AT3G57300

DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005736
Physical Location & Seq
Forward (+)
21199150 .. 21207893
8744 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G57300.2

Sequence Viewer

Length: 4524 bp
ATGGATCCTTCAAGACGACCACCGAAGGACTCTCCTTACGCGAATCTATTCGATCTCGAGCCGTTGATGAAGTTTAGAATTCCGAAACCTGAAGATGAAGTTGATTATTATGGGAGTAGTAGCCAGGATGAAAGTAGAAGCACTCAAGGTGGGGTAGTGGCAAACTACAGCAATGGGTCTAAATCGAGAATGAATGCGAGCTCCAAGAAGAGAAAGCGGTGGACAGAAGCTGAGGATGCAGAGGACGATGATGATCTCTACAATCAACATGTTACTGAGGAGCACTACCGATCAATGCTTGGGGAGCATGTACAAAAATTCAAAAATAGGTCCAAGGAGACTCAAGGGAATCCTCCTCATCTGATGGGTTTTCCGGTGCTAAAGAGCAATGTGGGCAGTTACAGAGGTAGGAAACCAGGGAATGATTACCATGGGAGGTTCTATGACATGGACAACTCTCCAAATTTTGCAGCTGATGTGACCCCACATAGGCGAGGAAGCTACCATGATCGTGATATTACACCCAAGATAGCATATGAACCTTCGTATTTGGACATTGGTGATGGTGTCATCTACAAAATCCCCCCAAGTTATGACAAGCTGGTGGCATCATTAAACTTACCGAGCTTTTCAGACATTCATGTGGAAGAATTTTACTTGAAAGGAACTCTGGATCTGAGATCATTAGCAGAACTGATGGCAAGTGATAAAAGGTCTGGAGTAAGAAGCCGTAATGGAATGGGTGAGCCTCGACCTCAATATGAATCTCTTCAAGCTAGAATGAAGGCCCTGTCACCTTCAAACTCCACCCCAAATTTTAGCCTCAAGGTGTCAGAAGCTGCAATGAATTCTGCCATTCCAGAAGGATCTGCTGGAAGTACTGCACGGACAATTCTGTCTGAGGGTGGTGTTTTACAGGTCCATTACGTGAAGATTCTGGAGAAGGGGGATACATACGAGATTGTTAAACGAAGTCTACCGAAGAAGCTGAAAGCAAAGAATGATCCTGCAGTCATTGAGAAAACAGAAAGGGATAAAATTAGAAAAGCCTGGATCAATATTGTCAGAAGAGATATAGCAAAACACCATAGAATTTTCACTACTTTTCATCGTAAACTATCAATTGATGCCAAGAGGTTTGCAGATGGTTGCCAAAGAGAGGTGAGAATGAAGGTGGGTAGATCATACAAAATCCCAAGAACTGCACCAATTCGCACTAGGAAGATATCCAGAGACATGCTGCTATTCTGGAAGCGATATGACAAGCAGATGGCAGAAGAGAGGAAAAAGCAAGAAAAGGAAGCTGCAGAGGCTTTTAAACGTGAACAGGAGCAGCGAGAGTCAAAAAGGCAGCAACAAAGGCTCAATTTCCTTATTAAACAGACTGAGCTTTACAGTCACTTCATGCAAAACAAGACCGATTCGAATCCTTCCGAAGCCTTACCAATAGGTGATGAAAATCCGATTGACGAAGTGCTCCCAGAAACTTCAGCGGCAGAACCTTCTGAGGTAGAGGATCCTGAAGAGGCTGAACTGAAGGAAAAGGTCTTGAGAGCTGCCCAAGATGCGGTGTCTAAGCAGAAGCAAATAACAGATGCATTTGACACTGAATATATGAAGCTACGCCAAACTTCTGAAATGGAAGGTCCTTTAAATGATATATCAGTTTCTGGCTCGAGCAATATAGATTTGCATAACCCATCTACAATGCCTGTTACATCAACAGTTCAGACTCCAGAGTTATTTAAAGGAACCCTTAAAGAATACCAAATGAAAGGCCTTCAGTGGCTAGTCAATTGTTATGAGCAGGGTTTGAATGGCATACTTGCTGATGAAATGGGCTTGGGTAAGACTATTCAAGCTATGGCGTTCTTGGCACATTTGGCTGAGGAAAAGAACATTTGGGGTCCATTTCTTGTTGTTGCCCCTGCCTCTGTTCTTAACAATTGGGCTGATGAAATCAGTCGTTTCTGTCCTGACTTGAAAACTCTTCCATATTGGGGAGGATTACAAGAACGAACAATTTTAAGAAAGAATATCAATCCCAAGCGTATGTACCGAAGGGATGCTGGCTTTCATATTTTGATTACTAGCTATCAGCTATTAGTCACTGATGAAAAGTATTTTCGCCGGGTGAAGTGGCAATATATGGTGCTAGATGAGGCCCAAGCAATCAAGAGTTCCTCCAGTATAAGATGGAAAACCCTTCTTAGTTTTAACTGTCGGAACCGATTGCTTCTGACTGGTACTCCAATTCAGAACAACATGGCAGAGTTATGGGCCCTGCTGCATTTCATCATGCCAATGTTGTTTGACAACCATGATCAATTTAATGAATGGTTCTCAAAAGGAATTGAGAATCATGCTGAACACGGAGGCACTTTAAATGAGCACCAGCTTAACAGACTGCATGCGATCTTGAAACCGTTCATGCTTCGACGGGTAAAAAAGGATGTGGTTTCTGAGCTAACTACAAAGACGGAAGTTACAGTACACTGCAAGCTCAGTTCTCGACAACAAGCTTTTTATCAGGCTATTAAGAACAAAATTTCTCTGGCTGAGTTGTTTGATAGCAACCGCGGACAATTTACTGATAAGAAAGTATTGAATTTAATGAATATTGTCATTCAACTAAGGAAGGTTTGCAACCATCCAGAGTTGTTCGAAAGGAATGAAGGGAGCTCGTATCTCTACTTTGGAGTGACTTCCAATTCTCTTTTGCCCCATCCCTTTGGTGAGCTAGAGGATGTACATTATTCTGGTGGTCAAAATCCGATAATATACAAGATACCTAAGCTACTACACCAAGAGGTGCTCCAAAATTCTGAAACATTTTGTTCTTCTGTCGGGCGTGGCATCTCAAGAGAATCTTTTCTGAAGCATTTTAATATATATTCACCTGAGTATATTCTTAAGTCAATATTCCCATCTGATAGTGGGGTAGATCAAGTGGTTAGTGGAAGTGGAGCATTTGGCTTTTCACGCTTGATGGATCTATCACCATCAGAAGTTGGATATCTGGCTCTGTGTTCTGTTGCAGAAAGGCTATTATTTTCTATACTGAGGTGGGAGCGGCAATTTTTGGATGAATTAGTTAACTCTCTTATGGAGTCCAAGGATGGTGATCTTAGTGACAATAACATCGAGAGAGTTAAAACCAAAGCTGTCACAAGAATGTTGCTGATGCCATCAAAAGTTGAAACGAATTTTCAGAAAAGGAGACTAAGCACAGGGCCTACCCGTCCTTCATTTGAAGCGCTAGTGATCTCTCATCAGGATAGGTTTCTTTCAAGTATCAAACTCCTGCATTCTGCATATACTTATATCCCAAAAGCCAGAGCTCCACCTGTAAGCATTCATTGCTCGGACAGAAATTCGGCATACAGAGTTACAGAAGAATTACATCAACCATGGCTTAAGAGACTATTAATCGGTTTTGCACGAACGTCAGAAGCTAATGGACCCAGGAAGCCTAACAGCTTTCCACATCCTTTAATCCAAGAAATTGATTCAGAACTTCCAGTTGTGCAGCCTGCGCTTCAACTGACACACAGAATATTTGGTTCTTGCCCTCCAATGCAAAGTTTTGACCCAGCAAAGTTGCTCACGGACTCTGGGAAGCTGCAGACACTTGATATATTATTGAAGCGGCTTCGAGCTGGAAATCACAGGGTGCTCCTGTTTGCACAAATGACAAAGATGCTGAACATTCTCGAGGATTATATGAACTATAGAAAGTACAAGTACCTCAGGCTTGATGGATCCTCCACCATCATGGATCGCCGAGATATGGTTAGGGATTTTCAGCATAGGAGCGATATTTTTGTATTCTTGCTGAGCACCAGAGCTGGAGGACTTGGTATCAACTTGACGGCTGCAGACACTGTCATTTTCTATGAAAGTGATTGGAATCCCACCTTGGATTTACAAGCTATGGACAGGGCTCATCGTCTTGGACAGACAAAAGATGTTACTGTTTATCGTCTCATCTGTAAGGAGACGGTGGAAGAGAAAATTTTGCACAGGGCAAGTCAGAAAAATACAGTTCAACAGCTTGTTATGACTGGAGGGCATGTTCAGGGTGATGATTTTCTTGGAGCTGCGGATGTGGTATCTCTGCTAATGGATGATGCGGAGGCAGCACAACTGGAGCAGAAATTCAGAGAACTACCATTACAGGTAAAGGACAGGCAGAAGAAAAAGACGAAACGTATCAGAATAGATGCTGAAGGAGATGCAACTTTGGAAGAGTTAGAAGATGTTGACCGACAGGATAACGGACAGGAACCTTTGGAAGAACCGGAAAAGCCAAAATCCAGTAATAAAAAGAGGAGAGCTGCTTCAAATCCGAAAGCTAGAGCTCCTCAGAAAGCAAAGGAAGAAGCAAATGGTGAAGATACTCCTCAGAGGACAAAAAGGGTAAAGAGACAAACAAAGAGCATAAACGAAAGTCTTGAACCTGTATTCTCTGCCTCTGTAACAGAATCAAATAAAGGATTCGATCCAAGTAGCTCCGCTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBINO Helicase_C SNF2_N

Protein Analysis

1507

Amino Acids

172.17

Weight (kDa)

8.97

Isoelectric Point (pI)

49.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBINO PF13892 345 - 475 9.1e-45 DNA-binding domain
ResIII PF04851 585 - 785 2e-06 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 589 - 920 3.2e-84 SNF2-related domain
Helicase_C PF00271 1242 - 1352 6.3e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 895
AccBSI CCGCTC 1 cut(s) 3073
AccI GTMKAC 1 cut(s) 976
AccII CGCG 2 cut(s) 41, 2580
AcuI CTGAAG 7 cut(s) 111, 1473, 1542, 1556, 1766, 2897, 4248
AdeI CACNNNGTG 1 cut(s) 3673
AfaI GTAC 8 cut(s) 312, 880, 2057, 2248, 2493, 2751, 3740, 3746
AfeI AGCGCT 1 cut(s) 3258
AfiI CCNNNNNNNGG 5 cut(s) 489, 1159, 1567, 2000, 3790
AflII CTTAAG 2 cut(s) 2912, 3416
AflIII ACRYGT 1 cut(s) 268
AjnI CCWGG 4 cut(s) 123, 415, 1049, 3464
AjuI GAANNNNNNNTTGG 2 cut(s) 3902, 3934
AloI GAACNNNNNNTCC 2 cut(s) 4059, 4091
Alw26I GTCTC 7 cut(s) 332, 1227, 3214, 3415, 3989, 3992, 4420
AlwNI CAGNNNCTG 4 cut(s) 230, 839, 1670, 3884
Ama87I CYCGRG 3 cut(s) 56, 1675, 3713
Aor51HI AGCGCT 1 cut(s) 3258
AoxI GGCC 5 cut(s) 786, 1777, 2163, 2280, 3233
ApaI GGGCCC 1 cut(s) 2284
AseI ATTAAT 1 cut(s) 3428
Asp700I GAANNNNTTC 4 cut(s) 47, 768, 2426, 2871
AspLEI GCGC 2 cut(s) 3259, 3538
AsuC2I CCSGG 1 cut(s) 2132
AsuII TTCGAA 2 cut(s) 1424, 2664
AvaI CYCGRG 3 cut(s) 56, 1675, 3713
AvaII GGWCC 5 cut(s) 330, 919, 1646, 1907, 3461
AxyI CCTNAGG 1 cut(s) 3749
BaeGI GKGCMC 1 cut(s) 2284
BamHI GGATCC 3 cut(s) 4, 1516, 3761
BanII GRGCYC 6 cut(s) 203, 2284, 2684, 3343, 3946, 4363
BarI GAAGNNNNNNTAC 2 cut(s) 2475, 2507
BbvCI CCTCAGC 2 cut(s) 231, 1887
BceAI ACGGC 3 cut(s) 46, 714, 3888
BciT130I CCWGG 4 cut(s) 125, 417, 1051, 3466
BciVI GTATCC 1 cut(s) 943
BclI TGATCA 1 cut(s) 2323
BcnI CCSGG 1 cut(s) 2132
BcoDI GTCTC 7 cut(s) 332, 1227, 3214, 3415, 3989, 3992, 4420
BfaI CTAG 8 cut(s) 777, 1218, 1790, 2091, 2156, 2741, 3260, 4356
BfmI CTRYAG 6 cut(s) 166, 1008, 1305, 3623, 3730, 3876
BfoI RGCGCY 1 cut(s) 3260
BfrI CTTAAG 2 cut(s) 2912, 3416
BfuI GTATCC 1 cut(s) 943
BlpI GCTNAGC 1 cut(s) 3836
BmcAI AGTACT 1 cut(s) 880
Bme1390I CCNGG 5 cut(s) 125, 417, 1051, 2132, 3466
Bme18I GGWCC 5 cut(s) 330, 919, 1646, 1907, 3461
BmeT110I CYCGRG 3 cut(s) 56, 1675, 3713
BmiI GGNNCC 9 cut(s) 6, 1518, 1753, 1908, 2228, 2282, 3463, 3763, 4287
BmrFI CCNGG 5 cut(s) 125, 417, 1051, 2132, 3466
BpmI CTGGAG 7 cut(s) 738, 959, 1719, 2170, 3870, 4086, 4169
Bpu10I CCTNAGC 3 cut(s) 231, 1887, 2793
Bpu1102I GCTNAGC 1 cut(s) 3836
Bpu14I TTCGAA 2 cut(s) 1424, 2664
BpuEI CTTGAG 5 cut(s) 129, 327, 809, 1570, 2845
BpuMI CCSGG 1 cut(s) 2132
BsaAI YACGTR 1 cut(s) 928
BsaBI GATNNNNATC 5 cut(s) 252, 1425, 1458, 3123, 3909
BsaJI CCNNGG 8 cut(s) 333, 416, 430, 2578, 3114, 3410, 3464, 3918
BsaWI WCCGGW 2 cut(s) 373, 4300
BsaXI ACNNNNNCTCC 4 cut(s) 2233, 2263, 4059, 4089
Bsc4I CCNNNNNNNGG 5 cut(s) 489, 1159, 1567, 2000, 3790
Bse1I ACTGG 6 cut(s) 2187, 2248, 3521, 4069, 4152, 4317
Bse21I CCTNAGG 1 cut(s) 3749
Bse3DI GCAATG 4 cut(s) 178, 394, 849, 3358
Bse8I GATNNNNATC 5 cut(s) 252, 1425, 1458, 3123, 3909
BseBI CCWGG 4 cut(s) 125, 417, 1051, 3466
BseDI CCNNGG 8 cut(s) 333, 416, 430, 2578, 3114, 3410, 3464, 3918
BseJI GATNNNNATC 5 cut(s) 252, 1425, 1458, 3123, 3909
BseLI CCNNNNNNNGG 5 cut(s) 489, 1159, 1567, 2000, 3790
BseMI GCAATG 4 cut(s) 178, 394, 849, 3358
BseNI ACTGG 6 cut(s) 2187, 2248, 3521, 4069, 4152, 4317
BseRI GAGGAG 5 cut(s) 293, 345, 4345, 4353, 4392
BseSI GKGCMC 1 cut(s) 2284
BseYI CCCAGC 1 cut(s) 3592
BsgI GTGCAG 3 cut(s) 867, 1188, 3548
Bsh1236I CGCG 2 cut(s) 41, 2580
BshFI GGCC 5 cut(s) 788, 1779, 2165, 2282, 3235
BsiHKCI CYCGRG 3 cut(s) 56, 1675, 3713
BsiSI CCGG 3 cut(s) 374, 2131, 4301
BslI CCNNNNNNNGG 5 cut(s) 489, 1159, 1567, 2000, 3790
BsmAI GTCTC 7 cut(s) 332, 1227, 3214, 3415, 3989, 3992, 4420
BsmBI CGTCTC 2 cut(s) 3989, 3992
BsmI GAATGC 3 cut(s) 199, 3307, 3354
BsnI GGCC 5 cut(s) 788, 1779, 2165, 2282, 3235
BsoBI CYCGRG 3 cut(s) 56, 1675, 3713
Bsp119I TTCGAA 2 cut(s) 1424, 2664
Bsp120I GGGCCC 1 cut(s) 2280
Bsp1407I TGTACA 2 cut(s) 310, 2749
Bsp1720I GCTNAGC 1 cut(s) 3836
Bsp19I CCATGG 2 cut(s) 430, 3410
BspANI GGCC 5 cut(s) 788, 1779, 2165, 2282, 3235
BspFNI CGCG 2 cut(s) 41, 2580
BspLI GGNNCC 9 cut(s) 6, 1518, 1753, 1908, 2228, 2282, 3463, 3763, 4287
BspMAI CTGCAG 4 cut(s) 1012, 1309, 3627, 3880
BspT104I TTCGAA 2 cut(s) 1424, 2664
BspTI CTTAAG 2 cut(s) 2912, 3416
BsrBI CCGCTC 1 cut(s) 3073
BsrDI GCAATG 4 cut(s) 178, 394, 849, 3358
BsrGI TGTACA 2 cut(s) 310, 2749
BsrI ACTGG 6 cut(s) 2187, 2248, 3521, 4069, 4152, 4317
BssECI CCNNGG 8 cut(s) 333, 416, 430, 2578, 3114, 3410, 3464, 3918
BssT1I CCWWGG 5 cut(s) 333, 430, 3114, 3410, 3918
Bst2UI CCWGG 4 cut(s) 125, 417, 1051, 3466
Bst4CI ACNGT 9 cut(s) 1397, 1726, 2222, 2427, 2491, 3886, 3976, 4003, 4045
Bst6I CTCTTC 8 cut(s) 203, 774, 1063, 1272, 1518, 1995, 4002, 4242
BstAFI CTTAAG 2 cut(s) 2912, 3416
BstAPI GCANNNNNTGC 1 cut(s) 3360
BstAUI TGTACA 2 cut(s) 310, 2749
BstBAI YACGTR 1 cut(s) 928
BstBI TTCGAA 2 cut(s) 1424, 2664
BstC8I GCNNGC 5 cut(s) 199, 2071, 2412, 2501, 3534
BstDSI CCRYGG 3 cut(s) 430, 2578, 3410
BstFNI CGCG 2 cut(s) 41, 2580
BstH2I RGCGCY 1 cut(s) 3260
BstHHI GCGC 2 cut(s) 3259, 3538
BstMAI GTCTC 7 cut(s) 332, 1227, 3214, 3415, 3989, 3992, 4420
BstNI CCWGG 4 cut(s) 125, 417, 1051, 3466
BstNSI RCATGY 5 cut(s) 272, 311, 1240, 2414, 4076
BstSCI CCNGG 5 cut(s) 123, 415, 1049, 2130, 3464
BstSFI CTRYAG 6 cut(s) 166, 1008, 1305, 3623, 3730, 3876
BstSLI GKGCMC 1 cut(s) 2284
BstUI CGCG 2 cut(s) 41, 2580
BstX2I RGATCY 6 cut(s) 4, 673, 866, 1516, 2992, 3761
BstXI CCANNNNNNTGG 2 cut(s) 2732, 3775
BstYI RGATCY 6 cut(s) 4, 673, 866, 1516, 2992, 3761
Bsu36I CCTNAGG 1 cut(s) 3749
BsuI GTATCC 1 cut(s) 943
BsuRI GGCC 5 cut(s) 788, 1779, 2165, 2282, 3235
BtgI CCRYGG 3 cut(s) 430, 2578, 3410
BtsI GCAGTG 1 cut(s) 2494
BtsIMutI CAGTG 5 cut(s) 1605, 1790, 2109, 2494, 3882
Cac8I GCNNGC 5 cut(s) 199, 2071, 2412, 2501, 3534
CaiI CAGNNNCTG 4 cut(s) 230, 839, 1670, 3884
CfoI GCGC 2 cut(s) 3259, 3538
Cfr42I CCGCGG 1 cut(s) 2581
Csp6I GTAC 8 cut(s) 311, 879, 2056, 2247, 2492, 2750, 3739, 3745
CviQI GTAC 8 cut(s) 311, 879, 2056, 2247, 2492, 2750, 3739, 3745
DraI TTTAAA 4 cut(s) 1318, 1653, 1747, 2385
DraIII CACNNNGTG 1 cut(s) 3673
DrdI GACNNNNNNGTC 1 cut(s) 895
DseDI GACNNNNNNGTC 1 cut(s) 895
Eam1104I CTCTTC 8 cut(s) 203, 774, 1063, 1272, 1518, 1995, 4002, 4242
EarI CTCTTC 8 cut(s) 203, 774, 1063, 1272, 1518, 1995, 4002, 4242
Ecl136II GAGCTC 4 cut(s) 201, 2682, 3341, 4361
Eco130I CCWWGG 5 cut(s) 333, 430, 3114, 3410, 3918
Eco147I AGGCCT 1 cut(s) 1779
Eco24I GRGCYC 6 cut(s) 203, 2284, 2684, 3343, 3946, 4363
Eco32I GATATC 2 cut(s) 1227, 3017
Eco47I GGWCC 5 cut(s) 330, 919, 1646, 1907, 3461
Eco47III AGCGCT 1 cut(s) 3258
Eco53kI GAGCTC 4 cut(s) 201, 2682, 3341, 4361
Eco57I CTGAAG 7 cut(s) 111, 1473, 1542, 1556, 1766, 2897, 4248
Eco81I CCTNAGG 1 cut(s) 3749
Eco88I CYCGRG 3 cut(s) 56, 1675, 3713
EcoICRI GAGCTC 4 cut(s) 201, 2682, 3341, 4361
EcoO109I RGGNCCY 4 cut(s) 787, 1646, 2281, 3233
EcoRI GAATTC 2 cut(s) 78, 847
EcoRII CCWGG 4 cut(s) 123, 415, 1049, 3464
EcoRV GATATC 2 cut(s) 1227, 3017
EcoT14I CCWWGG 5 cut(s) 333, 430, 3114, 3410, 3918
EcoT22I ATGCAT 1 cut(s) 1600
EcoT38I GRGCYC 6 cut(s) 203, 2284, 2684, 3343, 3946, 4363
ErhI CCWWGG 5 cut(s) 333, 430, 3114, 3410, 3918
Esp3I CGTCTC 2 cut(s) 3989, 3992
FalI AAGNNNNNCTT 4 cut(s) 1740, 1772, 2854, 2886
FauNDI CATATG 1 cut(s) 535
FbaI TGATCA 1 cut(s) 2323
FblI GTMKAC 1 cut(s) 976
FriOI GRGCYC 6 cut(s) 203, 2284, 2684, 3343, 3946, 4363
FspBI CTAG 8 cut(s) 777, 1218, 1790, 2091, 2156, 2741, 3260, 4356
GlaI GCGC 2 cut(s) 3258, 3537
GsaI CCCAGC 1 cut(s) 3596
GsuI CTGGAG 7 cut(s) 738, 959, 1719, 2170, 3870, 4086, 4169
HaeII RGCGCY 1 cut(s) 3260
HaeIII GGCC 5 cut(s) 788, 1779, 2165, 2282, 3235
HapII CCGG 3 cut(s) 374, 2131, 4301
HhaI GCGC 2 cut(s) 3259, 3538
Hin6I GCGC 2 cut(s) 3257, 3536
HinP1I GCGC 2 cut(s) 3257, 3536
HincII GTYRAC 2 cut(s) 3097, 4264
HindII GTYRAC 2 cut(s) 3097, 4264
HindIII AAGCTT 1 cut(s) 2520
HpaI GTTAAC 1 cut(s) 3097
HpaII CCGG 3 cut(s) 374, 2131, 4301
Hpy166II GTNNAC 7 cut(s) 222, 977, 1115, 1325, 2494, 3097, 4264
Hpy8I GTNNAC 7 cut(s) 222, 977, 1115, 1325, 2494, 3097, 4264
Hpy99I CGWCG 1 cut(s) 2442
HpyCH4III ACNGT 9 cut(s) 1397, 1726, 2222, 2427, 2491, 3886, 3976, 4003, 4045
HpyCH4IV ACGT 4 cut(s) 927, 1321, 3446, 4210
HpySE526I ACGT 4 cut(s) 927, 1321, 3446, 4210
HspAI GCGC 2 cut(s) 3257, 3536
Ksp22I TGATCA 1 cut(s) 2323
KspAI GTTAAC 1 cut(s) 3097
KspI CCGCGG 1 cut(s) 2581
MaeI CTAG 8 cut(s) 777, 1218, 1790, 2091, 2156, 2741, 3260, 4356
MaeII ACGT 4 cut(s) 927, 1321, 3446, 4210
MbiI CCGCTC 1 cut(s) 3073
MfeI CAATTG 3 cut(s) 1122, 1795, 1945
MflI RGATCY 6 cut(s) 4, 673, 866, 1516, 2992, 3761
MlyI GAGTC 6 cut(s) 23, 334, 1349, 1726, 3119, 3605
MmeI TCCRAC 2 cut(s) 2204, 2992
Mph1103I ATGCAT 1 cut(s) 1600
MroXI GAANNNNTTC 4 cut(s) 47, 768, 2426, 2871
MslI CAYNNNNRTG 5 cut(s) 510, 641, 2303, 3173, 3773
MspA1I CMGCKG 3 cut(s) 473, 1493, 2580
MspCI CTTAAG 2 cut(s) 2912, 3416
MspI CCGG 3 cut(s) 374, 2131, 4301
MspR9I CCNGG 5 cut(s) 125, 417, 1051, 2132, 3466
MunI CAATTG 3 cut(s) 1122, 1795, 1945
Mva1269I GAATGC 3 cut(s) 199, 3307, 3354
MvaI CCWGG 4 cut(s) 125, 417, 1051, 3466
MvnI CGCG 2 cut(s) 41, 2580
NciI CCSGG 1 cut(s) 2132
NcoI CCATGG 2 cut(s) 430, 3410
NdeI CATATG 1 cut(s) 535
NlaIV GGNNCC 9 cut(s) 6, 1518, 1753, 1908, 2228, 2282, 3463, 3763, 4287
NmeAIII GCCGAG 1 cut(s) 3809
NmuCI GTSAC 7 cut(s) 478, 792, 1397, 2107, 2701, 3131, 3166
NsiI ATGCAT 1 cut(s) 1600
NspI RCATGY 5 cut(s) 272, 311, 1240, 2414, 4076
NspV TTCGAA 2 cut(s) 1424, 2664
PaeI GCATGC 1 cut(s) 2414
PaeR7I CTCGAG 3 cut(s) 56, 1675, 3713
PceI AGGCCT 1 cut(s) 1779
PciI ACATGT 1 cut(s) 268
PctI GAATGC 3 cut(s) 199, 3307, 3354
PdmI GAANNNNTTC 4 cut(s) 47, 768, 2426, 2871
PflFI GACNNNGTC 1 cut(s) 3884
PleI GAGTC 6 cut(s) 23, 334, 1348, 1726, 3118, 3605
PpsI GAGTC 6 cut(s) 23, 334, 1348, 1726, 3118, 3605
Ppu21I YACGTR 1 cut(s) 928
PpuMI RGGWCCY 1 cut(s) 1646
PscI ACATGT 1 cut(s) 268
PshBI ATTAAT 1 cut(s) 3428
Psp124BI GAGCTC 4 cut(s) 203, 2684, 3343, 4363
Psp5II RGGWCCY 1 cut(s) 1646
Psp6I CCWGG 4 cut(s) 123, 415, 1049, 3464
PspFI CCCAGC 1 cut(s) 3592
PspGI CCWGG 4 cut(s) 123, 415, 1049, 3464
PspN4I GGNNCC 9 cut(s) 6, 1518, 1753, 1908, 2228, 2282, 3463, 3763, 4287
PspOMI GGGCCC 1 cut(s) 2280
PspPPI RGGWCCY 1 cut(s) 1646
PspXI VCTCGAGB 1 cut(s) 1675
PsrI GAACNNNNNNTAC 2 cut(s) 4158, 4190
PstI CTGCAG 4 cut(s) 1012, 1309, 3627, 3880
PstNI CAGNNNCTG 4 cut(s) 230, 839, 1670, 3884
PsuI RGATCY 6 cut(s) 4, 673, 866, 1516, 2992, 3761
PsyI GACNNNGTC 1 cut(s) 3884
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 8 cut(s) 312, 880, 2057, 2248, 2493, 2751, 3740, 3746
RsaNI GTAC 8 cut(s) 311, 879, 2056, 2247, 2492, 2750, 3739, 3745
RseI CAYNNNNRTG 5 cut(s) 510, 641, 2303, 3173, 3773
SacI GAGCTC 4 cut(s) 203, 2684, 3343, 4363
SacII CCGCGG 1 cut(s) 2581
ScaI AGTACT 1 cut(s) 880
SchI GAGTC 6 cut(s) 23, 334, 1349, 1726, 3119, 3605
ScrFI CCNGG 5 cut(s) 125, 417, 1051, 2132, 3466
SfcI CTRYAG 6 cut(s) 166, 1008, 1305, 3623, 3730, 3876
Sfr274I CTCGAG 3 cut(s) 56, 1675, 3713
Sfr303I CCGCGG 1 cut(s) 2581
SfuI TTCGAA 2 cut(s) 1424, 2664
SgrBI CCGCGG 1 cut(s) 2581
SinI GGWCC 5 cut(s) 330, 919, 1646, 1907, 3461
SlaI CTCGAG 3 cut(s) 56, 1675, 3713
SmiMI CAYNNNNRTG 5 cut(s) 510, 641, 2303, 3173, 3773
SphI GCATGC 1 cut(s) 2414
SseBI AGGCCT 1 cut(s) 1779
SspI AATATT 4 cut(s) 1060, 2620, 2922, 3558
SspMI CTAG 8 cut(s) 777, 1218, 1790, 2091, 2156, 2741, 3260, 4356
SstI GAGCTC 4 cut(s) 203, 2684, 3343, 4363
StuI AGGCCT 1 cut(s) 1779
StyD4I CCNGG 5 cut(s) 123, 415, 1049, 2130, 3464
StyI CCWWGG 5 cut(s) 333, 430, 3114, 3410, 3918
TaaI ACNGT 9 cut(s) 1397, 1726, 2222, 2427, 2491, 3886, 3976, 4003, 4045
TaiI ACGT 4 cut(s) 930, 1324, 3449, 4213
TaqII GACCGA 2 cut(s) 1433, 4281
TatI WGTACW 5 cut(s) 310, 878, 2491, 2749, 3738
TauI GCSGC 3 cut(s) 1496, 3076, 3652
TscAI CASTG 5 cut(s) 1612, 1790, 2116, 2501, 3889
TseFI GTSAC 7 cut(s) 478, 792, 1397, 2107, 2701, 3131, 3166
Tsp45I GTSAC 7 cut(s) 478, 792, 1397, 2107, 2701, 3131, 3166
TspGWI ACGGA 5 cut(s) 901, 2388, 2495, 3623, 4293
TspRI CASTG 5 cut(s) 1612, 1790, 2116, 2501, 3889
Tth111I GACNNNGTC 1 cut(s) 3884
Vha464I CTTAAG 2 cut(s) 2912, 3416
VpaK11BI GGWCC 5 cut(s) 330, 919, 1646, 1907, 3461
VspI ATTAAT 1 cut(s) 3428
XceI RCATGY 5 cut(s) 272, 311, 1240, 2414, 4076
XhoI CTCGAG 3 cut(s) 56, 1675, 3713
XmiI GTMKAC 1 cut(s) 976
XmnI GAANNNNTTC 4 cut(s) 47, 768, 2426, 2871
XspI CTAG 8 cut(s) 777, 1218, 1790, 2091, 2156, 2741, 3260, 4356
ZrmI AGTACT 1 cut(s) 880
Zsp2I ATGCAT 1 cut(s) 1600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.