RLG00000017225

regulation of parkin-mediated stimulation of mitophagy in response to mitochondrial depolarization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004207
Physical Location & Seq
Reverse (-)
14293319 .. 14316828
23510 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017225

Sequence Viewer

Length: 9702 bp
ATGTTTCCGAACGGCACAATCCGGCGGAGGCTGGCCGCTCTGCTGCGGCCGTGGCTTCGGGAAGAGCCGGAACTCGAGCTCAGTTTGGGGCTGATCAGTTCGCACGCTGTGGCTAAGAACCTCCGATTCGATACCGCGGCGCTCAATCGGCTCGGCGACGATTCGGACGGGTTTTTGTATAAGGCGGTCAGTGTCGAACAGTTGAGCGTTCGGTTCTCGAACTGGCTCGCTCCTGCATTTTCAATTGAGTTTCATGGCGTCAAAGTGGTGCTCTCGACTCGGGAGTTGAAGAAGAAGAAGCTTTCTGAGGATACATTTGCAGAGGATATGAAGAAGAAGAAGCTTTCTGAGATTGATCCTCAGGGGAGTGCTGTGCATGCTTTTCTGGAAAAACTTATGGCTGCTTCTACGACTCCAAAAAATAAGTTCAAAACATCTCTTTTGAATCTGTTACTCACACACTGTCAGCTTCAAGTGCATGATATTAGTGTCCAAGTCCACGTTCCGATCTTAAATGATTCCTTTGTCTGTTCACTGGATATAGAAGATATAAATGTAGATCCTCAGTATCTTGATCGTGGGTGTCTTCTTCGAGGCCTTTTTGGTACATTCTTTGTACCTATGAAGGAGAGCTCTTTTGCTATAGTAGGCAGTGGTTGTGAGGTTGGTTTTAAGAGGGCAGGTCAACTCAAACGTGTCTTGTTATTGACTGAGTTGTGCAGTTGCATTAAGTTGAATGATTTTCAGCTTGTAGACATCAATCTTCGAGTCCCTGAAATACATTTTTCGTTTTCTCCGGATGATGTTTCTTTCTATTTGGCATTCACTACAGCATCATCCCAAGAATCTCATCGTGCTAGAGATGGCAGACAACTGTGGAAGCTAGCTGCGAGTAGAATTGACAATATGACTTCAGCTCATAGGTGGTCGTTGCAAAAGTCAGCTGTTGCTGTTTGTTTCTGGTTACGTTATCTCAATGCTTATGAGTATTTACTGTTGCTAATTGGATATTATGACGACCACTTGTTAAAAAGATCAGCTATTAGAATCTATGAGGACAAGATGCTTTTGAGTTCTGTTGAAATCCAATGGAAGGTGATTTCTGATATTGAGAAAGAGCTGCCAGCTGAAGCTATTGCACAGGCATGGAGAGTAGCCCGTAACAGAGCAGCATCAAATGTTCAATGTCCCGAATATAGTTCCAAAAAACCATTTATGACTACAATTTGCAACTTCCTTCTCATAATCCTTTCATTACTAGCTTGTGCCTGGAGATTTTTTTGCAAGATAGTCCTTTTTATTATGCACCCCTTTGTTTTTAGGAAAATATTAGTCAATGAGCCAAAGAGTGCAGATCTGGATATTGTTTCTGAAGGTCCGTGTACGCAGTTTTGCTTCAGTTTGATGCTTGGAAAATTTCAACTCACTATCTCTCATGGAAATGAAATTCAACTCTTAGTAAATGAAAAGTTGAAATCACATCTTGGGATCACTTACTCGGATTCACTTTCATTTCGTCTTTCAGTTGATGCACTGTTATTAAAATATGTAGACGACATGTGTGAAGAGTCTTTACTGATATCCTGTGGGCAATTAAAGGTCAGATCCTCATCTCTTATGGAAGCTCCTGTTAAAGAAAGCAGTTCAAAATTATCCTTTAGTTCTATGGAAGCACATTGGAAGGAGAGCAATGATAAGTGGAACAATATTCTGTGGGGTGAGCCAGCAGAAATATTTCCACTTTTAGAAACTTCTGAAACTGGTTGTGCTGATCATGTGGAAGGTTCTTGTGTCTCATTTCTGAAAGACATGTGGTTGGATTGGAGAAGTGAGTGCGATAAATTTGGAAAAAGTGAAATTCAGTACTCTGAAACTCCATTCCTTCTTTGTGAATTTAAAAACTTCTTGATATATCCAGACCTCAAAACATCGGATTCTGGTTTTTGGAAGTTCTATTTTGTTCTGGGAAAGTTGAATCTTGTGTTGGGATATTCATCAATAGTATCATTGTCTCTGCTATTTAGGCAGATACAACGTACTCTATGCGGGGGAGAGGCCAATGGTCAGTCACCCGGTCTTTCACATTCTCCAAGAACCAGTGAGAGTAAACCAGAAATCAGTCTGGATAGCAAATACAAGTGCTATGCTAATAGATTAGAAATGGCATTGCTTAAAATGCTTCCAAAAAAACATGTCCAACTAGGGGTATTTATTGCTGGTCCTCACATCCTTATGTCATTGGGAAAGAATTTTGATGGTGGCAACAAAGACAAAAATCATGTATATATTCAGGACGATTTTCACTTTGCATTTGATGTGCACAATATTGAAGCTGCTGTTTGGCCAACCTCAAAGTTTGATCTTGCATCATTCGTGGGGCCCTCAGCATCTGATGATGTGGAACCAGAGTGCCTCAGGATGGACCAGCCACTGGTGATTGATATCTCTAACTCTAATAATGGGAAGTATCAAGCTCAGGGTGGGATCTCTCTTGGTTCCTACATACGAGTTGATGGTTTAGATGTCTGCCTGGTCGCCGCAGCAGGAAAACGACAAAGCCAGATTTTGGTTTCTAAGCCAATGACTTTGCAGTTGTTATCCTCCAGGGAGTATGTTCATTCATTTAGCACAAATGTTATTGCTTTCTCTGCAGCTTTGTTTGGGAAAACTGAAGGATTCACTATTTTATCGTACATGGATGAGTTTGATGTTCTCTTTCAGGTGCTTGCAAATTTATCTTCTGCAGTATCATATTCATTTAGACATTTTGCATCTGTCAGTGACTTGCCTTTACAATTTGGAAAGCAACAATATTCAAATGCTGAATCTGAGAACGAGCAGACCACAGCGCAAGAACCTCCCTTGAATTATAGCAGTGTCCTATTTTCAACTAATGGGACCTTCAAAATCAAGTCAATGAATATAATCGTGCACAAGTCTAGGATGTGTGATAATGTGGGGAGCTGCCTAGTAACCTCTGATGCTTCAAGTAGCAAACAGTTGTCTGAGCATGACTTGCCTGATTATGGAATTTGGATTTCAGTTCATCAAACAAGTCTTGATATGTCTTGCAAAGAAGGAAAAGTGATACTTCTTTCTAATTTATCAGAAATTCATTCTTCAAGTTTTAGATATAAGAATCGGAGGGGCAAAAGTACTGATCAGTCTGTACTTAGTGATCTGCTACTGCAATCTCTCGATTGCATATATCAACTATCACTTTCTAGTTGTGTACTTAGTTTGGCACTGTTCCTATCTCAGAACTGCTTATCTATTGGCACTGTTAGCAACACACTTGATACTAATTCTGTGAGTGAAACAGAGCACAAGGAAAATTTCCCATTCACTAATTCTGAAAGCTCAGGTCATCAGAACTATAGCTTTATTCAAGGTTCAGAATTGGCTTCAAATATTCGACCACCGGGCTTAAGCCATTGGTTACTTGTAAATCTTGCACTGGGTAAGATATATGTGGGAAGGTGCTCAGCAAAGAATGTTACGAATGAGGCGCATCAATTAAATAAGTTTTTGTCATCAGTTTCTGTTGGTGGTGAATTTCAGAGAATCAGTTGTGAAATTCAGGGTGGTTTCCTTTTCCTTGAAACAACAGCTTCGGCTACCTTATTGCGATGTTTTTCTTCATATCTTGATTGCTTCACAAATATTTTATCTGGCTCACAATCATCTGATAAGCACGTTGAAGAAGATAAGCCCATTGAAGAAGCTGATACTGCTGTAAATATGACTAGACCAGATAGTCATTCTGATAAGGACTGTATGCAAGAGACTCCTTGTACATCTCCACAGGTCAAAAGGGGACAGATGGAAGCTTTTAGCTTAAATATATCTCGTTTTTCTTGCGCTCTTGTGATTCAGGAAGAGAATGGTGCTGTACAGGAGCTCGTGCTTGAGATTGATGCCCATTTGAACGTTGAGTTGGAAAACATGCGAAGAAAATTTATATTTAAGCTTTCTCACATATCAATCCTTTCTCAGGTCCTTCAAGAAATTTTGGAAAATCAAACCCAAAGTTCTCAAGTTTCTTCAGTCACATCGAATATTTTTCAATCTCATGCTGCATCAGGAGCATCTACAGGTTCTCAGCACATGGATGAGATTCATCCTGTTAATAATGCAAGTAGTTCAAGAGGTCCTGGATCACAGGAAGAGCGCTCTGAACATAGTTCTCTGCATGAGGTATTTCATCAAAGTCATCACAAATATATCCTAAAGGGTCAAGAATTAGCCTCCAGTGAGTGTGAGTCAAGACAGGAAGGGGGAACTGCCTTCATATCAGTTGAGAAGCCTTTAAATGAAGTTTGGGTTGGAAGCGGAACTATTTCATGTTTCAATATTACAATATCTCTATCTCAAATACAGATGCTTTTGTCTATGATTTCATCCTTTTCTGGGGTATTTGGGGAGGAGGTGATCAGTGAACCGGATAGAAGGCACTGGTCGAGTGACGAGGAATTAAAAAACAGTTTGGAGACAATGGTTCCTAATGGTGCAATAGTGGCAATTCAGGATGTCCATCAACATATGTATTTTACAGTTGAGGGTAATGAGGATAAGTACAGCTTAGCGGGTGCAGTTCATTATTCTCTTGTGGGAGAAAGCGCTCTTTTCATGGTCAAGTATAACAATCAAAAGGGATGGAATTCATCAGTTTTGTGGTTCTCCTTGATATCGTTGCATGCCAAGAATGCTTCTGGGGAGCCATTGCGCTTGAACTACTGTCGAGGATCTGATTTTGTTGACGTCTCTAGTGCGAATGATAATGCAGGGGCACTTTGGACAGCGGTTTCTTGTGAGCCTGAAAATTATGAGGGCGACATTGACTGGGAGCCCTACAATCAATTGATTAAAAGGACCTTTTACCTTGTGAACAAAAAGAATGACTCTGCTGTTGCTATTGTAGATGGGATTCCAGAGTTTGTTAGAAAGCCTGGAAATCCAATCAAGTTGAAAGTGTTCCACAATGCTTCTATAGCTTATAACATAAAGATGGATAACTACCCTGTTGAGGCATCTGTGGCTCGTCCGCAACAGAGCGATGAAGGTTTAACTTCTGGGAAGGGTGGAAAGCTTCCTTGCATTGATGTTACATTTGACACGATTTCCTTGACCATTATCCATGAACTCGTAGATACAAAAGACATGCCTCTCCTTCGTTGTTGCATTGGCGGAACTGGACAGTCCAAACATGAACTTGAAGAGTCAAAAGACATGGCTCTCCTTGGTGGTTGCAGTGACAGAACCGAACGATCCAAACTTACTGTTCAAATTCTCCCTTCTAAAGTTAGGACTATAAGCTCCTTAACAACAGTGGTATATTACTTTGATGCACAAAGAAACAAATGGAAAGAACTTATCCACCCAGTTGAAACATGCCTTTTCTATCGTTCAAGCTTCCAAATTCAGAGTTCAGAAGCTGTTTCGCATGGAGTACCTGTTCATATCCATTGCAGAAGTAAAGAGTTGAATATATCCCTATCCGAGCTATCATTGGACATACTTCTCTTTACGGTTGGGAAACTAAATTTAGCTGGTCCTTTCTCAGTGAGAAGTACTAAGATTTGGGCCAATTGTTGCAAGGTGGAGAACCAGTCAGGATTAAACCTTCTTTGTCAGTATGATGAGCAAAGTGTAAAAGTTTCCAGAAGGCAATCTACGTCCATTATTTTAAGGTGCTCGGATTCGGAAAACCAACCTCCAGAGATGGCATCAGTCATGTCAATCCGACTTACTGGTTCTAGATCTTCTTTGATGACCTCTCCAATTCATGTCTCTCACCTAGAAGCTCAAGCATTTGCTTGGAGAACACAAATAATGTCACTTAAAGATTCACAAACTTACCCTGGACCATTTGTTATAGTTGACGTTTCAAGGAAATCCCAGGATGGTTTGTCAATTCTTATTTCTCCTCTCGTAAGAATACACAATGAAACTGGATTATCTCTAAAGCTACGATTTCGACGATCTCAGCAGAAGGAAGACGCGTTTTCTTCAGTGGTGCTTAATGCTGGTGATACATATGATGACTCCATGGCCATGTTTGATGCTATAAATTTGTCTGGTGAAGAAAAGAAGGCCCTGAGGTCTTTAAGTCTTGGCAATTTCTTGTTCTCTTTTAGACCTGAAATTCCAGATGGCTTAATGAACTCTAAGAAATCAATTTCAGCAGAATGGTCAGATGATCTTAAAGGTGGAAAGGCAGTTCGTCTCTCTGGAATCTTTGATAAATTAAGCTATAAAGTGCGGAAGGCTTTGCTTAGTGAATCTGCAAAATCTTCCTTCAGCACTGCCCATTGTACTCTTAAATCGGAAGGGGCAAATACTGTCGACATGCATTTTTTGATTCAGAGTATAAGGAGAAATGTTCCTATTGTACAACCTGATAGGTCTATGAATCTCCTTGAAAATAGGAAATCACCAGTTGCCTTAGAAGAGCAAAAGGATATTTATCTTCTTCCTACAGTGTGTGTTTCCAATTTATTGCATACGGAGATACATGTCTTTGTAAGTGAATCAGATGGACGTCCTACTATTGCGTCTGACAATGATAGGAATCAATCAAACATATCTTGTGGTTCCATGGTTGAATTTTATGCCAACCCTTCCATTATTTACTTCACAATCACACTGACTGGTTATGATGCAAGCTGCAAACCAGTGAACAGCAGTGATTGGGTCAAAAAGTTACTGAAGCAGAAAAGTGATGTGCCTTCTGTGGACATTGATCTGGATTTTGGTGGGGGAAAGTATTCTGCTACCTTAAGATTGTCTCGAGGGAGCAGAGGCACATTAGAGGCTGCTATTTTCACATCATACGCCCTGAAAAATGATACAGAATTTGGCCTTTGTTTCTTTGTGTCTAATAAGAGGCCCCTTTCTAGGGATGAAGCAGAGAAGTATGGTTCTAGCATTCCTTCAGAATTTGGATTATTTTTGCCTCCAAAGTCAACCAGATCATGGTTCTTAAAATCCAGCAAAGTGCGTCTCAAATTGCTAAAAGATAATGCATCAGGGACACTTATCGACTTGGATGCTTTATCAGGACTTGCAGAGATAAGTTTGGAAACAGAGGAAGGATCTGGAATCAGATCTATTACAAAGCTTGGGGTTTCCACAGGACCACCTTTGAGTAAAGTGGTTGTGCCATCACAAATAGTAACTATGGTCCCAAGACATGTTGTAATAAATGAATCAGGAGAAAACATCATTGTTCGCCAATGTTATTTGCAGGATGACACTGTTGGCATGATCCCTGTAAACAGTAAACAGAGGGTGCCTCTACAGCTGTGGAATGTGATAGACAAGAAGAGAGATGTCAGTTTGTTTGAGCGTGTTATGAAAAAGCACAGAAAAGCTAATGATAATTCACCAATATATCTCCAGTTTCGATTGAATGAGTCTAAGTTAGGTTGGTCGGGGCCAGTATGCATTGCTTCTCTAGGAAGATTTTTCCTCAAGTTCAAAAGACAGCAATTGGACCAAGTGACAGCACTACAAAGCAATGTAACAGAATTCGCTTCTGTTCATCTTGTTGAAGAAGGCTCTACCCTTGGTTTACACTTCCATAAACCTCCAAATGTCAGTCTCCCATACCGAATTGAGAATTGCTTGCCTGATGTGTCAATAACTTATTACCAGAAGGATTCATCAGAGCCAGAGATTATTGGATCGGAAAGTTGCACCGATTATGTTTGGGATGATTTGACTCTTCCACATAAGTTGGTCGTCAGGATCAATGATAGTCTTCTACTACGTGAAATTAACTTGGATAAGGTGAGAGCATGGAAGCCTTTCTACAAAACCAGGCAACGGAGTGGATTGGCAACCCATTTTCCATTCGGAAAAGACTCGGGGGACAAGAAGGGTGATTTTGGTGAGTTTAATGGCATGGAGATGATGAAGGTGGGGTTTGAAGTATATGCAGACGGTCCAACCCGGGTCTTAAGATTTTGTGAAATTTCTAGCAGCCACAAGGGAGATAAAATGTTTCAATCATGCAAGAAGATTCAACTAAGATTTAATCAGTTTACAGTTCACCTTCTTGAACATGAAAAACAGGATGGAGAAGATATGGAGCCGCCTGGGTATACACCAATCTTAGCTGCAAGAATTGGGAATATTTACTTTGATTCTGTCTTTACATATGAGCAAAAGTTTAGCCAGATCAGTGTACAGTCATTGAATTTGGAGAACAAGTGGGTGGGTGCTCCTTTTGCAGCAATGCTTCGTAGAGATCAATCAGACTTCGATGATTCAAATGATTGTGTACTCAAAATTGTGATTGTCTTGCTGTCAACCAGTTCCAATGTTGTACAAGTTAAGTACTCGTCTATTGCTCTGCAGCCAATGGATTTAAATCTTGATGAGGAGACGTTAATGAAAATTGTCCCATTTTGGAGAACATCTCTTAGTGAGGCGAAGAGTAGCCAGTATTATTTTGATCATTTTGAGATCCATCCAATAAAGATTATTGCAAACTTTCTTCCTGGCGAGTCATATTCAAGTTATAGCTCAACTGAGGAGACTCTAAGGTCCTTGCTGCACAGTGTAGTTAAGGTCCCTGCAATAAAAAATAAGGTTGTAGAGCTCAATGGTGTAATGGTCACTCATGCTCTTATAACGATGCGCGAATTACTTATCAAATGCGCACAACATTATTCATGGTATGCCATGAGGGCTATATATATTGCAAAGGGAAGTCCATTGCTTCCGCCAGATTTTGTGTCAATATTTGATGACTTGGCTTCTTCGTCTCTTGATGTCTTCTTTGATCCTTCTCGTGCATTAGTGACTCTTCCAGGTTTGACTTTAGGTACCTTCAAATTAATCAGTAAATGTATCGATGGTAAAGGATTCTTGGGGACAAAACGCTATTTTGGTGACCTAGGAAAATCTTTGAGGACGGCAGGGTCAAATGTATTGTTTGCTGCAGTCACGGAAATATCAGACTCTATTCTGAAAGGAGCAGAAGCAAGTGGTTTTGATGGAGTGGTCACGGGCTTTCACCATGGAATATTGAAATTGGCTATGGAGCCATCATTATTGGGAACTGCTTTGATGGAAGGTGGCCCAGATAGAAAGATTAAGCTTGACCGCAGTCCTGCTGTTGATGAGTTATATATCGAAGGATACTTGCAAGCGATGTTGGATACGATGTATAGACAAGAATATTTGAGAGTCAGAGTCATCGAGAATCAGGTTTACCTCAAAAACCTGCCCCCAAACAGTTCCCTTATAAACGAGATTATGGATCGTGTGAAGGGGTTTCTTGTGAGCAAGTCACTTCTGAAAGGAGACCCCTCAATAACATCTCGCCCCTTGGGTCATCTTCGAGGTGAACGTGAGTGGAGAATCGGCCCAACTGTGCTGACATTAGGTGAGCATCTGTTCGTGAGTTTCGCAATCCGAATCCTGCGGAAGCAAGCTAACAAGTGCATCGCCAACATCAAATGGAAGCCAGAGAGCGATGGTGGTCGATCGATTGTCCCTGCAAGCTCCTCGGAAGAAGTGGTGAAGGGTAAGTTCATTTGGAAATGGGGAAGTGGGATCGGCAAGTTTGTTTTGTCAGCCATAGTAGCATATATAGATGGACGGTTATGTCGTTCTATTCCTAATCCTGTAGCTCGCCGCATCGTTAGTGGCTTTCTGTTGACTTTCCTTGATAACAACAATAGCAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SHR-BD VPS13_C

Protein Analysis

3234

Amino Acids

361.17

Weight (kDa)

6.44

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_VAB PF25036 2154 - 2572 2.5e-19 Vacuolar-sorting associated protein 13, adaptor binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 4998, 8636, 9254
AasI GACNNNNNNGTC 4 cut(s) 3756, 8548, 8926, 9615
AatII GACGTC 2 cut(s) 4764, 6544
Acc16I TGCGCA 1 cut(s) 8665
Acc36I ACCTGC 2 cut(s) 671, 9240
Acc65I GGTACC 1 cut(s) 8831
AccB1I GGYRCC 2 cut(s) 7291, 8831
AccB7I CCANNNNNTGG 3 cut(s) 2431, 2566, 6975
AccBSI CCGCTC 1 cut(s) 38
AccI GTMKAC 4 cut(s) 753, 1551, 6345, 8105
AccII CGCG 3 cut(s) 137, 6002, 8646
AccIII TCCGGA 1 cut(s) 796
AclI AACGTT 1 cut(s) 3930
AcoI YGGCCR 4 cut(s) 33, 47, 2342, 6051
AcyI GRCGYC 3 cut(s) 258, 4761, 6541
AdeI CACNNNGTG 1 cut(s) 109
AfeI AGCGCT 2 cut(s) 4172, 4621
AflII CTTAAG 3 cut(s) 3427, 6778, 7960
AflIII ACRYGT 7 cut(s) 694, 1557, 1809, 2191, 6000, 6514, 7192
AjuI GAANNNNNNNTTGG 8 cut(s) 486, 518, 1967, 1999, 3920, 3952, 4308, 4340
AloI GAACNNNNNNTCC 4 cut(s) 5439, 5471, 6204, 6236
Alw44I GTGCAC 2 cut(s) 2318, 2930
AlwNI CAGNNNCTG 4 cut(s) 2390, 2431, 3542, 5435
Ama87I CYCGRG 5 cut(s) 74, 279, 6789, 7867, 7953
Aor13HI TCCGGA 1 cut(s) 796
Aor51HI AGCGCT 2 cut(s) 4172, 4621
ApaI GGGCCC 1 cut(s) 2382
ApaLI GTGCAC 2 cut(s) 2318, 2930
AseI ATTAAT 1 cut(s) 8843
Asp700I GAANNNNTTC 5 cut(s) 1734, 4339, 4973, 6766, 7808
Asp718I GGTACC 1 cut(s) 8831
AspA2I CCTAGG 1 cut(s) 8902
AspLEI GCGC 9 cut(s) 142, 2851, 3511, 3863, 4173, 4622, 4728, 8646, 8666
AsuC2I CCSGG 4 cut(s) 2073, 3423, 7954, 7955
AsuNHI GCTAGC 1 cut(s) 883
AvaI CYCGRG 5 cut(s) 74, 279, 6789, 7867, 7953
AvrII CCTAGG 1 cut(s) 8902
AxyI CCTNAGG 3 cut(s) 360, 2414, 6098
BaeGI GKGCMC 4 cut(s) 2322, 2382, 2934, 4792
BaeI ACNNNNGTAYC 2 cut(s) 3720, 3753
BalI TGGCCA 2 cut(s) 2344, 6053
BanI GGYRCC 2 cut(s) 7291, 8831
BanII GRGCYC 6 cut(s) 81, 635, 2382, 3903, 4851, 8607
BarI GAAGNNNNNNTAC 6 cut(s) 1557, 1589, 5656, 5688, 7796, 7828
BauI CACGAG 2 cut(s) 3902, 8796
BbsI GAAGAC 4 cut(s) 578, 6003, 7754, 8773
BbvCI CCTCAGC 1 cut(s) 2383
BceAI ACGGC 3 cut(s) 28, 34, 8937
BcgI CGANNNNNNTGC 4 cut(s) 7031, 7065, 9498, 9532
BciVI GTATCC 3 cut(s) 304, 9140, 9160
BclI TGATCA 5 cut(s) 93, 1771, 3160, 4431, 8458
BcnI CCSGG 4 cut(s) 2073, 3423, 7954, 7955
BfoI RGCGCY 3 cut(s) 143, 4174, 4623
BfrI CTTAAG 3 cut(s) 3427, 6778, 7960
BfuAI ACCTGC 2 cut(s) 671, 9240
BfuI GTATCC 3 cut(s) 304, 9140, 9160
BglI GCCNNNNNGGC 1 cut(s) 8693
BglII AGATCT 3 cut(s) 1354, 5759, 7106
BlnI CCTAGG 1 cut(s) 8902
BlpI GCTNAGC 2 cut(s) 3484, 4582
BmcAI AGTACT 4 cut(s) 1865, 3157, 5572, 8342
BmeT110I CYCGRG 5 cut(s) 74, 279, 6789, 7867, 7953
BmrI ACTGGG 3 cut(s) 3467, 4852, 5375
BmtI GCTAGC 1 cut(s) 887
BmuI ACTGGG 3 cut(s) 3467, 4852, 5375
BoxI GACNNNNGTC 1 cut(s) 9114
BpiI GAAGAC 4 cut(s) 578, 6003, 7754, 8773
BplI GAGNNNNNCTC 4 cut(s) 7279, 7311, 8407, 8439
BpmI CTGGAG 5 cut(s) 1291, 2587, 4234, 5700, 7382
Bpu10I CCTNAGC 3 cut(s) 2383, 2475, 3361
Bpu1102I GCTNAGC 2 cut(s) 3484, 4582
BpuEI CTTGAG 4 cut(s) 3929, 4020, 5790, 7457
BpuMI CCSGG 4 cut(s) 2073, 3423, 7954, 7955
Bsa29I ATCGAT 2 cut(s) 8859, 9497
BsaAI YACGTR 1 cut(s) 7772
BsaBI GATNNNNATC 7 cut(s) 1609, 1993, 2441, 4533, 6465, 6570, 8373
BsaHI GRCGYC 3 cut(s) 258, 4761, 6541
BsaI GGTCTC 1 cut(s) 9306
BsaWI WCCGGW 2 cut(s) 796, 4441
BsaXI ACNNNNNCTCC 6 cut(s) 5439, 5469, 5594, 5624, 8198, 8228
Bse21I CCTNAGG 3 cut(s) 360, 2414, 6098
Bse3DI GCAATG 9 cut(s) 1696, 2165, 4721, 5464, 7446, 7525, 8244, 8720, 9700
Bse8I GATNNNNATC 7 cut(s) 1609, 1993, 2441, 4533, 6465, 6570, 8373
BseAI TCCGGA 1 cut(s) 796
BseCI ATCGAT 2 cut(s) 8859, 9497
BseJI GATNNNNATC 7 cut(s) 1609, 1993, 2441, 4533, 6465, 6570, 8373
BseMI GCAATG 9 cut(s) 1696, 2165, 4721, 5464, 7446, 7525, 8244, 8720, 9700
BseRI GAGGAG 5 cut(s) 4439, 5916, 8399, 8552, 9505
BseSI GKGCMC 4 cut(s) 2322, 2382, 2934, 4792
BseX3I CGGCCG 1 cut(s) 47
BsgI GTGCAG 4 cut(s) 739, 1371, 4611, 8543
Bsh1236I CGCG 3 cut(s) 137, 6002, 8646
Bsh1285I CGRYCG 2 cut(s) 50, 9497
BshNI GGYRCC 2 cut(s) 7291, 8831
BshVI ATCGAT 2 cut(s) 8859, 9497
BsiEI CGRYCG 2 cut(s) 50, 9497
BsiHKCI CYCGRG 5 cut(s) 74, 279, 6789, 7867, 7953
BsiSI CCGG 7 cut(s) 22, 68, 797, 2073, 3422, 4442, 7954
BsmBI CGTCTC 5 cut(s) 4768, 6230, 7007, 8381, 8775
BsmI GAATGC 3 cut(s) 821, 4711, 6927
Bso31I GGTCTC 1 cut(s) 9306
BsoBI CYCGRG 5 cut(s) 74, 279, 6789, 7867, 7953
Bsp120I GGGCCC 1 cut(s) 2378
Bsp13I TCCGGA 1 cut(s) 796
Bsp1407I TGTACA 5 cut(s) 3794, 3892, 6391, 8188, 8329
Bsp1720I GCTNAGC 2 cut(s) 3484, 4582
Bsp19I CCATGG 3 cut(s) 6048, 6597, 9025
BspDI ATCGAT 2 cut(s) 8859, 9497
BspEI TCCGGA 1 cut(s) 796
BspFNI CGCG 3 cut(s) 137, 6002, 8646
BspMAI CTGCAG 4 cut(s) 2653, 2746, 8361, 8950
BspMI ACCTGC 2 cut(s) 671, 9240
BspOI GCTAGC 1 cut(s) 887
BspQI GCTCTTC 3 cut(s) 57, 4161, 6444
BspT107I GGYRCC 2 cut(s) 7291, 8831
BspTI CTTAAG 3 cut(s) 3427, 6778, 7960
BspTNI GGTCTC 1 cut(s) 9306
BsrBI CCGCTC 1 cut(s) 38
BsrDI GCAATG 9 cut(s) 1696, 2165, 4721, 5464, 7446, 7525, 8244, 8720, 9700
BsrGI TGTACA 5 cut(s) 3794, 3892, 6391, 8188, 8329
BssNAI GTATAC 1 cut(s) 8106
BssNI GRCGYC 3 cut(s) 258, 4761, 6541
BssSI CACGAG 2 cut(s) 3902, 8796
BssT1I CCWWGG 7 cut(s) 5239, 6048, 6597, 7567, 8902, 9025, 9336
Bst1107I GTATAC 1 cut(s) 8106
Bst2BI CACGAG 2 cut(s) 3902, 8796
BstACI GRCGYC 3 cut(s) 258, 4761, 6541
BstAFI CTTAAG 3 cut(s) 3427, 6778, 7960
BstAPI GCANNNNNTGC 1 cut(s) 3016
BstAUI TGTACA 5 cut(s) 3794, 3892, 6391, 8188, 8329
BstBAI YACGTR 1 cut(s) 7772
BstDSI CCRYGG 5 cut(s) 50, 135, 6048, 6597, 9025
BstEII GGTNACC 1 cut(s) 8897
BstENI CCTNNNNNAGG 1 cut(s) 3992
BstFNI CGCG 3 cut(s) 137, 6002, 8646
BstH2I RGCGCY 3 cut(s) 143, 4174, 4623
BstHHI GCGC 9 cut(s) 142, 2851, 3511, 3863, 4173, 4622, 4728, 8646, 8666
BstMCI CGRYCG 2 cut(s) 50, 9497
BstPAI GACNNNNGTC 1 cut(s) 9114
BstPI GGTNACC 1 cut(s) 8897
BstSLI GKGCMC 4 cut(s) 2322, 2382, 2934, 4792
BstUI CGCG 3 cut(s) 137, 6002, 8646
BstV2I GAAGAC 4 cut(s) 578, 6003, 7754, 8773
BstX2I RGATCY 9 cut(s) 559, 1354, 1604, 2484, 4745, 5759, 7094, 7106, 8469
BstYI RGATCY 9 cut(s) 559, 1354, 1604, 2484, 4745, 5759, 7094, 7106, 8469
BstZ17I GTATAC 1 cut(s) 8106
BstZI CGGCCG 1 cut(s) 47
Bsu15I ATCGAT 2 cut(s) 8859, 9497
Bsu36I CCTNAGG 3 cut(s) 360, 2414, 6098
BsuI GTATCC 3 cut(s) 304, 9140, 9160
BsuTUI ATCGAT 2 cut(s) 8859, 9497
BtgI CCRYGG 5 cut(s) 50, 135, 6048, 6597, 9025
BtgZI GCGATG 5 cut(s) 3643, 5070, 9173, 9439, 9498
BtsI GCAGTG 5 cut(s) 658, 2881, 5257, 6303, 6691
BveI ACCTGC 2 cut(s) 671, 9240
CaiI CAGNNNCTG 4 cut(s) 2390, 2431, 3542, 5435
CfoI GCGC 9 cut(s) 142, 2851, 3511, 3863, 4173, 4622, 4728, 8646, 8666
Cfr42I CCGCGG 1 cut(s) 138
Cfr9I CCCGGG 1 cut(s) 7953
ClaI ATCGAT 2 cut(s) 8859, 9497
CseI GACGC 4 cut(s) 247, 6008, 6543, 6989
CspCI CAANNNNNGTGG 2 cut(s) 7719, 7754
DraI TTTAAA 3 cut(s) 1897, 4311, 8373
DraIII CACNNNGTG 1 cut(s) 109
DrdI GACNNNNNNGTC 4 cut(s) 3756, 8548, 8926, 9615
DseDI GACNNNNNNGTC 4 cut(s) 3756, 8548, 8926, 9615
EaeI YGGCCR 4 cut(s) 33, 47, 2342, 6051
EagI CGGCCG 1 cut(s) 47
EciI GGCGGA 3 cut(s) 40, 5202, 8718
Ecl136II GAGCTC 4 cut(s) 79, 633, 3901, 8605
EclXI CGGCCG 1 cut(s) 47
Eco130I CCWWGG 7 cut(s) 5239, 6048, 6597, 7567, 8902, 9025, 9336
Eco147I AGGCCT 1 cut(s) 597
Eco24I GRGCYC 6 cut(s) 81, 635, 2382, 3903, 4851, 8607
Eco31I GGTCTC 1 cut(s) 9306
Eco32I GATATC 3 cut(s) 1581, 2443, 4689
Eco47III AGCGCT 2 cut(s) 4172, 4621
Eco52I CGGCCG 1 cut(s) 47
Eco53kI GAGCTC 4 cut(s) 79, 633, 3901, 8605
Eco81I CCTNAGG 3 cut(s) 360, 2414, 6098
Eco88I CYCGRG 5 cut(s) 74, 279, 6789, 7867, 7953
Eco91I GGTNACC 1 cut(s) 8897
EcoICRI GAGCTC 4 cut(s) 79, 633, 3901, 8605
EcoNI CCTNNNNNAGG 1 cut(s) 3992
EcoO65I GGTNACC 1 cut(s) 8897
EcoRI GAATTC 2 cut(s) 4660, 7529
EcoRV GATATC 3 cut(s) 1581, 2443, 4689
EcoT14I CCWWGG 7 cut(s) 5239, 6048, 6597, 7567, 8902, 9025, 9336
EcoT22I ATGCAT 3 cut(s) 6354, 7027, 7448
EcoT38I GRGCYC 6 cut(s) 81, 635, 2382, 3903, 4851, 8607
ErhI CCWWGG 7 cut(s) 5239, 6048, 6597, 7567, 8902, 9025, 9336
Esp3I CGTCTC 5 cut(s) 4768, 6230, 7007, 8381, 8775
FalI AAGNNNNNCTT 4 cut(s) 3075, 3107, 4565, 4597
FauI CCCGC 2 cut(s) 2039, 4579
FauNDI CATATG 3 cut(s) 4542, 6037, 8161
FbaI TGATCA 5 cut(s) 93, 1771, 3160, 4431, 8458
FblI GTMKAC 4 cut(s) 753, 1551, 6345, 8105
FriOI GRGCYC 6 cut(s) 81, 635, 2382, 3903, 4851, 8607
FspAI RTGCGCAY 1 cut(s) 8665
FspI TGCGCA 1 cut(s) 8665
GlaI GCGC 9 cut(s) 141, 2850, 3510, 3862, 4172, 4621, 4727, 8645, 8665
GsuI CTGGAG 5 cut(s) 1291, 2587, 4234, 5700, 7382
HaeII RGCGCY 3 cut(s) 143, 4174, 4623
HapII CCGG 7 cut(s) 22, 68, 797, 2073, 3422, 4442, 7954
HgaI GACGC 4 cut(s) 247, 6008, 6543, 6989
HhaI GCGC 9 cut(s) 142, 2851, 3511, 3863, 4173, 4622, 4728, 8646, 8666
Hin1I GRCGYC 3 cut(s) 258, 4761, 6541
Hin6I GCGC 9 cut(s) 140, 2849, 3509, 3861, 4171, 4620, 4726, 8644, 8664
HinP1I GCGC 9 cut(s) 140, 2849, 3509, 3861, 4171, 4620, 4726, 8644, 8664
HincII GTYRAC 7 cut(s) 686, 4759, 5881, 6346, 6966, 8313, 9669
HindII GTYRAC 7 cut(s) 686, 4759, 5881, 6346, 6966, 8313, 9669
HindIII AAGCTT 8 cut(s) 299, 341, 3828, 3968, 5087, 5410, 7118, 9104
HpaII CCGG 7 cut(s) 22, 68, 797, 2073, 3422, 4442, 7954
Hpy99I CGWCG 2 cut(s) 161, 5982
Hsp92I GRCGYC 3 cut(s) 258, 4761, 6541
HspAI GCGC 9 cut(s) 140, 2849, 3509, 3861, 4171, 4620, 4726, 8644, 8664
Kpn2I TCCGGA 1 cut(s) 796
KpnI GGTACC 1 cut(s) 8835
Ksp22I TGATCA 5 cut(s) 93, 1771, 3160, 4431, 8458
KspI CCGCGG 1 cut(s) 138
LguI GCTCTTC 3 cut(s) 57, 4161, 6444
MbiI CCGCTC 1 cut(s) 38
MfeI CAATTG 4 cut(s) 243, 4859, 5587, 7490
MflI RGATCY 9 cut(s) 559, 1354, 1604, 2484, 4745, 5759, 7094, 7106, 8469
MlsI TGGCCA 2 cut(s) 2344, 6053
MluI ACGCGT 1 cut(s) 6000
MluNI TGGCCA 2 cut(s) 2344, 6053
MmeI TCCRAC 7 cut(s) 1797, 2221, 3918, 4306, 5767, 7973, 9144
Mox20I TGGCCA 2 cut(s) 2344, 6053
Mph1103I ATGCAT 3 cut(s) 6354, 7027, 7448
MroI TCCGGA 1 cut(s) 796
MroXI GAANNNNTTC 5 cut(s) 1734, 4339, 4973, 6766, 7808
MscI TGGCCA 2 cut(s) 2344, 6053
MslI CAYNNNNRTG 7 cut(s) 1144, 1440, 2231, 4110, 5006, 6053, 7910
Msp20I TGGCCA 2 cut(s) 2344, 6053
MspA1I CMGCKG 5 cut(s) 137, 944, 1127, 4802, 7303
MspCI CTTAAG 3 cut(s) 3427, 6778, 7960
MspI CCGG 7 cut(s) 22, 68, 797, 2073, 3422, 4442, 7954
MunI CAATTG 4 cut(s) 243, 4859, 5587, 7490
Mva1269I GAATGC 3 cut(s) 821, 4711, 6927
MvnI CGCG 3 cut(s) 137, 6002, 8646
NciI CCSGG 4 cut(s) 2073, 3423, 7954, 7955
NcoI CCATGG 3 cut(s) 6048, 6597, 9025
NdeI CATATG 3 cut(s) 4542, 6037, 8161
NheI GCTAGC 1 cut(s) 883
NmeAIII GCCGAG 1 cut(s) 132
NsbI TGCGCA 1 cut(s) 8665
NsiI ATGCAT 3 cut(s) 6354, 7027, 7448
PaeI GCATGC 2 cut(s) 380, 4700
PaeR7I CTCGAG 2 cut(s) 74, 6789
PceI AGGCCT 1 cut(s) 597
PciI ACATGT 5 cut(s) 1557, 1809, 2191, 6514, 7192
PciSI GCTCTTC 3 cut(s) 57, 4161, 6444
PcsI WCGNNNNNNNCGW 3 cut(s) 5977, 9355, 9616
PctI GAATGC 3 cut(s) 821, 4711, 6927
PdmI GAANNNNTTC 5 cut(s) 1734, 4339, 4973, 6766, 7808
PflMI CCANNNNNTGG 3 cut(s) 2431, 2566, 6975
PfoI TCCNGGA 1 cut(s) 4153
Ple19I CGATCG 1 cut(s) 9497
Ppu21I YACGTR 1 cut(s) 7772
PpuMI RGGWCCY 6 cut(s) 2898, 3997, 4151, 4872, 8550, 8575
PscI ACATGT 5 cut(s) 1557, 1809, 2191, 6514, 7192
PshAI GACNNNNGTC 1 cut(s) 9114
PshBI ATTAAT 1 cut(s) 8843
PsiI TTATAA 3 cut(s) 4998, 8636, 9254
Psp124BI GAGCTC 4 cut(s) 81, 635, 3903, 8607
Psp1406I AACGTT 1 cut(s) 3930
Psp5II RGGWCCY 6 cut(s) 2898, 3997, 4151, 4872, 8550, 8575
PspEI GGTNACC 1 cut(s) 8897
PspOMI GGGCCC 1 cut(s) 2378
PspPPI RGGWCCY 6 cut(s) 2898, 3997, 4151, 4872, 8550, 8575
PspXI VCTCGAGB 1 cut(s) 74
PstI CTGCAG 4 cut(s) 2653, 2746, 8361, 8950
PstNI CAGNNNCTG 4 cut(s) 2390, 2431, 3542, 5435
PsuI RGATCY 9 cut(s) 559, 1354, 1604, 2484, 4745, 5759, 7094, 7106, 8469
PvuI CGATCG 1 cut(s) 9497
PvuII CAGCTG 3 cut(s) 944, 1127, 7303
RseI CAYNNNNRTG 7 cut(s) 1144, 1440, 2231, 4110, 5006, 6053, 7910
SacI GAGCTC 4 cut(s) 81, 635, 3903, 8607
SacII CCGCGG 1 cut(s) 138
SalI GTCGAC 1 cut(s) 6344
SapI GCTCTTC 3 cut(s) 57, 4161, 6444
ScaI AGTACT 4 cut(s) 1865, 3157, 5572, 8342
Sfr274I CTCGAG 2 cut(s) 74, 6789
Sfr303I CCGCGG 1 cut(s) 138
SgrBI CCGCGG 1 cut(s) 138
SlaI CTCGAG 2 cut(s) 74, 6789
SmaI CCCGGG 1 cut(s) 7955
SmiI ATTTAAAT 1 cut(s) 8373
SmiMI CAYNNNNRTG 7 cut(s) 1144, 1440, 2231, 4110, 5006, 6053, 7910
SmlI CTYRAG 9 cut(s) 74, 3427, 3908, 4035, 5805, 6778, 6789, 7472, 7960
SmoI CTYRAG 9 cut(s) 74, 3427, 3908, 4035, 5805, 6778, 6789, 7472, 7960
SphI GCATGC 2 cut(s) 380, 4700
SseBI AGGCCT 1 cut(s) 597
SstI GAGCTC 4 cut(s) 81, 635, 3903, 8607
StuI AGGCCT 1 cut(s) 597
StyI CCWWGG 7 cut(s) 5239, 6048, 6597, 7567, 8902, 9025, 9336
SwaI ATTTAAAT 1 cut(s) 8373
TauI GCSGC 6 cut(s) 38, 49, 140, 2540, 8098, 9648
TspGWI ACGGA 4 cut(s) 1368, 6521, 7843, 8969
TspMI CCCGGG 1 cut(s) 7953
Van91I CCANNNNNTGG 3 cut(s) 2431, 2566, 6975
Vha464I CTTAAG 3 cut(s) 3427, 6778, 7960
VneI GTGCAC 2 cut(s) 2318, 2930
VspI ATTAAT 1 cut(s) 8843
XagI CCTNNNNNAGG 1 cut(s) 3992
XbaI TCTAGA 1 cut(s) 5756
XhoI CTCGAG 2 cut(s) 74, 6789
XmaI CCCGGG 1 cut(s) 7953
XmaJI CCTAGG 1 cut(s) 8902
XmiI GTMKAC 4 cut(s) 753, 1551, 6345, 8105
XmnI GAANNNNTTC 5 cut(s) 1734, 4339, 4973, 6766, 7808
ZraI GACGTC 2 cut(s) 4762, 6542
ZrmI AGTACT 4 cut(s) 1865, 3157, 5572, 8342
Zsp2I ATGCAT 3 cut(s) 6354, 7027, 7448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.