Rh3BG293500

DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0005736
Physical Location & Seq
Forward (+)
29440904 .. 29452153
11250 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG293500.1

Sequence Viewer

Length: 4023 bp
ATGGACCACAGGCGCCAATCCAAGGACTCTCTCTCCTCCTCCTACTCCAACCTCTTCAATCTTGAGCCTTTGATGAACTTTCAGCTTCCACAGCCAGATGATGATTTTGATTATTACGGCAATAGTAGTCAGGATGAGAGCAGAGGTAGCCAAGGTGGAGCTGCTGGTAATGGGATGATGTCCGACCGCGAACTGAATTCAGTAAAGAAAAGGAGGCGGTCTCAAAACAGTGATTACGAGGAGGATGACAGTTATTACGGGACGCACATTACGGAGGACAAGTATAGATCAATGCTTGGAGAACACATTCAGAAGTACAAAAGGAGGTTCAAGGATTCCCCGTCAAGTCCTGCACCAATCCAGATGGGGATTCCGGTTTCAAAGGGTAATAAGGGTTCGAAATCGAGGAAACTGGCGAATGAGAACCGAGGAGGGTTTTATGAAATGGAAACCACGTCTGAGTGGCTCAATGATACTATTCCTCAGAAACCAGGGAACTTCCATGATGCTGATTTTGCGCCACAAAGTGCCGCTGATAGAGTAATATATGAACCTCCTTATCTGGATATTGGAGATGGTTTCACATACAAGATTCCACCAATTTATGATAAACTGGTGACATCTTTACACTTGCCGAGCTTTTCTGATTTTCGGGTGGAGGAAGTTTACTTAAAAGGTACATTGGATTTGGGATCCTTAGCAGAAATGATGGGTAGCGATAAAAGGTTTGGTCCAAAAAATCGGGCAGGAATGGGGGAGCCCTATCCCCAGTATGAGTCCCTTCAGGCAAGATTGAAGGCCTTGTCCACTTCTAGCTCTGCTCAAAAGTTCAGCCTCAAAGTTTCTGATATTGGGTTGAATTCCTCCATTCCAGAGGGGGCAGCTGGAAGTATAAAGAGGTCTATTTTGTCCGAGGGTGGTGTCTTGCAAGTCTACTATGTGAAGGTTTTGGAGAAAGGAGACACATATGAGATAATTGAACGAAGCCTACCCAAGAAGCAAAAGGTACAGAAAGATCCTTCTCAGATTGAGAAGGAGGAAATGGAAAGGATTGGAAGAGTTTGGGTAAACATTGTAAGAAGAGACATACCAAAGCATCATAGAATGTTTACTACTTTTCACCGCAAGCAACTAATTGATGCCAAGAGGGTTTCAGAGAACTGTCAAAGAGAGGTAAAAATGAAGGTGAGTAGATCACTTAAAGTGATGAGGGGTGCTGCAATTCGCACAAGGAAACTAGCCAGAGACATGCTACTGCTTTGGAAGAGAATAGACAAAGAGATGGCAGAAGTGAGGAAGAAAGAGGAAAAAGAAGCTATTGAAATGCGCAAGCGTCTGGAGGAGGAAAGAGAAGCAAAGAGACAGGAGCAAAGGCTCAATTTTCTCATAAAACAAACCGAACTTTACAGTCACTTCATGCAGAACAAGTTAAGTTCACAGCCATCTGAAGACTTGCCTGTAGGAGACGAAAACCAAGATGACCAAGACGTATCTCCAAGCTCCTCAGATATTGAGAATATTGAAGAAGATTCTGAGGAAGCTGAACTGAAGAAGGAGGCATTAAAAGCTGCTCAAGATGCAGTTTCAAAGCAGAAAAAGTTAACTAGCGCATTTGATGACGAGTGTCTGAAACTGCGTGAAGCCTCTGAACCTGAGGCACCACAGGATGTTGCTGGAGCTAATAACATTGATCTGCATAACCCTTCCACTATGCCTGTTACGTCAACCGTTCAGACACCAGAGTTGTTCAAAGGTTCCCTTAAAGAATACCAGCTAAAAGGCCTCCAGTGGCTGGTTAATTGTTATGAGCAGGGTTTAAATGGCATCCTTGCTGATGAGATGGGCCTGGGTAAGACCATCCAGGCCATGGCATTTTTGGCTCATTTGGCTGAGGAGAAAAATATATGGGGGCCTTTTCTGGTTGTTGCTCCTGCTTCTGTCTTGAACAACTGGGCTGATGAAATTAGTCGTTTCTGCCCCGACTTGAAAACTCTTCCGTATTGGGGTGGCCTTCAAGAGCGTACGATACTTAGGAAGAAGATCAACGCTAAGAAACTTTACCGTAGGGATGCTGGGTTTCACATTCTCATTACCAGCTACCAGTTGCTAGTGGCTGATGAGAAGTCTTTCCGGCGTGTGAAATGGCAATATATGGTATTGGATGAAGCACAAGCTATCAAAAGTTCTAACAGTATAAGATGGAAGACACTGCTCAGCTTTAATTGTCGAAATCGTTTGCTGCTTACTGGTACACCTATCCAGAATAATATGGCAGAGTTGTGGGCCCTACTACATTTCATTATGCCAACCTTATTTGACAGTCATGAACAATTCAATGAGTGGTTCTCTAAAGGAATTGAAAATCATGCGGAACATGGGGGTACTTTGAACGAGCACCAGCTTAACAGATTGCATTCGATATTGAAGCCATTCATGCTCCGTCGAGTTAAGACAGATGTGATTTCTGAGCTGACCAGGAAAACTGAAGTTACTGTGCACTGCAAGTTGAGTTCTCGGCAACAAGCTTTTTATCAAGCTATCAAAAACAAGATATCTCTTGCTGAGCTGTTTGACAACAATCGTGGGCATCTTAATGAGAAGAAAATTTTGAACTTAATGAATATTGTCATTCAGCTAAGAAAAGTCTGCAACCATCCTGAGTTATTTGAGAGGAATGAAGGAAGCACATATCTTCACTTTGGTGAGATTTCAAATTCTCTTCTTCCTCCGCCATTTGGGGAGTTGGAGGACGTACACTACTCGGGAGGTCAAAATCCTATAACATACAAGGTCCCCAAACTTTTATATCAAGAAATACTCCAGAATTCTGAAACGTTTTGCTCTGCAGTTCGGCATGGTGTCTACAGTGAATCTTTTCAGAAACATTTTAACATATTTTCACCGCAAAATGTGTATCGATCAATATTCTTGCAAGAGAACGACTCAGATGAGTTGTCGGTTGGAAGTGGAACGTTTGGTTTTACTCATCTGATGGATCTGTCCCCTGCAGAGGTTGCATTTTTAGGAACTGGTTCTTTTATGGAGCGGCTAATGTTTTCCATTATGAGATGGGACCGGCAGTTTTTGGATGGATTGATAGACACACTAATGGAAACCATGGATGATGATTCTGAATGCAGCTTTCTTGAGAGTGGAAAAGTGAGAGCTGTTACACGTATGTTGCTGATGCCTTCAAGATCTATAACTACCATCTTTCAGAAGAAATTGGCCTCTGGACCTGGTGGTACTCCATTTGAGGGTCTAGTTGTCTCTCATCGGGACAGGCTTCTCTCTAATATTAGGCTTCTCCGTTCGACATACACATTCATCCCCCGAACTAGAGCTCCCCCAGTTAATGCCCATTGCTCAGATCGAAACTTCACTTACAAAATGAGTGAAGAACAACATTACCCCTGGGTGAAGAGGTTGTTTTCTGGCTTTGCGCGAACATCTGACTACAATGGACCCAGAAAACCTGAGACTCCTCATCATCTAATACAAGAGATTGATTCTGAACTGCCTGTTGCACATTCAGCTCTTCAGTTAACATACAGAATTTTTGGGTCTTGTCCTCCAATGCAAAGCTTCGACCCGTCAAAAATGCTCACTGATTCGGGGAAACTTCAGACACTTGATATATTGTTGAAACGCTTGAGGGCAGAAAATCATCGTGTTCTCTTGTTTGCACAGATGACAAAGATGCTGAATATTCTTGAGGACTACATGAACTACAGGAAATATAGATACCTCAGACTCGATGGATCTTCCACCATTATGGATCGTAGAGACATGGTCAGAGACTTCCAGCAACGAAGTGATATTTTTGTTTTCTTGCTGAGTACAAGAGCTGGTGGACTAGGCATTAACTTGACAGCTGCAGATACTGTCATATTTTATGAAAGTGATTGGAATCCGACCCTGGATCTACAGGCAATGGATAGAGCTCATCGGTTGGGCCAGACAAAAGATGTGAGTTTCTTTTTGCTAATCCAGCGTACACCAATCCGCTCCCAAATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBINO Helicase_C SNF2_N

Protein Analysis

1340

Amino Acids

153.85

Weight (kDa)

8.28

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBINO PF13892 349 - 479 9e-45 DNA-binding domain
ResIII PF04851 586 - 753 1.3e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 590 - 888 1.6e-85 SNF2-related domain
Helicase_C PF00271 1209 - 1319 2.3e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1328
AccB1I GGYRCC 2 cut(s) 12, 1657
AccB7I CCANNNNNTGG 2 cut(s) 1792, 1867
AccBSI CCGCTC 2 cut(s) 3046, 4008
AccI GTMKAC 2 cut(s) 933, 2862
AccII CGCG 2 cut(s) 189, 3445
AciI CCGC 9 cut(s) 187, 217, 531, 1123, 2369, 2729, 2903, 3046, 4006
AclI AACGTT 2 cut(s) 2834, 2972
AclWI GGATC 7 cut(s) 687, 700, 1010, 3003, 3769, 3786, 3930
AcsI RAATTY 6 cut(s) 196, 859, 2604, 2713, 2824, 3555
AcuI CTGAAG 6 cut(s) 767, 1467, 1568, 2505, 3524, 3608
AcyI GRCGYC 1 cut(s) 13
AdeI CACNNNGTG 1 cut(s) 527
AfiI CCNNNNNNNGG 7 cut(s) 22, 1792, 1867, 2003, 2735, 3010, 3257
AflIII ACRYGT 1 cut(s) 3173
AjiI CACGTC 1 cut(s) 456
AjnI CCWGG 7 cut(s) 490, 1845, 1860, 2474, 3238, 3413, 3918
AleI CACNNNNGTG 1 cut(s) 2700
AloI GAACNNNNNNTCC 2 cut(s) 3328, 3360
Alw21I GWGCWC 4 cut(s) 2397, 2499, 3346, 3946
Alw44I GTGCAC 1 cut(s) 2495
AlwI GGATC 7 cut(s) 687, 700, 1010, 3003, 3769, 3786, 3930
AlwNI CAGNNNCTG 2 cut(s) 1792, 3884
Ama87I CYCGRG 1 cut(s) 2761
AoxI GGCC 9 cut(s) 798, 1780, 1843, 1863, 1910, 2008, 2283, 3228, 3955
ApaI GGGCCC 1 cut(s) 2287
ApaLI GTGCAC 1 cut(s) 2495
ApeKI GCWGC 7 cut(s) 161, 881, 1217, 1568, 2239, 3138, 3875
ApoI RAATTY 6 cut(s) 196, 859, 2604, 2713, 2824, 3555
Asp700I GAANNNNTTC 5 cut(s) 306, 2126, 2429, 2874, 3031
AspLEI GCGC 5 cut(s) 15, 520, 1329, 1610, 3445
AsuHPI GGTGA 6 cut(s) 628, 1112, 1198, 2714, 2892, 3430
AsuII TTCGAA 1 cut(s) 398
AvaI CYCGRG 1 cut(s) 2761
AvaII GGWCC 6 cut(s) 4, 731, 2791, 3073, 3236, 3464
AxyI CCTNAGG 1 cut(s) 1653
BaeGI GKGCMC 2 cut(s) 2287, 2499
BamHI GGATCC 1 cut(s) 692
BanI GGYRCC 2 cut(s) 12, 1657
BanII GRGCYC 4 cut(s) 762, 2287, 3346, 3946
BbsI GAAGAC 2 cut(s) 1455, 2210
Bbv12I GWGCWC 4 cut(s) 2397, 2499, 3346, 3946
BbvCI CCTCAGC 1 cut(s) 1890
BbvI GCAGC 7 cut(s) 148, 893, 1204, 1555, 2226, 3150, 3862
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 7 cut(s) 492, 1847, 1862, 2476, 3240, 3415, 3920
BfaI CTAG 7 cut(s) 813, 1238, 1605, 2108, 3263, 3339, 3857
BfmI CTRYAG 7 cut(s) 1458, 2844, 2863, 3006, 3730, 3876, 3926
BfoI RGCGCY 1 cut(s) 16
BglII AGATCT 1 cut(s) 3197
BisI GCNGC 9 cut(s) 162, 531, 882, 1218, 1569, 2240, 3047, 3139, 3876
BlpI GCTNAGC 2 cut(s) 2213, 2562
BlsI GCNGC 9 cut(s) 163, 532, 883, 1219, 1570, 2241, 3048, 3140, 3877
Bme1390I CCNGG 7 cut(s) 492, 1847, 1862, 2476, 3240, 3415, 3920
Bme18I GGWCC 6 cut(s) 4, 731, 2791, 3073, 3236, 3464
BmeT110I CYCGRG 1 cut(s) 2761
BmgBI CACGTC 1 cut(s) 456
BmrFI CCNGG 7 cut(s) 492, 1847, 1862, 2476, 3240, 3415, 3920
BmrI ACTGGG 3 cut(s) 763, 1960, 3344
BmsI GCATC 9 cut(s) 496, 1105, 1129, 1567, 1833, 2059, 2596, 3177, 3690
BmuI ACTGGG 3 cut(s) 763, 1960, 3344
BoxI GACNNNNGTC 1 cut(s) 1623
BpiI GAAGAC 2 cut(s) 1455, 2210
BplI GAGNNNNNCTC 6 cut(s) 205, 237, 2330, 2362, 2927, 2959
BpmI CTGGAG 4 cut(s) 1358, 1695, 1769, 2804
Bpu10I CCTNAGC 2 cut(s) 697, 1890
Bpu1102I GCTNAGC 2 cut(s) 2213, 2562
Bpu14I TTCGAA 1 cut(s) 398
BpuEI CTTGAG 5 cut(s) 83, 1557, 3167, 3673, 3734
Bsa29I ATCGAT 1 cut(s) 2917
BsaAI YACGTR 1 cut(s) 3176
BsaBI GATNNNNATC 1 cut(s) 3909
BsaHI GRCGYC 1 cut(s) 13
BsaI GGTCTC 1 cut(s) 225
BsaWI WCCGGW 1 cut(s) 373
BsaXI ACNNNNNCTCC 6 cut(s) 17, 47, 266, 296, 3328, 3358
Bsc4I CCNNNNNNNGG 7 cut(s) 22, 1792, 1867, 2003, 2735, 3010, 3257
Bse118I RCCGGY 1 cut(s) 3075
Bse1I ACTGG 9 cut(s) 417, 618, 769, 1786, 1955, 2101, 2251, 3034, 3350
Bse21I CCTNAGG 1 cut(s) 1653
Bse3DI GCAATG 2 cut(s) 3361, 3939
Bse8I GATNNNNATC 1 cut(s) 3909
BseBI CCWGG 7 cut(s) 492, 1847, 1862, 2476, 3240, 3415, 3920
BseCI ATCGAT 1 cut(s) 2917
BseJI GATNNNNATC 1 cut(s) 3909
BseLI CCNNNNNNNGG 7 cut(s) 22, 1792, 1867, 2003, 2735, 3010, 3257
BseMI GCAATG 2 cut(s) 3361, 3939
BseNI ACTGG 9 cut(s) 417, 618, 769, 1786, 1955, 2101, 2251, 3034, 3350
BseRI GAGGAG 8 cut(s) 25, 28, 254, 444, 1355, 1492, 1907, 3474
BseSI GKGCMC 2 cut(s) 2287, 2499
BseXI GCAGC 7 cut(s) 148, 893, 1204, 1555, 2226, 3150, 3862
BseYI CCCAGC 1 cut(s) 2072
BsgI GTGCAG 1 cut(s) 336
Bsh1236I CGCG 2 cut(s) 189, 3445
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 9 cut(s) 800, 1782, 1845, 1865, 1912, 2010, 2285, 3230, 3957
BshNI GGYRCC 2 cut(s) 12, 1657
BshVI ATCGAT 1 cut(s) 2917
BsiEI CGRYCG 1 cut(s) 187
BsiHKAI GWGCWC 4 cut(s) 2397, 2499, 3346, 3946
BsiHKCI CYCGRG 1 cut(s) 2761
BsiSI CCGG 3 cut(s) 374, 2131, 3076
BsiWI CGTACG 1 cut(s) 2021
BslFI GGGAC 6 cut(s) 274, 763, 2777, 2986, 3086, 3293
BslI CCNNNNNNNGG 7 cut(s) 22, 1792, 1867, 2003, 2735, 3010, 3257
BsmBI CGTCTC 1 cut(s) 1458
BsmFI GGGAC 6 cut(s) 274, 763, 2777, 2986, 3086, 3293
BsmI GAATGC 2 cut(s) 2413, 3140
BsnI GGCC 9 cut(s) 800, 1782, 1845, 1865, 1912, 2010, 2285, 3230, 3957
Bso31I GGTCTC 1 cut(s) 225
BsoBI CYCGRG 1 cut(s) 2761
Bsp119I TTCGAA 1 cut(s) 398
Bsp120I GGGCCC 1 cut(s) 2283
Bsp1286I GDGCHC 6 cut(s) 762, 2287, 2397, 2499, 3346, 3946
Bsp1720I GCTNAGC 2 cut(s) 2213, 2562
Bsp19I CCATGG 2 cut(s) 1866, 3117
BspACI CCGC 9 cut(s) 187, 217, 531, 1123, 2369, 2729, 2903, 3046, 4006
BspANI GGCC 9 cut(s) 800, 1782, 1845, 1865, 1912, 2010, 2285, 3230, 3957
BspDI ATCGAT 1 cut(s) 2917
BspFNI CGCG 2 cut(s) 189, 3445
BspHI TCATGA 1 cut(s) 2323
BspMAI CTGCAG 3 cut(s) 2848, 3010, 3880
BspPI GGATC 7 cut(s) 687, 700, 1010, 3003, 3769, 3786, 3930
BspQI GCTCTTC 1 cut(s) 3543
BspT104I TTCGAA 1 cut(s) 398
BspT107I GGYRCC 2 cut(s) 12, 1657
BspTNI GGTCTC 1 cut(s) 225
BsrBI CCGCTC 2 cut(s) 3046, 4008
BsrDI GCAATG 2 cut(s) 3361, 3939
BsrFI RCCGGY 1 cut(s) 3075
BsrI ACTGG 9 cut(s) 417, 618, 769, 1786, 1955, 2101, 2251, 3034, 3350
BssAI RCCGGY 1 cut(s) 3075
BssNI GRCGYC 1 cut(s) 13
BssT1I CCWWGG 4 cut(s) 21, 151, 1866, 3117
Bst2UI CCWGG 7 cut(s) 492, 1847, 1862, 2476, 3240, 3415, 3920
Bst6I CTCTTC 8 cut(s) 59, 1051, 1075, 1259, 1998, 2724, 3416, 3543
BstACI GRCGYC 1 cut(s) 13
BstAPI GCANNNNNTGC 1 cut(s) 3014
BstBAI YACGTR 1 cut(s) 3176
BstBI TTCGAA 1 cut(s) 398
BstC8I GCNNGC 2 cut(s) 1127, 1331
BstDSI CCRYGG 2 cut(s) 1866, 3117
BstFNI CGCG 2 cut(s) 189, 3445
BstH2I RGCGCY 1 cut(s) 16
BstHHI GCGC 5 cut(s) 15, 520, 1329, 1610, 3445
BstMCI CGRYCG 1 cut(s) 187
BstNI CCWGG 7 cut(s) 492, 1847, 1862, 2476, 3240, 3415, 3920
BstNSI RCATGY 1 cut(s) 1252
BstPAI GACNNNNGTC 1 cut(s) 1623
BstSCI CCNGG 7 cut(s) 490, 1845, 1860, 2474, 3238, 3413, 3918
BstSFI CTRYAG 7 cut(s) 1458, 2844, 2863, 3006, 3730, 3876, 3926
BstSLI GKGCMC 2 cut(s) 2287, 2499
BstUI CGCG 2 cut(s) 189, 3445
BstV1I GCAGC 7 cut(s) 148, 893, 1204, 1555, 2226, 3150, 3862
BstV2I GAAGAC 2 cut(s) 1455, 2210
BstX2I RGATCY 6 cut(s) 692, 1015, 2995, 3197, 3761, 3922
BstXI CCANNNNNNTGG 1 cut(s) 3775
BstYI RGATCY 6 cut(s) 692, 1015, 2995, 3197, 3761, 3922
Bsu15I ATCGAT 1 cut(s) 2917
Bsu36I CCTNAGG 1 cut(s) 1653
BsuRI GGCC 9 cut(s) 800, 1782, 1845, 1865, 1912, 2010, 2285, 3230, 3957
BsuTUI ATCGAT 1 cut(s) 2917
BtgI CCRYGG 2 cut(s) 1866, 3117
BtrI CACGTC 1 cut(s) 456
BtsI GCAGTG 2 cut(s) 2207, 2497
BtsIMutI CAGTG 6 cut(s) 235, 1793, 2207, 2497, 2872, 3606
Cac8I GCNNGC 2 cut(s) 1127, 1331
CaiI CAGNNNCTG 2 cut(s) 1792, 3884
CciI TCATGA 1 cut(s) 2323
CfoI GCGC 5 cut(s) 15, 520, 1329, 1610, 3445
Cfr10I RCCGGY 1 cut(s) 3075
ClaI ATCGAT 1 cut(s) 2917
CseI GACGC 2 cut(s) 271, 1322
CsiI ACCWGGT 1 cut(s) 3238
CspCI CAANNNNNGTGG 2 cut(s) 2563, 2598
DinI GGCGCC 1 cut(s) 14
DraI TTTAAA 1 cut(s) 1818
DraIII CACNNNGTG 1 cut(s) 527
Eam1104I CTCTTC 8 cut(s) 59, 1051, 1075, 1259, 1998, 2724, 3416, 3543
EarI CTCTTC 8 cut(s) 59, 1051, 1075, 1259, 1998, 2724, 3416, 3543
EciI GGCGGA 1 cut(s) 2718
Ecl136II GAGCTC 2 cut(s) 3344, 3944
Eco130I CCWWGG 4 cut(s) 21, 151, 1866, 3117
Eco147I AGGCCT 2 cut(s) 800, 1782
Eco24I GRGCYC 4 cut(s) 762, 2287, 3346, 3946
Eco31I GGTCTC 1 cut(s) 225
Eco32I GATATC 1 cut(s) 2553
Eco47I GGWCC 6 cut(s) 4, 731, 2791, 3073, 3236, 3464
Eco53kI GAGCTC 2 cut(s) 3344, 3944
Eco57I CTGAAG 6 cut(s) 767, 1467, 1568, 2505, 3524, 3608
Eco81I CCTNAGG 1 cut(s) 1653
Eco88I CYCGRG 1 cut(s) 2761
EcoICRI GAGCTC 2 cut(s) 3344, 3944
EcoO109I RGGNCCY 3 cut(s) 1910, 2284, 2791
EcoRI GAATTC 3 cut(s) 196, 859, 2824
EcoRII CCWGG 7 cut(s) 490, 1845, 1860, 2474, 3238, 3413, 3918
EcoRV GATATC 1 cut(s) 2553
EcoT14I CCWWGG 4 cut(s) 21, 151, 1866, 3117
EcoT38I GRGCYC 4 cut(s) 762, 2287, 3346, 3946
EgeI GGCGCC 1 cut(s) 14
EheI GGCGCC 1 cut(s) 14
ErhI CCWWGG 4 cut(s) 21, 151, 1866, 3117
Esp3I CGTCTC 1 cut(s) 1458
FalI AAGNNNNNCTT 2 cut(s) 1743, 1775
FaqI GGGAC 6 cut(s) 274, 763, 2777, 2986, 3086, 3293
FauNDI CATATG 1 cut(s) 967
FblI GTMKAC 2 cut(s) 933, 2862
Fnu4HI GCNGC 9 cut(s) 162, 531, 882, 1218, 1569, 2240, 3047, 3139, 3876
FriOI GRGCYC 4 cut(s) 762, 2287, 3346, 3946
Fsp4HI GCNGC 9 cut(s) 162, 531, 882, 1218, 1569, 2240, 3047, 3139, 3876
FspBI CTAG 7 cut(s) 813, 1238, 1605, 2108, 3263, 3339, 3857
FspI TGCGCA 1 cut(s) 1328
GlaI GCGC 5 cut(s) 14, 519, 1328, 1609, 3444
GluI GCNGC 9 cut(s) 162, 531, 882, 1218, 1569, 2240, 3047, 3139, 3876
GsaI CCCAGC 1 cut(s) 2076
GsuI CTGGAG 4 cut(s) 1358, 1695, 1769, 2804
HaeII RGCGCY 1 cut(s) 16
HaeIII GGCC 9 cut(s) 800, 1782, 1845, 1865, 1912, 2010, 2285, 3230, 3957
HapII CCGG 3 cut(s) 374, 2131, 3076
HgaI GACGC 2 cut(s) 271, 1322
HhaI GCGC 5 cut(s) 15, 520, 1329, 1610, 3445
Hin1I GRCGYC 1 cut(s) 13
Hin6I GCGC 5 cut(s) 13, 518, 1327, 1608, 3443
HinP1I GCGC 5 cut(s) 13, 518, 1327, 1608, 3443
HincII GTYRAC 3 cut(s) 1602, 1725, 3546
HindII GTYRAC 3 cut(s) 1602, 1725, 3546
HindIII AAGCTT 2 cut(s) 2523, 3583
HpaI GTTAAC 2 cut(s) 1602, 3546
HpaII CCGG 3 cut(s) 374, 2131, 3076
HphI GGTGA 6 cut(s) 628, 1112, 1198, 2714, 2892, 3430
Hpy99I CGWCG 1 cut(s) 2445
HpyCH4IV ACGT 7 cut(s) 455, 1488, 1721, 2751, 2834, 2972, 3175
HpySE526I ACGT 7 cut(s) 455, 1488, 1721, 2751, 2834, 2972, 3175
Hsp92I GRCGYC 1 cut(s) 13
HspAI GCGC 5 cut(s) 13, 518, 1327, 1608, 3443
KasI GGCGCC 1 cut(s) 12
KspAI GTTAAC 2 cut(s) 1602, 3546
LguI GCTCTTC 1 cut(s) 3543
Lsp1109I GCAGC 7 cut(s) 148, 893, 1204, 1555, 2226, 3150, 3862
LweI GCATC 9 cut(s) 496, 1105, 1129, 1567, 1833, 2059, 2596, 3177, 3690
MabI ACCWGGT 1 cut(s) 3238
MaeI CTAG 7 cut(s) 813, 1238, 1605, 2108, 3263, 3339, 3857
MaeII ACGT 7 cut(s) 455, 1488, 1721, 2751, 2834, 2972, 3175
MaeIII GTNAC 5 cut(s) 616, 1409, 1717, 2488, 3169
MbiI CCGCTC 2 cut(s) 3046, 4008
MflI RGATCY 6 cut(s) 692, 1015, 2995, 3197, 3761, 3922
MhlI GDGCHC 6 cut(s) 762, 2287, 2397, 2499, 3346, 3946
Mly113I GGCGCC 1 cut(s) 13
MlyI GAGTC 5 cut(s) 20, 785, 2936, 3475, 3747
MmeI TCCRAC 5 cut(s) 72, 207, 2724, 2941, 3938
MroXI GAANNNNTTC 5 cut(s) 306, 2126, 2429, 2874, 3031
MslI CAYNNNNRTG 4 cut(s) 2592, 2700, 3107, 3773
MspA1I CMGCKG 3 cut(s) 533, 884, 3875
MspI CCGG 3 cut(s) 374, 2131, 3076
MspR9I CCNGG 7 cut(s) 492, 1847, 1862, 2476, 3240, 3415, 3920
Mva1269I GAATGC 2 cut(s) 2413, 3140
MvaI CCWGG 7 cut(s) 492, 1847, 1862, 2476, 3240, 3415, 3920
MvnI CGCG 2 cut(s) 189, 3445
NarI GGCGCC 1 cut(s) 13
NcoI CCATGG 2 cut(s) 1866, 3117
NdeI CATATG 1 cut(s) 967
NmeAIII GCCGAG 2 cut(s) 660, 2494
NmuCI GTSAC 2 cut(s) 616, 1409
NsbI TGCGCA 1 cut(s) 1328
NspI RCATGY 1 cut(s) 1252
NspV TTCGAA 1 cut(s) 398
OliI CACNNNNGTG 1 cut(s) 2700
PagI TCATGA 1 cut(s) 2323
PasI CCCWGGG 1 cut(s) 3414
PceI AGGCCT 2 cut(s) 800, 1782
PciSI GCTCTTC 1 cut(s) 3543
PctI GAATGC 2 cut(s) 2413, 3140
PdmI GAANNNNTTC 5 cut(s) 306, 2126, 2429, 2874, 3031
PfeI GAWTC 9 cut(s) 335, 370, 592, 1529, 2870, 3128, 3509, 3611, 3910
Pfl23II CGTACG 1 cut(s) 2021
PflFI GACNNNGTC 1 cut(s) 3791
PflMI CCANNNNNTGG 2 cut(s) 1792, 1867
PkrI GCNGC 9 cut(s) 163, 532, 883, 1219, 1570, 2241, 3048, 3140, 3877
PleI GAGTC 5 cut(s) 20, 784, 2936, 3475, 3747
PluTI GGCGCC 1 cut(s) 16
PpsI GAGTC 5 cut(s) 20, 784, 2936, 3475, 3747
Ppu21I YACGTR 1 cut(s) 3176
PpuMI RGGWCCY 1 cut(s) 2791
PshAI GACNNNNGTC 1 cut(s) 1623
Psp124BI GAGCTC 2 cut(s) 3346, 3946
Psp1406I AACGTT 2 cut(s) 2834, 2972
Psp5II RGGWCCY 1 cut(s) 2791
Psp6I CCWGG 7 cut(s) 490, 1845, 1860, 2474, 3238, 3413, 3918
PspFI CCCAGC 1 cut(s) 2072
PspGI CCWGG 7 cut(s) 490, 1845, 1860, 2474, 3238, 3413, 3918
PspLI CGTACG 1 cut(s) 2021
PspOMI GGGCCC 1 cut(s) 2283
PspPPI RGGWCCY 1 cut(s) 2791
PsrI GAACNNNNNNTAC 4 cut(s) 379, 411, 972, 1004
PstI CTGCAG 3 cut(s) 2848, 3010, 3880
PstNI CAGNNNCTG 2 cut(s) 1792, 3884
PsuI RGATCY 6 cut(s) 692, 1015, 2995, 3197, 3761, 3922
PsyI GACNNNGTC 1 cut(s) 3791
PvuII CAGCTG 2 cut(s) 884, 3875
RseI CAYNNNNRTG 4 cut(s) 2592, 2700, 3107, 3773
SacI GAGCTC 2 cut(s) 3346, 3946
SapI GCTCTTC 1 cut(s) 3543
SatI GCNGC 9 cut(s) 162, 531, 882, 1218, 1569, 2240, 3047, 3139, 3876
SchI GAGTC 5 cut(s) 20, 785, 2936, 3475, 3747
ScrFI CCNGG 7 cut(s) 492, 1847, 1862, 2476, 3240, 3415, 3920
SduI GDGCHC 6 cut(s) 762, 2287, 2397, 2499, 3346, 3946
SexAI ACCWGGT 1 cut(s) 3238
SfaNI GCATC 9 cut(s) 496, 1105, 1129, 1567, 1833, 2059, 2596, 3177, 3690
SfcI CTRYAG 7 cut(s) 1458, 2844, 2863, 3006, 3730, 3876, 3926
SfoI GGCGCC 1 cut(s) 14
SfuI TTCGAA 1 cut(s) 398
SinI GGWCC 6 cut(s) 4, 731, 2791, 3073, 3236, 3464
SmiMI CAYNNNNRTG 4 cut(s) 2592, 2700, 3107, 3773
SmlI CTYRAG 5 cut(s) 62, 1572, 3146, 3652, 3713
SmoI CTYRAG 5 cut(s) 62, 1572, 3146, 3652, 3713
SseBI AGGCCT 2 cut(s) 800, 1782
SsiI CCGC 9 cut(s) 187, 217, 531, 1123, 2369, 2729, 2903, 3046, 4006
SspDI GGCGCC 1 cut(s) 12
SspI AATATT 5 cut(s) 1519, 2623, 2925, 3298, 3709
SspMI CTAG 7 cut(s) 813, 1238, 1605, 2108, 3263, 3339, 3857
SstI GAGCTC 2 cut(s) 3346, 3946
StuI AGGCCT 2 cut(s) 800, 1782
StyD4I CCNGG 7 cut(s) 490, 1845, 1860, 2474, 3238, 3413, 3918
StyI CCWWGG 4 cut(s) 21, 151, 1866, 3117
TaiI ACGT 7 cut(s) 458, 1491, 1724, 2754, 2837, 2975, 3178
TatI WGTACW 2 cut(s) 315, 3839
TauI GCSGC 2 cut(s) 533, 3049
TfiI GAWTC 9 cut(s) 335, 370, 592, 1529, 2870, 3128, 3509, 3611, 3910
TscAI CASTG 6 cut(s) 235, 1793, 2214, 2504, 2872, 3613
TseFI GTSAC 2 cut(s) 616, 1409
TseI GCWGC 7 cut(s) 161, 881, 1217, 1568, 2239, 3138, 3875
Tsp45I GTSAC 2 cut(s) 616, 1409
TspGWI ACGGA 4 cut(s) 287, 1986, 2429, 3299
TspRI CASTG 6 cut(s) 235, 1793, 2214, 2504, 2872, 3613
Tth111I GACNNNGTC 1 cut(s) 3791
Van91I CCANNNNNTGG 2 cut(s) 1792, 1867
VneI GTGCAC 1 cut(s) 2495
VpaK11BI GGWCC 6 cut(s) 4, 731, 2791, 3073, 3236, 3464
XapI RAATTY 6 cut(s) 196, 859, 2604, 2713, 2824, 3555
XceI RCATGY 1 cut(s) 1252
XcmI CCANNNNNNNNNTGG 2 cut(s) 1864, 1873
XmiI GTMKAC 2 cut(s) 933, 2862
XmnI GAANNNNTTC 5 cut(s) 306, 2126, 2429, 2874, 3031
XspI CTAG 7 cut(s) 813, 1238, 1605, 2108, 3263, 3339, 3857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.