Rorug02G0114000

regulation of parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004207
Physical Location & Seq
Forward (+)
9740159 .. 9740784
626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0114000.1

Sequence Viewer

Length: 465 bp
ATGCTCGGAGCAAGGAAGTTTGGGATTATCAGCGTCTCACAAATTGGGTGCTGCCCTGCCCAGCGAAAGCTCAGTGAAAGAGGCGGGTGTTTGGAGGCCATGAATGCTTATGCACACTTGTTCTATACGTCACTCCAAGTCCTCTTGCAGAAGTTGAGCTTACAATGCAGAGGGATGATCTACGCACTTGGAGATACATATAAAATGACCCAAGTTATTATGGACAATCCTCTTCCATTTGGGTTGGTGGAGGTGAATAAGGCTTGTTGTGGTAAAGGGAAGTTGAATGCAGAGTTTCCATGTGAACCAGATTCGGATTTGTGTGAAAATCGCCGTAGCTACTTGTTCTGGGATCAATACCATTCAACAGAGGCTGCGTCTGGAATCCTAGCCATATATCTGTTTGGTGGTGCTCCGGTTTTGGTGACACCCATTAGTTTCGGTCAATTGGCCCTCCTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHR-BD VPS13_C

Protein Analysis

154

Amino Acids

16.87

Weight (kDa)

7.51

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 3 - 131 9.7e-08 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 84
AclWI GGATC 1 cut(s) 360
AgsI TTSAA 2 cut(s) 286, 366
AluBI AGCT 3 cut(s) 70, 159, 339
AluI AGCT 3 cut(s) 70, 159, 339
Alw21I GWGCWC 1 cut(s) 415
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 1 cut(s) 360
AlwNI CAGNNNCTG 1 cut(s) 374
AoxI GGCC 2 cut(s) 96, 450
ApeKI GCWGC 2 cut(s) 51, 374
AspS9I GGNCC 1 cut(s) 451
AsuHPI GGTGA 2 cut(s) 265, 436
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 2 cut(s) 38, 361
BceAI ACGGC 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 1 cut(s) 389
BisI GCNGC 2 cut(s) 52, 375
BlsI GCNGC 2 cut(s) 53, 376
BmgT120I GGNCC 1 cut(s) 451
BsaWI WCCGGW 1 cut(s) 415
BseGI GGATG 1 cut(s) 180
BseMII CTCAG 1 cut(s) 85
BseXI GCAGC 2 cut(s) 38, 361
BseYI CCCAGC 1 cut(s) 60
BshFI GGCC 2 cut(s) 98, 452
BsiHKAI GWGCWC 1 cut(s) 415
BsiSI CCGG 1 cut(s) 416
BsmAI GTCTC 1 cut(s) 40
BsmBI CGTCTC 1 cut(s) 40
BsmI GAATGC 2 cut(s) 109, 292
BsnI GGCC 2 cut(s) 98, 452
Bsp1286I GDGCHC 1 cut(s) 415
Bsp143I GATC 2 cut(s) 177, 352
BspACI CCGC 1 cut(s) 84
BspANI GGCC 2 cut(s) 98, 452
BspCNI CTCAG 1 cut(s) 84
BspPI GGATC 1 cut(s) 360
BssMI GATC 2 cut(s) 177, 352
Bst6I CTCTTC 1 cut(s) 237
BstDEI CTNAG 1 cut(s) 71
BstF5I GGATG 1 cut(s) 180
BstKTI GATC 2 cut(s) 180, 355
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 2 cut(s) 177, 352
BstMWI GCNNNNNNNGC 3 cut(s) 104, 165, 458
BstV1I GCAGC 2 cut(s) 38, 361
BsuRI GGCC 2 cut(s) 98, 452
BtsCI GGATG 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 79
CaiI CAGNNNCTG 1 cut(s) 374
Cfr13I GGNCC 1 cut(s) 451
CseI GACGC 2 cut(s) 22, 366
CviAII CATG 2 cut(s) 100, 300
CviJI RGCY 8 cut(s) 70, 98, 159, 263, 339, 374, 392, 452
CviKI_1 RGCY 8 cut(s) 70, 98, 159, 263, 339, 374, 392, 452
DdeI CTNAG 1 cut(s) 71
DpnI GATC 2 cut(s) 179, 354
DpnII GATC 2 cut(s) 177, 352
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Esp3I CGTCTC 1 cut(s) 40
FaeI CATG 2 cut(s) 103, 303
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FatI CATG 2 cut(s) 99, 299
FauI CCCGC 1 cut(s) 77
Fnu4HI GCNGC 2 cut(s) 52, 375
FokI GGATG 1 cut(s) 187
Fsp4HI GCNGC 2 cut(s) 52, 375
FspBI CTAG 1 cut(s) 389
GluI GCNGC 2 cut(s) 52, 375
GsaI CCCAGC 1 cut(s) 64
HaeIII GGCC 2 cut(s) 98, 452
HapII CCGG 1 cut(s) 416
HgaI GACGC 2 cut(s) 22, 366
Hin1II CATG 2 cut(s) 103, 303
HinfI GANTC 2 cut(s) 311, 384
HpaII CCGG 1 cut(s) 416
HphI GGTGA 2 cut(s) 265, 436
Hpy166II GTNNAC 1 cut(s) 305
Hpy188I TCNGA 2 cut(s) 8, 316
Hpy188III TCNNGA 1 cut(s) 381
Hpy8I GTNNAC 1 cut(s) 305
HpyCH4IV ACGT 1 cut(s) 128
HpyCH4V TGCA 5 cut(s) 113, 148, 168, 290, 461
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 165, 458
HpyF3I CTNAG 1 cut(s) 71
HpySE526I ACGT 1 cut(s) 128
Hsp92II CATG 2 cut(s) 103, 303
Kzo9I GATC 2 cut(s) 177, 352
LmnI GCTCC 2 cut(s) 8, 418
LpnPI CCDG 6 cut(s) 69, 74, 321, 334, 366, 429
Lsp1109I GCAGC 2 cut(s) 38, 361
MaeI CTAG 1 cut(s) 389
MaeII ACGT 1 cut(s) 128
MaeIII GTNAC 2 cut(s) 129, 424
MalI GATC 2 cut(s) 179, 354
MboI GATC 2 cut(s) 177, 352
MboII GAAGA 1 cut(s) 224
MfeI CAATTG 1 cut(s) 446
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 2 cut(s) 42, 446
MnlI CCTC 8 cut(s) 74, 88, 152, 164, 240, 244, 364, 464
MspI CCGG 1 cut(s) 416
MunI CAATTG 1 cut(s) 446
Mva1269I GAATGC 2 cut(s) 109, 292
MwoI GCNNNNNNNGC 3 cut(s) 104, 165, 458
NdeII GATC 2 cut(s) 177, 352
NlaIII CATG 2 cut(s) 103, 303
NmuCI GTSAC 2 cut(s) 129, 424
PctI GAATGC 2 cut(s) 109, 292
PfeI GAWTC 2 cut(s) 311, 384
PkrI GCNGC 2 cut(s) 53, 376
PspFI CCCAGC 1 cut(s) 60
PspPI GGNCC 1 cut(s) 451
PstNI CAGNNNCTG 1 cut(s) 374
SatI GCNGC 2 cut(s) 52, 375
Sau3AI GATC 2 cut(s) 177, 352
Sau96I GGNCC 1 cut(s) 451
SduI GDGCHC 1 cut(s) 415
SetI ASST 5 cut(s) 72, 131, 161, 255, 341
Sse9I AATT 2 cut(s) 42, 446
SsiI CCGC 1 cut(s) 84
SspMI CTAG 1 cut(s) 389
TaiI ACGT 1 cut(s) 131
TaqII GACCGA 1 cut(s) 431
TasI AATT 2 cut(s) 42, 446
TfiI GAWTC 2 cut(s) 311, 384
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 2 cut(s) 129, 424
TseI GCWGC 2 cut(s) 51, 374
Tsp45I GTSAC 2 cut(s) 129, 424
TspDTI ATGAA 1 cut(s) 116
TspRI CASTG 1 cut(s) 79
XspI CTAG 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.