MD11G1158800.v1.1

DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005736
Physical Location & Seq
Reverse (-)
15443654 .. 15457805
14152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1158800.v1.1.491

Sequence Viewer

Length: 4530 bp
ATGGACCACCGGAAGCAACCCAAGGATTCTCTCTCCTACTCCAACCTTTTCAATCTTGAGTCTTTGATGAACTTTCAAGTTCCCCGACCGGATGACGATTTCGATTATTATGGAAATAGTAGTCAGGATGAGAGCAGAGGTAGCCAAGATGGAGCTGTAGGTAATGGGATGATGTCTGACAGAGAACTGAGTTCAGTGAAAAAAAGAAGGCGATCCCAAAATAGTGACTACGAGGACGATGACAGTTATTACGGGACACGCATTACAGAGGAAAGGTATCGATCAATGCTTGGAGAACACATCCAGAAGTACAAAAGGAGGTTCAAGGATTCCTCGTCGAGTCCTGCACCAATTCAGACGGGGATTCCAGTTCCAAAGAGTAATAAGGGTTTAAAATCAAGGAAACTGGGCAATGAGCACCGGGGAGGGATATATGAAATGGAAACCTCATCAGAGTGGCTCAATGATTCTAATCCTCAAAAACCCGGGAACCACCATGATGGAGATTTGGTACCACAACGTGGCACCGATAGAATAATCTATGAACCTCCTTATCTGGATATAGGAGACGGTATCACTTACAGGATTCCACCAATTTACGATAAGCTGGTGTCGTCTTTGCACTTACCGAGCTTTTCAGATTTTCGGGTGGAGGAAGTTTACTTAAGAGGCACATTGGACTTGGGATCCTTGGCAGAAATGATGACTAGTGACAAAAGGTTGGGTCCTAAAAACCAGGCAGGGATGGGTGATCCCCATCCCCAGTATGAATCCCTTCAAGCTAGACTAAAGGCATCGTCCACTTCAAATTCAGCCCAAAAGTTCAGCCTCAAGGTCTCTGATATTGGGTTAAACTCTTCCATCCCAGAGGGTGCAGCTGGAAATATAAAGAGATCTATTTTGTCTGAAGGTGGAGTTTTACAGGTCTACTATGTGAAAGTTTTGGAGAAAGGAGACACGTATGAGATCATTGAAAGAAGTCTACCGAAGAAGCAAAAGGTTATTAAAGACCCTTCTGTGATTGAGAAGGAGGAAATGGACAAGATTGGGAAAGTTTGGGTAAACATAGTAAGAAGAGACATGCCAAGGCATCATAGGATTTTTTCAAATTTTCACCAGAGGCAACTAGGTTATGCCAAGAGGGTCTCAGAGAACTGTCAAAGAGAGGTGAAATTGAAGGTCAGTAGATCTCTTAAACTGATGAGGGGTGCCGCAATTCGTACAAGGAAACTAGCCAGAGATATGCTACTATTTTGGAAGAGAATAGATAAAGAGATGGCGGAAGTGAGGAAAAAAGAGGAGAAAGAAGCAGCTGAAGCTTTGAGGCGCGAACAGGAGCTTCGAGAAGCAAAGAGACAGCAGCAAAGGCTCAATTTTCTTATACAACAGACGGAACTTTACAGTCACTTTATGCAGAACAAGTCAAGTTCACAGCCGTCAGAAGATCTGCCTGTTGGTGATGGAAAAAAAATGGAGGAAAAAGCATCTTTAAGCTCCTCAGATGATGAAGCTAATGAGGAAGAAGACCCTGAGGAAGCTGAACTGAAGAAGGAGGCTTTTAAAGCAGCTCAAGATGCAGTTTCAAAGCAGAAAAAGATAACTAGTGAATTTGATAATGAGTATTTGAAGCTATGTGAAGATGCTGAACCTGAGGCAGCACATGATGTTGCTGGAGCTAGTAGCATAGATCTACATAACCCTTCCACAATGCCTGTAACATCAACTGTTCAGACACCGGAGTTGTTCAAAGGATCCCTTAAAGAATATCAGCTGAAAGGCCTCCAGTGGTTGGTTAATTGCTATGAGCAGGGTTTAAATGGCATCCTTGCTGATGAGATGGGCCTAGGAAAGACCATCCAGGCCATGGCATTTTTGGCTCATTTGGCTGAGGAGAAAAATATTTGGGGGCCTTTTCTGGTTGTTGCTCCTGCCTCTGTCTTGAACAACTGGGCAGATGAAATTAGTCGTTTCTGCCCTGACTTAAAAACTCTTCCATACTGGGGAGGACTTAACGAGCGTCAGGTACTTAGGAAAAAGATCACTGCTAAAAAACTTTACCGCAGGGATGCTGGATTTCATATTCTCATTACCAGCTACCAGCTGCTAGTGGCTGATGAAAAGTATTTTAAACGTGTGAAATGGCAGTATATGGTATTAGATGAAGCCCAAGCAATCAAAAGTTCTAACAGCATAAGATGGAAGACACTTCTAGGCTTTAATTGTCGAAATCGTTTGCTACTTACCGGTACACCTATCCAAAATAATATGGCAGAGTTGTGGGCTCTACTACATTTCATTATGCCAACGTTGTTTGACAGCCATGAACAATTCAATGAGTGGTTTTCAAAAGGAATTGAGAACCATGCGGAACATGGGGGTACTTTGAATGAGCACCAGCTTAACAGATTGCATTCCATTTTAAAGCCATTCATGCTGCGCCGAGTTAAAACAGATGTGGTTTCTGAGCTGACCAGGAAAACTGAGGTTACCGTGCACTGCAAGTTGAGCTCTCGGCAACAAGATTTTTATCAAGCTATCAAGAACAAAATATCTCTTGCTGAGTTGTTTGACAGCAATCGCGGTCATCTTAATGAGAAGAAAATTTTGAATTTGATGAATATTGTCATTCAGCTGAGAAAGGTCTGCAACCATCCTGAGTTATTTGAGAGGAATGAAGGAAGTACATATCTGTACTTTAGTGAGATACCAAATTCTCTTCTAGCTCCTCCATTTGGGGAGTTGGAGGACGTGCACTACTCAGGAGGTCAAAATCCTATAACATATTCGATCCCTAAACTTTTCTATCAAGAAATTCTCCAGAGCTCTGAAACTTTGTGCTCAGCAGTTCAACATGGTGTTTACAAAGAATCTTTTGAGAAATATTTTAACATATTTTCACCGGGAAATGTTTATCAATCGACACTCTTGCAAGAGAATAGCTCATCTGTTGGAAGTGGAACTTTTGGCTTTACTCGTATGATGGATCTGTCCCCTGCTGAGGTGGCATTTCTAGGAACTGGTTCTTTTATGGAGCGACTGATGTTTTCCATTGTGAGATGGGACCGACAATTTTTGGATGGGATTGTGGACTCACTAATGGAAACCATGAATAATGATTCTGAATGCAGTTATCTTGAGAGTGGAAAAGTGAGAGCTGTTACACGAATGTTGTTGATGCCTTCAAGATCTGTAACTAACTTTCTTCAAAAGAAATTGGCTACAGGCCCTGGTGATGATCCATTCGAGGCTTTAGTTGTCTCTCATAGTGACAGGCTACTCTCTAACACTAGGCTTCTTCATTCAGCATACACATTCATCCCACGAACTAGAGCTCCCCCGGTCAATGCCCATTGCTCAGACAGAAACTTTGCTTACAAAATAATTGAAGAACTACAATACCCTTGGGTGAAGAGGTTGTTTACTGGGTTTGCACGCACATCTGACTTTAATGGACCCAGAAAACCTGAGACTCCACATCATTTAATTCAAGAGATTGATTCTGAACTGACTGTTTCATGTCCGGCTCTTCAGTTAACGTACAAGATTTTTGGTTCTTGTCCTCCTATGCAAAGCTTTGACCCAGCAAAATTGCTCACCGATTCTGGGAAACTTCAGACGCTTGATATACTGTTGAAACGCTTGAGGGCGGACAACCATCGTGTTCTCTTGTTTGCTCAAATGACAAAGATGCTGAATATTCTTGAGGACTACATGAACTACAGGAAATATAAATACTTACGACTTGATGGATCTTCCACCATTATGGATCGGCGAGACATGGTCAGAGACTTTCAGCAACGGAGTGATATTTTTGTGTTCTTGTTGAGTACAAGAGCTGGTGGACTTGGCATTAACTTGACAGCGGCTGATACTGTCATATTTTATGAAAGTGATTGGAATCCAACCCTGGATCTACAGGCAATGGATAGAGCTCATCGGCTGGGTCAGACAAAAGATGTCACCGTGTACCGACTTATTTGTAAAGAAACAGTTGAAGAGAAGATCCTGCAAAGAGCAAGTCAGAAAAATACTGTCCAGCAGCTTGTAATGATGGGTGGTCATGTTCAGGGTGATATTTTGGCTCCTGAGGATGTTGTATCTTTACTTCTGGATGATGCTCAGTTGGAGCAGAAATTAAGAGAAATTCCGTTACAGGTTAAGGATAGACAAAAGAAGAAACAAACAAAGGGTATACGAGTGGATGCAGAAGGTGATGCCTCTTTAGAAGATTTAACAAACCCTGCATCTGCGCCTCAGGGAACCGGATATGAGGACTCTCCAGATATGGAAAAAGCAAAATCTAATAATAAAAAGAGGAAAGTGGCAACGGGCAAGCAAACTACAAGGCCGAAGAATTCACAAAGCATGGACGAACTTGATTCATATGAATTGGATGATACCTTACCAAATACTGATCCACAAGCTACGAAACCCAAGAGGCCGAAGAGGTCAAAGAAGAGTGTGAACGAAAATCTTGAGCCGGCATTTACTGCAGTTTCGGAACAGACCCAGTATCCACCACGGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBINO Helicase_C SNF2_N

Protein Analysis

1510

Amino Acids

172.25

Weight (kDa)

8.08

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBINO PF13892 347 - 477 4.1e-46 DNA-binding domain
ResIII PF04851 585 - 752 3.3e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 589 - 887 1.2e-85 SNF2-related domain
Helicase_C PF00271 1205 - 1315 2.7e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 511
AccB1I GGYRCC 3 cut(s) 511, 524, 1208
AccB7I CCANNNNNTGG 3 cut(s) 521, 1789, 1864
AccI GTMKAC 3 cut(s) 927, 982, 4192
AccII CGCG 2 cut(s) 1329, 2580
AciI CCGC 7 cut(s) 1212, 1280, 2059, 2366, 2580, 3647, 3863
AclI AACGTT 1 cut(s) 2306
AcsI RAATTY 9 cut(s) 808, 1108, 1607, 2601, 2608, 2710, 2811, 4143, 4354
AcuI CTGAAG 5 cut(s) 927, 1335, 1565, 3512, 3596
AdeI CACNNNGTG 1 cut(s) 521
AfiI CCNNNNNNNGG 7 cut(s) 521, 1789, 1864, 2000, 2732, 2998, 3603
AflII CTTAAG 1 cut(s) 664
AflIII ACRYGT 2 cut(s) 957, 2131
AgeI ACCGGT 1 cut(s) 2243
AhlI ACTAGT 2 cut(s) 707, 1601
AjiI CACGTC 1 cut(s) 2749
AjnI CCWGG 5 cut(s) 735, 1857, 2471, 3226, 3906
AloI GAACNNNNNNTCC 2 cut(s) 3316, 3348
Alw21I GWGCWC 9 cut(s) 420, 2394, 2496, 2510, 2754, 2825, 2840, 3334, 3934
Alw44I GTGCAC 2 cut(s) 2492, 2750
AlwNI CAGNNNCTG 2 cut(s) 3227, 3866
Ama87I CYCGRG 1 cut(s) 485
AoxI GGCC 7 cut(s) 1777, 1840, 1860, 1907, 3223, 4346, 4439
ApaLI GTGCAC 2 cut(s) 2492, 2750
ApeKI GCWGC 8 cut(s) 875, 1310, 1360, 1565, 1655, 2101, 2434, 4039
ApoI RAATTY 9 cut(s) 808, 1108, 1607, 2601, 2608, 2710, 2811, 4143, 4354
ArsI GACNNNNNNTTYG 4 cut(s) 3350, 3382, 4003, 4035
AsiGI ACCGGT 1 cut(s) 2243
Asp700I GAANNNNTTC 2 cut(s) 774, 3019
Asp718I GGTACC 1 cut(s) 511
AspA2I CCTAGG 1 cut(s) 1843
AspLEI GCGC 3 cut(s) 1329, 2439, 4252
AspS9I GGNCC 7 cut(s) 4, 725, 1840, 1907, 3061, 3224, 3452
AsuC2I CCSGG 5 cut(s) 422, 486, 487, 2901, 3338
AvaI CYCGRG 1 cut(s) 485
AvaII GGWCC 4 cut(s) 4, 725, 3061, 3452
AvrII CCTAGG 1 cut(s) 1843
AxyI CCTNAGG 4 cut(s) 1530, 1650, 4086, 4254
BaeGI GKGCMC 2 cut(s) 2496, 2754
BamHI GGATCC 2 cut(s) 686, 1751
BanI GGYRCC 3 cut(s) 511, 524, 1208
BanII GRGCYC 5 cut(s) 2284, 2510, 2825, 3334, 3934
BarI GAAGNNNNNNTAC 2 cut(s) 4089, 4121
BbsI GAAGAC 2 cut(s) 1530, 2207
Bbv12I GWGCWC 9 cut(s) 420, 2394, 2496, 2510, 2754, 2825, 2840, 3334, 3934
BbvCI CCTCAGC 2 cut(s) 1887, 2997
BbvI GCAGC 8 cut(s) 887, 1322, 1372, 1577, 1667, 2088, 2421, 4051
BceAI ACGGC 1 cut(s) 1420
BcgI CGANNNNNNTGC 2 cut(s) 2908, 2942
BciT130I CCWGG 5 cut(s) 737, 1859, 2473, 3228, 3908
BciVI GTATCC 1 cut(s) 4524
BcnI CCSGG 5 cut(s) 422, 486, 487, 2901, 3338
BcuI ACTAGT 2 cut(s) 707, 1601
BfmI CTRYAG 5 cut(s) 156, 3219, 3718, 3914, 4491
BfrI CTTAAG 1 cut(s) 664
BfuI GTATCC 1 cut(s) 4524
BglII AGATCT 5 cut(s) 893, 1187, 1444, 1687, 3185
BlnI CCTAGG 1 cut(s) 1843
BlpI GCTNAGC 1 cut(s) 2839
Bme18I GGWCC 4 cut(s) 4, 725, 3061, 3452
BmeT110I CYCGRG 1 cut(s) 485
BmgBI CACGTC 1 cut(s) 2749
BmgT120I GGNCC 7 cut(s) 4, 725, 1840, 1907, 3061, 3224, 3452
BmrI ACTGGG 6 cut(s) 416, 757, 1957, 2008, 3432, 4504
BmuI ACTGGG 6 cut(s) 416, 757, 1957, 2008, 3432, 4504
BpiI GAAGAC 2 cut(s) 1530, 2207
BplI GAGNNNNNCTC 2 cut(s) 2924, 2956
BpmI CTGGAG 4 cut(s) 1692, 1766, 2801, 4263
Bpu10I CCTNAGC 2 cut(s) 1887, 2997
Bpu1102I GCTNAGC 1 cut(s) 2839
BpuEI CTTGAG 7 cut(s) 77, 815, 1554, 3155, 3661, 3722, 4496
BpuMI CCSGG 5 cut(s) 422, 486, 487, 2901, 3338
Bsa29I ATCGAT 1 cut(s) 280
BsaAI YACGTR 1 cut(s) 960
BsaBI GATNNNNATC 4 cut(s) 471, 756, 2526, 3897
BsaI GGTCTC 2 cut(s) 841, 1150
BsaWI WCCGGW 5 cut(s) 9, 88, 1735, 2243, 4262
BsaXI ACNNNNNCTCC 4 cut(s) 495, 525, 3316, 3346
Bsc4I CCNNNNNNNGG 7 cut(s) 521, 1789, 1864, 2000, 2732, 2998, 3603
Bse118I RCCGGY 2 cut(s) 2243, 4480
Bse1I ACTGG 9 cut(s) 368, 411, 763, 1783, 1952, 2003, 3022, 3427, 4510
Bse21I CCTNAGG 4 cut(s) 1530, 1650, 4086, 4254
Bse3DI GCAATG 3 cut(s) 418, 3349, 3927
Bse8I GATNNNNATC 4 cut(s) 471, 756, 2526, 3897
BseBI CCWGG 5 cut(s) 737, 1859, 2473, 3228, 3908
BseCI ATCGAT 1 cut(s) 280
BseJI GATNNNNATC 4 cut(s) 471, 756, 2526, 3897
BseLI CCNNNNNNNGG 7 cut(s) 521, 1789, 1864, 2000, 2732, 2998, 3603
BseMI GCAATG 3 cut(s) 418, 3349, 3927
BseNI ACTGG 9 cut(s) 368, 411, 763, 1783, 1952, 2003, 3022, 3427, 4510
BseRI GAGGAG 4 cut(s) 1313, 1486, 1904, 2715
BseSI GKGCMC 2 cut(s) 2496, 2754
BseXI GCAGC 8 cut(s) 887, 1322, 1372, 1577, 1667, 2088, 2421, 4051
BseYI CCCAGC 2 cut(s) 3580, 3940
BsgI GTGCAG 2 cut(s) 330, 894
Bsh1236I CGCG 2 cut(s) 1329, 2580
Bsh1285I CGRYCG 1 cut(s) 89
BshFI GGCC 7 cut(s) 1779, 1842, 1862, 1909, 3225, 4348, 4441
BshNI GGYRCC 3 cut(s) 511, 524, 1208
BshTI ACCGGT 1 cut(s) 2243
BshVI ATCGAT 1 cut(s) 280
BsiEI CGRYCG 1 cut(s) 89
BsiHKAI GWGCWC 9 cut(s) 420, 2394, 2496, 2510, 2754, 2825, 2840, 3334, 3934
BsiHKCI CYCGRG 1 cut(s) 485
BslFI GGGAC 3 cut(s) 268, 2974, 3074
BslI CCNNNNNNNGG 7 cut(s) 521, 1789, 1864, 2000, 2732, 2998, 3603
BsmBI CGTCTC 1 cut(s) 561
BsmFI GGGAC 3 cut(s) 268, 2974, 3074
BsmI GAATGC 2 cut(s) 2410, 3128
BsnI GGCC 7 cut(s) 1779, 1842, 1862, 1909, 3225, 4348, 4441
Bso31I GGTCTC 2 cut(s) 841, 1150
BsoBI CYCGRG 1 cut(s) 485
Bsp1720I GCTNAGC 1 cut(s) 2839
Bsp19I CCATGG 1 cut(s) 1863
BspACI CCGC 7 cut(s) 1212, 1280, 2059, 2366, 2580, 3647, 3863
BspANI GGCC 7 cut(s) 1779, 1842, 1862, 1909, 3225, 4348, 4441
BspDI ATCGAT 1 cut(s) 280
BspFNI CGCG 2 cut(s) 1329, 2580
BspMAI CTGCAG 1 cut(s) 4495
BspQI GCTCTTC 1 cut(s) 3531
BspT107I GGYRCC 3 cut(s) 511, 524, 1208
BspTI CTTAAG 1 cut(s) 664
BspTNI GGTCTC 2 cut(s) 841, 1150
BsrDI GCAATG 3 cut(s) 418, 3349, 3927
BsrFI RCCGGY 2 cut(s) 2243, 4480
BsrI ACTGG 9 cut(s) 368, 411, 763, 1783, 1952, 2003, 3022, 3427, 4510
BssAI RCCGGY 2 cut(s) 2243, 4480
BssNAI GTATAC 1 cut(s) 4193
BssT1I CCWWGG 6 cut(s) 21, 690, 1085, 1843, 1863, 3401
Bst1107I GTATAC 1 cut(s) 4193
Bst2UI CCWGG 5 cut(s) 737, 1859, 2473, 3228, 3908
BstAFI CTTAAG 1 cut(s) 664
BstAPI GCANNNNNTGC 1 cut(s) 4490
BstBAI YACGTR 1 cut(s) 960
BstC8I GCNNGC 3 cut(s) 3433, 4334, 4482
BstDSI CCRYGG 2 cut(s) 1863, 4520
BstEII GGTNACC 1 cut(s) 2485
BstFNI CGCG 2 cut(s) 1329, 2580
BstHHI GCGC 3 cut(s) 1329, 2439, 4252
BstMCI CGRYCG 1 cut(s) 89
BstNI CCWGG 5 cut(s) 737, 1859, 2473, 3228, 3908
BstNSI RCATGY 1 cut(s) 1084
BstPI GGTNACC 1 cut(s) 2485
BstSFI CTRYAG 5 cut(s) 156, 3219, 3718, 3914, 4491
BstSLI GKGCMC 2 cut(s) 2496, 2754
BstUI CGCG 2 cut(s) 1329, 2580
BstV1I GCAGC 8 cut(s) 887, 1322, 1372, 1577, 1667, 2088, 2421, 4051
BstV2I GAAGAC 2 cut(s) 1530, 2207
BstXI CCANNNNNNTGG 2 cut(s) 500, 3763
BstZ17I GTATAC 1 cut(s) 4193
Bsu15I ATCGAT 1 cut(s) 280
Bsu36I CCTNAGG 4 cut(s) 1530, 1650, 4086, 4254
BsuI GTATCC 1 cut(s) 4524
BsuRI GGCC 7 cut(s) 1779, 1842, 1862, 1909, 3225, 4348, 4441
BsuTUI ATCGAT 1 cut(s) 280
BtgI CCRYGG 2 cut(s) 1863, 4520
BtrI CACGTC 1 cut(s) 2749
BtsI GCAGTG 2 cut(s) 2040, 2494
BtsIMutI CAGTG 4 cut(s) 201, 1790, 2040, 2494
Cac8I GCNNGC 3 cut(s) 3433, 4334, 4482
CaiI CAGNNNCTG 2 cut(s) 3227, 3866
CfoI GCGC 3 cut(s) 1329, 2439, 4252
Cfr10I RCCGGY 2 cut(s) 2243, 4480
Cfr13I GGNCC 7 cut(s) 4, 725, 1840, 1907, 3061, 3224, 3452
Cfr9I CCCGGG 1 cut(s) 485
ClaI ATCGAT 1 cut(s) 280
CseI GACGC 2 cut(s) 2006, 3625
CspAI ACCGGT 1 cut(s) 2243
DraI TTTAAA 5 cut(s) 393, 1561, 1815, 2128, 2421
DraIII CACNNNGTG 1 cut(s) 521
EciI GGCGGA 2 cut(s) 1295, 3662
Ecl136II GAGCTC 4 cut(s) 2508, 2823, 3332, 3932
Eco130I CCWWGG 6 cut(s) 21, 690, 1085, 1843, 1863, 3401
Eco147I AGGCCT 1 cut(s) 1779
Eco24I GRGCYC 5 cut(s) 2284, 2510, 2825, 3334, 3934
Eco31I GGTCTC 2 cut(s) 841, 1150
Eco47I GGWCC 4 cut(s) 4, 725, 3061, 3452
Eco53kI GAGCTC 4 cut(s) 2508, 2823, 3332, 3932
Eco57I CTGAAG 5 cut(s) 927, 1335, 1565, 3512, 3596
Eco81I CCTNAGG 4 cut(s) 1530, 1650, 4086, 4254
Eco88I CYCGRG 1 cut(s) 485
Eco91I GGTNACC 1 cut(s) 2485
EcoICRI GAGCTC 4 cut(s) 2508, 2823, 3332, 3932
EcoO109I RGGNCCY 3 cut(s) 725, 1907, 3224
EcoO65I GGTNACC 1 cut(s) 2485
EcoRI GAATTC 1 cut(s) 4354
EcoRII CCWGG 5 cut(s) 735, 1857, 2471, 3226, 3906
EcoT14I CCWWGG 6 cut(s) 21, 690, 1085, 1843, 1863, 3401
EcoT38I GRGCYC 5 cut(s) 2284, 2510, 2825, 3334, 3934
ErhI CCWWGG 6 cut(s) 21, 690, 1085, 1843, 1863, 3401
Esp3I CGTCTC 1 cut(s) 561
FalI AAGNNNNNCTT 4 cut(s) 1740, 1772, 2944, 2976
FaqI GGGAC 3 cut(s) 268, 2974, 3074
FauNDI CATATG 1 cut(s) 4384
FblI GTMKAC 3 cut(s) 927, 982, 4192
FriOI GRGCYC 5 cut(s) 2284, 2510, 2825, 3334, 3934
GlaI GCGC 3 cut(s) 1328, 2438, 4251
GsaI CCCAGC 2 cut(s) 3584, 3944
GsuI CTGGAG 4 cut(s) 1692, 1766, 2801, 4263
HaeIII GGCC 7 cut(s) 1779, 1842, 1862, 1909, 3225, 4348, 4441
HgaI GACGC 2 cut(s) 2006, 3625
HhaI GCGC 3 cut(s) 1329, 2439, 4252
Hin6I GCGC 3 cut(s) 1327, 2437, 4250
HinP1I GCGC 3 cut(s) 1327, 2437, 4250
HincII GTYRAC 1 cut(s) 3534
HindII GTYRAC 1 cut(s) 3534
HindIII AAGCTT 2 cut(s) 1317, 3571
HpaI GTTAAC 1 cut(s) 3534
Hpy99I CGWCG 1 cut(s) 340
HpyCH4IV ACGT 6 cut(s) 520, 959, 2131, 2306, 2748, 3536
HpySE526I ACGT 6 cut(s) 520, 959, 2131, 2306, 2748, 3536
HspAI GCGC 3 cut(s) 1327, 2437, 4250
KpnI GGTACC 1 cut(s) 515
KroI GCCGGC 1 cut(s) 4480
KroNI GCCGGC 1 cut(s) 4482
KspAI GTTAAC 1 cut(s) 3534
LguI GCTCTTC 1 cut(s) 3531
Lsp1109I GCAGC 8 cut(s) 887, 1322, 1372, 1577, 1667, 2088, 2421, 4051
MaeII ACGT 6 cut(s) 520, 959, 2131, 2306, 2748, 3536
MlyI GAGTC 5 cut(s) 68, 349, 3083, 3463, 4268
MmeI TCCRAC 5 cut(s) 66, 2721, 2929, 3926, 4104
MroNI GCCGGC 1 cut(s) 4480
MroXI GAANNNNTTC 2 cut(s) 774, 3019
MslI CAYNNNNRTG 4 cut(s) 454, 498, 2589, 3761
MspA1I CMGCKG 6 cut(s) 878, 1313, 1771, 2101, 2632, 3863
MspCI CTTAAG 1 cut(s) 664
Mva1269I GAATGC 2 cut(s) 2410, 3128
MvaI CCWGG 5 cut(s) 737, 1859, 2473, 3228, 3908
MvnI CGCG 2 cut(s) 1329, 2580
NaeI GCCGGC 1 cut(s) 4482
NciI CCSGG 5 cut(s) 422, 486, 487, 2901, 3338
NcoI CCATGG 1 cut(s) 1863
NdeI CATATG 1 cut(s) 4384
NgoMIV GCCGGC 1 cut(s) 4480
NmeAIII GCCGAG 2 cut(s) 2465, 2491
NmuCI GTSAC 5 cut(s) 224, 710, 1403, 3266, 3958
NspI RCATGY 1 cut(s) 1084
PceI AGGCCT 1 cut(s) 1779
PciSI GCTCTTC 1 cut(s) 3531
PctI GAATGC 2 cut(s) 2410, 3128
PdiI GCCGGC 1 cut(s) 4482
PdmI GAANNNNTTC 2 cut(s) 774, 3019
PflFI GACNNNGTC 1 cut(s) 3779
PflMI CCANNNNNTGG 3 cut(s) 521, 1789, 1864
PinAI ACCGGT 1 cut(s) 2243
PleI GAGTC 5 cut(s) 67, 348, 3083, 3463, 4268
PpsI GAGTC 5 cut(s) 67, 348, 3083, 3463, 4268
Ppu21I YACGTR 1 cut(s) 960
PpuMI RGGWCCY 1 cut(s) 725
Psp124BI GAGCTC 4 cut(s) 2510, 2825, 3334, 3934
Psp1406I AACGTT 1 cut(s) 2306
Psp5II RGGWCCY 1 cut(s) 725
Psp6I CCWGG 5 cut(s) 735, 1857, 2471, 3226, 3906
PspEI GGTNACC 1 cut(s) 2485
PspFI CCCAGC 2 cut(s) 3580, 3940
PspGI CCWGG 5 cut(s) 735, 1857, 2471, 3226, 3906
PspPI GGNCC 7 cut(s) 4, 725, 1840, 1907, 3061, 3224, 3452
PspPPI RGGWCCY 1 cut(s) 725
PstI CTGCAG 1 cut(s) 4495
PstNI CAGNNNCTG 2 cut(s) 3227, 3866
PsyI GACNNNGTC 1 cut(s) 3779
PvuII CAGCTG 5 cut(s) 878, 1313, 1771, 2101, 2632
RseI CAYNNNNRTG 4 cut(s) 454, 498, 2589, 3761
SacI GAGCTC 4 cut(s) 2510, 2825, 3334, 3934
SapI GCTCTTC 1 cut(s) 3531
Sau96I GGNCC 7 cut(s) 4, 725, 1840, 1907, 3061, 3224, 3452
SchI GAGTC 5 cut(s) 68, 349, 3083, 3463, 4268
SfcI CTRYAG 5 cut(s) 156, 3219, 3718, 3914, 4491
SinI GGWCC 4 cut(s) 4, 725, 3061, 3452
SmaI CCCGGG 1 cut(s) 487
SmiMI CAYNNNNRTG 4 cut(s) 454, 498, 2589, 3761
SmlI CTYRAG 8 cut(s) 56, 664, 830, 1569, 3134, 3640, 3701, 4475
SmoI CTYRAG 8 cut(s) 56, 664, 830, 1569, 3134, 3640, 3701, 4475
SpeI ACTAGT 2 cut(s) 707, 1601
SseBI AGGCCT 1 cut(s) 1779
SsiI CCGC 7 cut(s) 1212, 1280, 2059, 2366, 2580, 3647, 3863
SspI AATATT 4 cut(s) 1900, 2620, 2882, 3697
SstI GAGCTC 4 cut(s) 2510, 2825, 3334, 3934
StuI AGGCCT 1 cut(s) 1779
StyI CCWWGG 6 cut(s) 21, 690, 1085, 1843, 1863, 3401
TaiI ACGT 6 cut(s) 523, 962, 2134, 2309, 2751, 3539
TaqI TCGA 8 cut(s) 102, 280, 338, 1342, 2224, 2786, 2918, 3243
TaqII GACCGA 1 cut(s) 3078
TatI WGTACW 4 cut(s) 309, 2681, 2691, 3827
TauI GCSGC 2 cut(s) 1214, 3866
TscAI CASTG 4 cut(s) 201, 1790, 2047, 2501
TseFI GTSAC 5 cut(s) 224, 710, 1403, 3266, 3958
TseI GCWGC 8 cut(s) 875, 1310, 1360, 1565, 1655, 2101, 2434, 4039
Tsp45I GTSAC 5 cut(s) 224, 710, 1403, 3266, 3958
TspGWI ACGGA 3 cut(s) 1406, 3814, 4137
TspMI CCCGGG 1 cut(s) 485
TspRI CASTG 4 cut(s) 201, 1790, 2047, 2501
Tth111I GACNNNGTC 1 cut(s) 3779
Van91I CCANNNNNTGG 3 cut(s) 521, 1789, 1864
Vha464I CTTAAG 1 cut(s) 664
VneI GTGCAC 2 cut(s) 2492, 2750
VpaK11BI GGWCC 4 cut(s) 4, 725, 3061, 3452
XapI RAATTY 9 cut(s) 808, 1108, 1607, 2601, 2608, 2710, 2811, 4143, 4354
XceI RCATGY 1 cut(s) 1084
XcmI CCANNNNNNNNNTGG 3 cut(s) 1861, 1870, 2369
XmaI CCCGGG 1 cut(s) 485
XmaJI CCTAGG 1 cut(s) 1843
XmiI GTMKAC 3 cut(s) 927, 982, 4192
XmnI GAANNNNTTC 2 cut(s) 774, 3019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.