Rh3AG257100

DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0005736
Physical Location & Seq
Forward (+)
28398832 .. 28409656
10825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG257100.1

Sequence Viewer

Length: 4506 bp
ATGGACCACAGGCGCCAGTCCAAGGATTCTCTCTCCTCCTCCTACTCCAACCTCTTCAATCTTGAGCTTCCACAGCCAGATGATGATTTTGATTATTACGGCAATAGCAGTCAGGATGAGAGCAGAGGTAGCCAAGGTGGAGCTGCTGGTAATGGGATCATGTCCGACCGCGAACTGAATTCAGTAAAGAAAAGGAGGCGGTCTCAAAACAGTGATTACGAGGAGGATGACAGTTATTACGGGACGCACATTACAGAGGACAAGTATAGATCAATGCTTGGAGAACACATTCAGAAGTACAAAAGGAGGTTCAAGGATTCCCCGTCAAGTCCTGCACCAATCCAGATGGGGATTCCGGTTTCAAAGGGTAATAAGGGTTCGAAATCGAGGAAGCTGGCGAATGAGAACCGAGGAGGGTTCTATGAATTGGAAACCACGTCTGAGTGGCTCAATGATACCATTCCTCAGAAACCAGGGAACTACCATGATGCTGATTTTGCGCCACAAAGTGCCGCTGATAGAGTAATATATGAACCTCCTTATCTGGATATTGGAGATGGTTTCACATACAAGATTCCACCAATTTATGATAAACTGGTGACATCTTTACACTTGCCGAGCTTTTCTGATTTTCGGGTGGAGGAAGTTTACTTAAAAGGTACATTGGATTTGGGATCCTTAGCAGAAATGATGGGTAGCGATAAAAGGTTTGGGCCAAAAAATCGGGCGGGAATGGGGGAGCCCTATCCCCAGTATGAGTCCCTTCAGGCAAGATTAAAGGCCTTGTCCACTTCTACCTCTGCTCAAAAGTTCAGCCTCAAAGTTTCTGATATTGGGTTGAATTCCTCCATCCCAGAGGGGGCAGCTGGAAGTATAAAGAGGTCTATTTTGTCCGAGGGTGGTGTCTTGCAAGTCTACTATGTGAAGGTTTTGGAGAAGGGAGACACATATGAGATAATTGAACGAAGCCTACCCAAGAAGCAAAAGGTACAGAAAGATCCTTCTCAGATTGAGAAGGAGGAAATGGAAAGGATGGGAAGAGTTTGGGTAAACATTGTAAGAAGAGACATACCAAAGCATCATAGAATGTTTACTACTTTTCACCGCAAGCAACTAATTGATGCCAAGAGGGTCTCAGAGAACTGTCAAAGAGAGGTAAAAATGAAGGTAAGTAGATCACTTAAAGTGATGAGGGGTGCTGCAATTCGCACAAGGAAACTAGCCAGAGACATGTTACTGCTTTGGAAGAGAATAGACAAAGAGATGGCAGAAGTGAGGAAGAAAGAGGAAAAAGAAGCTATTGAAATGCGCAAGCGTCTGGAGGAGGAAAGAGAAGCAAAGAGACAGGAGCAAAGACTCAATTTTCTCATAAAACAAACCGAACTTTACAGTCACTTCATGCAGAACAAGTTGAGTTCACAGCCATCTGAAGACTTGCCTGTAGGAGATGAAAACCAAGATGTATCTCCAAGCTCCTCAGATATTGAGAATGTTGAAGAAGATTCTGAGGAAGCTGAACTGAAGAAGGAGGCATTAAAAGCTGCTCAAGATGCAGTTTCAAAGCAGAAAAAGTTAACTAGTGCATTTGATGACGAGTGTCTGAGGCTGCGTGAAGCCTCTGAACCTGAGGCACCACAGGATGTTGCTGGAGCTAATAACATTGATCTGCATAACCCTTCCACTATGCCTGTTACATCAACCGTTCAGACACCTGAGTTGTTCAAAGGCTCCCTTAAAGAATACCAGCTAAAAGGCCTCCAGTGGCTGGTTAATTGTTATGAGCAGGGTTTAAATGGCATCCTTGCTGATGAGATGGGCCTGGGTAAGACCATCCAGGCCATGGCATTTTTGGCTCATTTGGCTGAGGAGAAAAATATATGGGGGCCTTTTCTGGTTGTTGCTCCTGCTTCTGTCTTGAACAACTGGGCTGATGAAATTAGTCGTTTCTGCCCCGACTTAAAAACTCTTCCGTATTGGGGTGGCCTTCAAGAGCGTATGGTACTTAGGAAGAAGATCAACGCTAAGAAACTTTACCGTAGGGATGCTGGGTTTCACATTCTCATTACCAGCTACCAGTTGCTAGTGGGTGATGAGAAGTGTTTCCGGCGTGTGAAATGGCAATATATGGTATTGGATGAAGCACAAGCTATCAAAAGTTCTAACAGTATAAGATGGAAGACACTGCTCAGCTTTAATTGTCGAAATCGTTTGCTGCTTACTGGTACACCTATCCAGAATAATATGGCAGAGTTGTGGGCCCTACTACATTTCATTATGCCAACCTTATTTGACAGTCATGAACAATTCAATGAGTGGTTCTCTAAAGGAATTGAAAATCATGCGGAACATGGGGGTACTTTGAACGAGCACCAGCTTAACAGATTGCATTCGATATTGAAGCCATTCATGCTCCGTCGAGTTAAGACAGATGTGATTTCTGAGCTGACCAGGAAAACTGAAGTTACTGTGCACTGCAAGTTGAGTTCTCGGCAACAAGCTTTTTATCAAGCTATCAAAAACAAGATATCTCTTGCTGAGCTGTTTGACAACAATCGTGGGCATCTTAATGAGAAGAAAATTTTGAACTTAATGAATATTGTCATTCAGCTAAGAAAGGTCTGCAACCATCCTGAGTTATTTGAGAGGAATGAAGGAAGCACATATCTTCACTTTGGTGAGATTTCAAATTCTCTTCTTCCTCCACCATTTGGGGAGTTGGAGGACGTACACTACTCGGGAGGTCAAAATCCTATAACATACGAGGTCCCCAAACTTTTATATCAAGAAATACTCCAGAATTCTGAAACGTTTTGCTCTGCAGTTCGGCATGGTGTCTACAGTGAATCTTTTCAGAAACATTTTAACATATTTTCACCGCAAAATGTGTATCGATCAATATTCTTGCAAGAGAATGACTCAGATGAGTTGTCGGTTGGAAGTGGAACGTTTGGTTTTACTCATCTGATGGATCTGTCCCCTGCAGAGGTTGCATTTTTAGGAACTGGTTCTTTTATGGAGCGGCTAATGTTTTCCATTATGAGATGGGACCGGCAGTTTTTGGATGGATTGATAGACACACTAATGGAAACCATGGATGATGATTCTGAATGCAGCTTCCTTGAGAGTGGAAAAGTGAGAGCTGTTACACGTATGTTGCTGATGCCTTCAAGATCTATAACTACCATCTTTCAGAAGAAATTGGCCACTGGACCTGGTGGTACTCCATTTGAGGGTCTAGTTGTCTCTCATCGGGACAGGCTTCTCTCTAATATTAGGCTTCTCCGTTCGACATACACATTCATCCCCCGAACTAGAGCTCCCCCGGTTAATGCCCATTGCTCAGATCGAAACTTCACTTACAAAATGAGTGAAGAACAACAGTACCCCTGGGTGAAGAGGTTGTTTTCTGGTTTTGCGCGAACATCTGACTACAATGGACCCAGAAAACCTGAGACTCCTCATCATCTGATACAAGAGATTGATTCTGAACTGCCTGTTTCACATTCAGCTCTTCAGTTGACATACAGAATTTTTGGGTCTTGTCCTCCAATGCAAAGCTTCGACCCGTCAAAAATGCTCACTGATTCGGGAAAACTTCAGACACTTGATATATTGTTGAAACGCTTGAGGGCAGAAAATCATCGTGTTCTCTTGTTTGCACAGATGACAAAGATGCTGAATATTCTTGAGGACTACATGAACTACAGGAAATATAGATACCTCAGACTCGATGGATCTTCCACCATTATGGATCGTAGAGACATGGTCAGAGACTTCCAGCAACGAAGTGATATTTTTGTTTTCTTGCTGAGTACAAGAGCTGGTGGACTAGGCATTAACTTGACAGCTGCAGATACTGTCATATTTTATGAAAGTGATTGGAATCCGACCCTGGATCTACAGGCAATGGATAGAGCTCATCGGTTGGGCCAGACAAAAGATGTTACTGTCTACCGCCTAATTTGTAAAGAAACGGTTGAGGAGAAGATTCTGCAAAGAGCAAGTCAGAAAAATACTGTGCAGCAGCTTGTAATGATGGGTGGTCATGTTCAAGGTGACCTTTTGGCTCCCGAGGATGTTGTATCTCTACTTCTCGATGATGTCCAGTTGGAGCAGAAATTAAGAGAAGCTCCATCACAGGTTAAGGATAGACAAAAGAAGAAACAGACAAAGGGTATACGGGTAGATGCAGAAGGTGATGCCTCTTTGGAAGATTTAACAAACACTGCTGCATCACAAGGTACTGGAAATGAGGAATCTCCGGATGTCGAAAGATCAAAATCCAATAATAAAAAGAGGAAAACAGTGCCTGACAAGCATACTCCGCGGCCGAAGAATCCTCAAAGTATGGATGAACCAGAGGGCTATGAATTGGAGGATTCCTTACAAAATACTGATCCACAAGGTACAAGACCCAAGAGGCCAAAGAGGTCAAAGAAGAGTGTAAATGAAACTCTTGAACCTGCATTTACAGCGGCATCCCCTGTGGTCCCGAGGCAGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBINO Helicase_C SNF2_N

Protein Analysis

1501

Amino Acids

171.67

Weight (kDa)

8.1

Isoelectric Point (pI)

53.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBINO PF13892 343 - 473 1.3e-44 DNA-binding domain
ResIII PF04851 577 - 744 1.1e-07 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 581 - 879 4e-85 SNF2-related domain
Helicase_C PF00271 1200 - 1310 2.6e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1310
Acc36I ACCTGC 1 cut(s) 4471
AccB1I GGYRCC 2 cut(s) 12, 1630
AccB7I CCANNNNNTGG 3 cut(s) 1765, 1840, 2708
AccBSI CCGCTC 1 cut(s) 3019
AccI GTMKAC 4 cut(s) 915, 2835, 3951, 4177
AccII CGCG 3 cut(s) 171, 3418, 4327
AccIII TCCGGA 1 cut(s) 4261
AclI AACGTT 2 cut(s) 2807, 2945
AclWI GGATC 9 cut(s) 164, 669, 682, 992, 2976, 3742, 3759, 3903, 4391
AcoI YGGCCR 2 cut(s) 3201, 4328
AcsI RAATTY 6 cut(s) 178, 841, 2577, 2686, 2797, 3528
AcuI CTGAAG 6 cut(s) 749, 1449, 1541, 2478, 3497, 3581
AcyI GRCGYC 1 cut(s) 13
AdeI CACNNNGTG 1 cut(s) 509
AfiI CCNNNNNNNGG 8 cut(s) 22, 859, 1765, 1840, 1976, 2708, 2983, 3230
AflIII ACRYGT 2 cut(s) 1230, 3146
AhlI ACTAGT 1 cut(s) 1577
AjiI CACGTC 1 cut(s) 438
AjnI CCWGG 7 cut(s) 472, 1818, 1833, 2447, 3211, 3386, 3891
AleI CACNNNNGTG 1 cut(s) 2673
AloI GAACNNNNNNTCC 2 cut(s) 3301, 3333
Alw21I GWGCWC 4 cut(s) 2370, 2472, 3319, 3919
Alw44I GTGCAC 1 cut(s) 2468
AlwI GGATC 9 cut(s) 164, 669, 682, 992, 2976, 3742, 3759, 3903, 4391
AlwNI CAGNNNCTG 3 cut(s) 1765, 3857, 4310
Ama87I CYCGRG 3 cut(s) 2734, 4070, 4492
Aor13HI TCCGGA 1 cut(s) 4261
ApaI GGGCCC 1 cut(s) 2260
ApaLI GTGCAC 1 cut(s) 2468
ApoI RAATTY 6 cut(s) 178, 841, 2577, 2686, 2797, 3528
ArsI GACNNNNNNTTYG 2 cut(s) 3988, 4020
Asp700I GAANNNNTTC 5 cut(s) 288, 2099, 2402, 2847, 3004
AspLEI GCGC 4 cut(s) 15, 502, 1311, 3418
AsuC2I CCSGG 1 cut(s) 3323
AsuHPI GGTGA 8 cut(s) 610, 1094, 2099, 2687, 2865, 3403, 4067, 4208
AsuII TTCGAA 1 cut(s) 380
AvaI CYCGRG 3 cut(s) 2734, 4070, 4492
AvaII GGWCC 6 cut(s) 4, 2764, 3046, 3209, 3437, 4489
AxyI CCTNAGG 1 cut(s) 1626
BaeGI GKGCMC 2 cut(s) 2260, 2472
BaeI ACNNNNGTAYC 2 cut(s) 3365, 3398
BalI TGGCCA 1 cut(s) 3203
BamHI GGATCC 1 cut(s) 674
BanI GGYRCC 2 cut(s) 12, 1630
BanII GRGCYC 4 cut(s) 744, 2260, 3319, 3919
BarI GAAGNNNNNNTAC 2 cut(s) 4074, 4106
BbsI GAAGAC 2 cut(s) 1437, 2183
Bbv12I GWGCWC 4 cut(s) 2370, 2472, 3319, 3919
BbvCI CCTCAGC 1 cut(s) 1863
BceAI ACGGC 1 cut(s) 115
BciT130I CCWGG 7 cut(s) 474, 1820, 1835, 2449, 3213, 3388, 3893
BcnI CCSGG 1 cut(s) 3323
BcuI ACTAGT 1 cut(s) 1577
BfaI CTAG 6 cut(s) 1220, 1578, 2081, 3236, 3312, 3830
BfmI CTRYAG 7 cut(s) 1440, 2817, 2836, 2979, 3703, 3849, 3899
BfoI RGCGCY 1 cut(s) 16
BfuAI ACCTGC 1 cut(s) 4471
BglII AGATCT 1 cut(s) 3170
BlpI GCTNAGC 2 cut(s) 2186, 2535
Bme1390I CCNGG 8 cut(s) 474, 1820, 1835, 2449, 3213, 3323, 3388, 3893
Bme18I GGWCC 6 cut(s) 4, 2764, 3046, 3209, 3437, 4489
BmeT110I CYCGRG 3 cut(s) 2734, 4070, 4492
BmgBI CACGTC 1 cut(s) 438
BmrFI CCNGG 8 cut(s) 474, 1820, 1835, 2449, 3213, 3323, 3388, 3893
BmrI ACTGGG 2 cut(s) 745, 1933
BmuI ACTGGG 2 cut(s) 745, 1933
BoxI GACNNNNGTC 1 cut(s) 1596
BpiI GAAGAC 2 cut(s) 1437, 2183
BplI GAGNNNNNCTC 6 cut(s) 187, 219, 2303, 2335, 2900, 2932
BpmI CTGGAG 4 cut(s) 1340, 1668, 1742, 2777
Bpu10I CCTNAGC 2 cut(s) 679, 1863
Bpu1102I GCTNAGC 2 cut(s) 2186, 2535
Bpu14I TTCGAA 1 cut(s) 380
BpuEI CTTGAG 5 cut(s) 83, 1530, 3140, 3646, 3707
BpuMI CCSGG 1 cut(s) 3323
Bsa29I ATCGAT 1 cut(s) 2890
BsaAI YACGTR 1 cut(s) 3149
BsaBI GATNNNNATC 2 cut(s) 3882, 4279
BsaHI GRCGYC 1 cut(s) 13
BsaI GGTCTC 2 cut(s) 207, 1138
BsaWI WCCGGW 2 cut(s) 355, 4261
BsaXI ACNNNNNCTCC 2 cut(s) 3301, 3331
Bsc4I CCNNNNNNNGG 8 cut(s) 22, 859, 1765, 1840, 1976, 2708, 2983, 3230
Bse118I RCCGGY 1 cut(s) 3048
Bse21I CCTNAGG 1 cut(s) 1626
Bse3DI GCAATG 2 cut(s) 3334, 3912
Bse8I GATNNNNATC 2 cut(s) 3882, 4279
BseAI TCCGGA 1 cut(s) 4261
BseBI CCWGG 7 cut(s) 474, 1820, 1835, 2449, 3213, 3388, 3893
BseCI ATCGAT 1 cut(s) 2890
BseJI GATNNNNATC 2 cut(s) 3882, 4279
BseLI CCNNNNNNNGG 8 cut(s) 22, 859, 1765, 1840, 1976, 2708, 2983, 3230
BseMI GCAATG 2 cut(s) 3334, 3912
BseRI GAGGAG 9 cut(s) 25, 28, 236, 426, 1337, 1465, 1880, 3447, 3995
BseSI GKGCMC 2 cut(s) 2260, 2472
BseX3I CGGCCG 1 cut(s) 4328
BseYI CCCAGC 1 cut(s) 2045
BsgI GTGCAG 2 cut(s) 318, 4040
Bsh1236I CGCG 3 cut(s) 171, 3418, 4327
Bsh1285I CGRYCG 2 cut(s) 169, 4331
BshNI GGYRCC 2 cut(s) 12, 1630
BshVI ATCGAT 1 cut(s) 2890
BsiEI CGRYCG 2 cut(s) 169, 4331
BsiHKAI GWGCWC 4 cut(s) 2370, 2472, 3319, 3919
BsiHKCI CYCGRG 3 cut(s) 2734, 4070, 4492
BsiSI CCGG 5 cut(s) 356, 2104, 3049, 3323, 4262
BslFI GGGAC 7 cut(s) 256, 745, 2750, 2959, 3059, 3266, 4475
BslI CCNNNNNNNGG 8 cut(s) 22, 859, 1765, 1840, 1976, 2708, 2983, 3230
BsmFI GGGAC 7 cut(s) 256, 745, 2750, 2959, 3059, 3266, 4475
BsmI GAATGC 2 cut(s) 2386, 3113
Bso31I GGTCTC 2 cut(s) 207, 1138
BsoBI CYCGRG 3 cut(s) 2734, 4070, 4492
Bsp119I TTCGAA 1 cut(s) 380
Bsp120I GGGCCC 1 cut(s) 2256
Bsp1286I GDGCHC 6 cut(s) 744, 2260, 2370, 2472, 3319, 3919
Bsp13I TCCGGA 1 cut(s) 4261
Bsp1720I GCTNAGC 2 cut(s) 2186, 2535
Bsp19I CCATGG 2 cut(s) 1839, 3090
BspDI ATCGAT 1 cut(s) 2890
BspEI TCCGGA 1 cut(s) 4261
BspFNI CGCG 3 cut(s) 171, 3418, 4327
BspHI TCATGA 1 cut(s) 2296
BspMAI CTGCAG 3 cut(s) 2821, 2983, 3853
BspMI ACCTGC 1 cut(s) 4471
BspPI GGATC 9 cut(s) 164, 669, 682, 992, 2976, 3742, 3759, 3903, 4391
BspQI GCTCTTC 1 cut(s) 3516
BspT104I TTCGAA 1 cut(s) 380
BspT107I GGYRCC 2 cut(s) 12, 1630
BspTNI GGTCTC 2 cut(s) 207, 1138
BsrBI CCGCTC 1 cut(s) 3019
BsrDI GCAATG 2 cut(s) 3334, 3912
BsrFI RCCGGY 1 cut(s) 3048
BssAI RCCGGY 1 cut(s) 3048
BssNAI GTATAC 1 cut(s) 4178
BssNI GRCGYC 1 cut(s) 13
BssT1I CCWWGG 4 cut(s) 21, 133, 1839, 3090
Bst1107I GTATAC 1 cut(s) 4178
Bst2UI CCWGG 7 cut(s) 474, 1820, 1835, 2449, 3213, 3388, 3893
Bst6I CTCTTC 9 cut(s) 59, 1033, 1057, 1241, 1971, 2697, 3389, 3516, 4433
BstACI GRCGYC 1 cut(s) 13
BstAPI GCANNNNNTGC 1 cut(s) 2987
BstBAI YACGTR 1 cut(s) 3149
BstBI TTCGAA 1 cut(s) 380
BstC8I GCNNGC 3 cut(s) 396, 1109, 1313
BstDSI CCRYGG 3 cut(s) 1839, 3090, 4325
BstEII GGTNACC 1 cut(s) 4055
BstFNI CGCG 3 cut(s) 171, 3418, 4327
BstH2I RGCGCY 1 cut(s) 16
BstHHI GCGC 4 cut(s) 15, 502, 1311, 3418
BstMCI CGRYCG 2 cut(s) 169, 4331
BstNI CCWGG 7 cut(s) 474, 1820, 1835, 2449, 3213, 3388, 3893
BstNSI RCATGY 1 cut(s) 1234
BstPAI GACNNNNGTC 1 cut(s) 1596
BstPI GGTNACC 1 cut(s) 4055
BstSCI CCNGG 8 cut(s) 472, 1818, 1833, 2447, 3211, 3321, 3386, 3891
BstSFI CTRYAG 7 cut(s) 1440, 2817, 2836, 2979, 3703, 3849, 3899
BstSLI GKGCMC 2 cut(s) 2260, 2472
BstUI CGCG 3 cut(s) 171, 3418, 4327
BstV2I GAAGAC 2 cut(s) 1437, 2183
BstX2I RGATCY 6 cut(s) 674, 997, 2968, 3170, 3734, 3895
BstXI CCANNNNNNTGG 1 cut(s) 3748
BstYI RGATCY 6 cut(s) 674, 997, 2968, 3170, 3734, 3895
BstZ17I GTATAC 1 cut(s) 4178
BstZI CGGCCG 1 cut(s) 4328
Bsu15I ATCGAT 1 cut(s) 2890
Bsu36I CCTNAGG 1 cut(s) 1626
BsuTUI ATCGAT 1 cut(s) 2890
BtgI CCRYGG 3 cut(s) 1839, 3090, 4325
BtrI CACGTC 1 cut(s) 438
BtsI GCAGTG 3 cut(s) 2180, 2470, 4224
BtsIMutI CAGTG 9 cut(s) 217, 1766, 2180, 2470, 2845, 3204, 3579, 4224, 4311
BveI ACCTGC 1 cut(s) 4471
Cac8I GCNNGC 3 cut(s) 396, 1109, 1313
CaiI CAGNNNCTG 3 cut(s) 1765, 3857, 4310
CciI TCATGA 1 cut(s) 2296
CfoI GCGC 4 cut(s) 15, 502, 1311, 3418
Cfr10I RCCGGY 1 cut(s) 3048
Cfr42I CCGCGG 1 cut(s) 4328
ClaI ATCGAT 1 cut(s) 2890
CseI GACGC 2 cut(s) 253, 1304
CsiI ACCWGGT 1 cut(s) 3211
CspCI CAANNNNNGTGG 2 cut(s) 2536, 2571
DinI GGCGCC 1 cut(s) 14
DraI TTTAAA 1 cut(s) 1791
DraIII CACNNNGTG 1 cut(s) 509
EaeI YGGCCR 2 cut(s) 3201, 4328
EagI CGGCCG 1 cut(s) 4328
Eam1104I CTCTTC 9 cut(s) 59, 1033, 1057, 1241, 1971, 2697, 3389, 3516, 4433
EarI CTCTTC 9 cut(s) 59, 1033, 1057, 1241, 1971, 2697, 3389, 3516, 4433
Ecl136II GAGCTC 2 cut(s) 3317, 3917
EclXI CGGCCG 1 cut(s) 4328
Eco130I CCWWGG 4 cut(s) 21, 133, 1839, 3090
Eco147I AGGCCT 2 cut(s) 782, 1755
Eco24I GRGCYC 4 cut(s) 744, 2260, 3319, 3919
Eco31I GGTCTC 2 cut(s) 207, 1138
Eco32I GATATC 1 cut(s) 2526
Eco47I GGWCC 6 cut(s) 4, 2764, 3046, 3209, 3437, 4489
Eco52I CGGCCG 1 cut(s) 4328
Eco53kI GAGCTC 2 cut(s) 3317, 3917
Eco57I CTGAAG 6 cut(s) 749, 1449, 1541, 2478, 3497, 3581
Eco81I CCTNAGG 1 cut(s) 1626
Eco88I CYCGRG 3 cut(s) 2734, 4070, 4492
Eco91I GGTNACC 1 cut(s) 4055
EcoICRI GAGCTC 2 cut(s) 3317, 3917
EcoO109I RGGNCCY 3 cut(s) 1883, 2257, 2764
EcoO65I GGTNACC 1 cut(s) 4055
EcoRI GAATTC 3 cut(s) 178, 841, 2797
EcoRII CCWGG 7 cut(s) 472, 1818, 1833, 2447, 3211, 3386, 3891
EcoRV GATATC 1 cut(s) 2526
EcoT14I CCWWGG 4 cut(s) 21, 133, 1839, 3090
EcoT38I GRGCYC 4 cut(s) 744, 2260, 3319, 3919
EgeI GGCGCC 1 cut(s) 14
EheI GGCGCC 1 cut(s) 14
ErhI CCWWGG 4 cut(s) 21, 133, 1839, 3090
FalI AAGNNNNNCTT 4 cut(s) 1716, 1748, 4044, 4076
FaqI GGGAC 7 cut(s) 256, 745, 2750, 2959, 3059, 3266, 4475
FauI CCCGC 1 cut(s) 721
FauNDI CATATG 1 cut(s) 949
FblI GTMKAC 4 cut(s) 915, 2835, 3951, 4177
FriOI GRGCYC 4 cut(s) 744, 2260, 3319, 3919
FspBI CTAG 6 cut(s) 1220, 1578, 2081, 3236, 3312, 3830
FspI TGCGCA 1 cut(s) 1310
GlaI GCGC 4 cut(s) 14, 501, 1310, 3417
GsaI CCCAGC 1 cut(s) 2049
GsuI CTGGAG 4 cut(s) 1340, 1668, 1742, 2777
HaeII RGCGCY 1 cut(s) 16
HapII CCGG 5 cut(s) 356, 2104, 3049, 3323, 4262
HgaI GACGC 2 cut(s) 253, 1304
HhaI GCGC 4 cut(s) 15, 502, 1311, 3418
Hin1I GRCGYC 1 cut(s) 13
Hin6I GCGC 4 cut(s) 13, 500, 1309, 3416
HinP1I GCGC 4 cut(s) 13, 500, 1309, 3416
HincII GTYRAC 2 cut(s) 1575, 3519
HindII GTYRAC 2 cut(s) 1575, 3519
HindIII AAGCTT 2 cut(s) 2496, 3556
HpaI GTTAAC 1 cut(s) 1575
HpaII CCGG 5 cut(s) 356, 2104, 3049, 3323, 4262
HphI GGTGA 8 cut(s) 610, 1094, 2099, 2687, 2865, 3403, 4067, 4208
Hpy99I CGWCG 1 cut(s) 2418
HpyCH4IV ACGT 5 cut(s) 437, 2724, 2807, 2945, 3148
HpySE526I ACGT 5 cut(s) 437, 2724, 2807, 2945, 3148
Hsp92I GRCGYC 1 cut(s) 13
HspAI GCGC 4 cut(s) 13, 500, 1309, 3416
KasI GGCGCC 1 cut(s) 12
Kpn2I TCCGGA 1 cut(s) 4261
KspAI GTTAAC 1 cut(s) 1575
KspI CCGCGG 1 cut(s) 4328
LguI GCTCTTC 1 cut(s) 3516
MabI ACCWGGT 1 cut(s) 3211
MaeI CTAG 6 cut(s) 1220, 1578, 2081, 3236, 3312, 3830
MaeII ACGT 5 cut(s) 437, 2724, 2807, 2945, 3148
MaeIII GTNAC 8 cut(s) 598, 1233, 1391, 1690, 2461, 3142, 3943, 4055
MbiI CCGCTC 1 cut(s) 3019
MflI RGATCY 6 cut(s) 674, 997, 2968, 3170, 3734, 3895
MhlI GDGCHC 6 cut(s) 744, 2260, 2370, 2472, 3319, 3919
MlsI TGGCCA 1 cut(s) 3203
MluNI TGGCCA 1 cut(s) 3203
Mly113I GGCGCC 1 cut(s) 13
MlyI GAGTC 5 cut(s) 767, 1350, 2909, 3448, 3720
MmeI TCCRAC 6 cut(s) 72, 189, 2697, 2914, 3911, 4089
Mox20I TGGCCA 1 cut(s) 3203
MroI TCCGGA 1 cut(s) 4261
MroXI GAANNNNTTC 5 cut(s) 288, 2099, 2402, 2847, 3004
MscI TGGCCA 1 cut(s) 3203
MslI CAYNNNNRTG 4 cut(s) 2565, 2673, 3080, 3746
Msp20I TGGCCA 1 cut(s) 3203
MspA1I CMGCKG 5 cut(s) 515, 866, 3848, 4327, 4475
MspI CCGG 5 cut(s) 356, 2104, 3049, 3323, 4262
MspR9I CCNGG 8 cut(s) 474, 1820, 1835, 2449, 3213, 3323, 3388, 3893
Mva1269I GAATGC 2 cut(s) 2386, 3113
MvaI CCWGG 7 cut(s) 474, 1820, 1835, 2449, 3213, 3388, 3893
MvnI CGCG 3 cut(s) 171, 3418, 4327
NarI GGCGCC 1 cut(s) 13
NciI CCSGG 1 cut(s) 3323
NcoI CCATGG 2 cut(s) 1839, 3090
NdeI CATATG 1 cut(s) 949
NmeAIII GCCGAG 2 cut(s) 642, 2467
NmuCI GTSAC 3 cut(s) 598, 1391, 4055
NsbI TGCGCA 1 cut(s) 1310
NspI RCATGY 1 cut(s) 1234
NspV TTCGAA 1 cut(s) 380
OliI CACNNNNGTG 1 cut(s) 2673
PagI TCATGA 1 cut(s) 2296
PasI CCCWGGG 1 cut(s) 3387
PceI AGGCCT 2 cut(s) 782, 1755
PciI ACATGT 1 cut(s) 1230
PciSI GCTCTTC 1 cut(s) 3516
PctI GAATGC 2 cut(s) 2386, 3113
PdmI GAANNNNTTC 5 cut(s) 288, 2099, 2402, 2847, 3004
PflFI GACNNNGTC 1 cut(s) 3764
PflMI CCANNNNNTGG 3 cut(s) 1765, 1840, 2708
PleI GAGTC 5 cut(s) 766, 1350, 2909, 3448, 3720
PluTI GGCGCC 1 cut(s) 16
PpsI GAGTC 5 cut(s) 766, 1350, 2909, 3448, 3720
Ppu21I YACGTR 1 cut(s) 3149
PpuMI RGGWCCY 1 cut(s) 2764
PscI ACATGT 1 cut(s) 1230
PshAI GACNNNNGTC 1 cut(s) 1596
Psp124BI GAGCTC 2 cut(s) 3319, 3919
Psp1406I AACGTT 2 cut(s) 2807, 2945
Psp5II RGGWCCY 1 cut(s) 2764
Psp6I CCWGG 7 cut(s) 472, 1818, 1833, 2447, 3211, 3386, 3891
PspEI GGTNACC 1 cut(s) 4055
PspFI CCCAGC 1 cut(s) 2045
PspGI CCWGG 7 cut(s) 472, 1818, 1833, 2447, 3211, 3386, 3891
PspOMI GGGCCC 1 cut(s) 2256
PspPPI RGGWCCY 1 cut(s) 2764
PsrI GAACNNNNNNTAC 4 cut(s) 361, 393, 954, 986
PstI CTGCAG 3 cut(s) 2821, 2983, 3853
PstNI CAGNNNCTG 3 cut(s) 1765, 3857, 4310
PsuI RGATCY 6 cut(s) 674, 997, 2968, 3170, 3734, 3895
PsyI GACNNNGTC 1 cut(s) 3764
PvuII CAGCTG 2 cut(s) 866, 3848
RseI CAYNNNNRTG 4 cut(s) 2565, 2673, 3080, 3746
SacI GAGCTC 2 cut(s) 3319, 3919
SacII CCGCGG 1 cut(s) 4328
SapI GCTCTTC 1 cut(s) 3516
SchI GAGTC 5 cut(s) 767, 1350, 2909, 3448, 3720
ScrFI CCNGG 8 cut(s) 474, 1820, 1835, 2449, 3213, 3323, 3388, 3893
SduI GDGCHC 6 cut(s) 744, 2260, 2370, 2472, 3319, 3919
SexAI ACCWGGT 1 cut(s) 3211
SfcI CTRYAG 7 cut(s) 1440, 2817, 2836, 2979, 3703, 3849, 3899
SfoI GGCGCC 1 cut(s) 14
Sfr303I CCGCGG 1 cut(s) 4328
SfuI TTCGAA 1 cut(s) 380
SgrBI CCGCGG 1 cut(s) 4328
SinI GGWCC 6 cut(s) 4, 2764, 3046, 3209, 3437, 4489
SmiMI CAYNNNNRTG 4 cut(s) 2565, 2673, 3080, 3746
SmlI CTYRAG 5 cut(s) 62, 1545, 3119, 3625, 3686
SmoI CTYRAG 5 cut(s) 62, 1545, 3119, 3625, 3686
SpeI ACTAGT 1 cut(s) 1577
SseBI AGGCCT 2 cut(s) 782, 1755
SspDI GGCGCC 1 cut(s) 12
SspI AATATT 4 cut(s) 2596, 2898, 3271, 3682
SspMI CTAG 6 cut(s) 1220, 1578, 2081, 3236, 3312, 3830
SstI GAGCTC 2 cut(s) 3319, 3919
StuI AGGCCT 2 cut(s) 782, 1755
StyD4I CCNGG 8 cut(s) 472, 1818, 1833, 2447, 3211, 3321, 3386, 3891
StyI CCWWGG 4 cut(s) 21, 133, 1839, 3090
TaiI ACGT 5 cut(s) 440, 2727, 2810, 2948, 3151
TatI WGTACW 2 cut(s) 297, 3812
TauI GCSGC 4 cut(s) 515, 3022, 4330, 4478
TscAI CASTG 9 cut(s) 217, 1766, 2187, 2477, 2845, 3211, 3586, 4231, 4311
TseFI GTSAC 3 cut(s) 598, 1391, 4055
Tsp45I GTSAC 3 cut(s) 598, 1391, 4055
TspGWI ACGGA 3 cut(s) 1959, 2402, 3272
TspRI CASTG 9 cut(s) 217, 1766, 2187, 2477, 2845, 3211, 3586, 4231, 4311
Tth111I GACNNNGTC 1 cut(s) 3764
Van91I CCANNNNNTGG 3 cut(s) 1765, 1840, 2708
VneI GTGCAC 1 cut(s) 2468
VpaK11BI GGWCC 6 cut(s) 4, 2764, 3046, 3209, 3437, 4489
XapI RAATTY 6 cut(s) 178, 841, 2577, 2686, 2797, 3528
XceI RCATGY 1 cut(s) 1234
XcmI CCANNNNNNNNNTGG 2 cut(s) 1837, 1846
XmiI GTMKAC 4 cut(s) 915, 2835, 3951, 4177
XmnI GAANNNNTTC 5 cut(s) 288, 2099, 2402, 2847, 3004
XspI CTAG 6 cut(s) 1220, 1578, 2081, 3236, 3312, 3830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.