MD02G1159300.v1.1

regulation of parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004207
Physical Location & Seq
Reverse (-)
13361712 .. 13381990
20279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1159300.v1.1.491

Sequence Viewer

Length: 9465 bp
ATGTTCCCGAGCACCGCAGCGCGACGGAGACTGGCTTCTGTGCTGCGGCCATGGCTCCGGGAGGAGCCCGACCTCGATCTCAAATTAGGGGTTATCAATTCCCACGCGGTCGCCAGGAACCTCCGGTTCGACACTGGGGCTCTCAACGAGCTCTTCGACGAATCGGCCGAGTTTTCCTTCTCAGAGGTCACTGTCGAACACTTGAGCGTTCGGTTCTCGAACTGGCTCGTCCCTGCCTTCTCAATCGAATTTCATGGCGTCAATGTGGAACTAGCAGCTCGGGAGGAGAAAAATTTAAAGCGAAAACCGAGGGAGGAATATGCAGAAGATGTGAAGAAGAAGAAGCTTTCGGAGATAGATCCCGAGGGTAGTGCTTTACATGGTGTTTTTGAAAAGCTTGTGGCCACTTCTACTCCAACAAAAAGTTTTAAAGACACCCTTTGGAATCTCTTACTGAAACACTGTCAGCTTCGAGTGCACGATATCAATGTACAAGTTAAGGTTCCCATCTTAAATGATTCACTTGTTTGTGTGTTCGACGTAAGAGATATCAATGCAGATCCTCAGTATCTTGATCACAGGTGTCTTCTTCGAGGTCTATTTGGTGCACTCTTTCTACCTTTGAAAGAGAGCTCTTTTGTTATAGCAGGCAGTAGTTTTGAGGTGGGGTTCAAGAGAGAAAATCAGCTCAAAAACGTGTTGTTATTGACTGATTTGTGCACTTGCATTAGATTAAATAATTTTCAGCTCGTGGATGTAAATCTTCAATTTCCAGAATTACATTGTACATTTTCTCCGGATGACATTTATTGGTATTCAGCATTGGGTAAATTGTCATCCCAAGAATCTCGATCTGCTAGAAATGGTGTCCAACTATGGAAACTAGCTGCAAGAAGTATGGACAACTTGACTTCAGGTTCTAGGTGGTCGTTGCAAAAATTAGTTGGTCTTGTTTGTTTATGGCTACGTTATGCTAATGCTTACGAGCAGTTATTGTTAGCAATTGGATATTCTGATGATCACTCATTGAAGAGATCAGCTACTAGAATTTCTCAGGACAAGATGTTTTTGGCTTCTGTAAAGAACCAATGCAAGGTGATTTTTGATATAGAGAAACAGTTGCCGGCTGAAGCCATTGTACAGGCATGGAGAATCGCCCGTCATAGAGCAGCATCAAATGCTCAATCTACCAAAGATAGTTTAAAAAAATCCTCCATGATTACTCACTTCAACTTCCCTCTCAAGATCCTTCTGGTACTAGCCTGTACCTGGAGAGTTCTGTGCAAGATATTCCACTTTATCATACACTTGTTAACTTTTAGGAAAGTATTGGACAAAGAGCCAGACAATGCAAATTTGGATATTGTTTCTGGAGGTCTGTGTAAAGATTTTTGCTTCATCGTGATCTTTGGAAATGTTCTAATCACTATTTCTCATATAAATGAAGTTCAAATCTCAGTAAATAAACAATTAGAATCACATATTGGAATCTCTTGTTCAGATTTCCTATCATTTCGTCTTTCAGTTGATTCGCTGTTCTTAAAATATGTAGAAGACATGTGTGAACAGGCTGTACTTATATCATGTGGGCAGTTGAAGGTTAGATCCTCATCTCTTTCGAAAGCTTCTGTTAGAGAAATCATTTCAAATGTGGAAGAACATTGGAAGGAGAGAAATGATGATTTCAAGAATATTTTGTGGGGTGAGCCAGCACAAACTTTTCCCCTTTCAGAAACTTACGAAACTGCTTATGCTGATCATGCGGAAGGTGCTTGTGCCTCATTTTTGAAAACATTTCTTGGAGACATGTGGTTGAACTGGAAAAGATCCAGCAAAAAATTTGAAAAAAGTGAGATTCGGTACTTTGAAAACCCATTCCTTCTCTGTGAAATTAAAAACTGGACATATCCAGACCTCAAAAACTCGGAGTCTGGGTTTTCGAAGTTGTTTTTGACCATGGGAAAGTTGAATCTAGTTTTGGGATGTCCCTCCATACTATCAGTATCTCTGCTCTTAAAGCAAATGCAACATGCTCTATGCTGGACGGAGGACAATGGTCAGTCAGGGGTTCTGCCACATTCTCCAAGAGCTAGTAATGATAGCATTTGCACGCACTATGCTAATAGATTGGAAATGGCATTGCTTAAGACGCTTCCAGAGAAACATGTTCAACTAGGTATATTTGCAGCTGGTCCTCACATCCGTATGTCATTGGGAAAGAGTTTTGATGGTGGCAGCAAAGACATAAATCATCAAGTGAGTCAGGAGGATTTTCACCTTGCATTTGATGTGCACAGCATAGAGGCTGCAGTATGGCCAACCTCACAATTTGATCTGGCATCATTTGTTGCACCCTTAGGAGCTGATGATGTAGAAACAAAGTGCCTCAGGTTGGAGAAGCCTCTTGGAGTTGATAAATTTAAATCAGATAATGGAAAGTATCAATCTCAGGGGTCAGTTTCTCTTGGTTTTTATTTACACGTTGATGGTTTAGATGCTTACCTGATCGACTCAGCAGGAAAAAAACAAAGTGACATTTTAGTTCTGAAGCCAATGACCGTGTGGATGTCATCCTTAAGGGAGTATGCTCATTCATTTAGCACAAATTTTGTTGCTCTCTCAGCAGCTTTCTATGGGACAGCTGAAGGATTCACTATTTTGTCATATACAGATGAGATTTATGTTTTCCTTCAGGTGCTTGCCAATTTATCTTCTGCAATGTCATATTCATTTAGCAGATTTGCAGCTCTAAGTTACTCGCCTTTCAAGTTTGCAATACAAGAATTTGCTTTTCCTGAATCTGAGAATGTGGAGACAACTGTACATGGAGCTCCCTTCAATTATAGCAGTTTCCTAGTTTCATTTAATGGGTCCTTCAAACTCAAGTCTATCGATATCATTCTTCACAAGTCAAGGATCAGTGGTAATCTGAAGAGTTGTATAGGAACCTCTGATGTTTCAAGTAGCAAAAAGTTAGCTGATCATGACTTGCCTGACTGTGGCATTTGGATATCAATTCACCAAACAACTGCTGATATATCTTCAACAGAAGGAAAAGGGAAAATTATTTCTTATTTATCAGAAATCCAGTCAATAATTTTTAGATATAAGAATCAAAAAGGAGAAAGTATGGATCATTCTGCACGTGGTGATCTACTGCAATCTCTTGATTGCTTATATGAACTCTCACTTTCTAGTTGTGTATTTCATTTATTGCTGCCTCTCTCTCAGAATAACCCATCTTCAGGTGGTGTTAGCAATGCACTTGGTACCTCAACTTCTGTTGGTGAAATAGTGCACGTGGAAAATCTTCCATTCACCACTGATTCTGAAAGCCCAAATGGTCAGAACTGTATCTTTCTTCAGGAGACAGAATTTGCTTCAAATATTCCACCACCTGATGCAAGTCATTGGTTACTGATAAATGTAACATTGGGTTATATTTATATGGGAAGGTACTCGACAAAGAATGTTATGAATGGATATCAATTACACAAGTTTGTGTCTTCAGTTTATGTTGGTGGTGAATTTCAGACAATCTGTTGCGGAATTCAGGGTGGTTTTCTGTTCCTTGAAATAAAAGCTTTGGCTACCTTCATCAACTGTTTTGCTTCATATCGTCTTTGTTTCGCAAATCTTGTATCTGGTTTACGATTGTCTGATGAACACATTGAAGAAGCTGCAGTTTCTGTGGATATGACCAGACAAAATAATCATTGTATCGACGAATATATGCAAGAGGCTCACTGTACATCTCCACATGCCAAGAACACACAAATGGAAGCTTTCATCTTAAATCTATCTCAGTTTTCTTGTGCTCTTGTGATTGAAGATGAAAATGGTGGTATGCAGGAAATTGTTCTTGAGGTTGATGTCCAGTTTAAGTTTCAACTGGAAAACATGAGAAGAAAACTTATATTTGATCTTTCTCGCTTGTCAATACTTTCTAGGGTCTTTCAAGAAATTGTGGAAAATGAAATCCAAATTTCTCAATTTTCTGTTAATGATGCATGTTCTTCAAGAGATCCTGGTCCAGAGGATGAGTATTCTGTACCTAATTCCCTGCCTGAGACCTTCCGTCTAAGTCATATCGTGAAGCATGCAGGTGCTCTCATGTCAGTTGAGATGCCTTTAAGTTATCCTTTGTGCTTGAATGAGGTTTGGGTTGGAAGTGGATCTATTTCAGGTTTCGATATCACAATATCCCTATCTCAAATACAGGTGCTTTTGTCTATGATGTCATCCTTCTCTGAGGTAACCAAAAAAGAGATGGTTGGTGAATCGGATAGAAGGCACATGTCCAGTGGCGAGGAATTCAAAAACAGTATGGAGACATTGGTTCCGAACGGTGCAATAGTGGCAATTCAAGATGTCCATCAGCACATGTATTTTACCGTTGAGGGGGAGGAGAATAAGTACAGTGTGGCGGGTGCAGTTCATTATTCTCTTGTTGGGGAAAGGGCTCTTTTCAGGGTCAAGTATCAAAATCAAGGGAGATGGAAGTCATCAGTTTCATGGTTCTCCTTGATATCATTGTACGCTAAGAATGGCACAGGGGAGCCATTGCGCTTGAATTACCGTCCAGGATCTGGTTTTGTTGACTTATCTCGCGCTAATGATAATAGGTGGGCACTTTGGAGAGCACTTTCTTGTGAGCCTGAAAATACTGATGGTGACATTGACTGGGAGCCCAACAATCAATTGGTTCAAAAGACATTTTACCTCTTGAACAAGAAGAATGACTCTGCTGTTGCTTTCGTAGATGGGATTCCAGAGTTTGTTCCGAAGCCTGGAAATCCATTCAAGTTGAAAGTGTTCCATAATGTTTCTGTAGCTTGTGACGTAAAGATGGATGGTTACCTTGGGGAGGCATCTGTAACTGGTCCGCAACATGATGCACTCAGGGATGACGGAAAAACTTCTGCAAGAAGTGGAAAGCTTCCTTGCATTGATATTACCTTTGACAATATTTCTTTGACCGTTTTCCATGAACTTGTAGATACAAAAAACATGTTTCCTCTCCTTCGTGGTTGCATTGACAGAACCAAACTTACTGTCCAAATTCTCCCTTCTAAAACCAGGGTGATAAGCACGTCAACTGCACTTCTAGATTACTTTGATGCACAAAGAAACTTATGGAGAGAGCTTCTCCACCCAGTTGAAATATGCATCTTCTATCGTTCTAGCGTCCAGCTTCAGGGATCTGAAGCTGTTTCACGAGTACCTGTTCATATTCACTGCAGAACTAAAGAGTTGAATGTATCCCTATCCGAGCTTTCAATGGACATACTTCTCTTTGTAATCGGGAAATTACATTTAGCTGGTCCTTATTCAGTGAAAAGTACTAAGATTTGGGCCAATTGCTGCAAGGTGGTGAACCAGTCAGGATTGAACCTTCTATGTCACTTCTTTGACAAGCAAAGTGTAACAGTTTCCAAAAGGCAGTCTGCATCCATTATTTTAAGGTGCTCTGATTTGGAAAACAAACCTTCAGAAATTGCGTCAGTTGCATCAATCCAACTCACTGATCCTAGTAGATCTTTTATGACTAAATCAATTGAGGTCTCTCTTTTAGATGCTCGAGTACTTGCTTCGAGAACACGCATAACATCACTTCAGGATTCAAGAACTTACCCTGGGCCATTTGTTGTTGTTGATGTTTCAAGGAAGTCCGAGGATGGGTTGTCAATTGTTATTTCTCCTCTGACAAGAATACATAATGAAACCGGACTGCCTATAAAGCTACGATTTCGACGAGCTCAGCAGAAGGAAGATGAGTTTGCTTCTGAGGTGCTAAATGCGGGTGATACAGTTGATGACTCCATGGCCATGTTTGATGGTATAAATCTGTCTGGTGGAGAAAAAAAGGCACTGATGTCTTTAGCTGTTGGTGACTTCTTGTTGTCTTTTAGACCTGAAATTTCAGATGGGTTAATGAATTCCAAGAATCCACTCATAGTGGAATGGTCAGATGATCTTAAAGGTGGAAAGGCAGTCCGTCTCTCTGGAATTTTTGATAGATTAGGTTACAGAGTGCGGAATGCTTTGTTTACTGAATCGGTGAAATGTTCCTTCAGCACTGCATATTGTATCCTTAAATCTGACGGAGCCAGTATTTCCAACATGCATTTTCTAGTTCAGAGTATTGGGAGAAGTGTTCCTGTTGTAGAACCTAATAGGTCTACGGATGGTCTTGAAAATAGCAAATTACCAGTTGCACTGCAAGAGCAAAAGGATATTTATCTTCTTCCTACTGTACGTGTATCCAATTTGTTGCACACCGAGATACATGTTTTCTTAAGTGAATCAGATCGATGTTCCACTACTGGGTCTGACAACAACAGAAATCAGTCAACTGTGTCATGTGGTTCGATGGTTGAATTTTATGCGAATCCATCCATAATTTACTTTACCGTTACTTTGACTGCTTATAATACAAGCTGCAAACCAGTGAACAGTAGTGATTGGGTAAAAAAGTTGCTGAAGCAGAAGAGTGATGTCCCTTGTTTAGACATTGATCTGGAATTTGGTGGGGGAAAACATTTTGCTTCCTTAAGATTGTCTCGAGGGAACAGGGGCACGCTAGAGGCTGCTATTTTCACGTCATATTCCCTGAGAAATGATACAGAGTTTACCCTTTATTTCTTTGCCCCTAATAAGAAGCCTCTTTCTAGGGATGAAGTTGAGAACTATGGTTCTGGCATTCCTCCAGAGGTGGGATTATATTTGCCTCCAAAGTCAATTGGATCATGGTTTTTACGACCCAACAAAGTGTCTCTCAAATTTCTGGAAGATAATGCATCAGAGACACTGATCGACTTGGATGCTTTATCGGGCCTTGCGGAAGTAAGCTTGGAAATGGAGGACGGATCTGGAGTCAAATATATTACAAAGCTTGGGGTTTCCACGGGACCACCTTTGAGTAAAGTGGTTGTTCCATCACAAATAGTAACGATGGTCCCAAGACATGTTGTAGTTAATGAATCAGAAGAAAGTATCCAAGTTCGTCAATGTTATTTACAGGATGACTCAGTAGCCATGATCCTCATAAGCAGTAAGGAGAGGGCAGCTCTCCAGCTTCGGGATGGATTGAGCAAGAAGAGAGAATTCGGTTTATTTGAGCATATTATGAGGAAGCACAGAAAAGTTAATGAAGATTCACTAATATATCTCCAGTTTCGGTTGCATGAGTCTGAATTAGGTTGGTCAGGGCCAATATGCATTGCTTCTCTGGGAAGATTTTTCCTCAAATTCAAAAAGCCTTGTATGGACCAAGTGACAGCAGTAGAAAGCAATGTAACAGAATTTGCTGCTGTTCATGTTGTGGAAGAGGGTTCTTCTCTTGTGTTACGCTTCCATAAACCTCCAAACGTCAGTCTACCATATCGAATAGAGAATCGCTTGTGTGATGTATCTGTAACTTACTACCAGAAGGATTCATTGGAGCCGGAGATTCTTGGATCTGAAAGTGGTACAGATTATGTCTGGGATGATTCGACTCTCCCTCATAAGTTGGTTGTCAGAATCAATGATAGTCTTCTGCTACGTGAAATTAACTTGGATAAGGTGAGGGCATGGAAGCCTTTCTACAAACCAAGGCAACATAGTGGGTTGGCATCTCATTTACCATTGGGCAAGAGATCTGGAGGCCAGGGAATTGATTTTGGTGAGTTAAATGCCAGGGAGATGGTGAAGGTGGGGTATGAAGTATATGCAGACGGTCCAACTAGAGTTTTAAGATTTTGTGAGATTTCCAGGAGCCACAAGGGCGATAAACCATTTCATTCATGTCAGAAAATTCAACTAAGAGTTCCTCAATTTACAATCCACCTACTTGAACAGGAAAAAAAGGATGGAGATGAAACGGGACCTTCAGCTTATACACCAATCATAGCTGCAAGAATTGGGAATATTAACTTTGATTCTCTCTTTACTTATGAGCAAAAGTTCTGCCAGATCTGTGTACAGTCACTTAATCTGGAGTACAAGTGGGTGGGAGCTCCTTTTGCGGCAATGCTTCGTAGACATGAGTTAGACTACAATGATTCAAATGACTGTGTACTCAAAATTGTTGTTGTCTTCCTTTCAACTAGCTCCAATGTCGTACAAGTTAAATACTCATCTATTGCTCTGCAGCCAATGGATTTAAATCTTGACGAGGAGACATTGATGAAACTCGTACCATTTTGGAGAACATCTCTTAGTGACTCGAAGAGTGGGCAATATTATTTTGACCATCTTGAGATCCACCCAATAAAGATTTATGCAAACTTTCTTCCTGGGGACTCGTATTCAAGTTATAGCTCAGCTGAAGAGACTCTCAGGTCCTTACTGCACAGTGTAGTAAAGGTCCCTGCAATAAAGAATAAGGTTGTAGAGCTCAACGGCGTTATGATCACTCATGCTTTGATAACGATGCGTGAATTAGTTATCAAATGTGCACAACATTATTCATGGTATGGCATGAGGGCTGTCTATATTGCAAAAGGAAGTCCATTGCTTCCACCAGATTTTGTGTCGATTTTTGATGATCTGGCGTCATCCTCTCTTGATGTGTTTTTTGATCCTTCTCGTGGGTTAAAGAATCTCCCAGGTTTGACGTTAGGCACGTTCAAATTAATCAGTAAATGTATTGACGGCAAAGGGTTCTCGGGGACAAAACGCTATTTTGGTGATCTAGGAAAATCTTTGAGAACAGCTGGGACGAATGTACTCTTTGCTGCAGTCACAGAGATATCAGACTCTGTTTTGAAGGGTGCAGAAGCAAGTGGTTTTAACGGAGTGGTGACTGGCTTTCACCAAGGAATACTGAAATTGGCTATGGAGCCATCACTACTGGGGACTGCGTTGATGGAAGGTGGCCCAGATAGAAAGATTAAGCTTGACCGGAGTCCTGCGGCTGATGAGTTATATATTGAAGGATACTTACAAGCCATGTTGGACACTGTGTATCGACAGGAGTATCTGAGAGTCAGGGTCATTGACAATCAGGTTTACCTCAAAAACCTACCCCCAAACAGTTCCCTCATAGAGGAGATTATGGATCGGGTGAAGGGGTTTCTTGTGAGCAAGGCATTGCTAAAAGGAGATCCTTCAGCAGCATCTCGCCCGCTGAGTCGTCTTCGAGGTGAAAATGAATGGAGGCTCGGACCAACGGTGCTTACGTTGTGCGAGCACCTGTTCGTAAGCTTCGCCATCCGGTTGCTAAGGAAGCAAGCAAACAAGTTCATCTCCAACATCAAGTGGAAATCCGAAGACACCAATCCTAATGCAGTTGTCCCAGCAAGCCAACCACCCAAGTTTGTTGCCGGCCTCAAATGGGGCGTCGGCAAGTTTGTTCTGTCAGGGATTGTAGCATATGTAGACGGTCGGTTGTGTCGCTGTATCCCTAACCCTGTGGCTCGCCGCATCGTCAGCGGCTTTCTGTTGACTTTCCTTGACAACAGTAACAATAACAATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHR-BD VPS13_C

Protein Analysis

3155

Amino Acids

352.9

Weight (kDa)

6.53

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_VAB PF25036 2079 - 2497 3.3e-21 Vacuolar-sorting associated protein 13, adaptor binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 6432
AarI CACCTGC 1 cut(s) 4094
AasI GACNNNNNNGTC 1 cut(s) 8323
Acc36I ACCTGC 1 cut(s) 4094
Acc65I GGTACC 1 cut(s) 3269
AccB1I GGYRCC 1 cut(s) 3269
AccB7I CCANNNNNTGG 1 cut(s) 4589
AccI GTMKAC 4 cut(s) 6182, 7378, 8023, 9363
AccII CGCG 3 cut(s) 22, 107, 4611
AccIII TCCGGA 1 cut(s) 796
AcoI YGGCCR 5 cut(s) 47, 165, 402, 2315, 5826
AcvI CACGTG 2 cut(s) 3146, 3301
AcyI GRCGYC 3 cut(s) 258, 8537, 9324
AdeI CACNNNGTG 1 cut(s) 3149
AflII CTTAAG 4 cut(s) 2144, 2575, 6298, 6553
AhdI GACNNNNNGTC 1 cut(s) 4077
AjiI CACGTC 2 cut(s) 5094, 6603
AjuI GAANNNNNNNTTGG 6 cut(s) 1467, 1499, 1961, 1993, 7424, 7456
Alw44I GTGCAC 6 cut(s) 476, 606, 718, 2291, 3296, 8439
AlwNI CAGNNNCTG 3 cut(s) 3689, 4589, 8744
Ama87I CYCGRG 6 cut(s) 7, 279, 362, 5580, 6564, 8650
Aor13HI TCCGGA 1 cut(s) 796
ApaLI GTGCAC 6 cut(s) 476, 606, 718, 2291, 3296, 8439
ArsI GACNNNNNNTTYG 4 cut(s) 5040, 5072, 9309, 9341
AseI ATTAAT 1 cut(s) 8618
Asp700I GAANNNNTTC 5 cut(s) 3309, 3858, 4814, 4918, 7583
Asp718I GGTACC 1 cut(s) 3269
AspLEI GCGC 3 cut(s) 22, 4569, 4613
AsuC2I CCSGG 1 cut(s) 59
AsuII TTCGAA 2 cut(s) 1619, 1940
AvaI CYCGRG 6 cut(s) 7, 279, 362, 5580, 6564, 8650
AxyI CCTNAGG 2 cut(s) 2356, 2387
BaeGI GKGCMC 8 cut(s) 480, 610, 722, 2295, 3300, 4633, 6581, 8443
BaeI ACNNNNGTAYC 2 cut(s) 8945, 8978
BalI TGGCCA 3 cut(s) 404, 2317, 5828
BanI GGYRCC 1 cut(s) 3269
BarI GAAGNNNNNNTAC 8 cut(s) 1122, 1154, 3262, 3294, 7571, 7603, 8910, 8942
BauI CACGAG 3 cut(s) 749, 5217, 8571
BbrPI CACGTG 2 cut(s) 3146, 3301
BbsI GAAGAC 7 cut(s) 578, 1560, 3498, 7529, 8071, 9113, 9261
BceAI ACGGC 2 cut(s) 8401, 8653
BcgI CGANNNNNNTGC 4 cut(s) 353, 387, 6806, 6840
BciVI GTATCC 6 cut(s) 5272, 6101, 6274, 7007, 8915, 9395
BclI TGATCA 5 cut(s) 574, 1018, 1756, 2980, 8394
BcnI CCSGG 1 cut(s) 59
BfmI CTRYAG 6 cut(s) 2307, 3681, 4830, 5239, 8132, 8721
BfrI CTTAAG 4 cut(s) 2144, 2575, 6298, 6553
BfuAI ACCTGC 1 cut(s) 4094
BfuI GTATCC 6 cut(s) 5272, 6101, 6274, 7007, 8915, 9395
BglI GCCNNNNNGGC 1 cut(s) 7767
BglII AGATCT 3 cut(s) 5537, 7640, 7956
BlpI GCTNAGC 2 cut(s) 5760, 8305
BmcAI AGTACT 2 cut(s) 5344, 5586
BmeRI GACNNNNNGTC 1 cut(s) 4077
BmeT110I CYCGRG 6 cut(s) 7, 279, 362, 5580, 6564, 8650
BmgBI CACGTC 2 cut(s) 5094, 6603
BmrI ACTGGG 5 cut(s) 144, 4693, 5150, 6336, 8846
BmuI ACTGGG 5 cut(s) 144, 4693, 5150, 6336, 8846
BoxI GACNNNNGTC 2 cut(s) 2055, 8889
BpiI GAAGAC 7 cut(s) 578, 1560, 3498, 7529, 8071, 9113, 9261
BplI GAGNNNNNCTC 6 cut(s) 5133, 5165, 7054, 7086, 8182, 8214
BpmI CTGGAG 8 cut(s) 1291, 1392, 6693, 6894, 7058, 7157, 7665, 8000
Bpu10I CCTNAGC 1 cut(s) 9206
Bpu1102I GCTNAGC 2 cut(s) 5760, 8305
Bpu14I TTCGAA 2 cut(s) 1619, 1940
BpuEI CTTGAG 5 cut(s) 223, 1226, 2867, 3884, 8261
BpuMI CCSGG 1 cut(s) 59
Bsa29I ATCGAT 2 cut(s) 2892, 6313
BsaAI YACGTR 4 cut(s) 3146, 3301, 6260, 7547
BsaBI GATNNNNATC 5 cut(s) 759, 1609, 4374, 6240, 8148
BsaHI GRCGYC 3 cut(s) 258, 8537, 9324
BsaI GGTCTC 2 cut(s) 4064, 5569
BsaWI WCCGGW 5 cut(s) 123, 796, 5726, 8886, 9198
Bse118I RCCGGY 2 cut(s) 1123, 9308
Bse21I CCTNAGG 2 cut(s) 2356, 2387
Bse3DI GCAATG 9 cut(s) 2138, 2724, 3265, 4562, 7221, 7300, 8019, 8495, 9074
Bse8I GATNNNNATC 5 cut(s) 759, 1609, 4374, 6240, 8148
BseAI TCCGGA 1 cut(s) 796
BseCI ATCGAT 2 cut(s) 2892, 6313
BseJI GATNNNNATC 5 cut(s) 759, 1609, 4374, 6240, 8148
BseMI GCAATG 9 cut(s) 2138, 2724, 3265, 4562, 7221, 7300, 8019, 8495, 9074
BseRI GAGGAG 6 cut(s) 77, 299, 4421, 5691, 8174, 9047
BseSI GKGCMC 8 cut(s) 480, 610, 722, 2295, 3300, 4633, 6581, 8443
BseX3I CGGCCG 1 cut(s) 165
BseYI CCCAGC 2 cut(s) 8699, 9280
BsgI GTGCAG 5 cut(s) 3126, 4452, 5085, 8318, 8778
Bsh1236I CGCG 3 cut(s) 22, 107, 4611
Bsh1285I CGRYCG 3 cut(s) 111, 168, 9370
BshNI GGYRCC 1 cut(s) 3269
BshVI ATCGAT 2 cut(s) 2892, 6313
BsiEI CGRYCG 3 cut(s) 111, 168, 9370
BsiHKCI CYCGRG 6 cut(s) 7, 279, 362, 5580, 6564, 8650
BsiSI CCGG 9 cut(s) 58, 124, 797, 1124, 5727, 7448, 8887, 9199, 9309
BsmBI CGTCTC 1 cut(s) 6005
BsmI GAATGC 2 cut(s) 6046, 6702
Bso31I GGTCTC 2 cut(s) 4064, 5569
BsoBI CYCGRG 6 cut(s) 7, 279, 362, 5580, 6564, 8650
Bsp119I TTCGAA 2 cut(s) 1619, 1940
Bsp13I TCCGGA 1 cut(s) 796
Bsp1407I TGTACA 6 cut(s) 490, 785, 1138, 2821, 3749, 7963
Bsp1720I GCTNAGC 2 cut(s) 5760, 8305
Bsp19I CCATGG 3 cut(s) 50, 1956, 5823
BspDI ATCGAT 2 cut(s) 2892, 6313
BspEI TCCGGA 1 cut(s) 796
BspFNI CGCG 3 cut(s) 22, 107, 4611
BspHI TCATGA 1 cut(s) 2983
BspMAI CTGCAG 5 cut(s) 2311, 3685, 5243, 8136, 8725
BspMI ACCTGC 1 cut(s) 4094
BspQI GCTCTTC 1 cut(s) 158
BspT104I TTCGAA 2 cut(s) 1619, 1940
BspT107I GGYRCC 1 cut(s) 3269
BspTI CTTAAG 4 cut(s) 2144, 2575, 6298, 6553
BspTNI GGTCTC 2 cut(s) 4064, 5569
BsrDI GCAATG 9 cut(s) 2138, 2724, 3265, 4562, 7221, 7300, 8019, 8495, 9074
BsrFI RCCGGY 2 cut(s) 1123, 9308
BsrGI TGTACA 6 cut(s) 490, 785, 1138, 2821, 3749, 7963
BssAI RCCGGY 2 cut(s) 1123, 9308
BssNI GRCGYC 3 cut(s) 258, 8537, 9324
BssSI CACGAG 3 cut(s) 749, 5217, 8571
BssT1I CCWWGG 6 cut(s) 50, 1956, 4861, 5823, 7595, 8800
Bst2BI CACGAG 3 cut(s) 749, 5217, 8571
Bst6I CTCTTC 8 cut(s) 158, 1025, 2927, 6485, 7094, 7323, 8207, 8307
BstACI GRCGYC 3 cut(s) 258, 8537, 9324
BstAFI CTTAAG 4 cut(s) 2144, 2575, 6298, 6553
BstAPI GCANNNNNTGC 1 cut(s) 1178
BstAUI TGTACA 6 cut(s) 490, 785, 1138, 2821, 3749, 7963
BstBAI YACGTR 4 cut(s) 3146, 3301, 6260, 7547
BstBI TTCGAA 2 cut(s) 1619, 1940
BstDSI CCRYGG 4 cut(s) 50, 1956, 5823, 6906
BstEII GGTNACC 2 cut(s) 4255, 4856
BstENI CCTNNNNNAGG 2 cut(s) 4865, 9029
BstFNI CGCG 3 cut(s) 22, 107, 4611
BstHHI GCGC 3 cut(s) 22, 4569, 4613
BstMCI CGRYCG 3 cut(s) 111, 168, 9370
BstPAI GACNNNNGTC 2 cut(s) 2055, 8889
BstPI GGTNACC 2 cut(s) 4255, 4856
BstSFI CTRYAG 6 cut(s) 2307, 3681, 4830, 5239, 8132, 8721
BstSLI GKGCMC 8 cut(s) 480, 610, 722, 2295, 3300, 4633, 6581, 8443
BstUI CGCG 3 cut(s) 22, 107, 4611
BstV2I GAAGAC 7 cut(s) 578, 1560, 3498, 7529, 8071, 9113, 9261
BstXI CCANNNNNNTGG 1 cut(s) 7687
BstZI CGGCCG 1 cut(s) 165
Bsu15I ATCGAT 2 cut(s) 2892, 6313
Bsu36I CCTNAGG 2 cut(s) 2356, 2387
BsuI GTATCC 6 cut(s) 5272, 6101, 6274, 7007, 8915, 9395
BsuTUI ATCGAT 2 cut(s) 2892, 6313
BtgI CCRYGG 4 cut(s) 50, 1956, 5823, 6906
BtrI CACGTC 2 cut(s) 5094, 6603
BtsI GCAGTG 3 cut(s) 5236, 6078, 6218
BveI ACCTGC 1 cut(s) 4094
CaiI CAGNNNCTG 3 cut(s) 3689, 4589, 8744
CciI TCATGA 1 cut(s) 2983
CfoI GCGC 3 cut(s) 22, 4569, 4613
Cfr10I RCCGGY 2 cut(s) 1123, 9308
ClaI ATCGAT 2 cut(s) 2892, 6313
CseI GACGC 6 cut(s) 247, 2158, 5176, 5490, 8526, 9313
DraI TTTAAA 5 cut(s) 297, 430, 1203, 2422, 8148
DraIII CACNNNGTG 1 cut(s) 3149
DrdI GACNNNNNNGTC 1 cut(s) 8323
DriI GACNNNNNGTC 1 cut(s) 4077
DseDI GACNNNNNNGTC 1 cut(s) 8323
EaeI YGGCCR 5 cut(s) 47, 165, 402, 2315, 5826
EagI CGGCCG 1 cut(s) 165
Eam1104I CTCTTC 8 cut(s) 158, 1025, 2927, 6485, 7094, 7323, 8207, 8307
Eam1105I GACNNNNNGTC 1 cut(s) 4077
EarI CTCTTC 8 cut(s) 158, 1025, 2927, 6485, 7094, 7323, 8207, 8307
Ecl136II GAGCTC 6 cut(s) 151, 633, 2831, 5759, 8000, 8380
EclXI CGGCCG 1 cut(s) 165
Eco130I CCWWGG 6 cut(s) 50, 1956, 4861, 5823, 7595, 8800
Eco31I GGTCTC 2 cut(s) 4064, 5569
Eco32I GATATC 8 cut(s) 484, 550, 2896, 3012, 3485, 4195, 4530, 8736
Eco52I CGGCCG 1 cut(s) 165
Eco53kI GAGCTC 6 cut(s) 151, 633, 2831, 5759, 8000, 8380
Eco72I CACGTG 2 cut(s) 3146, 3301
Eco81I CCTNAGG 2 cut(s) 2356, 2387
Eco88I CYCGRG 6 cut(s) 7, 279, 362, 5580, 6564, 8650
Eco91I GGTNACC 2 cut(s) 4255, 4856
EcoICRI GAGCTC 6 cut(s) 151, 633, 2831, 5759, 8000, 8380
EcoNI CCTNNNNNAGG 2 cut(s) 4865, 9029
EcoO109I RGGNCCY 4 cut(s) 2871, 7868, 8325, 8350
EcoO65I GGTNACC 2 cut(s) 4255, 4856
EcoRI GAATTC 4 cut(s) 3549, 4313, 5938, 7106
EcoRV GATATC 8 cut(s) 484, 550, 2896, 3012, 3485, 4195, 4530, 8736
EcoT14I CCWWGG 6 cut(s) 50, 1956, 4861, 5823, 7595, 8800
EcoT22I ATGCAT 5 cut(s) 4012, 5171, 6129, 6802, 7223
ErhI CCWWGG 6 cut(s) 50, 1956, 4861, 5823, 7595, 8800
Esp3I CGTCTC 1 cut(s) 6005
FalI AAGNNNNNCTT 4 cut(s) 423, 455, 4126, 4158
FauI CCCGC 3 cut(s) 4420, 5794, 9117
FauNDI CATATG 1 cut(s) 9358
FbaI TGATCA 5 cut(s) 574, 1018, 1756, 2980, 8394
FblI GTMKAC 4 cut(s) 6182, 7378, 8023, 9363
GlaI GCGC 3 cut(s) 21, 4568, 4612
GsaI CCCAGC 2 cut(s) 8703, 9284
GsuI CTGGAG 8 cut(s) 1291, 1392, 6693, 6894, 7058, 7157, 7665, 8000
HapII CCGG 9 cut(s) 58, 124, 797, 1124, 5727, 7448, 8887, 9199, 9309
HgaI GACGC 6 cut(s) 247, 2158, 5176, 5490, 8526, 9313
HhaI GCGC 3 cut(s) 22, 4569, 4613
Hin1I GRCGYC 3 cut(s) 258, 8537, 9324
Hin6I GCGC 3 cut(s) 20, 4567, 4611
HinP1I GCGC 3 cut(s) 20, 4567, 4611
HincII GTYRAC 5 cut(s) 1314, 4600, 5097, 6354, 9429
HindII GTYRAC 5 cut(s) 1314, 4600, 5097, 6354, 9429
HpaI GTTAAC 1 cut(s) 1314
HpaII CCGG 9 cut(s) 58, 124, 797, 1124, 5727, 7448, 8887, 9199, 9309
Hpy99I CGWCG 6 cut(s) 27, 161, 542, 3728, 5757, 9329
Hsp92I GRCGYC 3 cut(s) 258, 8537, 9324
HspAI GCGC 3 cut(s) 20, 4567, 4611
Kpn2I TCCGGA 1 cut(s) 796
KpnI GGTACC 1 cut(s) 3273
KroI GCCGGC 2 cut(s) 1123, 9308
KroNI GCCGGC 2 cut(s) 1125, 9310
Ksp22I TGATCA 5 cut(s) 574, 1018, 1756, 2980, 8394
KspAI GTTAAC 1 cut(s) 1314
LguI GCTCTTC 1 cut(s) 158
MfeI CAATTG 6 cut(s) 1002, 4700, 5359, 5556, 5688, 6741
MlsI TGGCCA 3 cut(s) 404, 2317, 5828
MluNI TGGCCA 3 cut(s) 404, 2317, 5828
MmeI TCCRAC 9 cut(s) 440, 895, 2373, 4147, 5542, 6144, 7748, 8919, 9258
Mox20I TGGCCA 3 cut(s) 404, 2317, 5828
Mph1103I ATGCAT 5 cut(s) 4012, 5171, 6129, 6802, 7223
MroI TCCGGA 1 cut(s) 796
MroNI GCCGGC 2 cut(s) 1123, 9308
MroXI GAANNNNTTC 5 cut(s) 3309, 3858, 4814, 4918, 7583
MscI TGGCCA 3 cut(s) 404, 2317, 5828
MslI CAYNNNNRTG 6 cut(s) 1440, 2204, 2484, 3776, 4104, 5828
Msp20I TGGCCA 3 cut(s) 404, 2317, 5828
MspA1I CMGCKG 6 cut(s) 2189, 2642, 8309, 8699, 9112, 9417
MspCI CTTAAG 4 cut(s) 2144, 2575, 6298, 6553
MspI CCGG 9 cut(s) 58, 124, 797, 1124, 5727, 7448, 8887, 9199, 9309
MunI CAATTG 6 cut(s) 1002, 4700, 5359, 5556, 5688, 6741
Mva1269I GAATGC 2 cut(s) 6046, 6702
MvnI CGCG 3 cut(s) 22, 107, 4611
NaeI GCCGGC 2 cut(s) 1125, 9310
NciI CCSGG 1 cut(s) 59
NcoI CCATGG 3 cut(s) 50, 1956, 5823
NdeI CATATG 1 cut(s) 9358
NgoMIV GCCGGC 2 cut(s) 1123, 9308
NmeAIII GCCGAG 1 cut(s) 193
NsiI ATGCAT 5 cut(s) 4012, 5171, 6129, 6802, 7223
NspV TTCGAA 2 cut(s) 1619, 1940
PaeI GCATGC 1 cut(s) 4103
PaeR7I CTCGAG 2 cut(s) 5580, 6564
PagI TCATGA 1 cut(s) 2983
PaqCI CACCTGC 1 cut(s) 4094
PasI CCCWGGG 1 cut(s) 5636
PciI ACATGT 8 cut(s) 1557, 1806, 2164, 4296, 4383, 5010, 6289, 6967
PciSI GCTCTTC 1 cut(s) 158
PcsI WCGNNNNNNNCGW 5 cut(s) 153, 165, 5752, 8606, 9161
PctI GAATGC 2 cut(s) 6046, 6702
PdiI GCCGGC 2 cut(s) 1125, 9310
PdmI GAANNNNTTC 5 cut(s) 3309, 3858, 4814, 4918, 7583
PflMI CCANNNNNTGG 1 cut(s) 4589
PfoI TCCNGGA 3 cut(s) 57, 4582, 7754
PmaCI CACGTG 2 cut(s) 3146, 3301
PmlI CACGTG 2 cut(s) 3146, 3301
Ppu21I YACGTR 4 cut(s) 3146, 3301, 6260, 7547
PpuMI RGGWCCY 4 cut(s) 2871, 7868, 8325, 8350
PscI ACATGT 8 cut(s) 1557, 1806, 2164, 4296, 4383, 5010, 6289, 6967
PshAI GACNNNNGTC 2 cut(s) 2055, 8889
PshBI ATTAAT 1 cut(s) 8618
PsiI TTATAA 1 cut(s) 6432
Psp124BI GAGCTC 6 cut(s) 153, 635, 2833, 5761, 8002, 8382
Psp5II RGGWCCY 4 cut(s) 2871, 7868, 8325, 8350
PspCI CACGTG 2 cut(s) 3146, 3301
PspEI GGTNACC 2 cut(s) 4255, 4856
PspFI CCCAGC 2 cut(s) 8699, 9280
PspPPI RGGWCCY 4 cut(s) 2871, 7868, 8325, 8350
PspXI VCTCGAGB 1 cut(s) 5580
PsrI GAACNNNNNNTAC 4 cut(s) 1557, 1589, 5035, 5067
PstI CTGCAG 5 cut(s) 2311, 3685, 5243, 8136, 8725
PstNI CAGNNNCTG 3 cut(s) 3689, 4589, 8744
PvuII CAGCTG 4 cut(s) 2189, 2642, 8309, 8699
RseI CAYNNNNRTG 6 cut(s) 1440, 2204, 2484, 3776, 4104, 5828
SacI GAGCTC 6 cut(s) 153, 635, 2833, 5761, 8002, 8382
SapI GCTCTTC 1 cut(s) 158
ScaI AGTACT 2 cut(s) 5344, 5586
SfcI CTRYAG 6 cut(s) 2307, 3681, 4830, 5239, 8132, 8721
Sfr274I CTCGAG 2 cut(s) 5580, 6564
SfuI TTCGAA 2 cut(s) 1619, 1940
SlaI CTCGAG 2 cut(s) 5580, 6564
SmiI ATTTAAAT 2 cut(s) 2422, 8148
SmiMI CAYNNNNRTG 6 cut(s) 1440, 2204, 2484, 3776, 4104, 5828
SphI GCATGC 1 cut(s) 4103
SspI AATATT 5 cut(s) 1693, 3388, 4969, 7912, 8225
SstI GAGCTC 6 cut(s) 153, 635, 2833, 5761, 8002, 8382
StyI CCWWGG 6 cut(s) 50, 1956, 4861, 5823, 7595, 8800
SwaI ATTTAAAT 2 cut(s) 2422, 8148
TauI GCSGC 5 cut(s) 49, 8012, 8900, 9408, 9420
Van91I CCANNNNNTGG 1 cut(s) 4589
Vha464I CTTAAG 4 cut(s) 2144, 2575, 6298, 6553
VneI GTGCAC 6 cut(s) 476, 606, 718, 2291, 3296, 8439
VspI ATTAAT 1 cut(s) 8618
XagI CCTNNNNNAGG 2 cut(s) 4865, 9029
XbaI TCTAGA 1 cut(s) 5107
XcmI CCANNNNNNNNNTGG 4 cut(s) 2559, 4267, 4699, 7082
XhoI CTCGAG 2 cut(s) 5580, 6564
XmiI GTMKAC 4 cut(s) 6182, 7378, 8023, 9363
XmnI GAANNNNTTC 5 cut(s) 3309, 3858, 4814, 4918, 7583
ZrmI AGTACT 2 cut(s) 5344, 5586
Zsp2I ATGCAT 5 cut(s) 4012, 5171, 6129, 6802, 7223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.