Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002698

Physical Location & Seq

Genomic Coordinates Forward (+)

22298373 .. 22303718

Gene Structure 5346 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G60330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2886 bp

ATGACGGACATAGAAGCTCTCAAGGCTATCACCACGGAGAGCATTGATCTGGAAAATGTTCCCGTTGAAGAGGTTTTTCAACATCTCAAATGCACGAAAGAAGGATTGACCTCCAATGAAGTGCAGGAGCGACTTACCTTGTTTGGTTACAACAAACTTGAGGAGAAAAAGGAGAGTAAGATACTCAAGTTCTTGGGATTCATGTGGAATCCACTTTCATGGGTCATGGAAGCTGCAGCCCTCATGGCCATTGGTCTTGCACATGGAGGAGGAAAGCCAGCGGATTATCACGATTTCGTGGGTATTGTGGTCTTACTTTTGATAAACTCGACAATCAGTTTTGTAGAAGAAAACAATGCTGGAAATGCAGCTGCTGCTCTCATGGCTCAGTTAGCCCCTAAAGCGAAGGCCGTCCGCGATGGCAAATGGAATGAGATAGATGCAGCTGAGTTGGTTCCAGGAGATATAGTTAGTATCAAACTTGGAGATATCATTCCTGCTGATGCTCGTCTTCTTGAAGGAGACCCTTTAAAGATTGACCAGGCAACACTTACAGGTGAATCTCTTCCGGTAACCAAAAATCCTGGTGCTTCAGTGTACTCTGGTTCAACTTGCAAGCAAGGTGAAATTGAAGCAGTTGTTATAGCGACTGGTGTCCACACTTTCTTTGGAAAGGCGGCTCATCTTGTGGATAGTACTACGCATGTCGGTCACTTCCAGAAGGTTTTGACAGCAATAGGAAATTTCTGTATCTGCTCGATTGCAGTAGGAATGGCAATTGAAATCGTTGTTATATACGGTCTTCAAAAGAGAGGGTACCGTGTTGGTATTGATAACCTTCTTGTTTTATTGATTGGTGGGATCCCCATTGCAATGCCTACTGTTCTCTCTGTTACAATGGCAATTGGTGCTCATCGCTTGGCTCAACAGGGTGCCATAACAAAGAGAATGACAGCCATTGAAGAAATGGCAGGAATGGATGTTCTGTGCAGTGATAAAACAGGTACTCTCACGCTCAACAAGCTTTCTGTGGATAAGAATCTGATCGAGGTTTTTAAGAGGGGCATCGACAGAGATATGGCTGTGCTTATGGCTGCAAGAGCTGCACGTTTAGAGAACCAAGATGCTATTGATACTGCAATTGTTTCGATGTTGTCAGACCCGAAAGAGGCACGTGCCGGGATTAAAGAACTCCATTTTCTTCCATTCAGTCCAGCTAATAGAAGAACTGCACTAACGTACCTAGACGGAGAAGGCAAAATGCACCGAGTGAGCAAAGGCGCGCCTGAAGAGATTTTGGATATGGCCCACAACAAATTAGAAATCAAGGAGAAAGTACATGCTACAATCGACAAATTTGCAGAACGTGGCCTAAGATCGCTTGGGTTGGCATATCAGGAAGTACCAGATGGTGATGTTAAAGGTGAAGGTGGTCCATGGGACTTTGTAGCTCTTCTTCCTCTGTTTGATCCTCCTCGTCATGACAGTGCACAGACAATTGAGCGAGCACTTCATCTTGGAGTCAGTGTTAAAATGATCACAGGTGACCAGCTTGCAATTGCCAAAGAAACAGGAAGAAGGCTTGGAATGGGCACAAACATGTACCCATCTTCATCTTTGCTTTCTGATAACAACACTGAAGGAGTTTCTGTCGATGAACTTATCGAGAACGCAGATGGTTTTGCAGGAGTCTTTCCTGAGCATAAGTATGAGATTGTGAAGAGATTACAATCCCGAAAACACATATGTGGTATGACTGGTGATGGAGTGAATGACGCACCTGCCTTGAAGAAAGCTGATATTGGAATTGCTGTGGATGATGCCACTGATGCTGCTCGGGGTGCTTCTGATATTGTCTTAACAGAACCTGGACTTAGTGTTATCATCAGTGCTGTCTTGACTAGCCGTGCAATTTTCCAGAGAATGAAAAATTACACGATATATGCTGTTTCTATCACTATACGTATTGTCATGGGTTTCATGCTTCTATGTGTCTTCTGGGAGTTTGACTTCCCTCCATTCATGGTTCTTGTAATTGCAATCCTTAACGATGGTACTATAATGACCATATCCAAGGACAGAGTCAAGCCTTCTCCAACTCCAGATTGTTGGAAACTCAAAGAAATTTTTGCTACTGGTGTTGTGCTTGGAGCTTACTTGGCGATCATGACTGTTGTGTTTTTCTGGGCAGCATACGAAACCAACTTCTTCCATAACATTTTCCATGTGAGAAACTTCAACCAGCACCATTTCAAAATGAAAGACAAGAAAGTGGCTGCACATTTGAATGAACAGATGGCTTCCGCGGTCTACCTTCAAGTCAGCACGATCAGCCAAGCGTTGATCTTCGTGACCCGTTCCAGGAGCTGGTCATTTGTTGAGCGTCCCGGTTTTCTTCTTGTAATTGCTTTCCTAATAGCTCAGCTGGTTGCATCGGTGATATCGGCAATGGCAAATTGGCCTTTTGCTGGAATCAGAAGCATTGGATGGGGTTGGACTGGAGTTATCTGGATATTCAACATAGTAACTTACATGTTACTTGATCCTATCAAATTCCTAGTCCGTTATGCTCTAAGTGGCAAATCATGGGACCGAATGGTCGAGGGGAGGACTGCACTCACAGGAAAGAAGAATTTTGGCCAAGAAGAAAGAATGGCTGCGTGGGCCACCGAGAAGCGTACACAGCACGGTTTAGAGACGGGTCAAAAGCCAGTTTATGAGAGAAATAGCGCAACGGAACTTAACAATATGGCTGAGGAAGCTAAAAGGCGTGCAGAAATTGCAAGAATGAGAGAGCTTCAGACATTGAAGGGGAAGGTTGAATCAGCTGCAAAGCTGAAAGGCTATGATCTTGAGGATCCTAACAGCAACAACTACACAATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

961

Amino Acids

105.52

Weight (kDa)

6.38

Isoelectric Point (pI)

32.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	19 - 82	1.8e-15	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	131 - 230	4.7e-32	P-type ATPase actuator domain
Hydrolase	PF00702	326 - 600	6.6e-12	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002698)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60330 AT3G60330 AT3G60330 AT3G60330
fragaria_vesca	FvH4_5g19790 FvH4_7g09980
malus_domestica	MD02G1216000.v1.1 MD03G1197700.v1.1 MD03G1197800.v1.1 MD04G1058300.v1.1
prunus_persica	Prupe.2G128800_v2.0.a1 Prupe.5G062200_v2.0.a1
pyrus_communis	pycom04g05210
rosa_chinensis	RchiOBHm_Chr1g0347581 RchiOBHm_Chr4g0412601 RchiOBHm_Chr7g0204571
rosa_laevigata	RLG00000003448 RLG00000028736 RLG00000030415
rosa_multiflora	Rmu_sc0001211.1_g000115 Rmu_sc0005397.1_g000001 Rmu_sc0007327.1_g000001 Rmu_sc0021384.1_g000002 Rmu_sc0024460.1_g000001 Rmu_sc0027638.1_g000006
rosa_roxburghii	Rroxscaffold_3G00252980 Rroxscaffold_4G00307640 Rroxscaffold_4G00307710
rosa_rugosa	Rorug01G0188000 Rorug07G0085200
rosa_samantha	Rh1DG200900 Rh7AG214600 Rh7BG212100 Rh7CG227700 Rh7DG222300
rosa_wichuraiana	Rw0G013650 Rw0G018200 Rw1G017220 Rw7G018620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1789
AasI	GACNNNNNNGTC	1 cut(s)	2597
Acc36I	ACCTGC	1 cut(s)	1789
Acc65I	GGTACC	1 cut(s)	816
AccB1I	GGYRCC	2 cut(s)	816, 932
AccB7I	CCANNNNNTGG	1 cut(s)	2367
AccI	GTMKAC	1 cut(s)	2310
AccII	CGCG	3 cut(s)	417, 1283, 2306
AciI	CCGC	5 cut(s)	281, 415, 677, 2304, 2306
AclWI	GGATC	6 cut(s)	856, 869, 1463, 2537, 2852, 2865
AcoI	YGGCCR	2 cut(s)	246, 2638
AcsI	RAATTY	5 cut(s)	742, 1355, 2124, 2552, 2632
AcuI	CTGAAG	4 cut(s)	576, 1308, 1659, 2783
AcvI	CACGTG	1 cut(s)	1175
AdeI	CACNNNGTG	1 cut(s)	1270
AfiI	CCNNNNNNNGG	4 cut(s)	1168, 2361, 2367, 2468
AflIII	ACRYGT	2 cut(s)	1599, 2532
AjnI	CCWGG	5 cut(s)	457, 540, 583, 1867, 2360
AleI	CACNNNNGTG	1 cut(s)	1746
AloI	GAACNNNNNNTCC	2 cut(s)	966, 998
Alw21I	GWGCWC	3 cut(s)	913, 1492, 1510
Alw26I	GTCTC	2 cut(s)	516, 2690
Alw44I	GTGCAC	1 cut(s)	1488
AlwI	GGATC	6 cut(s)	856, 869, 1463, 2537, 2852, 2865
AlwNI	CAGNNNCTG	3 cut(s)	374, 1868, 2367
Ama87I	CYCGRG	1 cut(s)	1836
AoxI	GGCC	7 cut(s)	246, 408, 1305, 1369, 2459, 2638, 2664
ApaLI	GTGCAC	1 cut(s)	1488
ApoI	RAATTY	5 cut(s)	742, 1355, 2124, 2552, 2632
ArsI	GACNNNNNNTTYG	2 cut(s)	2544, 2576
AscI	GGCGCGCC	1 cut(s)	1281
Asp700I	GAANNNNTTC	2 cut(s)	57, 564
Asp718I	GGTACC	1 cut(s)	816
AspLEI	GCGC	3 cut(s)	1283, 1285, 2732
AspS9I	GGNCC	4 cut(s)	1306, 1433, 2590, 2664
AsuC2I	CCSGG	2 cut(s)	1180, 2388
AsuHPI	GGTGA	8 cut(s)	22, 569, 635, 1424, 1436, 1556, 1772, 2449
AvaI	CYCGRG	1 cut(s)	1836
AvaII	GGWCC	2 cut(s)	1433, 2590
BaeGI	GKGCMC	2 cut(s)	1492, 1595
BaeI	ACNNNNGTAYC	4 cut(s)	173, 206, 1223, 1256
BalI	TGGCCA	2 cut(s)	248, 2640
BamHI	GGATCC	2 cut(s)	861, 2857
BanI	GGYRCC	2 cut(s)	816, 932
BarI	GAAGNNNNNNTAC	2 cut(s)	1440, 1472
BbrPI	CACGTG	1 cut(s)	1175
BbsI	GAAGAC	3 cut(s)	503, 794, 1987
Bbv12I	GWGCWC	3 cut(s)	913, 1492, 1510
BbvCI	CCTCAGC	1 cut(s)	2754
BccI	CCATC	8 cut(s)	413, 1403, 1615, 1670, 1757, 2045, 2290, 2481
BceAI	ACGGC	2 cut(s)	395, 1890
BcgI	CGANNNNNNTGC	4 cut(s)	1128, 1162, 1340, 1374
BciT130I	CCWGG	5 cut(s)	459, 542, 585, 1869, 2362
BclI	TGATCA	1 cut(s)	1536
BcnI	CCSGG	2 cut(s)	1180, 2388
BcoDI	GTCTC	2 cut(s)	516, 2690
BfaI	CTAG	3 cut(s)	1244, 1902, 2558
BfmI	CTRYAG	1 cut(s)	234
BfuAI	ACCTGC	1 cut(s)	1789
BglI	GCCNNNNNGGC	1 cut(s)	245
BlpI	GCTNAGC	1 cut(s)	2421
BmcAI	AGTACT	1 cut(s)	697
Bme1390I	CCNGG	7 cut(s)	459, 542, 585, 1180, 1869, 2362, 2388
Bme18I	GGWCC	2 cut(s)	1433, 2590
BmeT110I	CYCGRG	1 cut(s)	1836
BmgT120I	GGNCC	4 cut(s)	1306, 1433, 2590, 2664
BmiI	GGNNCC	6 cut(s)	456, 818, 863, 934, 2591, 2859
BmrFI	CCNGG	7 cut(s)	459, 542, 585, 1180, 1869, 2362, 2388
BmsI	GCATC	7 cut(s)	430, 493, 1074, 1114, 1810, 1819, 2441
BoxI	GACNNNNGTC	1 cut(s)	653
BpiI	GAAGAC	3 cut(s)	503, 794, 1987
BpmI	CTGGAG	2 cut(s)	2085, 2520
Bpu10I	CCTNAGC	2 cut(s)	1698, 2754
Bpu1102I	GCTNAGC	1 cut(s)	2421
BpuEI	CTTGAG	4 cut(s)	5, 170, 179, 2873
BpuMI	CCSGG	2 cut(s)	1180, 2388
BsaAI	YACGTR	2 cut(s)	1175, 1964
BsaBI	GATNNNNATC	2 cut(s)	1038, 1729
BsaI	GGTCTC	1 cut(s)	516
BsaJI	CCNNGG	4 cut(s)	33, 1436, 2073, 2304
BsaWI	WCCGGW	1 cut(s)	568
BsaXI	ACNNNNNCTCC	6 cut(s)	453, 483, 1512, 1542, 1635, 1665
Bsc4I	CCNNNNNNNGG	4 cut(s)	1168, 2361, 2367, 2468
Bse1I	ACTGG	5 cut(s)	655, 1762, 2140, 2503, 2711
Bse3DI	GCAATG	3 cut(s)	867, 879, 2454
Bse8I	GATNNNNATC	2 cut(s)	1038, 1729
BseBI	CCWGG	5 cut(s)	459, 542, 585, 1869, 2362
BseDI	CCNNGG	4 cut(s)	33, 1436, 2073, 2304
BseGI	GGATG	3 cut(s)	985, 1822, 2492
BseJI	GATNNNNATC	2 cut(s)	1038, 1729
BseLI	CCNNNNNNNGG	4 cut(s)	1168, 2361, 2367, 2468
BseMI	GCAATG	3 cut(s)	867, 879, 2454
BseMII	CTCAG	5 cut(s)	401, 438, 1689, 2435, 2745
BseNI	ACTGG	5 cut(s)	655, 1762, 2140, 2503, 2711
BsePI	GCGCGC	1 cut(s)	1281
BseRI	GAGGAG	3 cut(s)	176, 282, 1464
BseSI	GKGCMC	2 cut(s)	1492, 1595
BsgI	GTGCAG	7 cut(s)	143, 1009, 1089, 1215, 2262, 2598, 2793
Bsh1236I	CGCG	3 cut(s)	417, 1283, 2306
BshFI	GGCC	7 cut(s)	248, 410, 1307, 1371, 2461, 2640, 2666
BshNI	GGYRCC	2 cut(s)	816, 932
BsiHKAI	GWGCWC	3 cut(s)	913, 1492, 1510
BsiHKCI	CYCGRG	1 cut(s)	1836
BsiSI	CCGG	3 cut(s)	569, 1179, 2388
BslFI	GGGAC	3 cut(s)	1454, 2370, 2603
BslI	CCNNNNNNNGG	4 cut(s)	1168, 2361, 2367, 2468
BsmAI	GTCTC	2 cut(s)	516, 2690
BsmBI	CGTCTC	1 cut(s)	2690
BsmFI	GGGAC	3 cut(s)	1454, 2370, 2603
BsnI	GGCC	7 cut(s)	248, 410, 1307, 1371, 2461, 2640, 2666
Bso31I	GGTCTC	1 cut(s)	516
BsoBI	CYCGRG	1 cut(s)	1836
Bsp1286I	GDGCHC	4 cut(s)	913, 1492, 1510, 1595
Bsp1720I	GCTNAGC	1 cut(s)	2421
Bsp19I	CCATGG	1 cut(s)	1436
BspACI	CCGC	5 cut(s)	281, 415, 677, 2304, 2306
BspANI	GGCC	7 cut(s)	248, 410, 1307, 1371, 2461, 2640, 2666
BspCNI	CTCAG	5 cut(s)	400, 439, 1690, 2434, 2746
BspFNI	CGCG	3 cut(s)	417, 1283, 2306
BspHI	TCATGA	2 cut(s)	1480, 2166
BspLI	GGNNCC	6 cut(s)	456, 818, 863, 934, 2591, 2859
BspMAI	CTGCAG	1 cut(s)	238
BspMI	ACCTGC	1 cut(s)	1789
BspPI	GGATC	6 cut(s)	856, 869, 1463, 2537, 2852, 2865
BspQI	GCTCTTC	1 cut(s)	1458
BspT107I	GGYRCC	2 cut(s)	816, 932
BspTNI	GGTCTC	1 cut(s)	516
BsrDI	GCAATG	3 cut(s)	867, 879, 2454
BsrI	ACTGG	5 cut(s)	655, 1762, 2140, 2503, 2711
BssECI	CCNNGG	4 cut(s)	33, 1436, 2073, 2304
BssHII	GCGCGC	1 cut(s)	1281
BssT1I	CCWWGG	2 cut(s)	1436, 2073
Bst2UI	CCWGG	5 cut(s)	459, 542, 585, 1869, 2362
Bst4CI	ACNGT	6 cut(s)	800, 821, 883, 1487, 2173, 2690
Bst6I	CTCTTC	5 cut(s)	63, 570, 1284, 1458, 1715
BstAPI	GCANNNNNTGC	4 cut(s)	374, 908, 1103, 2780
BstBAI	YACGTR	2 cut(s)	1175, 1964
BstC8I	GCNNGC	6 cut(s)	279, 617, 1283, 1506, 1554, 2772
BstDEI	CTNAG	8 cut(s)	387, 447, 1373, 1698, 1874, 2421, 2573, 2754
BstDSI	CCRYGG	3 cut(s)	33, 1436, 2304
BstEII	GGTNACC	2 cut(s)	571, 1544
BstF5I	GGATG	3 cut(s)	985, 1822, 2492
BstFNI	CGCG	3 cut(s)	417, 1283, 2306
BstHHI	GCGC	3 cut(s)	1283, 1285, 2732
BstMAI	GTCTC	2 cut(s)	516, 2690
BstNI	CCWGG	5 cut(s)	459, 542, 585, 1869, 2362
BstNSI	RCATGY	4 cut(s)	707, 1343, 1603, 2536
BstPAI	GACNNNNGTC	1 cut(s)	653
BstPI	GGTNACC	2 cut(s)	571, 1544
BstSCI	CCNGG	7 cut(s)	457, 540, 583, 1178, 1867, 2360, 2386
BstSFI	CTRYAG	1 cut(s)	234
BstSLI	GKGCMC	2 cut(s)	1492, 1595
BstSNI	TACGTA	1 cut(s)	1964
BstUI	CGCG	3 cut(s)	417, 1283, 2306
BstV2I	GAAGAC	3 cut(s)	503, 794, 1987
BstX2I	RGATCY	2 cut(s)	861, 2857
BstXI	CCANNNNNNTGG	2 cut(s)	219, 2109
BstYI	RGATCY	2 cut(s)	861, 2857
BsuRI	GGCC	7 cut(s)	248, 410, 1307, 1371, 2461, 2640, 2666
BtgI	CCRYGG	3 cut(s)	33, 1436, 2304
BtgZI	GCGATG	2 cut(s)	432, 899
BtsCI	GGATG	3 cut(s)	985, 1822, 2492
BtsI	GCAGTG	1 cut(s)	997
BtsIMutI	CAGTG	7 cut(s)	600, 997, 1492, 1531, 1635, 1824, 1894
BveI	ACCTGC	1 cut(s)	1789
Cac8I	GCNNGC	6 cut(s)	279, 617, 1283, 1506, 1554, 2772
CaiI	CAGNNNCTG	3 cut(s)	374, 1868, 2367
CciI	TCATGA	2 cut(s)	1480, 2166
CfoI	GCGC	3 cut(s)	1283, 1285, 2732
Cfr13I	GGNCC	4 cut(s)	1306, 1433, 2590, 2664
Cfr42I	CCGCGG	1 cut(s)	2307
CseI	GACGC	2 cut(s)	1784, 2372
DdeI	CTNAG	8 cut(s)	387, 447, 1373, 1698, 1874, 2421, 2573, 2754
DraI	TTTAAA	1 cut(s)	531
DraIII	CACNNNGTG	1 cut(s)	1270
DrdI	GACNNNNNNGTC	1 cut(s)	2597
DseDI	GACNNNNNNGTC	1 cut(s)	2597
EaeI	YGGCCR	2 cut(s)	246, 2638
Eam1104I	CTCTTC	5 cut(s)	63, 570, 1284, 1458, 1715
EarI	CTCTTC	5 cut(s)	63, 570, 1284, 1458, 1715
Eco105I	TACGTA	1 cut(s)	1964
Eco130I	CCWWGG	2 cut(s)	1436, 2073
Eco31I	GGTCTC	1 cut(s)	516
Eco32I	GATATC	2 cut(s)	490, 2442
Eco47I	GGWCC	2 cut(s)	1433, 2590
Eco57I	CTGAAG	4 cut(s)	576, 1308, 1659, 2783
Eco72I	CACGTG	1 cut(s)	1175
Eco88I	CYCGRG	1 cut(s)	1836
Eco91I	GGTNACC	2 cut(s)	571, 1544
EcoO65I	GGTNACC	2 cut(s)	571, 1544
EcoRII	CCWGG	5 cut(s)	457, 540, 583, 1867, 2360
EcoRV	GATATC	2 cut(s)	490, 2442
EcoT14I	CCWWGG	2 cut(s)	1436, 2073
ErhI	CCWWGG	2 cut(s)	1436, 2073
Esp3I	CGTCTC	1 cut(s)	2690
FaqI	GGGAC	3 cut(s)	1454, 2370, 2603
FauNDI	CATATG	1 cut(s)	1745
FbaI	TGATCA	1 cut(s)	1536
FblI	GTMKAC	1 cut(s)	2310
FokI	GGATG	3 cut(s)	992, 1829, 2499
FspBI	CTAG	3 cut(s)	1244, 1902, 2558
GlaI	GCGC	3 cut(s)	1282, 1284, 2731
GsuI	CTGGAG	2 cut(s)	2085, 2520
HaeIII	GGCC	7 cut(s)	248, 410, 1307, 1371, 2461, 2640, 2666
HapII	CCGG	3 cut(s)	569, 1179, 2388
HgaI	GACGC	2 cut(s)	1784, 2372
HhaI	GCGC	3 cut(s)	1283, 1285, 2732
Hin6I	GCGC	3 cut(s)	1281, 1283, 2730
HinP1I	GCGC	3 cut(s)	1281, 1283, 2730
HindIII	AAGCTT	1 cut(s)	1022
HinfI	GANTC	9 cut(s)	198, 208, 560, 1039, 1521, 1689, 2082, 2472, 2822
HpaII	CCGG	3 cut(s)	569, 1179, 2388
HphI	GGTGA	8 cut(s)	22, 569, 635, 1424, 1436, 1556, 1772, 2449
Hpy166II	GTNNAC	5 cut(s)	598, 658, 1490, 2311, 2681
Hpy188I	TCNGA	7 cut(s)	1044, 1159, 1627, 1849, 2477, 2802, 2885
Hpy8I	GTNNAC	5 cut(s)	598, 658, 1490, 2311, 2681
HpyCH4III	ACNGT	6 cut(s)	800, 821, 883, 1487, 2173, 2690
HpyCH4IV	ACGT	5 cut(s)	1108, 1174, 1238, 1366, 1963
HpyF3I	CTNAG	8 cut(s)	387, 447, 1373, 1698, 1874, 2421, 2573, 2754
HpySE526I	ACGT	5 cut(s)	1108, 1174, 1238, 1366, 1963
HspAI	GCGC	3 cut(s)	1281, 1283, 2730
KpnI	GGTACC	1 cut(s)	820
Ksp22I	TGATCA	1 cut(s)	1536
KspI	CCGCGG	1 cut(s)	2307
LguI	GCTCTTC	1 cut(s)	1458
LmnI	GCTCC	3 cut(s)	127, 2150, 2364
LweI	GCATC	7 cut(s)	430, 493, 1074, 1114, 1810, 1819, 2441
MaeI	CTAG	3 cut(s)	1244, 1902, 2558
MaeII	ACGT	5 cut(s)	1108, 1174, 1238, 1366, 1963
MaeIII	GTNAC	8 cut(s)	146, 571, 710, 892, 1544, 2350, 2524, 2535
MfeI	CAATTG	5 cut(s)	777, 903, 1140, 1497, 1557
MflI	RGATCY	2 cut(s)	861, 2857
MhlI	GDGCHC	4 cut(s)	913, 1492, 1510, 1595
MlsI	TGGCCA	2 cut(s)	248, 2640
MluNI	TGGCCA	2 cut(s)	248, 2640
MlyI	GAGTC	3 cut(s)	1530, 1698, 2091
MmeI	TCCRAC	3 cut(s)	2090, 2120, 2474
Mox20I	TGGCCA	2 cut(s)	248, 2640
MroXI	GAANNNNTTC	2 cut(s)	57, 564
MscI	TGGCCA	2 cut(s)	248, 2640
MseI	TTAA	8 cut(s)	530, 1056, 1185, 1419, 1530, 1859, 2046, 2742
MslI	CAYNNNNRTG	7 cut(s)	217, 872, 1485, 1598, 1707, 1746, 2286
Msp20I	TGGCCA	2 cut(s)	248, 2640
MspA1I	CMGCKG	6 cut(s)	281, 371, 446, 2306, 2425, 2828
MspI	CCGG	3 cut(s)	569, 1179, 2388
MspR9I	CCNGG	7 cut(s)	459, 542, 585, 1180, 1869, 2362, 2388
MunI	CAATTG	5 cut(s)	777, 903, 1140, 1497, 1557
MvaI	CCWGG	5 cut(s)	459, 542, 585, 1869, 2362
MvnI	CGCG	3 cut(s)	417, 1283, 2306
NciI	CCSGG	2 cut(s)	1180, 2388
NcoI	CCATGG	1 cut(s)	1436
NdeI	CATATG	1 cut(s)	1745
NlaIV	GGNNCC	6 cut(s)	456, 818, 863, 934, 2591, 2859
NmuCI	GTSAC	3 cut(s)	710, 1544, 2350
NspI	RCATGY	4 cut(s)	707, 1343, 1603, 2536
OliI	CACNNNNGTG	1 cut(s)	1746
PagI	TCATGA	2 cut(s)	1480, 2166
PalAI	GGCGCGCC	1 cut(s)	1281
PaqCI	CACCTGC	1 cut(s)	1789
PauI	GCGCGC	1 cut(s)	1281
PciI	ACATGT	2 cut(s)	1599, 2532
PciSI	GCTCTTC	1 cut(s)	1458
PdmI	GAANNNNTTC	2 cut(s)	57, 564
PfeI	GAWTC	6 cut(s)	198, 208, 560, 1039, 2472, 2822
PflFI	GACNNNGTC	1 cut(s)	2081
PflMI	CCANNNNNTGG	1 cut(s)	2367
PfoI	TCCNGGA	2 cut(s)	457, 2360
PleI	GAGTC	3 cut(s)	1529, 1697, 2090
PmaCI	CACGTG	1 cut(s)	1175
PmlI	CACGTG	1 cut(s)	1175
PpsI	GAGTC	3 cut(s)	1529, 1697, 2090
Ppu21I	YACGTR	2 cut(s)	1175, 1964
PscI	ACATGT	2 cut(s)	1599, 2532
PshAI	GACNNNNGTC	1 cut(s)	653
Psp6I	CCWGG	5 cut(s)	457, 540, 583, 1867, 2360
PspCI	CACGTG	1 cut(s)	1175
PspEI	GGTNACC	2 cut(s)	571, 1544
PspGI	CCWGG	5 cut(s)	457, 540, 583, 1867, 2360
PspN4I	GGNNCC	6 cut(s)	456, 818, 863, 934, 2591, 2859
PspPI	GGNCC	4 cut(s)	1306, 1433, 2590, 2664
PstI	CTGCAG	1 cut(s)	238
PstNI	CAGNNNCTG	3 cut(s)	374, 1868, 2367
PsuI	RGATCY	2 cut(s)	861, 2857
PsyI	GACNNNGTC	1 cut(s)	2081
PteI	GCGCGC	1 cut(s)	1281
PvuII	CAGCTG	4 cut(s)	371, 446, 2425, 2828
RseI	CAYNNNNRTG	7 cut(s)	217, 872, 1485, 1598, 1707, 1746, 2286
SacII	CCGCGG	1 cut(s)	2307
SapI	GCTCTTC	1 cut(s)	1458
SaqAI	TTAA	8 cut(s)	530, 1056, 1185, 1419, 1530, 1859, 2046, 2742
Sau96I	GGNCC	4 cut(s)	1306, 1433, 2590, 2664
ScaI	AGTACT	1 cut(s)	697
SchI	GAGTC	3 cut(s)	1530, 1698, 2091
ScrFI	CCNGG	7 cut(s)	459, 542, 585, 1180, 1869, 2362, 2388
SduI	GDGCHC	4 cut(s)	913, 1492, 1510, 1595
SfaNI	GCATC	7 cut(s)	430, 493, 1074, 1114, 1810, 1819, 2441
SfcI	CTRYAG	1 cut(s)	234
Sfr303I	CCGCGG	1 cut(s)	2307
SgrBI	CCGCGG	1 cut(s)	2307
SgsI	GGCGCGCC	1 cut(s)	1281
SinI	GGWCC	2 cut(s)	1433, 2590
SmiMI	CAYNNNNRTG	7 cut(s)	217, 872, 1485, 1598, 1707, 1746, 2286
SmlI	CTYRAG	4 cut(s)	20, 158, 185, 2852
SmoI	CTYRAG	4 cut(s)	20, 158, 185, 2852
SnaBI	TACGTA	1 cut(s)	1964
SsiI	CCGC	5 cut(s)	281, 415, 677, 2304, 2306
SspMI	CTAG	3 cut(s)	1244, 1902, 2558
StyD4I	CCNGG	7 cut(s)	457, 540, 583, 1178, 1867, 2360, 2386
StyI	CCWWGG	2 cut(s)	1436, 2073
TaaI	ACNGT	6 cut(s)	800, 821, 883, 1487, 2173, 2690
TaiI	ACGT	5 cut(s)	1111, 1177, 1241, 1369, 1966
TaqI	TCGA	9 cut(s)	329, 758, 1047, 1068, 1148, 1350, 1653, 1665, 2601
TaqII	GACCGA	2 cut(s)	698, 2607
TatI	WGTACW	3 cut(s)	597, 695, 1336
TauI	GCSGC	1 cut(s)	680
TfiI	GAWTC	6 cut(s)	198, 208, 560, 1039, 2472, 2822
Tru1I	TTAA	8 cut(s)	530, 1056, 1185, 1419, 1530, 1859, 2046, 2742
Tru9I	TTAA	8 cut(s)	530, 1056, 1185, 1419, 1530, 1859, 2046, 2742
TscAI	CASTG	7 cut(s)	600, 997, 1492, 1531, 1642, 1831, 1894
TseFI	GTSAC	3 cut(s)	710, 1544, 2350
Tsp45I	GTSAC	3 cut(s)	710, 1544, 2350
TspGWI	ACGGA	5 cut(s)	20, 50, 1263, 2552, 2750
TspRI	CASTG	7 cut(s)	600, 997, 1492, 1531, 1642, 1831, 1894
Tth111I	GACNNNGTC	1 cut(s)	2081
Van91I	CCANNNNNTGG	1 cut(s)	2367
VneI	GTGCAC	1 cut(s)	1488
VpaK11BI	GGWCC	2 cut(s)	1433, 2590
XapI	RAATTY	5 cut(s)	742, 1355, 2124, 2552, 2632
XceI	RCATGY	4 cut(s)	707, 1343, 1603, 2536
XcmI	CCANNNNNNNNNTGG	2 cut(s)	665, 964
XmiI	GTMKAC	1 cut(s)	2310
XmnI	GAANNNNTTC	2 cut(s)	57, 564
XspI	CTAG	3 cut(s)	1244, 1902, 2558
ZrmI	AGTACT	1 cut(s)	697

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.