGCAATAGTGGCAATTCAGGATGTCCATCAACATATGTATTTTACAGTTGAGGGTAATGAGGATAAGTACAGCTTAGCTGGTGCAGTTCATTATTCTCTTGTGGGAGAAAGCGCTCTTTTCATGGTCAAGTATAACAATCAAAAGGGATGGAATTCATCAGTTTTGTGGTTCTCCTTGATATCGTTGCATGCCAAGAATGCTTCTGGGGAGCCATTGCGCTTGAACTACCGTCGAGGATCTGATTTTGTTGACGTCTCTAGTGCGAATGATAATGCAGGGGCACTTTGGACAGCGGTTTCTTGTGAGCCTGAAAGTTATGAGGGCGACATTGACTGGGAGCCCTACAATCAATTGATTAAAAGGACCTTTTACCTTGTGAACAAAAAGAATGACTCTGCTGTTGCTATTGTAGATGGGATTCCAGAGTTTGTTAGAAAGCCTGGAAATCCAATCAAGTTGAAAGTGTTCCACAATGTTCCTATAGCTCATGACATAAAGATGGATAACTACCCTGGAGAGGCATCTGTTGCTCGTCCACAACAGAGTGATGAAGGGATAACTTCTGGGAACGGTGGAAAGCTTCCTTGCATTGATGTTACATTTGACACAATTTCCTTGACCATTATCCATGAACTTGTAGATACAAAAGACATGCCTCTCCTTCGTTGTCGCATTGGCGGAACTGGACTGTCCAAACATGAACTTGAAGAGTCAAAAGACATGGCTCTCCTTGGTGGTTGCAGTGACAGAACCGAACGGTCCAAACTTACTGTTCAAATTCTCCCTTCTAAAGTTAGGATTATAAGCTCCTTAACAACAGTGGTATATTACTTTGATGCACAAAGAAACAAATGGAGAGAACTTATCCACCCAGTTGAAACATGCCTTTTCTATCGTTCAAGCTTCCAAATTCAGAGTTCAGAAGCTGTTTCGCATGGAGTACCTGTTCATATCCATTGCAGAACTAAAGAGTTGAATATATCCCTATCCGAGCTTTCATTGGACATACTTCTCTTTACAGTTGGGAAACTAAATTTAGCTGGTCCTTTCTCAGTGAGAAGTTCTAAGATTTGGGCCAATTGTTGCAAGGTGGAGAACCAGTCAGGATTAAACCTTCTTTGTCAGTATGATGAGCAAAGTGTAAAAGTTTCCAGAAGGCAATCTACGTCCATTATTTTAAGGTGCTCGGATTCGGAAAAGCAACCTCCAGAGATGGCATCAGTCATGTCAATCCGACTTACTGGTCCTAGATCTTCTTTGATGACCTCTCCAATTCATGTCTCTCACCTAGGAGCTCAAGCATTTGCTTGGAGAACACAAATAATGTCACTTAAAGATTCACAAACTTACCCTGGACCATTTGTTATAGTTGACGTTTCAAGGAAATCCCAGGATGGTTTGTCAATTCTTATTTCTCCTCTCGTAAGAATACACAATGAAACTGGATTATCTATAAAGCTACGGTTTCGACGATCTCAGCAGAAGGAAGACGTGTTTGCTTCAGTGGTGCTTAATACTGGTGATACATATGATGACTCCATGGCCATGTTTGATGCTATAAATTTGTCTGGTGAAGAAAAGAAGGCCCTGAGGTCTTTAAGTCTTGGCAATTTCTTGTTCTCTTTTAGACCTGAAATTCCAGATGGCTTAATGAACTCTAAGAAATCAATTTCAGCAAAATGGTCAGATGATCTTAAAGGTGGAAAGGCAGTTCGTCTCTCTGGAATCTTTGATAAATTAAGTTATAAAGTGCGGAAGGCTTTGTTTAGTGAAACTGCGAAATCTTCCTTCAGCACCGCCCATTGTACTCTTAAATCGGAAGGGGCAAATACTGTCGACATGCATTTTTTGATTCAGAGTATAAGGAGAAATGTTCCTATTGTACAACCTGATAGGTCTATGAATCTCCTTGAAAATAGCAAATCACCAGTTGCCTTACAAGAGCAAAAGGATATTTATCTTCTTCCTACAGTGTGTGTTTCCAATTTATTGCATACGGAGATACATGTCTTTGTAAGTGAATCAGATGGACGTCCTACTATTGCGTCTGACAATGATAGGAATCAATCAAACATATCTTGTGGTTCCATGGTTGAATTTTATGCCAACCCTTCCATTATTTACTTCACAATCACACTGACTGGTTATGATGCAAGCTGCAAACCAGTGAACAGCAGTGATTGGGTCAAAAAGTTACTGAAGCAGAAAAGTGATGTGCCTTCTGTGGACATTGATCTGGATTTTGGTGGGGGAAAGTATTCTGCTACCTTAAGATTGTCTCGAGGGAGCAGAGGCACATTAGAGGCTGCTATTTTCACATCATACGCCCTGAAAAATGATACAGAATTTGGCCTTTGTTTCTTTGTGTCTAATAAGAGGCCCCTTTCTAGAGATGAAGCAGAGAAGTATGGTTCTAGCATTCCTTCAGAATTTGGATTATATTTGCCTCCCAAGTCAACCAGATCATGGTTCTTAAAATCCAGCAAAGTGCGTCTCAAATTGCTAAAAGATAATGCATCGGAGACACTTATCGACTTGGATGCTTTATCAGGACTTGCAGAGATAAGTTTGGAAACAGAGGAAGGATCTGGAATCAGATCTATTACAAAGCTTGGGGTTTCCACAGGACCACCTTTGAGTAAAGTGGTTGTGCCATCACATATAGTAACTATGGTCCCAAGACATGTTGTAATAAATGAATCAGGAGAAAACATCATTGTTCGCCAATGTTATTTGCAGGATGACACTGTTGGCATGATCCCCGTAAACAGTAAACAGAGGGCGCCTCTACAGCTGTGGAATGTGATAGACAAGAAGAGAGATGTCAGTTTGTTTGAGCATGTTATGAAAAAGCACAGAAAAGCTAATGATGATTCACCAATATATCTCCAGTTTCGATTGAATGAGTCTAAGTTAGGTTGGTCGGGGCCAGTATGCATTGCTTCTCTAGGAAGATTTTTCCTCAAGTTCAAAAGGCAGCAATTGGACCAAGTGACAGCACTAGAAAGCAATGTAACAGAATTCGCTTCTGTTCATCTTGTTGAAGAAGGCTCTACTCTTGGTTTACACTTCCACAAACCTCCAAACGTCAGTCTCCCATACCGAATTGAGAATTGCTTGCCTGATGTGTCGATAACTTACTACCAGAAGGATTCATCAGAGCCAGAGATTATTGGATCGGAAAGTTGCATTGATTATGTTTGGGATGATTTGACTCTTCCACATAAGTTGGTCGTCAGGATCAATGATAGTCTTCTACTACGTGAAATTAACTTGGATAAGGTGAGAGCATGGAAGCCTTTCTACAAAACCAGGCAACGGAGTGGATTGGCAACCCATTTACCATTTGGTAAAGACTCGGGGGACAAGAAGGGTGATTTTGGTGAGTTTAATGGCATGGAGATGATGAAGGTGGGGTTTGAAGTATATGCCGATGGGCCAACCCGGGTCTTAAGATTTTGTGAAATTTCTAGCAGCCACAAGGGAGATAAAATGTTTCAATCATGCCAGAAGATTCAACTTAGATTTAATCAGTTTACAGTTCACCTTCTTGAACATGAAAAGCAGGATGGAGAAGATATGGAGCCGCCAGGGTATACACCAATCTTAGCTGCAAGAATTGGGAATATTAACTTTGATTCTGTCTTTACATATGAGCAAAAGTTTAGCCAGATCAGTGTACAGTCATTGAATTTGGAGAACAAGTGGGTGGGTGCTCCTTTTGCAGCAATGCTTCGTAGACATCAATCAGACTTCGATGATTCAAATGATTGTGTACTCAAAATTGTCATTGTCTTGCTGTCAACCAGTTCCAATGTTGTACAAGTTAAGTACTCGTCTATTGCTCTGCAGCCAATGGATTTAAATCTTGATGAGGAGACGTTAATGAAAATTGTCCCATTTTGGAGAACATCTCTTAGTGAGGCGAAGAGTAGCCAGTATTATTTTGATCATTTTGAGATCCATCCGATAAAGATTATTGCAAACTTTCTTCCTGGCGAGTCATATTCAAGTTATAGCTCAGCTGAGGAGACTCTAAGGTCCTTGCTGCACAGTGTAGTTAAGGTCCCTGCAATAAAAAATAAGGTTGTAGAGCTCAATGGTGTTATGGTCACTCATGCTCTTATAACGATGCGTGAATTACTTATCAAATGCGCACAACATTATTCATGGTATGCCATGAGGGCTATATATATTGCAAAGGGAAGTCCATTGCTTCCACCAGATTTTGTGTCAATATTTGATGACTTGGCTTCTTCATCTCTTGACGTCTTCTTTGATCCTTCTCGTGCATTAGTGACTCTTCCAGGTTTGACTTTAGGTGTCATTGATACTATGAAGGACAATTACTGCAATGCCAATACTATGCTACAGTACTATAACTATAAAGCCAAGTCACCTATATTGAGGATGGCAGGGTCAAATGTATTGTTTGCTGCAGTCACTGAAATATCAGACTCTATTCTGAAAGGAGCAGAAGCAAGTGGTTTTGATGGAGTGGTCACGGGCTTTCACCATGGAATATTGAAATTGGCTATGGAGCCATCATTATTGGGAACTGCTTTGATGGAAGGTGGCCCAGATAGAAAGATTAAGCTTGACCGCAGTCCTGCTGTTGATGAGTTATATATTGAAGGATACTTGCAAGCGATGTTGGATACGATGTATAGACAAGAATATTTGAGAGTCAGAGTCATCGAGAATCAGGTTTACCTCAAAAACCTGCCCCCAAACAGTTCTCTTATAAACGAGATTATGGATCGTGTGAAGGGGTTTCTTGTGAGCAAGTCACTTCTGAAAGGAGACCCCTCAATAACATCTCGCCCCTTGGGTCATCTTCGAGGTGAACGTGAGTGGAGAATCGGCCCAACTGTGCTGACATTAGGTGAGCATCTGTTCGTGAGTTTCGCAATCCGAATCCTGCGGAAGCAAGCTAACAAGTGCATCGCCAACATCAAGTGGAAGCCAGAGAGCGATGGTGGTCGATCGATTGTCCCTGCAAGCTCCTCGGAAGAAGTGGTGAAGGGTAAGTTCATTTGGAAATGGGGAAGTGGGATCGGCAAGTTTGTTTTGTCAGCCATAGTAGCATATGTAGATGGACGGTTATGTCGTTCTATTCCTAATCCAGTAGCTCGCCGCATCGTTAGTGGCTTTCTGTTGACTTTCCTTGATAACAGCAATAGCAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHR-BD VPS13_C

Protein Analysis

3237

Amino Acids

361.91

Weight (kDa)

6.54

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_VAB PF25036 2157 - 2576 1.1e-19 Vacuolar-sorting associated protein 13, adaptor binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 5324, 6267, 8648, 9266
AasI GACNNNNNNGTC 4 cut(s) 3768, 5763, 8560, 9627
AatII GACGTC 3 cut(s) 4776, 6556, 8793
Acc16I TGCGCA 1 cut(s) 8677
Acc36I ACCTGC 2 cut(s) 683, 9252
AccB1I GGYRCC 1 cut(s) 7303
AccB7I CCANNNNNTGG 3 cut(s) 2443, 2578, 6987
AccBSI CCGCTC 2 cut(s) 38, 4183
AccI GTMKAC 6 cut(s) 765, 3790, 4103, 6357, 8117, 8260
AccII CGCG 2 cut(s) 107, 137
AccIII TCCGGA 1 cut(s) 808
AclI AACGTT 1 cut(s) 3942
AcoI YGGCCR 3 cut(s) 33, 2354, 6063
AcyI GRCGYC 5 cut(s) 258, 4773, 6553, 7304, 8790
AdeI CACNNNGTG 1 cut(s) 109
AfeI AGCGCT 1 cut(s) 4633
AflII CTTAAG 2 cut(s) 6790, 7972
AflIII ACRYGT 7 cut(s) 706, 1569, 1821, 2203, 6012, 6526, 7204
AjiI CACGTC 1 cut(s) 6013
AjuI GAANNNNNNNTTGG 8 cut(s) 498, 530, 1979, 2011, 3932, 3964, 4320, 4352
AloI GAACNNNNNNTCC 4 cut(s) 5451, 5483, 6216, 6248
Alw44I GTGCAC 2 cut(s) 2330, 2942
AlwNI CAGNNNCTG 5 cut(s) 2402, 2443, 3554, 5447, 8966
Ama87I CYCGRG 6 cut(s) 74, 122, 279, 6801, 7879, 7965
Aor13HI TCCGGA 1 cut(s) 808
Aor51HI AGCGCT 1 cut(s) 4633
ApaI GGGCCC 1 cut(s) 2394
ApaLI GTGCAC 2 cut(s) 2330, 2942
ArsI GACNNNNNNTTYG 2 cut(s) 8780, 8812
Asp700I GAANNNNTTC 5 cut(s) 1746, 4351, 4985, 6778, 7820
AspA2I CCTAGG 1 cut(s) 5809
AspLEI GCGC 7 cut(s) 142, 3523, 3875, 4634, 4740, 7306, 8678
AsuC2I CCSGG 3 cut(s) 3435, 7966, 7967
AsuNHI GCTAGC 2 cut(s) 895, 2138
AvaI CYCGRG 6 cut(s) 74, 122, 279, 6801, 7879, 7965
AvrII CCTAGG 1 cut(s) 5809
AxyI CCTNAGG 3 cut(s) 372, 2426, 6110
BaeGI GKGCMC 4 cut(s) 2334, 2394, 2946, 4804
BaeI ACNNNNGTAYC 2 cut(s) 3732, 3765
BalI TGGCCA 2 cut(s) 2356, 6065
BanI GGYRCC 1 cut(s) 7303
BanII GRGCYC 8 cut(s) 81, 647, 2394, 3440, 3915, 4863, 5818, 8619
BarI GAAGNNNNNNTAC 8 cut(s) 1569, 1601, 5668, 5700, 7808, 7840, 8852, 8884
BauI CACGAG 2 cut(s) 3914, 8808
BbsI GAAGAC 5 cut(s) 590, 3123, 6015, 7766, 8785
BbvCI CCTCAGC 2 cut(s) 2395, 8547
BcgI CGANNNNNNTGC 4 cut(s) 7043, 7077, 9510, 9544
BciVI GTATCC 3 cut(s) 316, 9152, 9172
BclI TGATCA 5 cut(s) 93, 1783, 3172, 4443, 8470
BcnI CCSGG 3 cut(s) 3435, 7966, 7967
BfoI RGCGCY 3 cut(s) 143, 4635, 7307
BfrI CTTAAG 2 cut(s) 6790, 7972
BfuAI ACCTGC 2 cut(s) 683, 9252
BfuI GTATCC 3 cut(s) 316, 9152, 9172
BglI GCCNNNNNGGC 1 cut(s) 8705
BglII AGATCT 3 cut(s) 1366, 5771, 7118
BlnI CCTAGG 1 cut(s) 5809
BlpI GCTNAGC 3 cut(s) 3496, 4594, 8542
BmcAI AGTACT 4 cut(s) 1877, 3169, 8354, 8897
BmeT110I CYCGRG 6 cut(s) 74, 122, 279, 6801, 7879, 7965
BmgBI CACGTC 1 cut(s) 6013
BmrI ACTGGG 3 cut(s) 3479, 4864, 5387
BmtI GCTAGC 2 cut(s) 899, 2142
BmuI ACTGGG 3 cut(s) 3479, 4864, 5387
BoxI GACNNNNGTC 1 cut(s) 9126
BpiI GAAGAC 5 cut(s) 590, 3123, 6015, 7766, 8785
BplI GAGNNNNNCTC 4 cut(s) 7291, 7323, 8419, 8451
BpmI CTGGAG 6 cut(s) 1303, 2599, 4246, 5055, 5712, 7394
Bpu10I CCTNAGC 4 cut(s) 2395, 2487, 3373, 8547
Bpu1102I GCTNAGC 3 cut(s) 3496, 4594, 8542
BpuEI CTTGAG 5 cut(s) 3424, 3941, 4032, 5802, 7469
BpuMI CCSGG 3 cut(s) 3435, 7966, 7967
Bsa29I ATCGAT 1 cut(s) 9509
BsaAI YACGTR 1 cut(s) 7784
BsaBI GATNNNNATC 8 cut(s) 861, 1621, 2005, 2453, 4545, 6477, 6582, 8385
BsaHI GRCGYC 5 cut(s) 258, 4773, 6553, 7304, 8790
BsaI GGTCTC 1 cut(s) 9318
BsaWI WCCGGW 2 cut(s) 808, 4453
BsaXI ACNNNNNCTCC 6 cut(s) 5451, 5481, 5606, 5636, 8210, 8240
Bse21I CCTNAGG 3 cut(s) 372, 2426, 6110
Bse8I GATNNNNATC 8 cut(s) 861, 1621, 2005, 2453, 4545, 6477, 6582, 8385
BseAI TCCGGA 1 cut(s) 808
BseCI ATCGAT 1 cut(s) 9509
BseJI GATNNNNATC 8 cut(s) 861, 1621, 2005, 2453, 4545, 6477, 6582, 8385
BseRI GAGGAG 5 cut(s) 4451, 5928, 8411, 8564, 9517
BseSI GKGCMC 4 cut(s) 2334, 2394, 2946, 4804
BseYI CCCAGC 1 cut(s) 2058
BsgI GTGCAG 4 cut(s) 751, 1383, 4623, 8555
Bsh1236I CGCG 2 cut(s) 107, 137
Bsh1285I CGRYCG 1 cut(s) 9509
BshNI GGYRCC 1 cut(s) 7303
BshVI ATCGAT 1 cut(s) 9509
BsiEI CGRYCG 1 cut(s) 9509
BsiHKCI CYCGRG 6 cut(s) 74, 122, 279, 6801, 7879, 7965
BsiSI CCGG 6 cut(s) 22, 68, 809, 3434, 4454, 7966
BsmBI CGTCTC 4 cut(s) 4780, 6242, 7019, 8393
BsmI GAATGC 4 cut(s) 236, 833, 4723, 6939
Bso31I GGTCTC 1 cut(s) 9318
BsoBI CYCGRG 6 cut(s) 74, 122, 279, 6801, 7879, 7965
Bsp120I GGGCCC 1 cut(s) 2390
Bsp13I TCCGGA 1 cut(s) 808
Bsp1407I TGTACA 4 cut(s) 3806, 6403, 8200, 8341
Bsp1720I GCTNAGC 3 cut(s) 3496, 4594, 8542
Bsp19I CCATGG 4 cut(s) 1677, 6060, 6609, 9037
BspDI ATCGAT 1 cut(s) 9509
BspEI TCCGGA 1 cut(s) 808
BspFNI CGCG 2 cut(s) 107, 137
BspHI TCATGA 1 cut(s) 5008
BspMAI CTGCAG 4 cut(s) 2665, 2758, 8373, 8962
BspMI ACCTGC 2 cut(s) 683, 9252
BspOI GCTAGC 2 cut(s) 899, 2142
BspQI GCTCTTC 2 cut(s) 57, 4173
BspT107I GGYRCC 1 cut(s) 7303
BspTI CTTAAG 2 cut(s) 6790, 7972
BspTNI GGTCTC 1 cut(s) 9318
BsrBI CCGCTC 2 cut(s) 38, 4183
BsrGI TGTACA 4 cut(s) 3806, 6403, 8200, 8341
BssNAI GTATAC 2 cut(s) 3791, 8118
BssNI GRCGYC 5 cut(s) 258, 4773, 6553, 7304, 8790
BssSI CACGAG 2 cut(s) 3914, 8808
BssT1I CCWWGG 7 cut(s) 1677, 5251, 5809, 6060, 6609, 9037, 9348
Bst1107I GTATAC 2 cut(s) 3791, 8118
Bst2BI CACGAG 2 cut(s) 3914, 8808
Bst6I CTCTTC 9 cut(s) 57, 1572, 3885, 4173, 5223, 7331, 7743, 8444, 8829
BstACI GRCGYC 5 cut(s) 258, 4773, 6553, 7304, 8790
BstAFI CTTAAG 2 cut(s) 6790, 7972
BstAPI GCANNNNNTGC 2 cut(s) 3028, 5048
BstAUI TGTACA 4 cut(s) 3806, 6403, 8200, 8341
BstBAI YACGTR 1 cut(s) 7784
BstDSI CCRYGG 5 cut(s) 135, 1677, 6060, 6609, 9037
BstFNI CGCG 2 cut(s) 107, 137
BstH2I RGCGCY 3 cut(s) 143, 4635, 7307
BstHHI GCGC 7 cut(s) 142, 3523, 3875, 4634, 4740, 7306, 8678
BstMCI CGRYCG 1 cut(s) 9509
BstPAI GACNNNNGTC 1 cut(s) 9126
BstSLI GKGCMC 4 cut(s) 2334, 2394, 2946, 4804
BstUI CGCG 2 cut(s) 107, 137
BstV2I GAAGAC 5 cut(s) 590, 3123, 6015, 7766, 8785
BstX2I RGATCY 9 cut(s) 571, 1366, 1616, 2496, 4757, 5771, 7106, 7118, 8481
BstYI RGATCY 9 cut(s) 571, 1366, 1616, 2496, 4757, 5771, 7106, 7118, 8481
BstZ17I GTATAC 2 cut(s) 3791, 8118
Bsu15I ATCGAT 1 cut(s) 9509
Bsu36I CCTNAGG 3 cut(s) 372, 2426, 6110
BsuI GTATCC 3 cut(s) 316, 9152, 9172
BsuTUI ATCGAT 1 cut(s) 9509
BtgI CCRYGG 5 cut(s) 135, 1677, 6060, 6609, 9037
BtgZI GCGATG 4 cut(s) 3655, 9185, 9451, 9510
BtrI CACGTC 1 cut(s) 6013
BtsI GCAGTG 4 cut(s) 670, 2893, 5269, 6703
BveI ACCTGC 2 cut(s) 683, 9252
CaiI CAGNNNCTG 5 cut(s) 2402, 2443, 3554, 5447, 8966
CciI TCATGA 1 cut(s) 5008
CfoI GCGC 7 cut(s) 142, 3523, 3875, 4634, 4740, 7306, 8678
Cfr42I CCGCGG 1 cut(s) 138
Cfr9I CCCGGG 1 cut(s) 7965
ClaI ATCGAT 1 cut(s) 9509
CseI GACGC 5 cut(s) 247, 1550, 4089, 6555, 7001
CsiI ACCWGGT 1 cut(s) 2083
CspCI CAANNNNNGTGG 2 cut(s) 7731, 7766
DinI GGCGCC 1 cut(s) 7305
DraI TTTAAA 3 cut(s) 1909, 4323, 8385
DraIII CACNNNGTG 1 cut(s) 109
DrdI GACNNNNNNGTC 4 cut(s) 3768, 5763, 8560, 9627
DseDI GACNNNNNNGTC 4 cut(s) 3768, 5763, 8560, 9627
EaeI YGGCCR 3 cut(s) 33, 2354, 6063
Eam1104I CTCTTC 9 cut(s) 57, 1572, 3885, 4173, 5223, 7331, 7743, 8444, 8829
EarI CTCTTC 9 cut(s) 57, 1572, 3885, 4173, 5223, 7331, 7743, 8444, 8829
EciI GGCGGA 2 cut(s) 40, 5214
Ecl136II GAGCTC 5 cut(s) 79, 645, 3913, 5816, 8617
Eco130I CCWWGG 7 cut(s) 1677, 5251, 5809, 6060, 6609, 9037, 9348
Eco147I AGGCCT 1 cut(s) 609
Eco24I GRGCYC 8 cut(s) 81, 647, 2394, 3440, 3915, 4863, 5818, 8619
Eco31I GGTCTC 1 cut(s) 9318
Eco32I GATATC 3 cut(s) 1593, 2455, 4701
Eco47III AGCGCT 1 cut(s) 4633
Eco53kI GAGCTC 5 cut(s) 79, 645, 3913, 5816, 8617
Eco81I CCTNAGG 3 cut(s) 372, 2426, 6110
Eco88I CYCGRG 6 cut(s) 74, 122, 279, 6801, 7879, 7965
EcoICRI GAGCTC 5 cut(s) 79, 645, 3913, 5816, 8617
EcoRI GAATTC 2 cut(s) 4672, 7541
EcoRV GATATC 3 cut(s) 1593, 2455, 4701
EcoT14I CCWWGG 7 cut(s) 1677, 5251, 5809, 6060, 6609, 9037, 9348
EcoT22I ATGCAT 3 cut(s) 6366, 7039, 7460
EcoT38I GRGCYC 8 cut(s) 81, 647, 2394, 3440, 3915, 4863, 5818, 8619
EgeI GGCGCC 1 cut(s) 7305
EheI GGCGCC 1 cut(s) 7305
ErhI CCWWGG 7 cut(s) 1677, 5251, 5809, 6060, 6609, 9037, 9348
Esp3I CGTCTC 4 cut(s) 4780, 6242, 7019, 8393
FalI AAGNNNNNCTT 4 cut(s) 3087, 3119, 4577, 4609
FauNDI CATATG 4 cut(s) 4554, 6049, 8173, 9610
FbaI TGATCA 5 cut(s) 93, 1783, 3172, 4443, 8470
FblI GTMKAC 6 cut(s) 765, 3790, 4103, 6357, 8117, 8260
FriOI GRGCYC 8 cut(s) 81, 647, 2394, 3440, 3915, 4863, 5818, 8619
FspAI RTGCGCAY 1 cut(s) 8677
FspI TGCGCA 1 cut(s) 8677
GlaI GCGC 7 cut(s) 141, 3522, 3874, 4633, 4739, 7305, 8677
GsaI CCCAGC 1 cut(s) 2062
GsuI CTGGAG 6 cut(s) 1303, 2599, 4246, 5055, 5712, 7394
HaeII RGCGCY 3 cut(s) 143, 4635, 7307
HapII CCGG 6 cut(s) 22, 68, 809, 3434, 4454, 7966
HgaI GACGC 5 cut(s) 247, 1550, 4089, 6555, 7001
HhaI GCGC 7 cut(s) 142, 3523, 3875, 4634, 4740, 7306, 8678
Hin1I GRCGYC 5 cut(s) 258, 4773, 6553, 7304, 8790
Hin6I GCGC 7 cut(s) 140, 3521, 3873, 4632, 4738, 7304, 8676
HinP1I GCGC 7 cut(s) 140, 3521, 3873, 4632, 4738, 7304, 8676
HincII GTYRAC 8 cut(s) 698, 1540, 4771, 5893, 6358, 6978, 8325, 9681
HindII GTYRAC 8 cut(s) 698, 1540, 4771, 5893, 6358, 6978, 8325, 9681
HindIII AAGCTT 9 cut(s) 311, 353, 404, 3840, 3980, 5099, 5422, 7130, 9116
HpaII CCGG 6 cut(s) 22, 68, 809, 3434, 4454, 7966
Hpy99I CGWCG 3 cut(s) 161, 4755, 5994
Hsp92I GRCGYC 5 cut(s) 258, 4773, 6553, 7304, 8790
HspAI GCGC 7 cut(s) 140, 3521, 3873, 4632, 4738, 7304, 8676
KasI GGCGCC 1 cut(s) 7303
Kpn2I TCCGGA 1 cut(s) 808
Ksp22I TGATCA 5 cut(s) 93, 1783, 3172, 4443, 8470
KspI CCGCGG 1 cut(s) 138
LguI GCTCTTC 2 cut(s) 57, 4173
MabI ACCWGGT 1 cut(s) 2083
MbiI CCGCTC 2 cut(s) 38, 4183
MfeI CAATTG 4 cut(s) 243, 4871, 5599, 7502
MflI RGATCY 9 cut(s) 571, 1366, 1616, 2496, 4757, 5771, 7106, 7118, 8481
MlsI TGGCCA 2 cut(s) 2356, 6065
MluNI TGGCCA 2 cut(s) 2356, 6065
Mly113I GGCGCC 1 cut(s) 7304
MmeI TCCRAC 6 cut(s) 220, 1809, 3930, 4318, 5779, 9156
Mox20I TGGCCA 2 cut(s) 2356, 6065
Mph1103I ATGCAT 3 cut(s) 6366, 7039, 7460
MroI TCCGGA 1 cut(s) 808
MroXI GAANNNNTTC 5 cut(s) 1746, 4351, 4985, 6778, 7820
MscI TGGCCA 2 cut(s) 2356, 6065
MslI CAYNNNNRTG 7 cut(s) 1156, 1452, 2243, 4122, 5018, 6065, 7922
Msp20I TGGCCA 2 cut(s) 2356, 6065
MspA1I CMGCKG 6 cut(s) 137, 956, 1139, 4814, 7315, 8546
MspCI CTTAAG 2 cut(s) 6790, 7972
MspI CCGG 6 cut(s) 22, 68, 809, 3434, 4454, 7966
MunI CAATTG 4 cut(s) 243, 4871, 5599, 7502
Mva1269I GAATGC 4 cut(s) 236, 833, 4723, 6939
MvnI CGCG 2 cut(s) 107, 137
NarI GGCGCC 1 cut(s) 7304
NciI CCSGG 3 cut(s) 3435, 7966, 7967
NcoI CCATGG 4 cut(s) 1677, 6060, 6609, 9037
NdeI CATATG 4 cut(s) 4554, 6049, 8173, 9610
NheI GCTAGC 2 cut(s) 895, 2138
NmeAIII GCCGAG 1 cut(s) 132
NsbI TGCGCA 1 cut(s) 8677
NsiI ATGCAT 3 cut(s) 6366, 7039, 7460
PaeI GCATGC 2 cut(s) 392, 4712
PaeR7I CTCGAG 2 cut(s) 74, 6801
PagI TCATGA 1 cut(s) 5008
PceI AGGCCT 1 cut(s) 609
PciI ACATGT 5 cut(s) 1569, 1821, 2203, 6526, 7204
PciSI GCTCTTC 2 cut(s) 57, 4173
PcsI WCGNNNNNNNCGW 3 cut(s) 5989, 9367, 9628
PctI GAATGC 4 cut(s) 236, 833, 4723, 6939
PdmI GAANNNNTTC 5 cut(s) 1746, 4351, 4985, 6778, 7820
PflMI CCANNNNNTGG 3 cut(s) 2443, 2578, 6987
PfoI TCCNGGA 1 cut(s) 4165
Ple19I CGATCG 1 cut(s) 9509
PluTI GGCGCC 1 cut(s) 7307
Ppu21I YACGTR 1 cut(s) 7784
PpuMI RGGWCCY 6 cut(s) 2910, 4009, 4163, 4884, 8562, 8587
PscI ACATGT 5 cut(s) 1569, 1821, 2203, 6526, 7204
PshAI GACNNNNGTC 1 cut(s) 9126
PsiI TTATAA 4 cut(s) 5324, 6267, 8648, 9266
Psp124BI GAGCTC 5 cut(s) 81, 647, 3915, 5818, 8619
Psp1406I AACGTT 1 cut(s) 3942
Psp5II RGGWCCY 6 cut(s) 2910, 4009, 4163, 4884, 8562, 8587
PspFI CCCAGC 1 cut(s) 2058
PspOMI GGGCCC 1 cut(s) 2390
PspPPI RGGWCCY 6 cut(s) 2910, 4009, 4163, 4884, 8562, 8587
PspXI VCTCGAGB 1 cut(s) 74
PstI CTGCAG 4 cut(s) 2665, 2758, 8373, 8962
PstNI CAGNNNCTG 5 cut(s) 2402, 2443, 3554, 5447, 8966
PsuI RGATCY 9 cut(s) 571, 1366, 1616, 2496, 4757, 5771, 7106, 7118, 8481
PvuI CGATCG 1 cut(s) 9509
PvuII CAGCTG 4 cut(s) 956, 1139, 7315, 8546
RseI CAYNNNNRTG 7 cut(s) 1156, 1452, 2243, 4122, 5018, 6065, 7922
SacI GAGCTC 5 cut(s) 81, 647, 3915, 5818, 8619
SacII CCGCGG 1 cut(s) 138
SalI GTCGAC 1 cut(s) 6356
SapI GCTCTTC 2 cut(s) 57, 4173
ScaI AGTACT 4 cut(s) 1877, 3169, 8354, 8897
SexAI ACCWGGT 1 cut(s) 2083
SfoI GGCGCC 1 cut(s) 7305
Sfr274I CTCGAG 2 cut(s) 74, 6801
Sfr303I CCGCGG 1 cut(s) 138
SgrBI CCGCGG 1 cut(s) 138
SlaI CTCGAG 2 cut(s) 74, 6801
SmaI CCCGGG 1 cut(s) 7967
SmiI ATTTAAAT 1 cut(s) 8385
SmiMI CAYNNNNRTG 7 cut(s) 1156, 1452, 2243, 4122, 5018, 6065, 7922
SmlI CTYRAG 9 cut(s) 74, 3439, 3920, 4047, 5817, 6790, 6801, 7484, 7972
SmoI CTYRAG 9 cut(s) 74, 3439, 3920, 4047, 5817, 6790, 6801, 7484, 7972
SphI GCATGC 2 cut(s) 392, 4712
SseBI AGGCCT 1 cut(s) 609
SspDI GGCGCC 1 cut(s) 7303
SstI GAGCTC 5 cut(s) 81, 647, 3915, 5818, 8619
StuI AGGCCT 1 cut(s) 609
StyI CCWWGG 7 cut(s) 1677, 5251, 5809, 6060, 6609, 9037, 9348
SwaI ATTTAAAT 1 cut(s) 8385
TauI GCSGC 7 cut(s) 15, 38, 49, 140, 2552, 8110, 9660
TspGWI ACGGA 4 cut(s) 1380, 3356, 6533, 7855
TspMI CCCGGG 1 cut(s) 7965
Van91I CCANNNNNTGG 3 cut(s) 2443, 2578, 6987
Vha464I CTTAAG 2 cut(s) 6790, 7972
VneI GTGCAC 2 cut(s) 2330, 2942
XbaI TCTAGA 1 cut(s) 6908
XcmI CCANNNNNNNNNTGG 1 cut(s) 7865
XhoI CTCGAG 2 cut(s) 74, 6801
XmaI CCCGGG 1 cut(s) 7965
XmaJI CCTAGG 1 cut(s) 5809
XmiI GTMKAC 6 cut(s) 765, 3790, 4103, 6357, 8117, 8260
XmnI GAANNNNTTC 5 cut(s) 1746, 4351, 4985, 6778, 7820
ZraI GACGTC 3 cut(s) 4774, 6554, 8791
ZrmI AGTACT 4 cut(s) 1877, 3169, 8354, 8897
Zsp2I ATGCAT 3 cut(s) 6366, 7039, 7460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.