Rroxscaffold_6G00398080

DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005736
Physical Location & Seq
Reverse (-)
19922430 .. 19933180
10751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00398080.1

Sequence Viewer

Length: 3492 bp
ATGGAAAGGATTGGAAGAGTTTGGGCAAACATTGTAAGAAGAGACATACCAAAGCATCATAGAATGTTTACTACTTTTCACCGCAAGCAACTAATTGATGCCAAGAGGGTCTCAGAGAACTGTCAAAGAGAGGTAAAAATGAAGGTGAGTAGATCACTTAAAGTGATGAGGGGTGCTGCAATTCGCACAAGGAAACTAGCCAGAGACATGCTACTGCTTTGGAAGAGAATAGACAAAGAGATGGCAGAAGTGAGGAAGAAAGAGGAAAAAGAAGCTATTGAAATGCGCAAGCGTCTGGAGGAGGAAAGAGAAGCAAAGAGACAGGAGCAAAGGCTCAATTTTCTCATAAAACAAACCGAACTTTACAGTCACTTCATGCAGAACAAGTTGAGTTCACAGCCATCTGAAGACTTGCCTGTAGGAGACGAAAACCAAGATGACCAAGACGTATCTCCAAGCTCCTCAGATATTGAGAATATTGAAGAAGATTCTGAGGAAGCTGAACTGAAGAAGGAGGCATTAAAAGCTGCTCAAGATGCAGTTTCAAAGCAGAAAAAGTTAACTAGCGCATTTGATGACGAGTGTCTGAGACTGCGTGAAGCCTCTGAACCTGAGGCACCACAGGATGTTGCTGGAGCTAATAACATTGATCTGCATAACCCTTCCACTATGCCTGTTACATCAACCGTTCAGACGCCAGAGTTGTTCAAAGGTTCCCTTAAAGAATACCAGCTAAAAGGCCTCCAGTGGCTGGTTAATTGTTATGAGCAGGGTTTAAATGGCATCCTTGCTGATGAGATGGGCCTGGGTAAGACCATCCAGGCCATGGCATTTTTGGCTCATTTGGCTGAGGAGAAAAATATATGGGGGCCTTTTCTGGTTGTTGCTCCTGCTTCTGTCTTGAACAACTGGGCTGATGAAATTAGTCGTTTCTGCCCCGACTTGAAAACTCTTCCGTATTGGGGTGGCCTTCAAGAGCGTACGGTACTTAGGAAGAAGATCAATGCTAAGAAACTTTACCGTAGGGATGCTGGGTTTCACATTCTCATTACCAGCTACCAGTTGCTAGTGGCTGATGAGAAGTGTTTCCGGCGTGTGAAATGGCAATATATGGTATTGGATGAAGCACAAGCTATCAAAAGTTCTAACAGTATAAGATGGAAGACACTGCTCAGCTTTAATTGTCGAAATCGTTTGCTGCTTACTGGTACACCTATCCAGAATAATATGGCAGAGTTGTGGGCCCTACTACATTTCATTATGCCAACCTTATTTGACAGTCATGAACAATTCAATGAGTGGTTCTCTAAAGGAATTGAAAATCATGCGGAACATGGGGGTACTTTGAACGAGCACCAGCTTAACAGATTGCATTCGATATTGAAGCCATTCATGCTCCGTCGAGTTAAGACAGATGTGATTTCTGAGCTGACCAGGAAAACTGAAGTTACTGTGCACTGCAAGTTGAGTTCTCGGCAACAAGCTTTTTATCAAGCTATCAAAAACAAGATATCTCTTGCTGAGCTGTTTGACAACAATCGTGGGCATCTTAATGAGAAGAAAATTTTGAACTTAATGAATATTGTCATTCAGCTAAGAAAAGTCTGCAATCATCCTGAGTTATTTGAGAGGAATGAAGGAAGCACATATCTTCACTTTGGTGAGATTTCAAATTCTCTTCTTCCTCCGCCATTTGGGGAGTTGGAGGACGTACACTACTCAGGAGGTCAAAATCCTATAACATACGAGGTCCCCAAACTTTTATATCAAGAAATACTCCAGAATTCTGAGACATTTTGCTCTGCAGTTCGGCATGGTGTCTACAGTGAATCTTTTCAGAAACATTTTAACATATTTTCACCGCAAAATGTGTATCGATCAATATTCTTGCAAGAGAACAACTCAGATGAGTTGTCGGTTGGAAGTGGAACATTTGGTTTTACTCATCTGATGGATCTGTCCCCTGCAGAGGTTGCATTTTTAGGAACTGGTTCTTTTATGGAGCGGCTAATGTTTTCCATTATGAGATGGGACCGGCAGTTTTTGGATGGATTGATAGACACACTAATGGAAACCATGGATGATGATTCTGAATGCAGCTTTCTTGAGAGTGGAAAAGTGAGAGCTGTTACACGTATGTTGCTGATGCCTTCAAGGTCTATAACTACCATCTTTCAGAAGAAATTGGCCTCTGGACCTGGTGGTACTCCATTTGAGGGTCTAGTTGTCTCTCATCGGGACAGGCTTCTCTCTAATATTAGGCTTCTCCGTTCAACATACACATTCATCCCCCGAACTAGAGCTCCCCCAGTTAATGCCCATTGCTCAGATCGAAACTTCACTTACAAAATGAGCGAAGAACAACAGTACCCCTGGGTGAAGAGGTTGTTTTCTGGCTTTGCGCGAACATCTGACTACAATGGACCCAGAAAACCTGAGACTCCTCATCATCTAATACAAGAGATTGATTCTGAACTGCCTGTTGCACATTCAGCTCTTCAGTTAACATACAGAATTTTTGGGTCTTGTCCGCCAATGCAAAGCTTCGACCCATCAAAAATGCTCACTGATTCGGGAAAACTTCAGACACTTGATATATTGTTGAAACGCTTGAGGGCAGAAAATCATCGTGTTCTCTTGTTTGCACAGATGACAAAGATGCTGAATATTCTTGAGGACTACATGAACTACAGGAAATATAGATACCTCAGACTCGATGGATCTTCCACCATTATGGATCGTAGAGACATGGTCAGAGACTTCCAGCAACGAAGTGATATTTTTGTTTTCTTGCTGAGTACAAGAGCTGGTGGACTAGGCATTAACTTGACAGCTGCAGATACTGTCATATTTTATGAAAGTGATTGGAATCCGACCCTGGATCTACAGGCAATGGATAGAGCTCATCGGTTGGGCCAGACAAAAGATGTTACTGTCTACCGCCTAATTTGTAAAGAAACGGTTGAGGAGAAGATTCTGCAAAGAGCAAGTCAGAAAAATACTGTGCAGCAGCTTGTAATGATGGGTGGTCATGTTCAGGGTGACCTTTTGGCTCCCGAGGATGTTGTATCTCTACTTCTCGATGATGCCCAGTTGGAGCAGAAATTAAGAGAAGCTCCATCACAGGTTAAGGATAGACAAAAGAAGAAACAGACAAAGGGTATACGGGTAGATGCAGAAGGTGATGCCTCTCTGGAAGATTTAACAAACACTGCTGCATCACAAGGTACTGGAAATGAGGAATCTCCGGATGTTGAAAGATCAAAATCCAATAATAAAAAGAGGAAAACGGGTTCTGACAAGCATACTCCGCGGCCGAAGAATCCTCAAAGTACGGATGAACCAGAGGGCTATGAATTGGAGGATTCCTTACAAAATACTGACCCACAAGGTACAAGACCCAAGAGGCCAAAGAGGTCAAAGAAGAGTGTAAATGAAACTCTTGAACCTGCATTTACAGTGGCATCCCCTGTGGTCCCGAGGCAGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBINO Helicase_C SNF2_N

Protein Analysis

1163

Amino Acids

133.6

Weight (kDa)

8.48

Isoelectric Point (pI)

51.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBINO PF13892 3 - 132 2.3e-45 DNA-binding domain
ResIII PF04851 239 - 406 4.4e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 243 - 541 1.6e-85 SNF2-related domain
Helicase_C PF00271 862 - 972 1.9e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 287
Acc36I ACCTGC 1 cut(s) 3457
AccB1I GGYRCC 1 cut(s) 616
AccB7I CCANNNNNTGG 2 cut(s) 751, 826
AccBSI CCGCTC 1 cut(s) 2005
AccI GTMKAC 3 cut(s) 1821, 2937, 3163
AccII CGCG 2 cut(s) 2404, 3313
AccIII TCCGGA 1 cut(s) 3247
AciI CCGC 9 cut(s) 82, 1328, 1688, 1862, 2005, 2531, 2941, 3311, 3313
AclWI GGATC 4 cut(s) 1962, 2728, 2745, 2889
AcoI YGGCCR 1 cut(s) 3314
AcsI RAATTY 4 cut(s) 1563, 1672, 1783, 2514
AcuI CTGAAG 5 cut(s) 426, 527, 1464, 2483, 2567
AcyI GRCGYC 1 cut(s) 695
AfiI CCNNNNNNNGG 6 cut(s) 751, 826, 962, 1694, 1969, 2216
AflIII ACRYGT 1 cut(s) 2132
AjnI CCWGG 6 cut(s) 804, 819, 1433, 2197, 2372, 2877
AleI CACNNNNGTG 1 cut(s) 1659
AloI GAACNNNNNNTCC 4 cut(s) 2287, 2319, 3277, 3309
Alw21I GWGCWC 4 cut(s) 1356, 1458, 2305, 2905
Alw44I GTGCAC 1 cut(s) 1454
AlwI GGATC 4 cut(s) 1962, 2728, 2745, 2889
AlwNI CAGNNNCTG 2 cut(s) 751, 2843
Ama87I CYCGRG 2 cut(s) 3056, 3478
Aor13HI TCCGGA 1 cut(s) 3247
ApaI GGGCCC 1 cut(s) 1246
ApaLI GTGCAC 1 cut(s) 1454
ApeKI GCWGC 8 cut(s) 176, 527, 1198, 2097, 2834, 3007, 3010, 3215
ApoI RAATTY 4 cut(s) 1563, 1672, 1783, 2514
ArsI GACNNNNNNTTYG 2 cut(s) 2974, 3006
Asp700I GAANNNNTTC 4 cut(s) 1085, 1388, 1833, 1990
AspLEI GCGC 3 cut(s) 288, 569, 2404
AsuHPI GGTGA 7 cut(s) 71, 157, 1673, 1851, 2389, 3053, 3194
AvaI CYCGRG 2 cut(s) 3056, 3478
AvaII GGWCC 5 cut(s) 1750, 2032, 2195, 2423, 3475
AxyI CCTNAGG 1 cut(s) 612
BaeGI GKGCMC 2 cut(s) 1246, 1458
BaeI ACNNNNGTAYC 2 cut(s) 2351, 2384
BanI GGYRCC 1 cut(s) 616
BanII GRGCYC 3 cut(s) 1246, 2305, 2905
BarI GAAGNNNNNNTAC 2 cut(s) 3060, 3092
BbsI GAAGAC 2 cut(s) 414, 1169
Bbv12I GWGCWC 4 cut(s) 1356, 1458, 2305, 2905
BbvCI CCTCAGC 1 cut(s) 849
BbvI GCAGC 8 cut(s) 163, 514, 1185, 2109, 2821, 3019, 3022, 3202
BciT130I CCWGG 6 cut(s) 806, 821, 1435, 2199, 2374, 2879
BfaI CTAG 6 cut(s) 197, 564, 1067, 2222, 2298, 2816
BfmI CTRYAG 7 cut(s) 417, 1803, 1822, 1965, 2689, 2835, 2885
BfuAI ACCTGC 1 cut(s) 3457
BlpI GCTNAGC 2 cut(s) 1172, 1521
Bme1390I CCNGG 6 cut(s) 806, 821, 1435, 2199, 2374, 2879
Bme18I GGWCC 5 cut(s) 1750, 2032, 2195, 2423, 3475
BmeT110I CYCGRG 2 cut(s) 3056, 3478
BmiI GGNNCC 9 cut(s) 618, 715, 870, 1244, 1752, 2033, 2425, 3054, 3477
BmrFI CCNGG 6 cut(s) 806, 821, 1435, 2199, 2374, 2879
BmrI ACTGGG 3 cut(s) 919, 2303, 3085
BmuI ACTGGG 3 cut(s) 919, 2303, 3085
BoxI GACNNNNGTC 1 cut(s) 582
BpiI GAAGAC 2 cut(s) 414, 1169
BplI GAGNNNNNCTC 4 cut(s) 1289, 1321, 1886, 1918
BpmI CTGGAG 4 cut(s) 317, 654, 728, 1763
Bpu10I CCTNAGC 1 cut(s) 849
Bpu1102I GCTNAGC 2 cut(s) 1172, 1521
BpuEI CTTGAG 4 cut(s) 516, 2126, 2632, 2693
Bsa29I ATCGAT 1 cut(s) 1876
BsaAI YACGTR 1 cut(s) 2135
BsaBI GATNNNNATC 2 cut(s) 2868, 3265
BsaHI GRCGYC 1 cut(s) 695
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 9 cut(s) 805, 825, 2076, 2372, 2373, 2877, 3057, 3311, 3479
BsaWI WCCGGW 1 cut(s) 3247
BsaXI ACNNNNNCTCC 2 cut(s) 2287, 2317
Bsc4I CCNNNNNNNGG 6 cut(s) 751, 826, 962, 1694, 1969, 2216
Bse118I RCCGGY 1 cut(s) 2034
Bse1I ACTGG 8 cut(s) 745, 914, 1060, 1210, 1993, 2309, 3091, 3235
Bse21I CCTNAGG 1 cut(s) 612
Bse3DI GCAATG 2 cut(s) 2320, 2898
Bse8I GATNNNNATC 2 cut(s) 2868, 3265
BseAI TCCGGA 1 cut(s) 3247
BseBI CCWGG 6 cut(s) 806, 821, 1435, 2199, 2374, 2879
BseCI ATCGAT 1 cut(s) 1876
BseDI CCNNGG 9 cut(s) 805, 825, 2076, 2372, 2373, 2877, 3057, 3311, 3479
BseJI GATNNNNATC 2 cut(s) 2868, 3265
BseLI CCNNNNNNNGG 6 cut(s) 751, 826, 962, 1694, 1969, 2216
BseMI GCAATG 2 cut(s) 2320, 2898
BseNI ACTGG 8 cut(s) 745, 914, 1060, 1210, 1993, 2309, 3091, 3235
BseRI GAGGAG 5 cut(s) 314, 451, 866, 2433, 2981
BseSI GKGCMC 2 cut(s) 1246, 1458
BseX3I CGGCCG 1 cut(s) 3314
BseXI GCAGC 8 cut(s) 163, 514, 1185, 2109, 2821, 3019, 3022, 3202
BseYI CCCAGC 1 cut(s) 1031
BsgI GTGCAG 1 cut(s) 3026
Bsh1236I CGCG 2 cut(s) 2404, 3313
Bsh1285I CGRYCG 1 cut(s) 3317
BshNI GGYRCC 1 cut(s) 616
BshVI ATCGAT 1 cut(s) 1876
BsiEI CGRYCG 1 cut(s) 3317
BsiHKAI GWGCWC 4 cut(s) 1356, 1458, 2305, 2905
BsiHKCI CYCGRG 2 cut(s) 3056, 3478
BsiSI CCGG 3 cut(s) 1090, 2035, 3248
BsiWI CGTACG 1 cut(s) 980
BslFI GGGAC 5 cut(s) 1736, 1945, 2045, 2252, 3461
BslI CCNNNNNNNGG 6 cut(s) 751, 826, 962, 1694, 1969, 2216
BsmBI CGTCTC 1 cut(s) 417
BsmFI GGGAC 5 cut(s) 1736, 1945, 2045, 2252, 3461
BsmI GAATGC 2 cut(s) 1372, 2099
Bso31I GGTCTC 1 cut(s) 115
BsoBI CYCGRG 2 cut(s) 3056, 3478
Bsp120I GGGCCC 1 cut(s) 1242
Bsp1286I GDGCHC 5 cut(s) 1246, 1356, 1458, 2305, 2905
Bsp13I TCCGGA 1 cut(s) 3247
Bsp1720I GCTNAGC 2 cut(s) 1172, 1521
Bsp19I CCATGG 2 cut(s) 825, 2076
BspACI CCGC 9 cut(s) 82, 1328, 1688, 1862, 2005, 2531, 2941, 3311, 3313
BspDI ATCGAT 1 cut(s) 1876
BspEI TCCGGA 1 cut(s) 3247
BspFNI CGCG 2 cut(s) 2404, 3313
BspHI TCATGA 1 cut(s) 1282
BspLI GGNNCC 9 cut(s) 618, 715, 870, 1244, 1752, 2033, 2425, 3054, 3477
BspMAI CTGCAG 3 cut(s) 1807, 1969, 2839
BspMI ACCTGC 1 cut(s) 3457
BspPI GGATC 4 cut(s) 1962, 2728, 2745, 2889
BspQI GCTCTTC 1 cut(s) 2502
BspT107I GGYRCC 1 cut(s) 616
BspTNI GGTCTC 1 cut(s) 115
BsrBI CCGCTC 1 cut(s) 2005
BsrDI GCAATG 2 cut(s) 2320, 2898
BsrFI RCCGGY 1 cut(s) 2034
BsrI ACTGG 8 cut(s) 745, 914, 1060, 1210, 1993, 2309, 3091, 3235
BssAI RCCGGY 1 cut(s) 2034
BssECI CCNNGG 9 cut(s) 805, 825, 2076, 2372, 2373, 2877, 3057, 3311, 3479
BssNAI GTATAC 1 cut(s) 3164
BssNI GRCGYC 1 cut(s) 695
BssT1I CCWWGG 2 cut(s) 825, 2076
Bst1107I GTATAC 1 cut(s) 3164
Bst2UI CCWGG 6 cut(s) 806, 821, 1435, 2199, 2374, 2879
Bst6I CTCTTC 8 cut(s) 10, 34, 218, 957, 1683, 2375, 2502, 3419
BstACI GRCGYC 1 cut(s) 695
BstAPI GCANNNNNTGC 1 cut(s) 1973
BstBAI YACGTR 1 cut(s) 2135
BstC8I GCNNGC 2 cut(s) 86, 290
BstDSI CCRYGG 3 cut(s) 825, 2076, 3311
BstEII GGTNACC 1 cut(s) 3041
BstFNI CGCG 2 cut(s) 2404, 3313
BstHHI GCGC 3 cut(s) 288, 569, 2404
BstMCI CGRYCG 1 cut(s) 3317
BstMWI GCNNNNNNNGC 8 cut(s) 524, 536, 836, 845, 1393, 1973, 2492, 3310
BstNI CCWGG 6 cut(s) 806, 821, 1435, 2199, 2374, 2879
BstNSI RCATGY 1 cut(s) 211
BstPAI GACNNNNGTC 1 cut(s) 582
BstPI GGTNACC 1 cut(s) 3041
BstSCI CCNGG 6 cut(s) 804, 819, 1433, 2197, 2372, 2877
BstSFI CTRYAG 7 cut(s) 417, 1803, 1822, 1965, 2689, 2835, 2885
BstSLI GKGCMC 2 cut(s) 1246, 1458
BstUI CGCG 2 cut(s) 2404, 3313
BstV1I GCAGC 8 cut(s) 163, 514, 1185, 2109, 2821, 3019, 3022, 3202
BstV2I GAAGAC 2 cut(s) 414, 1169
BstX2I RGATCY 3 cut(s) 1954, 2720, 2881
BstXI CCANNNNNNTGG 1 cut(s) 2734
BstYI RGATCY 3 cut(s) 1954, 2720, 2881
BstZ17I GTATAC 1 cut(s) 3164
BstZI CGGCCG 1 cut(s) 3314
Bsu15I ATCGAT 1 cut(s) 1876
Bsu36I CCTNAGG 1 cut(s) 612
BsuTUI ATCGAT 1 cut(s) 1876
BtgI CCRYGG 3 cut(s) 825, 2076, 3311
BtsI GCAGTG 3 cut(s) 1166, 1456, 3210
BtsIMutI CAGTG 7 cut(s) 752, 1166, 1456, 1831, 2565, 3210, 3465
BveI ACCTGC 1 cut(s) 3457
Cac8I GCNNGC 2 cut(s) 86, 290
CaiI CAGNNNCTG 2 cut(s) 751, 2843
CciI TCATGA 1 cut(s) 1282
CfoI GCGC 3 cut(s) 288, 569, 2404
Cfr10I RCCGGY 1 cut(s) 2034
Cfr42I CCGCGG 1 cut(s) 3314
ClaI ATCGAT 1 cut(s) 1876
CseI GACGC 2 cut(s) 281, 703
CsiI ACCWGGT 1 cut(s) 2197
CspCI CAANNNNNGTGG 2 cut(s) 1522, 1557
DraI TTTAAA 1 cut(s) 777
EaeI YGGCCR 1 cut(s) 3314
EagI CGGCCG 1 cut(s) 3314
Eam1104I CTCTTC 8 cut(s) 10, 34, 218, 957, 1683, 2375, 2502, 3419
EarI CTCTTC 8 cut(s) 10, 34, 218, 957, 1683, 2375, 2502, 3419
EciI GGCGGA 2 cut(s) 1677, 2520
Ecl136II GAGCTC 2 cut(s) 2303, 2903
EclXI CGGCCG 1 cut(s) 3314
Eco130I CCWWGG 2 cut(s) 825, 2076
Eco147I AGGCCT 1 cut(s) 741
Eco24I GRGCYC 3 cut(s) 1246, 2305, 2905
Eco31I GGTCTC 1 cut(s) 115
Eco32I GATATC 1 cut(s) 1512
Eco47I GGWCC 5 cut(s) 1750, 2032, 2195, 2423, 3475
Eco52I CGGCCG 1 cut(s) 3314
Eco53kI GAGCTC 2 cut(s) 2303, 2903
Eco57I CTGAAG 5 cut(s) 426, 527, 1464, 2483, 2567
Eco81I CCTNAGG 1 cut(s) 612
Eco88I CYCGRG 2 cut(s) 3056, 3478
Eco91I GGTNACC 1 cut(s) 3041
EcoICRI GAGCTC 2 cut(s) 2303, 2903
EcoO109I RGGNCCY 3 cut(s) 869, 1243, 1750
EcoO65I GGTNACC 1 cut(s) 3041
EcoRI GAATTC 1 cut(s) 1783
EcoRII CCWGG 6 cut(s) 804, 819, 1433, 2197, 2372, 2877
EcoRV GATATC 1 cut(s) 1512
EcoT14I CCWWGG 2 cut(s) 825, 2076
EcoT38I GRGCYC 3 cut(s) 1246, 2305, 2905
ErhI CCWWGG 2 cut(s) 825, 2076
Esp3I CGTCTC 1 cut(s) 417
FalI AAGNNNNNCTT 2 cut(s) 702, 734
FaqI GGGAC 5 cut(s) 1736, 1945, 2045, 2252, 3461
FblI GTMKAC 3 cut(s) 1821, 2937, 3163
FriOI GRGCYC 3 cut(s) 1246, 2305, 2905
FspBI CTAG 6 cut(s) 197, 564, 1067, 2222, 2298, 2816
FspI TGCGCA 1 cut(s) 287
GlaI GCGC 3 cut(s) 287, 568, 2403
GsaI CCCAGC 1 cut(s) 1035
GsuI CTGGAG 4 cut(s) 317, 654, 728, 1763
HapII CCGG 3 cut(s) 1090, 2035, 3248
HgaI GACGC 2 cut(s) 281, 703
HhaI GCGC 3 cut(s) 288, 569, 2404
Hin1I GRCGYC 1 cut(s) 695
Hin6I GCGC 3 cut(s) 286, 567, 2402
HinP1I GCGC 3 cut(s) 286, 567, 2402
HincII GTYRAC 2 cut(s) 561, 2505
HindII GTYRAC 2 cut(s) 561, 2505
HindIII AAGCTT 2 cut(s) 1482, 2542
HpaI GTTAAC 2 cut(s) 561, 2505
HpaII CCGG 3 cut(s) 1090, 2035, 3248
HphI GGTGA 7 cut(s) 71, 157, 1673, 1851, 2389, 3053, 3194
Hpy99I CGWCG 1 cut(s) 1404
HpyAV CCTTC 7 cut(s) 136, 505, 672, 980, 1631, 2160, 3173
HpyCH4IV ACGT 3 cut(s) 447, 1710, 2134
HpyF10VI GCNNNNNNNGC 8 cut(s) 524, 536, 836, 845, 1393, 1973, 2492, 3310
HpySE526I ACGT 3 cut(s) 447, 1710, 2134
Hsp92I GRCGYC 1 cut(s) 695
HspAI GCGC 3 cut(s) 286, 567, 2402
Kpn2I TCCGGA 1 cut(s) 3247
KspAI GTTAAC 2 cut(s) 561, 2505
KspI CCGCGG 1 cut(s) 3314
LguI GCTCTTC 1 cut(s) 2502
Lsp1109I GCAGC 8 cut(s) 163, 514, 1185, 2109, 2821, 3019, 3022, 3202
MabI ACCWGGT 1 cut(s) 2197
MaeI CTAG 6 cut(s) 197, 564, 1067, 2222, 2298, 2816
MaeII ACGT 3 cut(s) 447, 1710, 2134
MaeIII GTNAC 6 cut(s) 368, 676, 1447, 2128, 2929, 3041
MbiI CCGCTC 1 cut(s) 2005
MflI RGATCY 3 cut(s) 1954, 2720, 2881
MhlI GDGCHC 5 cut(s) 1246, 1356, 1458, 2305, 2905
MlyI GAGTC 2 cut(s) 2434, 2706
MmeI TCCRAC 4 cut(s) 1683, 1900, 2897, 3075
MroI TCCGGA 1 cut(s) 3247
MroXI GAANNNNTTC 4 cut(s) 1085, 1388, 1833, 1990
MslI CAYNNNNRTG 4 cut(s) 1551, 1659, 2066, 2732
MspA1I CMGCKG 2 cut(s) 2834, 3313
MspI CCGG 3 cut(s) 1090, 2035, 3248
MspR9I CCNGG 6 cut(s) 806, 821, 1435, 2199, 2374, 2879
Mva1269I GAATGC 2 cut(s) 1372, 2099
MvaI CCWGG 6 cut(s) 806, 821, 1435, 2199, 2374, 2879
MvnI CGCG 2 cut(s) 2404, 3313
MwoI GCNNNNNNNGC 8 cut(s) 524, 536, 836, 845, 1393, 1973, 2492, 3310
NcoI CCATGG 2 cut(s) 825, 2076
NlaIV GGNNCC 9 cut(s) 618, 715, 870, 1244, 1752, 2033, 2425, 3054, 3477
NmeAIII GCCGAG 1 cut(s) 1453
NmuCI GTSAC 2 cut(s) 368, 3041
NsbI TGCGCA 1 cut(s) 287
NspI RCATGY 1 cut(s) 211
OliI CACNNNNGTG 1 cut(s) 1659
PagI TCATGA 1 cut(s) 1282
PasI CCCWGGG 1 cut(s) 2373
PceI AGGCCT 1 cut(s) 741
PciSI GCTCTTC 1 cut(s) 2502
PctI GAATGC 2 cut(s) 1372, 2099
PdmI GAANNNNTTC 4 cut(s) 1085, 1388, 1833, 1990
Pfl23II CGTACG 1 cut(s) 980
PflFI GACNNNGTC 1 cut(s) 2750
PflMI CCANNNNNTGG 2 cut(s) 751, 826
PleI GAGTC 2 cut(s) 2434, 2706
PpsI GAGTC 2 cut(s) 2434, 2706
Ppu21I YACGTR 1 cut(s) 2135
PpuMI RGGWCCY 1 cut(s) 1750
PshAI GACNNNNGTC 1 cut(s) 582
Psp124BI GAGCTC 2 cut(s) 2305, 2905
Psp5II RGGWCCY 1 cut(s) 1750
Psp6I CCWGG 6 cut(s) 804, 819, 1433, 2197, 2372, 2877
PspEI GGTNACC 1 cut(s) 3041
PspFI CCCAGC 1 cut(s) 1031
PspGI CCWGG 6 cut(s) 804, 819, 1433, 2197, 2372, 2877
PspLI CGTACG 1 cut(s) 980
PspN4I GGNNCC 9 cut(s) 618, 715, 870, 1244, 1752, 2033, 2425, 3054, 3477
PspOMI GGGCCC 1 cut(s) 1242
PspPPI RGGWCCY 1 cut(s) 1750
PstI CTGCAG 3 cut(s) 1807, 1969, 2839
PstNI CAGNNNCTG 2 cut(s) 751, 2843
PsuI RGATCY 3 cut(s) 1954, 2720, 2881
PsyI GACNNNGTC 1 cut(s) 2750
PvuII CAGCTG 1 cut(s) 2834
RseI CAYNNNNRTG 4 cut(s) 1551, 1659, 2066, 2732
SacI GAGCTC 2 cut(s) 2305, 2905
SacII CCGCGG 1 cut(s) 3314
SapI GCTCTTC 1 cut(s) 2502
SchI GAGTC 2 cut(s) 2434, 2706
ScrFI CCNGG 6 cut(s) 806, 821, 1435, 2199, 2374, 2879
SduI GDGCHC 5 cut(s) 1246, 1356, 1458, 2305, 2905
SexAI ACCWGGT 1 cut(s) 2197
SfcI CTRYAG 7 cut(s) 417, 1803, 1822, 1965, 2689, 2835, 2885
Sfr303I CCGCGG 1 cut(s) 3314
SgrBI CCGCGG 1 cut(s) 3314
SinI GGWCC 5 cut(s) 1750, 2032, 2195, 2423, 3475
SmiMI CAYNNNNRTG 4 cut(s) 1551, 1659, 2066, 2732
SmlI CTYRAG 4 cut(s) 531, 2105, 2611, 2672
SmoI CTYRAG 4 cut(s) 531, 2105, 2611, 2672
SseBI AGGCCT 1 cut(s) 741
SsiI CCGC 9 cut(s) 82, 1328, 1688, 1862, 2005, 2531, 2941, 3311, 3313
SspI AATATT 5 cut(s) 478, 1582, 1884, 2257, 2668
SspMI CTAG 6 cut(s) 197, 564, 1067, 2222, 2298, 2816
SstI GAGCTC 2 cut(s) 2305, 2905
StuI AGGCCT 1 cut(s) 741
StyD4I CCNGG 6 cut(s) 804, 819, 1433, 2197, 2372, 2877
StyI CCWWGG 2 cut(s) 825, 2076
TaiI ACGT 3 cut(s) 450, 1713, 2137
TaqI TCGA 8 cut(s) 1186, 1376, 1402, 1876, 2332, 2547, 2715, 3081
TatI WGTACW 1 cut(s) 2798
TauI GCSGC 2 cut(s) 2008, 3316
TscAI CASTG 7 cut(s) 752, 1173, 1463, 1831, 2572, 3217, 3465
TseFI GTSAC 2 cut(s) 368, 3041
TseI GCWGC 8 cut(s) 176, 527, 1198, 2097, 2834, 3007, 3010, 3215
Tsp45I GTSAC 2 cut(s) 368, 3041
TspGWI ACGGA 4 cut(s) 945, 1388, 2258, 3350
TspRI CASTG 7 cut(s) 752, 1173, 1463, 1831, 2572, 3217, 3465
Tth111I GACNNNGTC 1 cut(s) 2750
Van91I CCANNNNNTGG 2 cut(s) 751, 826
VneI GTGCAC 1 cut(s) 1454
VpaK11BI GGWCC 5 cut(s) 1750, 2032, 2195, 2423, 3475
XapI RAATTY 4 cut(s) 1563, 1672, 1783, 2514
XceI RCATGY 1 cut(s) 211
XcmI CCANNNNNNNNNTGG 2 cut(s) 823, 832
XmiI GTMKAC 3 cut(s) 1821, 2937, 3163
XmnI GAANNNNTTC 4 cut(s) 1085, 1388, 1833, 1990
XspI CTAG 6 cut(s) 197, 564, 1067, 2222, 2298, 2816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.