Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0002698

Physical Location & Seq

Genomic Coordinates Forward (+)

29085474 .. 29091519

Gene Structure 6046 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00307640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2673 bp

ATGGTTGCGGAGTCGGTTGCCCTTGACGTGATCAACAAGGAAACTGTTGATCTGGAGAATGTTTCCCTGAAAGAAGTTTTTGACCATCTTAAATGCACAAAAGATGGTTTAAGCTCTGATGAAGTTAAAGAGCGGTTGGATTCATTTGGTTACAATAAGCTTGAAGAAAAGAAGGTCTTGCGTGATGGAAAATGGAGTGAAGAAGATGCTGCTATATTGGTTCCTGGAGACATAATTAGCATCAAACTTGGTGATATTGTTCCAGCTGATGCACGTCTTCTTCTTGAAGGAGATCCTCTGAAGATTGATCAGTCTGCTCTTACTGGAGAATCACTTCCAGTGACCAAAAATCCTGGTGATGGGGTTTACTCAGGATCAACATGTAAACAAGGAGAAATTGAAGCAGTTGTTATTGCAACTGGTGTCCACACTTTTTTTGGAAAGGCAGCTCACCTTGTGGAGAACACAACACACATTGGCCATTTCCAGCAGGTTTTAACATCAATTGGAAACTTCTGCATTTGTTCAATTGCTGTTGGGATGGTGATCGAAATCATAGTAATATATGGAATTCATGGAAGGGAGTACCGTGTTGGTATTGATAACCTACTTGTCCTCCTAATTGGTGGCATCCCTATTGCTATGCCAACTGTTCTTTCTGTTACAATGGCAATTGGTTCACATCGCTTGTCCCAGCAGCTGACTTTAACCTTAAACCATGTGGTATACCATTTGCGGAGTATACTAAGTCACTTCACAGAAGCATACATATATGGTAAGCCAGTCACATCAGATGAAGTTGTAATACGGTTATTTTGGTCGGGGAAGGTTTTTGCCAAAGGTGCTGACAAGGATCAGGTTATACTTATGGCTGCAAGAGCATCAAGGTTAGAGAATCAAGATGCCATTGATACAGCAATTGTTTCGATGCTTTCAGACCCTAAAGAGGCACGAGCTGGAATCAAAGAAATTCACTTTCTTCCATTCAATCCTACCGATAAAAGGACTGCACTTACGTATATTGACGGTGCTGGTAAAATGCACAGAGTCAGTAAAGGTGCACCAGAGCAGATTATTAATCTGGCCCATAACAAATCACAAATTGAAAAGAAGGTACATACAATGATCGACAAGTTTGCAGAACGTGGACTACGATCCCTTGGTGTTGCTCGCCAGGAAGTACCTGCTGGAACAAAAGACAGTCCAGGTGGCCCCTGGGAATTCGTTGGCCTTCTCCCTCTCTTTGACCCTCCTCGCCATGATAGTGCTGAAACAATCAGAAGAGCTCTGGAACTTGGTGTCAGTGTTAAAATGATCACAGGGGACCAACTTGCAATTGGTAAGGAAACAGGAAGGCGACTTGGGATGGGCACAAACATGTATCCTTCTTCAGCTCTTCTTGGTGAAAGTAAGGATGGGGATCTTGCTACTATCCCCATTGATGAACTCATTGAAAAGGCTGACGGTTTTGCTGGTGTTTTTCCTGAGCATAAATACGAGATTGTGAAGAGATTGCAAGCTCGAAAGCACATCTGTGGAATGACTGGTGATGGAGTGAATGATGCTCCAGCCTTGAAGAAAGCTGACATAGGAATTGCTGTAGCAGATTCGACAGATGCTGCCCGCAGTGCTTCTGATATTGTTTTAACAGAACCTGGGTTGAGTGTAATCATTAGTGCTGTGCTAACAAGCCGTGCAATTTTCCAGAGAATGAAAAACTATACGATATACGCCGTCTCAATCACCATACGTATTGTGCTAGGGTTTATGTTGCTGGCTGTCTTCTGGAAATTTGACTTCCCTCCTTTCATGGTTCTTGTCATTGCTGTTCTTAACGATGGTACTATCATGACCATATCTAAAGATAGGGTCAAGCCATCTCCATTTCCCGACAGTTGGAAGCTCAGTGAAATTTTTGTAACTGGGGTTGCCCTTGGTAGTTACTTGGCACTGATGACTGTTATATTCTTCTGGGCAGCCTATGAAACCAATTTCTTTCACAAACATTTTGGTGTGGAAAACTTCAATCAACATAATCTTGATATGAAAAATCCTTCTATTTCTACTCCAGTAAAAGAGAAGTTGTCTTCTGCTTTGTATCTCCAAATCAGTACCATCAGCCAGGCGTTAATATTTGTTACTCGCTCCAGGGGATGGTCCTTCACAGAAAGACCAGGCCTTTTGCTTGTAGTAGCCTTCATCATTGCTCAACTGATTGCTACAGTACTATCTGCCACTGCCAACTGGGAATTTACAAATATCAGAAGCATCGGTTGGGGTTGGACTGGTGTTATTTGGTTGTTTAACATTGCCACTTACATGCTACTTGATCCTATCAAATTTGCTGTCCGCTACGCACTCAGTGGACAGGCATGGGGTTTAGTAGTAGACCAAAGAACTGCATTTACTAGCAAAAAGGATTTTGGAAAGGAATCTCGCGAGGCTGCATGGGCAGCTGAACAGCGAACGCTACATGGACTGCACTCTGGTGAAGGGAAAATATTTTCAGAGAAGAGCACTTTTAGAGACATTAATCTCATGGCAGAAGAAGCTAAAAGGCGCGCAGAGATTGCAAGGATGAGGGAGCTTCACACTCTCAAAGGCAAGGTGGAATCATTCGCAAAGCTTAAGGGTTTGGATATTGATGCCATGAACCAAAACTACACTGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00190 map00190

Pfam Domains

Protein Families

Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

890

Amino Acids

97.98

Weight (kDa)

6.78

Isoelectric Point (pI)

33.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	20 - 58	3.7e-07	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	58 - 154	3.4e-28	P-type ATPase actuator domain
Hydrolase	PF00702	404 - 529	1.7e-10	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002698)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G60330 AT3G60330 AT3G60330 AT3G60330
fragaria_vesca	FvH4_5g19790 FvH4_7g09980
malus_domestica	MD02G1216000.v1.1 MD03G1197700.v1.1 MD03G1197800.v1.1 MD04G1058300.v1.1
prunus_persica	Prupe.2G128800_v2.0.a1 Prupe.5G062200_v2.0.a1
pyrus_communis	pycom04g05210
rosa_chinensis	RchiOBHm_Chr1g0347581 RchiOBHm_Chr4g0412601 RchiOBHm_Chr7g0204571
rosa_laevigata	RLG00000003448 RLG00000028736 RLG00000030415
rosa_multiflora	Rmu_sc0001211.1_g000115 Rmu_sc0005397.1_g000001 Rmu_sc0007327.1_g000001 Rmu_sc0021384.1_g000002 Rmu_sc0024460.1_g000001 Rmu_sc0027638.1_g000006
rosa_roxburghii	Rroxscaffold_3G00252980 Rroxscaffold_4G00307640 Rroxscaffold_4G00307710
rosa_rugosa	Rorug01G0188000 Rorug07G0085200
rosa_samantha	Rh1DG200900 Rh7AG214600 Rh7BG212100 Rh7CG227700 Rh7DG222300
rosa_wichuraiana	Rw0G013650 Rw0G018200 Rw1G017220 Rw7G018620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	481, 1192
AccB7I	CCANNNNNTGG	1 cut(s)	2154
AccBSI	CCGCTC	1 cut(s)	133
AccI	GTMKAC	3 cut(s)	726, 742, 2388
AccII	CGCG	2 cut(s)	2439, 2562
AciI	CCGC	5 cut(s)	8, 133, 736, 1624, 2350
AclWI	GGATC	6 cut(s)	287, 382, 861, 1149, 1428, 2324
AcoI	YGGCCR	1 cut(s)	478
AcsI	RAATTY	7 cut(s)	570, 969, 1220, 1790, 1911, 2249, 2339
AcuI	CTGAAG	2 cut(s)	320, 1374
AdeI	CACNNNGTG	2 cut(s)	457, 2363
AfaI	GTAC	6 cut(s)	587, 1116, 1182, 1843, 2113, 2226
AfiI	CCNNNNNNNGG	5 cut(s)	359, 946, 1002, 1896, 2154
AflII	CTTAAG	1 cut(s)	2627
AflIII	ACRYGT	2 cut(s)	380, 1377
AgsI	TTSAA	9 cut(s)	164, 287, 401, 528, 988, 1106, 1454, 1576, 2026
AjiI	CACGTC	2 cut(s)	28, 275
AjnI	CCWGG	9 cut(s)	223, 352, 1173, 1204, 1214, 1654, 2121, 2147, 2173
AjuI	GAANNNNNNNTTGG	4 cut(s)	640, 672, 2257, 2289
AleI	CACNNNNGTG	2 cut(s)	1533, 2487
Alw21I	GWGCWC	3 cut(s)	1063, 1288, 2519
Alw26I	GTCTC	3 cut(s)	222, 1741, 2520
Alw44I	GTGCAC	1 cut(s)	1059
AlwI	GGATC	6 cut(s)	287, 382, 861, 1149, 1428, 2324
AlwNI	CAGNNNCTG	3 cut(s)	700, 1619, 1655
AoxI	GGCC	5 cut(s)	478, 1083, 1210, 1228, 2176
ApaLI	GTGCAC	1 cut(s)	1059
ApeKI	GCWGC	8 cut(s)	209, 446, 697, 872, 1619, 1976, 2444, 2453
ApoI	RAATTY	7 cut(s)	570, 969, 1220, 1790, 1911, 2249, 2339
ArsI	GACNNNNNNTTYG	2 cut(s)	2331, 2363
AseI	ATTAAT	2 cut(s)	1077, 2532
Asp700I	GAANNNNTTC	1 cut(s)	333
AspLEI	GCGC	2 cut(s)	2562, 2564
AspS9I	GGNCC	4 cut(s)	1084, 1211, 1324, 2157
AsuHPI	GGTGA	8 cut(s)	263, 368, 443, 556, 1415, 1559, 1735, 2501
AvaII	GGWCC	2 cut(s)	1324, 2157
BaeGI	GKGCMC	2 cut(s)	1063, 1373
BalI	TGGCCA	1 cut(s)	480
BanII	GRGCYC	1 cut(s)	1288
BarI	GAAGNNNNNNTAC	2 cut(s)	789, 821
BauI	CACGAG	1 cut(s)	951
BbsI	GAAGAC	3 cut(s)	269, 1774, 2079
Bbv12I	GWGCWC	3 cut(s)	1063, 1288, 2519
BbvI	GCAGC	8 cut(s)	196, 458, 709, 859, 1606, 1988, 2431, 2465
BceAI	ACGGC	2 cut(s)	1677, 1718
BcgI	CGANNNNNNTGC	4 cut(s)	906, 940, 1118, 1152
BciT130I	CCWGG	9 cut(s)	225, 354, 1175, 1206, 1216, 1656, 2123, 2149, 2175
BciVI	GTATCC	1 cut(s)	1392
BclI	TGATCA	3 cut(s)	30, 307, 1314
BcoDI	GTCTC	3 cut(s)	222, 1741, 2520
BfaI	CTAG	2 cut(s)	1760, 2409
BfmI	CTRYAG	2 cut(s)	1599, 2220
BfrI	CTTAAG	1 cut(s)	2627
BfuAI	ACCTGC	2 cut(s)	481, 1192
BfuI	GTATCC	1 cut(s)	1392
BisI	GCNGC	8 cut(s)	210, 447, 698, 873, 1620, 1977, 2445, 2454
BlsI	GCNGC	8 cut(s)	211, 448, 699, 874, 1621, 1978, 2446, 2455
BmcAI	AGTACT	1 cut(s)	2226
Bme1390I	CCNGG	9 cut(s)	225, 354, 1175, 1206, 1216, 1656, 2123, 2149, 2175
Bme18I	GGWCC	2 cut(s)	1324, 2157
BmgBI	CACGTC	2 cut(s)	28, 275
BmgT120I	GGNCC	4 cut(s)	1084, 1211, 1324, 2157
BmiI	GGNNCC	3 cut(s)	222, 1213, 1325
BmrFI	CCNGG	9 cut(s)	225, 354, 1175, 1206, 1216, 1656, 2123, 2149, 2175
BmrI	ACTGGG	2 cut(s)	1932, 2254
BmuI	ACTGGG	2 cut(s)	1932, 2254
BpiI	GAAGAC	3 cut(s)	269, 1774, 2079
BpmI	CTGGAG	6 cut(s)	74, 246, 345, 1551, 2052, 2131
Bpu10I	CCTNAGC	1 cut(s)	1485
BsaAI	YACGTR	2 cut(s)	1017, 1751
BsaBI	GATNNNNATC	3 cut(s)	545, 551, 1419
BsaJI	CCNNGG	6 cut(s)	1159, 1214, 1215, 1655, 1933, 2148
BsaXI	ACNNNNNCTCC	2 cut(s)	600, 630
Bsc4I	CCNNNNNNNGG	5 cut(s)	359, 946, 1002, 1896, 2154
Bse1I	ACTGG	9 cut(s)	328, 338, 424, 782, 1549, 1927, 2069, 2249, 2290
Bse3DI	GCAATG	3 cut(s)	1821, 2202, 2307
Bse8I	GATNNNNATC	3 cut(s)	545, 551, 1419
BseBI	CCWGG	9 cut(s)	225, 354, 1175, 1206, 1216, 1656, 2123, 2149, 2175
BseDI	CCNNGG	6 cut(s)	1159, 1214, 1215, 1655, 1933, 2148
BseGI	GGATG	6 cut(s)	546, 630, 1371, 1420, 2159, 2583
BseJI	GATNNNNATC	3 cut(s)	545, 551, 1419
BseLI	CCNNNNNNNGG	5 cut(s)	359, 946, 1002, 1896, 2154
BseMI	GCAATG	3 cut(s)	1821, 2202, 2307
BseMII	CTCAG	4 cut(s)	384, 1476, 1918, 2374
BseNI	ACTGG	9 cut(s)	328, 338, 424, 782, 1549, 1927, 2069, 2249, 2290
BsePI	GCGCGC	1 cut(s)	2560
BseRI	GAGGAG	1 cut(s)	1242
BseSI	GKGCMC	2 cut(s)	1063, 1373
BseXI	GCAGC	8 cut(s)	196, 458, 709, 859, 1606, 1988, 2431, 2465
BseYI	CCCAGC	1 cut(s)	693
BsgI	GTGCAG	2 cut(s)	993, 2465
Bsh1236I	CGCG	2 cut(s)	2439, 2562
BshFI	GGCC	5 cut(s)	480, 1085, 1212, 1230, 2178
BsiHKAI	GWGCWC	3 cut(s)	1063, 1288, 2519
BslFI	GGGAC	2 cut(s)	676, 1337
BslI	CCNNNNNNNGG	5 cut(s)	359, 946, 1002, 1896, 2154
BsmAI	GTCTC	3 cut(s)	222, 1741, 2520
BsmBI	CGTCTC	1 cut(s)	1741
BsmFI	GGGAC	2 cut(s)	676, 1337
BsnI	GGCC	5 cut(s)	480, 1085, 1212, 1230, 2178
Bsp1286I	GDGCHC	4 cut(s)	1063, 1288, 1373, 2519
Bsp68I	TCGCGA	1 cut(s)	2439
BspACI	CCGC	5 cut(s)	8, 133, 736, 1624, 2350
BspANI	GGCC	5 cut(s)	480, 1085, 1212, 1230, 2178
BspCNI	CTCAG	4 cut(s)	383, 1477, 1917, 2373
BspFNI	CGCG	2 cut(s)	2439, 2562
BspHI	TCATGA	1 cut(s)	1848
BspLI	GGNNCC	3 cut(s)	222, 1213, 1325
BspMI	ACCTGC	2 cut(s)	481, 1192
BspPI	GGATC	6 cut(s)	287, 382, 861, 1149, 1428, 2324
BspQI	GCTCTTC	3 cut(s)	1276, 1401, 2507
BspTI	CTTAAG	1 cut(s)	2627
BsrBI	CCGCTC	1 cut(s)	133
BsrDI	GCAATG	3 cut(s)	1821, 2202, 2307
BsrI	ACTGG	9 cut(s)	328, 338, 424, 782, 1549, 1927, 2069, 2249, 2290
BssECI	CCNNGG	6 cut(s)	1159, 1214, 1215, 1655, 1933, 2148
BssHII	GCGCGC	1 cut(s)	2560
BssNAI	GTATAC	2 cut(s)	727, 743
BssSI	CACGAG	1 cut(s)	951
BssT1I	CCWWGG	2 cut(s)	1159, 1933
Bst1107I	GTATAC	2 cut(s)	727, 743
Bst2BI	CACGAG	1 cut(s)	951
Bst2UI	CCWGG	9 cut(s)	225, 354, 1175, 1206, 1216, 1656, 2123, 2149, 2175
Bst6I	CTCTTC	4 cut(s)	1276, 1401, 1502, 2507
BstAFI	CTTAAG	1 cut(s)	2627
BstAPI	GCANNNNNTGC	1 cut(s)	2570
BstBAI	YACGTR	2 cut(s)	1017, 1751
BstC8I	GCNNGC	5 cut(s)	1171, 1518, 1624, 1776, 2562
BstDEI	CTNAG	5 cut(s)	370, 746, 1485, 1904, 2360
BstF5I	GGATG	6 cut(s)	546, 630, 1371, 1420, 2159, 2583
BstFNI	CGCG	2 cut(s)	2439, 2562
BstHHI	GCGC	2 cut(s)	2562, 2564
BstMAI	GTCTC	3 cut(s)	222, 1741, 2520
BstMWI	GCNNNNNNNGC	7 cut(s)	842, 878, 1628, 2450, 2453, 2549, 2570
BstNI	CCWGG	9 cut(s)	225, 354, 1175, 1206, 1216, 1656, 2123, 2149, 2175
BstNSI	RCATGY	3 cut(s)	384, 1381, 2323
BstSCI	CCNGG	9 cut(s)	223, 352, 1173, 1204, 1214, 1654, 2121, 2147, 2173
BstSFI	CTRYAG	2 cut(s)	1599, 2220
BstSLI	GKGCMC	2 cut(s)	1063, 1373
BstSNI	TACGTA	2 cut(s)	1017, 1751
BstUI	CGCG	2 cut(s)	2439, 2562
BstV1I	GCAGC	8 cut(s)	196, 458, 709, 859, 1606, 1988, 2431, 2465
BstV2I	GAAGAC	3 cut(s)	269, 1774, 2079
BstX2I	RGATCY	2 cut(s)	292, 1420
BstYI	RGATCY	2 cut(s)	292, 1420
BstZ17I	GTATAC	2 cut(s)	727, 743
BsuI	GTATCC	1 cut(s)	1392
BsuRI	GGCC	5 cut(s)	480, 1085, 1212, 1230, 2178
BtgZI	GCGATG	1 cut(s)	668
BtrI	CACGTC	2 cut(s)	28, 275
BtsCI	GGATG	6 cut(s)	546, 630, 1371, 1420, 2159, 2583
BtsI	GCAGTG	2 cut(s)	1633, 2235
BtsIMutI	CAGTG	8 cut(s)	345, 1309, 1633, 1912, 1949, 2235, 2368, 2664
BtuMI	TCGCGA	1 cut(s)	2439
BveI	ACCTGC	2 cut(s)	481, 1192
Cac8I	GCNNGC	5 cut(s)	1171, 1518, 1624, 1776, 2562
CaiI	CAGNNNCTG	3 cut(s)	700, 1619, 1655
CciI	TCATGA	1 cut(s)	1848
CfoI	GCGC	2 cut(s)	2562, 2564
Cfr13I	GGNCC	4 cut(s)	1084, 1211, 1324, 2157
Csp6I	GTAC	6 cut(s)	586, 1115, 1181, 1842, 2112, 2225
CviQI	GTAC	6 cut(s)	586, 1115, 1181, 1842, 2112, 2225
DdeI	CTNAG	5 cut(s)	370, 746, 1485, 1904, 2360
DraIII	CACNNNGTG	2 cut(s)	457, 2363
EaeI	YGGCCR	1 cut(s)	478
Eam1104I	CTCTTC	4 cut(s)	1276, 1401, 1502, 2507
EarI	CTCTTC	4 cut(s)	1276, 1401, 1502, 2507
Ecl136II	GAGCTC	1 cut(s)	1286
Eco105I	TACGTA	2 cut(s)	1017, 1751
Eco130I	CCWWGG	2 cut(s)	1159, 1933
Eco147I	AGGCCT	1 cut(s)	2178
Eco24I	GRGCYC	1 cut(s)	1288
Eco47I	GGWCC	2 cut(s)	1324, 2157
Eco53kI	GAGCTC	1 cut(s)	1286
Eco57I	CTGAAG	2 cut(s)	320, 1374
EcoICRI	GAGCTC	1 cut(s)	1286
EcoRI	GAATTC	2 cut(s)	570, 1220
EcoRII	CCWGG	9 cut(s)	223, 352, 1173, 1204, 1214, 1654, 2121, 2147, 2173
EcoT14I	CCWWGG	2 cut(s)	1159, 1933
EcoT38I	GRGCYC	1 cut(s)	1288
ErhI	CCWWGG	2 cut(s)	1159, 1933
Esp3I	CGTCTC	1 cut(s)	1741
FalI	AAGNNNNNCTT	2 cut(s)	161, 193
FaqI	GGGAC	2 cut(s)	676, 1337
FauI	CCCGC	1 cut(s)	1631
FbaI	TGATCA	3 cut(s)	30, 307, 1314
FblI	GTMKAC	3 cut(s)	726, 742, 2388
Fnu4HI	GCNGC	8 cut(s)	210, 447, 698, 873, 1620, 1977, 2445, 2454
FokI	GGATG	6 cut(s)	553, 617, 1378, 1427, 2166, 2590
FriOI	GRGCYC	1 cut(s)	1288
Fsp4HI	GCNGC	8 cut(s)	210, 447, 698, 873, 1620, 1977, 2445, 2454
FspBI	CTAG	2 cut(s)	1760, 2409
GlaI	GCGC	2 cut(s)	2561, 2563
GluI	GCNGC	8 cut(s)	210, 447, 698, 873, 1620, 1977, 2445, 2454
GsaI	CCCAGC	1 cut(s)	697
GsuI	CTGGAG	6 cut(s)	74, 246, 345, 1551, 2052, 2131
HaeIII	GGCC	5 cut(s)	480, 1085, 1212, 1230, 2178
HhaI	GCGC	2 cut(s)	2562, 2564
Hin6I	GCGC	2 cut(s)	2560, 2562
HinP1I	GCGC	2 cut(s)	2560, 2562
HindIII	AAGCTT	2 cut(s)	158, 2624
HinfI	GANTC	9 cut(s)	11, 140, 329, 895, 960, 1047, 1607, 2432, 2612
HphI	GGTGA	8 cut(s)	263, 368, 443, 556, 1415, 1559, 1735, 2501
Hpy188I	TCNGA	8 cut(s)	118, 300, 793, 937, 1280, 1636, 2264, 2509
HpyCH4IV	ACGT	5 cut(s)	27, 274, 1016, 1144, 1750
HpyF10VI	GCNNNNNNNGC	7 cut(s)	842, 878, 1628, 2450, 2453, 2549, 2570
HpyF3I	CTNAG	5 cut(s)	370, 746, 1485, 1904, 2360
HpySE526I	ACGT	5 cut(s)	27, 274, 1016, 1144, 1750
HspAI	GCGC	2 cut(s)	2560, 2562
Ksp22I	TGATCA	3 cut(s)	30, 307, 1314
LguI	GCTCTTC	3 cut(s)	1276, 1401, 2507
LmnI	GCTCC	3 cut(s)	1570, 2150, 2584
Lsp1109I	GCAGC	8 cut(s)	196, 458, 709, 859, 1606, 1988, 2431, 2465
MaeI	CTAG	2 cut(s)	1760, 2409
MaeII	ACGT	5 cut(s)	27, 274, 1016, 1144, 1750
MaeIII	GTNAC	8 cut(s)	149, 340, 661, 749, 784, 1918, 1940, 2137
MbiI	CCGCTC	1 cut(s)	133
MfeI	CAATTG	5 cut(s)	504, 528, 672, 918, 1335
MflI	RGATCY	2 cut(s)	292, 1420
MhlI	GDGCHC	4 cut(s)	1063, 1288, 1373, 2519
MlsI	TGGCCA	1 cut(s)	480
MluNI	TGGCCA	1 cut(s)	480
MlyI	GAGTC	2 cut(s)	20, 1056
MmeI	TCCRAC	3 cut(s)	117, 1877, 2261
MnlI	CCTC	9 cut(s)	306, 626, 940, 1248, 1260, 1263, 1812, 2434, 2574
Mox20I	TGGCCA	1 cut(s)	480
MroXI	GAANNNNTTC	1 cut(s)	333
MscI	TGGCCA	1 cut(s)	480
MslI	CAYNNNNRTG	6 cut(s)	1263, 1376, 1533, 2010, 2318, 2487
Msp20I	TGGCCA	1 cut(s)	480
MspA1I	CMGCKG	3 cut(s)	266, 700, 2456
MspCI	CTTAAG	1 cut(s)	2627
MspR9I	CCNGG	9 cut(s)	225, 354, 1175, 1206, 1216, 1656, 2123, 2149, 2175
MunI	CAATTG	5 cut(s)	504, 528, 672, 918, 1335
MvaI	CCWGG	9 cut(s)	225, 354, 1175, 1206, 1216, 1656, 2123, 2149, 2175
MvnI	CGCG	2 cut(s)	2439, 2562
MwoI	GCNNNNNNNGC	7 cut(s)	842, 878, 1628, 2450, 2453, 2549, 2570
NlaIV	GGNNCC	3 cut(s)	222, 1213, 1325
NmuCI	GTSAC	3 cut(s)	340, 749, 784
NruI	TCGCGA	1 cut(s)	2439
NspI	RCATGY	3 cut(s)	384, 1381, 2323
OliI	CACNNNNGTG	2 cut(s)	1533, 2487
PagI	TCATGA	1 cut(s)	1848
PasI	CCCWGGG	1 cut(s)	1215
PauI	GCGCGC	1 cut(s)	2560
PceI	AGGCCT	1 cut(s)	2178
PciI	ACATGT	2 cut(s)	380, 1377
PciSI	GCTCTTC	3 cut(s)	1276, 1401, 2507
PcsI	WCGNNNNNNNCGW	1 cut(s)	1150
PdmI	GAANNNNTTC	1 cut(s)	333
PfeI	GAWTC	7 cut(s)	140, 329, 895, 960, 1607, 2432, 2612
PflMI	CCANNNNNTGG	1 cut(s)	2154
PfoI	TCCNGGA	1 cut(s)	223
PkrI	GCNGC	8 cut(s)	211, 448, 699, 874, 1621, 1978, 2446, 2455
PleI	GAGTC	2 cut(s)	19, 1055
PpsI	GAGTC	2 cut(s)	19, 1055
Ppu21I	YACGTR	2 cut(s)	1017, 1751
PscI	ACATGT	2 cut(s)	380, 1377
PshBI	ATTAAT	2 cut(s)	1077, 2532
Psp124BI	GAGCTC	1 cut(s)	1288
Psp6I	CCWGG	9 cut(s)	223, 352, 1173, 1204, 1214, 1654, 2121, 2147, 2173
PspFI	CCCAGC	1 cut(s)	693
PspGI	CCWGG	9 cut(s)	223, 352, 1173, 1204, 1214, 1654, 2121, 2147, 2173
PspN4I	GGNNCC	3 cut(s)	222, 1213, 1325
PspPI	GGNCC	4 cut(s)	1084, 1211, 1324, 2157
PsrI	GAACNNNNNNTAC	5 cut(s)	1134, 1166, 2389, 2421, 2645
PstNI	CAGNNNCTG	3 cut(s)	700, 1619, 1655
PsuI	RGATCY	2 cut(s)	292, 1420
PteI	GCGCGC	1 cut(s)	2560
PvuII	CAGCTG	3 cut(s)	266, 700, 2456
RruI	TCGCGA	1 cut(s)	2439
RsaI	GTAC	6 cut(s)	587, 1116, 1182, 1843, 2113, 2226
RsaNI	GTAC	6 cut(s)	586, 1115, 1181, 1842, 2112, 2225
RseI	CAYNNNNRTG	6 cut(s)	1263, 1376, 1533, 2010, 2318, 2487
SacI	GAGCTC	1 cut(s)	1288
SapI	GCTCTTC	3 cut(s)	1276, 1401, 2507
SatI	GCNGC	8 cut(s)	210, 447, 698, 873, 1620, 1977, 2445, 2454
Sau96I	GGNCC	4 cut(s)	1084, 1211, 1324, 2157
ScaI	AGTACT	1 cut(s)	2226
SchI	GAGTC	2 cut(s)	20, 1056
ScrFI	CCNGG	9 cut(s)	225, 354, 1175, 1206, 1216, 1656, 2123, 2149, 2175
SduI	GDGCHC	4 cut(s)	1063, 1288, 1373, 2519
SfcI	CTRYAG	2 cut(s)	1599, 2220
SinI	GGWCC	2 cut(s)	1324, 2157
SmiMI	CAYNNNNRTG	6 cut(s)	1263, 1376, 1533, 2010, 2318, 2487
SmlI	CTYRAG	1 cut(s)	2627
SmoI	CTYRAG	1 cut(s)	2627
SnaBI	TACGTA	2 cut(s)	1017, 1751
SseBI	AGGCCT	1 cut(s)	2178
SsiI	CCGC	5 cut(s)	8, 133, 736, 1624, 2350
SspI	AATATT	2 cut(s)	2133, 2502
SspMI	CTAG	2 cut(s)	1760, 2409
SstI	GAGCTC	1 cut(s)	1288
StuI	AGGCCT	1 cut(s)	2178
StyD4I	CCNGG	9 cut(s)	223, 352, 1173, 1204, 1214, 1654, 2121, 2147, 2173
StyI	CCWWGG	2 cut(s)	1159, 1933
TaiI	ACGT	5 cut(s)	30, 277, 1019, 1147, 1753
TaqI	TCGA	5 cut(s)	549, 926, 1128, 1522, 1610
TatI	WGTACW	1 cut(s)	2224
TfiI	GAWTC	7 cut(s)	140, 329, 895, 960, 1607, 2432, 2612
TscAI	CASTG	8 cut(s)	345, 1309, 1633, 1912, 1956, 2242, 2368, 2671
TseFI	GTSAC	3 cut(s)	340, 749, 784
TseI	GCWGC	8 cut(s)	209, 446, 697, 872, 1619, 1976, 2444, 2453
Tsp45I	GTSAC	3 cut(s)	340, 749, 784
TspRI	CASTG	8 cut(s)	345, 1309, 1633, 1912, 1956, 2242, 2368, 2671
Van91I	CCANNNNNTGG	1 cut(s)	2154
Vha464I	CTTAAG	1 cut(s)	2627
VneI	GTGCAC	1 cut(s)	1059
VpaK11BI	GGWCC	2 cut(s)	1324, 2157
VspI	ATTAAT	2 cut(s)	1077, 2532
XapI	RAATTY	7 cut(s)	570, 969, 1220, 1790, 1911, 2249, 2339
XceI	RCATGY	3 cut(s)	384, 1381, 2323
XcmI	CCANNNNNNNNNTGG	3 cut(s)	434, 1212, 1334
XmiI	GTMKAC	3 cut(s)	726, 742, 2388
XmnI	GAANNNNTTC	1 cut(s)	333
XspI	CTAG	2 cut(s)	1760, 2409
ZrmI	AGTACT	1 cut(s)	2226

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.