MD03G1136600.v1.1

DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005736
Physical Location & Seq
Reverse (-)
13709387 .. 13722733
13347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1136600.v1.1.491

Sequence Viewer

Length: 4458 bp
ATGGACCACCGGAGGCAACCCAAGGATTCTCTCTCATACTCCAACCTTTTCAATCTTGAGGTTTATGGAAGTGTTGGTAACGGGATGATGTCTGACAGAGAACTGAGTTCAGTGAAAAAAAGAAGGCGATCCCAAATTAGTGACTGTGAGGACGATGACAGTTATTACGGGACACGCATTACAGAAGAAAGGTATCGATCAATGCTTGGAGAACACATTCAGAAGTACAAAAGGAGGTTCAAGGATTCCTCGTCAAGTCCTGCACCAATTCAGATGGGGATTCCAGTTCCAAAGGGTAATAAGGGCTTAAAATCTAGGAAACTGGGCAATGAGCAGCAGGGGAGGTTATATGAAATGGAAACCTCGTCAGAGTGGCTCAATGATTCTAATCCTCAAAAATCAGTGAACCACCATGATGCAGATTTGGCACCAGAATGTGGCACCGATAGAATAATGTATGAACCTCCTTATCTGGATATAGGAGATGGTATAACTCACAGGATTCCACCAATTTATGATAAGCTGGTGTCATCTTTGCACTTACCGAGCTTTTCAGATTTTCGGGTGGAGGAAGTTTACTTAAGAGGCACGTTGGACTTGGGATCCTTGGCAGAAATGATGACTAGAGACAAAAGGTTGGGCCCTAAAGGCCAGGCAGGGATGGGCGATCCCCATCCCCAGTATGAATCCCTTCAAGCTAGACTTAAGGCATTGCCCACTTCGAATTCAGCTCAAAAGTTCAGCCTCAAGGTCTTTGATATTGGGTTAAACTCTTCCATCCCAGAGGGTGCAGCTGGAAATATAAAGAGATATATTTTGTCTGAAGGTGGTGTTTTACAGGTCTACTATGTGAAAGTTTTGGAGAAAGGAGACACATATGAGATCATTGAGCGAAGTCTATCAAAGAAGCAAAAGGTTATTAAAGACCCTTCTGTGATTGAGAAGGAGGAAATGGACAAGATTGGGAAAGTTTGGGCAAACATAGTAAGAAGAGACATGCCAAGGCATCATAGGATTTTTTCGAGTTTTCACCAGAGGCAACTAGGTTATGCTAAGAGGGTATCAGAGAACTGTCAAAGAGAGGTGAAATTGAAGGTCAGTAGATCTCTTAAATTGATGAGGGGTGCCGCAATTCGTACAAGGAAACTAGCCAGAGATATGCTACTATTTTGGAAGAGAATAGATAAAGAGATGGCGGAAGTGAGGAAGAAAGAGGAGAAAGAAGCAGCTGAAGCTTTGAGGCGCGAACAGGAGCTTCGAGAAGCAAAGAGACAACAGCAAAGGCTCAATTTTCTTATACAGCAAACGGAACTTTACAGTCACTTTATGCAGAACAAGTCAAGTTCACAGCCATCTGAAGATCTGCCTGTAGGTGAGGGGGAAAAAAAGGACAAAAAAGCATTTTTGAGCTCCTCAGATGATGAAACTAATGAGGAAGAAGACCCTGAGGAGGCTGAACTGAAGGAGGCCGCTTTTAAAGCAGCTCAAGAGGCAGTTTCAAAGCAGAAAAAGATAACCAGTGAATTTGATAATGAGTATTTGAAGCTATGTGAAGATGCTGAACCTGAGGCAGCACAGGATGTTGCTGGAGCTAGTAGCATAGATCTACATAACCCTTCCACAATGCCTGTAACATCAACTGTTCAGACACCGGAGTTGTTCAAAGGATCCCTTAAAGAATATCAGCTTAAAGGTCTCCAGTGGCTGGTTAATTGTTATGAGCAGGGTTTAAATGGCATCCTTGCTGATGAAATGGGCCTTGGAAAGACCATTCAGGCCATGGCATTTTTGGCTCATTTGGCTGAGGAGAAAAATATTTGGGGGCCTTTTCTGGTTGTTGCTCCTGCCTCTGTCTTGAACAACTGGGCAGATGAAATTAGTCGTTTCTGCCCTGACTTAAAAACTCTTCCATACTGGGGAGGAGTCAACGAGCGTCAGGTACTTAGGAAAAAGATCACCTCTAAAAAACTTTACCGCAGGGATGCTGGATTTCATATTCTCATTACCAGCTACCAGCTGCTAGTGGCTGATGGAAAGTATTTTAAACGTGTGAAATGGCAGTATATGGTATTAGATGAAGCCCAAGCAATCAAAAGTTCCAACAGCATAAGATGGAAGACACTTCTAGGCTTTAATTGTCGAAATCGCTTGCTGCTTACCGGTACACCTATACAAAATAATATGGCAGAGTTGTGGGCTCTACTACATTTCATTATGCCAACCTTATTTGACAGCCATGAACAATTCAATGAGTGGTTTTCAAAAGGAATTGAAAACCATGCTGAACATGGGGGTACTTTGAACGAGCACCAGCTTAACAGATTGCATTCCATATTAAAGCCATTCATGCTACGCCGAGTTAAAGCAGATGTGGTTTCTGAGCTGACCAGGAAAACTGAGGTTACTGTGCACTGCAAGTTGAGCCCTCGGCAACAAGCTTTTTATCAAGCCATCAAGAACAAAATATCTCTTGCTGAGTTGTTTGACAGCAATCACGGTCATCTTAGTGAGAAGAAAATTTTGAATTTGATGAATATTGTCATTCAGCTGAGAAAGGTCTGCAACCATCCAGAGTTATTTGAGAGGAACGAAGGAGTTACATATCTATACTTTAGTGAGATACCAAATTCTCTTCTAGCTCCTCCATTTGGGGAGTTGGAGGATGTGCACTACTCAGGAGGTCAAAATCCTATAACATACTCGATCCCTAAACTTTTCTATCAAGAAATTCTTCGGAGCTCTGAAACTTTTTGCTCAGCAGTTCGACATGGTGTCTACAAAGAATCATTTGAGAAATATTTTAACATATTTTCACCGGGAAATGTTTATCAATCAACACTCTTGCAAGAGGATAGCTCATCTGTTGGAAGTGGAACTTTTGGCTTTACTCGTATGATGGATCTGTCCCCTGCTGAGGTGGCATTTCTAGGAACTGGTTCTTTTATGGAGCGACTGATGTTTTCCATTGTGAGATGGTACCGACAATTTTTGGATGGAATTGTGGACTCACTAATGGAAACCATGAATGATGATTCTGAATGCAGTCATCTTGAAAGTGGACAAGTGAGAGCTGTTACACGAATGTTGCTGATGCCTTCTAGATCTGTAACTAACTTTCTTCAAAAGAAATTGGCTACAGGACCTGGTGATGATCCATTCGAGGCTTTGGTTGTCTCTCCTAGGGATAGGCTACTCTCTAACACTAGGCTTCTTCATTCAGCATACACATTCATCCCACGAACTAGAGCTCCCCCAGTCACTGTCTGTTGCTCAGACAGAAACTTTGCTTACAAAATGATTGAAGAACTGCAATACCCTTGGGTGAAGAGGTTGTTTACTGGGTTTGCACGCACATCCGACTTTAGTGGACCCAGAAAACCTGAGACTCCACATCATTTAATACAAGAGATTGATTCTGAACTGCCTGTTTCACATCCTGCTCTTCAGTTAACATACAAGATTTTTGGGTCTTGTCCTCCCATGCAAAGCTTTGACCCAGCAAAATTGCTCACCGATTCTGGGAAACTTCAGACGCTTGATATATTGTTGAAACGCTTGAGGGCAGACAACCACCGTGTTCTCTTGTTTGCTCAAATGACGAAGATGCTGAATATTCTTGAGGACTACATGAACTACAGGAAATATAAATACTTACGACTTGATGGATCCTCCACCATTATGGATCGGCGCGACATGGTTAGGGACTTCCAGCAACGAAGTGATATTTTTGTGTTCTTGCTGAGTACAAGAGCTGGTGGACTTGGCATTAACTTGACAGCTGCCGATACTGTCATATTTTATGAAAGTGATTGGAATCCAACCCTGGATCTACAGGCAATGGATAGAGCTCATCGGTTGGGTCAGACAAAAGATGTTACCGTCTACCGACTAATTTGTAAAGAAACAGTTGAAGAGAAGATTCTGCAGCGAGCAAGTCAGAAAAATACTGTCCAGCAGCTTGTAATGATGGGTGGTCATGTTCAGGGTGATCTTTTGGCTCCTGAGGATGTTGTATCTTTACTTCTGGATGATGCTCAGTTGGAGCAGAAATTAAGAGAAATTCCGTTGCAGGTTAAGGATAGACAAAAAAAGAAACAAACAAAGGGTATCCGGGTGGATGCAGAAGGGGATGCCTCTTTAGAAGATTTAACAAACCCTGCATCCGCACCTCAGGGCACCGGATATGAGGACTCTCCAGATACGGAAAAAGCAAAATCTAATAATAAAACGAGGAAAGCTGCAACTGGCAGGCAAACTACAAGGACGAAGAATTCACAAAGCATGGACGAACTTGATAGATATGAGTTGGATGATACCTTACCAAATACTGACCCACAAGCTACGAAACCCAAGAGGCCAAAGAGGTCAAACAAGAGTGTGAACGAAAATCTTGAACCGGCATTTACTGCCACACTTTCTGCGGTTCCAGAACAGACCCAGTATCCACCAAGGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBINO Helicase_C SNF2_N

Protein Analysis

1486

Amino Acids

169.61

Weight (kDa)

8.48

Isoelectric Point (pI)

48.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBINO PF13892 319 - 449 4.8e-46 DNA-binding domain
ResIII PF04851 557 - 724 1.3e-07 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 561 - 859 1.8e-85 SNF2-related domain
Helicase_C PF00271 1177 - 1287 2.7e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 4060
Acc65I GGTACC 1 cut(s) 2976
AccB1I GGYRCC 5 cut(s) 427, 440, 1124, 2976, 4175
AccB7I CCANNNNNTGG 2 cut(s) 437, 1705
AccI GTMKAC 3 cut(s) 843, 2775, 3882
AccII CGCG 2 cut(s) 1245, 3690
AciI CCGC 6 cut(s) 1128, 1196, 1470, 1975, 4164, 4421
AcsI RAATTY 8 cut(s) 724, 1523, 2517, 2524, 2626, 2727, 4059, 4270
AcuI CTGAAG 6 cut(s) 843, 1251, 1377, 1481, 3428, 3512
AfaI GTAC 7 cut(s) 227, 1138, 1941, 2164, 2296, 2978, 3745
AfiI CCNNNNNNNGG 7 cut(s) 437, 1450, 1705, 1916, 2648, 2914, 3519
AflII CTTAAG 2 cut(s) 580, 704
AflIII ACRYGT 1 cut(s) 2047
AgeI ACCGGT 1 cut(s) 2159
AhdI GACNNNNNGTC 1 cut(s) 2771
AjnI CCWGG 4 cut(s) 651, 2387, 3142, 3822
AjuI GAANNNNNNNTTGG 2 cut(s) 1743, 1775
AloI GAACNNNNNNTCC 2 cut(s) 3232, 3264
Alw21I GWGCWC 7 cut(s) 1412, 2310, 2412, 2670, 2741, 3250, 3850
Alw26I GTCTC 7 cut(s) 621, 864, 987, 1264, 1700, 3178, 3377
Alw44I GTGCAC 2 cut(s) 2408, 2666
AlwNI CAGNNNCTG 3 cut(s) 1705, 3143, 3260
AoxI GGCC 7 cut(s) 640, 649, 1467, 1756, 1776, 1823, 4355
ApaI GGGCCC 1 cut(s) 644
ApaLI GTGCAC 2 cut(s) 2408, 2666
ApoI RAATTY 8 cut(s) 724, 1523, 2517, 2524, 2626, 2727, 4059, 4270
ArsI GACNNNNNNTTYG 2 cut(s) 3266, 3298
AsiGI ACCGGT 1 cut(s) 2159
Asp700I GAANNNNTTC 4 cut(s) 216, 690, 2730, 2935
Asp718I GGTACC 1 cut(s) 2976
AspA2I CCTAGG 1 cut(s) 3179
AspLEI GCGC 2 cut(s) 1245, 3690
AspS9I GGNCC 7 cut(s) 4, 640, 641, 1756, 1823, 3140, 3368
AsuC2I CCSGG 2 cut(s) 2817, 4112
AsuHPI GGTGA 9 cut(s) 1022, 1096, 1385, 1948, 2805, 3158, 3334, 3502, 3998
AsuII TTCGAA 1 cut(s) 722
AvaII GGWCC 3 cut(s) 4, 3140, 3368
AvrII CCTAGG 1 cut(s) 3179
AxyI CCTNAGG 4 cut(s) 1446, 1566, 4002, 4170
BaeGI GKGCMC 4 cut(s) 644, 2412, 2670, 4178
BamHI GGATCC 3 cut(s) 602, 1667, 3665
BanI GGYRCC 5 cut(s) 427, 440, 1124, 2976, 4175
BanII GRGCYC 7 cut(s) 644, 1412, 2200, 2426, 2741, 3250, 3850
BarI GAAGNNNNNNTAC 2 cut(s) 4005, 4037
BbsI GAAGAC 2 cut(s) 1446, 2123
Bbv12I GWGCWC 7 cut(s) 1412, 2310, 2412, 2670, 2741, 3250, 3850
BbvCI CCTCAGC 2 cut(s) 1803, 2913
BciT130I CCWGG 4 cut(s) 653, 2389, 3144, 3824
BciVI GTATCC 2 cut(s) 4118, 4452
BcnI CCSGG 2 cut(s) 2817, 4112
BcoDI GTCTC 7 cut(s) 621, 864, 987, 1264, 1700, 3178, 3377
BfmI CTRYAG 5 cut(s) 1368, 3135, 3634, 3830, 3923
BfrI CTTAAG 2 cut(s) 580, 704
BfuAI ACCTGC 1 cut(s) 4060
BfuI GTATCC 2 cut(s) 4118, 4452
BglI GCCNNNNNGGC 1 cut(s) 648
BglII AGATCT 4 cut(s) 1103, 1360, 1603, 3101
BlnI CCTAGG 1 cut(s) 3179
BlpI GCTNAGC 1 cut(s) 2755
Bme1390I CCNGG 6 cut(s) 653, 2389, 2817, 3144, 3824, 4112
Bme18I GGWCC 3 cut(s) 4, 3140, 3368
BmeRI GACNNNNNGTC 1 cut(s) 2771
BmgT120I GGNCC 7 cut(s) 4, 640, 641, 1756, 1823, 3140, 3368
BmrFI CCNGG 6 cut(s) 653, 2389, 2817, 3144, 3824, 4112
BmrI ACTGGG 7 cut(s) 332, 673, 1873, 1924, 3248, 3348, 4432
BmuI ACTGGG 7 cut(s) 332, 673, 1873, 1924, 3248, 3348, 4432
BpiI GAAGAC 2 cut(s) 1446, 2123
BplI GAGNNNNNCTC 2 cut(s) 2840, 2872
BpmI CTGGAG 3 cut(s) 1608, 1682, 4179
Bpu10I CCTNAGC 2 cut(s) 1803, 2913
Bpu1102I GCTNAGC 1 cut(s) 2755
Bpu14I TTCGAA 1 cut(s) 722
BpuEI CTTGAG 5 cut(s) 77, 731, 1470, 3577, 3638
BpuMI CCSGG 2 cut(s) 2817, 4112
Bsa29I ATCGAT 1 cut(s) 196
BsaBI GATNNNNATC 3 cut(s) 387, 672, 3813
BsaI GGTCTC 1 cut(s) 1700
BsaWI WCCGGW 4 cut(s) 9, 1651, 2159, 4178
BsaXI ACNNNNNCTCC 2 cut(s) 3232, 3262
Bsc4I CCNNNNNNNGG 7 cut(s) 437, 1450, 1705, 1916, 2648, 2914, 3519
Bse118I RCCGGY 2 cut(s) 2159, 4396
Bse21I CCTNAGG 4 cut(s) 1446, 1566, 4002, 4170
Bse3DI GCAATG 3 cut(s) 334, 710, 3843
Bse8I GATNNNNATC 3 cut(s) 387, 672, 3813
BseBI CCWGG 4 cut(s) 653, 2389, 3144, 3824
BseCI ATCGAT 1 cut(s) 196
BseJI GATNNNNATC 3 cut(s) 387, 672, 3813
BseLI CCNNNNNNNGG 7 cut(s) 437, 1450, 1705, 1916, 2648, 2914, 3519
BseMI GCAATG 3 cut(s) 334, 710, 3843
BseRI GAGGAG 6 cut(s) 1229, 1402, 1463, 1820, 1935, 2631
BseSI GKGCMC 4 cut(s) 644, 2412, 2670, 4178
BseYI CCCAGC 1 cut(s) 3496
BsgI GTGCAG 2 cut(s) 246, 810
Bsh1236I CGCG 2 cut(s) 1245, 3690
BshFI GGCC 7 cut(s) 642, 651, 1469, 1758, 1778, 1825, 4357
BshNI GGYRCC 5 cut(s) 427, 440, 1124, 2976, 4175
BshTI ACCGGT 1 cut(s) 2159
BshVI ATCGAT 1 cut(s) 196
BsiHKAI GWGCWC 7 cut(s) 1412, 2310, 2412, 2670, 2741, 3250, 3850
BsiSI CCGG 7 cut(s) 10, 1652, 2160, 2816, 4111, 4179, 4397
BslFI GGGAC 3 cut(s) 184, 2890, 3716
BslI CCNNNNNNNGG 7 cut(s) 437, 1450, 1705, 1916, 2648, 2914, 3519
BsmAI GTCTC 7 cut(s) 621, 864, 987, 1264, 1700, 3178, 3377
BsmFI GGGAC 3 cut(s) 184, 2890, 3716
BsmI GAATGC 2 cut(s) 2326, 3044
BsnI GGCC 7 cut(s) 642, 651, 1469, 1758, 1778, 1825, 4357
Bso31I GGTCTC 1 cut(s) 1700
Bsp119I TTCGAA 1 cut(s) 722
Bsp120I GGGCCC 1 cut(s) 640
Bsp1720I GCTNAGC 1 cut(s) 2755
Bsp19I CCATGG 1 cut(s) 1779
BspACI CCGC 6 cut(s) 1128, 1196, 1470, 1975, 4164, 4421
BspANI GGCC 7 cut(s) 642, 651, 1469, 1758, 1778, 1825, 4357
BspDI ATCGAT 1 cut(s) 196
BspFNI CGCG 2 cut(s) 1245, 3690
BspMAI CTGCAG 1 cut(s) 3927
BspMI ACCTGC 1 cut(s) 4060
BspQI GCTCTTC 1 cut(s) 3447
BspT104I TTCGAA 1 cut(s) 722
BspT107I GGYRCC 5 cut(s) 427, 440, 1124, 2976, 4175
BspTI CTTAAG 2 cut(s) 580, 704
BspTNI GGTCTC 1 cut(s) 1700
BsrDI GCAATG 3 cut(s) 334, 710, 3843
BsrFI RCCGGY 2 cut(s) 2159, 4396
BssAI RCCGGY 2 cut(s) 2159, 4396
BssT1I CCWWGG 8 cut(s) 21, 606, 1001, 1759, 1779, 3179, 3317, 4448
Bst2UI CCWGG 4 cut(s) 653, 2389, 3144, 3824
Bst6I CTCTTC 8 cut(s) 778, 985, 1169, 1911, 2637, 3320, 3447, 3906
BstAFI CTTAAG 2 cut(s) 580, 704
BstAPI GCANNNNNTGC 1 cut(s) 4406
BstBI TTCGAA 1 cut(s) 722
BstC8I GCNNGC 4 cut(s) 2150, 3349, 3930, 4250
BstDSI CCRYGG 1 cut(s) 1779
BstFNI CGCG 2 cut(s) 1245, 3690
BstHHI GCGC 2 cut(s) 1245, 3690
BstMAI GTCTC 7 cut(s) 621, 864, 987, 1264, 1700, 3178, 3377
BstNI CCWGG 4 cut(s) 653, 2389, 3144, 3824
BstNSI RCATGY 1 cut(s) 1000
BstSCI CCNGG 6 cut(s) 651, 2387, 2815, 3142, 3822, 4110
BstSFI CTRYAG 5 cut(s) 1368, 3135, 3634, 3830, 3923
BstSLI GKGCMC 4 cut(s) 644, 2412, 2670, 4178
BstUI CGCG 2 cut(s) 1245, 3690
BstV2I GAAGAC 2 cut(s) 1446, 2123
BstX2I RGATCY 9 cut(s) 602, 1103, 1360, 1603, 1667, 2899, 3101, 3665, 3826
BstXI CCANNNNNNTGG 1 cut(s) 3679
BstYI RGATCY 9 cut(s) 602, 1103, 1360, 1603, 1667, 2899, 3101, 3665, 3826
Bsu15I ATCGAT 1 cut(s) 196
Bsu36I CCTNAGG 4 cut(s) 1446, 1566, 4002, 4170
BsuI GTATCC 2 cut(s) 4118, 4452
BsuRI GGCC 7 cut(s) 642, 651, 1469, 1758, 1778, 1825, 4357
BsuTUI ATCGAT 1 cut(s) 196
BtgI CCRYGG 1 cut(s) 1779
BtsI GCAGTG 1 cut(s) 2410
BtsIMutI CAGTG 6 cut(s) 117, 408, 1525, 1706, 2410, 3258
BveI ACCTGC 1 cut(s) 4060
Cac8I GCNNGC 4 cut(s) 2150, 3349, 3930, 4250
CaiI CAGNNNCTG 3 cut(s) 1705, 3143, 3260
CfoI GCGC 2 cut(s) 1245, 3690
Cfr10I RCCGGY 2 cut(s) 2159, 4396
Cfr13I GGNCC 7 cut(s) 4, 640, 641, 1756, 1823, 3140, 3368
ClaI ATCGAT 1 cut(s) 196
CseI GACGC 2 cut(s) 1922, 3541
CsiI ACCWGGT 1 cut(s) 3142
Csp6I GTAC 7 cut(s) 226, 1137, 1940, 2163, 2295, 2977, 3744
CspAI ACCGGT 1 cut(s) 2159
CviQI GTAC 7 cut(s) 226, 1137, 1940, 2163, 2295, 2977, 3744
DraI TTTAAA 3 cut(s) 1477, 1731, 2044
DriI GACNNNNNGTC 1 cut(s) 2771
Eam1104I CTCTTC 8 cut(s) 778, 985, 1169, 1911, 2637, 3320, 3447, 3906
Eam1105I GACNNNNNGTC 1 cut(s) 2771
EarI CTCTTC 8 cut(s) 778, 985, 1169, 1911, 2637, 3320, 3447, 3906
EciI GGCGGA 1 cut(s) 1211
Ecl136II GAGCTC 4 cut(s) 1410, 2739, 3248, 3848
Eco130I CCWWGG 8 cut(s) 21, 606, 1001, 1759, 1779, 3179, 3317, 4448
Eco24I GRGCYC 7 cut(s) 644, 1412, 2200, 2426, 2741, 3250, 3850
Eco31I GGTCTC 1 cut(s) 1700
Eco47I GGWCC 3 cut(s) 4, 3140, 3368
Eco53kI GAGCTC 4 cut(s) 1410, 2739, 3248, 3848
Eco57I CTGAAG 6 cut(s) 843, 1251, 1377, 1481, 3428, 3512
Eco81I CCTNAGG 4 cut(s) 1446, 1566, 4002, 4170
EcoICRI GAGCTC 4 cut(s) 1410, 2739, 3248, 3848
EcoO109I RGGNCCY 3 cut(s) 641, 1823, 3140
EcoRI GAATTC 2 cut(s) 724, 4270
EcoRII CCWGG 4 cut(s) 651, 2387, 3142, 3822
EcoT14I CCWWGG 8 cut(s) 21, 606, 1001, 1759, 1779, 3179, 3317, 4448
EcoT38I GRGCYC 7 cut(s) 644, 1412, 2200, 2426, 2741, 3250, 3850
ErhI CCWWGG 8 cut(s) 21, 606, 1001, 1759, 1779, 3179, 3317, 4448
FalI AAGNNNNNCTT 8 cut(s) 687, 719, 1656, 1688, 2715, 2747, 2860, 2892
FaqI GGGAC 3 cut(s) 184, 2890, 3716
FauNDI CATATG 1 cut(s) 877
FblI GTMKAC 3 cut(s) 843, 2775, 3882
FriOI GRGCYC 7 cut(s) 644, 1412, 2200, 2426, 2741, 3250, 3850
GlaI GCGC 2 cut(s) 1244, 3689
GsaI CCCAGC 1 cut(s) 3500
GsuI CTGGAG 3 cut(s) 1608, 1682, 4179
HaeIII GGCC 7 cut(s) 642, 651, 1469, 1758, 1778, 1825, 4357
HapII CCGG 7 cut(s) 10, 1652, 2160, 2816, 4111, 4179, 4397
HgaI GACGC 2 cut(s) 1922, 3541
HhaI GCGC 2 cut(s) 1245, 3690
Hin6I GCGC 2 cut(s) 1243, 3688
HinP1I GCGC 2 cut(s) 1243, 3688
HincII GTYRAC 2 cut(s) 1927, 3450
HindII GTYRAC 2 cut(s) 1927, 3450
HindIII AAGCTT 3 cut(s) 1233, 2436, 3487
HpaI GTTAAC 1 cut(s) 3450
HpaII CCGG 7 cut(s) 10, 1652, 2160, 2816, 4111, 4179, 4397
HphI GGTGA 9 cut(s) 1022, 1096, 1385, 1948, 2805, 3158, 3334, 3502, 3998
HpyCH4IV ACGT 2 cut(s) 590, 2047
HpySE526I ACGT 2 cut(s) 590, 2047
HspAI GCGC 2 cut(s) 1243, 3688
KpnI GGTACC 1 cut(s) 2980
KspAI GTTAAC 1 cut(s) 3450
LguI GCTCTTC 1 cut(s) 3447
MabI ACCWGGT 1 cut(s) 3142
MaeII ACGT 2 cut(s) 590, 2047
MflI RGATCY 9 cut(s) 602, 1103, 1360, 1603, 1667, 2899, 3101, 3665, 3826
MlyI GAGTC 4 cut(s) 1932, 2999, 3379, 4184
MmeI TCCRAC 9 cut(s) 66, 573, 2124, 2637, 2845, 3381, 3842, 4020, 4287
MroXI GAANNNNTTC 4 cut(s) 216, 690, 2730, 2935
MslI CAYNNNNRTG 4 cut(s) 414, 433, 2505, 3677
MspA1I CMGCKG 5 cut(s) 794, 1229, 2017, 2548, 3779
MspCI CTTAAG 2 cut(s) 580, 704
MspI CCGG 7 cut(s) 10, 1652, 2160, 2816, 4111, 4179, 4397
MspR9I CCNGG 6 cut(s) 653, 2389, 2817, 3144, 3824, 4112
Mva1269I GAATGC 2 cut(s) 2326, 3044
MvaI CCWGG 4 cut(s) 653, 2389, 3144, 3824
MvnI CGCG 2 cut(s) 1245, 3690
NciI CCSGG 2 cut(s) 2817, 4112
NcoI CCATGG 1 cut(s) 1779
NdeI CATATG 1 cut(s) 877
NmeAIII GCCGAG 2 cut(s) 2381, 2407
NmuCI GTSAC 3 cut(s) 140, 1319, 3256
NspI RCATGY 1 cut(s) 1000
NspV TTCGAA 1 cut(s) 722
PciSI GCTCTTC 1 cut(s) 3447
PctI GAATGC 2 cut(s) 2326, 3044
PdmI GAANNNNTTC 4 cut(s) 216, 690, 2730, 2935
PflMI CCANNNNNTGG 2 cut(s) 437, 1705
PinAI ACCGGT 1 cut(s) 2159
PleI GAGTC 4 cut(s) 1931, 2999, 3379, 4184
PpsI GAGTC 4 cut(s) 1931, 2999, 3379, 4184
PpuMI RGGWCCY 1 cut(s) 3140
Psp124BI GAGCTC 4 cut(s) 1412, 2741, 3250, 3850
Psp5II RGGWCCY 1 cut(s) 3140
Psp6I CCWGG 4 cut(s) 651, 2387, 3142, 3822
PspFI CCCAGC 1 cut(s) 3496
PspGI CCWGG 4 cut(s) 651, 2387, 3142, 3822
PspOMI GGGCCC 1 cut(s) 640
PspPI GGNCC 7 cut(s) 4, 640, 641, 1756, 1823, 3140, 3368
PspPPI RGGWCCY 1 cut(s) 3140
PstI CTGCAG 1 cut(s) 3927
PstNI CAGNNNCTG 3 cut(s) 1705, 3143, 3260
PsuI RGATCY 9 cut(s) 602, 1103, 1360, 1603, 1667, 2899, 3101, 3665, 3826
PvuII CAGCTG 5 cut(s) 794, 1229, 2017, 2548, 3779
RsaI GTAC 7 cut(s) 227, 1138, 1941, 2164, 2296, 2978, 3745
RsaNI GTAC 7 cut(s) 226, 1137, 1940, 2163, 2295, 2977, 3744
RseI CAYNNNNRTG 4 cut(s) 414, 433, 2505, 3677
SacI GAGCTC 4 cut(s) 1412, 2741, 3250, 3850
SapI GCTCTTC 1 cut(s) 3447
Sau96I GGNCC 7 cut(s) 4, 640, 641, 1756, 1823, 3140, 3368
SchI GAGTC 4 cut(s) 1932, 2999, 3379, 4184
ScrFI CCNGG 6 cut(s) 653, 2389, 2817, 3144, 3824, 4112
SexAI ACCWGGT 1 cut(s) 3142
SfcI CTRYAG 5 cut(s) 1368, 3135, 3634, 3830, 3923
SfiI GGCCNNNNNGGCC 1 cut(s) 648
SfuI TTCGAA 1 cut(s) 722
SinI GGWCC 3 cut(s) 4, 3140, 3368
SmiMI CAYNNNNRTG 4 cut(s) 414, 433, 2505, 3677
SmlI CTYRAG 7 cut(s) 56, 580, 704, 746, 1485, 3556, 3617
SmoI CTYRAG 7 cut(s) 56, 580, 704, 746, 1485, 3556, 3617
SsiI CCGC 6 cut(s) 1128, 1196, 1470, 1975, 4164, 4421
SspI AATATT 4 cut(s) 1816, 2536, 2798, 3613
SstI GAGCTC 4 cut(s) 1412, 2741, 3250, 3850
StyD4I CCNGG 6 cut(s) 651, 2387, 2815, 3142, 3822, 4110
StyI CCWWGG 8 cut(s) 21, 606, 1001, 1759, 1779, 3179, 3317, 4448
TaiI ACGT 2 cut(s) 593, 2050
TaqI TCGA 8 cut(s) 196, 722, 1022, 1258, 2140, 2702, 2764, 3159
TatI WGTACW 2 cut(s) 225, 3743
TauI GCSGC 2 cut(s) 1130, 1472
TscAI CASTG 6 cut(s) 117, 408, 1525, 1706, 2417, 3265
TseFI GTSAC 3 cut(s) 140, 1319, 3256
Tsp45I GTSAC 3 cut(s) 140, 1319, 3256
TspGWI ACGGA 3 cut(s) 1322, 4053, 4217
TspRI CASTG 6 cut(s) 117, 408, 1525, 1706, 2417, 3265
Van91I CCANNNNNTGG 2 cut(s) 437, 1705
Vha464I CTTAAG 2 cut(s) 580, 704
VneI GTGCAC 2 cut(s) 2408, 2666
VpaK11BI GGWCC 3 cut(s) 4, 3140, 3368
XapI RAATTY 8 cut(s) 724, 1523, 2517, 2524, 2626, 2727, 4059, 4270
XbaI TCTAGA 1 cut(s) 3098
XceI RCATGY 1 cut(s) 1000
XcmI CCANNNNNNNNNTGG 3 cut(s) 1777, 1786, 2285
XmaJI CCTAGG 1 cut(s) 3179
XmiI GTMKAC 3 cut(s) 843, 2775, 3882
XmnI GAANNNNTTC 4 cut(s) 216, 690, 2730, 2935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.