FvH4_6g24570

DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005736
Physical Location & Seq
Forward (+)
18632364 .. 18643493
11130 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24570.t6

Sequence Viewer

Length: 4521 bp
ATGGACCACCACCGGCGCCAATCCAAGGATTCTCTCTCCTACTCCAACCTCTTCAACCTTGAGCCTTTGATGAACTTTCAACTTCCACAGCCTGATGATGATTTTGATTATTACGGCAACAGTAGTCAGGATGAGAGCAGAGGTAGCCAAGGTGGAGCTGCTGGTAATGGGATGATGTCCGACCGCGAACTGAGTTCAGTAAAGAAAAGGAGGCGGTCGCAGAATAGTGATTACGAGGAGGATGACAGTTATTACCGTACGCACATTACGGAGGAGAAGTACAGATCAATGCTTGGAGAACACATTCAAAAGTACAAGAGAAGGTTCAAGGATTCGTCATCGAGTCCTGCACCGATGCATATGGGGATTCCAGTTCCCAAGGGGAATAAGGGTTCAAAATCTAGAAAGCTGGCAAATGAGAACCGAGGAGGGTTTTATGAAATGGAAACCACGTCCGAGTGGCTCAATGATGCTATTGCTCAGAAACCAGGGAACTACCACGATGCTGATTTTTCACCTCAAAGTACCGTTGATAGAGTAATATATGAACCTCCTTATCTCGATATTGGAGATGGTTTCACATACAGGATTCCACCAATTTATGATAAACTGGTGACATCTTTACACTTGCCTAGCTTTTCTGATTTTCGGGTGGAGGAAGTTTACTTAAAAGGCACATTGGATTTGGGATCCTTAGCAGAAATGATGGGTAGCGATAAGAAGTTTGGTCCAAAAAATGGGGCAGGAATGGGGGAGCCTTATCCCCTGTATGACTCCCTTCAGGCAAGATTGAAGGCCTTGTCCACTTCTACCTCTGATCAAAATTTCAGCCTCAAAGTTTCTGATATTGGGTTGAATTCCTCCATCCCAGAGGGGGCTGCTGGAAGGATAAAGAGGCTTATTTTGTCCGACGGTGGTGTCTTGCAACCCTACTATGTGAAGGTTTTGGAGAAAGGAGACACATATGAGATCATTGAACGAAGCCTACCCAAGAAGCAAAAGGTAGAGAAAGATCCCTCTCTAATTGAGAAGGAGGAAATGGATAGGATTGGAAGAGTATGGGTAAACATAGTAAGAAGAGACATACCAAAGCATCAGAGATTTTTCACTACTTTTCACCGCAAGCAACTAATTGATGCCAAGAGGGTCTCAGAGAACTGTCAAAGAGAGGTTAAAATGAAGGTGAGTAGATCACTTAAAGTGACAAGGGGTGCTGCAATTCGCACAAGAAAACTAGCCAGGGATATGCTACTGCTTTGGAAGAGAATAGACAAAGAAATGGCAGAAGTGAGGAAGAAAGAGGAAAGAGAAGCTATTGAAATTCGCAAGCGTTTGGAGGAGGAAAGAGAAGCAAAGAGACATGAGCAAAAACTCAATTTTCTCATTCAACAAACTGAACTTTACAGTCACTTTATGCAGAACAAGCCGAGTTTTCAGCCAGCCGGAGACCTGCCTGTAGGAGATGAAAACCAAGATGTATCTCCTAGCTCCTCAGATATTAAGAATATTGAAGAAGATTCTGAGGAAGCTGAACTGAAGAAGGAGGCATTGAAAGCTGCTCAAGATGCAGTTTCAAAGCAGAAAAAGTTAACTAGTGCATTCGACGATGAGTGTCTGAGACTGCGTGAAGCTGCTGAACCTGAGGCACCACAGGATTTTGCTGGAGCTAATAACATAGATCTGCATAACCCATCCACTATGCCCGTCACATCAACTGTTCAGACACCAGAGTTGTTCAAAGGTTCCCTAAAAGAATACCAGCTAAAAGGCCTCCAGTGGCTGGTTAATTGTTACGAGCAGGGTTTAAATGGCATCCTTGCTGATGAGATGGGCCTGGGTAAGACAATTCAGGCCATGGCGTTTTTGGCTCATTTGGCTGAGGAAAAAAATATATGGGGGCCTTTTCTTGTTGTTGCTCCTGCTTCTGTCTTGAACAACTGGGCTGATGAAATTAGTCGTTTCTGCCCTGACTTGAAAACTCTTCCGTATTGGGGTGGTCTTCAAGAGCGTACGGTACTTAGGAAAAAGATCAACGCTAAGAAACTTTACCGTAGGGATGCTGGGTTTCACATTCTCATTACCAGCTACCAGTTGCTAGTGGCTGATGAGAAGTGTTTCCGGCGTGTGAAATGGCAATATATGGTATTAGATGAAGCACAAGCTATCAAAAGTTCAAACAGTATAAGGTGGAAGACACTGCTTAGCTTTAATTGTCGAAATCGTTTGCTGCTTACTGGTACACCTATCCAGAATAATATGGCAGAGTTGTGGGCCCTACTACATTTCATTATGCCTACCTTATTTGACAGTCATGAACAATTCAATGAGTGGTTCTCGAAAGGAATTGAAAACCATGCAGAACATGGGGGAACTTTAAACGAGCACCAGCTTAACAGATTGCATTCCATATTGAAGCCATTCATGCTCCGTCGAGTTAAGACAGATGTTATTTCTGAGCTGACCAGGAAAACTGAAGTTACTGTGCACTGCAAGTTGAGTTCTCGGCAACAAGCTTTTTATCAAGCTATCAAGAACAAGATATCTCTTGCTGAGTTGTTTGACAACAACCGTGGGCATCTTAATGAGAAGAAAATTTTGAACTTGATGAATATTGTCATTCAGCTAAGAAAAGTCTGCAACCATCCTGAATTATTTGAGAGGAATGAAGGAAGCACATATCTTCACTTCGGTGTAATTTCAAATTCTCTTCTTCCTCCTCCATTTGGGGAGTTAGAGGATGTACACTACTCGGGAGGTCAAAATCCTATAACATACCTGGTCCCCAAACTCTTGTATCGAGAAATTCTCCAGAGTTCTGAAACGTTCTGCTCCGCAGTTCGGCACGGGGTCTACATTGAATCTTTTCAGAAACATTTTAACATATATTCACCACAAAATGTGCATCGATCAATATTCTACCAAGAGAATGACTCTGATGAGTTGTCAGTTAGAAGTGGAACGTTTGGTTTTACTCATCTGATGGATCTGTCCCCTGCAGAGGTTGCATTTGTAGGCACTGGTTCTTTTATGGAGCGGCTAATGTTTTCCATTATGAGATGGGACCGGAAATTTTTGGATGGATTGATAGACACACTAATGGAAACTGTGGATGATGATCCTGAATGCAGCTATCTTGAAAGTGGAAAAGTGAGAGCTGTTACACGAATGTTGCTTATGCCTTCAAGATCTATAACTACCGTTTTTCAGAAGAAATTGGCCACCGGTGCTGGTGGTACTCCATTTGAGGGTCTAGTTGTCTCTCATCAGGACAGGCTTCTCTCTAATATTAGGCTTCTCCGTTCGACATACACATTCATCCCCCGTACTAGAGCTCCCCCAGTTAATGCCCATAGTTCAGACCGGAACTTTTCTTACAAAATGAGTGAAGAACAACAGTACCCCTGGGTGAAGAGGTTGTTTTCTGGCTTTGCGCGAACATCCGACTACAATGGACCTAGAAAACCTGACACTCCTCATCATTTAATACAAGAGATTGATTCTGAACTGCCTGTTTCACATTCAGCTCTTCAGTTAACATACAGAATTTTTGGGTCTTGTCCTCCAATGCAAAGCTTTGACCCAGCAAAAATGCTCACTGATTCGGGGAAACTTCAGACACTTGATATATTATTGAAACGCTTGAGGGCAGAAAATCATCGCGTTCTCTTGTTTGCCCAGATGACAAAGATGCTAAATATTCTTGAGGACTACATGAACTACAGGAAATATAGATACCTTAGACTTGATGGGTCCTCCACCATAATGGATCGTAGAGACATGGTTAGAGACTTCCAGCAACGAAATGATATATTTGTTTTCTTGCTGAGTACAAGAGCTGGTGGACTAGGCATTAACTTGACAGCTGCAGATACTGTCATATTTTATGAAAGTGATTGGAATCCAACCCTTGATCTACAGGCAATGGATAGAGCTCATCGGTTGGGTCAAACCAAAGATGTTACTGTCTACCGCCTAATTTGTAAAGAAACGGTTGAGGAGAAGATTCTGCAAAGAGCAAGTCAGAAAAATACTGTCCAGCAGCTTGTAATGATGGGTGGTCATGTTCAGGGTGACCTTTTGGCCCCTGAGGATGTTGTGTCTCTACTTCTTGATGATGCCCAGTTGGAGCAGAAATTAAGAGAAGCTCCGTTACAGGTTAAGGATAAACAAAAGAAGAAACAGACAAAGGGTATACGGGTAGATGCAGAAGGTGATGCCTCTTTGGAAGATTTAACAAACCCTGCTGCATCACAAGGTACTGGAAATGAGGAATCGCCAGATGTCGAAAGATCAAAATCCAATAATAAAAAGAGGAAAACTGTGCCAGACAAGCATACTCCGAGGCCGAAAAATCCTCAAAGTATGGATGAGCCAGAGGGCTATGAATTGGAGGATTCCTTACCAAATACTGATCCACAAGATACAAGACCCAAGAGGCCAAAGAGATCAAAGAAGAGTGTAAATGAGACTCTTGAACCTGCATTTACAGCAGCATCCCCTGTGGTCCCGAGGCAGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBINO Helicase_C SNF2_N

Protein Analysis

1507

Amino Acids

172.41

Weight (kDa)

8.4

Isoelectric Point (pI)

52.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DBINO PF13892 346 - 472 6.5e-43 DNA-binding domain
ResIII PF04851 579 - 746 6e-08 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 583 - 881 2.3e-85 SNF2-related domain
Helicase_C PF00271 1202 - 1312 1.9e-23 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 917
Acc36I ACCTGC 2 cut(s) 1458, 4486
AccB1I GGYRCC 2 cut(s) 15, 1645
AccB7I CCANNNNNTGG 2 cut(s) 737, 1780
AccBSI CCGCTC 1 cut(s) 3034
AccI GTMKAC 3 cut(s) 2850, 3966, 4192
AccII CGCG 3 cut(s) 186, 3433, 3660
AciI CCGC 6 cut(s) 184, 214, 1120, 2832, 3034, 3970
AclI AACGTT 2 cut(s) 2822, 2960
AclWI GGATC 7 cut(s) 684, 697, 1007, 2991, 3110, 3774, 4406
AcoI YGGCCR 1 cut(s) 3216
AcsI RAATTY 8 cut(s) 823, 856, 1320, 2592, 2701, 2802, 3068, 3543
AcuI CTGAAG 5 cut(s) 764, 1556, 2493, 3512, 3596
AcyI GRCGYC 1 cut(s) 16
AfiI CCNNNNNNNGG 9 cut(s) 25, 737, 874, 1780, 1991, 2723, 2838, 2998, 3245
AgeI ACCGGT 1 cut(s) 3221
AhlI ACTAGT 1 cut(s) 1592
AjiI CACGTC 1 cut(s) 453
AjnI CCWGG 6 cut(s) 487, 1238, 1833, 2462, 2775, 3401
AleI CACNNNNGTG 1 cut(s) 2688
AloI GAACNNNNNNTCC 2 cut(s) 376, 408
Alw21I GWGCWC 4 cut(s) 2385, 2487, 3334, 3934
Alw44I GTGCAC 1 cut(s) 2483
AlwI GGATC 7 cut(s) 684, 697, 1007, 2991, 3110, 3774, 4406
AlwNI CAGNNNCTG 2 cut(s) 1780, 3872
Ama87I CYCGRG 2 cut(s) 2749, 4507
ApaI GGGCCC 1 cut(s) 2275
ApaLI GTGCAC 1 cut(s) 2483
ApoI RAATTY 8 cut(s) 823, 856, 1320, 2592, 2701, 2802, 3068, 3543
ArsI GACNNNNNNTTYG 2 cut(s) 4003, 4035
AsiGI ACCGGT 1 cut(s) 3221
Asp700I GAANNNNTTC 4 cut(s) 303, 2114, 2417, 2862
AspLEI GCGC 2 cut(s) 18, 3433
AsuHPI GGTGA 8 cut(s) 507, 625, 1109, 1195, 2880, 3418, 4082, 4223
AvaI CYCGRG 2 cut(s) 2749, 4507
AvaII GGWCC 7 cut(s) 4, 728, 2779, 3061, 3452, 3750, 4504
AxyI CCTNAGG 2 cut(s) 1641, 4086
BaeGI GKGCMC 2 cut(s) 2275, 2487
BaeI ACNNNNGTAYC 4 cut(s) 2777, 2810, 3380, 3413
BalI TGGCCA 1 cut(s) 3218
BamHI GGATCC 1 cut(s) 689
BanI GGYRCC 2 cut(s) 15, 1645
BanII GRGCYC 3 cut(s) 2275, 3334, 3934
BarI GAAGNNNNNNTAC 2 cut(s) 4134, 4166
BbsI GAAGAC 2 cut(s) 1991, 2198
Bbv12I GWGCWC 4 cut(s) 2385, 2487, 3334, 3934
BbvCI CCTCAGC 1 cut(s) 1878
BceAI ACGGC 1 cut(s) 130
BciT130I CCWGG 6 cut(s) 489, 1240, 1835, 2464, 2777, 3403
BclI TGATCA 1 cut(s) 817
BcuI ACTAGT 1 cut(s) 1592
BfmI CTRYAG 5 cut(s) 1455, 2994, 3718, 3864, 3914
BfoI RGCGCY 1 cut(s) 19
BfuAI ACCTGC 2 cut(s) 1458, 4486
BglII AGATCT 2 cut(s) 1678, 3185
BlpI GCTNAGC 1 cut(s) 2201
Bme1390I CCNGG 6 cut(s) 489, 1240, 1835, 2464, 2777, 3403
Bme18I GGWCC 7 cut(s) 4, 728, 2779, 3061, 3452, 3750, 4504
BmeT110I CYCGRG 2 cut(s) 2749, 4507
BmgBI CACGTC 1 cut(s) 453
BmrFI CCNGG 6 cut(s) 489, 1240, 1835, 2464, 2777, 3403
BmrI ACTGGG 3 cut(s) 1948, 3332, 4114
BmuI ACTGGG 3 cut(s) 1948, 3332, 4114
BpiI GAAGAC 2 cut(s) 1991, 2198
BplI GAGNNNNNCTC 6 cut(s) 2318, 2350, 2790, 2822, 2915, 2947
BpmI CTGGAG 3 cut(s) 1683, 1757, 2792
Bpu10I CCTNAGC 2 cut(s) 694, 1878
Bpu1102I GCTNAGC 1 cut(s) 2201
BpuEI CTTGAG 4 cut(s) 80, 1545, 3661, 3722
Bsa29I ATCGAT 1 cut(s) 2905
BsaBI GATNNNNATC 3 cut(s) 3114, 3897, 4294
BsaHI GRCGYC 1 cut(s) 16
BsaI GGTCTC 2 cut(s) 1153, 1440
BsaWI WCCGGW 3 cut(s) 3063, 3221, 3360
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 3316, 3346
Bsc4I CCNNNNNNNGG 9 cut(s) 25, 737, 874, 1780, 1991, 2723, 2838, 2998, 3245
Bse118I RCCGGY 2 cut(s) 12, 3221
Bse21I CCTNAGG 2 cut(s) 1641, 4086
Bse3DI GCAATG 1 cut(s) 3927
Bse8I GATNNNNATC 3 cut(s) 3114, 3897, 4294
BseBI CCWGG 6 cut(s) 489, 1240, 1835, 2464, 2777, 3403
BseCI ATCGAT 1 cut(s) 2905
BseJI GATNNNNATC 3 cut(s) 3114, 3897, 4294
BseLI CCNNNNNNNGG 9 cut(s) 25, 737, 874, 1780, 1991, 2723, 2838, 2998, 3245
BseMI GCAATG 1 cut(s) 3927
BseRI GAGGAG 8 cut(s) 251, 287, 441, 1352, 1480, 2706, 3462, 4010
BseSI GKGCMC 2 cut(s) 2275, 2487
BseYI CCCAGC 2 cut(s) 2060, 3580
BsgI GTGCAG 1 cut(s) 333
Bsh1236I CGCG 3 cut(s) 186, 3433, 3660
Bsh1285I CGRYCG 2 cut(s) 184, 218
BshNI GGYRCC 2 cut(s) 15, 1645
BshTI ACCGGT 1 cut(s) 3221
BshVI ATCGAT 1 cut(s) 2905
BsiEI CGRYCG 2 cut(s) 184, 218
BsiHKAI GWGCWC 4 cut(s) 2385, 2487, 3334, 3934
BsiHKCI CYCGRG 2 cut(s) 2749, 4507
BsiSI CCGG 6 cut(s) 13, 1443, 2119, 3064, 3222, 3361
BsiWI CGTACG 2 cut(s) 257, 2009
BslFI GGGAC 4 cut(s) 2765, 2974, 3074, 4490
BslI CCNNNNNNNGG 9 cut(s) 25, 737, 874, 1780, 1991, 2723, 2838, 2998, 3245
BsmFI GGGAC 4 cut(s) 2765, 2974, 3074, 4490
BsmI GAATGC 3 cut(s) 1598, 2401, 3128
Bso31I GGTCTC 2 cut(s) 1153, 1440
BsoBI CYCGRG 2 cut(s) 2749, 4507
Bsp120I GGGCCC 1 cut(s) 2271
Bsp1286I GDGCHC 5 cut(s) 2275, 2385, 2487, 3334, 3934
Bsp1407I TGTACA 1 cut(s) 2740
Bsp1720I GCTNAGC 1 cut(s) 2201
Bsp19I CCATGG 1 cut(s) 1854
BspACI CCGC 6 cut(s) 184, 214, 1120, 2832, 3034, 3970
BspDI ATCGAT 1 cut(s) 2905
BspFNI CGCG 3 cut(s) 186, 3433, 3660
BspHI TCATGA 1 cut(s) 2311
BspMAI CTGCAG 2 cut(s) 2998, 3868
BspMI ACCTGC 2 cut(s) 1458, 4486
BspPI GGATC 7 cut(s) 684, 697, 1007, 2991, 3110, 3774, 4406
BspQI GCTCTTC 1 cut(s) 3531
BspT107I GGYRCC 2 cut(s) 15, 1645
BspTNI GGTCTC 2 cut(s) 1153, 1440
BsrBI CCGCTC 1 cut(s) 3034
BsrDI GCAATG 1 cut(s) 3927
BsrFI RCCGGY 2 cut(s) 12, 3221
BsrGI TGTACA 1 cut(s) 2740
BssAI RCCGGY 2 cut(s) 12, 3221
BssNAI GTATAC 1 cut(s) 4193
BssNI GRCGYC 1 cut(s) 16
BssT1I CCWWGG 4 cut(s) 24, 148, 378, 1854
Bst1107I GTATAC 1 cut(s) 4193
Bst2UI CCWGG 6 cut(s) 489, 1240, 1835, 2464, 2777, 3403
Bst6I CTCTTC 9 cut(s) 56, 1048, 1072, 1256, 1986, 2712, 3404, 3531, 4448
BstACI GRCGYC 1 cut(s) 16
BstAPI GCANNNNNTGC 1 cut(s) 3002
BstAUI TGTACA 1 cut(s) 2740
BstC8I GCNNGC 4 cut(s) 411, 1124, 1328, 1440
BstDSI CCRYGG 2 cut(s) 1854, 2569
BstEII GGTNACC 1 cut(s) 4070
BstFNI CGCG 3 cut(s) 186, 3433, 3660
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 2 cut(s) 18, 3433
BstMCI CGRYCG 2 cut(s) 184, 218
BstNI CCWGG 6 cut(s) 489, 1240, 1835, 2464, 2777, 3403
BstPI GGTNACC 1 cut(s) 4070
BstSCI CCNGG 6 cut(s) 487, 1238, 1833, 2462, 2775, 3401
BstSFI CTRYAG 5 cut(s) 1455, 2994, 3718, 3864, 3914
BstSLI GKGCMC 2 cut(s) 2275, 2487
BstUI CGCG 3 cut(s) 186, 3433, 3660
BstV2I GAAGAC 2 cut(s) 1991, 2198
BstX2I RGATCY 5 cut(s) 689, 1012, 1678, 2983, 3185
BstXI CCANNNNNNTGG 1 cut(s) 3763
BstYI RGATCY 5 cut(s) 689, 1012, 1678, 2983, 3185
BstZ17I GTATAC 1 cut(s) 4193
Bsu15I ATCGAT 1 cut(s) 2905
Bsu36I CCTNAGG 2 cut(s) 1641, 4086
BsuTUI ATCGAT 1 cut(s) 2905
BtgI CCRYGG 2 cut(s) 1854, 2569
BtgZI GCGATG 1 cut(s) 3641
BtrI CACGTC 1 cut(s) 453
BtsI GCAGTG 2 cut(s) 2195, 2485
BtsIMutI CAGTG 5 cut(s) 1781, 2195, 2485, 3015, 3594
BveI ACCTGC 2 cut(s) 1458, 4486
Cac8I GCNNGC 4 cut(s) 411, 1124, 1328, 1440
CaiI CAGNNNCTG 2 cut(s) 1780, 3872
CciI TCATGA 1 cut(s) 2311
CfoI GCGC 2 cut(s) 18, 3433
Cfr10I RCCGGY 2 cut(s) 12, 3221
ClaI ATCGAT 1 cut(s) 2905
CsiI ACCWGGT 1 cut(s) 2775
CspAI ACCGGT 1 cut(s) 3221
CspCI CAANNNNNGTGG 2 cut(s) 2551, 2586
CviAII CATG 9 cut(s) 1361, 1855, 2312, 2354, 2363, 2422, 3712, 3778, 4061
DinI GGCGCC 1 cut(s) 17
DraI TTTAAA 2 cut(s) 1806, 2376
DrdI GACNNNNNNGTC 1 cut(s) 917
DseDI GACNNNNNNGTC 1 cut(s) 917
EaeI YGGCCR 1 cut(s) 3216
Eam1104I CTCTTC 9 cut(s) 56, 1048, 1072, 1256, 1986, 2712, 3404, 3531, 4448
EarI CTCTTC 9 cut(s) 56, 1048, 1072, 1256, 1986, 2712, 3404, 3531, 4448
Ecl136II GAGCTC 2 cut(s) 3332, 3932
Eco130I CCWWGG 4 cut(s) 24, 148, 378, 1854
Eco147I AGGCCT 2 cut(s) 797, 1770
Eco24I GRGCYC 3 cut(s) 2275, 3334, 3934
Eco31I GGTCTC 2 cut(s) 1153, 1440
Eco32I GATATC 1 cut(s) 2541
Eco47I GGWCC 7 cut(s) 4, 728, 2779, 3061, 3452, 3750, 4504
Eco53kI GAGCTC 2 cut(s) 3332, 3932
Eco57I CTGAAG 5 cut(s) 764, 1556, 2493, 3512, 3596
Eco81I CCTNAGG 2 cut(s) 1641, 4086
Eco88I CYCGRG 2 cut(s) 2749, 4507
Eco91I GGTNACC 1 cut(s) 4070
EcoICRI GAGCTC 2 cut(s) 3332, 3932
EcoO109I RGGNCCY 3 cut(s) 1898, 2272, 3750
EcoO65I GGTNACC 1 cut(s) 4070
EcoRI GAATTC 1 cut(s) 856
EcoRII CCWGG 6 cut(s) 487, 1238, 1833, 2462, 2775, 3401
EcoRV GATATC 1 cut(s) 2541
EcoT14I CCWWGG 4 cut(s) 24, 148, 378, 1854
EcoT22I ATGCAT 1 cut(s) 360
EcoT38I GRGCYC 3 cut(s) 2275, 3334, 3934
EgeI GGCGCC 1 cut(s) 17
EheI GGCGCC 1 cut(s) 17
ErhI CCWWGG 4 cut(s) 24, 148, 378, 1854
FaeI CATG 9 cut(s) 1364, 1858, 2315, 2357, 2366, 2425, 3715, 3781, 4064
FaqI GGGAC 4 cut(s) 2765, 2974, 3074, 4490
FatI CATG 9 cut(s) 1360, 1854, 2311, 2353, 2362, 2421, 3711, 3777, 4060
FauNDI CATATG 2 cut(s) 360, 964
FbaI TGATCA 1 cut(s) 817
FblI GTMKAC 3 cut(s) 2850, 3966, 4192
FriOI GRGCYC 3 cut(s) 2275, 3334, 3934
GlaI GCGC 2 cut(s) 17, 3432
GsaI CCCAGC 2 cut(s) 2064, 3584
GsuI CTGGAG 3 cut(s) 1683, 1757, 2792
HaeII RGCGCY 1 cut(s) 19
HapII CCGG 6 cut(s) 13, 1443, 2119, 3064, 3222, 3361
HhaI GCGC 2 cut(s) 18, 3433
Hin1I GRCGYC 1 cut(s) 16
Hin1II CATG 9 cut(s) 1364, 1858, 2315, 2357, 2366, 2425, 3715, 3781, 4064
Hin6I GCGC 2 cut(s) 16, 3431
HinP1I GCGC 2 cut(s) 16, 3431
HincII GTYRAC 2 cut(s) 1590, 3534
HindII GTYRAC 2 cut(s) 1590, 3534
HindIII AAGCTT 2 cut(s) 2511, 3571
HpaI GTTAAC 2 cut(s) 1590, 3534
HpaII CCGG 6 cut(s) 13, 1443, 2119, 3064, 3222, 3361
HphI GGTGA 8 cut(s) 507, 625, 1109, 1195, 2880, 3418, 4082, 4223
Hpy99I CGWCG 3 cut(s) 914, 1607, 2433
HpyCH4IV ACGT 3 cut(s) 452, 2822, 2960
HpySE526I ACGT 3 cut(s) 452, 2822, 2960
Hsp92I GRCGYC 1 cut(s) 16
Hsp92II CATG 9 cut(s) 1364, 1858, 2315, 2357, 2366, 2425, 3715, 3781, 4064
HspAI GCGC 2 cut(s) 16, 3431
KasI GGCGCC 1 cut(s) 15
Ksp22I TGATCA 1 cut(s) 817
KspAI GTTAAC 2 cut(s) 1590, 3534
LguI GCTCTTC 1 cut(s) 3531
MabI ACCWGGT 1 cut(s) 2775
MaeII ACGT 3 cut(s) 452, 2822, 2960
MbiI CCGCTC 1 cut(s) 3034
MflI RGATCY 5 cut(s) 689, 1012, 1678, 2983, 3185
MhlI GDGCHC 5 cut(s) 2275, 2385, 2487, 3334, 3934
MlsI TGGCCA 1 cut(s) 3218
MluNI TGGCCA 1 cut(s) 3218
Mly113I GGCGCC 1 cut(s) 16
MlyI GAGTC 4 cut(s) 352, 767, 2924, 4462
MmeI TCCRAC 6 cut(s) 69, 204, 933, 3465, 3926, 4104
Mox20I TGGCCA 1 cut(s) 3218
Mph1103I ATGCAT 1 cut(s) 360
MroXI GAANNNNTTC 4 cut(s) 303, 2114, 2417, 2862
MscI TGGCCA 1 cut(s) 3218
MslI CAYNNNNRTG 4 cut(s) 2580, 2688, 3095, 3761
Msp20I TGGCCA 1 cut(s) 3218
MspA1I CMGCKG 1 cut(s) 3863
MspI CCGG 6 cut(s) 13, 1443, 2119, 3064, 3222, 3361
MspR9I CCNGG 6 cut(s) 489, 1240, 1835, 2464, 2777, 3403
Mva1269I GAATGC 3 cut(s) 1598, 2401, 3128
MvaI CCWGG 6 cut(s) 489, 1240, 1835, 2464, 2777, 3403
MvnI CGCG 3 cut(s) 186, 3433, 3660
NarI GGCGCC 1 cut(s) 16
NcoI CCATGG 1 cut(s) 1854
NdeI CATATG 2 cut(s) 360, 964
NlaIII CATG 9 cut(s) 1364, 1858, 2315, 2357, 2366, 2425, 3715, 3781, 4064
NmeAIII GCCGAG 2 cut(s) 1452, 2482
NmuCI GTSAC 5 cut(s) 613, 1201, 1406, 1705, 4070
NsiI ATGCAT 1 cut(s) 360
OliI CACNNNNGTG 1 cut(s) 2688
PagI TCATGA 1 cut(s) 2311
PasI CCCWGGG 1 cut(s) 3402
PceI AGGCCT 2 cut(s) 797, 1770
PciSI GCTCTTC 1 cut(s) 3531
PctI GAATGC 3 cut(s) 1598, 2401, 3128
PdmI GAANNNNTTC 4 cut(s) 303, 2114, 2417, 2862
Pfl23II CGTACG 2 cut(s) 257, 2009
PflMI CCANNNNNTGG 2 cut(s) 737, 1780
PinAI ACCGGT 1 cut(s) 3221
PleI GAGTC 4 cut(s) 351, 767, 2924, 4462
PluTI GGCGCC 1 cut(s) 19
PpsI GAGTC 4 cut(s) 351, 767, 2924, 4462
PpuMI RGGWCCY 1 cut(s) 3750
Psp124BI GAGCTC 2 cut(s) 3334, 3934
Psp1406I AACGTT 2 cut(s) 2822, 2960
Psp5II RGGWCCY 1 cut(s) 3750
Psp6I CCWGG 6 cut(s) 487, 1238, 1833, 2462, 2775, 3401
PspEI GGTNACC 1 cut(s) 4070
PspFI CCCAGC 2 cut(s) 2060, 3580
PspGI CCWGG 6 cut(s) 487, 1238, 1833, 2462, 2775, 3401
PspLI CGTACG 2 cut(s) 257, 2009
PspOMI GGGCCC 1 cut(s) 2271
PspPPI RGGWCCY 1 cut(s) 3750
PsrI GAACNNNNNNTAC 4 cut(s) 969, 1001, 3356, 3388
PstI CTGCAG 2 cut(s) 2998, 3868
PstNI CAGNNNCTG 2 cut(s) 1780, 3872
PsuI RGATCY 5 cut(s) 689, 1012, 1678, 2983, 3185
PvuII CAGCTG 1 cut(s) 3863
RseI CAYNNNNRTG 4 cut(s) 2580, 2688, 3095, 3761
SacI GAGCTC 2 cut(s) 3334, 3934
SapI GCTCTTC 1 cut(s) 3531
SchI GAGTC 4 cut(s) 352, 767, 2924, 4462
ScrFI CCNGG 6 cut(s) 489, 1240, 1835, 2464, 2777, 3403
SduI GDGCHC 5 cut(s) 2275, 2385, 2487, 3334, 3934
SexAI ACCWGGT 1 cut(s) 2775
SfcI CTRYAG 5 cut(s) 1455, 2994, 3718, 3864, 3914
SfoI GGCGCC 1 cut(s) 17
SgrAI CRCCGGYG 2 cut(s) 12, 3221
SinI GGWCC 7 cut(s) 4, 728, 2779, 3061, 3452, 3750, 4504
SmiMI CAYNNNNRTG 4 cut(s) 2580, 2688, 3095, 3761
SmlI CTYRAG 4 cut(s) 59, 1560, 3640, 3701
SmoI CTYRAG 4 cut(s) 59, 1560, 3640, 3701
SpeI ACTAGT 1 cut(s) 1592
SseBI AGGCCT 2 cut(s) 797, 1770
SsiI CCGC 6 cut(s) 184, 214, 1120, 2832, 3034, 3970
SspDI GGCGCC 1 cut(s) 15
SspI AATATT 5 cut(s) 1507, 2611, 2913, 3286, 3697
SstI GAGCTC 2 cut(s) 3334, 3934
StuI AGGCCT 2 cut(s) 797, 1770
StyD4I CCNGG 6 cut(s) 487, 1238, 1833, 2462, 2775, 3401
StyI CCWWGG 4 cut(s) 24, 148, 378, 1854
TaiI ACGT 3 cut(s) 455, 2825, 2963
TatI WGTACW 4 cut(s) 279, 312, 2740, 3827
TauI GCSGC 1 cut(s) 3037
TscAI CASTG 5 cut(s) 1781, 2202, 2492, 3022, 3601
TseFI GTSAC 5 cut(s) 613, 1201, 1406, 1705, 4070
Tsp45I GTSAC 5 cut(s) 613, 1201, 1406, 1705, 4070
TspGWI ACGGA 5 cut(s) 284, 1974, 2417, 3287, 4137
TspRI CASTG 5 cut(s) 1781, 2202, 2492, 3022, 3601
Van91I CCANNNNNTGG 2 cut(s) 737, 1780
VneI GTGCAC 1 cut(s) 2483
VpaK11BI GGWCC 7 cut(s) 4, 728, 2779, 3061, 3452, 3750, 4504
XapI RAATTY 8 cut(s) 823, 856, 1320, 2592, 2701, 2802, 3068, 3543
XbaI TCTAGA 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 2 cut(s) 1861, 2360
XmiI GTMKAC 3 cut(s) 2850, 3966, 4192
XmnI GAANNNNTTC 4 cut(s) 303, 2114, 2417, 2862
Zsp2I ATGCAT 1 cut(s) 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.