Rorug03G0208500

DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005736
Physical Location & Seq
Forward (+)
18553189 .. 18554823
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0208500.1

Sequence Viewer

Length: 1635 bp
ATGTCTAGTATGCGCGTAGCTTCAGCCTCAATCGCCACATTACTCAAAGCCTGCAAAACCCAGTTCGGTCTTGAACAAATCCACGCCCAGATCATCCACAAAGGTCTAGAGCAAGACCACTTCGTCATCACCCAATTCATCTGCCTCGCCAACGCTCTCTCCTCCCTCTCTTACTCCACCGCCGTCTTCCGCCGCGTCAACAACCCCAGCAGCCATCTCTGGAACTCACTACTCAAAGGCCACTGCGAAAGATCCAGTTTTTCCGCCACCGTCTCTCTCTTCATTGGTATGAAGAGGGAGGAGGGCGTGGTGGATGAGTACACGTACCCGTCGGTTGTGAAGGCCTGTGCCGGCGAGGGAAGGGTCCGGGAGGGAAGGGCGGTTCATGGGTCGGCGGTGAGGTGTGGGGTTGATGGGAATGTGTTTGTGGGGACTAGCTTGGTTGACTTGTACGGGAAATGTAGGGAGGTTGTGTGTGCACGGAAGGTGTTTGATGGAATGTGTGAGAGAAATGTGGTGTCTTGGACTGCTATGGTTGTTGGGTATGCCGGTGTTGGGAATATGGTGGAGGCGAGTAAGGTGTTTGGTGAAATGCCGGAGAGGAATGCTGTGTCGTGGAATGTGGTTATTAGTGGGTTTGTTAGGTTGGGTGATTTGGGGAATGCGAGGAGGGTGTTTGAGGAGATGCCGGGGAAAGATGTGGTTTCTTTTACTACTATGATTGATGGATATGCAAAGTCCGGGGATATGGCTTCGGCCAGGTTTTTGTTTGAGCAGGCTCCGGTTAAGGATATTGTTATGTGGTCGGCGTTGATATCGGGGTATGCACAAAATGGTTTTCCCAATCAGGCTGTGAAAATATTTCTTGAGATGAGTAGTAGGAATGTGGAACCTGATGAGTTTATAATGGTGAGCTTGATGTCAGCTTGTTCCCAAGTGGGTTGCTTGGAATTGGCTGAATGGCTGGATTCGTACTTGAGCAATAGCTCAATCGATATTCGACAGCCTCATGTTCTCACGGCTCTGATCGACATGAATGCAAAGTGTGGGAATATGGAAAGAGCGACGAGTTTGTTTCAAAACATGCCAAAACGGGACCTAATTTCGTATTGTTCAATGATACAAGGGTTGTCTATTCACGGCCGAGGGAATCAAGCTGTTGCCCTTTTCAATAGGATGTTAAGTGAAGGTTTGGCTCCAGATGAGGTAGCTTTTACAGTGATCCTGACAGCTTGTAGCCGTTCCGAGCTTGTTGAGGAGGGTCGGCATTTCTTTGAATTAATGAGACATAAATACAGTATGGTTCCCACTCCTGATCACTATGCATGCATGGTTGATCTTCTTAGCCGGTCAGGATGGTTAGAAGAAGCTTATGAGCTTCTACAATCAATGCCTGTGGAACCTCATGCTGGTGCTTGGGGTGCACTACTTGGTGCATGTAAGCTACATTGCAATGTTGAGTTTGGGGAGTTGGTTGCTAATCGACTCTTTGTGCTTGAACCAAAAAGTGCTGGTAGTTATGTGCTCCTCTCCGACATCTATGCTGCATTCCACCGCTGGTTGGATGTTTCTGTTGTGAGGAACAAAATGCAGGAGAGAGGCCTTAAAAAGATACCTGGTATCAGTTCAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBINO Helicase_C SNF2_N

Protein Analysis

544

Amino Acids

60.01

Weight (kDa)

6.41

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 69 - 117 3e-06 PPR repeat family
PPR PF01535 204 - 233 4.5e-06 PPR repeat
PPR_2 PF13041 263 - 310 5.7e-09 PPR repeat family
PPR PF01535 266 - 296 3.4e-07 PPR repeat
PPR_2 PF13041 366 - 413 9.8e-10 PPR repeat family
E_motif PF20431 483 - 544 5.7e-19 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
AasI GACNNNNNNGTC 1 cut(s) 122
AccII CGCG 2 cut(s) 15, 195
AciI CCGC 7 cut(s) 180, 190, 193, 264, 380, 395, 1555
AclWI GGATC 2 cut(s) 246, 1216
AcoI YGGCCR 2 cut(s) 756, 1141
AcuI CTGAAG 1 cut(s) 6
AfaI GTAC 4 cut(s) 320, 326, 452, 974
AfiI CCNNNNNNNGG 3 cut(s) 555, 1409, 1561
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 7 cut(s) 74, 1079, 1116, 1171, 1277, 1499, 1629
AjnI CCWGG 2 cut(s) 758, 1615
AloI GAACNNNNNNTCC 2 cut(s) 366, 398
Alw21I GWGCWC 3 cut(s) 481, 1426, 1527
Alw26I GTCTC 2 cut(s) 277, 1279
Alw44I GTGCAC 2 cut(s) 477, 1422
AlwI GGATC 2 cut(s) 246, 1216
AoxI GGCC 5 cut(s) 238, 342, 756, 1141, 1600
ApaLI GTGCAC 2 cut(s) 477, 1422
ApeKI GCWGC 2 cut(s) 210, 1544
AseI ATTAAT 1 cut(s) 1280
AspLEI GCGC 1 cut(s) 15
AspS9I GGNCC 2 cut(s) 364, 1096
AsuC2I CCSGG 3 cut(s) 368, 690, 742
AsuHPI GGTGA 5 cut(s) 121, 409, 599, 662, 922
AvaII GGWCC 2 cut(s) 364, 1096
BaeGI GKGCMC 2 cut(s) 481, 1426
BaeI ACNNNNGTAYC 2 cut(s) 1112, 1145
BbsI GAAGAC 1 cut(s) 178
Bbv12I GWGCWC 3 cut(s) 481, 1426, 1527
BbvI GCAGC 2 cut(s) 222, 1531
BccI CCATC 5 cut(s) 222, 407, 488, 719, 1350
BceAI ACGGC 4 cut(s) 167, 1035, 1156, 1224
BcgI CGANNNNNNTGC 4 cut(s) 1019, 1053, 1523, 1557
BciT130I CCWGG 2 cut(s) 760, 1617
BclI TGATCA 1 cut(s) 1315
BcnI CCSGG 3 cut(s) 368, 690, 742
BcoDI GTCTC 2 cut(s) 277, 1279
BfaI CTAG 3 cut(s) 6, 107, 435
BisI GCNGC 3 cut(s) 193, 211, 1545
BlsI GCNGC 3 cut(s) 194, 212, 1546
Bme1390I CCNGG 5 cut(s) 368, 690, 742, 760, 1617
Bme18I GGWCC 2 cut(s) 364, 1096
BmgT120I GGNCC 2 cut(s) 364, 1096
BmiI GGNNCC 7 cut(s) 365, 780, 891, 1097, 1197, 1305, 1401
BmrFI CCNGG 5 cut(s) 368, 690, 742, 760, 1617
BmrI ACTGGG 1 cut(s) 55
BmsI GCATC 1 cut(s) 675
BmuI ACTGGG 1 cut(s) 55
BpiI GAAGAC 1 cut(s) 178
BpmI CTGGAG 1 cut(s) 1182
BpuEI CTTGAG 2 cut(s) 887, 997
BpuMI CCSGG 3 cut(s) 368, 690, 742
Bsa29I ATCGAT 1 cut(s) 993
BsaAI YACGTR 1 cut(s) 324
BsaJI CCNNGG 3 cut(s) 689, 741, 1144
BsaWI WCCGGW 1 cut(s) 781
BsaXI ACNNNNNCTCC 4 cut(s) 143, 173, 458, 488
Bsc4I CCNNNNNNNGG 3 cut(s) 555, 1409, 1561
Bse118I RCCGGY 3 cut(s) 350, 548, 1347
Bse1I ACTGG 2 cut(s) 61, 255
Bse3DI GCAATG 2 cut(s) 1447, 1459
BseBI CCWGG 2 cut(s) 760, 1617
BseCI ATCGAT 1 cut(s) 993
BseDI CCNNGG 3 cut(s) 689, 741, 1144
BseGI GGATG 5 cut(s) 93, 319, 1182, 1361, 1570
BseLI CCNNNNNNNGG 3 cut(s) 555, 1409, 1561
BseMI GCAATG 2 cut(s) 1447, 1459
BseNI ACTGG 2 cut(s) 61, 255
BseRI GAGGAG 6 cut(s) 151, 314, 682, 695, 1271, 1517
BseSI GKGCMC 2 cut(s) 481, 1426
BseX3I CGGCCG 1 cut(s) 1141
BseXI GCAGC 2 cut(s) 222, 1531
BseYI CCCAGC 1 cut(s) 206
Bsh1236I CGCG 2 cut(s) 15, 195
Bsh1285I CGRYCG 1 cut(s) 1144
BshFI GGCC 5 cut(s) 240, 344, 758, 1143, 1602
BshVI ATCGAT 1 cut(s) 993
BsiEI CGRYCG 1 cut(s) 1144
BsiHKAI GWGCWC 3 cut(s) 481, 1426, 1527
BsiSI CCGG 8 cut(s) 351, 367, 549, 596, 689, 741, 782, 1348
BslFI GGGAC 2 cut(s) 445, 1109
BslI CCNNNNNNNGG 3 cut(s) 555, 1409, 1561
BsmAI GTCTC 2 cut(s) 277, 1279
BsmBI CGTCTC 1 cut(s) 277
BsmFI GGGAC 2 cut(s) 445, 1109
BsmI GAATGC 4 cut(s) 610, 667, 1042, 1547
BsnI GGCC 5 cut(s) 240, 344, 758, 1143, 1602
Bsp1286I GDGCHC 3 cut(s) 481, 1426, 1527
Bsp143I GATC 6 cut(s) 90, 251, 1026, 1221, 1315, 1336
BspACI CCGC 7 cut(s) 180, 190, 193, 264, 380, 395, 1555
BspANI GGCC 5 cut(s) 240, 344, 758, 1143, 1602
BspDI ATCGAT 1 cut(s) 993
BspFNI CGCG 2 cut(s) 15, 195
BspLI GGNNCC 7 cut(s) 365, 780, 891, 1097, 1197, 1305, 1401
BspPI GGATC 2 cut(s) 246, 1216
BsrDI GCAATG 2 cut(s) 1447, 1459
BsrFI RCCGGY 3 cut(s) 350, 548, 1347
BsrI ACTGG 2 cut(s) 61, 255
BssAI RCCGGY 3 cut(s) 350, 548, 1347
BssECI CCNNGG 3 cut(s) 689, 741, 1144
BssMI GATC 6 cut(s) 90, 251, 1026, 1221, 1315, 1336
Bst2UI CCWGG 2 cut(s) 760, 1617
Bst4CI ACNGT 3 cut(s) 271, 1219, 1298
Bst6I CTCTTC 2 cut(s) 284, 287
BstBAI YACGTR 1 cut(s) 324
BstC8I GCNNGC 4 cut(s) 52, 352, 777, 1327
BstDEI CTNAG 1 cut(s) 1343
BstF5I GGATG 5 cut(s) 93, 319, 1182, 1361, 1570
BstFNI CGCG 2 cut(s) 15, 195
BstHHI GCGC 1 cut(s) 15
BstKTI GATC 6 cut(s) 93, 254, 1029, 1224, 1318, 1339
BstMAI GTCTC 2 cut(s) 277, 1279
BstMBI GATC 6 cut(s) 90, 251, 1026, 1221, 1315, 1336
BstMCI CGRYCG 1 cut(s) 1144
BstMWI GCNNNNNNNGC 2 cut(s) 32, 1421
BstNI CCWGG 2 cut(s) 760, 1617
BstNSI RCATGY 3 cut(s) 1087, 1329, 1440
BstSCI CCNGG 5 cut(s) 366, 688, 740, 758, 1615
BstSLI GKGCMC 2 cut(s) 481, 1426
BstUI CGCG 2 cut(s) 15, 195
BstV1I GCAGC 2 cut(s) 222, 1531
BstV2I GAAGAC 1 cut(s) 178
BstX2I RGATCY 1 cut(s) 251
BstYI RGATCY 1 cut(s) 251
BstZI CGGCCG 1 cut(s) 1141
Bsu15I ATCGAT 1 cut(s) 993
BsuRI GGCC 5 cut(s) 240, 344, 758, 1143, 1602
BsuTUI ATCGAT 1 cut(s) 993
BtsCI GGATG 5 cut(s) 93, 319, 1182, 1361, 1570
BtsI GCAGTG 1 cut(s) 241
BtsIMutI CAGTG 2 cut(s) 241, 1224
Cac8I GCNNGC 4 cut(s) 52, 352, 777, 1327
CfoI GCGC 1 cut(s) 15
Cfr10I RCCGGY 3 cut(s) 350, 548, 1347
Cfr13I GGNCC 2 cut(s) 364, 1096
ClaI ATCGAT 1 cut(s) 993
CseI GACGC 1 cut(s) 184
CsiI ACCWGGT 1 cut(s) 1615
Csp6I GTAC 4 cut(s) 319, 325, 451, 973
CspCI CAANNNNNGTGG 2 cut(s) 1377, 1412
CviAII CATG 8 cut(s) 386, 1010, 1033, 1084, 1326, 1330, 1406, 1437
CviQI GTAC 4 cut(s) 319, 325, 451, 973
DdeI CTNAG 1 cut(s) 1343
DpnI GATC 6 cut(s) 92, 253, 1028, 1223, 1317, 1338
DpnII GATC 6 cut(s) 90, 251, 1026, 1221, 1315, 1336
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 2 cut(s) 756, 1141
EagI CGGCCG 1 cut(s) 1141
Eam1104I CTCTTC 2 cut(s) 284, 287
EarI CTCTTC 2 cut(s) 284, 287
EciI GGCGGA 2 cut(s) 179, 253
EclXI CGGCCG 1 cut(s) 1141
Eco147I AGGCCT 2 cut(s) 344, 1602
Eco32I GATATC 1 cut(s) 816
Eco47I GGWCC 2 cut(s) 364, 1096
Eco52I CGGCCG 1 cut(s) 1141
Eco57I CTGAAG 1 cut(s) 6
EcoO109I RGGNCCY 1 cut(s) 1096
EcoRII CCWGG 2 cut(s) 758, 1615
EcoRV GATATC 1 cut(s) 816
EcoT22I ATGCAT 2 cut(s) 1327, 1331
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 8 cut(s) 389, 1013, 1036, 1087, 1329, 1333, 1409, 1440
FaqI GGGAC 2 cut(s) 445, 1109
FatI CATG 8 cut(s) 385, 1009, 1032, 1083, 1325, 1329, 1405, 1436
FbaI TGATCA 1 cut(s) 1315
Fnu4HI GCNGC 3 cut(s) 193, 211, 1545
FokI GGATG 5 cut(s) 80, 326, 1189, 1368, 1577
Fsp4HI GCNGC 3 cut(s) 193, 211, 1545
FspBI CTAG 3 cut(s) 6, 107, 435
GlaI GCGC 1 cut(s) 14
GluI GCNGC 3 cut(s) 193, 211, 1545
GsaI CCCAGC 1 cut(s) 210
GsuI CTGGAG 1 cut(s) 1182
HaeIII GGCC 5 cut(s) 240, 344, 758, 1143, 1602
HapII CCGG 8 cut(s) 351, 367, 549, 596, 689, 741, 782, 1348
HgaI GACGC 1 cut(s) 184
HhaI GCGC 1 cut(s) 15
Hin1II CATG 8 cut(s) 389, 1013, 1036, 1087, 1329, 1333, 1409, 1440
Hin6I GCGC 1 cut(s) 13
HinP1I GCGC 1 cut(s) 13
HincII GTYRAC 2 cut(s) 199, 445
HindII GTYRAC 2 cut(s) 199, 445
HindIII AAGCTT 1 cut(s) 1368
HinfI GANTC 3 cut(s) 968, 1150, 1485
HpaII CCGG 8 cut(s) 351, 367, 549, 596, 689, 741, 782, 1348
HphI GGTGA 5 cut(s) 121, 409, 599, 662, 922
Hpy166II GTNNAC 5 cut(s) 199, 321, 445, 479, 1424
Hpy188I TCNGA 3 cut(s) 1026, 1246, 1534
Hpy188III TCNNGA 8 cut(s) 71, 107, 220, 866, 1199, 1225, 1313, 1353
Hpy8I GTNNAC 5 cut(s) 199, 321, 445, 479, 1424
Hpy99I CGWCG 2 cut(s) 334, 1069
HpyAV CCTTC 5 cut(s) 334, 354, 369, 478, 1181
HpyCH4III ACNGT 3 cut(s) 271, 1219, 1298
HpyCH4IV ACGT 1 cut(s) 323
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 1421
HpyF3I CTNAG 1 cut(s) 1343
HpySE526I ACGT 1 cut(s) 323
Hsp92II CATG 8 cut(s) 389, 1013, 1036, 1087, 1329, 1333, 1409, 1440
HspAI GCGC 1 cut(s) 13
KroI GCCGGC 1 cut(s) 350
KroNI GCCGGC 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 1315
Kzo9I GATC 6 cut(s) 90, 251, 1026, 1221, 1315, 1336
LmnI GCTCC 3 cut(s) 784, 1201, 1530
Lsp1109I GCAGC 2 cut(s) 222, 1531
LweI GCATC 1 cut(s) 675
MabI ACCWGGT 1 cut(s) 1615
MaeI CTAG 3 cut(s) 6, 107, 435
MaeII ACGT 1 cut(s) 323
MalI GATC 6 cut(s) 92, 253, 1028, 1223, 1317, 1338
MboI GATC 6 cut(s) 90, 251, 1026, 1221, 1315, 1336
MboII GAAGA 5 cut(s) 178, 271, 304, 1331, 1376
MflI RGATCY 1 cut(s) 251
MhlI GDGCHC 3 cut(s) 481, 1426, 1527
MluCI AATT 5 cut(s) 134, 950, 1101, 1277, 1629
MlyI GAGTC 1 cut(s) 1479
MmeI TCCRAC 2 cut(s) 1542, 1557
Mph1103I ATGCAT 2 cut(s) 1327, 1331
MroNI GCCGGC 1 cut(s) 350
MseI TTAA 4 cut(s) 786, 1181, 1280, 1605
MslI CAYNNNNRTG 3 cut(s) 287, 1410, 1452
MspA1I CMGCKG 1 cut(s) 1557
MspI CCGG 8 cut(s) 351, 367, 549, 596, 689, 741, 782, 1348
MspR9I CCNGG 5 cut(s) 368, 690, 742, 760, 1617
Mva1269I GAATGC 4 cut(s) 610, 667, 1042, 1547
MvaI CCWGG 2 cut(s) 760, 1617
MvnI CGCG 2 cut(s) 15, 195
MwoI GCNNNNNNNGC 2 cut(s) 32, 1421
NaeI GCCGGC 1 cut(s) 352
NciI CCSGG 3 cut(s) 368, 690, 742
NdeII GATC 6 cut(s) 90, 251, 1026, 1221, 1315, 1336
NgoMIV GCCGGC 1 cut(s) 350
NlaIII CATG 8 cut(s) 389, 1013, 1036, 1087, 1329, 1333, 1409, 1440
NlaIV GGNNCC 7 cut(s) 365, 780, 891, 1097, 1197, 1305, 1401
NmeAIII GCCGAG 1 cut(s) 1169
NsiI ATGCAT 2 cut(s) 1327, 1331
NspI RCATGY 3 cut(s) 1087, 1329, 1440
PaeI GCATGC 1 cut(s) 1329
PceI AGGCCT 2 cut(s) 344, 1602
PctI GAATGC 4 cut(s) 610, 667, 1042, 1547
PdiI GCCGGC 1 cut(s) 352
PfeI GAWTC 2 cut(s) 968, 1150
PfoI TCCNGGA 1 cut(s) 366
PkrI GCNGC 3 cut(s) 194, 212, 1546
PleI GAGTC 1 cut(s) 1479
PpsI GAGTC 1 cut(s) 1479
Ppu21I YACGTR 1 cut(s) 324
PpuMI RGGWCCY 1 cut(s) 1096
PshBI ATTAAT 1 cut(s) 1280
PsiI TTATAA 1 cut(s) 905
Psp5II RGGWCCY 1 cut(s) 1096
Psp6I CCWGG 2 cut(s) 758, 1615
PspFI CCCAGC 1 cut(s) 206
PspGI CCWGG 2 cut(s) 758, 1615
PspN4I GGNNCC 7 cut(s) 365, 780, 891, 1097, 1197, 1305, 1401
PspPI GGNCC 2 cut(s) 364, 1096
PspPPI RGGWCCY 1 cut(s) 1096
PsuI RGATCY 1 cut(s) 251
RsaI GTAC 4 cut(s) 320, 326, 452, 974
RsaNI GTAC 4 cut(s) 319, 325, 451, 973
RseI CAYNNNNRTG 3 cut(s) 287, 1410, 1452
SaqAI TTAA 4 cut(s) 786, 1181, 1280, 1605
SatI GCNGC 3 cut(s) 193, 211, 1545
Sau3AI GATC 6 cut(s) 90, 251, 1026, 1221, 1315, 1336
Sau96I GGNCC 2 cut(s) 364, 1096
SchI GAGTC 1 cut(s) 1479
ScrFI CCNGG 5 cut(s) 368, 690, 742, 760, 1617
SduI GDGCHC 3 cut(s) 481, 1426, 1527
SexAI ACCWGGT 1 cut(s) 1615
SfaNI GCATC 1 cut(s) 675
SinI GGWCC 2 cut(s) 364, 1096
SmiMI CAYNNNNRTG 3 cut(s) 287, 1410, 1452
SmlI CTYRAG 2 cut(s) 866, 976
SmoI CTYRAG 2 cut(s) 866, 976
SphI GCATGC 1 cut(s) 1329
Sse9I AATT 5 cut(s) 134, 950, 1101, 1277, 1629
SseBI AGGCCT 2 cut(s) 344, 1602
SsiI CCGC 7 cut(s) 180, 190, 193, 264, 380, 395, 1555
SspI AATATT 1 cut(s) 861
SspMI CTAG 3 cut(s) 6, 107, 435
StuI AGGCCT 2 cut(s) 344, 1602
StyD4I CCNGG 5 cut(s) 366, 688, 740, 758, 1615
TaaI ACNGT 3 cut(s) 271, 1219, 1298
TaiI ACGT 1 cut(s) 326
TaqI TCGA 4 cut(s) 993, 1000, 1029, 1483
TaqII GACCGA 1 cut(s) 56
TasI AATT 5 cut(s) 134, 950, 1101, 1277, 1629
TatI WGTACW 1 cut(s) 318
TauI GCSGC 1 cut(s) 195
TfiI GAWTC 2 cut(s) 968, 1150
Tru1I TTAA 4 cut(s) 786, 1181, 1280, 1605
Tru9I TTAA 4 cut(s) 786, 1181, 1280, 1605
TscAI CASTG 2 cut(s) 248, 1224
TseI GCWGC 2 cut(s) 210, 1544
TspDTI ATGAA 5 cut(s) 127, 271, 305, 374, 1049
TspGWI ACGGA 1 cut(s) 496
TspRI CASTG 2 cut(s) 248, 1224
VneI GTGCAC 2 cut(s) 477, 1422
VpaK11BI GGWCC 2 cut(s) 364, 1096
VspI ATTAAT 1 cut(s) 1280
XbaI TCTAGA 1 cut(s) 106
XceI RCATGY 3 cut(s) 1087, 1329, 1440
XspI CTAG 3 cut(s) 6, 107, 435
Zsp2I ATGCAT 2 cut(s) 1327, 1331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.