Rw2G012750

regulation of parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004207
Physical Location & Seq
Reverse (-)
13274738 .. 13294441
19704 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G012750.1

Sequence Viewer

Length: 9564 bp
ATGTTTCCGAGCGGCACAATCCGGCGGAGGCTGGCCGCTCTGCTGCGGCCGTGGCTTCGGGAAGAGCCGGAACTCGAGCTCAGTTTGGGGCTGATCAGTTCGCACGCGGTGGCTAAGAACCTCCGGTTCGATACCGCGGCGCTCAATCGGCTCGGCGACGATTCGGACGGGTTTTTGTATAAGGCGGTCAGTGTCGAACAGTTGAGCGTTCGGTTCTCGAACTGGCTCGCTCCTGCATTCTCAATTGAGTTTCATGGCGTCAAGGTGGTGCTCTCGACTCGCTTGAATTTTTTTTTTTTTTTAAATTTCAAGGGAGTTGAAGAAGAAGAAGAGAAGAAGCTTTCTGAGGATACATTTGCAGAGGATATGAAGAAGAAGAAGCTTTCTGAGATTGATCCTCAGGGGAGTGCTGTGCATGCTTTTCTGGAAAAGCTTATGGCTACTTCTACGACTCCGAAAAATAAGTTCAAAACATCTCTTTTGAATCTGTTACTCACACACTGTCAGCTTCAAGTGCATGATATTAGTGTCCAAGTCCAAGTTCCGATCTTAAATGATTTGTTTTTCTGTTCACTGGATATAGAAGATATAAATGTAGATCCTCAGTATATTGATCGTGGGTGTCTTCTTCGAGGCCTTTTTGGTACATTCTTTGTACCAATGAAGGAGAGCTCTTTTGCTATAGTAGGCAGTGGTTGTGAGGTTGGTTTTAAGAGGGCAGGTCAACTCAAACGTGTCTTGTTATTGACCGAGTTGTGCAGTTGCATTAAGTTGAATGATTTTCAGCTTGTAGACATCAATCTTCGAGTCCCTGAAATACATTTTTCGTTTTCTCCGGATGATGTTTCTTTCTATTTGGCATTCACTACAGCATCATCCCAAGAATCTCATCGTGCTAGAGATGGCAGACAACTGTGGAAGCTAGCTGCGAGTAGAATTGACAATATGACTTCAGCTCATAGGTGGTCGTTGCAAAAGTCAGCTGTTGCTGTTTGTTTCTGGTTACGTTATCTCAATGCTTATGAGTATTTACTGTTGCTAATTGGATATTATGACGACCACTTATTAAAAAGATCAGCTATTAGAATCTATGAGGACAAGATGCTTTTGAGTTCTGTTGAAAACCAATGGAAGGTGATTTCTGATATTGAGAAAGAGCTGCCAGCTGAAGCTATTGCACAGGCATGGAGAGTAGCCCGTAACAGAGCAGCATCAAATGTTCAATGTCCCGAATATAGTTCCAAAAAACCATTTGTGACTACAATTTACAACTTCCTTCTCATAATCCTTTCATTTCTAGCTTGTGCCTGGAGATTTTTTTGCAAGATAGTCCTTTTTATTATGCACCCCCTTGTTTTTAGGAAAATATTAGTCAATGAGCCAAAGAGTGCAGATCTGGATATTGTTTCTGAAGGTCCGTGTACGCAGTTTTGCTTCAGTTTGATGCTTGGAAAAGTTCAAATCACTATCTCTCATAGAAATGAAATTCAACTCTTAGTAAATGAAAAGTTGAAATCACATCTTGGGATCACTTACTCGGATTCACTTTCATTTCGTCTTTCAGTTGATGCACTGTTATTAAAATATGTAGAGGACATGTGTGAAGAGTCTTTACTGATATCCTGTGGGCAATTAAAGGTCAGATCCTCATCTCTTATGGAAGCTCCTGTTAAAGAAAGCAGTTCAAAATTATCCTTTAGTTCTATGGAAGCACATTGGAAGGAGAGCAATGATAAGTGGAACAATATTCTGTGGGGTGAGCCAGCAGAAATATTTCCACTTTTAGAAACTTCTGAAACTGGTTGTGCTGATCATGTTGAAGGTTCTTGTGTCTCATTTCTGAAAGACATGTGGTTGGATTGGAGAAGTGAGTGCGATAAATTTGGAAAAAGTGAAATTCAGTACTCTGAAACTCCATTCCTTCTTTGTGAATTTAAAAATTTCTTGATATATCCAGACCTCAAAACATCGGATTCTGGTTTTTGGAAGTTCTATTTTGTTCTGGGAAAGTTGAATCTTGTGTTGGGATATTCATCAATAGTATCATTGTCTTTGCTATTTAGGCAGGTACAACATACTCTATGCTGGGGAGAGGCCAATGGTCAGTCACCCGGTCTTTCACATTCTCCAAGAACCAGTGAGAGTAAACCAGAAATCAGTCTGGATAGCAAATACAAGTGCTATGCTAATAGATTAGAAATGGCATTGCTTAAAATGCTTCCAAAAAAACATGTTCAACTAGGGGTATTCATTGCTGACAAAAATCATGTATATATTCAGGACGATTTTCACGTTGCATTTGATGTGCACAATATTGAAGCTGCTGTTTGGCCAACCTCAAAGTTTGATCTTGCATCATTCGTGGGGCCCTCAGCATCTGATGATGTGGAACCAGAGTGCCTCAGGATGGACCAGCCTCTGGTGATTGATATCTCTAACTCTAATAATGGGAAGTATCAAGCTCAGGGTGAGATCTCTCTTGGTTCCTACATACGAGTTGATGGTTTAGATGTCTGCCTGGTTGCCGCAGCAGGAAAACGACAAAGCCAGATTTTGGTTTCTAAGCCAATGACTTTGCAGTTGTTATCCTCCAGGGAGTATGTTCATTCATTTAGCACAAATGTTATTGCTTTCTCTGCAGCTTTGTTTGGGAAAACTGAAGGATTCACCATTTTATCGTACATGGATGAATTTGATGTTCTCTTTCAGGTGCTTGCAAATTTATCTTCTGCAGTATCATATTCATTTAGACATTTTGCATCTGTCAGTGACTTGCCATTACAATTTGGAAAGCAACAATATTCAAATGCTAAATCTGAGAACGAGGAGACTACAGCGCATGAACCTCCCTTGAATTATAGCAGTGTCCTATTTTCAACTAATGGGACCTTCAAAATCAAGTCAATGAATATAATCGTGCACAAGTCTAGGATGGGTGATAATGTGGGGAGCTGCCTAGCAACCTCTGATGCTTCAAGTAGCAAACAGTTGTCTGAGCATGACTTGCCTGATTATGGAATTTGGATTTCAGTTCATCAAACAAGTCTTGATATGTCTTACAAAGAAGGAAAGGTGATACTTCTTTCTAATTTATCAGAAATTCAGTCTTCAAGTTTTAGATATAAGAATCGGAGAGGCAAAAGTACTGATCAGTCTGTACTTAGTGATCTGCTACTGCAATCTCTTGATTGCATATATCAACTATCACTTTCTAGTTGTGTACTCAGTTTGTCACTGTTCCTATCTCAGAACTGCTCATCTATTGGCACTGTTAGCAACACACTTGATACTAATTCTGTGAGTGAAACAGAGCACACGGAAAATTTCCCATTCACTAATTCTGAAAGCTCAGGTCATCAGAACTATAGTTTTATTCAAGGGTCAGAATTGGTTTCAAATATTCGACCTCCGGGCTCAAGCCATTGGTTACTTGTAAATCTTGCACTGGGTAAGATTTATGTGGGAAGGTGCTCAGCAAAGAATGTTATGAATGAGGCGCATCAATTAAATAAGTTTTTGTCATCAGTTTCTGTTGGTGGTGAATTTCAGAGAATCAGTTGTGAAATTCAGGGTGGTTTCCTTTTCCTTGAAACAACAGCTTCGGCTACCTTATTGCGATGTTTTTCTTCATATCTTGATTGCTTCACAAATATTTTATCTGGCTCACAATCATCCGATAAGCACGTTGAAGAAGATAAGCCCATTGAAGAAGCTGATACTGCTGTAAATATGACTAGACCAAATAGTCATTCTGATAAGGACTGTATGCAAGAGACTCCTTGTACATCTCCACAGGTCAAAGGGGGACAGATGGAAGCTTTTAGCTTAAATATATCTCGTTTTTCTTGCGCTCTTGTGATTCAGGAAGAGAATGGTGCTGTACAGGAGCTTGTGCTTGAGATTGATGCCCATTTGAACGTTGAGTTGGAAAACATGCTAAGAAAATTTATATTTAAGCTTTCTCGCATATCAATCCTTTCTCAGGTCCTTCAAGAAATTTTGGAAAATAAAACCCAAAGTTCTCAAGTTTCTTCAGTCACATCGAATACTTTTCAATCTCATGCTGCATCAGGAGCGTCTACAGGTTCTCAGCACATGGATGAGATTCATCCTGTTAATAATGCAAGTAGTTCAAGACGTCCTGGATCACAGGAAGAGCACTCTGAACATAGTTCTCTGCATGAGGTATTTCGTCTAAGTCATCACAAATATATCCTAAAGGGTCAAGAACTAGCCTCCAGTGAGTGCGAGTTAAGACAGGAAGGGGGAACTGCCTTCATATCAGTTGAGAAGCCTTTAAATGAAGTTTGGGTTGGAAGCGGAACTATTTCATGTTTTGATATTACAATATCTCTATCTCAAATACAGATGCTTTTGTCTATGATTTCATCCTTTTCTGGGGTATTTGGGGAGGAGTTGATCAGTGAACCGGATAGAAGGCACTGGTCTAATGACGAGGAATTAAAAAACAGTTTGGAGACAATGGTTCCTAATGGTGCAATAGTGGCAATTCAGGATGTCCATCAACATATGTATTTTACAGTTGAGGGTAATGAGGATAAGTACAGCTTAGCGGGTGCAGTTCATTATTCTCTTGTGGGAGAAAGCGCTCTTTTCATGGTCAAGTATAACAATCAAAAGGGATGGAATTCATCAGTTTTGTGGTTCTCCTTGATATCGTTGCATGCCAAGAATGCTTCTGGGGAGCCATTGCGCTTGAACTACCGTCGAGGATCTGATTTTGTTGACGTCTCTAGTGCGAATGATAATGCAGGGGCACATTGGACAGCGGTTTCTTGTGAGCCTGAAAGTTATGAGGGCGACATTGACTGGGAGCCCTACAATCAATTGGTTAAAAGGACCTTTTACCTTGTAAACAAAAAGAATGACTCTGCTGTTGCTATTGTAGATGGGATTCCAGAGTTTGTTAGAAAGCCTGGAAATCCAATCAAGTTGAAAGTGTTCCACAATGCTCCTACAGCTCATGACATAAAGATGGATAACTACCCTGGAGAGGCATCTGTGGCTCGTCCACAACAGAGTGATGAAGGGATAACTTCTGGGAAGGGTGGAAAGCTTCCCTGCATTGATGTTACATTTGACACAATTTCCTTGACCATTATCCATGAACTCGTAGATACAAAAGACATGCCTCTCCTTCGTTGTTGCATTGGCAGAACTGGACAGTCCAAACATGAACTTGAAGAGTCAAAAGACATGGCTCTCCTTGGTGGTTGCAGTGACAGAACCGAACGATCCAAACTTACTGTTCAAATTCTCCCATCTAAAGTTAGGATTATAAGCTCCTTAACAACAGTGGTATATTACTTTGATGCACAAAGAAACAAATGGAGAGAACTTATCCACCCAGTTGAAACATGCCTTTTCTATCGTTCAAGCTTCCAAATTCAGAGTTCAGAAGCTGTTTCGCATGGAGTACCTGTTCATATCCATTGCAGAACTAAAGAGTTGAATATATCCCTATCCGAACTTTCATTGGACATACTTCTCTTTACAGTTGGGAAACTAAATTTAGCTGGTCCTTTCTCAGTGAGAAGTACTAAGATTTGGGCCAATTGTTGCAAGGTGGAGAACCAGTCAGGATTAAACCTTCTTTGTCAGTGTGATGAGCAAAGTGTAAAAGTTTCCAGAAGGCAGTCTACGTCCATTATTTTAAGGTGCTTGGATTCGGAAAACCAACCTCCAGAGATGGCATCAGTCATGTCAATCCGACTTACTGGTCCTAGATCTTCTTTGATGACCTCTCCAATTCATGTCTCTCACCTAGAAGCTCAAGCATTTGCTTGGAGAACACAAATAATGTCACTTAAAGATTCACAAACTTACCCTGGACCATTTGTTATAGTTGACGTTTCAAGGAAATCCCAGGATGGTTTGTCAATTCTTATTTCTCCTCTCGTAAGAATACACAATGAAACTGGATTGTCTATAAAGCTACGATTTCGACGATCTCAGCAGAAGGAAGACGTGTTTGCTTCAGTGGTGCTTAATGCTGGTGATACATATGATGACTCCATGGCCATGTTTGATGCTATAAATTTGTCTGGTGAAGAAAAGAAGGCCCTGAGGTCTTTAAGTCTTGAAATCATTTCAGCAGAATGGTCAGATGATCTTAAAGGTGGAAAGGCAGTTCGTCTCTCTGGAATCTTTGATAAATTAAGCTATAAAGTGCGGAAGGCTTTCTTTAGTGAATCTGCGAAATCTTCCTTCAGCACTGCCCATTGTACTCTTAAATCGGAAGGGGCAAATACTGTCGACATGCATTTTTTGATTCAGAGTATAAGGAGAAATGTTCCTATTGTACAACCTGATAGGTCTATGAATCTCCTTGAAAATAGCAAATCACCAGTTGCCTTACAAGAGCAAAAGGATATTTATCTTCTTCCTACAGTGTGTGTTTCCAATTTATTGCATACGGAGATACATGTCTTTGTCAGTGAATCAGATGGACGTCCTACTATTGCGTCTGACAATGATAGGAATCAATCAACCATATCTTGTGGTTCCATGGTTGAATTTTATGCCAACCCTTCGATTATTTACTTCACAATCACACTGACTGGTTATGATGCAAACTGCAAACCAGTGAACAGCAGTGATTGGGTCAAAAAGTTACTGAAGCAGAAAAGTGATGTGCCTTCTGTGGACATTGATCTGGATTTTGGTGGGGGAAAGTATTCTGCTACCTTAAGATTGTCTCGAGGGAGCAGAGGCACATTAGAGGCGGCTATTTTCACATCATACTCCCTGAAAAATGATACAGAATTTGGCCTTTGTTTCTTTGTGTCTAATAAGAGGCCCCTTTCTAGGGATGAAGCAGAGAAGTATGGTTCTAGCATTCCTTCAGAATTTGGATTATATTTGCCTCCAAAGTCAACCAGATCATGGTTCTTAAAATCCAGCAAAGTGCGTCTCAAATTGCTAAAAGATAATGCATCGGAGACGCTTATCGACTTGGATTCTTTATCAGGACTTGCGGAGATAAGTTTGGAAACAGAGGAAGGATCTGGAATCAGATCTATTACAAAGCTTGGGGTTTCCACAGGACCACCTTTGAGTAAAGTGGTTGTGCCATCACAAATAGTAACTATGGTCCCAAGACATGTTGTAATAAATGAATCAGGAGAAAACATCATTGTTCGCCAATGTTATTTGCAGGATGACACTGTTGGCATGATCCCTGTCAACGGTAAACAGAGGGCGCCTCTACAGCTGTGGAATGTGATAGACAAGAAGAGAGATGTCAGTTTGTTTGAGCGTGTTATGAAAAAGCACAGAAAAGCTAATGATGATTCACCAATATATCTCCAGTTTCGATTGAATGAGTCTAAGTTAGGTTGGTCGGGGCCAGTATGCATTGCTTCTCTAGGAAGATTTTTCCTCAAGTTCAAAAGGCAGCAATTGGACCAAGTGACAGCACTAGAAAGCAATGTCACAGAATTCGCTTCTGTTCATCTTGTTGAAGAAGGCTCTACTCTTGGTTTACACTTCCATAAACCTCCAAATGTCAGTCTCCCATACCGAATTGAGAATTGCTTGCCTGATGTGTCGATAACTTACTACCAGAAGGATTCATCAGAGCCAGAGATTATTGCATCAGAAAGTTGCACTGATTATGTTTGGGATGATTTGACTCTTCCACATAAGTTGGTCGTCAGGATCAATGATAGTCTTCTACTACGTGAAATTAACTTGGATAAGGTGAGAGCATGGAAGCCTTTCTACAAAACCAGGCAACGGAGTGGATTGGCAACTCATTTACCATTTGGAAAAGACTCGGGGGACAAGAAGGGTGATTTTGGTGAGTTTAATGGCATGGAGATGATGAAGGTGGGGTTTGAAGTATATGCAGATGGTCCAACCCGGGTCTTAAGATTTTGTGAAATTTCTAGCAGCCACAAGGGAGATAAAATGTTTCAATCATGCGAAAAGATTCAACTAAGACTTAATCAGTTTACAATTCACCTTCTTGAACATGAAAAGCAGGATGGAGAAGATATGGAGCCGCCTGGGTATACACCAATCTTAGCTGCAAGAATTGGGAATATTAACTTTGATTCGGTCTTTACATATGAGCAAAAGTTTAGCCAGATCAGTGTACAGTCATTGAATTTGGAGAACAAGTGGGTGGGTGCTCCTTTTGCAGCAATGCTTCGTAGACATCAATCAGACTTCGATGATTCAAATGATTGTGTACTCAAAATTGTCATTGTCTTGCTGTCAACCAGTTCCAATGTTGTACAAGTTAAGTACTCGTCTATTGCTCTGCAGCCAATGGATTTAAATCTTGATGAGGAGACATTAATGAAAATTGTCCCATTTTGGAGAACATCTCTTAGTGAGGCGAAGAGTAGCCAGTATTATTTTGATCATTTTGAGATCCATCCGATAAAGATTATTGCAAACTTTCTTCCTGGCGAGTCATATTCAAGTTATAGCTCAACTGAGGAGACTCTAAGGTCCTTGCTGCACAGTGTAGTTAAGGTCCCTGCGATAAAAAATAAGGTTGTAGAGCTCAATGGTGTTATGGTCACTCATGCTCTTATAACGATGCGTGAATTACTTATCAAATGTGCACAACATTATTCATGGTATGCCATGAGGGCTATATATATTGCAAAGGGAAGTCCATTGCTTCCACCAGATTTTGTGTCAATTTTTGATGACTTGGCTTCTTCGTCTCTTGATGTCTTCTTTGATCCTTCTCGTGCATTAGTGACTCTTCCAGGTTTGACTTTAGGTACCTTCAAGTTAATCAGTAAATGTATCGATGGTAAAGGATTCTTGGGGACAAAACGCTATTTTGGTGACCTAGGAAAATCTTTGAGGACGGCAGGGTCAAATGTATTGTTTGCTGCAGTCACTGAAATATCAGACTCTATTCTGAAAGGAGCAGAAGCAAGTGGTTTTGATGGAGTGGTCACGGGCTTTCACCATGGAATATTGAAATTGGCTATGGAGCCATCATTATTGGGAACTGCTTTGATGGAAGGTGGCCCAGATAGAAAGATTAAGCTTGACCGCAGTCCTGCTGTTGATGAGTTATATATCGAAGGATACTTGCAAGCGATGTTGGATACGATGTATAGACAAGAATATTTGAGAGTCAGAGTCATCGAGAATCAGGTTTACCTCAAAAACCTGCCCCCCAACAGTTCCCTTATAAACGAGATTATGGATCGTGTGAAGGGGTTTCTTGTGAGCAAGTCACTTCTGAAAGGAGACCCCTCAATAACATCTCGCCCCTTGGGTCATCTTCGAGGTGAACGTGAGTGGAGAATCGGCCCAACTGTGCTGACATTAGGTGAGCATCTATTCGTGAGTTTCGCAATCCGAATCCTGCGGAAGCAAGCTAACAAGTGCATCGCCAACATCAAGTGGAAGCCAGAGAGCGATGGTGGTCGATCGATTGTCCCTGCAAGCTCCTCGGAAGAAGCGGTGAAGGGTAAGTTCATTTGGAAATGGGGAAGTGGGATCGGCAAGTTTGTTTTGTCAGCCATAGTAGCATATATAGATGGACGGTTATGTCGTTTATATTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHR-BD VPS13_C

Protein Analysis

3187

Amino Acids

356.76

Weight (kDa)

6.22

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_VAB PF25036 2130 - 2549 4.4e-19 Vacuolar-sorting associated protein 13, adaptor binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 5300, 8567, 9185
AasI GACNNNNNNGTC 5 cut(s) 3744, 5739, 8479, 8857, 9546
AatII GACGTC 3 cut(s) 4141, 4752, 6475
Acc36I ACCTGC 3 cut(s) 710, 2056, 9171
Acc65I GGTACC 1 cut(s) 8762
AccB1I GGYRCC 2 cut(s) 7222, 8762
AccB7I CCANNNNNTGG 3 cut(s) 2419, 2554, 6906
AccBSI CCGCTC 2 cut(s) 12, 38
AccI GTMKAC 6 cut(s) 792, 4079, 5660, 6276, 8036, 8179
AccII CGCG 2 cut(s) 107, 137
AccIII TCCGGA 1 cut(s) 835
AclI AACGTT 1 cut(s) 3918
AcoI YGGCCR 4 cut(s) 33, 47, 2330, 6039
AcyI GRCGYC 5 cut(s) 258, 4138, 4749, 6472, 7223
AdeI CACNNNGTG 1 cut(s) 109
AfeI AGCGCT 1 cut(s) 4609
AflII CTTAAG 2 cut(s) 6709, 7891
AflIII ACRYGT 7 cut(s) 733, 1596, 1848, 2230, 5988, 6445, 7123
AjiI CACGTC 1 cut(s) 5989
AloI GAACNNNNNNTCC 4 cut(s) 5427, 5459, 6135, 6167
Alw44I GTGCAC 3 cut(s) 2306, 2918, 8595
AlwNI CAGNNNCTG 5 cut(s) 2378, 2419, 3530, 5423, 8885
Ama87I CYCGRG 4 cut(s) 74, 6720, 7798, 7884
Aor13HI TCCGGA 1 cut(s) 835
Aor51HI AGCGCT 1 cut(s) 4609
ApaI GGGCCC 1 cut(s) 2370
ApaLI GTGCAC 3 cut(s) 2306, 2918, 8595
AseI ATTAAT 1 cut(s) 8324
Asp700I GAANNNNTTC 7 cut(s) 1773, 4327, 4961, 6200, 6697, 7739, 7953
Asp718I GGTACC 1 cut(s) 8762
AspA2I CCTAGG 1 cut(s) 8833
AspLEI GCGC 7 cut(s) 142, 2839, 3499, 3851, 4610, 4716, 7225
AsuC2I CCSGG 4 cut(s) 2112, 3411, 7885, 7886
AsuNHI GCTAGC 1 cut(s) 922
AvaI CYCGRG 4 cut(s) 74, 6720, 7798, 7884
AvrII CCTAGG 1 cut(s) 8833
AxyI CCTNAGG 3 cut(s) 399, 2402, 6086
BaeGI GKGCMC 5 cut(s) 2310, 2370, 2922, 4780, 8599
BaeI ACNNNNGTAYC 2 cut(s) 3708, 3741
BalI TGGCCA 2 cut(s) 2332, 6041
BanI GGYRCC 2 cut(s) 7222, 8762
BanII GRGCYC 6 cut(s) 81, 674, 2370, 3416, 4839, 8538
BarI GAAGNNNNNNTAC 6 cut(s) 1596, 1628, 5644, 5676, 7727, 7759
BauI CACGAG 1 cut(s) 8727
BbsI GAAGAC 5 cut(s) 617, 3099, 5991, 7685, 8704
BbvCI CCTCAGC 1 cut(s) 2371
BceAI ACGGC 2 cut(s) 34, 8868
BcgI CGANNNNNNTGC 2 cut(s) 9429, 9463
BciVI GTATCC 3 cut(s) 343, 9071, 9091
BclI TGATCA 5 cut(s) 93, 1810, 3148, 4419, 8389
BcnI CCSGG 4 cut(s) 2112, 3411, 7885, 7886
BfoI RGCGCY 3 cut(s) 143, 4611, 7226
BfrI CTTAAG 2 cut(s) 6709, 7891
BfuAI ACCTGC 3 cut(s) 710, 2056, 9171
BfuI GTATCC 3 cut(s) 343, 9071, 9091
BglI GCCNNNNNGGC 1 cut(s) 8624
BglII AGATCT 4 cut(s) 1393, 2472, 5747, 7037
BlnI CCTAGG 1 cut(s) 8833
BlpI GCTNAGC 2 cut(s) 3472, 4570
BmcAI AGTACT 4 cut(s) 1904, 3145, 5560, 8273
BmeT110I CYCGRG 4 cut(s) 74, 6720, 7798, 7884
BmgBI CACGTC 1 cut(s) 5989
BmrI ACTGGG 3 cut(s) 3455, 4840, 5363
BmtI GCTAGC 1 cut(s) 926
BmuI ACTGGG 3 cut(s) 3455, 4840, 5363
BoxI GACNNNNGTC 1 cut(s) 9045
BpiI GAAGAC 5 cut(s) 617, 3099, 5991, 7685, 8704
BplI GAGNNNNNCTC 4 cut(s) 7210, 7242, 8338, 8370
BpmI CTGGAG 6 cut(s) 1330, 2575, 4222, 5031, 5688, 7313
Bpu10I CCTNAGC 3 cut(s) 2371, 2463, 3349
Bpu1102I GCTNAGC 2 cut(s) 3472, 4570
BpuEI CTTGAG 5 cut(s) 3400, 3917, 4008, 5778, 7388
BpuMI CCSGG 4 cut(s) 2112, 3411, 7885, 7886
Bsa29I ATCGAT 2 cut(s) 8790, 9428
BsaAI YACGTR 1 cut(s) 7703
BsaBI GATNNNNATC 7 cut(s) 1648, 2032, 2429, 4521, 6396, 6501, 8304
BsaHI GRCGYC 5 cut(s) 258, 4138, 4749, 6472, 7223
BsaI GGTCTC 1 cut(s) 9237
BsaWI WCCGGW 3 cut(s) 123, 835, 4429
BsaXI ACNNNNNCTCC 6 cut(s) 5427, 5457, 5582, 5612, 8129, 8159
Bse21I CCTNAGG 3 cut(s) 399, 2402, 6086
Bse3DI GCAATG 9 cut(s) 1735, 2204, 2250, 4709, 5452, 7377, 7456, 8175, 8651
Bse8I GATNNNNATC 7 cut(s) 1648, 2032, 2429, 4521, 6396, 6501, 8304
BseAI TCCGGA 1 cut(s) 835
BseCI ATCGAT 2 cut(s) 8790, 9428
BseJI GATNNNNATC 7 cut(s) 1648, 2032, 2429, 4521, 6396, 6501, 8304
BseMI GCAATG 9 cut(s) 1735, 2204, 2250, 4709, 5452, 7377, 7456, 8175, 8651
BseRI GAGGAG 6 cut(s) 2840, 4427, 5904, 8330, 8483, 9436
BseSI GKGCMC 5 cut(s) 2310, 2370, 2922, 4780, 8599
BseX3I CGGCCG 1 cut(s) 47
BseYI CCCAGC 1 cut(s) 2085
BsgI GTGCAG 4 cut(s) 778, 1410, 4599, 8474
Bsh1236I CGCG 2 cut(s) 107, 137
Bsh1285I CGRYCG 2 cut(s) 50, 9428
BshNI GGYRCC 2 cut(s) 7222, 8762
BshVI ATCGAT 2 cut(s) 8790, 9428
BsiEI CGRYCG 2 cut(s) 50, 9428
BsiHKCI CYCGRG 4 cut(s) 74, 6720, 7798, 7884
BsiSI CCGG 8 cut(s) 22, 68, 124, 836, 2112, 3410, 4430, 7885
BsmBI CGTCTC 5 cut(s) 4756, 6161, 6938, 6956, 8706
BsmI GAATGC 4 cut(s) 236, 860, 4699, 6858
Bso31I GGTCTC 1 cut(s) 9237
BsoBI CYCGRG 4 cut(s) 74, 6720, 7798, 7884
Bsp120I GGGCCC 1 cut(s) 2366
Bsp13I TCCGGA 1 cut(s) 835
Bsp1407I TGTACA 5 cut(s) 3782, 3880, 6322, 8119, 8260
Bsp1720I GCTNAGC 2 cut(s) 3472, 4570
Bsp19I CCATGG 3 cut(s) 6036, 6528, 8956
BspDI ATCGAT 2 cut(s) 8790, 9428
BspEI TCCGGA 1 cut(s) 835
BspFNI CGCG 2 cut(s) 107, 137
BspHI TCATGA 1 cut(s) 4984
BspMAI CTGCAG 4 cut(s) 2641, 2734, 8292, 8881
BspMI ACCTGC 3 cut(s) 710, 2056, 9171
BspOI GCTAGC 1 cut(s) 926
BspQI GCTCTTC 2 cut(s) 57, 4149
BspT107I GGYRCC 2 cut(s) 7222, 8762
BspTI CTTAAG 2 cut(s) 6709, 7891
BspTNI GGTCTC 1 cut(s) 9237
BsrBI CCGCTC 2 cut(s) 12, 38
BsrDI GCAATG 9 cut(s) 1735, 2204, 2250, 4709, 5452, 7377, 7456, 8175, 8651
BsrGI TGTACA 5 cut(s) 3782, 3880, 6322, 8119, 8260
BssNAI GTATAC 1 cut(s) 8037
BssNI GRCGYC 5 cut(s) 258, 4138, 4749, 6472, 7223
BssSI CACGAG 1 cut(s) 8727
BssT1I CCWWGG 6 cut(s) 5227, 6036, 6528, 8833, 8956, 9267
Bst1107I GTATAC 1 cut(s) 8037
Bst2BI CACGAG 1 cut(s) 8727
BstACI GRCGYC 5 cut(s) 258, 4138, 4749, 6472, 7223
BstAFI CTTAAG 2 cut(s) 6709, 7891
BstAPI GCANNNNNTGC 1 cut(s) 3004
BstAUI TGTACA 5 cut(s) 3782, 3880, 6322, 8119, 8260
BstBAI YACGTR 1 cut(s) 7703
BstDSI CCRYGG 5 cut(s) 50, 135, 6036, 6528, 8956
BstEII GGTNACC 1 cut(s) 8828
BstENI CCTNNNNNAGG 1 cut(s) 3980
BstFNI CGCG 2 cut(s) 107, 137
BstH2I RGCGCY 3 cut(s) 143, 4611, 7226
BstHHI GCGC 7 cut(s) 142, 2839, 3499, 3851, 4610, 4716, 7225
BstMCI CGRYCG 2 cut(s) 50, 9428
BstPAI GACNNNNGTC 1 cut(s) 9045
BstPI GGTNACC 1 cut(s) 8828
BstSLI GKGCMC 5 cut(s) 2310, 2370, 2922, 4780, 8599
BstUI CGCG 2 cut(s) 107, 137
BstV2I GAAGAC 5 cut(s) 617, 3099, 5991, 7685, 8704
BstX2I RGATCY 9 cut(s) 598, 1393, 1643, 2472, 4733, 5747, 7025, 7037, 8400
BstYI RGATCY 9 cut(s) 598, 1393, 1643, 2472, 4733, 5747, 7025, 7037, 8400
BstZ17I GTATAC 1 cut(s) 8037
BstZI CGGCCG 1 cut(s) 47
Bsu15I ATCGAT 2 cut(s) 8790, 9428
Bsu36I CCTNAGG 3 cut(s) 399, 2402, 6086
BsuI GTATCC 3 cut(s) 343, 9071, 9091
BsuTUI ATCGAT 2 cut(s) 8790, 9428
BtgI CCRYGG 5 cut(s) 50, 135, 6036, 6528, 8956
BtgZI GCGATG 4 cut(s) 3631, 9104, 9370, 9429
BtrI CACGTC 1 cut(s) 5989
BtsI GCAGTG 5 cut(s) 697, 2869, 5245, 6234, 6622
BveI ACCTGC 3 cut(s) 710, 2056, 9171
CaiI CAGNNNCTG 5 cut(s) 2378, 2419, 3530, 5423, 8885
CciI TCATGA 1 cut(s) 4984
CfoI GCGC 7 cut(s) 142, 2839, 3499, 3851, 4610, 4716, 7225
Cfr42I CCGCGG 1 cut(s) 138
Cfr9I CCCGGG 1 cut(s) 7884
ClaI ATCGAT 2 cut(s) 8790, 9428
CseI GACGC 5 cut(s) 247, 4065, 6474, 6920, 6973
CspCI CAANNNNNGTGG 2 cut(s) 7650, 7685
DinI GGCGCC 1 cut(s) 7224
DraI TTTAAA 4 cut(s) 303, 1936, 4299, 8304
DraIII CACNNNGTG 1 cut(s) 109
DrdI GACNNNNNNGTC 5 cut(s) 3744, 5739, 8479, 8857, 9546
DseDI GACNNNNNNGTC 5 cut(s) 3744, 5739, 8479, 8857, 9546
EaeI YGGCCR 4 cut(s) 33, 47, 2330, 6039
EagI CGGCCG 1 cut(s) 47
EciI GGCGGA 1 cut(s) 40
Ecl136II GAGCTC 3 cut(s) 79, 672, 8536
EclXI CGGCCG 1 cut(s) 47
Eco130I CCWWGG 6 cut(s) 5227, 6036, 6528, 8833, 8956, 9267
Eco147I AGGCCT 1 cut(s) 636
Eco24I GRGCYC 6 cut(s) 81, 674, 2370, 3416, 4839, 8538
Eco31I GGTCTC 1 cut(s) 9237
Eco32I GATATC 3 cut(s) 1620, 2431, 4677
Eco47III AGCGCT 1 cut(s) 4609
Eco52I CGGCCG 1 cut(s) 47
Eco53kI GAGCTC 3 cut(s) 79, 672, 8536
Eco81I CCTNAGG 3 cut(s) 399, 2402, 6086
Eco88I CYCGRG 4 cut(s) 74, 6720, 7798, 7884
Eco91I GGTNACC 1 cut(s) 8828
EcoICRI GAGCTC 3 cut(s) 79, 672, 8536
EcoNI CCTNNNNNAGG 1 cut(s) 3980
EcoO109I RGGNCCY 9 cut(s) 2366, 2367, 2886, 3985, 4860, 6082, 6819, 8481, 8506
EcoO65I GGTNACC 1 cut(s) 8828
EcoRI GAATTC 2 cut(s) 4648, 7460
EcoRV GATATC 3 cut(s) 1620, 2431, 4677
EcoT14I CCWWGG 6 cut(s) 5227, 6036, 6528, 8833, 8956, 9267
EcoT22I ATGCAT 3 cut(s) 6285, 6958, 7379
EcoT38I GRGCYC 6 cut(s) 81, 674, 2370, 3416, 4839, 8538
EgeI GGCGCC 1 cut(s) 7224
EheI GGCGCC 1 cut(s) 7224
ErhI CCWWGG 6 cut(s) 5227, 6036, 6528, 8833, 8956, 9267
Esp3I CGTCTC 5 cut(s) 4756, 6161, 6938, 6956, 8706
FalI AAGNNNNNCTT 4 cut(s) 4553, 4585, 6188, 6220
FauI CCCGC 1 cut(s) 4567
FauNDI CATATG 3 cut(s) 4530, 6025, 8092
FbaI TGATCA 5 cut(s) 93, 1810, 3148, 4419, 8389
FblI GTMKAC 6 cut(s) 792, 4079, 5660, 6276, 8036, 8179
FriOI GRGCYC 6 cut(s) 81, 674, 2370, 3416, 4839, 8538
GlaI GCGC 7 cut(s) 141, 2838, 3498, 3850, 4609, 4715, 7224
GsaI CCCAGC 1 cut(s) 2089
GsuI CTGGAG 6 cut(s) 1330, 2575, 4222, 5031, 5688, 7313
HaeII RGCGCY 3 cut(s) 143, 4611, 7226
HapII CCGG 8 cut(s) 22, 68, 124, 836, 2112, 3410, 4430, 7885
HgaI GACGC 5 cut(s) 247, 4065, 6474, 6920, 6973
HhaI GCGC 7 cut(s) 142, 2839, 3499, 3851, 4610, 4716, 7225
Hin1I GRCGYC 5 cut(s) 258, 4138, 4749, 6472, 7223
Hin6I GCGC 7 cut(s) 140, 2837, 3497, 3849, 4608, 4714, 7223
HinP1I GCGC 7 cut(s) 140, 2837, 3497, 3849, 4608, 4714, 7223
HincII GTYRAC 7 cut(s) 725, 4747, 5869, 6277, 6897, 7207, 8244
HindII GTYRAC 7 cut(s) 725, 4747, 5869, 6277, 6897, 7207, 8244
HindIII AAGCTT 9 cut(s) 338, 380, 431, 3816, 3956, 5075, 5398, 7049, 9035
HpaII CCGG 8 cut(s) 22, 68, 124, 836, 2112, 3410, 4430, 7885
Hpy99I CGWCG 3 cut(s) 161, 4731, 5970
Hsp92I GRCGYC 5 cut(s) 258, 4138, 4749, 6472, 7223
HspAI GCGC 7 cut(s) 140, 2837, 3497, 3849, 4608, 4714, 7223
KasI GGCGCC 1 cut(s) 7222
Kpn2I TCCGGA 1 cut(s) 835
KpnI GGTACC 1 cut(s) 8766
Ksp22I TGATCA 5 cut(s) 93, 1810, 3148, 4419, 8389
KspI CCGCGG 1 cut(s) 138
LguI GCTCTTC 2 cut(s) 57, 4149
MbiI CCGCTC 2 cut(s) 12, 38
MfeI CAATTG 4 cut(s) 243, 4847, 5575, 7421
MflI RGATCY 9 cut(s) 598, 1393, 1643, 2472, 4733, 5747, 7025, 7037, 8400
MlsI TGGCCA 2 cut(s) 2332, 6041
MluNI TGGCCA 2 cut(s) 2332, 6041
Mly113I GGCGCC 1 cut(s) 7223
MmeI TCCRAC 6 cut(s) 1836, 3906, 4294, 5755, 7904, 9075
Mox20I TGGCCA 2 cut(s) 2332, 6041
Mph1103I ATGCAT 3 cut(s) 6285, 6958, 7379
MroI TCCGGA 1 cut(s) 835
MroXI GAANNNNTTC 7 cut(s) 1773, 4327, 4961, 6200, 6697, 7739, 7953
MscI TGGCCA 2 cut(s) 2332, 6041
MslI CAYNNNNRTG 6 cut(s) 1183, 1479, 4098, 4994, 6041, 7841
Msp20I TGGCCA 2 cut(s) 2332, 6041
MspA1I CMGCKG 5 cut(s) 137, 983, 1166, 4790, 7234
MspCI CTTAAG 2 cut(s) 6709, 7891
MspI CCGG 8 cut(s) 22, 68, 124, 836, 2112, 3410, 4430, 7885
MunI CAATTG 4 cut(s) 243, 4847, 5575, 7421
Mva1269I GAATGC 4 cut(s) 236, 860, 4699, 6858
MvnI CGCG 2 cut(s) 107, 137
NarI GGCGCC 1 cut(s) 7223
NciI CCSGG 4 cut(s) 2112, 3411, 7885, 7886
NcoI CCATGG 3 cut(s) 6036, 6528, 8956
NdeI CATATG 3 cut(s) 4530, 6025, 8092
NheI GCTAGC 1 cut(s) 922
NmeAIII GCCGAG 1 cut(s) 132
NsiI ATGCAT 3 cut(s) 6285, 6958, 7379
PaeI GCATGC 2 cut(s) 419, 4688
PaeR7I CTCGAG 2 cut(s) 74, 6720
PagI TCATGA 1 cut(s) 4984
PceI AGGCCT 1 cut(s) 636
PciI ACATGT 5 cut(s) 1596, 1848, 2230, 6445, 7123
PciSI GCTCTTC 2 cut(s) 57, 4149
PcsI WCGNNNNNNNCGW 4 cut(s) 2289, 5965, 9286, 9547
PctI GAATGC 4 cut(s) 236, 860, 4699, 6858
PdmI GAANNNNTTC 7 cut(s) 1773, 4327, 4961, 6200, 6697, 7739, 7953
PflMI CCANNNNNTGG 3 cut(s) 2419, 2554, 6906
PfoI TCCNGGA 1 cut(s) 4141
Ple19I CGATCG 1 cut(s) 9428
PluTI GGCGCC 1 cut(s) 7226
Ppu21I YACGTR 1 cut(s) 7703
PpuMI RGGWCCY 5 cut(s) 2886, 3985, 4860, 8481, 8506
PscI ACATGT 5 cut(s) 1596, 1848, 2230, 6445, 7123
PshAI GACNNNNGTC 1 cut(s) 9045
PshBI ATTAAT 1 cut(s) 8324
PsiI TTATAA 3 cut(s) 5300, 8567, 9185
Psp124BI GAGCTC 3 cut(s) 81, 674, 8538
Psp1406I AACGTT 1 cut(s) 3918
Psp5II RGGWCCY 5 cut(s) 2886, 3985, 4860, 8481, 8506
PspEI GGTNACC 1 cut(s) 8828
PspFI CCCAGC 1 cut(s) 2085
PspOMI GGGCCC 1 cut(s) 2366
PspPPI RGGWCCY 5 cut(s) 2886, 3985, 4860, 8481, 8506
PspXI VCTCGAGB 1 cut(s) 74
PstI CTGCAG 4 cut(s) 2641, 2734, 8292, 8881
PstNI CAGNNNCTG 5 cut(s) 2378, 2419, 3530, 5423, 8885
PsuI RGATCY 9 cut(s) 598, 1393, 1643, 2472, 4733, 5747, 7025, 7037, 8400
PvuI CGATCG 1 cut(s) 9428
PvuII CAGCTG 3 cut(s) 983, 1166, 7234
RseI CAYNNNNRTG 6 cut(s) 1183, 1479, 4098, 4994, 6041, 7841
SacI GAGCTC 3 cut(s) 81, 674, 8538
SacII CCGCGG 1 cut(s) 138
SalI GTCGAC 1 cut(s) 6275
SapI GCTCTTC 2 cut(s) 57, 4149
ScaI AGTACT 4 cut(s) 1904, 3145, 5560, 8273
SfoI GGCGCC 1 cut(s) 7224
Sfr274I CTCGAG 2 cut(s) 74, 6720
Sfr303I CCGCGG 1 cut(s) 138
SgrBI CCGCGG 1 cut(s) 138
SlaI CTCGAG 2 cut(s) 74, 6720
SmaI CCCGGG 1 cut(s) 7886
SmiI ATTTAAAT 1 cut(s) 8304
SmiMI CAYNNNNRTG 6 cut(s) 1183, 1479, 4098, 4994, 6041, 7841
SmlI CTYRAG 9 cut(s) 74, 3415, 3896, 4023, 5793, 6709, 6720, 7403, 7891
SmoI CTYRAG 9 cut(s) 74, 3415, 3896, 4023, 5793, 6709, 6720, 7403, 7891
SphI GCATGC 2 cut(s) 419, 4688
SseBI AGGCCT 1 cut(s) 636
SspDI GGCGCC 1 cut(s) 7222
SstI GAGCTC 3 cut(s) 81, 674, 8538
StuI AGGCCT 1 cut(s) 636
StyI CCWWGG 6 cut(s) 5227, 6036, 6528, 8833, 8956, 9267
SwaI ATTTAAAT 1 cut(s) 8304
TaqII GACCGA 2 cut(s) 764, 8071
TauI GCSGC 7 cut(s) 15, 38, 49, 140, 2528, 6749, 8029
TspGWI ACGGA 4 cut(s) 1407, 3332, 6452, 7774
TspMI CCCGGG 1 cut(s) 7884
Van91I CCANNNNNTGG 3 cut(s) 2419, 2554, 6906
Vha464I CTTAAG 2 cut(s) 6709, 7891
VneI GTGCAC 3 cut(s) 2306, 2918, 8595
VspI ATTAAT 1 cut(s) 8324
XagI CCTNNNNNAGG 1 cut(s) 3980
XhoI CTCGAG 2 cut(s) 74, 6720
XmaI CCCGGG 1 cut(s) 7884
XmaJI CCTAGG 1 cut(s) 8833
XmiI GTMKAC 6 cut(s) 792, 4079, 5660, 6276, 8036, 8179
XmnI GAANNNNTTC 7 cut(s) 1773, 4327, 4961, 6200, 6697, 7739, 7953
ZraI GACGTC 3 cut(s) 4139, 4750, 6473
ZrmI AGTACT 4 cut(s) 1904, 3145, 5560, 8273
Zsp2I ATGCAT 3 cut(s) 6285, 6958, 7379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.