Rw3G023240

DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005736
Physical Location & Seq
Forward (+)
29086508 .. 29097561
11054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G023240.1

Sequence Viewer

Length: 4164 bp
ATGGGGATTCCGGTTTCAAAGGGTAATAAGGGTTCGAAATCGAGGAAGCTGGCGAATGAGAACCGAGGAGGGTTTTATGAAATGGAAACCACGTCTGAGTGGCTCAATGATACTATTCCTCAGAAACCAGGGAACTACCATGATGCTGATTTTGCGCCACAAAGTGCCACTGATAGAGTAATATATGAACCTCCTTATCTGGATATTGGTGATGGTTTCACATACAAGATTCCACCAATTTATGATAAACTGGTGACATCTTTACACTTGCCGAGCTTTTCTGATTTTCGGGTGGAGGAAGTTTACTTAAAAGGTACATTGGATTTGGGATCCTTAGCAGAAATGATGGGTAGCGATAAAAGGTTTGGTCCAAAAAATCGGGCAGGAATGGGGGAGCCCTATCCCCAGTATGAGTCCCTTCAGGCAAGATTAAAGGCCTTGTCCACTTCTACCTCTGCTCAAAAGTTCAGCCTCAAAGTTTCTGATATTGGGTTGAATTCCTCCATCCCAGAGGGGGCAGCTGGAACTATAAAGAGGTCTATTTTGTCCGAGGGTGGTGTCTTGCAAGTCTACTATGTGAAGGTTTTGGAGAAGGGAGACACATATGAGATAATTGAACGAAGCCTACCCAAGAAGCAAAAGGTACAGAAAGATCCTTCTCAGATTGAGAAGGAGGAAATGGAAAGGATGGGAAGAGTTTGGGTAAACATTGTAAGAAGAGACATACCAAAGCATCATAGAATGTTTACTACTTTTCACCGCAAGCAACTAATTGATGCCAAGAGGGTCTCAGAGAACTGTCAAAGAGAGGTAAAAATGAAGGTGAGTAGATCACTTAAAGTGATGAGGGGTGCTGCAATTCGCACAAGGAAACTAGCCAGAGACATGCTACTGCTTTGGAAGAGAATAGATAAAGAGATGGCAGAAGTGAGGAAGAAAGAGGAAAAAGAAGCTATTGAAATGCGCAAACGTCTGGAGGAGGAAAGAGAAGCAAAGAGACAGGAGCAAAGACTCAATTTTCTCATAAAACAAACCGAACTTTACAGTCACTTCATGCAGAACAAGTTGAGTTCACAGCCATCTGAAGACTTGCCTGTAGGAGATGAAAACCAAGATGTATCTCCAAGCTCCTCAGATATTGAGAATGTTGAAGAAGATTCTGAGGAAGCTGAACTGAAGAAGGAGGCATTAAAAGCTGCTCAAGATGCAGTTTCAAAGCAGAAAAAGTTAACTAGTGCATTTGATGACGAGTGTCTGAGGCTGCGTGAAGCCTCTGAACCTGAGGCACCACAGGATGTTGCTGGAGCTAATAACATTGATCTGCATAACCCTTCCACTATGCCTGTTACATCAACCGTTCAGACACCAGAGTTGTTCAAAGGCTCCCTTAAAGAATACCAGCTAAAAGGCCTCCAGTGGCTGGTTAATTGTTATGAGCAGGGTTTAAATGGCATCCTTGCTGATGAGATGGGCCTGGGTAAGACCATCCAGGCCATGGCATTTTTGGCTCATTTGGCTGAGGAGAAAAATATATGGGGGCCTTTTCTGGTTGTTGCTCCTGCTTCCGTCTTGAACAACTGGGCTGATGAAATTAGTCGTTTCTGCCCCGACTTAAAAACTCTTCCGTATTGGGGTGGCCTTCAAGAGCGTATGGTACTTAGGAAGAAGATCAACGCTAAGAAACTTTACCGTAGGGATGCTGGGTTTCACATTCTCATTACCAGTTACCAGTTGCTAGTGGGTGATGAGAAGTGTTTCCGGCGTGTGAAATGGCAATATATGGTATTGGATGAAGCACAAGCTATCAAAAGTTCTAACAGTATAAGATGGAAGACACTGCTCAGCTTTAATTGTCGAAATCGTTTGCTGCTTACTGGTACACCTATCCAGAATAATATGGCAGAGTTGTGGGCCCTACTACATTTCATTATGCCAACCTTATTTGACAGTCATGAACAATTCAATGAGTGGTTCTCTAAAGGAATTGAAAATCATGCGGAACATGGGGGTACTTTGAACGAGCACCAGCTTAACAGATTGCATTCGATATTGAAGCCATTCATGCTCCGTCGAGTTAAGACAGATGTGATTTCTGAGCTGACCAGGAAAACTGAAGTTACTGTGCACTGCAAGTTGAGTTCTCGGCAACAAGCTTTTTATCAAGCTATCAAAAACAAGATATCTCTTGCTGAGCTGTTTGACAACAATCGTGGGCATCTTAATGAGAAGAAAATTTTGAACTTAATGAATATTGTCATTCAGCTAAGAAAGGTCTGCAACCATCCTGAGTTATTTGAGAGGAATGAAGGAAGCACATATCTTCACTTTGGTGAGATTTCAAATTCTCTTCTTCCTCCACCATTTGGGGAGTTGGAGGACGTACACTACTCGGGAGGTCAAAATCCTATAACATACGAGGTCCCCAAACTTTTATATCAAGAAATACTCCAGAATTCTGAAACGTTTTGCTCTGCAGTTCGGCATGGTGTCTACAGTGAATCTTTTCAGAAACATTTTAACATATTTTCACCGCAAAATGTGTATCGATCAATATTCTTGCAAGAGAATGACTCAGATGAGTTGTCGGTTGGAAGTGGAACGTTTGGTTTTACTCATCTGATGGATCTGTCCCCTGCAGAGGTTGCATTTTTAGGAACTGGTTCTTTTATGGAGCGGCTAATGTTTTCCATTATGAGATGGGACCGGCAGTTTTTGGATGGATTGATGGACACACTAATGGAAACCATGGATGATGATGATTCTGAATGCAGCTTCCTTGATAGTGGAAAAGTGAGAGCTGTTACACGTATGTTGCTGATGCCTTCAAGATCTATAACTACCATCTTTCAGAAGAAATTGGCCACTGGACCTGGTGGTACTCCATTTGAGGGTCTAGTTGTCTCTCATCGGGACAGGCTTCTCTCTAATATTAGGCTTCTCCGTTCGACATACACATTCATCCCCCGAACTAGAGCTCCCCCAGTTAATGTCCATTGCTCAGATCGAAACTTCACTTACAAAATGAGTGAAGAACAACAGTACCCCTGGGTGAAGAGGTTGTTTTCTGGTTTTGCGCGAACATCTGACTACAATGGACCCAGAAAACCTGAGACTCCTCATCATCTAATACAAGAGATTGATTCTGAACTGCCTGTTTCACATTCAGCTCTTCAGTTGACATACAGAATTTTTGGGTCTTGTCCTCCAATGCAAAGCTTCGACCCGTCAAAAATGCTCACTGATTCGGGGAAACTTCAGACACTTGATATATTGTTGAAACGCTTGAGGGCAGAAAATCATCGTGTTCTCTTGTTTGCACAGATGACAAAGATGCTGAATATTCTTGAGGACTACATGAACTACAGGAAATATAGATACCTCAGACTGGATGGATCTTCCACCATTATGGATCGTAGAGACATGGTCAGAGACTTCCAGCAACGAAGTGATATTTTTGTTTTCTTGCTGAGTACAAGAGCTGGTGGACTAGGCATTAACTTGACAGCTGCAGATACTGTCATATTTTATGAAAGTGATTGGAATCCGACCCTGGATCTACAGGCAATGGATAGAGCTCATCGGTTGGGCCAGACTAAAGATGTTACCGTCTACCGCCTAATTTGTAAAGAAACGGTTGAGGAGAAGATTCTGCAAAGAGCAAGTCAGAAAAATACTGTGCAGCAGCTTGTAATGATGGGTGGTCATGTTCAAGGTGACCTTTTGGCTCCCGAGGATGTTGTATCTCTACTTCTCGATGATGCCCAGTTGGAGCAGAAATTAAGAGAAGCTCCATCACAGGTTAAGGATAGACAAAAGAAGAAACAGACAAAGGGTATACGGGTAGATGCAGAAGGTGATGCCTCTTTGGAAGATTTAACAAACACTGCTGCATCACAAGGTACTGGAAATGAGGAATCTCCGGATGTCGAAAGATCAAAATCCAATAATAAAAAGAGGAAAACGGTGCCTGACAAGCATACTCCTCGGCCGAAGAATCCTCAAAGTATGGATGAACCAGAGGGCTATGAATTGGAGGATTCCTTACAAAATACTGATCCACAAGGTACAAGACCCAAGAGGCCAAAGAGGTCAAAGAAGAGTGTAAATGAAACTCTTGAACCTGCATTTACAGCGGCATCCCCTGTGGTCCCGAGGCAGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBINO Helicase_C SNF2_N

Protein Analysis

1387

Amino Acids

158.62

Weight (kDa)

8.33

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBINO PF13892 228 - 358 1.2e-44 DNA-binding domain
ResIII PF04851 462 - 629 9.8e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 466 - 764 3.6e-85 SNF2-related domain
Helicase_C PF00271 1086 - 1196 2.4e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 965
Acc36I ACCTGC 1 cut(s) 4129
AccB1I GGYRCC 2 cut(s) 1285, 3964
AccB7I CCANNNNNTGG 3 cut(s) 1420, 1495, 2363
AccBSI CCGCTC 1 cut(s) 2674
AccI GTMKAC 4 cut(s) 570, 2490, 3609, 3835
AccII CGCG 1 cut(s) 3076
AccIII TCCGGA 1 cut(s) 3919
AciI CCGC 6 cut(s) 760, 1997, 2531, 2674, 3613, 4133
AclI AACGTT 2 cut(s) 2462, 2600
AclWI GGATC 8 cut(s) 324, 337, 647, 2631, 3400, 3417, 3561, 4049
AcoI YGGCCR 2 cut(s) 2859, 3986
AcsI RAATTY 5 cut(s) 496, 2232, 2341, 2452, 3186
AcuI CTGAAG 6 cut(s) 404, 1104, 1196, 2133, 3155, 3239
AdeI CACNNNGTG 1 cut(s) 164
AfiI CCNNNNNNNGG 8 cut(s) 514, 1420, 1495, 1631, 2363, 2638, 2888, 3385
AflIII ACRYGT 1 cut(s) 2804
AhlI ACTAGT 1 cut(s) 1232
AjiI CACGTC 1 cut(s) 93
AjnI CCWGG 7 cut(s) 127, 1473, 1488, 2102, 2869, 3044, 3549
AleI CACNNNNGTG 1 cut(s) 2328
AloI GAACNNNNNNTCC 2 cut(s) 2959, 2991
Alw21I GWGCWC 4 cut(s) 2025, 2127, 2977, 3577
Alw26I GTCTC 9 cut(s) 591, 714, 793, 876, 991, 2905, 3104, 3411, 3423
Alw44I GTGCAC 1 cut(s) 2123
AlwI GGATC 8 cut(s) 324, 337, 647, 2631, 3400, 3417, 3561, 4049
AlwNI CAGNNNCTG 2 cut(s) 1420, 3515
Ama87I CYCGRG 3 cut(s) 2389, 3728, 4150
Aor13HI TCCGGA 1 cut(s) 3919
ApaI GGGCCC 1 cut(s) 1915
ApaLI GTGCAC 1 cut(s) 2123
ApoI RAATTY 5 cut(s) 496, 2232, 2341, 2452, 3186
ArsI GACNNNNNNTTYG 2 cut(s) 3646, 3678
Asp700I GAANNNNTTC 4 cut(s) 1754, 2057, 2502, 2659
AspLEI GCGC 3 cut(s) 157, 966, 3076
AsuII TTCGAA 1 cut(s) 35
AvaI CYCGRG 3 cut(s) 2389, 3728, 4150
AvaII GGWCC 6 cut(s) 368, 2419, 2701, 2867, 3095, 4147
AxyI CCTNAGG 1 cut(s) 1281
BaeGI GKGCMC 2 cut(s) 1915, 2127
BaeI ACNNNNGTAYC 2 cut(s) 3023, 3056
BalI TGGCCA 1 cut(s) 2861
BamHI GGATCC 1 cut(s) 329
BanI GGYRCC 2 cut(s) 1285, 3964
BanII GRGCYC 4 cut(s) 399, 1915, 2977, 3577
BarI GAAGNNNNNNTAC 2 cut(s) 3732, 3764
BbsI GAAGAC 2 cut(s) 1092, 1838
Bbv12I GWGCWC 4 cut(s) 2025, 2127, 2977, 3577
BbvCI CCTCAGC 1 cut(s) 1518
BcgI CGANNNNNNTGC 2 cut(s) 3965, 3999
BciT130I CCWGG 7 cut(s) 129, 1475, 1490, 2104, 2871, 3046, 3551
BcoDI GTCTC 9 cut(s) 591, 714, 793, 876, 991, 2905, 3104, 3411, 3423
BcuI ACTAGT 1 cut(s) 1232
BfaI CTAG 6 cut(s) 875, 1233, 1736, 2894, 2970, 3488
BfmI CTRYAG 7 cut(s) 1095, 2472, 2491, 2634, 3361, 3507, 3557
BfuAI ACCTGC 1 cut(s) 4129
BglII AGATCT 1 cut(s) 2828
BlpI GCTNAGC 2 cut(s) 1841, 2190
Bme1390I CCNGG 7 cut(s) 129, 1475, 1490, 2104, 2871, 3046, 3551
Bme18I GGWCC 6 cut(s) 368, 2419, 2701, 2867, 3095, 4147
BmeT110I CYCGRG 3 cut(s) 2389, 3728, 4150
BmgBI CACGTC 1 cut(s) 93
BmrFI CCNGG 7 cut(s) 129, 1475, 1490, 2104, 2871, 3046, 3551
BmrI ACTGGG 4 cut(s) 400, 1588, 2975, 3757
BmuI ACTGGG 4 cut(s) 400, 1588, 2975, 3757
BoxI GACNNNNGTC 1 cut(s) 1251
BpiI GAAGAC 2 cut(s) 1092, 1838
BplI GAGNNNNNCTC 4 cut(s) 1958, 1990, 2555, 2587
BpmI CTGGAG 4 cut(s) 995, 1323, 1397, 2432
Bpu10I CCTNAGC 2 cut(s) 334, 1518
Bpu1102I GCTNAGC 2 cut(s) 1841, 2190
Bpu14I TTCGAA 1 cut(s) 35
BpuEI CTTGAG 3 cut(s) 1185, 3304, 3365
Bsa29I ATCGAT 1 cut(s) 2545
BsaAI YACGTR 1 cut(s) 2807
BsaBI GATNNNNATC 2 cut(s) 3540, 3937
BsaI GGTCTC 1 cut(s) 793
BsaWI WCCGGW 2 cut(s) 10, 3919
BsaXI ACNNNNNCTCC 2 cut(s) 2959, 2989
Bsc4I CCNNNNNNNGG 8 cut(s) 514, 1420, 1495, 1631, 2363, 2638, 2888, 3385
Bse118I RCCGGY 1 cut(s) 2703
Bse21I CCTNAGG 1 cut(s) 1281
Bse3DI GCAATG 2 cut(s) 2992, 3570
Bse8I GATNNNNATC 2 cut(s) 3540, 3937
BseAI TCCGGA 1 cut(s) 3919
BseBI CCWGG 7 cut(s) 129, 1475, 1490, 2104, 2871, 3046, 3551
BseCI ATCGAT 1 cut(s) 2545
BseJI GATNNNNATC 2 cut(s) 3540, 3937
BseLI CCNNNNNNNGG 8 cut(s) 514, 1420, 1495, 1631, 2363, 2638, 2888, 3385
BseMI GCAATG 2 cut(s) 2992, 3570
BseRI GAGGAG 7 cut(s) 81, 992, 1120, 1535, 3105, 3653, 3972
BseSI GKGCMC 2 cut(s) 1915, 2127
BseX3I CGGCCG 1 cut(s) 3986
BseYI CCCAGC 1 cut(s) 1700
BsgI GTGCAG 1 cut(s) 3698
Bsh1236I CGCG 1 cut(s) 3076
Bsh1285I CGRYCG 1 cut(s) 3989
BshNI GGYRCC 2 cut(s) 1285, 3964
BshVI ATCGAT 1 cut(s) 2545
BsiEI CGRYCG 1 cut(s) 3989
BsiHKAI GWGCWC 4 cut(s) 2025, 2127, 2977, 3577
BsiHKCI CYCGRG 3 cut(s) 2389, 3728, 4150
BsiSI CCGG 4 cut(s) 11, 1759, 2704, 3920
BslFI GGGAC 6 cut(s) 400, 2405, 2614, 2714, 2924, 4133
BslI CCNNNNNNNGG 8 cut(s) 514, 1420, 1495, 1631, 2363, 2638, 2888, 3385
BsmAI GTCTC 9 cut(s) 591, 714, 793, 876, 991, 2905, 3104, 3411, 3423
BsmFI GGGAC 6 cut(s) 400, 2405, 2614, 2714, 2924, 4133
BsmI GAATGC 2 cut(s) 2041, 2771
Bso31I GGTCTC 1 cut(s) 793
BsoBI CYCGRG 3 cut(s) 2389, 3728, 4150
Bsp119I TTCGAA 1 cut(s) 35
Bsp120I GGGCCC 1 cut(s) 1911
Bsp1286I GDGCHC 6 cut(s) 399, 1915, 2025, 2127, 2977, 3577
Bsp13I TCCGGA 1 cut(s) 3919
Bsp1720I GCTNAGC 2 cut(s) 1841, 2190
Bsp19I CCATGG 2 cut(s) 1494, 2745
BspACI CCGC 6 cut(s) 760, 1997, 2531, 2674, 3613, 4133
BspDI ATCGAT 1 cut(s) 2545
BspEI TCCGGA 1 cut(s) 3919
BspFNI CGCG 1 cut(s) 3076
BspHI TCATGA 1 cut(s) 1951
BspMAI CTGCAG 3 cut(s) 2476, 2638, 3511
BspMI ACCTGC 1 cut(s) 4129
BspPI GGATC 8 cut(s) 324, 337, 647, 2631, 3400, 3417, 3561, 4049
BspQI GCTCTTC 1 cut(s) 3174
BspT104I TTCGAA 1 cut(s) 35
BspT107I GGYRCC 2 cut(s) 1285, 3964
BspTNI GGTCTC 1 cut(s) 793
BsrBI CCGCTC 1 cut(s) 2674
BsrDI GCAATG 2 cut(s) 2992, 3570
BsrFI RCCGGY 1 cut(s) 2703
BssAI RCCGGY 1 cut(s) 2703
BssNAI GTATAC 1 cut(s) 3836
BssT1I CCWWGG 2 cut(s) 1494, 2745
Bst1107I GTATAC 1 cut(s) 3836
Bst2UI CCWGG 7 cut(s) 129, 1475, 1490, 2104, 2871, 3046, 3551
Bst6I CTCTTC 8 cut(s) 688, 712, 896, 1626, 2352, 3047, 3174, 4091
BstAPI GCANNNNNTGC 1 cut(s) 2642
BstBAI YACGTR 1 cut(s) 2807
BstBI TTCGAA 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 51, 764
BstDSI CCRYGG 2 cut(s) 1494, 2745
BstEII GGTNACC 1 cut(s) 3713
BstFNI CGCG 1 cut(s) 3076
BstHHI GCGC 3 cut(s) 157, 966, 3076
BstMAI GTCTC 9 cut(s) 591, 714, 793, 876, 991, 2905, 3104, 3411, 3423
BstMCI CGRYCG 1 cut(s) 3989
BstMWI GCNNNNNNNGC 9 cut(s) 152, 1193, 1205, 1505, 1514, 2062, 2642, 3973, 4130
BstNI CCWGG 7 cut(s) 129, 1475, 1490, 2104, 2871, 3046, 3551
BstNSI RCATGY 1 cut(s) 889
BstPAI GACNNNNGTC 1 cut(s) 1251
BstPI GGTNACC 1 cut(s) 3713
BstSCI CCNGG 7 cut(s) 127, 1473, 1488, 2102, 2869, 3044, 3549
BstSFI CTRYAG 7 cut(s) 1095, 2472, 2491, 2634, 3361, 3507, 3557
BstSLI GKGCMC 2 cut(s) 1915, 2127
BstUI CGCG 1 cut(s) 3076
BstV2I GAAGAC 2 cut(s) 1092, 1838
BstX2I RGATCY 6 cut(s) 329, 652, 2623, 2828, 3392, 3553
BstXI CCANNNNNNTGG 1 cut(s) 3406
BstYI RGATCY 6 cut(s) 329, 652, 2623, 2828, 3392, 3553
BstZ17I GTATAC 1 cut(s) 3836
BstZI CGGCCG 1 cut(s) 3986
Bsu15I ATCGAT 1 cut(s) 2545
Bsu36I CCTNAGG 1 cut(s) 1281
BsuTUI ATCGAT 1 cut(s) 2545
BtgI CCRYGG 2 cut(s) 1494, 2745
BtrI CACGTC 1 cut(s) 93
BtsI GCAGTG 3 cut(s) 1835, 2125, 3882
BtsIMutI CAGTG 8 cut(s) 168, 1421, 1835, 2125, 2500, 2862, 3237, 3882
BveI ACCTGC 1 cut(s) 4129
Cac8I GCNNGC 2 cut(s) 51, 764
CaiI CAGNNNCTG 2 cut(s) 1420, 3515
CciI TCATGA 1 cut(s) 1951
CfoI GCGC 3 cut(s) 157, 966, 3076
Cfr10I RCCGGY 1 cut(s) 2703
ClaI ATCGAT 1 cut(s) 2545
CsiI ACCWGGT 1 cut(s) 2869
CspCI CAANNNNNGTGG 2 cut(s) 2191, 2226
DraI TTTAAA 1 cut(s) 1446
DraIII CACNNNGTG 1 cut(s) 164
EaeI YGGCCR 2 cut(s) 2859, 3986
EagI CGGCCG 1 cut(s) 3986
Eam1104I CTCTTC 8 cut(s) 688, 712, 896, 1626, 2352, 3047, 3174, 4091
EarI CTCTTC 8 cut(s) 688, 712, 896, 1626, 2352, 3047, 3174, 4091
Ecl136II GAGCTC 2 cut(s) 2975, 3575
EclXI CGGCCG 1 cut(s) 3986
Eco130I CCWWGG 2 cut(s) 1494, 2745
Eco147I AGGCCT 2 cut(s) 437, 1410
Eco24I GRGCYC 4 cut(s) 399, 1915, 2977, 3577
Eco31I GGTCTC 1 cut(s) 793
Eco32I GATATC 1 cut(s) 2181
Eco47I GGWCC 6 cut(s) 368, 2419, 2701, 2867, 3095, 4147
Eco52I CGGCCG 1 cut(s) 3986
Eco53kI GAGCTC 2 cut(s) 2975, 3575
Eco57I CTGAAG 6 cut(s) 404, 1104, 1196, 2133, 3155, 3239
Eco81I CCTNAGG 1 cut(s) 1281
Eco88I CYCGRG 3 cut(s) 2389, 3728, 4150
Eco91I GGTNACC 1 cut(s) 3713
EcoICRI GAGCTC 2 cut(s) 2975, 3575
EcoO109I RGGNCCY 3 cut(s) 1538, 1912, 2419
EcoO65I GGTNACC 1 cut(s) 3713
EcoRI GAATTC 2 cut(s) 496, 2452
EcoRII CCWGG 7 cut(s) 127, 1473, 1488, 2102, 2869, 3044, 3549
EcoRV GATATC 1 cut(s) 2181
EcoT14I CCWWGG 2 cut(s) 1494, 2745
EcoT38I GRGCYC 4 cut(s) 399, 1915, 2977, 3577
ErhI CCWWGG 2 cut(s) 1494, 2745
FalI AAGNNNNNCTT 4 cut(s) 1371, 1403, 3702, 3734
FaqI GGGAC 6 cut(s) 400, 2405, 2614, 2714, 2924, 4133
FauNDI CATATG 1 cut(s) 604
FblI GTMKAC 4 cut(s) 570, 2490, 3609, 3835
FriOI GRGCYC 4 cut(s) 399, 1915, 2977, 3577
FspBI CTAG 6 cut(s) 875, 1233, 1736, 2894, 2970, 3488
FspI TGCGCA 1 cut(s) 965
GlaI GCGC 3 cut(s) 156, 965, 3075
GsaI CCCAGC 1 cut(s) 1704
GsuI CTGGAG 4 cut(s) 995, 1323, 1397, 2432
HapII CCGG 4 cut(s) 11, 1759, 2704, 3920
HhaI GCGC 3 cut(s) 157, 966, 3076
Hin6I GCGC 3 cut(s) 155, 964, 3074
HinP1I GCGC 3 cut(s) 155, 964, 3074
HincII GTYRAC 2 cut(s) 1230, 3177
HindII GTYRAC 2 cut(s) 1230, 3177
HindIII AAGCTT 2 cut(s) 2151, 3214
HpaI GTTAAC 1 cut(s) 1230
HpaII CCGG 4 cut(s) 11, 1759, 2704, 3920
Hpy99I CGWCG 1 cut(s) 2073
HpyCH4IV ACGT 6 cut(s) 92, 970, 2379, 2462, 2600, 2806
HpyF10VI GCNNNNNNNGC 9 cut(s) 152, 1193, 1205, 1505, 1514, 2062, 2642, 3973, 4130
HpySE526I ACGT 6 cut(s) 92, 970, 2379, 2462, 2600, 2806
HspAI GCGC 3 cut(s) 155, 964, 3074
Kpn2I TCCGGA 1 cut(s) 3919
KspAI GTTAAC 1 cut(s) 1230
LguI GCTCTTC 1 cut(s) 3174
MabI ACCWGGT 1 cut(s) 2869
MaeI CTAG 6 cut(s) 875, 1233, 1736, 2894, 2970, 3488
MaeII ACGT 6 cut(s) 92, 970, 2379, 2462, 2600, 2806
MaeIII GTNAC 8 cut(s) 253, 1046, 1345, 1724, 2116, 2800, 3601, 3713
MbiI CCGCTC 1 cut(s) 2674
MflI RGATCY 6 cut(s) 329, 652, 2623, 2828, 3392, 3553
MhlI GDGCHC 6 cut(s) 399, 1915, 2025, 2127, 2977, 3577
MlsI TGGCCA 1 cut(s) 2861
MluNI TGGCCA 1 cut(s) 2861
MlyI GAGTC 4 cut(s) 422, 1005, 2564, 3106
MmeI TCCRAC 4 cut(s) 2352, 2569, 3569, 3747
Mox20I TGGCCA 1 cut(s) 2861
MroI TCCGGA 1 cut(s) 3919
MroXI GAANNNNTTC 4 cut(s) 1754, 2057, 2502, 2659
MscI TGGCCA 1 cut(s) 2861
MslI CAYNNNNRTG 4 cut(s) 2220, 2328, 2735, 3404
Msp20I TGGCCA 1 cut(s) 2861
MspA1I CMGCKG 3 cut(s) 521, 3506, 4133
MspI CCGG 4 cut(s) 11, 1759, 2704, 3920
MspR9I CCNGG 7 cut(s) 129, 1475, 1490, 2104, 2871, 3046, 3551
Mva1269I GAATGC 2 cut(s) 2041, 2771
MvaI CCWGG 7 cut(s) 129, 1475, 1490, 2104, 2871, 3046, 3551
MvnI CGCG 1 cut(s) 3076
MwoI GCNNNNNNNGC 9 cut(s) 152, 1193, 1205, 1505, 1514, 2062, 2642, 3973, 4130
NcoI CCATGG 2 cut(s) 1494, 2745
NdeI CATATG 1 cut(s) 604
NmeAIII GCCGAG 3 cut(s) 297, 2122, 3964
NmuCI GTSAC 3 cut(s) 253, 1046, 3713
NsbI TGCGCA 1 cut(s) 965
NspI RCATGY 1 cut(s) 889
NspV TTCGAA 1 cut(s) 35
OliI CACNNNNGTG 1 cut(s) 2328
PagI TCATGA 1 cut(s) 1951
PasI CCCWGGG 1 cut(s) 3045
PceI AGGCCT 2 cut(s) 437, 1410
PciSI GCTCTTC 1 cut(s) 3174
PctI GAATGC 2 cut(s) 2041, 2771
PdmI GAANNNNTTC 4 cut(s) 1754, 2057, 2502, 2659
PflFI GACNNNGTC 1 cut(s) 3422
PflMI CCANNNNNTGG 3 cut(s) 1420, 1495, 2363
PleI GAGTC 4 cut(s) 421, 1005, 2564, 3106
PpsI GAGTC 4 cut(s) 421, 1005, 2564, 3106
Ppu21I YACGTR 1 cut(s) 2807
PpuMI RGGWCCY 1 cut(s) 2419
PshAI GACNNNNGTC 1 cut(s) 1251
Psp124BI GAGCTC 2 cut(s) 2977, 3577
Psp1406I AACGTT 2 cut(s) 2462, 2600
Psp5II RGGWCCY 1 cut(s) 2419
Psp6I CCWGG 7 cut(s) 127, 1473, 1488, 2102, 2869, 3044, 3549
PspEI GGTNACC 1 cut(s) 3713
PspFI CCCAGC 1 cut(s) 1700
PspGI CCWGG 7 cut(s) 127, 1473, 1488, 2102, 2869, 3044, 3549
PspOMI GGGCCC 1 cut(s) 1911
PspPPI RGGWCCY 1 cut(s) 2419
PsrI GAACNNNNNNTAC 4 cut(s) 16, 48, 609, 641
PstI CTGCAG 3 cut(s) 2476, 2638, 3511
PstNI CAGNNNCTG 2 cut(s) 1420, 3515
PsuI RGATCY 6 cut(s) 329, 652, 2623, 2828, 3392, 3553
PsyI GACNNNGTC 1 cut(s) 3422
PvuII CAGCTG 2 cut(s) 521, 3506
RseI CAYNNNNRTG 4 cut(s) 2220, 2328, 2735, 3404
SacI GAGCTC 2 cut(s) 2977, 3577
SapI GCTCTTC 1 cut(s) 3174
SchI GAGTC 4 cut(s) 422, 1005, 2564, 3106
ScrFI CCNGG 7 cut(s) 129, 1475, 1490, 2104, 2871, 3046, 3551
SduI GDGCHC 6 cut(s) 399, 1915, 2025, 2127, 2977, 3577
SexAI ACCWGGT 1 cut(s) 2869
SfcI CTRYAG 7 cut(s) 1095, 2472, 2491, 2634, 3361, 3507, 3557
SfuI TTCGAA 1 cut(s) 35
SinI GGWCC 6 cut(s) 368, 2419, 2701, 2867, 3095, 4147
SmiMI CAYNNNNRTG 4 cut(s) 2220, 2328, 2735, 3404
SmlI CTYRAG 3 cut(s) 1200, 3283, 3344
SmoI CTYRAG 3 cut(s) 1200, 3283, 3344
SpeI ACTAGT 1 cut(s) 1232
SseBI AGGCCT 2 cut(s) 437, 1410
SsiI CCGC 6 cut(s) 760, 1997, 2531, 2674, 3613, 4133
SspI AATATT 4 cut(s) 2251, 2553, 2929, 3340
SspMI CTAG 6 cut(s) 875, 1233, 1736, 2894, 2970, 3488
SstI GAGCTC 2 cut(s) 2977, 3577
StuI AGGCCT 2 cut(s) 437, 1410
StyD4I CCNGG 7 cut(s) 127, 1473, 1488, 2102, 2869, 3044, 3549
StyI CCWWGG 2 cut(s) 1494, 2745
TaiI ACGT 6 cut(s) 95, 973, 2382, 2465, 2603, 2809
TatI WGTACW 1 cut(s) 3470
TauI GCSGC 2 cut(s) 2677, 4136
TscAI CASTG 8 cut(s) 175, 1421, 1842, 2132, 2500, 2869, 3244, 3889
TseFI GTSAC 3 cut(s) 253, 1046, 3713
Tsp45I GTSAC 3 cut(s) 253, 1046, 3713
TspGWI ACGGA 4 cut(s) 1555, 1614, 2057, 2930
TspRI CASTG 8 cut(s) 175, 1421, 1842, 2132, 2500, 2869, 3244, 3889
Tth111I GACNNNGTC 1 cut(s) 3422
Van91I CCANNNNNTGG 3 cut(s) 1420, 1495, 2363
VneI GTGCAC 1 cut(s) 2123
VpaK11BI GGWCC 6 cut(s) 368, 2419, 2701, 2867, 3095, 4147
XapI RAATTY 5 cut(s) 496, 2232, 2341, 2452, 3186
XceI RCATGY 1 cut(s) 889
XcmI CCANNNNNNNNNTGG 2 cut(s) 1492, 1501
XmiI GTMKAC 4 cut(s) 570, 2490, 3609, 3835
XmnI GAANNNNTTC 4 cut(s) 1754, 2057, 2502, 2659
XspI CTAG 6 cut(s) 875, 1233, 1736, 2894, 2970, 3488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.