Rh3CG291800

DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0005736
Physical Location & Seq
Forward (+)
29699686 .. 29711336
11651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG291800.1

Sequence Viewer

Length: 4017 bp
ATGGACCACAGGCGCCAATCCAAGGATTCTCTCTCCTCCTCCTACTCCAACCTCTTCAATCTTGAGCCTTTGATGAACTTTCAGCTTCCACAGCCAGATGATGATTTTGATTATTACGGCAATAGCAGTCAGGATGAGAGCAGAGGTAGCCAAGGTGGAGCTGCTGGTAATGGGATTATGTCTGACCGCGAACTGAATTCAGTAAAGAAAAGGAGGCGGTCTCAAAACAGTGATTACGAGGAGGATGACAGTTATTACGGGACGCACATTACGGAGGACAAGTATAGATCAATGCTTGGAGAACACATTCAGAAGTACAAAAGGAGGTTCAAGGATTCCCCGTCAAGTCCTGCACCAATCCAGAGGGGGATTCCGGTTTCAAAGGGTAATAAGGGTTCGAAATCGAGGAAGCTGGCGAATGAGAACCGAGGAGGGTTTTATGAAATGGAAACCACGTCTGAGTGGCTCAATGATACTATTCCTCAGAAACCAGGGAACTACCATGATGCTGATTTTGCGCCACAAAGTGCCGCTGATAGAGTAATATATGAACCTCCTTATCTGGATATTGGTGATGGTTTCACATACAAGATTCCACCAATTTATGATAAACTGGTGACATCTTTACACTTGCCGAGCTTTTCTGATTTTCGGGTGGAGGAAGTTTACTTAAAAGGTACATTGGATTTGGGATCCTTAGCAGAAATGATGGGTAGCGATAAAAGGTTTGGTCCAAAAAATCGGGCAGGAATGGGGGAGCCCTATCCCCAGTATGAGTCCCTTCAGGCAAGATTAAAGGCCTTGTCCACTTCTACCTCTGCTCAAAATTTCAGCCTCAAAGTTTCTGATATTGGGTTGAATTCCTCCATCCCAGAGGGGGCAGCTGGAACTATAAAGAGGTCTATTTTGTCCGAGGGTGGTGTCTTGCAAGTCTACTATGTGAAGGTTTTGGAGAAGGGAGACACATATGAGATAATTGAACGAAGCCTACCCAAGAAGCAAAAGGTACAGAAAGATCCTTCTCAGATTGAGAAGGAGGAAATGGAAAGGATGGGAAGAGTTTGGGTAAACATTGTAAGAAGAGACATACCAAAGCATCATAGAATGTTTACTACTTTTCACCGCAAGCAACTAATTGATGCCAAGAGGGTCTCAGAGAACTGTCAAAGAGAGGTAAAAATGAAGGTGAGTAGATCACTTAAAGTGATGAGGGGTGCTGCAATTCGCACAAGGAAACTAGCCAGAGACATGCTACTGCTTTGGAAGAGAATAGATAAAGAGATGGCAGAAGTGAGGAAGAAAGAGGAAAAAGAAGCTATTGAAATGCGCAAACGTCTGGAGGAGGAAAGAGAAGCAAAGAGACAGGAGCAAAGACTCAATTTTCTCATAAAACAAACCGAACTTTACAGTCACTTCATGCAGAACAAGTTGAGTTCACAGCCATCTGAAGACTTGCCTGTAGGAGATGAAAACCAAGATGTATCTCCAAGCTCCTCAGATATTGAGAATGTTGAAGAAGATTCTGAGGAAGCTGAACTGAAGAAGGAGGCATTAAAAGCTGCTCAAGATGCAGTTTCAAAGCAGAAAAAGTTAACTAGTGCATTTGATGACGAGTGTCTGAGGCTGCGTGAAGCCTCTGAACCTGAGGCACCACAGGATGTTGCTGGAGCTAATAACATTGATCTGCATAACCCTTCCACTATGCCTGTTACATCAACCGTTCAGACACCAGAGTTGTTCAAAGGCTCCCTTAAAGAATACCAGCTAAAAGGCCTCCAGTGGCTGGTTAATTGTTATGAGCAGGGTTTAAATGGCATCCTTGCTGATGAGATGGGCCTGGGTAAGACCATCCAGGCCATGGCATTTTTGGCTCATTTGGCTGAGGAGAAAAATATATGGGGGCCTTTTCTGGTTGTTGCTCCTGCTTCCGTCTTGAACAACTGGGCTGATGAAATTAGTCGTTTCTGCCCCGACTTAAAAACTCTTCCGTATTGGGGTGGCCTTCAAGAGCGTATGGTACTTAGGAAGAAGATCAACGCTAAGAAACTTTACCGTAGGGATGCTGGGTTTCACATTCTCATTACCAGCTACCAGTTGCTAGTGGGTGATGAGAAGTGTTTCCGGCGTGTGAAATGGCAATATATGGTATTGGATGAAGCACAAGCTATCAAAAGTTCTAACAGTATAAGATGGAAGACACTGCTCAGCTTTAATTGTCGAAATCGTTTGCTGCTTACTGGTACACCTATCCAGAATAATATGGCAGAGTTGTGGGCCCTACTACATTTCATTATGCCAACCTTATTTGACAGTCATGAACAATTCAATGAGTGGTTCTCTAAAGGAATTGAAAATCATGCGGAACATGGGGGTACTTTGAACGAGCACCAGCTTAACAGATTGCATTCGATATTGAAGCCATTCATGCTCCGTCGAGTTAAGACAGATGTGATTTCTGAGCTGACCAGGAAAACTGAAGTTACTGTGCACTGCAAGTTGAGTTCTCGGCAACAAGCTTTTTATCAAGCTATCAAAAACAAGATATCTCTTGCTGAGCTGTTTGACAACAATCGTGGGCATCTTAATGAGAAGAAAATTTTGAACTTAATGAATATTGTCATTCAGCTAAGAAAGGTCTGCAACCATCCTGAGTTATTTGAGAGGAATGAAGGAAGCACATATCTTCACTTTGGTGAGATTTCAAATTCTCTTCTTCCTCCACCATTTGGGGAGTTGGAGGACGTACACTACTCGGGAGGTCAAAATCCTATAACATACGAGGTCCCCAAACTTTTATATCAAGAAATACTCCAGAATTCTGAAACGTTTTGCTCTGCAGTTCGGCATGGTGTCTACAGTGAATCTTTTCAGAAACATTTTAACATATTTTCACCGCAAAATGTGTATCGATCAATATTCTTGCAAGAGAATCACTCAGATGAGTTGTCGGTTGGAAGTGGAACGTTTGGTTTTACTCATCTGATGGATCTGTCCCCTGCAGAGGTTGCATTTTTAGGAACTGGTTCTTTTATGGAGCAGCTAATGTTTTCCATTATGAGATGGGACCGGCAGTTTTTGGATGGATTGATAGACACACTAATGGAAACCGAGGATGATGATGATTCTGAATGCAGCTTCCTTGAGAGTGGAAAAGTGAGAGCTGTTACACGTATGTTGCTGATGCCTTCAAGATCTATAACTACCATCTTTCAGAAGAAATTGGCCACTGGACCTGGTGGTACTCCATTTGAGGGTCTAGTTGTCTCTCATCGGGACAGGCTTCTCTCTAATATTAGGCTTCTCCGTTCGACATACACATTCATCCCCCGAACTAGAGCTCCCCCAGTTAATGTCCATTGCTCAGATCGAAACTTCACTTACAAAATGAGTGAAGAACAACAGTACCCCTGGGTGAAGAGGTTGTTTTCTGGTTTTGCGCGAACATCTGACTACAATGGACCCAGAAAACCTGAGACTCCTCATCATCTAATACAAGAGATTGATTCTGAACTGCCTGTTTCACATTCAGCTCTTCAGTTGACATACAGAATTTTTGGGTCTTGTCCTCCAATGCAAAGCTTCGACCCGTCAAAAATGCTCACTGATTCGGGGAAACTTCAGACACTTGATATATTGTTGAAACGCTTGAGGGCAGAAAATCATCGTGTTCTCTTGTTTGCACAGATGACAAAGATGCTGAATATTCTTGAGGACTACATGAACTACAGGAAATATAGATACCTCAGACTGGATGGATCTTCCACCATTATGGATCGTAGAGACATGGTCAGAGACTTCCAGCAACGAAGTGATATTTTTGTTTTCTTGCTGAGTACAAGAGCTGGTGGACTAGGCATTAACTTGACAGCTGCAGATACTGTCATATTTTATGAAAGTGATTGGAATCCGACCCTGGATCTACAGGCAATGGATAGAGCTCATCGGTTGGGCCAGACTAAAGATGTGAGTTTCTTTTTGCTAATCCAGCGTACACCAATCCGCTCCCAAATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBINO Helicase_C SNF2_N

Protein Analysis

1338

Amino Acids

153.74

Weight (kDa)

8.01

Isoelectric Point (pI)

52.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBINO PF13892 349 - 479 1.1e-44 DNA-binding domain
ResIII PF04851 583 - 750 9.3e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 587 - 885 3.3e-85 SNF2-related domain
Helicase_C PF00271 1207 - 1317 2.3e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1328
AccB1I GGYRCC 2 cut(s) 12, 1648
AccB7I CCANNNNNTGG 3 cut(s) 1783, 1858, 2726
AccBSI CCGCTC 1 cut(s) 4002
AccI GTMKAC 2 cut(s) 933, 2853
AccII CGCG 2 cut(s) 189, 3439
AciI CCGC 7 cut(s) 187, 217, 531, 1123, 2360, 2894, 4000
AclI AACGTT 2 cut(s) 2825, 2963
AclWI GGATC 7 cut(s) 687, 700, 1010, 2994, 3763, 3780, 3924
AcoI YGGCCR 1 cut(s) 3222
AcsI RAATTY 7 cut(s) 196, 826, 859, 2595, 2704, 2815, 3549
AcuI CTGAAG 6 cut(s) 767, 1467, 1559, 2496, 3518, 3602
AcyI GRCGYC 1 cut(s) 13
AdeI CACNNNGTG 1 cut(s) 527
AfiI CCNNNNNNNGG 9 cut(s) 22, 877, 1783, 1858, 1994, 2726, 3001, 3251, 3748
AflIII ACRYGT 1 cut(s) 3167
AhlI ACTAGT 1 cut(s) 1595
AjiI CACGTC 1 cut(s) 456
AjnI CCWGG 7 cut(s) 490, 1836, 1851, 2465, 3232, 3407, 3912
AleI CACNNNNGTG 1 cut(s) 2691
AloI GAACNNNNNNTCC 2 cut(s) 3322, 3354
Alw21I GWGCWC 4 cut(s) 2388, 2490, 3340, 3940
Alw44I GTGCAC 1 cut(s) 2486
AlwI GGATC 7 cut(s) 687, 700, 1010, 2994, 3763, 3780, 3924
AlwNI CAGNNNCTG 2 cut(s) 1783, 3878
Ama87I CYCGRG 1 cut(s) 2752
AoxI GGCC 9 cut(s) 798, 1771, 1834, 1854, 1901, 1999, 2274, 3222, 3949
ApaI GGGCCC 1 cut(s) 2278
ApaLI GTGCAC 1 cut(s) 2486
ApeKI GCWGC 9 cut(s) 161, 881, 1217, 1559, 1624, 2230, 3037, 3132, 3869
ApoI RAATTY 7 cut(s) 196, 826, 859, 2595, 2704, 2815, 3549
Asp700I GAANNNNTTC 5 cut(s) 306, 2117, 2420, 2865, 3022
AspLEI GCGC 4 cut(s) 15, 520, 1329, 3439
AsuHPI GGTGA 8 cut(s) 584, 628, 1112, 1198, 2117, 2705, 2883, 3424
AsuII TTCGAA 1 cut(s) 398
AvaI CYCGRG 1 cut(s) 2752
AvaII GGWCC 6 cut(s) 4, 731, 2782, 3064, 3230, 3458
AxyI CCTNAGG 1 cut(s) 1644
BaeGI GKGCMC 2 cut(s) 2278, 2490
BaeI ACNNNNGTAYC 2 cut(s) 3386, 3419
BalI TGGCCA 1 cut(s) 3224
BamHI GGATCC 1 cut(s) 692
BanI GGYRCC 2 cut(s) 12, 1648
BanII GRGCYC 4 cut(s) 762, 2278, 3340, 3940
BbsI GAAGAC 2 cut(s) 1455, 2201
Bbv12I GWGCWC 4 cut(s) 2388, 2490, 3340, 3940
BbvCI CCTCAGC 1 cut(s) 1881
BbvI GCAGC 9 cut(s) 148, 893, 1204, 1546, 1611, 2217, 3049, 3144, 3856
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 7 cut(s) 492, 1838, 1853, 2467, 3234, 3409, 3914
BcuI ACTAGT 1 cut(s) 1595
BfaI CTAG 6 cut(s) 1238, 1596, 2099, 3257, 3333, 3851
BfmI CTRYAG 7 cut(s) 1458, 2835, 2854, 2997, 3724, 3870, 3920
BfoI RGCGCY 1 cut(s) 16
BglII AGATCT 1 cut(s) 3191
BlpI GCTNAGC 2 cut(s) 2204, 2553
Bme1390I CCNGG 7 cut(s) 492, 1838, 1853, 2467, 3234, 3409, 3914
Bme18I GGWCC 6 cut(s) 4, 731, 2782, 3064, 3230, 3458
BmeT110I CYCGRG 1 cut(s) 2752
BmgBI CACGTC 1 cut(s) 456
BmrFI CCNGG 7 cut(s) 492, 1838, 1853, 2467, 3234, 3409, 3914
BmrI ACTGGG 3 cut(s) 763, 1951, 3338
BmsI GCATC 9 cut(s) 496, 1105, 1129, 1558, 1824, 2050, 2587, 3171, 3684
BmuI ACTGGG 3 cut(s) 763, 1951, 3338
BoxI GACNNNNGTC 1 cut(s) 1614
BpiI GAAGAC 2 cut(s) 1455, 2201
BplI GAGNNNNNCTC 6 cut(s) 205, 237, 2321, 2353, 2918, 2950
BpmI CTGGAG 4 cut(s) 1358, 1686, 1760, 2795
Bpu10I CCTNAGC 2 cut(s) 697, 1881
Bpu1102I GCTNAGC 2 cut(s) 2204, 2553
Bpu14I TTCGAA 1 cut(s) 398
BpuEI CTTGAG 5 cut(s) 83, 1548, 3161, 3667, 3728
Bsa29I ATCGAT 1 cut(s) 2908
BsaAI YACGTR 1 cut(s) 3170
BsaBI GATNNNNATC 1 cut(s) 3903
BsaHI GRCGYC 1 cut(s) 13
BsaI GGTCTC 2 cut(s) 225, 1156
BsaWI WCCGGW 1 cut(s) 373
BsaXI ACNNNNNCTCC 4 cut(s) 266, 296, 3322, 3352
Bsc4I CCNNNNNNNGG 9 cut(s) 22, 877, 1783, 1858, 1994, 2726, 3001, 3251, 3748
Bse118I RCCGGY 1 cut(s) 3066
Bse21I CCTNAGG 1 cut(s) 1644
Bse3DI GCAATG 2 cut(s) 3355, 3933
Bse8I GATNNNNATC 1 cut(s) 3903
BseBI CCWGG 7 cut(s) 492, 1838, 1853, 2467, 3234, 3409, 3914
BseCI ATCGAT 1 cut(s) 2908
BseJI GATNNNNATC 1 cut(s) 3903
BseLI CCNNNNNNNGG 9 cut(s) 22, 877, 1783, 1858, 1994, 2726, 3001, 3251, 3748
BseMI GCAATG 2 cut(s) 3355, 3933
BseRI GAGGAG 8 cut(s) 25, 28, 254, 444, 1355, 1483, 1898, 3468
BseSI GKGCMC 2 cut(s) 2278, 2490
BseXI GCAGC 9 cut(s) 148, 893, 1204, 1546, 1611, 2217, 3049, 3144, 3856
BseYI CCCAGC 1 cut(s) 2063
BsgI GTGCAG 1 cut(s) 336
Bsh1236I CGCG 2 cut(s) 189, 3439
BshFI GGCC 9 cut(s) 800, 1773, 1836, 1856, 1903, 2001, 2276, 3224, 3951
BshNI GGYRCC 2 cut(s) 12, 1648
BshVI ATCGAT 1 cut(s) 2908
BsiHKAI GWGCWC 4 cut(s) 2388, 2490, 3340, 3940
BsiHKCI CYCGRG 1 cut(s) 2752
BsiSI CCGG 3 cut(s) 374, 2122, 3067
BslFI GGGAC 6 cut(s) 274, 763, 2768, 2977, 3077, 3287
BslI CCNNNNNNNGG 9 cut(s) 22, 877, 1783, 1858, 1994, 2726, 3001, 3251, 3748
BsmFI GGGAC 6 cut(s) 274, 763, 2768, 2977, 3077, 3287
BsmI GAATGC 2 cut(s) 2404, 3134
BsnI GGCC 9 cut(s) 800, 1773, 1836, 1856, 1903, 2001, 2276, 3224, 3951
Bso31I GGTCTC 2 cut(s) 225, 1156
BsoBI CYCGRG 1 cut(s) 2752
Bsp119I TTCGAA 1 cut(s) 398
Bsp120I GGGCCC 1 cut(s) 2274
Bsp1286I GDGCHC 6 cut(s) 762, 2278, 2388, 2490, 3340, 3940
Bsp1720I GCTNAGC 2 cut(s) 2204, 2553
Bsp19I CCATGG 1 cut(s) 1857
BspACI CCGC 7 cut(s) 187, 217, 531, 1123, 2360, 2894, 4000
BspANI GGCC 9 cut(s) 800, 1773, 1836, 1856, 1903, 2001, 2276, 3224, 3951
BspDI ATCGAT 1 cut(s) 2908
BspFNI CGCG 2 cut(s) 189, 3439
BspHI TCATGA 1 cut(s) 2314
BspMAI CTGCAG 3 cut(s) 2839, 3001, 3874
BspPI GGATC 7 cut(s) 687, 700, 1010, 2994, 3763, 3780, 3924
BspQI GCTCTTC 1 cut(s) 3537
BspT104I TTCGAA 1 cut(s) 398
BspT107I GGYRCC 2 cut(s) 12, 1648
BspTNI GGTCTC 2 cut(s) 225, 1156
BsrBI CCGCTC 1 cut(s) 4002
BsrDI GCAATG 2 cut(s) 3355, 3933
BsrFI RCCGGY 1 cut(s) 3066
BssAI RCCGGY 1 cut(s) 3066
BssNI GRCGYC 1 cut(s) 13
BssT1I CCWWGG 3 cut(s) 21, 151, 1857
Bst2UI CCWGG 7 cut(s) 492, 1838, 1853, 2467, 3234, 3409, 3914
Bst6I CTCTTC 8 cut(s) 59, 1051, 1075, 1259, 1989, 2715, 3410, 3537
BstACI GRCGYC 1 cut(s) 13
BstAPI GCANNNNNTGC 1 cut(s) 3005
BstBAI YACGTR 1 cut(s) 3170
BstBI TTCGAA 1 cut(s) 398
BstC8I GCNNGC 2 cut(s) 414, 1127
BstDSI CCRYGG 1 cut(s) 1857
BstFNI CGCG 2 cut(s) 189, 3439
BstH2I RGCGCY 1 cut(s) 16
BstHHI GCGC 4 cut(s) 15, 520, 1329, 3439
BstNI CCWGG 7 cut(s) 492, 1838, 1853, 2467, 3234, 3409, 3914
BstNSI RCATGY 1 cut(s) 1252
BstPAI GACNNNNGTC 1 cut(s) 1614
BstSCI CCNGG 7 cut(s) 490, 1836, 1851, 2465, 3232, 3407, 3912
BstSFI CTRYAG 7 cut(s) 1458, 2835, 2854, 2997, 3724, 3870, 3920
BstSLI GKGCMC 2 cut(s) 2278, 2490
BstUI CGCG 2 cut(s) 189, 3439
BstV1I GCAGC 9 cut(s) 148, 893, 1204, 1546, 1611, 2217, 3049, 3144, 3856
BstV2I GAAGAC 2 cut(s) 1455, 2201
BstX2I RGATCY 6 cut(s) 692, 1015, 2986, 3191, 3755, 3916
BstXI CCANNNNNNTGG 1 cut(s) 3769
BstYI RGATCY 6 cut(s) 692, 1015, 2986, 3191, 3755, 3916
Bsu15I ATCGAT 1 cut(s) 2908
Bsu36I CCTNAGG 1 cut(s) 1644
BsuRI GGCC 9 cut(s) 800, 1773, 1836, 1856, 1903, 2001, 2276, 3224, 3951
BsuTUI ATCGAT 1 cut(s) 2908
BtgI CCRYGG 1 cut(s) 1857
BtrI CACGTC 1 cut(s) 456
BtsI GCAGTG 2 cut(s) 2198, 2488
BtsIMutI CAGTG 7 cut(s) 235, 1784, 2198, 2488, 2863, 3225, 3600
Cac8I GCNNGC 2 cut(s) 414, 1127
CaiI CAGNNNCTG 2 cut(s) 1783, 3878
CciI TCATGA 1 cut(s) 2314
CfoI GCGC 4 cut(s) 15, 520, 1329, 3439
Cfr10I RCCGGY 1 cut(s) 3066
ClaI ATCGAT 1 cut(s) 2908
CseI GACGC 1 cut(s) 271
CsiI ACCWGGT 1 cut(s) 3232
CspCI CAANNNNNGTGG 2 cut(s) 2554, 2589
DinI GGCGCC 1 cut(s) 14
DraI TTTAAA 1 cut(s) 1809
DraIII CACNNNGTG 1 cut(s) 527
EaeI YGGCCR 1 cut(s) 3222
Eam1104I CTCTTC 8 cut(s) 59, 1051, 1075, 1259, 1989, 2715, 3410, 3537
EarI CTCTTC 8 cut(s) 59, 1051, 1075, 1259, 1989, 2715, 3410, 3537
Ecl136II GAGCTC 2 cut(s) 3338, 3938
Eco130I CCWWGG 3 cut(s) 21, 151, 1857
Eco147I AGGCCT 2 cut(s) 800, 1773
Eco24I GRGCYC 4 cut(s) 762, 2278, 3340, 3940
Eco31I GGTCTC 2 cut(s) 225, 1156
Eco32I GATATC 1 cut(s) 2544
Eco47I GGWCC 6 cut(s) 4, 731, 2782, 3064, 3230, 3458
Eco53kI GAGCTC 2 cut(s) 3338, 3938
Eco57I CTGAAG 6 cut(s) 767, 1467, 1559, 2496, 3518, 3602
Eco81I CCTNAGG 1 cut(s) 1644
Eco88I CYCGRG 1 cut(s) 2752
EcoICRI GAGCTC 2 cut(s) 3338, 3938
EcoO109I RGGNCCY 3 cut(s) 1901, 2275, 2782
EcoRI GAATTC 3 cut(s) 196, 859, 2815
EcoRII CCWGG 7 cut(s) 490, 1836, 1851, 2465, 3232, 3407, 3912
EcoRV GATATC 1 cut(s) 2544
EcoT14I CCWWGG 3 cut(s) 21, 151, 1857
EcoT38I GRGCYC 4 cut(s) 762, 2278, 3340, 3940
EgeI GGCGCC 1 cut(s) 14
EheI GGCGCC 1 cut(s) 14
ErhI CCWWGG 3 cut(s) 21, 151, 1857
FalI AAGNNNNNCTT 2 cut(s) 1734, 1766
FaqI GGGAC 6 cut(s) 274, 763, 2768, 2977, 3077, 3287
FauNDI CATATG 1 cut(s) 967
FblI GTMKAC 2 cut(s) 933, 2853
FriOI GRGCYC 4 cut(s) 762, 2278, 3340, 3940
FspBI CTAG 6 cut(s) 1238, 1596, 2099, 3257, 3333, 3851
FspI TGCGCA 1 cut(s) 1328
GlaI GCGC 4 cut(s) 14, 519, 1328, 3438
GsaI CCCAGC 1 cut(s) 2067
GsuI CTGGAG 4 cut(s) 1358, 1686, 1760, 2795
HaeII RGCGCY 1 cut(s) 16
HaeIII GGCC 9 cut(s) 800, 1773, 1836, 1856, 1903, 2001, 2276, 3224, 3951
HapII CCGG 3 cut(s) 374, 2122, 3067
HgaI GACGC 1 cut(s) 271
HhaI GCGC 4 cut(s) 15, 520, 1329, 3439
Hin1I GRCGYC 1 cut(s) 13
Hin6I GCGC 4 cut(s) 13, 518, 1327, 3437
HinP1I GCGC 4 cut(s) 13, 518, 1327, 3437
HincII GTYRAC 2 cut(s) 1593, 3540
HindII GTYRAC 2 cut(s) 1593, 3540
HindIII AAGCTT 2 cut(s) 2514, 3577
HpaI GTTAAC 1 cut(s) 1593
HpaII CCGG 3 cut(s) 374, 2122, 3067
HphI GGTGA 8 cut(s) 584, 628, 1112, 1198, 2117, 2705, 2883, 3424
Hpy99I CGWCG 1 cut(s) 2436
HpyCH4IV ACGT 6 cut(s) 455, 1333, 2742, 2825, 2963, 3169
HpySE526I ACGT 6 cut(s) 455, 1333, 2742, 2825, 2963, 3169
Hsp92I GRCGYC 1 cut(s) 13
HspAI GCGC 4 cut(s) 13, 518, 1327, 3437
KasI GGCGCC 1 cut(s) 12
KspAI GTTAAC 1 cut(s) 1593
LguI GCTCTTC 1 cut(s) 3537
Lsp1109I GCAGC 9 cut(s) 148, 893, 1204, 1546, 1611, 2217, 3049, 3144, 3856
LweI GCATC 9 cut(s) 496, 1105, 1129, 1558, 1824, 2050, 2587, 3171, 3684
MabI ACCWGGT 1 cut(s) 3232
MaeI CTAG 6 cut(s) 1238, 1596, 2099, 3257, 3333, 3851
MaeII ACGT 6 cut(s) 455, 1333, 2742, 2825, 2963, 3169
MaeIII GTNAC 5 cut(s) 616, 1409, 1708, 2479, 3163
MbiI CCGCTC 1 cut(s) 4002
MflI RGATCY 6 cut(s) 692, 1015, 2986, 3191, 3755, 3916
MhlI GDGCHC 6 cut(s) 762, 2278, 2388, 2490, 3340, 3940
MlsI TGGCCA 1 cut(s) 3224
MluNI TGGCCA 1 cut(s) 3224
Mly113I GGCGCC 1 cut(s) 13
MlyI GAGTC 3 cut(s) 785, 1368, 3469
MmeI TCCRAC 4 cut(s) 72, 2715, 2932, 3932
Mox20I TGGCCA 1 cut(s) 3224
MroXI GAANNNNTTC 5 cut(s) 306, 2117, 2420, 2865, 3022
MscI TGGCCA 1 cut(s) 3224
MslI CAYNNNNRTG 5 cut(s) 2583, 2691, 2937, 3098, 3767
Msp20I TGGCCA 1 cut(s) 3224
MspA1I CMGCKG 3 cut(s) 533, 884, 3869
MspI CCGG 3 cut(s) 374, 2122, 3067
MspR9I CCNGG 7 cut(s) 492, 1838, 1853, 2467, 3234, 3409, 3914
Mva1269I GAATGC 2 cut(s) 2404, 3134
MvaI CCWGG 7 cut(s) 492, 1838, 1853, 2467, 3234, 3409, 3914
MvnI CGCG 2 cut(s) 189, 3439
NarI GGCGCC 1 cut(s) 13
NcoI CCATGG 1 cut(s) 1857
NdeI CATATG 1 cut(s) 967
NmeAIII GCCGAG 2 cut(s) 660, 2485
NmuCI GTSAC 2 cut(s) 616, 1409
NsbI TGCGCA 1 cut(s) 1328
NspI RCATGY 1 cut(s) 1252
NspV TTCGAA 1 cut(s) 398
OliI CACNNNNGTG 1 cut(s) 2691
PagI TCATGA 1 cut(s) 2314
PasI CCCWGGG 1 cut(s) 3408
PceI AGGCCT 2 cut(s) 800, 1773
PciSI GCTCTTC 1 cut(s) 3537
PctI GAATGC 2 cut(s) 2404, 3134
PdmI GAANNNNTTC 5 cut(s) 306, 2117, 2420, 2865, 3022
PflFI GACNNNGTC 1 cut(s) 3785
PflMI CCANNNNNTGG 3 cut(s) 1783, 1858, 2726
PleI GAGTC 3 cut(s) 784, 1368, 3469
PluTI GGCGCC 1 cut(s) 16
PpsI GAGTC 3 cut(s) 784, 1368, 3469
Ppu21I YACGTR 1 cut(s) 3170
PpuMI RGGWCCY 1 cut(s) 2782
PshAI GACNNNNGTC 1 cut(s) 1614
Psp124BI GAGCTC 2 cut(s) 3340, 3940
Psp1406I AACGTT 2 cut(s) 2825, 2963
Psp5II RGGWCCY 1 cut(s) 2782
Psp6I CCWGG 7 cut(s) 490, 1836, 1851, 2465, 3232, 3407, 3912
PspFI CCCAGC 1 cut(s) 2063
PspGI CCWGG 7 cut(s) 490, 1836, 1851, 2465, 3232, 3407, 3912
PspOMI GGGCCC 1 cut(s) 2274
PspPPI RGGWCCY 1 cut(s) 2782
PsrI GAACNNNNNNTAC 4 cut(s) 379, 411, 972, 1004
PstI CTGCAG 3 cut(s) 2839, 3001, 3874
PstNI CAGNNNCTG 2 cut(s) 1783, 3878
PsuI RGATCY 6 cut(s) 692, 1015, 2986, 3191, 3755, 3916
PsyI GACNNNGTC 1 cut(s) 3785
PvuII CAGCTG 2 cut(s) 884, 3869
RseI CAYNNNNRTG 5 cut(s) 2583, 2691, 2937, 3098, 3767
SacI GAGCTC 2 cut(s) 3340, 3940
SapI GCTCTTC 1 cut(s) 3537
SchI GAGTC 3 cut(s) 785, 1368, 3469
ScrFI CCNGG 7 cut(s) 492, 1838, 1853, 2467, 3234, 3409, 3914
SduI GDGCHC 6 cut(s) 762, 2278, 2388, 2490, 3340, 3940
SexAI ACCWGGT 1 cut(s) 3232
SfaNI GCATC 9 cut(s) 496, 1105, 1129, 1558, 1824, 2050, 2587, 3171, 3684
SfcI CTRYAG 7 cut(s) 1458, 2835, 2854, 2997, 3724, 3870, 3920
SfoI GGCGCC 1 cut(s) 14
SfuI TTCGAA 1 cut(s) 398
SinI GGWCC 6 cut(s) 4, 731, 2782, 3064, 3230, 3458
SmiMI CAYNNNNRTG 5 cut(s) 2583, 2691, 2937, 3098, 3767
SmlI CTYRAG 5 cut(s) 62, 1563, 3140, 3646, 3707
SmoI CTYRAG 5 cut(s) 62, 1563, 3140, 3646, 3707
SpeI ACTAGT 1 cut(s) 1595
SseBI AGGCCT 2 cut(s) 800, 1773
SsiI CCGC 7 cut(s) 187, 217, 531, 1123, 2360, 2894, 4000
SspDI GGCGCC 1 cut(s) 12
SspI AATATT 4 cut(s) 2614, 2916, 3292, 3703
SspMI CTAG 6 cut(s) 1238, 1596, 2099, 3257, 3333, 3851
SstI GAGCTC 2 cut(s) 3340, 3940
StuI AGGCCT 2 cut(s) 800, 1773
StyD4I CCNGG 7 cut(s) 490, 1836, 1851, 2465, 3232, 3407, 3912
StyI CCWWGG 3 cut(s) 21, 151, 1857
TaiI ACGT 6 cut(s) 458, 1336, 2745, 2828, 2966, 3172
TaqI TCGA 9 cut(s) 398, 404, 2218, 2408, 2434, 2908, 3308, 3367, 3582
TatI WGTACW 2 cut(s) 315, 3833
TauI GCSGC 1 cut(s) 533
TscAI CASTG 7 cut(s) 235, 1784, 2205, 2495, 2863, 3232, 3607
TseFI GTSAC 2 cut(s) 616, 1409
TseI GCWGC 9 cut(s) 161, 881, 1217, 1559, 1624, 2230, 3037, 3132, 3869
Tsp45I GTSAC 2 cut(s) 616, 1409
TspGWI ACGGA 5 cut(s) 287, 1918, 1977, 2420, 3293
TspRI CASTG 7 cut(s) 235, 1784, 2205, 2495, 2863, 3232, 3607
Tth111I GACNNNGTC 1 cut(s) 3785
Van91I CCANNNNNTGG 3 cut(s) 1783, 1858, 2726
VneI GTGCAC 1 cut(s) 2486
VpaK11BI GGWCC 6 cut(s) 4, 731, 2782, 3064, 3230, 3458
XapI RAATTY 7 cut(s) 196, 826, 859, 2595, 2704, 2815, 3549
XceI RCATGY 1 cut(s) 1252
XcmI CCANNNNNNNNNTGG 2 cut(s) 1855, 1864
XmiI GTMKAC 2 cut(s) 933, 2853
XmnI GAANNNNTTC 5 cut(s) 306, 2117, 2420, 2865, 3022
XspI CTAG 6 cut(s) 1238, 1596, 2099, 3257, 3333, 3851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.