RLG00000023256

DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005736
Physical Location & Seq
Reverse (-)
20680476 .. 20691506
11031 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023256

Sequence Viewer

Length: 4533 bp
ATGGACCACAGGCGCCAATCCAAGGATTCTCTCTCCTCCTCCTACTCCAACCTCTTCAATCTTGAGCCTTTGATGAACTTTCAACTTCCACAGCCGGATGATGATTTTGATTATTACGGCAATAGTAGTCAGGATGAGAGCAGAGGTAGCCAAGGTGGAGCTGCTGGTAATGGGATTATGTCCGACCGCGAACTGAATTCAGTAAAAAAAAGGAGGCGGTCTCAAAACAGTGATTACGAGGAGGATGACAGTTATTATGGGACGCACATTACGGAGGACAAGTATAGATCAATGCTTGGAGAACACATTCAGAAGTACAAAAGGAGGTTCAAGGATTCCTCGTCAAGTCCTGCACCAATCCAGATGGGGATTCCGGTTTCAAAGGGTAATAAGGGTTCGAAATCGAGGAAACTGGCGAATGAGAACCGAGGAGGGTTTTATGAAATGGAAACCACGTCTGAGTGGCTCAATGATACTATTCCTCAGAAACCAGGGAACTACCAGGATGCTGATTTTGCGCCACAAAGTGCAGCTGATAGAGTAATATATGAACCTCCTTATCTGGATATTGGAGATGGTTTCACATACAAGATTCCACCAATTTATGATAAACTGGTGACATCTTTACACTTGCCAAGCTTTTCTGATTTTCGGGTGGAGGAAGTTTACTTGAAAGGTACATTGGATTTGGGATCCTTAGCAGAAATGATGGGTAGTGATAAAAGGTTTGGTCCAAAAAATCGGGCAGGAATGGGGGAGCCCTATCCCCAGTATGAGTCCCTTCAGGCAAGATTGAAGGCCTTGTCCACTTCTCCCTCTGCTCAAAAGTTCAGCCTCAAAGTTTCTGATATTGGGTTGAATTCCTCCATCCCAGAGGGGGCAGCTGGAAGTATAAAGAGGTCTATTTTGTCTGAGGGTGGTGTCTTGCAAGTCTACTATGTGAAGGTTTTGGAGAAAGGAGACACATATGAGATAATTGAACGAAGCCTACCCAAGAAGCAAAAGGTACAGAAAGATCCTTCTCAGATTGAGAAGGAGGAAATGGAAAGGATTGGAAGAGTTTGGGTAAACATTGTAAGAAGAGACATACCAAAGCATCATAGAATGTTTACTACTTTTCACCGCAAGCAACTAATTGATGCCAAGAGGGTCTCAGAGAACTGTCAAAGAGAGGTAAAAATGAAGGTGAGTAGATCACTTAAAGTGATGAGGGGTGCTGCAATTCGCACAAGGAAACTAGCCAGAGACATGCTACTGCTTTGGAAGAGAATAGACAAAGAGATGGCAGAAGTGAGGAAGAAAGAGGAAAAAGAAGCTATTGAAATGCGCAAGCGTCTGGAGGAGGAAAGAGAAGCAAAGAGACAGGAGCAAAGGCTCAATTTTCTCATAAAACAAACCGAACTTTACAGTCACTTTATGCAGAACAAGTTGAGTTCACAGCCATCTGAAGACTTGCCTGTAGGAGATGAAAACCAAGATGACCAAGATGTATCTCCAAGCTCCTCAGATATTGAGAATATTGAAGAAGATTCTGAGGAAGCTGAACTGAAGAAGGAGGCATTAAAAGCTGCTCAAGATGCAGTTTCAAAGCAGAAAAAGTTAACTAGTGCATTTGATGACGAGTGTCTGAGACTGCGTGAAGCTGCTGAACCTGAGGCCCCACAGGATGTTGCTGGAGCTAATAACATTGATCTGCATAACCCTTCCACTATGCCTGTTACATCAACCGTTCAGACACCAGAGTTGTTCAAAGGTTCCCTTAAAGAATACCAGCTAAAAGGCCTCCAGTGGCTGGTTAATTGTTATGAGCAGGGTTTAAATGGCATCCTTGCTGATGAGATGGGCCTGGGTAAGACCATCCAGGCCATGGCATTTTTGGCTCATTTGGCTGAGGAGAAAAATATATGGGGGCCTTTTCTGGTTGTTGCTCCTGCTTCTGTCTTGAACAACTGGGCTGATGAAATTAGTCGTTTCTGCCCCGACTTGAAAACTCTTCCATATTGGGGTGGCCTTCAAGAGCGTATGGTACTTAGGAAGAAAATCAACGCTAAGAAACTTTACCGTAGGGATGCTGGGTTTCACATTCTCATTACCAGCTACCAGTTGCTAGTGGGTGATGAGAAGTGTTTCCGGCGTGTGAAATGGCAATATATGGTATTGGATGAAGCACAAGCTATCAAAAGTTCTAACAGTATAAGATGGAAGACACTGCTCAGCTTTAATTGTCGAAATCGTTTGCTGCTTACTGGTACACCTATCCAGAATAATATGGCAGAGTTGTGGGCCCTACTACATTTCATTATGCCAACCTTATTTGACAGTCATGAACAATTCAATGAGTGGTTCTCTAAAGGAATTGAAAATCATGCGGAACATGGGGGTACTTTGAACGAGCACCAGCTTAACAGATTGCATTCGATATTGAAGCCATTCATGCTCCGTCGAGTTAAGACAGACGTGATTTCTGAGCTGACCAGGAAAACTGAAGTTACTGTGCACTGCAAGTTGAGTTCTCGGCAACAAGCTTTTTATCAAGCTATCAAAAACAAGATATCTCTTGCTGAGCTGTTTGACAACAATCGTGGGCATCTTAATGAGAAGAAAATTTTGAACTTAATGAATATTGTCATTCAGCTAAGAAAAGTCTGCAACCATCCTGAGTTATTTGAGAGGAATGAAGGAAGCACATATCTTCACTTTGGTGAGATTTCAAATTCTCTTCTACCTCCGCCATTTGGGGAGTTGGAGGATGTACACTACTCGGGAGGTCAAAATCCTATAACATATGAGGTCCCCAAACTTTTATATCAAGAAATACTCCAGAATTCTGAAACGTTTTGCTCTGCAGTTCGGCATGGTGTCTACAATGAATCTTTTCAGAAACATTTTAACATATTTTCACCGCAAAATGTGTACCGATCAATATTCTTGCAAGAGAATGACTCAGATGAGTTGTCGGTTGGAAGTGGAACGTTTGGTTTTACTCATCTGATGGATCTGTCCCCTGCAGAGGTTGCATTTTTAGGAACTGGTTCTTTTATGGAGCGGCTAATGTTTTCCATTATGAGATGGGACCGGCAGTTTTTGGATGGATTGATAGACACACTAATGGAAACCATGGATGATGATTCTGAATGCAGCTTTCTTGAGAGTGGAAAAGTGAGAGCTGTTACACGTATGTTGCTGATGCCTTCAAGATCTATAACTACCATCTTTCAGAAGAAATTGGCTACTGGACCTGGTGGTACTCCATTTGTGGGTCTAGTTGTCTCTCATCAGGACAGGCTTCTCTCTAATATTAGGCTTCTCCGTTCGACATACACATTCATCCCCCGAACTAGAGCTCCCCCAGTTAATGCCCATTGCTCAGATCGAAACTTCACTTACAGAATGAGTGAAGAACAACAGCACCCCTGGGTGAAGAGGTTGTTTTCTGGCTTTGCGCGAACATCTGACTACAATGGACCCAGAAAACCTGAGACTCCTCATCATCTAATACAAGAGATTGATTCTGAACTGCCTGTTTCACATTCAGCTCTTCAGTTAACATACAGAATTTTTGGGTCTTGTCCTCCGATGCAAAGCTTTGACCCGGCAAAAATGCTCACTGATTCGGGGAAACTTCAGACACTTGATATATTGTTGAAACGCTTGAGGGCAGAAAATCATCGTGTTCTTTTGTTTGCACAGATGACAAAGATGCTGAATATTCTTGAGGACTACATGAACTACAGGAAATATAGATACCTCAGACTCGATGGATCTTCCACGATTATGGATCGTAGAGACATGGTCAGAGACTTCCAGCAACGAAGTGATATTTTTGTTTTCTTGCTGAGTACAAGAGCTGGTGGACTAGGCATTAACTTGACAGCTGCAGATACTGTTATATTTTATGAAAGTGATTGGAATCCGACCCTGGATCTACAGGCAATGGATAGAGCTCATCGGTTGGGCCAGACAAAAGATGTTACTGTCTACCGCCTAATTTGTAAAGAAACGGTTGAGGAGAAGATTCTGCAAAGAGCAAGTCAGAAAAATACTGTGCAGCAGCTTGTAATGATGGGTGGTCATGTTCAGGGTGACCTTTTGGCTCCCGAGGATGTTGTATCTCTACTTCTCGACGATGCCCAGTTGGAGCAGAAATTAAGAGAAGCTCCATCACATGTTAAGGATAGACAAAAGAAGAAACAGACAAAGGGTATACGGGTAGATGCAGAAGGTGATGCCTCTTTGGAAGATTTAACAAACACTGCTGCATCACAAGGTACTGGAAATGAGGAATCTCCGGATGTCGAAAGATCAAAATCCAATAATAAAAAGAGGAAAACAGTACCTGACAAGCATACTCCGCGGCCGAAGAATCCTCAAAGTATGGATGAACTAGAGGCCTATGAATTGGAGGATTCCTTACAAAATACTGATCCACAAGGTACAAGACCCAAGAGGCCAAAGAGGTCAAAGAAGAGTGTCAATGAAACTCTTGAACCTGCATTTACAGCGGCATCCCCTGTGGTCCCGAGGCAGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBINO Helicase_C SNF2_N

Protein Analysis

1511

Amino Acids

172.7

Weight (kDa)

7.8

Isoelectric Point (pI)

53.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBINO PF13892 349 - 479 1.1e-44 DNA-binding domain
ResIII PF04851 586 - 753 1.1e-07 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 590 - 888 4.1e-85 SNF2-related domain
Helicase_C PF00271 1209 - 1319 2.7e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1328
Acc36I ACCTGC 1 cut(s) 4498
AccB1I GGYRCC 1 cut(s) 12
AccB7I CCANNNNNTGG 2 cut(s) 1792, 1867
AccBSI CCGCTC 1 cut(s) 3046
AccI GTMKAC 4 cut(s) 933, 2862, 3978, 4204
AccII CGCG 3 cut(s) 189, 3445, 4354
AccIII TCCGGA 1 cut(s) 4288
AclI AACGTT 2 cut(s) 2834, 2972
AclWI GGATC 8 cut(s) 687, 700, 1010, 3003, 3769, 3786, 3930, 4418
AcoI YGGCCR 1 cut(s) 4355
AcsI RAATTY 6 cut(s) 196, 859, 2604, 2713, 2824, 3555
AcuI CTGAAG 6 cut(s) 767, 1467, 1568, 2505, 3524, 3608
AcyI GRCGYC 1 cut(s) 13
AdeI CACNNNGTG 1 cut(s) 527
AfiI CCNNNNNNNGG 8 cut(s) 22, 877, 1792, 1867, 2003, 2735, 3010, 3257
AflIII ACRYGT 2 cut(s) 3173, 4165
AhlI ACTAGT 1 cut(s) 1604
AjiI CACGTC 2 cut(s) 456, 2458
AjnI CCWGG 8 cut(s) 490, 501, 1845, 1860, 2474, 3238, 3413, 3918
AjuI GAANNNNNNNTTGG 2 cut(s) 665, 697
AleI CACNNNNGTG 1 cut(s) 2700
AloI GAACNNNNNNTCC 2 cut(s) 3328, 3360
Alw21I GWGCWC 4 cut(s) 2397, 2499, 3346, 3946
Alw44I GTGCAC 1 cut(s) 2495
AlwI GGATC 8 cut(s) 687, 700, 1010, 3003, 3769, 3786, 3930, 4418
AlwNI CAGNNNCTG 3 cut(s) 1792, 3884, 4337
Ama87I CYCGRG 3 cut(s) 2761, 4097, 4519
Aor13HI TCCGGA 1 cut(s) 4288
ApaI GGGCCC 1 cut(s) 2287
ApaLI GTGCAC 1 cut(s) 2495
ApoI RAATTY 6 cut(s) 196, 859, 2604, 2713, 2824, 3555
ArsI GACNNNNNNTTYG 2 cut(s) 4015, 4047
Asp700I GAANNNNTTC 5 cut(s) 306, 2126, 2429, 2874, 3031
AspLEI GCGC 4 cut(s) 15, 520, 1329, 3445
AsuC2I CCSGG 1 cut(s) 3593
AsuHPI GGTGA 9 cut(s) 628, 1112, 1198, 2126, 2714, 2892, 3430, 4094, 4235
AsuII TTCGAA 1 cut(s) 398
AvaI CYCGRG 3 cut(s) 2761, 4097, 4519
AvaII GGWCC 7 cut(s) 4, 731, 2791, 3073, 3236, 3464, 4516
AxyI CCTNAGG 1 cut(s) 1653
BaeGI GKGCMC 2 cut(s) 2287, 2499
BamHI GGATCC 1 cut(s) 692
BanI GGYRCC 1 cut(s) 12
BanII GRGCYC 4 cut(s) 762, 2287, 3346, 3946
BarI GAAGNNNNNNTAC 2 cut(s) 4101, 4133
BbsI GAAGAC 2 cut(s) 1455, 2210
Bbv12I GWGCWC 4 cut(s) 2397, 2499, 3346, 3946
BbvCI CCTCAGC 1 cut(s) 1890
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 8 cut(s) 492, 503, 1847, 1862, 2476, 3240, 3415, 3920
BcnI CCSGG 1 cut(s) 3593
BcuI ACTAGT 1 cut(s) 1604
BfaI CTAG 7 cut(s) 1238, 1605, 2108, 3263, 3339, 3857, 4385
BfmI CTRYAG 6 cut(s) 1458, 2844, 3006, 3730, 3876, 3926
BfoI RGCGCY 1 cut(s) 16
BfuAI ACCTGC 1 cut(s) 4498
BglII AGATCT 1 cut(s) 3197
BlpI GCTNAGC 2 cut(s) 2213, 2562
Bme1390I CCNGG 9 cut(s) 492, 503, 1847, 1862, 2476, 3240, 3415, 3593, 3920
Bme18I GGWCC 7 cut(s) 4, 731, 2791, 3073, 3236, 3464, 4516
BmeT110I CYCGRG 3 cut(s) 2761, 4097, 4519
BmgBI CACGTC 2 cut(s) 456, 2458
BmrFI CCNGG 9 cut(s) 492, 503, 1847, 1862, 2476, 3240, 3415, 3593, 3920
BmrI ACTGGG 4 cut(s) 763, 1960, 3344, 4126
BmuI ACTGGG 4 cut(s) 763, 1960, 3344, 4126
BoxI GACNNNNGTC 1 cut(s) 1623
BpiI GAAGAC 2 cut(s) 1455, 2210
BplI GAGNNNNNCTC 6 cut(s) 205, 237, 2330, 2362, 2927, 2959
BpmI CTGGAG 4 cut(s) 1358, 1695, 1769, 2804
Bpu10I CCTNAGC 2 cut(s) 697, 1890
Bpu1102I GCTNAGC 2 cut(s) 2213, 2562
Bpu14I TTCGAA 1 cut(s) 398
BpuEI CTTGAG 5 cut(s) 83, 1557, 3167, 3673, 3734
BpuMI CCSGG 1 cut(s) 3593
BsaAI YACGTR 1 cut(s) 3176
BsaBI GATNNNNATC 2 cut(s) 3909, 4306
BsaHI GRCGYC 1 cut(s) 13
BsaI GGTCTC 2 cut(s) 225, 1156
BsaWI WCCGGW 2 cut(s) 373, 4288
BsaXI ACNNNNNCTCC 4 cut(s) 266, 296, 3328, 3358
Bsc4I CCNNNNNNNGG 8 cut(s) 22, 877, 1792, 1867, 2003, 2735, 3010, 3257
Bse118I RCCGGY 1 cut(s) 3075
Bse21I CCTNAGG 1 cut(s) 1653
Bse3DI GCAATG 2 cut(s) 3361, 3939
Bse8I GATNNNNATC 2 cut(s) 3909, 4306
BseAI TCCGGA 1 cut(s) 4288
BseBI CCWGG 8 cut(s) 492, 503, 1847, 1862, 2476, 3240, 3415, 3920
BseJI GATNNNNATC 2 cut(s) 3909, 4306
BseLI CCNNNNNNNGG 8 cut(s) 22, 877, 1792, 1867, 2003, 2735, 3010, 3257
BseMI GCAATG 2 cut(s) 3361, 3939
BseRI GAGGAG 9 cut(s) 25, 28, 254, 444, 1355, 1492, 1907, 3474, 4022
BseSI GKGCMC 2 cut(s) 2287, 2499
BseX3I CGGCCG 1 cut(s) 4355
BseYI CCCAGC 1 cut(s) 2072
BsgI GTGCAG 3 cut(s) 336, 549, 4067
Bsh1236I CGCG 3 cut(s) 189, 3445, 4354
Bsh1285I CGRYCG 2 cut(s) 187, 4358
BshNI GGYRCC 1 cut(s) 12
BsiEI CGRYCG 2 cut(s) 187, 4358
BsiHKAI GWGCWC 4 cut(s) 2397, 2499, 3346, 3946
BsiHKCI CYCGRG 3 cut(s) 2761, 4097, 4519
BsiSI CCGG 6 cut(s) 95, 374, 2131, 3076, 3593, 4289
BslFI GGGAC 6 cut(s) 274, 763, 2777, 2986, 3086, 4502
BslI CCNNNNNNNGG 8 cut(s) 22, 877, 1792, 1867, 2003, 2735, 3010, 3257
BsmFI GGGAC 6 cut(s) 274, 763, 2777, 2986, 3086, 4502
BsmI GAATGC 2 cut(s) 2413, 3140
Bso31I GGTCTC 2 cut(s) 225, 1156
BsoBI CYCGRG 3 cut(s) 2761, 4097, 4519
Bsp119I TTCGAA 1 cut(s) 398
Bsp120I GGGCCC 1 cut(s) 2283
Bsp1286I GDGCHC 6 cut(s) 762, 2287, 2397, 2499, 3346, 3946
Bsp13I TCCGGA 1 cut(s) 4288
Bsp1407I TGTACA 1 cut(s) 2752
Bsp1720I GCTNAGC 2 cut(s) 2213, 2562
Bsp19I CCATGG 2 cut(s) 1866, 3117
BspEI TCCGGA 1 cut(s) 4288
BspFNI CGCG 3 cut(s) 189, 3445, 4354
BspHI TCATGA 1 cut(s) 2323
BspMAI CTGCAG 3 cut(s) 2848, 3010, 3880
BspMI ACCTGC 1 cut(s) 4498
BspPI GGATC 8 cut(s) 687, 700, 1010, 3003, 3769, 3786, 3930, 4418
BspQI GCTCTTC 1 cut(s) 3543
BspT104I TTCGAA 1 cut(s) 398
BspT107I GGYRCC 1 cut(s) 12
BspTNI GGTCTC 2 cut(s) 225, 1156
BsrBI CCGCTC 1 cut(s) 3046
BsrDI GCAATG 2 cut(s) 3361, 3939
BsrFI RCCGGY 1 cut(s) 3075
BsrGI TGTACA 1 cut(s) 2752
BssAI RCCGGY 1 cut(s) 3075
BssNAI GTATAC 1 cut(s) 4205
BssNI GRCGYC 1 cut(s) 13
BssT1I CCWWGG 4 cut(s) 21, 151, 1866, 3117
Bst1107I GTATAC 1 cut(s) 4205
Bst2UI CCWGG 8 cut(s) 492, 503, 1847, 1862, 2476, 3240, 3415, 3920
Bst6I CTCTTC 9 cut(s) 59, 1051, 1075, 1259, 1998, 2724, 3416, 3543, 4460
BstACI GRCGYC 1 cut(s) 13
BstAPI GCANNNNNTGC 1 cut(s) 3014
BstAUI TGTACA 1 cut(s) 2752
BstBAI YACGTR 1 cut(s) 3176
BstBI TTCGAA 1 cut(s) 398
BstC8I GCNNGC 2 cut(s) 1127, 1331
BstDSI CCRYGG 3 cut(s) 1866, 3117, 4352
BstEII GGTNACC 1 cut(s) 4082
BstFNI CGCG 3 cut(s) 189, 3445, 4354
BstH2I RGCGCY 1 cut(s) 16
BstHHI GCGC 4 cut(s) 15, 520, 1329, 3445
BstMCI CGRYCG 2 cut(s) 187, 4358
BstNI CCWGG 8 cut(s) 492, 503, 1847, 1862, 2476, 3240, 3415, 3920
BstNSI RCATGY 2 cut(s) 1252, 4169
BstPAI GACNNNNGTC 1 cut(s) 1623
BstPI GGTNACC 1 cut(s) 4082
BstSCI CCNGG 9 cut(s) 490, 501, 1845, 1860, 2474, 3238, 3413, 3591, 3918
BstSFI CTRYAG 6 cut(s) 1458, 2844, 3006, 3730, 3876, 3926
BstSLI GKGCMC 2 cut(s) 2287, 2499
BstUI CGCG 3 cut(s) 189, 3445, 4354
BstV2I GAAGAC 2 cut(s) 1455, 2210
BstX2I RGATCY 6 cut(s) 692, 1015, 2995, 3197, 3761, 3922
BstXI CCANNNNNNTGG 1 cut(s) 3775
BstYI RGATCY 6 cut(s) 692, 1015, 2995, 3197, 3761, 3922
BstZ17I GTATAC 1 cut(s) 4205
BstZI CGGCCG 1 cut(s) 4355
Bsu36I CCTNAGG 1 cut(s) 1653
BtgI CCRYGG 3 cut(s) 1866, 3117, 4352
BtrI CACGTC 2 cut(s) 456, 2458
BtsI GCAGTG 3 cut(s) 2207, 2497, 4251
BtsIMutI CAGTG 6 cut(s) 235, 1793, 2207, 2497, 3606, 4251
BveI ACCTGC 1 cut(s) 4498
Cac8I GCNNGC 2 cut(s) 1127, 1331
CaiI CAGNNNCTG 3 cut(s) 1792, 3884, 4337
CciI TCATGA 1 cut(s) 2323
CfoI GCGC 4 cut(s) 15, 520, 1329, 3445
Cfr10I RCCGGY 1 cut(s) 3075
Cfr42I CCGCGG 1 cut(s) 4355
CseI GACGC 2 cut(s) 271, 1322
CsiI ACCWGGT 1 cut(s) 3238
CspCI CAANNNNNGTGG 2 cut(s) 2563, 2598
DinI GGCGCC 1 cut(s) 14
DraI TTTAAA 1 cut(s) 1818
DraIII CACNNNGTG 1 cut(s) 527
EaeI YGGCCR 1 cut(s) 4355
EagI CGGCCG 1 cut(s) 4355
Eam1104I CTCTTC 9 cut(s) 59, 1051, 1075, 1259, 1998, 2724, 3416, 3543, 4460
EarI CTCTTC 9 cut(s) 59, 1051, 1075, 1259, 1998, 2724, 3416, 3543, 4460
EciI GGCGGA 1 cut(s) 2718
Ecl136II GAGCTC 2 cut(s) 3344, 3944
EclXI CGGCCG 1 cut(s) 4355
Eco130I CCWWGG 4 cut(s) 21, 151, 1866, 3117
Eco147I AGGCCT 3 cut(s) 800, 1782, 4391
Eco24I GRGCYC 4 cut(s) 762, 2287, 3346, 3946
Eco31I GGTCTC 2 cut(s) 225, 1156
Eco32I GATATC 1 cut(s) 2553
Eco47I GGWCC 7 cut(s) 4, 731, 2791, 3073, 3236, 3464, 4516
Eco52I CGGCCG 1 cut(s) 4355
Eco53kI GAGCTC 2 cut(s) 3344, 3944
Eco57I CTGAAG 6 cut(s) 767, 1467, 1568, 2505, 3524, 3608
Eco81I CCTNAGG 1 cut(s) 1653
Eco88I CYCGRG 3 cut(s) 2761, 4097, 4519
Eco91I GGTNACC 1 cut(s) 4082
EcoICRI GAGCTC 2 cut(s) 3344, 3944
EcoO109I RGGNCCY 4 cut(s) 1657, 1910, 2284, 2791
EcoO65I GGTNACC 1 cut(s) 4082
EcoRI GAATTC 3 cut(s) 196, 859, 2824
EcoRII CCWGG 8 cut(s) 490, 501, 1845, 1860, 2474, 3238, 3413, 3918
EcoRV GATATC 1 cut(s) 2553
EcoT14I CCWWGG 4 cut(s) 21, 151, 1866, 3117
EcoT38I GRGCYC 4 cut(s) 762, 2287, 3346, 3946
EgeI GGCGCC 1 cut(s) 14
EheI GGCGCC 1 cut(s) 14
ErhI CCWWGG 4 cut(s) 21, 151, 1866, 3117
FalI AAGNNNNNCTT 2 cut(s) 1743, 1775
FaqI GGGAC 6 cut(s) 274, 763, 2777, 2986, 3086, 4502
FauNDI CATATG 2 cut(s) 967, 2785
FblI GTMKAC 4 cut(s) 933, 2862, 3978, 4204
FriOI GRGCYC 4 cut(s) 762, 2287, 3346, 3946
FspBI CTAG 7 cut(s) 1238, 1605, 2108, 3263, 3339, 3857, 4385
FspI TGCGCA 1 cut(s) 1328
GlaI GCGC 4 cut(s) 14, 519, 1328, 3444
GsaI CCCAGC 1 cut(s) 2076
GsuI CTGGAG 4 cut(s) 1358, 1695, 1769, 2804
HaeII RGCGCY 1 cut(s) 16
HapII CCGG 6 cut(s) 95, 374, 2131, 3076, 3593, 4289
HgaI GACGC 2 cut(s) 271, 1322
HhaI GCGC 4 cut(s) 15, 520, 1329, 3445
Hin1I GRCGYC 1 cut(s) 13
Hin6I GCGC 4 cut(s) 13, 518, 1327, 3443
HinP1I GCGC 4 cut(s) 13, 518, 1327, 3443
HincII GTYRAC 2 cut(s) 1602, 3546
HindII GTYRAC 2 cut(s) 1602, 3546
HindIII AAGCTT 3 cut(s) 637, 2523, 3583
HpaI GTTAAC 2 cut(s) 1602, 3546
HpaII CCGG 6 cut(s) 95, 374, 2131, 3076, 3593, 4289
HphI GGTGA 9 cut(s) 628, 1112, 1198, 2126, 2714, 2892, 3430, 4094, 4235
Hpy99I CGWCG 2 cut(s) 2445, 4127
HpyCH4IV ACGT 5 cut(s) 455, 2457, 2834, 2972, 3175
HpySE526I ACGT 5 cut(s) 455, 2457, 2834, 2972, 3175
Hsp92I GRCGYC 1 cut(s) 13
HspAI GCGC 4 cut(s) 13, 518, 1327, 3443
KasI GGCGCC 1 cut(s) 12
Kpn2I TCCGGA 1 cut(s) 4288
KspAI GTTAAC 2 cut(s) 1602, 3546
KspI CCGCGG 1 cut(s) 4355
LguI GCTCTTC 1 cut(s) 3543
MabI ACCWGGT 1 cut(s) 3238
MaeI CTAG 7 cut(s) 1238, 1605, 2108, 3263, 3339, 3857, 4385
MaeII ACGT 5 cut(s) 455, 2457, 2834, 2972, 3175
MaeIII GTNAC 7 cut(s) 616, 1409, 1717, 2488, 3169, 3970, 4082
MbiI CCGCTC 1 cut(s) 3046
MflI RGATCY 6 cut(s) 692, 1015, 2995, 3197, 3761, 3922
MhlI GDGCHC 6 cut(s) 762, 2287, 2397, 2499, 3346, 3946
Mly113I GGCGCC 1 cut(s) 13
MlyI GAGTC 4 cut(s) 785, 2936, 3475, 3747
MmeI TCCRAC 6 cut(s) 72, 207, 2724, 2941, 3938, 4116
MroI TCCGGA 1 cut(s) 4288
MroXI GAANNNNTTC 5 cut(s) 306, 2126, 2429, 2874, 3031
MslI CAYNNNNRTG 4 cut(s) 2592, 2700, 3107, 3773
MspA1I CMGCKG 5 cut(s) 533, 884, 3875, 4354, 4502
MspI CCGG 6 cut(s) 95, 374, 2131, 3076, 3593, 4289
MspR9I CCNGG 9 cut(s) 492, 503, 1847, 1862, 2476, 3240, 3415, 3593, 3920
Mva1269I GAATGC 2 cut(s) 2413, 3140
MvaI CCWGG 8 cut(s) 492, 503, 1847, 1862, 2476, 3240, 3415, 3920
MvnI CGCG 3 cut(s) 189, 3445, 4354
NarI GGCGCC 1 cut(s) 13
NciI CCSGG 1 cut(s) 3593
NcoI CCATGG 2 cut(s) 1866, 3117
NdeI CATATG 2 cut(s) 967, 2785
NmeAIII GCCGAG 1 cut(s) 2494
NmuCI GTSAC 3 cut(s) 616, 1409, 4082
NsbI TGCGCA 1 cut(s) 1328
NspI RCATGY 2 cut(s) 1252, 4169
NspV TTCGAA 1 cut(s) 398
OliI CACNNNNGTG 1 cut(s) 2700
PagI TCATGA 1 cut(s) 2323
PasI CCCWGGG 1 cut(s) 3414
PceI AGGCCT 3 cut(s) 800, 1782, 4391
PciI ACATGT 1 cut(s) 4165
PciSI GCTCTTC 1 cut(s) 3543
PctI GAATGC 2 cut(s) 2413, 3140
PdmI GAANNNNTTC 5 cut(s) 306, 2126, 2429, 2874, 3031
PflFI GACNNNGTC 1 cut(s) 3791
PflMI CCANNNNNTGG 2 cut(s) 1792, 1867
PleI GAGTC 4 cut(s) 784, 2936, 3475, 3747
PluTI GGCGCC 1 cut(s) 16
PpsI GAGTC 4 cut(s) 784, 2936, 3475, 3747
Ppu21I YACGTR 1 cut(s) 3176
PpuMI RGGWCCY 1 cut(s) 2791
PscI ACATGT 1 cut(s) 4165
PshAI GACNNNNGTC 1 cut(s) 1623
Psp124BI GAGCTC 2 cut(s) 3346, 3946
Psp1406I AACGTT 2 cut(s) 2834, 2972
Psp5II RGGWCCY 1 cut(s) 2791
Psp6I CCWGG 8 cut(s) 490, 501, 1845, 1860, 2474, 3238, 3413, 3918
PspEI GGTNACC 1 cut(s) 4082
PspFI CCCAGC 1 cut(s) 2072
PspGI CCWGG 8 cut(s) 490, 501, 1845, 1860, 2474, 3238, 3413, 3918
PspOMI GGGCCC 1 cut(s) 2283
PspPPI RGGWCCY 1 cut(s) 2791
PsrI GAACNNNNNNTAC 4 cut(s) 379, 411, 972, 1004
PstI CTGCAG 3 cut(s) 2848, 3010, 3880
PstNI CAGNNNCTG 3 cut(s) 1792, 3884, 4337
PsuI RGATCY 6 cut(s) 692, 1015, 2995, 3197, 3761, 3922
PsyI GACNNNGTC 1 cut(s) 3791
PvuII CAGCTG 3 cut(s) 533, 884, 3875
RseI CAYNNNNRTG 4 cut(s) 2592, 2700, 3107, 3773
SacI GAGCTC 2 cut(s) 3346, 3946
SacII CCGCGG 1 cut(s) 4355
SapI GCTCTTC 1 cut(s) 3543
SchI GAGTC 4 cut(s) 785, 2936, 3475, 3747
ScrFI CCNGG 9 cut(s) 492, 503, 1847, 1862, 2476, 3240, 3415, 3593, 3920
SduI GDGCHC 6 cut(s) 762, 2287, 2397, 2499, 3346, 3946
SexAI ACCWGGT 1 cut(s) 3238
SfcI CTRYAG 6 cut(s) 1458, 2844, 3006, 3730, 3876, 3926
SfoI GGCGCC 1 cut(s) 14
Sfr303I CCGCGG 1 cut(s) 4355
SfuI TTCGAA 1 cut(s) 398
SgrBI CCGCGG 1 cut(s) 4355
SinI GGWCC 7 cut(s) 4, 731, 2791, 3073, 3236, 3464, 4516
SmiMI CAYNNNNRTG 4 cut(s) 2592, 2700, 3107, 3773
SmlI CTYRAG 5 cut(s) 62, 1572, 3146, 3652, 3713
SmoI CTYRAG 5 cut(s) 62, 1572, 3146, 3652, 3713
SpeI ACTAGT 1 cut(s) 1604
SseBI AGGCCT 3 cut(s) 800, 1782, 4391
SspDI GGCGCC 1 cut(s) 12
SspI AATATT 5 cut(s) 1519, 2623, 2925, 3298, 3709
SspMI CTAG 7 cut(s) 1238, 1605, 2108, 3263, 3339, 3857, 4385
SstI GAGCTC 2 cut(s) 3346, 3946
StuI AGGCCT 3 cut(s) 800, 1782, 4391
StyD4I CCNGG 9 cut(s) 490, 501, 1845, 1860, 2474, 3238, 3413, 3591, 3918
StyI CCWWGG 4 cut(s) 21, 151, 1866, 3117
TaiI ACGT 5 cut(s) 458, 2460, 2837, 2975, 3178
TatI WGTACW 3 cut(s) 315, 2752, 3839
TauI GCSGC 3 cut(s) 3049, 4357, 4505
TscAI CASTG 6 cut(s) 235, 1793, 2214, 2504, 3613, 4258
TseFI GTSAC 3 cut(s) 616, 1409, 4082
Tsp45I GTSAC 3 cut(s) 616, 1409, 4082
TspGWI ACGGA 3 cut(s) 287, 2429, 3299
TspRI CASTG 6 cut(s) 235, 1793, 2214, 2504, 3613, 4258
Tth111I GACNNNGTC 1 cut(s) 3791
Van91I CCANNNNNTGG 2 cut(s) 1792, 1867
VneI GTGCAC 1 cut(s) 2495
VpaK11BI GGWCC 7 cut(s) 4, 731, 2791, 3073, 3236, 3464, 4516
XapI RAATTY 6 cut(s) 196, 859, 2604, 2713, 2824, 3555
XceI RCATGY 2 cut(s) 1252, 4169
XcmI CCANNNNNNNNNTGG 2 cut(s) 1864, 1873
XmiI GTMKAC 4 cut(s) 933, 2862, 3978, 4204
XmnI GAANNNNTTC 5 cut(s) 306, 2126, 2429, 2874, 3031
XspI CTAG 7 cut(s) 1238, 1605, 2108, 3263, 3339, 3857, 4385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.