AT3G51860

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002917
Physical Location & Seq
Forward (+)
19239305 .. 19242913
3609 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G51860.1

Sequence Viewer

Length: 1380 bp
ATGGGAAGTATCGTGGAGCCATGGGCAGCAATCGCCGAGAACGGAAACGCAAACGTGACCGCGAAAGGCTCGAGCAGGGAGCTGCGACATGGGAGAACAGCACACAACATGTCTTCATCGTCGCTAAGGAAGAAATCAGACCTGAGATTGGTTCAGAAAGTTCCATGCAAAACTCTCAAGAACATTCTCTCTAATCTTCAAGAAGTCATTCTTGGTACTAAGCTTACTCTCTTATTTCTCGCCATCCCTCTCGCCATTCTTGCCAATTCTTACAACTACGGTCGTCCGTTGATATTTGGACTGAGCTTGATAGGGCTGACACCTCTAGCTGAGCGAGTTAGCTTTTTGACAGAGCAACTAGCTTTCTACACTGGTCCAACAGTGGGCGGTTTGTTGAACGCGACTTGTGGAAACGCGACAGAGCTGATAATCGCGATACTAGCGTTGGCCAATAACAAAGTGGCAGTGGTGAAATACTCTCTATTGGGTTCAATTCTCTCAAACCTTCTCTTGGTTCTTGGCACTTCCCTCTTCTTTGGTGGTATCGCCAATATCCGCCGCGAGCAGCGGTTCGACCGGAAACAAGCCGATGTGAACTTCTTCTTGCTGCTTATGGGCCTGTTGTGTCATTTGCTGCCATTATTGTTAAAATATGCAGCAACCGGCGAAGTATCGACCTCTATGATTAACAAAATGTCGCTCACTCTGTCGCGGACAAGCAGCATAGTTATGCTTATTGCTTACATTGCTTATCTCATCTTCCAGCTCTGGACTCACCGCCAATTGTTTGAGGCACAACAGGATGATGATGATGCATATGATGATGAGGTTAGTGTTGAAGAAACTCCAGTGATAGGATTCTGGAGCGGATTTGCTTGGCTCGTTGGGATGACAATAGTCATCGCATTGCTATCAGAGTATGTTGTGGACACGATCGAGGATGCATCGGACTCATGGGGACTATCAGTGAGTTTCATAAGCATCATATTGCTTCCCATTGTTGGGAATGCGGCTGAGCATGCTGGAGCCATCATTTTCGCATTCAAGAACAAGCTCGACATATCTCTAGGGGTTGCGTTGGGCTCTGCAACTCAGATTTCTTTGTTCGTGGTCCCATTGAGTGTTATCGTTGCGTGGATCCTGGGAATAAAAATGGATCTCAACTTTAACATCCTTGAAACTAGCTCTCTAGCTTTGGCCATTATCATCACAGCCTTCACTTTACAGGATGGAACTTCTCATTACATGAAGGGACTGGTTCTATTGTTATGCTATGTCATCATCGCGGCGTGTTTCTTCGTCGACCAAATTCCCCAACCAAATGATTTGGACGTGGGACTTCAACCCATGAACAATTTGGGAGAAGTTTTCTCAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006793 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0006882 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008324 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009624 GO:0009628 GO:0009631 GO:0009651 GO:0009705 GO:0009719 GO:0009725 GO:0009733 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015075 GO:0015077 GO:0015078 GO:0015085 GO:0015291 GO:0015297 GO:0015298 GO:0015299 GO:0015318 GO:0015368 GO:0015369 GO:0015491 GO:0015672 GO:0016020 GO:0019725 GO:0022622 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030026 GO:0031090 GO:0032501 GO:0032502 GO:0034220 GO:0042221 GO:0042592 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046916 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0050896 GO:0051139 GO:0051179 GO:0051234 GO:0051592 GO:0051704 GO:0051707 GO:0055062 GO:0055065 GO:0055069 GO:0055071 GO:0055074 GO:0055076 GO:0055080 GO:0055081 GO:0055082 GO:0055083 GO:0055085 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0072503 GO:0072505 GO:0072506 GO:0072507 GO:0072511 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098805 GO:0099402 GO:0099516 GO:1902600
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_Ca_ex

Protein Analysis

459

Amino Acids

49.85

Weight (kDa)

5.44

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 97 - 257 1.7e-20 Sodium/calcium exchanger protein
Na_Ca_ex PF01699 290 - 433 5.6e-22 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 867
AccI GTMKAC 1 cut(s) 1302
AccII CGCG 7 cut(s) 62, 401, 416, 434, 561, 712, 1286
AclWI GGATC 3 cut(s) 1132, 1145, 1164
AcoI YGGCCR 2 cut(s) 447, 1197
AcsI RAATTY 1 cut(s) 1308
AfaI GTAC 1 cut(s) 217
AfiI CCNNNNNNNGG 6 cut(s) 148, 383, 511, 854, 1001, 1002
AflIII ACRYGT 1 cut(s) 108
AgsI TTSAA 7 cut(s) 200, 397, 492, 839, 1045, 1178, 1343
AjiI CACGTC 1 cut(s) 1333
AjnI CCWGG 1 cut(s) 1140
AjuI GAANNNNNNNTTGG 2 cut(s) 195, 227
AlwI GGATC 3 cut(s) 1132, 1145, 1164
Ama87I CYCGRG 1 cut(s) 70
AoxI GGCC 3 cut(s) 447, 616, 1197
ApeKI GCWGC 7 cut(s) 26, 82, 565, 607, 634, 656, 720
ApoI RAATTY 1 cut(s) 1308
Asp700I GAANNNNTTC 2 cut(s) 207, 599
AspS9I GGNCC 3 cut(s) 374, 616, 1111
AsuHPI GGTGA 2 cut(s) 481, 767
AvaI CYCGRG 1 cut(s) 70
AvaII GGWCC 2 cut(s) 374, 1111
BaeI ACNNNNGTAYC 2 cut(s) 428, 461
BalI TGGCCA 2 cut(s) 449, 1199
BamHI GGATCC 1 cut(s) 1137
BanII GRGCYC 1 cut(s) 1085
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 7 cut(s) 38, 69, 577, 594, 621, 668, 732
BccI CCATC 3 cut(s) 251, 1037, 1223
BciT130I CCWGG 1 cut(s) 1142
BfaI CTAG 6 cut(s) 326, 359, 440, 1067, 1182, 1190
BlpI GCTNAGC 2 cut(s) 330, 1014
Bme1390I CCNGG 1 cut(s) 1142
Bme18I GGWCC 2 cut(s) 374, 1111
BmeT110I CYCGRG 1 cut(s) 70
BmgBI CACGTC 1 cut(s) 1333
BmgT120I GGNCC 3 cut(s) 374, 616, 1111
BmiI GGNNCC 4 cut(s) 18, 1027, 1113, 1139
BmrFI CCNGG 1 cut(s) 1142
BmsI GCATC 4 cut(s) 802, 931, 953, 990
BoxI GACNNNNGTC 1 cut(s) 896
BpiI GAAGAC 1 cut(s) 105
BpmI CTGGAG 3 cut(s) 831, 883, 1044
Bpu10I CCTNAGC 1 cut(s) 125
Bpu1102I GCTNAGC 2 cut(s) 330, 1014
BpuEI CTTGAG 1 cut(s) 161
BsaJI CCNNGG 2 cut(s) 20, 1141
BsaWI WCCGGW 1 cut(s) 576
Bsc4I CCNNNNNNNGG 6 cut(s) 148, 383, 511, 854, 1001, 1002
Bse118I RCCGGY 1 cut(s) 662
Bse1I ACTGG 3 cut(s) 376, 848, 1260
Bse3DI GCAATG 2 cut(s) 744, 905
BseBI CCWGG 1 cut(s) 1142
BseDI CCNNGG 2 cut(s) 20, 1141
BseGI GGATG 6 cut(s) 243, 808, 894, 946, 1170, 1234
BseLI CCNNNNNNNGG 6 cut(s) 148, 383, 511, 854, 1001, 1002
BseMI GCAATG 2 cut(s) 744, 905
BseMII CTCAG 5 cut(s) 134, 293, 321, 1005, 1106
BseNI ACTGG 3 cut(s) 376, 848, 1260
BseXI GCAGC 7 cut(s) 38, 69, 577, 594, 621, 668, 732
Bsh1236I CGCG 7 cut(s) 62, 401, 416, 434, 561, 712, 1286
Bsh1285I CGRYCG 3 cut(s) 283, 577, 936
BshFI GGCC 3 cut(s) 449, 618, 1199
BsiEI CGRYCG 3 cut(s) 283, 577, 936
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 2 cut(s) 577, 663
BslFI GGGAC 4 cut(s) 972, 1097, 1266, 1350
BslI CCNNNNNNNGG 6 cut(s) 148, 383, 511, 854, 1001, 1002
BsmFI GGGAC 4 cut(s) 972, 1097, 1266, 1350
BsmI GAATGC 2 cut(s) 1012, 1040
BsnI GGCC 3 cut(s) 449, 618, 1199
BsoBI CYCGRG 1 cut(s) 70
Bsp1286I GDGCHC 1 cut(s) 1085
Bsp143I GATC 3 cut(s) 933, 1137, 1156
Bsp1720I GCTNAGC 2 cut(s) 330, 1014
Bsp19I CCATGG 1 cut(s) 20
Bsp68I TCGCGA 1 cut(s) 434
BspANI GGCC 3 cut(s) 449, 618, 1199
BspCNI CTCAG 5 cut(s) 135, 294, 322, 1006, 1105
BspFNI CGCG 7 cut(s) 62, 401, 416, 434, 561, 712, 1286
BspLI GGNNCC 4 cut(s) 18, 1027, 1113, 1139
BspPI GGATC 3 cut(s) 1132, 1145, 1164
BsrBI CCGCTC 1 cut(s) 867
BsrDI GCAATG 2 cut(s) 744, 905
BsrFI RCCGGY 1 cut(s) 662
BsrI ACTGG 3 cut(s) 376, 848, 1260
BssAI RCCGGY 1 cut(s) 662
BssECI CCNNGG 2 cut(s) 20, 1141
BssMI GATC 3 cut(s) 933, 1137, 1156
BssT1I CCWWGG 1 cut(s) 20
Bst2UI CCWGG 1 cut(s) 1142
Bst4CI ACNGT 2 cut(s) 281, 382
Bst6I CTCTTC 1 cut(s) 536
BstC8I GCNNGC 2 cut(s) 563, 1020
BstDEI CTNAG 8 cut(s) 125, 143, 219, 302, 330, 1014, 1092, 1372
BstDSI CCRYGG 1 cut(s) 20
BstF5I GGATG 6 cut(s) 243, 808, 894, 946, 1170, 1234
BstFNI CGCG 7 cut(s) 62, 401, 416, 434, 561, 712, 1286
BstKTI GATC 3 cut(s) 936, 1140, 1159
BstMBI GATC 3 cut(s) 933, 1137, 1156
BstMCI CGRYCG 3 cut(s) 283, 577, 936
BstMWI GCNNNNNNNGC 5 cut(s) 32, 260, 440, 746, 1019
BstNI CCWGG 1 cut(s) 1142
BstNSI RCATGY 2 cut(s) 112, 1022
BstPAI GACNNNNGTC 1 cut(s) 896
BstSCI CCNGG 1 cut(s) 1140
BstUI CGCG 7 cut(s) 62, 401, 416, 434, 561, 712, 1286
BstV1I GCAGC 7 cut(s) 38, 69, 577, 594, 621, 668, 732
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 2 cut(s) 1137, 1156
BstYI RGATCY 2 cut(s) 1137, 1156
BsuRI GGCC 3 cut(s) 449, 618, 1199
BtgI CCRYGG 1 cut(s) 20
BtgZI GCGATG 2 cut(s) 886, 1267
BtrI CACGTC 1 cut(s) 1333
BtsCI GGATG 6 cut(s) 243, 808, 894, 946, 1170, 1234
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 5 cut(s) 369, 387, 471, 855, 972
BtuMI TCGCGA 1 cut(s) 434
Cac8I GCNNGC 2 cut(s) 563, 1020
Cfr10I RCCGGY 1 cut(s) 662
Cfr13I GGNCC 3 cut(s) 374, 616, 1111
Csp6I GTAC 1 cut(s) 216
CviAII CATG 8 cut(s) 21, 89, 109, 165, 954, 1019, 1246, 1348
CviQI GTAC 1 cut(s) 216
DdeI CTNAG 8 cut(s) 125, 143, 219, 302, 330, 1014, 1092, 1372
DpnI GATC 3 cut(s) 935, 1139, 1158
DpnII GATC 3 cut(s) 933, 1137, 1156
EaeI YGGCCR 2 cut(s) 447, 1197
Eam1104I CTCTTC 1 cut(s) 536
EarI CTCTTC 1 cut(s) 536
EciI GGCGGA 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 1085
Eco47I GGWCC 2 cut(s) 374, 1111
Eco88I CYCGRG 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 1140
EcoT14I CCWWGG 1 cut(s) 20
EcoT22I ATGCAT 2 cut(s) 817, 946
EcoT38I GRGCYC 1 cut(s) 1085
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 8 cut(s) 24, 92, 112, 168, 957, 1022, 1249, 1351
FalI AAGNNNNNCTT 2 cut(s) 195, 227
FaqI GGGAC 4 cut(s) 972, 1097, 1266, 1350
FatI CATG 8 cut(s) 20, 88, 108, 164, 953, 1018, 1245, 1347
FauNDI CATATG 1 cut(s) 817
FblI GTMKAC 1 cut(s) 1302
FokI GGATG 6 cut(s) 230, 815, 901, 953, 1157, 1241
FriOI GRGCYC 1 cut(s) 1085
FspBI CTAG 6 cut(s) 326, 359, 440, 1067, 1182, 1190
GsuI CTGGAG 3 cut(s) 831, 883, 1044
HaeIII GGCC 3 cut(s) 449, 618, 1199
HapII CCGG 2 cut(s) 577, 663
Hin1II CATG 8 cut(s) 24, 92, 112, 168, 957, 1022, 1249, 1351
HincII GTYRAC 1 cut(s) 1303
HindII GTYRAC 1 cut(s) 1303
HindIII AAGCTT 1 cut(s) 221
HinfI GANTC 3 cut(s) 772, 858, 950
HpaII CCGG 2 cut(s) 577, 663
HphI GGTGA 2 cut(s) 481, 767
Hpy166II GTNNAC 3 cut(s) 595, 928, 1303
Hpy188I TCNGA 5 cut(s) 139, 156, 916, 949, 1095
Hpy188III TCNNGA 6 cut(s) 178, 200, 433, 769, 862, 1045
Hpy8I GTNNAC 3 cut(s) 595, 928, 1303
Hpy99I CGWCG 2 cut(s) 124, 1304
HpyAV CCTTC 3 cut(s) 515, 1225, 1243
HpyCH4III ACNGT 2 cut(s) 281, 382
HpyCH4IV ACGT 2 cut(s) 54, 1332
HpyCH4V TGCA 5 cut(s) 168, 656, 815, 944, 1088
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 260, 440, 746, 1019
HpyF3I CTNAG 8 cut(s) 125, 143, 219, 302, 330, 1014, 1092, 1372
HpySE526I ACGT 2 cut(s) 54, 1332
Hsp92II CATG 8 cut(s) 24, 92, 112, 168, 957, 1022, 1249, 1351
Kzo9I GATC 3 cut(s) 933, 1137, 1156
LmnI GCTCC 4 cut(s) 16, 79, 864, 1025
Lsp1109I GCAGC 7 cut(s) 38, 69, 577, 594, 621, 668, 732
LweI GCATC 4 cut(s) 802, 931, 953, 990
MaeI CTAG 6 cut(s) 326, 359, 440, 1067, 1182, 1190
MaeII ACGT 2 cut(s) 54, 1332
MaeIII GTNAC 1 cut(s) 55
MalI GATC 3 cut(s) 935, 1139, 1158
MbiI CCGCTC 1 cut(s) 867
MboI GATC 3 cut(s) 933, 1137, 1156
MboII GAAGA 8 cut(s) 105, 142, 188, 523, 592, 751, 851, 1288
MfeI CAATTG 1 cut(s) 782
MflI RGATCY 2 cut(s) 1137, 1156
MhlI GDGCHC 1 cut(s) 1085
MlsI TGGCCA 2 cut(s) 449, 1199
MluCI AATT 5 cut(s) 265, 492, 782, 1308, 1354
MluNI TGGCCA 2 cut(s) 449, 1199
MlyI GAGTC 2 cut(s) 766, 944
MmeI TCCRAC 1 cut(s) 401
MnlI CCTC 7 cut(s) 258, 333, 539, 688, 784, 820, 931
Mox20I TGGCCA 2 cut(s) 449, 1199
Mph1103I ATGCAT 2 cut(s) 817, 946
MroXI GAANNNNTTC 2 cut(s) 207, 599
MscI TGGCCA 2 cut(s) 449, 1199
MseI TTAA 4 cut(s) 647, 687, 1167, 1378
MslI CAYNNNNRTG 1 cut(s) 728
Msp20I TGGCCA 2 cut(s) 449, 1199
MspA1I CMGCKG 1 cut(s) 568
MspI CCGG 2 cut(s) 577, 663
MspR9I CCNGG 1 cut(s) 1142
MunI CAATTG 1 cut(s) 782
Mva1269I GAATGC 2 cut(s) 1012, 1040
MvaI CCWGG 1 cut(s) 1142
MvnI CGCG 7 cut(s) 62, 401, 416, 434, 561, 712, 1286
MwoI GCNNNNNNNGC 5 cut(s) 32, 260, 440, 746, 1019
NcoI CCATGG 1 cut(s) 20
NdeI CATATG 1 cut(s) 817
NdeII GATC 3 cut(s) 933, 1137, 1156
NlaIII CATG 8 cut(s) 24, 92, 112, 168, 957, 1022, 1249, 1351
NlaIV GGNNCC 4 cut(s) 18, 1027, 1113, 1139
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 55
NruI TCGCGA 1 cut(s) 434
NsiI ATGCAT 2 cut(s) 817, 946
NspI RCATGY 2 cut(s) 112, 1022
PaeI GCATGC 1 cut(s) 1022
PaeR7I CTCGAG 1 cut(s) 70
PciI ACATGT 1 cut(s) 108
PctI GAATGC 2 cut(s) 1012, 1040
PdmI GAANNNNTTC 2 cut(s) 207, 599
PfeI GAWTC 1 cut(s) 858
Ple19I CGATCG 1 cut(s) 936
PleI GAGTC 2 cut(s) 766, 944
PpsI GAGTC 2 cut(s) 766, 944
PscI ACATGT 1 cut(s) 108
PshAI GACNNNNGTC 1 cut(s) 896
Psp6I CCWGG 1 cut(s) 1140
PspGI CCWGG 1 cut(s) 1140
PspN4I GGNNCC 4 cut(s) 18, 1027, 1113, 1139
PspPI GGNCC 3 cut(s) 374, 616, 1111
PspXI VCTCGAGB 1 cut(s) 70
PsuI RGATCY 2 cut(s) 1137, 1156
PvuI CGATCG 1 cut(s) 936
RruI TCGCGA 1 cut(s) 434
RsaI GTAC 1 cut(s) 217
RsaNI GTAC 1 cut(s) 216
RseI CAYNNNNRTG 1 cut(s) 728
SalI GTCGAC 1 cut(s) 1301
SaqAI TTAA 4 cut(s) 647, 687, 1167, 1378
Sau3AI GATC 3 cut(s) 933, 1137, 1156
Sau96I GGNCC 3 cut(s) 374, 616, 1111
SchI GAGTC 2 cut(s) 766, 944
ScrFI CCNGG 1 cut(s) 1142
SduI GDGCHC 1 cut(s) 1085
SfaNI GCATC 4 cut(s) 802, 931, 953, 990
Sfr274I CTCGAG 1 cut(s) 70
SinI GGWCC 2 cut(s) 374, 1111
SlaI CTCGAG 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 728
SmlI CTYRAG 2 cut(s) 70, 176
SmoI CTYRAG 2 cut(s) 70, 176
SphI GCATGC 1 cut(s) 1022
Sse9I AATT 5 cut(s) 265, 492, 782, 1308, 1354
SspMI CTAG 6 cut(s) 326, 359, 440, 1067, 1182, 1190
StyD4I CCNGG 1 cut(s) 1140
StyI CCWWGG 1 cut(s) 20
TaaI ACNGT 2 cut(s) 281, 382
TaiI ACGT 2 cut(s) 57, 1335
TaqI TCGA 6 cut(s) 71, 573, 674, 936, 1056, 1302
TasI AATT 5 cut(s) 265, 492, 782, 1308, 1354
TauI GCSGC 3 cut(s) 561, 1013, 1289
TfiI GAWTC 1 cut(s) 858
Tru1I TTAA 4 cut(s) 647, 687, 1167, 1378
Tru9I TTAA 4 cut(s) 647, 687, 1167, 1378
TscAI CASTG 5 cut(s) 376, 387, 471, 855, 972
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 7 cut(s) 26, 82, 565, 607, 634, 656, 720
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 4 cut(s) 105, 964, 1262, 1364
TspGWI ACGGA 2 cut(s) 57, 276
TspRI CASTG 5 cut(s) 376, 387, 471, 855, 972
VpaK11BI GGWCC 2 cut(s) 374, 1111
XapI RAATTY 1 cut(s) 1308
XceI RCATGY 2 cut(s) 112, 1022
XcmI CCANNNNNNNNNTGG 2 cut(s) 457, 1354
XhoI CTCGAG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 1302
XmnI GAANNNNTTC 2 cut(s) 207, 599
XspI CTAG 6 cut(s) 326, 359, 440, 1067, 1182, 1190
Zsp2I ATGCAT 2 cut(s) 817, 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.